Rroxscaffold_4G00319110
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001328
Physical Location & Seq
Forward (+)
46607930 .. 46612730
4801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319110.1

Sequence Viewer

Length: 1242 bp
ATGGCAAAACATATTTACCCTTCACATCTTCTCTTCTTCCTGTTGGTGGTTTTACTTTGTTTCCATCACTCACAGCAATCCCAGTCCCAGTCCCAGTCCCAACAATGGCAGAGCCTTGTGGAAATCCAGAAGCTTCTAAACTACCCCTCAGCTTTACGTAGCTTCAAGTACACCAGAAGAGGCAGAGGCCTGTGCAGCATTGAACCAACCCCATCTTTGACTCTAGCCTGTTATCAAGGCAACATAACCCAGCTTCACATCACTGGCAACAATGAGTTTCCTCCTCTGCCTAGTGATTTCTCATCTCACTCTTTCTTTGCAAGCATTGCTCAGTTACCAAGCTTGAAAGTTGTTTCTTTGACTTGTCTTGGTTTATGGGGTCCTATCCCTCCAAGTATTGCTCAGTTATCTTCTCTTGAGATACTCAATGTCAGTACAAACTACTTGAGTGGAACCATCCCTCTTCAAATTTCATCCCTCAGAAACCTTCAGACACTCATACTTGACCACAACAAGTTTACTGGTCAAGTCCCAGCTTGGTTAAGCTCACTGCCAGTCTTGTCTGTTTTGAGTTTCAAGAACAATTGGCTTAATGGGTCTCTTCCATATTCTTTCTCATCTTTGCAGACACTCAGAGTTGTCTCTCTCTCCCACAACTACTTGTCCGGGGAAGTCCCTGGTCTTACCAACCTGACAAACTTGCAAGTTCTTGATTTGGAAGACAACTATTTCGGGCCACATTTTCCGAGTTTGCCTAACAAGTTAGTCACACTTGTGCTGAGGAAGAACAAGTTTCGCTTGGGAATCCAGACCACATTAGACTCCTCCTGGTTTCAGCTTCAAAAGCTGGACATCTCATTGAATAGATTTGTAGGACCATTTATGCCATCATTGTTGTCTCTGCCTTCCATTAATTATCTCGACATTGGTGGCAACAAGTTCACTGGAGTACTCTCCAAGAACATGTCTTGCAATGCTGAGCTTGCTTTTGTCAATTTATCCTCAAATCTCTTGACAGGGGAGTTGCCCGATTGTGTTAAAAAGACCAGGATTTCTCTGTATACTCAGAATTGCTTTGCAAATGAAGATCAGGATCAGCAACCTTCTAACTTGTGCCACAATGAAGCTCTGGCTGTGCAAATACCGCCGAGTAAGGAAGAGCATAAGAGACATTATGCTAAGCATGTAATTGCATCAAGTGCAGTGGGGGTATTGTTGGTGCAATTGCACTTGTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

413

Amino Acids

45.97

Weight (kDa)

8.83

Isoelectric Point (pI)

