MD07G1057800.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001328
Physical Location & Seq
Reverse (-)
5352067 .. 5356271
4205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1057800.v1.1.491

Sequence Viewer

Length: 2370 bp
ATGGCGAACTCACATCTTCTTCTTCTTCTTCTGTTGACATTTTCCCTCCTCTCCGTCCACCACTCAGCTGCTACTCAGTCAGAGTCCGAGAGCCTTCTGAAAATCCAGCAGCTGCTCAACCACCCACCACCTCTAACCCGAATCAATTCCACTGACAGACACTCTCTGTGCAGCATTGAACCAACTCCTTCCCTCACTATTTCTTGCCACCAAGGCAACGTCACCCAGCTCCACATTTCTGGCGGCGGAGATGGAGCCGCCTTCCCTCCGCTGCCGCTGGACTTCTCAACCCACTCTCTGTTCTCCGCTCTCGCCGCCGCCTTCCCAACCCTCAAAGTCCTCTCTCTCGTTTCACTCCGACTCTGGGGGCCACTCCCTCCCTCCATCGCCCGTTTATCTTCCCTCGAGATTCTCAACCTCAACTCCAACTACCTCAGCGGCGCCATACCTTCCCATATTTCGTCTCTCGTCAACCTCCAGACTCTCATCCTCGACCGCAACAACTTCACAGGCCAACTCCCGCCGTCGCTGGGTTCGCTTCCGTCGTTGGCCGTTCTCAGTTTGAACCACAATCTCCTCAACGGCCCTCTCCCGAGTTCTCTGGCCTCTCTGCAATCCCTCAGAGTCCTCTCTCTCTCCCACAACTTCCTGTCCGGCGAAGTTCCCGACCTCACAGCCCTCACAAACATGCAACTTCTTGATTTGGAAGCCAACTATTTTGGGCCTCATTTTCCAAGCTTGCCTTCCAAGAACAAGCTGGTAACCCTCGTGCTCCGAAACAACACGTTTCGCGTCGGAATCCAAACCGGATTCCAAGGCGGTTTTCAGCTCCAGAAGCTTGACATTTCACTCAATGAATTCGTGGGGCCTTTTTCGCCGTCGTGGCTGTCGCTGCCATCCATCAATTATCTTGACGTCGCCGGAAATAAATTCACCGGCGTGCTCTCCAAGAACATGTCTTGCAATGTTGAGCTTGCTTTTGTGAATTTGTCATCAAATCTTTTGGCAGGGGACTTGCCCACTTGCCTCAAAAACCGGGGTTCGAAGAACAACAGGGCTGTTGTTCTGTATTCCGGGAATTGTTTGACGAATGTAGATCAGCAGCAGCCTTCTAACTTGTGCCACAATGAAGCTCTGGCTGTGGACTTACCACCTTCTGGTGACGGAAAAATCAAGAGGCGTCATGATAACAAACAAGTCGTTGCATCAAGTGCCGTGGGAGGAATTGTGGGTGCTGCTGCAGTTGTTGTTCTGGCTTTCATGGCTGTTAAGAGGTTATTGTACAGCAAACAGCCGGTCAAAAGTCCCCCAACAAGGTTGATTAGAGATTCAGCTTCACCAGTCAACACAGCCAAGCTACTCTCGGATGCAAAATATATATCAGAAACAATGAAGCTGGGAGCTAGCCTTCCTGCTTATCGAACGTTTGCTTTGGAGGAGCTCAAGGAAGCTACCAACGACTTCGATGATTCAACGTTACTTGGCGAAGGTGCACATGGACAGCGCCAGATGTATAGAGGGAAGCTCCCCGACGGAACACTTGTTGCTATCAGAGGCTTGAAAATTAGAAAGAGGCAGGCTACACAGGTCTATACACACCTCCTTGAGCTGGTTTCGAAACTGAGACATAGCCATTTGGTTAGCGCTCTTGGACATTGCTTGGAGTACAATCCTGATGATTCGGGCGTCAGTAGAATATTTCTTGTATTCGAGTTTTTTCCAAATGGAACGCTAAGAGGCTTCATCTCTGGACCTCCAGCACGAAAATTTACTTGGGCGCAGAGAATAGTAGCTGCAATCGGAGTTGCAAAGGGCGTTCAGTTTCTGCATACAGGGATTGTTCCTGGTGTAAAATCAAATAATCTCAAGATAACAAATGTCTTATTGGATCACGATCTTCACGTGAAAATAAGCAATTACAACCTACCTCTTTTAGCCGAAAACAACAGGGGAACGGTGGGGACTGCAGTTTCTTCTCCTGCACCAAAAGGAAGCATTCATACTAGGGTTAACCATGAGTGTAAGAACGACGTTTATGACATTGGAGTAATCTTGCTAGAAATGATTCTGGGGAGACCTATCATGTTCCGAAATGAAGTTGGTGTTTTGAAAGACCTTGTACGAGTAAGTTTGACGATTGATGACACAGCTCGGAGGAGCATTGTGGATGCAGCAGTGCACAAGGAATGCTCGGACGAATCGCTGAAGATAATGATGGAGATATGCGTGAGATGTCTTACGAATGATGCAGCTGATAGGCCTTCAGTTGAAGACATTCTTTGGAATCTGCAGTTTGCAGCGCAGGTTCAGGAATCAGCAAGAGAAGACTGTCCGAGTAGTAGTCAAGGCTCGCCCGTTTCATCACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

790

Amino Acids

85.43

Weight (kDa)

8.78

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 106 - 306 3.4e-12 Leucine-rich repeat region
LRR_8 PF13855 159 - 215 4.2e-06 Leucine rich repeat
Pkinase PF00069 491 - 760 1.5e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 761 2.8e-16 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 918
Acc36I ACCTGC 1 cut(s) 2293
AccB1I GGYRCC 1 cut(s) 440
AccB7I CCANNNNNTGG 1 cut(s) 1157
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 1 cut(s) 792
AclI AACGTT 2 cut(s) 1424, 1475
AclWI GGATC 1 cut(s) 1896
AcoI YGGCCR 1 cut(s) 549
AcsI RAATTY 4 cut(s) 857, 929, 985, 1766
AcuI CTGAAG 2 cut(s) 2225, 2247
AcvI CACGTG 1 cut(s) 1903
AcyI GRCGYC 4 cut(s) 441, 915, 1180, 1686
AfaI GTAC 3 cut(s) 1283, 1667, 2121
AfeI AGCGCT 1 cut(s) 1645
AfiI CCNNNNNNNGG 5 cut(s) 364, 530, 1157, 1314, 1609
AflIII ACRYGT 2 cut(s) 783, 954
AgsI TTSAA 6 cut(s) 179, 565, 1473, 1561, 2110, 2270
AjnI CCWGG 1 cut(s) 1843
AjuI GAANNNNNNNTTGG 6 cut(s) 727, 759, 807, 839, 1756, 1788
AleI CACNNNNGTG 1 cut(s) 938
Alw21I GWGCWC 5 cut(s) 774, 945, 1443, 1495, 2181
Alw26I GTCTC 3 cut(s) 468, 1618, 2068
Alw44I GTGCAC 2 cut(s) 1491, 2177
AlwI GGATC 1 cut(s) 1896
AlwNI CAGNNNCTG 2 cut(s) 112, 1825
Ama87I CYCGRG 2 cut(s) 404, 592
Aor51HI AGCGCT 1 cut(s) 1645
AoxI GGCC 8 cut(s) 368, 511, 549, 583, 603, 722, 866, 2258
ApaLI GTGCAC 2 cut(s) 1491, 2177
ApoI RAATTY 4 cut(s) 857, 929, 985, 1766
ArsI GACNNNNNNTTYG 2 cut(s) 1281, 1313
Asp700I GAANNNNTTC 3 cut(s) 145, 2064, 2274
