Rorug03G0281100
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001328
Physical Location & Seq
Reverse (-)
27933460 .. 27934082
623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0281100.1

Sequence Viewer

Length: 312 bp
ATGATTACACTCTTTGAGCTCACTTTTGCAAAACTTATTGAGTGTGTAAGATGCATAAAGAGGGATGCTTGTGGCTCATCTGTCATAAAAACTGACAAAACAACTCTGTCTGCAGATCATAATACGTACTTAATTGGAGATGATGAGCACTGCTACAGTGACAATCATGTGTCAAACATTGAAGGTGGTTCCTATGCTAAGTGCATTGATTTGTCAAAAGACAAGCAACCAGATATTTGGAATTCTATCAAATTTGAGACTGGCCAAATAAAGGAGGTCCTTCTAAGCTACACTCAAAGTTCATTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

103

Amino Acids

11.6

Weight (kDa)

5.06

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEPCK_ATP PF01293 31 - 87 4e-22 Phosphoenolpyruvate carboxykinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 241, 251
AfaI GTAC 1 cut(s) 128
AfiI CCNNNNNNNGG 1 cut(s) 271
AgsI TTSAA 1 cut(s) 182
AluBI AGCT 2 cut(s) 19, 288
AluI AGCT 2 cut(s) 19, 288
Alw21I GWGCWC 2 cut(s) 21, 150
Alw26I GTCTC 1 cut(s) 251
AoxI GGCC 1 cut(s) 262
ApoI RAATTY 2 cut(s) 241, 251
AspS9I GGNCC 1 cut(s) 277
AvaII GGWCC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 21, 150
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 2 cut(s) 111, 154
Bme18I GGWCC 1 cut(s) 277
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 190
BmsI GCATC 2 cut(s) 41, 55
BsaAI YACGTR 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 271
Bse1I ACTGG 1 cut(s) 265
BseGI GGATG 1 cut(s) 70
BseLI CCNNNNNNNGG 1 cut(s) 271
BseNI ACTGG 1 cut(s) 265
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 21, 150
BslI CCNNNNNNNGG 1 cut(s) 271
BsmAI GTCTC 1 cut(s) 251
BsnI GGCC 1 cut(s) 264
Bsp1286I GDGCHC 2 cut(s) 21, 150
Bsp143I GATC 1 cut(s) 115
BspANI GGCC 1 cut(s) 264
BspLI GGNNCC 1 cut(s) 190
BspMAI CTGCAG 1 cut(s) 115
BsrI ACTGG 1 cut(s) 265
BssMI GATC 1 cut(s) 115
Bst4CI ACNGT 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 198, 284
BstF5I GGATG 1 cut(s) 70
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 1 cut(s) 115
BstSFI CTRYAG 2 cut(s) 111, 154
BstSNI TACGTA 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 237
BsuRI GGCC 1 cut(s) 264
BtsCI GGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 148
BtsIMutI CAGTG 2 cut(s) 148, 163
Cfr13I GGNCC 1 cut(s) 277
Csp6I GTAC 1 cut(s) 127
CviAII CATG 1 cut(s) 167
CviJI RGCY 4 cut(s) 19, 75, 264, 288
CviKI_1 RGCY 4 cut(s) 19, 75, 264, 288
CviQI GTAC 1 cut(s) 127
DdeI CTNAG 2 cut(s) 198, 284
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 262
Ecl136II GAGCTC 1 cut(s) 19
Eco105I TACGTA 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 19
EcoO109I RGGNCCY 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 241
EcoT22I ATGCAT 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 21
FaeI CATG 1 cut(s) 170
FaiI YATR 5 cut(s) 56, 86, 120, 168, 195
FatI CATG 1 cut(s) 166
FokI GGATG 1 cut(s) 77
FriOI GRGCYC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 264
Hin1II CATG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 176, 290
HpyCH4III ACNGT 1 cut(s) 158
HpyCH4IV ACGT 1 cut(s) 125
HpyCH4V TGCA 4 cut(s) 29, 54, 113, 204
HpyF3I CTNAG 2 cut(s) 198, 284
HpySE526I ACGT 1 cut(s) 125
Hsp92II CATG 1 cut(s) 170
Kzo9I GATC 1 cut(s) 115
LpnPI CCDG 2 cut(s) 243, 246
LweI GCATC 2 cut(s) 41, 55
MaeII ACGT 1 cut(s) 125
MaeIII GTNAC 1 cut(s) 158
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MhlI GDGCHC 2 cut(s) 21, 150
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 3 cut(s) 132, 241, 251
MluNI TGGCCA 1 cut(s) 264
MnlI CCTC 2 cut(s) 54, 268
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 1 cut(s) 56
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 131, 310
Msp20I TGGCCA 1 cut(s) 264
NdeII GATC 1 cut(s) 115
NlaIII CATG 1 cut(s) 170
NlaIV GGNNCC 1 cut(s) 190
NmuCI GTSAC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 56
Ppu21I YACGTR 1 cut(s) 126
PpuMI RGGWCCY 1 cut(s) 277
Psp124BI GAGCTC 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 277
PspN4I GGNNCC 1 cut(s) 190
PspPI GGNCC 1 cut(s) 277
PspPPI RGGWCCY 1 cut(s) 277
PstI CTGCAG 1 cut(s) 115
RsaI GTAC 1 cut(s) 128
RsaNI GTAC 1 cut(s) 127
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 2 cut(s) 131, 310
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 277
SduI GDGCHC 2 cut(s) 21, 150
SetI ASST 5 cut(s) 21, 128, 187, 279, 290
SfaNI GCATC 2 cut(s) 41, 55
SfcI CTRYAG 2 cut(s) 111, 154
SgeI CNNG 5 cut(s) 81, 179, 235, 242, 273
SinI GGWCC 1 cut(s) 277
SnaBI TACGTA 1 cut(s) 126
Sse9I AATT 3 cut(s) 132, 241, 251
SstI GAGCTC 1 cut(s) 21
TaaI ACNGT 1 cut(s) 158
TaiI ACGT 1 cut(s) 128
TasI AATT 3 cut(s) 132, 241, 251
Tru1I TTAA 2 cut(s) 131, 310
Tru9I TTAA 2 cut(s) 131, 310
TscAI CASTG 2 cut(s) 155, 163
TseFI GTSAC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 1 cut(s) 291
TspRI CASTG 2 cut(s) 155, 163
VpaK11BI GGWCC 1 cut(s) 277
XapI RAATTY 2 cut(s) 241, 251
Zsp2I ATGCAT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.