pycom14g00530
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0001328
Physical Location & Seq
Forward (+)
358678 .. 366913
8236 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g00530.2

Sequence Viewer

Length: 2373 bp
ATGGCAAAACGCTCTCTCTGTTCTCTGCCTTTGGTTCTTGCTGTCATTATATTTTCAGTTCGTGATTCCGAACAGCACCAATCCTCCGAAGCTCTGACCCTTATGAGAATTACGCAACTCCTAAACTTTCCAACTGTCTCGAAAAGCTTGAATAACTACACAGATTTTTGCAACATTGAAGTGAATTCTTCTTTTGCGGTCTCATGCTACGAAGGGAACATAACCCAGCTGCATATAATCGGCGAAAAACCTGCTCCTTTGTTGCCTCGAAACTTTTCAATTGATTCCTTTGTAACAACTCTGGTTAAGCTTCCGAGCTTGAAAGTCCTCACATTGGTCTCTCTTGGTTTATGGGGAAACTTGCCTGGTAAAATCACACGTTTATCATCACTCGAAATACTTAATGTGACCTCAAATTTCTTGTATGGCACCATCCCTCCAGAGCTTTCCTCTCTAACAAGCCTCCAGACACTTATACTCGATGACAACATGTTTTCTGGACTGCTTCCAGAAGGGCTGGGTTCGCTACCAGTTTTATCTGTTTTGAGTTTGAAGAAGAACTTGTTCAATTCATCCTTGCCGAGTTCATTGAGTGAGTTGGAAAGTCTTAGACTTCTCGGGCTTTCTCATAACCACTTCTATGGGGAACTGCCTGATTTTAGTCGTTTGACGAACCTTCAAGTTCTTGAAGTGGGAGATAATGCTTTTGGACCTCAGTTTCCGAAACTTGGGAAAAAGTTGGTCACCCTTGTACTGAGAAAGAACAAATTTCGGTCTTCCATCCCTGCTGAATTGAGCTCCTATTATCAGCTACAATGTCTAGACGTTTCTTCCAACATGTTCGTGGGGCCATTTCCTCCATCGCTGCTATCTCTACCTTCGATTACTTACCTTAATTTTTCAGGAAATAAATTCACAGGAATGCTTTTCGAAAATTCATCTTGCAATGCTGAACTTAAAGTTGTGGATTTATCCTCGAATCTTTTGACTGGAAGCTTGCCAAAATGTCTTGAGTCGGATTCTAAGAACAAGGTTTTCCTGTATGCAAGCAATTGTTTAGCTACTAGTAATCAAAATCAACATCCGCTTCCATTTTGCCACAATGAGGCGTTAGCTGTTGGAATAGTACCTGACCGAAGCAAGCGAAAGCAAGCCTCCAAAGCAGCCCTTGCCTTGGGTATAATTGGAGCGCTTTTCGGATGTGTTCTATTTTTCGGTGTAATCTTCTTCGTATATAGAAGAATGAATGCCAACAAGACAACGAAGAAATCTCCAACAAGATCAATAACAGAGAATGCATCAAGTGGATATACCTCAAAGTTACTATCAGATGCAAGATATATATCTCAAACAATGAAGTCGGGAGCACTTGGCCTTCCAGGTTATCGAACCTTTTCACTCGAGGAACTTGAGGACGCTACACAAAACTTTGATACGTCTACTTTCATGGGTGAAGGCTCACATGGACAGATGTACAGAGGCCAGTTGAAGGATGGTTCTTTTGTTGCCATCAGATGCTTGAAAATGAAAGGAAGTCACAGCACTGAAAATTTCATGCACCATATAGAGCTAATTATGAAACTACGGCATCGCCATTTGGTCAGCGCACTTGGACACTGCTTTGAGTGTTACTTGGATGATTCGAGCGTCAGCAGAATATTTCTTGTCTTCGAATATGTACCAAATGGCACACTTCGGAGCTGGATATCTGGGGGACATCCCAGACGATCACTTGCGTGGACACAACGCATAGCAGCTGCAATAGGCATAGCAAAGGGCATTCAGTTTTTGCACACGGGGATCATGCCTGGTGTATTTTCAAATAATCTTAAAATAACAGACATTTTACTGGATCAGAATTTTGTTGCGAAAATTAGCAGTTACAACCTTCCTCTGTTAGAAGAAAATATGGAACAGATATGCTCCTCCTCGCCGGGTGGTCATGGAGTCTCTTCGAGCAGATGTGTAACTTCCAGTACCGGTGCAAGGAAGAAAGACGACGATAGGACTGATGTTCATAACTTTGGAGTAATATTGCTGGAAATGATCAAGGGGAGGCCGGTGAAGTCTGAGGCTCAAATCGAGGTTTTAGAAGATCAGCTACAAGTGGCCCTTACAGCTGATGATGAAGCTCGAAGAAGCGTGGTTGATCCACGGGTTAAGCAGACGTGCTTGGATCAGTCAGTGAAGACAATGATGGAAATTTGTGTTCGGTGTCTGCGCAAGGATCCACCAGAAAGGCCTTCTATTGACGATGTTCTGTGGAACTTGCAGTATGCTGAACAAGTTCAGGATGCATGGCAAGGAGGAGAATCCCAGAGCAGTGAGGGCTCTCCGGTTTCACCTTCACAACCTCCACGCCTCACCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