AspLEI GCGC 5 cut(s) 443, 1506, 1646, 1780, 2302
AspS9I GGNCC 5 cut(s) 368, 584, 722, 866, 1751
AsuC2I CCSGG 2 cut(s) 1037, 1075
AsuHPI GGTGA 5 cut(s) 214, 925, 1172, 1329, 2355
AsuII TTCGAA 2 cut(s) 1043, 1616
AsuNHI GCTAGC 1 cut(s) 1403
AvaI CYCGRG 2 cut(s) 404, 592
AvaII GGWCC 1 cut(s) 1751
BaeGI GKGCMC 2 cut(s) 1495, 2181
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 1443
BarI GAAGNNNNNNTAC 2 cut(s) 1984, 2016
BauI CACGAG 1 cut(s) 767
BbrPI CACGTG 1 cut(s) 1903
BbsI GAAGAC 2 cut(s) 2277, 2331
Bbv12I GWGCWC 5 cut(s) 774, 945, 1443, 1495, 2181
BbvCI CCTCAGC 1 cut(s) 434
BccI CCATC 5 cut(s) 245, 392, 904, 908, 2209
BceAI ACGGC 5 cut(s) 508, 536, 598, 862, 1199
BciT130I CCWGG 1 cut(s) 1845
BcnI CCSGG 2 cut(s) 1037, 1075
BcoDI GTCTC 3 cut(s) 468, 1618, 2068
BfaI CTAG 3 cut(s) 1404, 2004, 2057
BfmI CTRYAG 3 cut(s) 1239, 1965, 2288
BfoI RGCGCY 3 cut(s) 444, 1507, 1647
BfuAI ACCTGC 1 cut(s) 2293
BglI GCCNNNNNGGC 2 cut(s) 213, 883
Bme1390I CCNGG 3 cut(s) 1037, 1075, 1845
Bme18I GGWCC 1 cut(s) 1751
BmeT110I CYCGRG 2 cut(s) 404, 592
BmgT120I GGNCC 5 cut(s) 368, 584, 722, 866, 1751
BmiI GGNNCC 4 cut(s) 256, 369, 442, 867
BmrFI CCNGG 3 cut(s) 1037, 1075, 1845
BmsI GCATC 4 cut(s) 1214, 1357, 2158, 2236
BmtI GCTAGC 1 cut(s) 1407
BpiI GAAGAC 2 cut(s) 2277, 2331
BplI GAGNNNNNCTC 2 cut(s) 1509, 1541
BpmI CTGGAG 3 cut(s) 461, 815, 1740
Bpu10I CCTNAGC 1 cut(s) 434
Bpu14I TTCGAA 2 cut(s) 1043, 1616
BpuEI CTTGAG 3 cut(s) 1427, 1625, 1850
BpuMI CCSGG 2 cut(s) 1037, 1075
BsaAI YACGTR 1 cut(s) 1903
BsaBI GATNNNNATC 1 cut(s) 1893
BsaHI GRCGYC 4 cut(s) 441, 915, 1180, 1686
BsaI GGTCTC 1 cut(s) 2068
BsaJI CCNNGG 4 cut(s) 211, 814, 1036, 1215
BsaWI WCCGGW 1 cut(s) 806
BsaXI ACNNNNNCTCC 4 cut(s) 407, 437, 1212, 1242
Bsc4I CCNNNNNNNGG 5 cut(s) 364, 530, 1157, 1314, 1609
Bse118I RCCGGY 2 cut(s) 935, 1294
Bse1I ACTGG 1 cut(s) 1340
Bse3DI GCAATG 2 cut(s) 970, 1654
Bse8I GATNNNNATC 1 cut(s) 1893
BseBI CCWGG 1 cut(s) 1845
BseDI CCNNGG 4 cut(s) 211, 814, 1036, 1215
BseGI GGATG 4 cut(s) 486, 896, 1372, 2173
BseJI GATNNNNATC 1 cut(s) 1893
