pycom07g04400
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001328
Physical Location & Seq
Reverse (-)
3790292 .. 3794020
3729 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04400.1

Sequence Viewer

Length: 2370 bp
ATGGCGAACTCACATCTTCTTCTTCTTCTTCTTCTGTTGACATTTTCCCTCCTCTCCGTCCACCACTCAGCTGCTACTCAGTCAGAGTCCGAGAGCCTTCTGAAAATCCAGCAGCTGCTCAACCACCCACCACCTCTAGCCCGATTCAATTCCACTGACACACACTCTCTGTGCAGCATTGAACCAACTCCTTCCCTCACTCTCTATTGCTACCAAGGCAACGTCACCCAGCTCCACATTTCTGGCGGCGGAGCTGGAGCCGCCTTCCCTCCGCTGCCGCTGGACTTCTCAACCAACTCTCTGTTCTCCGCTCTCGCCGCCGCCTTCCCAACCCTCAAAGTCCTCTCTCTCGTTTCACTCCAACTCTGGGGGCCACTCCCTCCCTCCATCGCCCGTTTATCTTCCCTCGAGATTCTCAACCTCAACTCCAACTACCTCAGCGGCGCCATTCCTCCCCACATTTCGTCGCTCGTCAACCTCCAGACTCTCATCCTCGATCGCAACAACTTCACCGGCCGACTCCCGGCGTCGTTGGGTTCGCTTCCGTCGTTGGCCGTTCTCAGTTTGAACCACAACCTCCTCAACGGCCCTCTCCCGAGTTCTCTGGCCTCTCTGCAAACCCTCAGAGTCCTCTCTCTCTCCCACAACTTCATGTCCGGCCAAGTTCCCGACCTCACAGCCCTCACAAACATGCAACTTCTTGATTTGGAAGCCAACTCTTTTGGGCCTCATTTTCCAAGCTTGCCTTACAACAACAAGTTGGTAACCCTCGTGCTCCGAAACAACACGTTTCGCGTCGGAATCCAAACCGGATTCCAAGGCGGTTTTCAGCTCCAGAAGCTTGACATTTCACTCAATGAATTCGTGGGGCCTTTTTCGCCGTCGTGGCTGTCGCTGCCATCCATCAATTATCTTGACATCGCCGGAAATAAATTCACCGGCGTGCTCTCCAAGAACATATCTTGCAATGCTGAGCTTGCTTTTGTGAATTTGTCATCAAATCTTTTGGCAGGGGACTTGCCCACTTGCCTCAAAAACCGGGGTTCGAAGAACAACAGGGCTGTTGTTCTGTATTCCGGGAATTGTTTGACGAATGTAGATCAGCAGCAGCCTTCTAACTTGTGCCACAATGAAGCTCTGGCTGTGGAGTTACCGCCTTCCGGTGACCGAAAAATCAAGAGGCGTCATGATAATAAACAAATCGTTGCATCAAGTGCCGTGGGAGGAATTGTGGGTGCTGCTGCAGTTGTTCTTCTGGCTTTCATGGCTGTTAAGAGGTTATTGCACGGCGAACAGCCTGTTAAAAGTCCCCGAACAAGGTTGATTAGAGATTCAGCTTCAACAGTCAACACAGCCAAGCTACTCTCGGATGCAAAATATATATCGGAAACAATGAAGCTGGGAGCTAGCCTTCCTGCTTATCGAACGTTTGCTTTGGAGGAGCTCAAGGAAGCTACCAACGACTTTGATGATTCAACATTACTTGGCGAAGGTGCCCATGGACAGATGTATAGAGGGAAGCTCCCCGACGGAACACTTGTTGCTATCAAGGGCTTGAAGATGAGAAAGAGGCAGGCTACACACGTCTATACACACCTCCTTGAGCTGGTTTCGAAACTGAGACATAGCCATTTGGTTAGCGCTCTTGGACATTGCTTGGAGTACAATCCTGATGATTCGGGCGTCAGTAGAATATTTCTTGTATTCGAGTTTGTTCCAAATGGAACGCTAAGAGGCTTCATCTCTGAAGGACCTCCAGAACGAAAATTTACTTGGGCGCAGAGAATAGTAGCTGCAATTGGAGTTGCAAAGGGTGTTCAGTTTCTGCATACAGGGATTGTTCCTGGTGTAAAATCAAATAATCTCAAGATAACAAATGTCTTGTTGGATCACGATCTTCATGTGAAAATAAGCAGTTATAACCTACCTCTTTTAGCTGAAAACAACAGGGGAACGGTGGGGACTGCAGTTTCTTCTCCAGCACCAAAAGGAAGCATTCATACTAGGGTTAACCATGAGTGTAAGAACGACGTTTATGACATTGGAGTAATCTTGCTAGAAATGATTCTGGGGAGACCTATCATGTTCCCAAATGAAGTTGGTGTTTTGAAAGACCTTGTACGAGTAAGTTTGACGATTGATGACACAGCTCGGAGGAGCATTGTGGATGCAGCAGTGCACAAGGAATGCTCGGACAAATCGCTGAAGACAATGATGGAGATATGCGTGAGGTGTCTTACGAATGATGCAGCTGATAGGCCTTCAGTTGAAGACATTCTTTGGAATTTGCAGTTTGCAGCGCAGGTTCAGGAATCAGCAAGAGAAGACTGTCGGAGTAGTAGTCAAGGTTCACCCGTTTCATCACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

790

Amino Acids

85.46

Weight (kDa)

8.69

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 107 - 308 6e-12 Leucine-rich repeat region
LRR_8 PF13855 159 - 216 2.3e-06 Leucine rich repeat
Pkinase PF00069 491 - 760 5e-19 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 494 - 762 1.2e-20 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1920
Acc36I ACCTGC 1 cut(s) 2293
AccB1I GGYRCC 2 cut(s) 443, 1493
AccBSI CCGCTC 1 cut(s) 311
AccII CGCG 1 cut(s) 795
AclI AACGTT 1 cut(s) 1427
AclWI GGATC 1 cut(s) 1896
AcoI YGGCCR 3 cut(s) 514, 552, 658
AcsI RAATTY 5 cut(s) 860, 932, 988, 1766, 2284
AcuI CTGAAG 3 cut(s) 1767, 2225, 2247
AcyI GRCGYC 4 cut(s) 444, 527, 1183, 1683
AfaI GTAC 2 cut(s) 1664, 2121
AfeI AGCGCT 1 cut(s) 1642
AfiI CCNNNNNNNGG 5 cut(s) 367, 523, 1160, 1317, 1606
AflIII ACRYGT 2 cut(s) 786, 1582
AgsI TTSAA 8 cut(s) 148, 182, 568, 1341, 1476, 1558, 2110, 2270
AjiI CACGTC 1 cut(s) 1585
AjnI CCWGG 1 cut(s) 1843
AjuI GAANNNNNNNTTGG 6 cut(s) 287, 319, 810, 842, 1756, 1788
AleI CACNNNNGTG 1 cut(s) 941
Alw21I GWGCWC 4 cut(s) 777, 948, 1446, 2181
Alw26I GTCTC 2 cut(s) 1615, 2068
Alw44I GTGCAC 1 cut(s) 2177
AlwI GGATC 1 cut(s) 1896
AlwNI CAGNNNCTG 2 cut(s) 115, 1825
Ama87I CYCGRG 2 cut(s) 407, 595
Aor51HI AGCGCT 1 cut(s) 1642
AoxI GGCC 9 cut(s) 371, 514, 552, 586, 606, 658, 725, 869, 2258
ApaLI GTGCAC 1 cut(s) 2177
ApoI RAATTY 5 cut(s) 860, 932, 988, 1766, 2284
Asp700I GAANNNNTTC 2 cut(s) 2064, 2274
AspLEI GCGC 4 cut(s) 446, 1643, 1780, 2302
AspS9I GGNCC 5 cut(s) 371, 587, 725, 869, 1751
