MD14G1006700.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001328
Physical Location & Seq
Forward (+)
737154 .. 740466
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1006700.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGCAAAACGCTCTCTCTGTTCTCTGCCTTTGGTTCTTGCTATCATTATATTTTCAGTTCATGATTCCGAACAGCACCAATCCTCCGAAGCTCAGACCCTTTTGAGAATTACGCGACTCCTAAACCTTCCAACCGTCTTGAAAAGCTTGAATAACTACACAGATTTTTGCAACTTTGAAGTGAATTCTTCTTTTGCGGTCTCATGCTACGAAGGGAACATAACCCAGCTGCATATAATCGGCAAAAAACCTGCTCCTTTGTTGCCCCGAAACTTTTCAATTGATTCCTTTGTAACAACTCTGGTTAAGCTTCCGAGCTTGAAGGTCCTCACATTGGTCTCTCTTGGTTTATGGGGAAACTTGCCTGGTAAAATCGCGCGTTTATCATCACTCGAAATACTTAATGTGACTTCAAATTTCTTGTATGGCACCATCCCTCCAGAGCTTTCCTCTCTAACAAGCCTCCAGACGCTTATACTCGATGACAACATGTTTTCTGGACTGCTTCCAGAAGGGCTGGGTTCACTTCCGGTTTTATCTGTTTTGAGTTTGAAGAAGAACTTGTTCAATTCATCCTTGCCGAGTTCATTGAGTGAGTTGGAAAGTCTTAGACTTCTCGGGCTTTCTCATAACCACTTCTATGGGGAACTGCCTGATTTTAGTCGTTTGACGAACCTTCAAGTTCTTGAAGTGGGAGATAATGCTTTTGGACCTCAGTTTCCGAAACTTGGGAAAAAGTTGGTCACCCTTGTACTGAGAAAGAACAAATTTCGGTCTTCCATCCCTCCTGAATTGAGTTCCTATTATCAGCTACAATGTCTAGACGTTTCTTCCAACATGTTCGTGGGGCCATTTCCTCCATCGCTGCTATCTCTTCCTTCGATTACTTACCTTAATTTTTCAGGAAATAAATTCACAGGAATGCTTTTCGAAAATTCATCTTGCAATGCTGAACTTAAAGTTGTGGATTTATCCTTGAATCTTTTGACTGGAAGCTTGCCAAAATGTCTTGAGTCGGATTCTAAGAACAAGGTTTTCCTGTATGCAAGCAATTGTTTAGCTACTAGTAATCAAAATCAACATCCGCTTCCATTTTGCCACAATGAGGCGTTAGCTGTTGGAATAGTACCTGACCGAAGCAAGCGAAAGCAAGCCTCCAAAGCAGCCCTTGCCTTGGGTATAATTGGAGCACTTTTCGGAGGTGTTCTACTTGTCGGTCTAATCTTCTTCGTAAATAGAAGAATGAATACCAACAAGACAATGAAGAAATCTCCAACAAGATCAATAACAGAGAATGCATCAAGTGGATATACCTCAAAGTTACTATCAGATGCAAGATATATATCTCAAACAATGAAGACGGGAGCGCTTGGCCTTCCAGGTTATCGAACCTTTTCACTCGAGGAACTTGAGGACGCTACACAAAACTTTGATACATCTACTTTCATGGGTGAAGGCTCACATGGACAGATGTACAGAGGCCAGTTGAAGGATGGTTCTTTTGTTGCCATCAGATGTTTGAAAATGAAGGGAAGTCACAGCACTGAAATTTTCATGCACCATATAGAGCTAATTATGAAACTACGGCATCGCCATTTGGTCAGCGCACTTGGACACTGCTTTGAGTGTTACTTGGATGATTCGAGCGTCAGCAGAATATTTCTTGTCTTCGAATATGTACCAAATGGCACACTTCGGAGCTGGATATCCGGGGGACATCCCAGACGATCACTTGCGTGGACACAACGCATAGCAGCTGCAATAGGCGTAGCAAAGGGCATTCAGTTTTTGCACACGGGTATCATGCCTGGTGTATTTTCAAATAATCTTAAAATAACAGACATTTTACTGGATCAGAATTTTGTTGCGAAAATTAGCAGTTACAACCTTCCTCTGTTAGAAGAAAATATGGAACAGGTAAAACATTATGTTGTAATTAATTCATTGGGGGATTGTCCATTCGATTTCTCGAGGCAAATCATTATGCACTGGTTTTCAGATATGCTCCTCCTCGCCGGGTGGTCATGGAGGCTCTTCAAGCGGATCTGTAACTTCCAGTACCGGTGCAAGGTCTGCAATGAAAATATTCAATCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

700

Amino Acids

77.79

Weight (kDa)

9.08

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 154 - 213 1e-06 Leucine rich repeat