BseLI CCNNNNNNNGG 5 cut(s) 364, 530, 1157, 1314, 1609
BseMI GCAATG 2 cut(s) 970, 1654
BseMII CTCAG 6 cut(s) 78, 89, 448, 571, 634, 1613
BseNI ACTGG 1 cut(s) 1340
BseRI GAGGAG 4 cut(s) 38, 566, 1451, 2170
BseSI GKGCMC 2 cut(s) 1495, 2181
BseYI CCCAGC 3 cut(s) 225, 529, 1396
BsgI GTGCAG 2 cut(s) 190, 1965
Bsh1236I CGCG 1 cut(s) 792
Bsh1285I CGRYCG 1 cut(s) 496
BshFI GGCC 8 cut(s) 370, 513, 551, 585, 605, 724, 868, 2260
BshNI GGYRCC 1 cut(s) 440
BsiEI CGRYCG 1 cut(s) 496
BsiHKAI GWGCWC 5 cut(s) 774, 945, 1443, 1495, 2181
BsiHKCI CYCGRG 2 cut(s) 404, 592
BsiSI CCGG 7 cut(s) 654, 807, 921, 936, 1036, 1074, 1295
BslFI GGGAC 3 cut(s) 1025, 1290, 1975
BslI CCNNNNNNNGG 5 cut(s) 364, 530, 1157, 1314, 1609
BsmAI GTCTC 3 cut(s) 468, 1618, 2068
BsmBI CGTCTC 1 cut(s) 468
BsmFI GGGAC 3 cut(s) 1025, 1290, 1975
BsmI GAATGC 2 cut(s) 1995, 2192
BsnI GGCC 8 cut(s) 370, 513, 551, 585, 605, 724, 868, 2260
Bso31I GGTCTC 1 cut(s) 2068
BsoBI CYCGRG 2 cut(s) 404, 592
Bsp119I TTCGAA 2 cut(s) 1043, 1616
Bsp1286I GDGCHC 5 cut(s) 774, 945, 1443, 1495, 2181
Bsp1407I TGTACA 1 cut(s) 1281
Bsp143I GATC 3 cut(s) 1096, 1888, 1894
BspANI GGCC 8 cut(s) 370, 513, 551, 585, 605, 724, 868, 2260
BspCNI CTCAG 6 cut(s) 77, 88, 447, 570, 633, 1614
BspFNI CGCG 1 cut(s) 792
BspHI TCATGA 1 cut(s) 1183
BspLI GGNNCC 4 cut(s) 256, 369, 442, 867
BspMAI CTGCAG 3 cut(s) 1243, 1969, 2292
BspMI ACCTGC 1 cut(s) 2293
BspOI GCTAGC 1 cut(s) 1407
BspPI GGATC 1 cut(s) 1896
BspT104I TTCGAA 2 cut(s) 1043, 1616
BspT107I GGYRCC 1 cut(s) 440
BspTNI GGTCTC 1 cut(s) 2068
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 970, 1654
BsrFI RCCGGY 2 cut(s) 935, 1294
BsrGI TGTACA 1 cut(s) 1281
BsrI ACTGG 1 cut(s) 1340
BssAI RCCGGY 2 cut(s) 935, 1294
BssECI CCNNGG 4 cut(s) 211, 814, 1036, 1215
BssMI GATC 3 cut(s) 1096, 1888, 1894
BssNI GRCGYC 4 cut(s) 441, 915, 1180, 1686
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 2 cut(s) 211, 814
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 1845
Bst4CI ACNGT 2 cut(s) 1957, 2330
BstACI GRCGYC 4 cut(s) 441, 915, 1180, 1686
BstAPI GCANNNNNTGC 1 cut(s) 1211
BstAUI TGTACA 1 cut(s) 1281