AsuC2I CCSGG 3 cut(s) 524, 1040, 1078
AsuHPI GGTGA 6 cut(s) 217, 502, 928, 1175, 2343, 2355
AsuII TTCGAA 2 cut(s) 1046, 1613
AsuNHI GCTAGC 1 cut(s) 1406
AvaI CYCGRG 2 cut(s) 407, 595
AvaII GGWCC 1 cut(s) 1751
BaeGI GKGCMC 2 cut(s) 1498, 2181
BanI GGYRCC 2 cut(s) 443, 1493
BanII GRGCYC 1 cut(s) 1446
BarI GAAGNNNNNNTAC 2 cut(s) 1984, 2016
BauI CACGAG 1 cut(s) 770
BbsI GAAGAC 3 cut(s) 2213, 2277, 2331
Bbv12I GWGCWC 4 cut(s) 777, 948, 1446, 2181
BbvCI CCTCAGC 1 cut(s) 437
BccI CCATC 4 cut(s) 395, 907, 911, 2209
BceAI ACGGC 5 cut(s) 539, 601, 865, 1202, 1303
BciT130I CCWGG 1 cut(s) 1845
BcnI CCSGG 3 cut(s) 524, 1040, 1078
BcoDI GTCTC 2 cut(s) 1615, 2068
BfaI CTAG 4 cut(s) 137, 1407, 2004, 2057
BfmI CTRYAG 2 cut(s) 1242, 1965
BfoI RGCGCY 2 cut(s) 447, 1644
BfuAI ACCTGC 1 cut(s) 2293
BglI GCCNNNNNGGC 1 cut(s) 886
BlpI GCTNAGC 1 cut(s) 972
Bme1390I CCNGG 4 cut(s) 524, 1040, 1078, 1845
Bme18I GGWCC 1 cut(s) 1751
BmeT110I CYCGRG 2 cut(s) 407, 595
BmgBI CACGTC 1 cut(s) 1585
BmgT120I GGNCC 5 cut(s) 371, 587, 725, 869, 1751
BmiI GGNNCC 5 cut(s) 259, 372, 445, 870, 1495
BmrFI CCNGG 4 cut(s) 524, 1040, 1078, 1845
BmsI GCATC 4 cut(s) 1217, 1360, 2158, 2236
BmtI GCTAGC 1 cut(s) 1410
BpiI GAAGAC 3 cut(s) 2213, 2277, 2331
BplI GAGNNNNNCTC 2 cut(s) 1506, 1538
BpmI CTGGAG 5 cut(s) 276, 464, 818, 1740, 1962
Bpu10I CCTNAGC 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 972
Bpu14I TTCGAA 2 cut(s) 1046, 1613
BpuEI CTTGAG 3 cut(s) 1430, 1622, 1850
BpuMI CCSGG 3 cut(s) 524, 1040, 1078
BsaBI GATNNNNATC 1 cut(s) 1893
BsaHI GRCGYC 4 cut(s) 444, 527, 1183, 1683
BsaI GGTCTC 1 cut(s) 2068
BsaJI CCNNGG 5 cut(s) 214, 817, 1039, 1218, 1498
BsaWI WCCGGW 2 cut(s) 809, 1160
BsaXI ACNNNNNCTCC 6 cut(s) 410, 440, 1215, 1245, 2326, 2356
Bsc4I CCNNNNNNNGG 5 cut(s) 367, 523, 1160, 1317, 1606
Bse118I RCCGGY 2 cut(s) 512, 938
Bse3DI GCAATG 2 cut(s) 973, 1651
Bse8I GATNNNNATC 1 cut(s) 1893
BseBI CCWGG 1 cut(s) 1845
BseDI CCNNGG 5 cut(s) 214, 817, 1039, 1218, 1498
BseGI GGATG 4 cut(s) 489, 899, 1375, 2173
BseJI GATNNNNATC 1 cut(s) 1893
BseLI CCNNNNNNNGG 5 cut(s) 367, 523, 1160, 1317, 1606