Pkinase PF00069 480 - 630 1.1e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 481 - 634 3.6e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 428
AccII CGCG 3 cut(s) 115, 377, 379
AciI CCGC 3 cut(s) 197, 1085, 2041
AclWI GGATC 2 cut(s) 1860, 2051
AcsI RAATTY 7 cut(s) 184, 415, 767, 911, 934, 1548, 1858
AfaI GTAC 5 cut(s) 753, 1128, 1475, 1680, 2060
AfeI AGCGCT 1 cut(s) 1368
AfiI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 2068
AflIII ACRYGT 2 cut(s) 489, 837
AgeI ACCGGT 1 cut(s) 2061
AhlI ACTAGT 1 cut(s) 1064
AjnI CCWGG 3 cut(s) 364, 1378, 1807
AleI CACNNNNGTG 1 cut(s) 1735
Alw21I GWGCWC 1 cut(s) 1192
Alw26I GTCTC 2 cut(s) 205, 343
AlwI GGATC 2 cut(s) 1860, 2051
Ama87I CYCGRG 3 cut(s) 617, 1400, 1969
Aor51HI AGCGCT 1 cut(s) 1368
AoxI GGCC 3 cut(s) 848, 1372, 1480
ApeKI GCWGC 5 cut(s) 229, 865, 1163, 1754, 1757
ApoI RAATTY 7 cut(s) 184, 415, 767, 911, 934, 1548, 1858
AseI ATTAAT 1 cut(s) 1938
AsiGI ACCGGT 1 cut(s) 2061
Asp700I GAANNNNTTC 4 cut(s) 274, 563, 1393, 2085
AspLEI GCGC 3 cut(s) 379, 1369, 1607
AspS9I GGNCC 3 cut(s) 325, 710, 848
AsuC2I CCSGG 2 cut(s) 1711, 2017
AsuHPI GGTGA 2 cut(s) 736, 1463
AsuII TTCGAA 2 cut(s) 930, 1671
AvaI CYCGRG 3 cut(s) 617, 1400, 1969
AvaII GGWCC 2 cut(s) 325, 710
BaeI ACNNNNGTAYC 2 cut(s) 1783, 1816
BanI GGYRCC 1 cut(s) 428
BarI GAAGNNNNNNTAC 2 cut(s) 1231, 1263
BbsI GAAGAC 3 cut(s) 768, 1364, 1660
Bbv12I GWGCWC 1 cut(s) 1192
BbvI GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BccI CCATC 5 cut(s) 440, 788, 868, 1487, 1517
BceAI ACGGC 1 cut(s) 1601
BciT130I CCWGG 3 cut(s) 366, 1380, 1809
BcnI CCSGG 2 cut(s) 1711, 2017
BcoDI GTCTC 2 cut(s) 205, 343
BcuI ACTAGT 1 cut(s) 1064
BfaI CTAG 2 cut(s) 821, 1065
BfoI RGCGCY 1 cut(s) 1370
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
BlsI GCNGC 5 cut(s) 231, 867, 1165, 1756, 1759
Bme1390I CCNGG 5 cut(s) 366, 1380, 1711, 1809, 2017
Bme18I GGWCC 2 cut(s) 325, 710
BmeT110I CYCGRG 3 cut(s) 617, 1400, 1969
BmgT120I GGNCC 3 cut(s) 325, 710, 848
BmiI GGNNCC 2 cut(s) 430, 849
BmrFI CCNGG 5 cut(s) 366, 1380, 1711, 1809, 2017
BmsI GCATC 3 cut(s) 1307, 1321, 1597
BpiI GAAGAC 3 cut(s) 768, 1364, 1660
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 2 cut(s) 423, 449
Bpu14I TTCGAA 2 cut(s) 930, 1671
BpuEI CTTGAG 2 cut(s) 1031, 1430
BpuMI CCSGG 2 cut(s) 1711, 2017
BsaBI GATNNNNATC 1 cut(s) 1342
BsaI GGTCTC 2 cut(s) 205, 343
BsaJI CCNNGG 2 cut(s) 1173, 1710
BsaWI WCCGGW 2 cut(s) 529, 2061
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 421, 451