BstBAI YACGTR 1 cut(s) 1903
BstBI TTCGAA 2 cut(s) 1043, 1616
BstC8I GCNNGC 6 cut(s) 740, 941, 975, 1405, 1578, 2351
BstDEI CTNAG 7 cut(s) 64, 75, 434, 557, 620, 1622, 1733
BstDSI CCRYGG 1 cut(s) 1215
BstEII GGTNACC 1 cut(s) 760
BstF5I GGATG 4 cut(s) 486, 896, 1372, 2173
BstFNI CGCG 1 cut(s) 792
BstH2I RGCGCY 3 cut(s) 444, 1507, 1647
BstHHI GCGC 5 cut(s) 443, 1506, 1646, 1780, 2302
BstKTI GATC 3 cut(s) 1099, 1891, 1897
BstMAI GTCTC 3 cut(s) 468, 1618, 2068
BstMBI GATC 3 cut(s) 1096, 1888, 1894
BstMCI CGRYCG 1 cut(s) 496
BstMWI GCNNNNNNNGC 9 cut(s) 213, 314, 535, 835, 874, 883, 892, 1211, 1262
BstNI CCWGG 1 cut(s) 1845
BstNSI RCATGY 2 cut(s) 691, 958
BstPI GGTNACC 1 cut(s) 760
BstSCI CCNGG 3 cut(s) 1035, 1073, 1843
BstSFI CTRYAG 3 cut(s) 1239, 1965, 2288
BstSLI GKGCMC 2 cut(s) 1495, 2181
BstUI CGCG 1 cut(s) 792
BstV2I GAAGAC 2 cut(s) 2277, 2331
BstXI CCANNNNNNTGG 1 cut(s) 239
BsuRI GGCC 8 cut(s) 370, 513, 551, 585, 605, 724, 868, 2260
BtgI CCRYGG 1 cut(s) 1215
BtgZI GCGATG 1 cut(s) 370
BtsCI GGATG 4 cut(s) 486, 896, 1372, 2173
BtsI GCAGTG 1 cut(s) 2181
BtsIMutI CAGTG 2 cut(s) 150, 2181
BveI ACCTGC 1 cut(s) 2293
Cac8I GCNNGC 6 cut(s) 740, 941, 975, 1405, 1578, 2351
CaiI CAGNNNCTG 2 cut(s) 112, 1825
CciI TCATGA 1 cut(s) 1183
CfoI GCGC 5 cut(s) 443, 1506, 1646, 1780, 2302
Cfr10I RCCGGY 2 cut(s) 935, 1294
Cfr13I GGNCC 5 cut(s) 368, 584, 722, 866, 1751
CseI GACGC 3 cut(s) 781, 1169, 1675
Csp6I GTAC 3 cut(s) 1282, 1666, 2120
CspCI CAANNNNNGTGG 2 cut(s) 1197, 1232
CviAII CATG 7 cut(s) 688, 955, 1184, 1261, 1496, 2015, 2083
CviQI GTAC 3 cut(s) 1282, 1666, 2120
DdeI CTNAG 7 cut(s) 64, 75, 434, 557, 620, 1622, 1733
DinI GGCGCC 1 cut(s) 442
DpnI GATC 3 cut(s) 1098, 1890, 1896
DpnII GATC 3 cut(s) 1096, 1888, 1894
EaeI YGGCCR 1 cut(s) 549
EciI GGCGGA 1 cut(s) 261
Ecl136II GAGCTC 1 cut(s) 1441
Eco130I CCWWGG 2 cut(s) 211, 814
Eco147I AGGCCT 1 cut(s) 2260
Eco24I GRGCYC 1 cut(s) 1443
Eco31I GGTCTC 1 cut(s) 2068
Eco47I GGWCC 1 cut(s) 1751
Eco47III AGCGCT 1 cut(s) 1645
Eco53kI GAGCTC 1 cut(s) 1441
Eco57I CTGAAG 2 cut(s) 2225, 2247
Eco72I CACGTG 1 cut(s) 1903