BseMI GCAATG 2 cut(s) 973, 1651
BseMII CTCAG 7 cut(s) 81, 92, 451, 574, 637, 963, 1610
BseRI GAGGAG 4 cut(s) 41, 569, 1454, 2170
BseSI GKGCMC 2 cut(s) 1498, 2181
BseX3I CGGCCG 1 cut(s) 514
BseYI CCCAGC 2 cut(s) 228, 1399
BsgI GTGCAG 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 795
Bsh1285I CGRYCG 2 cut(s) 499, 517
BshFI GGCC 9 cut(s) 373, 516, 554, 588, 608, 660, 727, 871, 2260
BshNI GGYRCC 2 cut(s) 443, 1493
BsiEI CGRYCG 2 cut(s) 499, 517
BsiHKAI GWGCWC 4 cut(s) 777, 948, 1446, 2181
BsiHKCI CYCGRG 2 cut(s) 407, 595
BsiSI CCGG 9 cut(s) 513, 524, 657, 810, 924, 939, 1039, 1077, 1161
BslFI GGGAC 3 cut(s) 1028, 1293, 1975
BslI CCNNNNNNNGG 5 cut(s) 367, 523, 1160, 1317, 1606
BsmAI GTCTC 2 cut(s) 1615, 2068
BsmFI GGGAC 3 cut(s) 1028, 1293, 1975
BsmI GAATGC 2 cut(s) 1995, 2192
BsnI GGCC 9 cut(s) 373, 516, 554, 588, 608, 660, 727, 871, 2260
Bso31I GGTCTC 1 cut(s) 2068
BsoBI CYCGRG 2 cut(s) 407, 595
Bsp119I TTCGAA 2 cut(s) 1046, 1613
Bsp1286I GDGCHC 5 cut(s) 777, 948, 1446, 1498, 2181
Bsp143I GATC 4 cut(s) 496, 1099, 1888, 1894
Bsp1720I GCTNAGC 1 cut(s) 972
Bsp19I CCATGG 1 cut(s) 1498
BspANI GGCC 9 cut(s) 373, 516, 554, 588, 608, 660, 727, 871, 2260
BspCNI CTCAG 7 cut(s) 80, 91, 450, 573, 636, 964, 1611
BspFNI CGCG 1 cut(s) 795
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 5 cut(s) 259, 372, 445, 870, 1495
BspMAI CTGCAG 2 cut(s) 1246, 1969
BspMI ACCTGC 1 cut(s) 2293
BspOI GCTAGC 1 cut(s) 1410
BspPI GGATC 1 cut(s) 1896
BspT104I TTCGAA 2 cut(s) 1046, 1613
BspT107I GGYRCC 2 cut(s) 443, 1493
BspTNI GGTCTC 1 cut(s) 2068
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 973, 1651
BsrFI RCCGGY 2 cut(s) 512, 938
BssAI RCCGGY 2 cut(s) 512, 938
BssECI CCNNGG 5 cut(s) 214, 817, 1039, 1218, 1498
BssMI GATC 4 cut(s) 496, 1099, 1888, 1894
BssNI GRCGYC 4 cut(s) 444, 527, 1183, 1683
BssSI CACGAG 1 cut(s) 770
BssT1I CCWWGG 3 cut(s) 214, 817, 1498
Bst2BI CACGAG 1 cut(s) 770
Bst2UI CCWGG 1 cut(s) 1845
Bst4CI ACNGT 3 cut(s) 1345, 1957, 2330
BstACI GRCGYC 4 cut(s) 444, 527, 1183, 1683
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstBI TTCGAA 2 cut(s) 1046, 1613
