Rh3AG328000
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0001328
Physical Location & Seq
Reverse (-)
46632894 .. 46639362
6469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG328000.1

Sequence Viewer

Length: 1722 bp
ATGGCAAGAGTATCTCCCCGTTTGATGCATTTGGTTCTTGTTATCATTCTATTTTCAGTTAGTCATGCCAAAAATAATTCATCCTCCCAAGCTCTGACCCTTTTCAGAATTCAGCGACTTCTAAACCTTCCTGCTGTTTTGAGTAGATCATATAACTACACAGATTTTTGCAACATCGAACCAAGTTTGTCTTTGACCGTCATATGCTATGATGAAAAAATAACCCAGCTGCATATAATTGGTGAAAAAAATGCTCCTCTTTTGCCTAGAAACTTTTCAATTGATTTGTTCGTAACGACTCTTGTCAGACTTCCAAGCTTGAAGGTCCTCACATTGGTCTCTCTTGGTTTATGGGGACCTTTGCCTGGTAAAATCTCACAATTATCAGCACTGGAAATACTTAATGTGACTTCAAATTTCTTTTATGGTGTCATCCCTCAAGAGCTTTCATCACTATCAAGCCTCCAAACGCTCATACTTGATGATAACATGTTTTCTGGACCACTTCAAGATTGGATGAGTTTATTCCCTCTTCTTGCTGTTTTTAGTGTAAAGAAGAATTTGTTGAATGCTTCTTTGCCCAACTCATTCAGTGGTTTGGTAAGTCTTAGAGTTCTTGGGCTTTCTCATAACCACTTCTTTGGGGAAGTTCCTGATCTTAGTACTTTGACAAACCTTCAAGTTCTTGAATTGGAAGATAATGCTTTTGGTCCTCAGTTTCCCAAACTTGGCAAGAAGTTGGTGACCCTTGTACTGAGTAAGAATAAATTTAGGTCTGGCATCCCTGCTGAAGTGAGCTCTTATCATCAGCTAGAACGGCTTGATCTTTCTTTCAATATGTTTGTTGGGCCATTTCCATCATCATTGCTATCTCTACCTTCCATAACTTATCTGAATGTTTCACGAAACAAATTCACAGGAATGCTTTTTGAAAATTTGTCTTGCAATGCTGAGCTTCAGTATGTCGATTTGTCCTCAAATCTGTTGTCTGGAAGCTTACCTACATGTCTTCTGTCAGATTCTAAGGACAGTGTTGTCCTGTATGCTAGGAATTGTTTATCTACTGGAAATCAAAATCAGCATCGACTTCCATTTTGCCGCAACGAAGCATTAGCTGTTGGAATCATCCCTGACAGAAGCAAGCAAAAAAGAGCTTCTAAATCGATTCTGGCATTAGGTATAATTGGAGGAATTTTCGGAGGGGTTGTACTTGTCGGCTTAATTTTCTTTGTATATAGAAGAATGAATACCAACAAGACAATGAAGAAGTCTCCAGCAAGATCAATAACAGAAAATGCATCAACTGGATATAGCTCAAAGTTATTATCAGATGCAAGATATATATCACAAACAATGAAGCTGGGAGCGCTTGGCCTACCAAGCTATCGAACGTTTTCGCTGGATGAACTTGAGGAGGCGACAAATAATTTTGATACATCTACGTTCATGGGTGAAGGCTCGCATGGACAGATGTACAGAGGCCAGTTAAGGGATGGTTCTTTTGTTGCCATCAGATGCTTAAAAATGAAAACAAGTCACAGTACTCAAAATTTCATGCACCATATAGAGCTCATTTTGAAACTAAGGCATCGTCATTTGGTCAGTGCTCTTGGACACTGCTTTGAGTGTTACTTGGATGATTCTAGTGTCAGCAGAATATTTCTTGTTTTCGAATATGTACCAAATGGCACACTAAGAAGCTGGATCTCTGGTAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

573

Amino Acids

63.69

Weight (kDa)

9.26

Isoelectric Point (pI)