Eco88I CYCGRG 2 cut(s) 404, 592
Eco91I GGTNACC 1 cut(s) 760
EcoICRI GAGCTC 1 cut(s) 1441
EcoO109I RGGNCCY 1 cut(s) 866
EcoO65I GGTNACC 1 cut(s) 760
EcoRI GAATTC 1 cut(s) 857
EcoRII CCWGG 1 cut(s) 1843
EcoT14I CCWWGG 2 cut(s) 211, 814
EcoT38I GRGCYC 1 cut(s) 1443
EgeI GGCGCC 1 cut(s) 442
EheI GGCGCC 1 cut(s) 442
ErhI CCWWGG 2 cut(s) 211, 814
Esp3I CGTCTC 1 cut(s) 468
FaeI CATG 7 cut(s) 691, 958, 1187, 1264, 1499, 2018, 2086
FalI AAGNNNNNCTT 4 cut(s) 727, 759, 2262, 2294
FaqI GGGAC 3 cut(s) 1025, 1290, 1975
FatI CATG 7 cut(s) 687, 954, 1183, 1260, 1495, 2014, 2082
FauI CCCGC 1 cut(s) 528
FokI GGATG 4 cut(s) 473, 883, 1379, 2180
FriOI GRGCYC 1 cut(s) 1443
FspBI CTAG 3 cut(s) 1404, 2004, 2057
GlaI GCGC 5 cut(s) 442, 1505, 1645, 1779, 2301
GsaI CCCAGC 3 cut(s) 229, 533, 1400
GsuI CTGGAG 3 cut(s) 461, 815, 1740
HaeII RGCGCY 3 cut(s) 444, 1507, 1647
HaeIII GGCC 8 cut(s) 370, 513, 551, 585, 605, 724, 868, 2260
HapII CCGG 7 cut(s) 654, 807, 921, 936, 1036, 1074, 1295
HgaI GACGC 3 cut(s) 781, 1169, 1675
HhaI GCGC 5 cut(s) 443, 1506, 1646, 1780, 2302
Hin1I GRCGYC 4 cut(s) 441, 915, 1180, 1686
Hin1II CATG 7 cut(s) 691, 958, 1187, 1264, 1499, 2018, 2086
Hin6I GCGC 5 cut(s) 441, 1504, 1644, 1778, 2300
HinP1I GCGC 5 cut(s) 441, 1504, 1644, 1778, 2300
HincII GTYRAC 4 cut(s) 36, 472, 1345, 2011
HindII GTYRAC 4 cut(s) 36, 472, 1345, 2011
HindIII AAGCTT 2 cut(s) 736, 836
HpaI GTTAAC 1 cut(s) 2011
HpaII CCGG 7 cut(s) 654, 807, 921, 936, 1036, 1074, 1295
HphI GGTGA 5 cut(s) 214, 925, 1172, 1329, 2355
Hpy166II GTNNAC 8 cut(s) 36, 58, 472, 1144, 1345, 1493, 2011, 2179
Hpy8I GTNNAC 8 cut(s) 36, 58, 472, 1144, 1345, 1493, 2011, 2179
Hpy99I CGWCG 7 cut(s) 529, 547, 797, 883, 920, 1535, 2033
HpyCH4III ACNGT 2 cut(s) 1957, 2330
HpyCH4IV ACGT 7 cut(s) 219, 785, 915, 1424, 1475, 1902, 2031
HpyF10VI GCNNNNNNNGC 9 cut(s) 213, 314, 535, 835, 874, 883, 892, 1211, 1262
HpyF3I CTNAG 7 cut(s) 64, 75, 434, 557, 620, 1622, 1733
HpySE526I ACGT 7 cut(s) 219, 785, 915, 1424, 1475, 1902, 2031
Hsp92I GRCGYC 4 cut(s) 441, 915, 1180, 1686
Hsp92II CATG 7 cut(s) 691, 958, 1187, 1264, 1499, 2018, 2086