59.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 106 - 319 1.4e-11 Leucine-rich repeat region
LRR_8 PF13855 161 - 221 1.8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1061
AciI CCGC 1 cut(s) 1145
AclWI GGATC 1 cut(s) 1101
AcsI RAATTY 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 3 cut(s) 170, 436, 951
AfiI CCNNNNNNNGG 2 cut(s) 46, 105
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 7 cut(s) 166, 203, 346, 467, 577, 842, 862
AjnI CCWGG 3 cut(s) 676, 827, 1046
AleI CACNNNNGTG 1 cut(s) 773
Alw26I GTCTC 4 cut(s) 603, 646, 903, 1162
AlwI GGATC 1 cut(s) 1101
AoxI GGCC 2 cut(s) 187, 734
ApeKI GCWGC 1 cut(s) 195
ApoI RAATTY 1 cut(s) 468
ArsI GACNNNNNNTTYG 2 cut(s) 713, 745
AseI ATTAAT 1 cut(s) 912
AspS9I GGNCC 3 cut(s) 380, 734, 875
AsuC2I CCSGG 1 cut(s) 667
AvaII GGWCC 2 cut(s) 380, 875
BbsI GAAGAC 1 cut(s) 726
BbvCI CCTCAGC 2 cut(s) 148, 779
BbvI GCAGC 1 cut(s) 207
BccI CCATC 4 cut(s) 72, 220, 464, 895
BciT130I CCWGG 3 cut(s) 678, 829, 1048
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 4 cut(s) 603, 646, 903, 1162
BfaI CTAG 2 cut(s) 224, 291
BisI GCNGC 1 cut(s) 196
BlpI GCTNAGC 2 cut(s) 978, 1179
BlsI GCNGC 1 cut(s) 197
BmcAI AGTACT 1 cut(s) 951
Bme1390I CCNGG 4 cut(s) 667, 678, 829, 1048
Bme18I GGWCC 2 cut(s) 380, 875
BmgT120I GGNCC 3 cut(s) 380, 734, 875
BmiI GGNNCC 2 cut(s) 381, 454
BmrFI CCNGG 4 cut(s) 667, 678, 829, 1048
BmrI ACTGGG 3 cut(s) 76, 82, 88
BmsI GCATC 1 cut(s) 1202
BmuI ACTGGG 3 cut(s) 76, 82, 88
BpiI GAAGAC 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 966
Bpu10I CCTNAGC 2 cut(s) 148, 779
Bpu1102I GCTNAGC 2 cut(s) 978, 1179
BpuEI CTTGAG 2 cut(s) 437, 466
BpuMI CCSGG 1 cut(s) 667
BsaAI YACGTR 1 cut(s) 158
BsaBI GATNNNNATC 1 cut(s) 1092
BsaI GGTCTC 1 cut(s) 603
BsaJI CCNNGG 2 cut(s) 666, 676
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 105
Bse1I ACTGG 7 cut(s) 82, 88, 94, 268, 526, 554, 949
Bse3DI GCAATG 2 cut(s) 324, 979
Bse8I GATNNNNATC 1 cut(s) 1092
BseBI CCWGG 3 cut(s) 678, 829, 1048
BseDI CCNNGG 2 cut(s) 666, 676
BseGI GGATG 2 cut(s) 456, 473
BseJI GATNNNNATC 1 cut(s) 1092
BseLI CCNNNNNNNGG 2 cut(s) 46, 105
BseMI GCAATG 2 cut(s) 324, 979
BseMII CTCAG 8 cut(s) 162, 344, 416, 493, 646, 770, 969, 1079
BseNI ACTGG 7 cut(s) 82, 88, 94, 268, 526, 554, 949
BseRI GAGGAG 2 cut(s) 273, 814
BseXI GCAGC 1 cut(s) 207
BseYI CCCAGC 2 cut(s) 249, 532
BsgI GTGCAG 2 cut(s) 214, 1221
BshFI GGCC 2 cut(s) 189, 736
BsiSI CCGG 1 cut(s) 666
BslFI GGGAC 5 cut(s) 70, 76, 82, 515, 659
BslI CCNNNNNNNGG 2 cut(s) 46, 105
BsmAI GTCTC 4 cut(s) 603, 646, 903, 1162
BsmFI GGGAC 5 cut(s) 70, 76, 82, 515, 659
BsnI GGCC 2 cut(s) 189, 736
Bso31I GGTCTC 1 cut(s) 603
Bsp143I GATC 2 cut(s) 1087, 1093
Bsp1720I GCTNAGC 2 cut(s) 978, 1179
BspACI CCGC 1 cut(s) 1145
BspANI GGCC 2 cut(s) 189, 736
BspCNI CTCAG 8 cut(s) 161, 343, 415, 492, 645, 771, 970, 1078