45.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 480 - 570 1.2e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 481 - 569 1.7e-09 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1034
AciI CCGC 1 cut(s) 1099
AclI AACGTT 1 cut(s) 1391
AclWI GGATC 1 cut(s) 1712
AcsI RAATTY 8 cut(s) 108, 415, 559, 767, 911, 934, 1191, 1549
AcuI CTGAAG 2 cut(s) 810, 941
AfaI GTAC 6 cut(s) 664, 753, 1209, 1475, 1543, 1680
AfeI AGCGCT 1 cut(s) 1368
AfiI CCNNNNNNNGG 4 cut(s) 334, 365, 728, 1489
AflIII ACRYGT 2 cut(s) 489, 1004
AjnI CCWGG 1 cut(s) 364
Alw21I GWGCWC 3 cut(s) 800, 1572, 1609
Alw26I GTCTC 2 cut(s) 343, 1275
AlwI GGATC 1 cut(s) 1712
Aor51HI AGCGCT 1 cut(s) 1368
AoxI GGCC 3 cut(s) 848, 1372, 1480
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 8 cut(s) 108, 415, 559, 767, 911, 934, 1191, 1549
ArsI GACNNNNNNTTYG 4 cut(s) 971, 1003, 1077, 1109
Asp700I GAANNNNTTC 2 cut(s) 274, 1393
AspLEI GCGC 1 cut(s) 1369
AspS9I GGNCC 5 cut(s) 325, 356, 500, 710, 848
AsuHPI GGTGA 3 cut(s) 254, 754, 1463
AsuII TTCGAA 1 cut(s) 1671
AvaII GGWCC 4 cut(s) 325, 356, 500, 710
BanII GRGCYC 2 cut(s) 800, 1572
BarI GAAGNNNNNNTAC 4 cut(s) 985, 1017, 1231, 1263
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 3 cut(s) 800, 1572, 1609
BbvI GCAGC 1 cut(s) 216
BccI CCATC 3 cut(s) 865, 1487, 1517
BceAI ACGGC 1 cut(s) 833
BciT130I CCWGG 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 343, 1275
BfaI CTAG 4 cut(s) 267, 812, 1047, 1644
BfoI RGCGCY 1 cut(s) 1370
BisI GCNGC 2 cut(s) 230, 1099
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 2 cut(s) 231, 1100
BmcAI AGTACT 2 cut(s) 664, 1543
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 4 cut(s) 325, 356, 500, 710
BmgT120I GGNCC 5 cut(s) 325, 356, 500, 710, 848
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 7 cut(s) 15, 789, 1090, 1307, 1321, 1505, 1597
BoxI GACNNNNGTC 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 1001
BpmI CTGGAG 1 cut(s) 1257
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 1671
BpuEI CTTGAG 2 cut(s) 423, 1430
Bsa29I ATCGAT 1 cut(s) 1163
BsaBI GATNNNNATC 1 cut(s) 1342
BsaI GGTCTC 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 365, 728, 1489
Bse1I ACTGG 4 cut(s) 396, 1069, 1309, 1483
Bse3DI GCAATG 2 cut(s) 863, 952
Bse8I GATNNNNATC 1 cut(s) 1342
BseBI CCWGG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 1163
BseGI GGATG 8 cut(s) 80, 432, 522, 780, 1125, 1408, 1498, 1642
BseJI GATNNNNATC 1 cut(s) 1342
BseLI CCNNNNNNNGG 4 cut(s) 334, 365, 728, 1489
BseMI GCAATG 2 cut(s) 863, 952
BseMII CTCAG 3 cut(s) 728, 746, 942
BseNI ACTGG 4 cut(s) 396, 1069, 1309, 1483
BseRI GAGGAG 2 cut(s) 246, 1427
BseXI GCAGC 1 cut(s) 216
BseYI CCCAGC 2 cut(s) 225, 1360
BshFI GGCC 3 cut(s) 850, 1374, 1482
BshVI ATCGAT 1 cut(s) 1163