HspAI GCGC 5 cut(s) 441, 1504, 1644, 1778, 2300
KasI GGCGCC 1 cut(s) 440
KspAI GTTAAC 1 cut(s) 2011
Kzo9I GATC 3 cut(s) 1096, 1888, 1894
LmnI GCTCC 8 cut(s) 234, 254, 777, 834, 1400, 1438, 1530, 2157
LweI GCATC 4 cut(s) 1214, 1357, 2158, 2236
MaeI CTAG 3 cut(s) 1404, 2004, 2057
MaeII ACGT 7 cut(s) 219, 785, 915, 1424, 1475, 1902, 2031
MaeIII GTNAC 4 cut(s) 220, 760, 1160, 1476
MalI GATC 3 cut(s) 1098, 1890, 1896
MbiI CCGCTC 1 cut(s) 308
MboI GATC 3 cut(s) 1096, 1888, 1894
MhlI GDGCHC 5 cut(s) 774, 945, 1443, 1495, 2181
Mly113I GGCGCC 1 cut(s) 441
MlyI GAGTC 4 cut(s) 92, 354, 475, 633
MmeI TCCRAC 3 cut(s) 382, 450, 775
MroXI GAANNNNTTC 3 cut(s) 145, 2064, 2274
MseI TTAA 2 cut(s) 1269, 2010
MslI CAYNNNNRTG 2 cut(s) 686, 938
MspA1I CMGCKG 6 cut(s) 68, 112, 271, 277, 438, 2252
MspI CCGG 7 cut(s) 654, 807, 921, 936, 1036, 1074, 1295
MspR9I CCNGG 3 cut(s) 1037, 1075, 1845
Mva1269I GAATGC 2 cut(s) 1995, 2192
MvaI CCWGG 1 cut(s) 1845
MvnI CGCG 1 cut(s) 792
MwoI GCNNNNNNNGC 9 cut(s) 213, 314, 535, 835, 874, 883, 892, 1211, 1262
NarI GGCGCC 1 cut(s) 441
NciI CCSGG 2 cut(s) 1037, 1075
NdeII GATC 3 cut(s) 1096, 1888, 1894
NheI GCTAGC 1 cut(s) 1403
NlaIII CATG 7 cut(s) 691, 958, 1187, 1264, 1499, 2018, 2086
NlaIV GGNNCC 4 cut(s) 256, 369, 442, 867
NmuCI GTSAC 2 cut(s) 220, 1160
NspI RCATGY 2 cut(s) 691, 958
NspV TTCGAA 2 cut(s) 1043, 1616
OliI CACNNNNGTG 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 1183
PceI AGGCCT 1 cut(s) 2260
PciI ACATGT 1 cut(s) 954
PcsI WCGNNNNNNNCGW 2 cut(s) 542, 1899
PctI GAATGC 2 cut(s) 1995, 2192
PdmI GAANNNNTTC 3 cut(s) 145, 2064, 2274
PflMI CCANNNNNTGG 1 cut(s) 1157
PfoI TCCNGGA 1 cut(s) 1073
PleI GAGTC 4 cut(s) 91, 354, 475, 632
PluTI GGCGCC 1 cut(s) 444
PmaCI CACGTG 1 cut(s) 1903
PmlI CACGTG 1 cut(s) 1903
PpsI GAGTC 4 cut(s) 91, 354, 475, 632
Ppu21I YACGTR 1 cut(s) 1903
PscI ACATGT 1 cut(s) 954
Psp124BI GAGCTC 1 cut(s) 1443
Psp1406I AACGTT 2 cut(s) 1424, 1475
Psp6I CCWGG 1 cut(s) 1843
PspCI CACGTG 1 cut(s) 1903
PspEI GGTNACC 1 cut(s) 760
PspFI CCCAGC 3 cut(s) 225, 529, 1396
PspGI CCWGG 1 cut(s) 1843
