Rorug03G0281400
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001328
Physical Location & Seq
Reverse (-)
28069221 .. 28074496
5276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0281400.1

Sequence Viewer

Length: 945 bp
ATGGAAAAGCAATCTCATACTGTTGCATCTCCTGGAATAAGGAGAGGTCCATGGACTCCTGAGGAGGACAAGAAGCTCTTTGCTTTCGTTCAACAACATGGCCATGGAAGCTGGCGTTCCGTCCCCCAGAAAGCTGGTCTCCAAAGATGTGGAAAGAGCTGCAGGCTAAGATGGAGAAACTATCTAAACCCTGATATTAAGAGAGGAAATTTCAGTTTGCAGGAAGACCAGACCATCATTCTACTTCATGCACTTTTGGGCAACAGGTGGTCCGCCATAGCTGCACAGTTACCAAAGAGAACAGACAATGAGATAAAGAACTACTGGAACACACATCTAAAGAAGAGGTTAGCCAAGATAGGGTTTGATCCTGTAACCCACAAGCCCAAGACCGCCATCCTCGCCTCTGCTGATGGCGGAGACCCGAAGAACCTCTCAAACCTCAGCCACATAGCTCAGTGGGAAACTGCTCGGCTTCAAGCAGAAGCTAGATTCTCCAGACAGTCAAAACTACTCATCAGTCATGACCAACCTCCTAGGTTTGGACCCGTGCCTGTACCACAATGCCTTGACATGAATGAAAGAGCACGAGAAATTCGATTACTGTTAAAGTCAATATTAGCTGCAGACAGTGGTGCCCAATTAGGTGGTGGTATTGGTAGTACAGCCACCCACGTTGGGCAGGAGGTTGGTTCATTTGGTAAAGTTGATGTTGAGGGCTTGCTTACTAATTGTGATCAGTTTGAGGCTCCAAGAACCAGTACATCAGTTTTGGTTTTTGATGAAGTTTTTGGGGAGTCCGGTACTAATAATGACTATGGTGAATTGTTGGAGGCATTACTACAGCACAACCATAACAGTACTGGATTTGAAGTTGGGGATGCTTGGAGTTTGGAACAACTTGAAACAGTAATGATGAGTGAGCCGGGAACAACGTACATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

314

Amino Acids

34.87

Weight (kDa)