BsiHKAI GWGCWC 3 cut(s) 800, 1572, 1609
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 4 cut(s) 334, 365, 728, 1489
BsmAI GTCTC 2 cut(s) 343, 1275
BsmFI GGGAC 1 cut(s) 369
BsmI GAATGC 2 cut(s) 574, 927
BsnI GGCC 3 cut(s) 850, 1374, 1482
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 1 cut(s) 1671
Bsp1286I GDGCHC 3 cut(s) 800, 1572, 1609
Bsp1407I TGTACA 1 cut(s) 1473
Bsp143I GATC 5 cut(s) 146, 655, 823, 1280, 1704
Bsp1720I GCTNAGC 1 cut(s) 951
BspACI CCGC 1 cut(s) 1099
BspANI GGCC 3 cut(s) 850, 1374, 1482
BspCNI CTCAG 3 cut(s) 727, 747, 943
BspDI ATCGAT 1 cut(s) 1163
BspHI TCATGA 1 cut(s) 1718
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 1 cut(s) 1712
BspT104I TTCGAA 1 cut(s) 1671
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 2 cut(s) 863, 952
BsrGI TGTACA 1 cut(s) 1473
BsrI ACTGG 4 cut(s) 396, 1069, 1309, 1483
BssMI GATC 5 cut(s) 146, 655, 823, 1280, 1704
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 3 cut(s) 199, 1031, 1541
Bst6I CTCTTC 1 cut(s) 537
BstAUI TGTACA 1 cut(s) 1473
BstBI TTCGAA 1 cut(s) 1671
BstC8I GCNNGC 2 cut(s) 1142, 1460
BstDEI CTNAG 8 cut(s) 608, 659, 714, 755, 951, 1023, 1583, 1694
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 8 cut(s) 80, 432, 522, 780, 1125, 1408, 1498, 1642
BstH2I RGCGCY 1 cut(s) 1370
BstHHI GCGC 1 cut(s) 1369
BstKTI GATC 5 cut(s) 149, 658, 826, 1283, 1707
BstMAI GTCTC 2 cut(s) 343, 1275
BstMBI GATC 5 cut(s) 146, 655, 823, 1280, 1704
BstMWI GCNNNNNNNGC 3 cut(s) 817, 1366, 1380
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 2 cut(s) 493, 1008
BstPAI GACNNNNGTC 1 cut(s) 302
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 364
BstV1I GCAGC 1 cut(s) 216
BstV2I GAAGAC 1 cut(s) 1001
BstX2I RGATCY 1 cut(s) 1704
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 1 cut(s) 1704
Bsu15I ATCGAT 1 cut(s) 1163
BsuRI GGCC 3 cut(s) 850, 1374, 1482
BsuTUI ATCGAT 1 cut(s) 1163
BtsCI GGATG 8 cut(s) 80, 432, 522, 780, 1125, 1408, 1498, 1642
BtsI GCAGTG 1 cut(s) 1615
BtsIMutI CAGTG 5 cut(s) 389, 598, 1036, 1609, 1615
Cac8I GCNNGC 2 cut(s) 1142, 1460
CciI TCATGA 1 cut(s) 1718
CfoI GCGC 1 cut(s) 1369
Cfr13I GGNCC 5 cut(s) 325, 356, 500, 710, 848
ClaI ATCGAT 1 cut(s) 1163
Csp6I GTAC 6 cut(s) 663, 752, 1208, 1474, 1542, 1679
CviAII CATG 7 cut(s) 65, 490, 1005, 1447, 1463, 1555, 1719
CviQI GTAC 6 cut(s) 663, 752, 1208, 1474, 1542, 1679
DdeI CTNAG 8 cut(s) 608, 659, 714, 755, 951, 1023, 1583, 1694
DpnI GATC 5 cut(s) 148, 657, 825, 1282, 1706
DpnII GATC 5 cut(s) 146, 655, 823, 1280, 1704
DrdI GACNNNNNNGTC 1 cut(s) 1034
DseDI GACNNNNNNGTC 1 cut(s) 1034
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Ecl136II GAGCTC 2 cut(s) 798, 1570
Eco24I GRGCYC 2 cut(s) 800, 1572