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 2068
Bse118I RCCGGY 1 cut(s) 2061
Bse1I ACTGG 5 cut(s) 994, 1483, 1854, 1994, 2056
Bse3DI GCAATG 2 cut(s) 952, 2083
Bse8I GATNNNNATC 1 cut(s) 1342
BseBI CCWGG 3 cut(s) 366, 1380, 1809
BseDI CCNNGG 2 cut(s) 1173, 1710
BseGI GGATG 7 cut(s) 432, 572, 780, 1081, 1498, 1642, 1717
BseJI GATNNNNATC 1 cut(s) 1342
BseLI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 2068
BseMI GCAATG 2 cut(s) 952, 2083
BseMII CTCAG 3 cut(s) 107, 728, 746
BseNI ACTGG 5 cut(s) 994, 1483, 1854, 1994, 2056
BseRI GAGGAG 2 cut(s) 1997, 2000
BseXI GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BseYI CCCAGC 2 cut(s) 225, 517
Bsh1236I CGCG 3 cut(s) 115, 377, 379
BshFI GGCC 3 cut(s) 850, 1374, 1482
BshNI GGYRCC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 2061
BsiHKAI GWGCWC 1 cut(s) 1192
BsiHKCI CYCGRG 3 cut(s) 617, 1400, 1969
BsiSI CCGG 4 cut(s) 530, 1710, 2016, 2062
BslFI GGGAC 1 cut(s) 1728
BslI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 2068
BsmAI GTCTC 2 cut(s) 205, 343
BsmFI GGGAC 1 cut(s) 1728
BsmI GAATGC 3 cut(s) 927, 1300, 1779
BsnI GGCC 3 cut(s) 850, 1374, 1482
Bso31I GGTCTC 2 cut(s) 205, 343
BsoBI CYCGRG 3 cut(s) 617, 1400, 1969
Bsp119I TTCGAA 2 cut(s) 930, 1671
Bsp1286I GDGCHC 1 cut(s) 1192
Bsp1407I TGTACA 1 cut(s) 1473
Bsp143I GATC 4 cut(s) 1280, 1727, 1852, 2043
BspACI CCGC 3 cut(s) 197, 1085, 2041
BspANI GGCC 3 cut(s) 850, 1374, 1482
BspCNI CTCAG 3 cut(s) 106, 727, 747
BspFNI CGCG 3 cut(s) 115, 377, 379
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 430, 849
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 2 cut(s) 1860, 2051
BspQI GCTCTTC 1 cut(s) 2039
BspT104I TTCGAA 2 cut(s) 930, 1671
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 2 cut(s) 205, 343
BsrDI GCAATG 2 cut(s) 952, 2083
BsrFI RCCGGY 1 cut(s) 2061
BsrGI TGTACA 1 cut(s) 1473
BsrI ACTGG 5 cut(s) 994, 1483, 1854, 1994, 2056
BssAI RCCGGY 1 cut(s) 2061
BssECI CCNNGG 2 cut(s) 1173, 1710
BssMI GATC 4 cut(s) 1280, 1727, 1852, 2043
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 3 cut(s) 366, 1380, 1809
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 879, 2039
BstAPI GCANNNNNTGC 2 cut(s) 1169, 2073
BstAUI TGTACA 1 cut(s) 1473
BstBI TTCGAA 2 cut(s) 930, 1671
BstC8I GCNNGC 4 cut(s) 998, 1048, 1142, 1152
BstDEI CTNAG 5 cut(s) 93, 608, 714, 755, 1023
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 7 cut(s) 432, 572, 780, 1081, 1498, 1642, 1717
BstFNI CGCG 3 cut(s) 115, 377, 379