7.76

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 15 - 62 2.3e-18 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 18 - 78 3.1e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 68 - 113 1.6e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 71 - 123 4.2e-09 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 635
AciI CCGC 3 cut(s) 273, 393, 417
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 2 cut(s) 208, 594
AfaI GTAC 6 cut(s) 558, 664, 763, 805, 862, 938
AfiI CCNNNNNNNGG 3 cut(s) 360, 542, 678
AgsI TTSAA 4 cut(s) 92, 479, 872, 905
AjnI CCWGG 1 cut(s) 31
AluBI AGCT 8 cut(s) 76, 111, 134, 159, 281, 455, 488, 623
AluI AGCT 8 cut(s) 76, 111, 134, 159, 281, 455, 488, 623
Alw21I GWGCWC 1 cut(s) 589
Alw26I GTCTC 2 cut(s) 143, 414
AlwI GGATC 1 cut(s) 362
AoxI GGCC 1 cut(s) 100
ApeKI GCWGC 3 cut(s) 159, 281, 623
ApoI RAATTY 2 cut(s) 208, 594
AspA2I CCTAGG 1 cut(s) 536
AspS9I GGNCC 3 cut(s) 47, 270, 545
AsuC2I CCSGG 1 cut(s) 927
AsuHPI GGTGA 1 cut(s) 833
AvaII GGWCC 3 cut(s) 47, 270, 545
AvrII CCTAGG 1 cut(s) 536
AxyI CCTNAGG 1 cut(s) 60
BaeGI GKGCMC 1 cut(s) 640
BalI TGGCCA 1 cut(s) 102
BanI GGYRCC 1 cut(s) 635
BauI CACGAG 1 cut(s) 588
BbsI GAAGAC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 589
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 146, 268, 610
BccI CCATC 4 cut(s) 165, 242, 404, 407
BciT130I CCWGG 1 cut(s) 33
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 1 cut(s) 927
BcoDI GTCTC 2 cut(s) 143, 414
BfaI CTAG 2 cut(s) 489, 537
BfmI CTRYAG 3 cut(s) 160, 624, 842
BisI GCNGC 3 cut(s) 160, 282, 624
BlnI CCTAGG 1 cut(s) 536
BlsI GCNGC 3 cut(s) 161, 283, 625
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 2 cut(s) 33, 927
Bme18I GGWCC 3 cut(s) 47, 270, 545
BmgT120I GGNCC 3 cut(s) 47, 270, 545
BmiI GGNNCC 3 cut(s) 547, 637, 750
BmrFI CCNGG 2 cut(s) 33, 927
BmsI GCATC 2 cut(s) 35, 871
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 481
Bpu10I CCTNAGC 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 927
BsaI GGTCTC 2 cut(s) 143, 414
BsaJI CCNNGG 3 cut(s) 50, 103, 536
BsaWI WCCGGW 1 cut(s) 800
Bsc4I CCNNNNNNNGG 3 cut(s) 360, 542, 678
Bse1I ACTGG 3 cut(s) 329, 759, 868
Bse21I CCTNAGG 1 cut(s) 60
BseBI CCWGG 1 cut(s) 33
BseDI CCNNGG 3 cut(s) 50, 103, 536
BseGI GGATG 2 cut(s) 396, 886
BseLI CCNNNNNNNGG 3 cut(s) 360, 542, 678
BseMII CTCAG 3 cut(s) 51, 457, 470
BseNI ACTGG 3 cut(s) 329, 759, 868
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 640
BseXI GCAGC 3 cut(s) 146, 268, 610
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 1 cut(s) 102
BshNI GGYRCC 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 589
BsiSI CCGG 2 cut(s) 801, 926
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 3 cut(s) 360, 542, 678
BsmAI GTCTC 2 cut(s) 143, 414
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 1 cut(s) 102
Bso31I GGTCTC 2 cut(s) 143, 414