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 4 cut(s) 325, 356, 500, 710
Eco47III AGCGCT 1 cut(s) 1368
Eco53kI GAGCTC 2 cut(s) 798, 1570
Eco57I CTGAAG 2 cut(s) 810, 941
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 2 cut(s) 798, 1570
EcoO109I RGGNCCY 2 cut(s) 325, 356
EcoO65I GGTNACC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 108
EcoRII CCWGG 1 cut(s) 364
EcoT22I ATGCAT 2 cut(s) 30, 1300
EcoT38I GRGCYC 2 cut(s) 800, 1572
FaeI CATG 7 cut(s) 68, 493, 1008, 1450, 1466, 1558, 1722
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FaqI GGGAC 1 cut(s) 369
FatI CATG 7 cut(s) 64, 489, 1004, 1446, 1462, 1554, 1718
FauNDI CATATG 1 cut(s) 203
Fnu4HI GCNGC 2 cut(s) 230, 1099
FokI GGATG 8 cut(s) 67, 419, 529, 767, 1112, 1415, 1505, 1649
FriOI GRGCYC 2 cut(s) 800, 1572
Fsp4HI GCNGC 2 cut(s) 230, 1099
FspBI CTAG 4 cut(s) 267, 812, 1047, 1644
GlaI GCGC 1 cut(s) 1368
GluI GCNGC 2 cut(s) 230, 1099
GsaI CCCAGC 2 cut(s) 229, 1364
GsuI CTGGAG 1 cut(s) 1257
HaeII RGCGCY 1 cut(s) 1370
HaeIII GGCC 3 cut(s) 850, 1374, 1482
HhaI GCGC 1 cut(s) 1369
Hin1II CATG 7 cut(s) 68, 493, 1008, 1450, 1466, 1558, 1722
Hin6I GCGC 1 cut(s) 1367
HinP1I GCGC 1 cut(s) 1367
HindIII AAGCTT 2 cut(s) 316, 994
HinfI GANTC 5 cut(s) 298, 1019, 1122, 1165, 1640
HphI GGTGA 3 cut(s) 254, 754, 1463
Hpy188I TCNGA 8 cut(s) 96, 107, 308, 894, 1018, 1199, 1330, 1514
Hpy188III TCNNGA 8 cut(s) 440, 498, 509, 653, 686, 903, 990, 1719
HpyAV CCTTC 5 cut(s) 137, 316, 686, 888, 1448
HpyCH4III ACNGT 3 cut(s) 199, 1031, 1541
HpyCH4IV ACGT 2 cut(s) 1391, 1442
HpyCH4V TGCA 7 cut(s) 28, 171, 232, 945, 1298, 1334, 1558
HpyF10VI GCNNNNNNNGC 3 cut(s) 817, 1366, 1380
HpyF3I CTNAG 8 cut(s) 608, 659, 714, 755, 951, 1023, 1583, 1694
HpySE526I ACGT 2 cut(s) 1391, 1442
Hsp92II CATG 7 cut(s) 68, 493, 1008, 1450, 1466, 1558, 1722
HspAI GCGC 1 cut(s) 1367
Kzo9I GATC 5 cut(s) 146, 655, 823, 1280, 1704
LmnI GCTCC 2 cut(s) 259, 1364
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 7 cut(s) 15, 789, 1090, 1307, 1321, 1505, 1597
MaeI CTAG 4 cut(s) 267, 812, 1047, 1644
MaeII ACGT 2 cut(s) 1391, 1442
MaeIII GTNAC 5 cut(s) 292, 406, 742, 1535, 1628
MalI GATC 5 cut(s) 148, 657, 825, 1282, 1706
MboI GATC 5 cut(s) 146, 655, 823, 1280, 1704
MboII GAAGA 6 cut(s) 524, 568, 707, 1001, 1251, 1276
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 1 cut(s) 1704
MhlI GDGCHC 3 cut(s) 800, 1572, 1609
MlyI GAGTC 1 cut(s) 292
MmeI TCCRAC 1 cut(s) 1099
Mph1103I ATGCAT 2 cut(s) 30, 1300
MroXI GAANNNNTTC 2 cut(s) 274, 1393
MseI TTAA 4 cut(s) 402, 1220, 1487, 1520
MslI CAYNNNNRTG 1 cut(s) 920
MspA1I CMGCKG 1 cut(s) 229
MspR9I CCNGG 1 cut(s) 366
MunI CAATTG 1 cut(s) 279
Mva1269I GAATGC 2 cut(s) 574, 927
MvaI CCWGG 1 cut(s) 366
MwoI GCNNNNNNNGC 3 cut(s) 817, 1366, 1380
NdeI CATATG 1 cut(s) 203