BstH2I RGCGCY 1 cut(s) 1370
BstHHI GCGC 3 cut(s) 379, 1369, 1607
BstKTI GATC 4 cut(s) 1283, 1730, 1855, 2046
BstMAI GTCTC 2 cut(s) 205, 343
BstMBI GATC 4 cut(s) 1280, 1727, 1852, 2043
BstMWI GCNNNNNNNGC 4 cut(s) 1160, 1169, 2038, 2073
BstNI CCWGG 3 cut(s) 366, 1380, 1809
BstNSI RCATGY 2 cut(s) 493, 841
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 5 cut(s) 364, 1378, 1709, 1807, 2015
BstUI CGCG 3 cut(s) 115, 377, 379
BstV1I GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BstV2I GAAGAC 3 cut(s) 768, 1364, 1660
BstX2I RGATCY 1 cut(s) 2043
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 1 cut(s) 2043
BsuRI GGCC 3 cut(s) 850, 1374, 1482
BtgZI GCGATG 2 cut(s) 846, 1574
BtsCI GGATG 7 cut(s) 432, 572, 780, 1081, 1498, 1642, 1717
BtsI GCAGTG 1 cut(s) 1615
BtsIMutI CAGTG 3 cut(s) 1542, 1615, 1987
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 998, 1048, 1142, 1152
CciI TCATGA 1 cut(s) 61
CfoI GCGC 3 cut(s) 379, 1369, 1607
Cfr10I RCCGGY 1 cut(s) 2061
Cfr13I GGNCC 3 cut(s) 325, 710, 848
CseI GACGC 3 cut(s) 478, 1424, 1636
Csp6I GTAC 5 cut(s) 752, 1127, 1474, 1679, 2059
CspAI ACCGGT 1 cut(s) 2061
CviAII CATG 9 cut(s) 62, 204, 490, 838, 1447, 1463, 1555, 1804, 2025
CviQI GTAC 5 cut(s) 752, 1127, 1474, 1679, 2059
DdeI CTNAG 5 cut(s) 93, 608, 714, 755, 1023
DpnI GATC 4 cut(s) 1282, 1729, 1854, 2045
DpnII GATC 4 cut(s) 1280, 1727, 1852, 2043
Eam1104I CTCTTC 2 cut(s) 879, 2039
EarI CTCTTC 2 cut(s) 879, 2039
Eco130I CCWWGG 1 cut(s) 1173
Eco31I GGTCTC 2 cut(s) 205, 343
Eco32I GATATC 1 cut(s) 1707
Eco47I GGWCC 2 cut(s) 325, 710
Eco47III AGCGCT 1 cut(s) 1368
Eco88I CYCGRG 3 cut(s) 617, 1400, 1969
Eco91I GGTNACC 1 cut(s) 742
EcoO109I RGGNCCY 1 cut(s) 325
EcoO65I GGTNACC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 364, 1378, 1807
EcoRV GATATC 1 cut(s) 1707
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 1 cut(s) 1300
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 9 cut(s) 65, 207, 493, 841, 1450, 1466, 1558, 1807, 2028
FalI AAGNNNNNCTT 4 cut(s) 545, 577, 1152, 1184
FaqI GGGAC 1 cut(s) 1728
FatI CATG 9 cut(s) 61, 203, 489, 837, 1446, 1462, 1554, 1803, 2024
Fnu4HI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
FokI GGATG 7 cut(s) 419, 559, 767, 1068, 1505, 1649, 1704
Fsp4HI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
FspBI CTAG 2 cut(s) 821, 1065
GlaI GCGC 3 cut(s) 378, 1368, 1606
GluI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
GsaI CCCAGC 2 cut(s) 229, 521
GsuI CTGGAG 2 cut(s) 423, 449
HaeII RGCGCY 1 cut(s) 1370