BspLI GGNNCC 2 cut(s) 381, 454
BspPI GGATC 1 cut(s) 1101
BspQI GCTCTTC 1 cut(s) 1152
BspTNI GGTCTC 1 cut(s) 603
BsrDI GCAATG 2 cut(s) 324, 979
BsrI ACTGG 7 cut(s) 82, 88, 94, 268, 526, 554, 949
BssECI CCNNGG 2 cut(s) 666, 676
BssMI GATC 2 cut(s) 1087, 1093
BssNAI GTATAC 1 cut(s) 1062
Bst1107I GTATAC 1 cut(s) 1062
Bst2UI CCWGG 3 cut(s) 678, 829, 1048
Bst6I CTCTTC 5 cut(s) 38, 172, 468, 606, 1152
BstAPI GCANNNNNTGC 2 cut(s) 326, 1199
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 2 cut(s) 322, 984
BstDEI CTNAG 9 cut(s) 148, 330, 402, 479, 632, 779, 978, 1065, 1179
BstF5I GGATG 2 cut(s) 456, 473
BstKTI GATC 2 cut(s) 1090, 1096
BstMAI GTCTC 4 cut(s) 603, 646, 903, 1162
BstMBI GATC 2 cut(s) 1087, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 195, 326, 844, 983, 1144, 1199
BstNI CCWGG 3 cut(s) 678, 829, 1048
BstNSI RCATGY 2 cut(s) 967, 1187
BstSCI CCNGG 4 cut(s) 665, 676, 827, 1046
BstSNI TACGTA 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 207
BstV2I GAAGAC 1 cut(s) 726
BstZ17I GTATAC 1 cut(s) 1062
BsuRI GGCC 2 cut(s) 189, 736
BtsCI GGATG 2 cut(s) 456, 473
BtsI GCAGTG 2 cut(s) 548, 1209
BtsIMutI CAGTG 4 cut(s) 261, 548, 942, 1209
Cac8I GCNNGC 2 cut(s) 322, 984
Cfr13I GGNCC 3 cut(s) 380, 734, 875
Csp6I GTAC 3 cut(s) 169, 435, 950
CspCI CAANNNNNGTGG 2 cut(s) 1185, 1220
CviAII CATG 2 cut(s) 964, 1184
CviQI GTAC 3 cut(s) 169, 435, 950
DdeI CTNAG 9 cut(s) 148, 330, 402, 479, 632, 779, 978, 1065, 1179
DpnI GATC 2 cut(s) 1089, 1095
DpnII GATC 2 cut(s) 1087, 1093
Eam1104I CTCTTC 5 cut(s) 38, 172, 468, 606, 1152
EarI CTCTTC 5 cut(s) 38, 172, 468, 606, 1152
Eco105I TACGTA 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 603
Eco47I GGWCC 2 cut(s) 380, 875
Eco57I CTGAAG 1 cut(s) 473
EcoO109I RGGNCCY 1 cut(s) 380
EcoRII CCWGG 3 cut(s) 676, 827, 1046
FaeI CATG 2 cut(s) 967, 1187
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FaqI GGGAC 5 cut(s) 70, 76, 82, 515, 659
FatI CATG 2 cut(s) 963, 1183
FblI GTMKAC 1 cut(s) 1061
Fnu4HI GCNGC 1 cut(s) 196
FokI GGATG 2 cut(s) 443, 460
Fsp4HI GCNGC 1 cut(s) 196
FspBI CTAG 2 cut(s) 224, 291
GluI GCNGC 1 cut(s) 196
GsaI CCCAGC 2 cut(s) 253, 536
GsuI CTGGAG 1 cut(s) 966
HaeIII GGCC 2 cut(s) 189, 736
HapII CCGG 1 cut(s) 666
Hin1II CATG 2 cut(s) 967, 1187
HindIII AAGCTT 2 cut(s) 131, 340
HinfI GANTC 3 cut(s) 220, 804, 821
HpaII CCGG 1 cut(s) 666
Hpy166II GTNNAC 4 cut(s) 171, 519, 942, 1062
Hpy188I TCNGA 5 cut(s) 482, 492, 635, 747, 1068
Hpy188III TCNNGA 8 cut(s) 127, 416, 577, 710, 808, 920, 1012, 1091
Hpy8I GTNNAC 4 cut(s) 171, 519, 942, 1062
HpyAV CCTTC 4 cut(s) 30, 497, 915, 1113
HpyCH4IV ACGT 1 cut(s) 157
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 326, 844, 983, 1144, 1199
HpyF3I CTNAG 9 cut(s) 148, 330, 402, 479, 632, 779, 978, 1065, 1179
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 2 cut(s) 967, 1187
Kzo9I GATC 2 cut(s) 1087, 1093