PspN4I GGNNCC 4 cut(s) 256, 369, 442, 867
PspPI GGNCC 5 cut(s) 368, 584, 722, 866, 1751
PstI CTGCAG 3 cut(s) 1243, 1969, 2292
PstNI CAGNNNCTG 2 cut(s) 112, 1825
PvuII CAGCTG 3 cut(s) 68, 112, 2252
RsaI GTAC 3 cut(s) 1283, 1667, 2121
RsaNI GTAC 3 cut(s) 1282, 1666, 2120
RseI CAYNNNNRTG 2 cut(s) 686, 938
SacI GAGCTC 1 cut(s) 1443
SaqAI TTAA 2 cut(s) 1269, 2010
Sau3AI GATC 3 cut(s) 1096, 1888, 1894
Sau96I GGNCC 5 cut(s) 368, 584, 722, 866, 1751
SchI GAGTC 4 cut(s) 92, 354, 475, 633
ScrFI CCNGG 3 cut(s) 1037, 1075, 1845
SduI GDGCHC 5 cut(s) 774, 945, 1443, 1495, 2181
SfaNI GCATC 4 cut(s) 1214, 1357, 2158, 2236
SfcI CTRYAG 3 cut(s) 1239, 1965, 2288
SfoI GGCGCC 1 cut(s) 442
Sfr274I CTCGAG 1 cut(s) 404
SfuI TTCGAA 2 cut(s) 1043, 1616
SgrAI CRCCGGYG 1 cut(s) 935
SinI GGWCC 1 cut(s) 1751
SlaI CTCGAG 1 cut(s) 404
SmiMI CAYNNNNRTG 2 cut(s) 686, 938
SmlI CTYRAG 4 cut(s) 404, 1442, 1604, 1865
SmoI CTYRAG 4 cut(s) 404, 1442, 1604, 1865
SseBI AGGCCT 1 cut(s) 2260
SspDI GGCGCC 1 cut(s) 440
SspI AATATT 1 cut(s) 1698
SspMI CTAG 3 cut(s) 1404, 2004, 2057
SstI GAGCTC 1 cut(s) 1443
StuI AGGCCT 1 cut(s) 2260
StyD4I CCNGG 3 cut(s) 1035, 1073, 1843
StyI CCWWGG 2 cut(s) 211, 814
TaaI ACNGT 2 cut(s) 1957, 2330
TaiI ACGT 7 cut(s) 222, 788, 918, 1427, 1478, 1905, 2034
TaqI TCGA 7 cut(s) 405, 492, 1043, 1420, 1464, 1616, 1710
TatI WGTACW 2 cut(s) 1281, 1665
TauI GCSGC 6 cut(s) 246, 260, 277, 317, 320, 441
Tru1I TTAA 2 cut(s) 1269, 2010
Tru9I TTAA 2 cut(s) 1269, 2010
TscAI CASTG 2 cut(s) 157, 2181
TseFI GTSAC 2 cut(s) 220, 1160
Tsp45I GTSAC 2 cut(s) 220, 1160
TspDTI ATGAA 8 cut(s) 870, 1143, 1249, 1406, 1732, 1988, 2109, 2349
TspGWI ACGGA 4 cut(s) 43, 531, 1179, 1548
TspRI CASTG 2 cut(s) 157, 2181
Van91I CCANNNNNTGG 1 cut(s) 1157
VneI GTGCAC 2 cut(s) 1491, 2177
VpaK11BI GGWCC 1 cut(s) 1751
XapI RAATTY 4 cut(s) 857, 929, 985, 1766
XceI RCATGY 2 cut(s) 691, 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 754
XhoI CTCGAG 1 cut(s) 404
XmnI GAANNNNTTC 3 cut(s) 145, 2064, 2274
XspI CTAG 3 cut(s) 1404, 2004, 2057
ZraI GACGTC 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.