HaeIII GGCC 3 cut(s) 850, 1374, 1482
HapII CCGG 4 cut(s) 530, 1710, 2016, 2062
HgaI GACGC 3 cut(s) 478, 1424, 1636
HhaI GCGC 3 cut(s) 379, 1369, 1607
Hin1II CATG 9 cut(s) 65, 207, 493, 841, 1450, 1466, 1558, 1807, 2028
Hin6I GCGC 3 cut(s) 377, 1367, 1605
HinP1I GCGC 3 cut(s) 377, 1367, 1605
HindIII AAGCTT 3 cut(s) 145, 308, 994
HinfI GANTC 7 cut(s) 65, 117, 284, 979, 1013, 1019, 1640
HpaII CCGG 4 cut(s) 530, 1710, 2016, 2062
HphI GGTGA 2 cut(s) 736, 1463
Hpy166II GTNNAC 2 cut(s) 524, 1740
Hpy8I GTNNAC 2 cut(s) 524, 1740
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 1 cut(s) 825
HpyF10VI GCNNNNNNNGC 4 cut(s) 1160, 1169, 2038, 2073
HpyF3I CTNAG 5 cut(s) 93, 608, 714, 755, 1023
HpySE526I ACGT 1 cut(s) 825
Hsp92II CATG 9 cut(s) 65, 207, 493, 841, 1450, 1466, 1558, 1807, 2028
HspAI GCGC 3 cut(s) 377, 1367, 1605
Kzo9I GATC 4 cut(s) 1280, 1727, 1852, 2043
LguI GCTCTTC 1 cut(s) 2039
LmnI GCTCC 5 cut(s) 259, 1187, 1364, 1698, 2010
Lsp1109I GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
LweI GCATC 3 cut(s) 1307, 1321, 1597
MaeI CTAG 2 cut(s) 821, 1065
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 8 cut(s) 292, 406, 742, 1320, 1535, 1628, 1880, 2048
MalI GATC 4 cut(s) 1282, 1729, 1854, 2045
MboI GATC 4 cut(s) 1280, 1727, 1852, 2043
MfeI CAATTG 2 cut(s) 279, 1051
MflI RGATCY 1 cut(s) 2043
MhlI GDGCHC 1 cut(s) 1192
MlyI GAGTC 2 cut(s) 111, 1022
MmeI TCCRAC 6 cut(s) 155, 579, 858, 996, 1099, 1298
Mph1103I ATGCAT 1 cut(s) 1300
MroXI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2085
MseI TTAA 7 cut(s) 306, 402, 894, 957, 1830, 1938, 2098
MslI CAYNNNNRTG 4 cut(s) 639, 842, 920, 1735
MspA1I CMGCKG 2 cut(s) 229, 1757
MspI CCGG 4 cut(s) 530, 1710, 2016, 2062
MspR9I CCNGG 5 cut(s) 366, 1380, 1711, 1809, 2017
MunI CAATTG 2 cut(s) 279, 1051
Mva1269I GAATGC 3 cut(s) 927, 1300, 1779
MvaI CCWGG 3 cut(s) 366, 1380, 1809
MvnI CGCG 3 cut(s) 115, 377, 379
MwoI GCNNNNNNNGC 4 cut(s) 1160, 1169, 2038, 2073
NciI CCSGG 2 cut(s) 1711, 2017
NdeII GATC 4 cut(s) 1280, 1727, 1852, 2043
NlaIII CATG 9 cut(s) 65, 207, 493, 841, 1450, 1466, 1558, 1807, 2028
NlaIV GGNNCC 2 cut(s) 430, 849
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 3 cut(s) 406, 742, 1535
NsiI ATGCAT 1 cut(s) 1300
NspI RCATGY 2 cut(s) 493, 841
NspV TTCGAA 2 cut(s) 930, 1671
OliI CACNNNNGTG 1 cut(s) 1735
PaeR7I CTCGAG 2 cut(s) 1400, 1969
PagI TCATGA 1 cut(s) 61
PciI ACATGT 2 cut(s) 489, 837
PciSI GCTCTTC 1 cut(s) 2039
PctI GAATGC 3 cut(s) 927, 1300, 1779
PdmI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2085