LguI GCTCTTC 1 cut(s) 1152
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 1 cut(s) 1202
MaeI CTAG 2 cut(s) 224, 291
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 2 cut(s) 333, 766
MalI GATC 2 cut(s) 1089, 1095
MboI GATC 2 cut(s) 1087, 1093
MfeI CAATTG 2 cut(s) 583, 1223
MluCI AATT 7 cut(s) 468, 583, 913, 994, 1069, 1188, 1223
MlyI GAGTC 2 cut(s) 214, 815
MseI TTAA 4 cut(s) 542, 591, 912, 1038
MslI CAYNNNNRTG 1 cut(s) 773
MspI CCGG 1 cut(s) 666
MspR9I CCNGG 4 cut(s) 667, 678, 829, 1048
MunI CAATTG 2 cut(s) 583, 1223
MvaI CCWGG 3 cut(s) 678, 829, 1048
MwoI GCNNNNNNNGC 6 cut(s) 195, 326, 844, 983, 1144, 1199
NciI CCSGG 1 cut(s) 667
NdeII GATC 2 cut(s) 1087, 1093
NlaIII CATG 2 cut(s) 967, 1187
NlaIV GGNNCC 2 cut(s) 381, 454
NmeAIII GCCGAG 1 cut(s) 1173
NmuCI GTSAC 1 cut(s) 766
NspI RCATGY 2 cut(s) 967, 1187
OliI CACNNNNGTG 1 cut(s) 773
PceI AGGCCT 1 cut(s) 189
PciI ACATGT 1 cut(s) 963
PciSI GCTCTTC 1 cut(s) 1152
PfeI GAWTC 1 cut(s) 804
PkrI GCNGC 1 cut(s) 197
PleI GAGTC 2 cut(s) 214, 815
PpsI GAGTC 2 cut(s) 214, 815
Ppu21I YACGTR 1 cut(s) 158
PpuMI RGGWCCY 1 cut(s) 380
PscI ACATGT 1 cut(s) 963
PshBI ATTAAT 1 cut(s) 912
Psp5II RGGWCCY 1 cut(s) 380
Psp6I CCWGG 3 cut(s) 676, 827, 1046
PspFI CCCAGC 2 cut(s) 249, 532
PspGI CCWGG 3 cut(s) 676, 827, 1046
PspN4I GGNNCC 2 cut(s) 381, 454
PspPI GGNCC 3 cut(s) 380, 734, 875
PspPPI RGGWCCY 1 cut(s) 380
RsaI GTAC 3 cut(s) 170, 436, 951
RsaNI GTAC 3 cut(s) 169, 435, 950
RseI CAYNNNNRTG 1 cut(s) 773
SapI GCTCTTC 1 cut(s) 1152
SaqAI TTAA 4 cut(s) 542, 591, 912, 1038
SatI GCNGC 1 cut(s) 196
Sau3AI GATC 2 cut(s) 1087, 1093
Sau96I GGNCC 3 cut(s) 380, 734, 875
ScaI AGTACT 1 cut(s) 951
SchI GAGTC 2 cut(s) 214, 815
ScrFI CCNGG 4 cut(s) 667, 678, 829, 1048
SfaNI GCATC 1 cut(s) 1202
SinI GGWCC 2 cut(s) 380, 875
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 2 cut(s) 416, 445
SmoI CTYRAG 2 cut(s) 416, 445
SnaBI TACGTA 1 cut(s) 158
Sse9I AATT 7 cut(s) 468, 583, 913, 994, 1069, 1188, 1223
SseBI AGGCCT 1 cut(s) 189
SsiI CCGC 1 cut(s) 1145
SspMI CTAG 2 cut(s) 224, 291
StuI AGGCCT 1 cut(s) 189
StyD4I CCNGG 4 cut(s) 665, 676, 827, 1046
TaiI ACGT 1 cut(s) 160
TaqI TCGA 1 cut(s) 921
TasI AATT 7 cut(s) 468, 583, 913, 994, 1069, 1188, 1223
TatI WGTACW 3 cut(s) 168, 434, 949
TfiI GAWTC 1 cut(s) 804
Tru1I TTAA 4 cut(s) 542, 591, 912, 1038
Tru9I TTAA 4 cut(s) 542, 591, 912, 1038
TscAI CASTG 4 cut(s) 268, 555, 949, 1209
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 1 cut(s) 195
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 3 cut(s) 462, 1098, 1137
TspRI CASTG 4 cut(s) 268, 555, 949, 1209
VpaK11BI GGWCC 2 cut(s) 380, 875
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 1 cut(s) 468
XceI RCATGY 2 cut(s) 967, 1187
XmiI GTMKAC 1 cut(s) 1061
XspI CTAG 2 cut(s) 224, 291
ZrmI AGTACT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.