Bsp1286I GDGCHC 2 cut(s) 589, 640
Bsp143I GATC 2 cut(s) 367, 736
Bsp19I CCATGG 2 cut(s) 50, 103
BspACI CCGC 3 cut(s) 273, 393, 417
BspANI GGCC 1 cut(s) 102
BspCNI CTCAG 3 cut(s) 52, 456, 469
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 3 cut(s) 547, 637, 750
BspMAI CTGCAG 2 cut(s) 164, 628
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 1 cut(s) 635
BspTNI GGTCTC 2 cut(s) 143, 414
BsrI ACTGG 3 cut(s) 329, 759, 868
BssECI CCNNGG 3 cut(s) 50, 103, 536
BssMI GATC 2 cut(s) 367, 736
BssSI CACGAG 1 cut(s) 588
BssT1I CCWWGG 3 cut(s) 50, 103, 536
Bst2BI CACGAG 1 cut(s) 588
Bst2UI CCWGG 1 cut(s) 33
Bst4CI ACNGT 7 cut(s) 22, 288, 504, 606, 632, 860, 910
Bst6I CTCTTC 1 cut(s) 338
BstC8I GCNNGC 3 cut(s) 113, 164, 722
BstDEI CTNAG 4 cut(s) 60, 167, 443, 456
BstDSI CCRYGG 2 cut(s) 50, 103
BstF5I GGATG 2 cut(s) 396, 886
BstKTI GATC 2 cut(s) 370, 739
BstMAI GTCTC 2 cut(s) 143, 414
BstMBI GATC 2 cut(s) 367, 736
BstMWI GCNNNNNNNGC 3 cut(s) 108, 281, 401
BstNI CCWGG 1 cut(s) 33
BstSCI CCNGG 2 cut(s) 31, 925
BstSFI CTRYAG 3 cut(s) 160, 624, 842
BstSLI GKGCMC 1 cut(s) 640
BstV1I GCAGC 3 cut(s) 146, 268, 610
BstV2I GAAGAC 1 cut(s) 231
BstXI CCANNNNNNTGG 3 cut(s) 134, 149, 647
Bsu36I CCTNAGG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 50, 103
BtsCI GGATG 2 cut(s) 396, 886
BtsIMutI CAGTG 2 cut(s) 464, 637
Cac8I GCNNGC 3 cut(s) 113, 164, 722
CciI TCATGA 1 cut(s) 523
Cfr13I GGNCC 3 cut(s) 47, 270, 545
Csp6I GTAC 6 cut(s) 557, 663, 762, 804, 861, 937
CspCI CAANNNNNGTGG 2 cut(s) 658, 693
CviAII CATG 6 cut(s) 51, 98, 104, 248, 524, 574
CviQI GTAC 6 cut(s) 557, 663, 762, 804, 861, 937
DdeI CTNAG 4 cut(s) 60, 167, 443, 456
DpnI GATC 2 cut(s) 369, 738
DpnII GATC 2 cut(s) 367, 736
EaeI YGGCCR 1 cut(s) 100
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
EciI GGCGGA 2 cut(s) 262, 432
Eco130I CCWWGG 3 cut(s) 50, 103, 536
Eco31I GGTCTC 2 cut(s) 143, 414
Eco47I GGWCC 3 cut(s) 47, 270, 545
Eco81I CCTNAGG 1 cut(s) 60
EcoRII CCWGG 1 cut(s) 31
EcoT14I CCWWGG 3 cut(s) 50, 103, 536
ErhI CCWWGG 3 cut(s) 50, 103, 536
FaeI CATG 6 cut(s) 54, 101, 107, 251, 527, 577
FalI AAGNNNNNCTT 2 cut(s) 62, 94
FaqI GGGAC 1 cut(s) 107
FatI CATG 6 cut(s) 50, 97, 103, 247, 523, 573
FbaI TGATCA 1 cut(s) 736
Fnu4HI GCNGC 3 cut(s) 160, 282, 624
FokI GGATG 2 cut(s) 383, 893
Fsp4HI GCNGC 3 cut(s) 160, 282, 624
FspBI CTAG 2 cut(s) 489, 537
GluI GCNGC 3 cut(s) 160, 282, 624
GsuI CTGGAG 1 cut(s) 481
HaeIII GGCC 1 cut(s) 102
HapII CCGG 2 cut(s) 801, 926
Hin1II CATG 6 cut(s) 54, 101, 107, 251, 527, 577
HinfI GANTC 3 cut(s) 55, 492, 797
HpaII CCGG 2 cut(s) 801, 926
HphI GGTGA 1 cut(s) 833
Hpy188III TCNNGA 3 cut(s) 59, 498, 524
HpyCH4III ACNGT 7 cut(s) 22, 288, 504, 606, 632, 860, 910
HpyCH4IV ACGT 2 cut(s) 675, 935
HpyCH4V TGCA 6 cut(s) 26, 162, 220, 251, 284, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 108, 281, 401