BstC8I GCNNGC 5 cut(s) 743, 944, 978, 1408, 1575
BstDEI CTNAG 8 cut(s) 67, 78, 437, 560, 623, 972, 1619, 1730
BstDSI CCRYGG 2 cut(s) 1218, 1498
BstEII GGTNACC 2 cut(s) 763, 1163
BstF5I GGATG 4 cut(s) 489, 899, 1375, 2173
BstFNI CGCG 1 cut(s) 795
BstH2I RGCGCY 2 cut(s) 447, 1644
BstHHI GCGC 4 cut(s) 446, 1643, 1780, 2302
BstKTI GATC 4 cut(s) 499, 1102, 1891, 1897
BstMAI GTCTC 2 cut(s) 1615, 2068
BstMBI GATC 4 cut(s) 496, 1099, 1888, 1894
BstMCI CGRYCG 2 cut(s) 499, 517
BstNI CCWGG 1 cut(s) 1845
BstNSI RCATGY 1 cut(s) 694
BstPI GGTNACC 2 cut(s) 763, 1163
BstSCI CCNGG 4 cut(s) 522, 1038, 1076, 1843
BstSFI CTRYAG 2 cut(s) 1242, 1965
BstSLI GKGCMC 2 cut(s) 1498, 2181
BstUI CGCG 1 cut(s) 795
BstV2I GAAGAC 3 cut(s) 2213, 2277, 2331
BstXI CCANNNNNNTGG 1 cut(s) 242
BstZI CGGCCG 1 cut(s) 514
BsuRI GGCC 9 cut(s) 373, 516, 554, 588, 608, 660, 727, 871, 2260
BtgI CCRYGG 2 cut(s) 1218, 1498
BtgZI GCGATG 2 cut(s) 373, 904
BtrI CACGTC 1 cut(s) 1585
BtsCI GGATG 4 cut(s) 489, 899, 1375, 2173
BtsI GCAGTG 1 cut(s) 2181
BtsIMutI CAGTG 2 cut(s) 153, 2181
BveI ACCTGC 1 cut(s) 2293
Cac8I GCNNGC 5 cut(s) 743, 944, 978, 1408, 1575
CaiI CAGNNNCTG 2 cut(s) 115, 1825
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 4 cut(s) 446, 1643, 1780, 2302
Cfr10I RCCGGY 2 cut(s) 512, 938
Cfr13I GGNCC 5 cut(s) 371, 587, 725, 869, 1751
CseI GACGC 4 cut(s) 516, 784, 1172, 1672
Csp6I GTAC 2 cut(s) 1663, 2120
CspCI CAANNNNNGTGG 2 cut(s) 1200, 1235
CviAII CATG 8 cut(s) 652, 691, 1187, 1264, 1499, 1901, 2015, 2083
CviQI GTAC 2 cut(s) 1663, 2120
DdeI CTNAG 8 cut(s) 67, 78, 437, 560, 623, 972, 1619, 1730
DinI GGCGCC 1 cut(s) 445
DpnI GATC 4 cut(s) 498, 1101, 1890, 1896
DpnII GATC 4 cut(s) 496, 1099, 1888, 1894
EaeI YGGCCR 3 cut(s) 514, 552, 658
EagI CGGCCG 1 cut(s) 514
EciI GGCGGA 1 cut(s) 264
Ecl136II GAGCTC 1 cut(s) 1444
EclXI CGGCCG 1 cut(s) 514
Eco130I CCWWGG 3 cut(s) 214, 817, 1498
Eco147I AGGCCT 1 cut(s) 2260
Eco24I GRGCYC 1 cut(s) 1446
Eco31I GGTCTC 1 cut(s) 2068
Eco47I GGWCC 1 cut(s) 1751
Eco47III AGCGCT 1 cut(s) 1642
Eco52I CGGCCG 1 cut(s) 514
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 3 cut(s) 1767, 2225, 2247