NdeII GATC 5 cut(s) 146, 655, 823, 1280, 1704
NlaIII CATG 7 cut(s) 68, 493, 1008, 1450, 1466, 1558, 1722
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 3 cut(s) 406, 742, 1535
NsiI ATGCAT 2 cut(s) 30, 1300
NspI RCATGY 2 cut(s) 493, 1008
NspV TTCGAA 1 cut(s) 1671
PagI TCATGA 1 cut(s) 1718
PciI ACATGT 2 cut(s) 489, 1004
PctI GAATGC 2 cut(s) 574, 927
PdmI GAANNNNTTC 2 cut(s) 274, 1393
PfeI GAWTC 4 cut(s) 1019, 1122, 1165, 1640
PkrI GCNGC 2 cut(s) 231, 1100
PleI GAGTC 1 cut(s) 292
PpsI GAGTC 1 cut(s) 292
PpuMI RGGWCCY 2 cut(s) 325, 356
PscI ACATGT 2 cut(s) 489, 1004
PshAI GACNNNNGTC 1 cut(s) 302
Psp124BI GAGCTC 2 cut(s) 800, 1572
Psp1406I AACGTT 1 cut(s) 1391
Psp5II RGGWCCY 2 cut(s) 325, 356
Psp6I CCWGG 1 cut(s) 364
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 2 cut(s) 225, 1360
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 5 cut(s) 325, 356, 500, 710, 848
PspPPI RGGWCCY 2 cut(s) 325, 356
PsuI RGATCY 1 cut(s) 1704
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 6 cut(s) 664, 753, 1209, 1475, 1543, 1680
RsaNI GTAC 6 cut(s) 663, 752, 1208, 1474, 1542, 1679
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 2 cut(s) 800, 1572
SaqAI TTAA 4 cut(s) 402, 1220, 1487, 1520
SatI GCNGC 2 cut(s) 230, 1099
Sau3AI GATC 5 cut(s) 146, 655, 823, 1280, 1704
Sau96I GGNCC 5 cut(s) 325, 356, 500, 710, 848
ScaI AGTACT 2 cut(s) 664, 1543
SchI GAGTC 1 cut(s) 292
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 3 cut(s) 800, 1572, 1609
SfaNI GCATC 7 cut(s) 15, 789, 1090, 1307, 1321, 1505, 1597
SfuI TTCGAA 1 cut(s) 1671
SinI GGWCC 4 cut(s) 325, 356, 500, 710
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 2 cut(s) 438, 1409
SmoI CTYRAG 2 cut(s) 438, 1409
SsiI CCGC 1 cut(s) 1099
SspI AATATT 1 cut(s) 1659
SspMI CTAG 4 cut(s) 267, 812, 1047, 1644
SstI GAGCTC 2 cut(s) 800, 1572
StyD4I CCNGG 1 cut(s) 364
TaaI ACNGT 3 cut(s) 199, 1031, 1541
TaiI ACGT 2 cut(s) 1394, 1445
TaqI TCGA 6 cut(s) 177, 966, 1084, 1163, 1387, 1671
TatI WGTACW 5 cut(s) 662, 751, 1207, 1473, 1541
TauI GCSGC 1 cut(s) 1101
TfiI GAWTC 4 cut(s) 1019, 1122, 1165, 1640
Tru1I TTAA 4 cut(s) 402, 1220, 1487, 1520
Tru9I TTAA 4 cut(s) 402, 1220, 1487, 1520
TscAI CASTG 5 cut(s) 396, 598, 1036, 1609, 1622
TseFI GTSAC 3 cut(s) 406, 742, 1535
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 3 cut(s) 406, 742, 1535
TspRI CASTG 5 cut(s) 396, 598, 1036, 1609, 1622
VpaK11BI GGWCC 4 cut(s) 325, 356, 500, 710
XapI RAATTY 8 cut(s) 108, 415, 559, 767, 911, 934, 1191, 1549
XceI RCATGY 2 cut(s) 493, 1008
XcmI CCANNNNNNNNNTGG 2 cut(s) 510, 1490
XmnI GAANNNNTTC 2 cut(s) 274, 1393
XspI CTAG 4 cut(s) 267, 812, 1047, 1644
ZrmI AGTACT 2 cut(s) 664, 1543
Zsp2I ATGCAT 2 cut(s) 30, 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.