PfeI GAWTC 5 cut(s) 65, 284, 979, 1019, 1640
PinAI ACCGGT 1 cut(s) 2061
PkrI GCNGC 5 cut(s) 231, 867, 1165, 1756, 1759
PleI GAGTC 2 cut(s) 111, 1021
PpsI GAGTC 2 cut(s) 111, 1021
PpuMI RGGWCCY 1 cut(s) 325
PscI ACATGT 2 cut(s) 489, 837
PshBI ATTAAT 1 cut(s) 1938
Psp5II RGGWCCY 1 cut(s) 325
Psp6I CCWGG 3 cut(s) 364, 1378, 1807
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 2 cut(s) 225, 517
PspGI CCWGG 3 cut(s) 364, 1378, 1807
PspN4I GGNNCC 2 cut(s) 430, 849
PspPI GGNCC 3 cut(s) 325, 710, 848
PspPPI RGGWCCY 1 cut(s) 325
PspXI VCTCGAGB 1 cut(s) 1400
PsuI RGATCY 1 cut(s) 2043
PvuII CAGCTG 2 cut(s) 229, 1757
RsaI GTAC 5 cut(s) 753, 1128, 1475, 1680, 2060
RsaNI GTAC 5 cut(s) 752, 1127, 1474, 1679, 2059
RseI CAYNNNNRTG 4 cut(s) 639, 842, 920, 1735
SapI GCTCTTC 1 cut(s) 2039
SaqAI TTAA 7 cut(s) 306, 402, 894, 957, 1830, 1938, 2098
SatI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
Sau3AI GATC 4 cut(s) 1280, 1727, 1852, 2043
Sau96I GGNCC 3 cut(s) 325, 710, 848
SchI GAGTC 2 cut(s) 111, 1022
ScrFI CCNGG 5 cut(s) 366, 1380, 1711, 1809, 2017
SduI GDGCHC 1 cut(s) 1192
SfaNI GCATC 3 cut(s) 1307, 1321, 1597
Sfr274I CTCGAG 2 cut(s) 1400, 1969
SfuI TTCGAA 2 cut(s) 930, 1671
SinI GGWCC 2 cut(s) 325, 710
SlaI CTCGAG 2 cut(s) 1400, 1969
SmiMI CAYNNNNRTG 4 cut(s) 639, 842, 920, 1735
SmlI CTYRAG 4 cut(s) 1010, 1400, 1409, 1969
SmoI CTYRAG 4 cut(s) 1010, 1400, 1409, 1969
SpeI ACTAGT 1 cut(s) 1064
SsiI CCGC 3 cut(s) 197, 1085, 2041
SspI AATATT 2 cut(s) 1659, 2086
SspMI CTAG 2 cut(s) 821, 1065
StyD4I CCNGG 5 cut(s) 364, 1378, 1709, 1807, 2015
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 1 cut(s) 828
TaqII GACCGA 3 cut(s) 762, 1149, 1205
TatI WGTACW 2 cut(s) 751, 1473
TfiI GAWTC 5 cut(s) 65, 284, 979, 1019, 1640
Tru1I TTAA 7 cut(s) 306, 402, 894, 957, 1830, 1938, 2098
Tru9I TTAA 7 cut(s) 306, 402, 894, 957, 1830, 1938, 2098
TscAI CASTG 3 cut(s) 1549, 1622, 1994
TseFI GTSAC 3 cut(s) 406, 742, 1535
TseI GCWGC 5 cut(s) 229, 865, 1163, 1754, 1757
Tsp45I GTSAC 3 cut(s) 406, 742, 1535
TspRI CASTG 3 cut(s) 1549, 1622, 1994
VpaK11BI GGWCC 2 cut(s) 325, 710
VspI ATTAAT 1 cut(s) 1938
XapI RAATTY 7 cut(s) 184, 415, 767, 911, 934, 1548, 1858
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 493, 841
XcmI CCANNNNNNNNNTGG 2 cut(s) 841, 1490
XhoI CTCGAG 2 cut(s) 1400, 1969
XmnI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2085
XspI CTAG 2 cut(s) 821, 1065
Zsp2I ATGCAT 1 cut(s) 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.