Eco88I CYCGRG 2 cut(s) 407, 595
Eco91I GGTNACC 2 cut(s) 763, 1163
EcoICRI GAGCTC 1 cut(s) 1444
EcoO109I RGGNCCY 2 cut(s) 869, 1751
EcoO65I GGTNACC 2 cut(s) 763, 1163
EcoRI GAATTC 1 cut(s) 860
EcoRII CCWGG 1 cut(s) 1843
EcoT14I CCWWGG 3 cut(s) 214, 817, 1498
EcoT38I GRGCYC 1 cut(s) 1446
EgeI GGCGCC 1 cut(s) 445
EheI GGCGCC 1 cut(s) 445
ErhI CCWWGG 3 cut(s) 214, 817, 1498
FaeI CATG 8 cut(s) 655, 694, 1190, 1267, 1502, 1904, 2018, 2086
FalI AAGNNNNNCTT 4 cut(s) 730, 762, 2262, 2294
FaqI GGGAC 3 cut(s) 1028, 1293, 1975
FatI CATG 8 cut(s) 651, 690, 1186, 1263, 1498, 1900, 2014, 2082
FokI GGATG 4 cut(s) 476, 886, 1382, 2180
FriOI GRGCYC 1 cut(s) 1446
FspBI CTAG 4 cut(s) 137, 1407, 2004, 2057
GlaI GCGC 4 cut(s) 445, 1642, 1779, 2301
GsaI CCCAGC 2 cut(s) 232, 1403
GsuI CTGGAG 5 cut(s) 276, 464, 818, 1740, 1962
HaeII RGCGCY 2 cut(s) 447, 1644
HaeIII GGCC 9 cut(s) 373, 516, 554, 588, 608, 660, 727, 871, 2260
HapII CCGG 9 cut(s) 513, 524, 657, 810, 924, 939, 1039, 1077, 1161
HgaI GACGC 4 cut(s) 516, 784, 1172, 1672
HhaI GCGC 4 cut(s) 446, 1643, 1780, 2302
Hin1I GRCGYC 4 cut(s) 444, 527, 1183, 1683
Hin1II CATG 8 cut(s) 655, 694, 1190, 1267, 1502, 1904, 2018, 2086
Hin6I GCGC 4 cut(s) 444, 1641, 1778, 2300
HinP1I GCGC 4 cut(s) 444, 1641, 1778, 2300
HincII GTYRAC 4 cut(s) 39, 475, 1348, 2011
HindII GTYRAC 4 cut(s) 39, 475, 1348, 2011
HindIII AAGCTT 2 cut(s) 739, 839
HpaI GTTAAC 1 cut(s) 2011
HpaII CCGG 9 cut(s) 513, 524, 657, 810, 924, 939, 1039, 1077, 1161
HphI GGTGA 6 cut(s) 217, 502, 928, 1175, 2343, 2355
Hpy166II GTNNAC 7 cut(s) 39, 61, 475, 1348, 2011, 2179, 2351
Hpy8I GTNNAC 7 cut(s) 39, 61, 475, 1348, 2011, 2179, 2351
Hpy99I CGWCG 7 cut(s) 469, 532, 550, 800, 886, 1532, 2033
HpyCH4III ACNGT 3 cut(s) 1345, 1957, 2330
HpyCH4IV ACGT 5 cut(s) 222, 788, 1427, 1584, 2031
HpyF3I CTNAG 8 cut(s) 67, 78, 437, 560, 623, 972, 1619, 1730
HpySE526I ACGT 5 cut(s) 222, 788, 1427, 1584, 2031
Hsp92I GRCGYC 4 cut(s) 444, 527, 1183, 1683
Hsp92II CATG 8 cut(s) 655, 694, 1190, 1267, 1502, 1904, 2018, 2086
HspAI GCGC 4 cut(s) 444, 1641, 1778, 2300