HpyF3I CTNAG 4 cut(s) 60, 167, 443, 456
HpySE526I ACGT 2 cut(s) 675, 935
Hsp92II CATG 6 cut(s) 54, 101, 107, 251, 527, 577
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 2 cut(s) 367, 736
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 3 cut(s) 146, 268, 610
LweI GCATC 2 cut(s) 35, 871
MaeI CTAG 2 cut(s) 489, 537
MaeII ACGT 2 cut(s) 675, 935
MaeIII GTNAC 2 cut(s) 288, 373
MalI GATC 2 cut(s) 369, 738
MboI GATC 2 cut(s) 367, 736
MboII GAAGA 3 cut(s) 236, 355, 439
MhlI GDGCHC 2 cut(s) 589, 640
MlsI TGGCCA 1 cut(s) 102
MluCI AATT 5 cut(s) 208, 594, 641, 730, 824
MluNI TGGCCA 1 cut(s) 102
MlyI GAGTC 2 cut(s) 49, 806
MmeI TCCRAC 1 cut(s) 810
Mox20I TGGCCA 1 cut(s) 102
MscI TGGCCA 1 cut(s) 102
MseI TTAA 2 cut(s) 198, 608
MslI CAYNNNNRTG 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 102
MspI CCGG 2 cut(s) 801, 926
MspR9I CCNGG 2 cut(s) 33, 927
MvaI CCWGG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 108, 281, 401
NciI CCSGG 1 cut(s) 927
NcoI CCATGG 2 cut(s) 50, 103
NdeII GATC 2 cut(s) 367, 736
NlaIII CATG 6 cut(s) 54, 101, 107, 251, 527, 577
NlaIV GGNNCC 3 cut(s) 547, 637, 750
NmeAIII GCCGAG 1 cut(s) 451
PagI TCATGA 1 cut(s) 523
PcsI WCGNNNNNNNCGW 1 cut(s) 595
PfeI GAWTC 1 cut(s) 492
PfoI TCCNGGA 1 cut(s) 31
PkrI GCNGC 3 cut(s) 161, 283, 625
PleI GAGTC 2 cut(s) 49, 805
PpsI GAGTC 2 cut(s) 49, 805
Psp6I CCWGG 1 cut(s) 31
PspGI CCWGG 1 cut(s) 31
PspN4I GGNNCC 3 cut(s) 547, 637, 750
PspPI GGNCC 3 cut(s) 47, 270, 545
PstI CTGCAG 2 cut(s) 164, 628
RsaI GTAC 6 cut(s) 558, 664, 763, 805, 862, 938
RsaNI GTAC 6 cut(s) 557, 663, 762, 804, 861, 937
RseI CAYNNNNRTG 1 cut(s) 102
SaqAI TTAA 2 cut(s) 198, 608
SatI GCNGC 3 cut(s) 160, 282, 624
Sau3AI GATC 2 cut(s) 367, 736
Sau96I GGNCC 3 cut(s) 47, 270, 545
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 2 cut(s) 49, 806
ScrFI CCNGG 2 cut(s) 33, 927
SduI GDGCHC 2 cut(s) 589, 640
SfaNI GCATC 2 cut(s) 35, 871
SfcI CTRYAG 3 cut(s) 160, 624, 842
SinI GGWCC 3 cut(s) 47, 270, 545
SmiMI CAYNNNNRTG 1 cut(s) 102
Sse9I AATT 5 cut(s) 208, 594, 641, 730, 824
SsiI CCGC 3 cut(s) 273, 393, 417
SspI AATATT 1 cut(s) 618
SspMI CTAG 2 cut(s) 489, 537
StyD4I CCNGG 2 cut(s) 31, 925
StyI CCWWGG 3 cut(s) 50, 103, 536
TaaI ACNGT 7 cut(s) 22, 288, 504, 606, 632, 860, 910
TaiI ACGT 2 cut(s) 678, 938
TaqI TCGA 1 cut(s) 598
TasI AATT 5 cut(s) 208, 594, 641, 730, 824
TatI WGTACW 3 cut(s) 662, 761, 860
TfiI GAWTC 1 cut(s) 492
Tru1I TTAA 2 cut(s) 198, 608
Tru9I TTAA 2 cut(s) 198, 608
TscAI CASTG 2 cut(s) 464, 637
TseI GCWGC 3 cut(s) 159, 281, 623
TspDTI ATGAA 5 cut(s) 236, 590, 594, 684, 798
TspGWI ACGGA 1 cut(s) 109
TspRI CASTG 2 cut(s) 464, 637
VpaK11BI GGWCC 3 cut(s) 47, 270, 545
XapI RAATTY 2 cut(s) 208, 594
XcmI CCANNNNNNNNNTGG 2 cut(s) 647, 860
XmaJI CCTAGG 1 cut(s) 536
XspI CTAG 2 cut(s) 489, 537
ZrmI AGTACT 1 cut(s) 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.