KasI GGCGCC 1 cut(s) 443
KspAI GTTAAC 1 cut(s) 2011
Kzo9I GATC 4 cut(s) 496, 1099, 1888, 1894
LmnI GCTCC 9 cut(s) 237, 251, 257, 780, 837, 1403, 1441, 1527, 2157
LweI GCATC 4 cut(s) 1217, 1360, 2158, 2236
MaeI CTAG 4 cut(s) 137, 1407, 2004, 2057
MaeII ACGT 5 cut(s) 222, 788, 1427, 1584, 2031
MaeIII GTNAC 4 cut(s) 223, 763, 1149, 1163
MalI GATC 4 cut(s) 498, 1101, 1890, 1896
MbiI CCGCTC 1 cut(s) 311
MboI GATC 4 cut(s) 496, 1099, 1888, 1894
MfeI CAATTG 1 cut(s) 1797
MhlI GDGCHC 5 cut(s) 777, 948, 1446, 1498, 2181
Mly113I GGCGCC 1 cut(s) 444
MlyI GAGTC 4 cut(s) 95, 478, 513, 636
MmeI TCCRAC 5 cut(s) 385, 453, 778, 1866, 2312
MroXI GAANNNNTTC 2 cut(s) 2064, 2274
MseI TTAA 3 cut(s) 1272, 1302, 2010
MslI CAYNNNNRTG 2 cut(s) 689, 941
MspA1I CMGCKG 6 cut(s) 71, 115, 274, 280, 441, 2252
MspI CCGG 9 cut(s) 513, 524, 657, 810, 924, 939, 1039, 1077, 1161
MspR9I CCNGG 4 cut(s) 524, 1040, 1078, 1845
MunI CAATTG 1 cut(s) 1797
Mva1269I GAATGC 2 cut(s) 1995, 2192
MvaI CCWGG 1 cut(s) 1845
MvnI CGCG 1 cut(s) 795
NarI GGCGCC 1 cut(s) 444
NciI CCSGG 3 cut(s) 524, 1040, 1078
NcoI CCATGG 1 cut(s) 1498
NdeII GATC 4 cut(s) 496, 1099, 1888, 1894
NheI GCTAGC 1 cut(s) 1406
NlaIII CATG 8 cut(s) 655, 694, 1190, 1267, 1502, 1904, 2018, 2086
NlaIV GGNNCC 5 cut(s) 259, 372, 445, 870, 1495
NmuCI GTSAC 2 cut(s) 223, 1163
NspI RCATGY 1 cut(s) 694
NspV TTCGAA 2 cut(s) 1046, 1613
OliI CACNNNNGTG 1 cut(s) 941
PaeR7I CTCGAG 1 cut(s) 407
PagI TCATGA 1 cut(s) 1186
PceI AGGCCT 1 cut(s) 2260
PcsI WCGNNNNNNNCGW 1 cut(s) 545
PctI GAATGC 2 cut(s) 1995, 2192
PdmI GAANNNNTTC 2 cut(s) 2064, 2274
PfeI GAWTC 9 cut(s) 144, 412, 801, 813, 1331, 1472, 1676, 2065, 2312
PfoI TCCNGGA 1 cut(s) 1076
Ple19I CGATCG 1 cut(s) 499
PleI GAGTC 4 cut(s) 94, 478, 513, 635
PluTI GGCGCC 1 cut(s) 447
PpsI GAGTC 4 cut(s) 94, 478, 513, 635
PpuMI RGGWCCY 1 cut(s) 1751
PsiI TTATAA 1 cut(s) 1920
Psp124BI GAGCTC 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 1427
Psp5II RGGWCCY 1 cut(s) 1751
Psp6I CCWGG 1 cut(s) 1843
PspEI GGTNACC 2 cut(s) 763, 1163
PspFI CCCAGC 2 cut(s) 228, 1399
PspGI CCWGG 1 cut(s) 1843
PspN4I GGNNCC 5 cut(s) 259, 372, 445, 870, 1495
PspPI GGNCC 5 cut(s) 371, 587, 725, 869, 1751
PspPPI RGGWCCY 1 cut(s) 1751
PsrI GAACNNNNNNTAC 2 cut(s) 2332, 2364
PstI CTGCAG 2 cut(s) 1246, 1969
PstNI CAGNNNCTG 2 cut(s) 115, 1825
PvuI CGATCG 1 cut(s) 499
PvuII CAGCTG 3 cut(s) 71, 115, 2252
RsaI GTAC 2 cut(s) 1664, 2121
RsaNI GTAC 2 cut(s) 1663, 2120
RseI CAYNNNNRTG 2 cut(s) 689, 941
SacI GAGCTC 1 cut(s) 1446
SaqAI TTAA 3 cut(s) 1272, 1302, 2010
Sau3AI GATC 4 cut(s) 496, 1099, 1888, 1894
Sau96I GGNCC 5 cut(s) 371, 587, 725, 869, 1751
SchI GAGTC 4 cut(s) 95, 478, 513, 636
ScrFI CCNGG 4 cut(s) 524, 1040, 1078, 1845
SduI GDGCHC 5 cut(s) 777, 948, 1446, 1498, 2181
SfaNI GCATC 4 cut(s) 1217, 1360, 2158, 2236
SfcI CTRYAG 2 cut(s) 1242, 1965
SfoI GGCGCC 1 cut(s) 445
Sfr274I CTCGAG 1 cut(s) 407
SfuI TTCGAA 2 cut(s) 1046, 1613
SgrAI CRCCGGYG 1 cut(s) 938
SinI GGWCC 1 cut(s) 1751
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 2 cut(s) 689, 941
SmlI CTYRAG 4 cut(s) 407, 1445, 1601, 1865
SmoI CTYRAG 4 cut(s) 407, 1445, 1601, 1865
SseBI AGGCCT 1 cut(s) 2260
SspDI GGCGCC 1 cut(s) 443
SspI AATATT 1 cut(s) 1695
SspMI CTAG 4 cut(s) 137, 1407, 2004, 2057
SstI GAGCTC 1 cut(s) 1446
StuI AGGCCT 1 cut(s) 2260
StyD4I CCNGG 4 cut(s) 522, 1038, 1076, 1843
StyI CCWWGG 3 cut(s) 214, 817, 1498
TaaI ACNGT 3 cut(s) 1345, 1957, 2330
TaiI ACGT 5 cut(s) 225, 791, 1430, 1587, 2034
TaqI TCGA 6 cut(s) 408, 495, 1046, 1423, 1613, 1707
TaqII GACCGA 1 cut(s) 1182
TatI WGTACW 1 cut(s) 1662
TauI GCSGC 6 cut(s) 249, 263, 280, 320, 323, 444
TfiI GAWTC 9 cut(s) 144, 412, 801, 813, 1331, 1472, 1676, 2065, 2312
Tru1I TTAA 3 cut(s) 1272, 1302, 2010
Tru9I TTAA 3 cut(s) 1272, 1302, 2010
TscAI CASTG 2 cut(s) 160, 2181
TseFI GTSAC 2 cut(s) 223, 1163
Tsp45I GTSAC 2 cut(s) 223, 1163
TspGWI ACGGA 3 cut(s) 46, 534, 1545
TspRI CASTG 2 cut(s) 160, 2181
VneI GTGCAC 1 cut(s) 2177
VpaK11BI GGWCC 1 cut(s) 1751
XapI RAATTY 5 cut(s) 860, 932, 988, 1766, 2284
XceI RCATGY 1 cut(s) 694
XhoI CTCGAG 1 cut(s) 407
XmnI GAANNNNTTC 2 cut(s) 2064, 2274
XspI CTAG 4 cut(s) 137, 1407, 2004, 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.