791

Amino Acids

87.15

Weight (kDa)

8.32

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2222
Acc36I ACCTGC 1 cut(s) 259
AccB1I GGYRCC 1 cut(s) 428
AccI GTMKAC 1 cut(s) 1439
AciI CCGC 2 cut(s) 197, 1085
AclWI GGATC 6 cut(s) 1808, 1860, 2144, 2184, 2222, 2235
AcsI RAATTY 8 cut(s) 184, 415, 767, 911, 934, 1549, 1858, 2202
AfaI GTAC 5 cut(s) 753, 1128, 1475, 1680, 1978
AfeI AGCGCT 1 cut(s) 1191
AfiI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 1986
AflIII ACRYGT 3 cut(s) 377, 489, 837
AgeI ACCGGT 1 cut(s) 1979
AhlI ACTAGT 1 cut(s) 1064
AjiI CACGTC 1 cut(s) 2169
AjnI CCWGG 3 cut(s) 364, 1378, 1807
AleI CACNNNNGTG 1 cut(s) 1735
Alw21I GWGCWC 2 cut(s) 800, 1369
Alw26I GTCTC 4 cut(s) 142, 205, 343, 1954
AlwI GGATC 6 cut(s) 1808, 1860, 2144, 2184, 2222, 2235
Ama87I CYCGRG 2 cut(s) 617, 1400
Aor51HI AGCGCT 1 cut(s) 1191
AoxI GGCC 6 cut(s) 848, 1372, 1480, 2057, 2109, 2240
ApeKI GCWGC 5 cut(s) 229, 865, 1163, 1754, 1757
ApoI RAATTY 8 cut(s) 184, 415, 767, 911, 934, 1549, 1858, 2202
AsiGI ACCGGT 1 cut(s) 1979
Asp700I GAANNNNTTC 4 cut(s) 274, 563, 1393, 2286
AspLEI GCGC 3 cut(s) 1192, 1607, 2223
AspS9I GGNCC 3 cut(s) 710, 848, 2110
AsuC2I CCSGG 1 cut(s) 1935
AsuHPI GGTGA 5 cut(s) 736, 1463, 2074, 2334, 2356
AsuII TTCGAA 2 cut(s) 930, 1671
AvaI CYCGRG 2 cut(s) 617, 1400
AvaII GGWCC 1 cut(s) 710
BamHI GGATCC 1 cut(s) 2227
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 2 cut(s) 800, 2333
BarI GAAGNNNNNNTAC 2 cut(s) 2085, 2117
BbsI GAAGAC 3 cut(s) 768, 1660, 2195
Bbv12I GWGCWC 2 cut(s) 800, 1369
BbvI GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BccI CCATC 6 cut(s) 440, 788, 868, 1487, 1517, 2191
BceAI ACGGC 1 cut(s) 1601
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 3 cut(s) 366, 1380, 1809
BclI TGATCA 1 cut(s) 2046
BcnI CCSGG 1 cut(s) 1935
BcoDI GTCTC 4 cut(s) 142, 205, 343, 1954
BcuI ACTAGT 1 cut(s) 1064
BfaI CTAG 2 cut(s) 821, 1065
BfoI RGCGCY 1 cut(s) 1193
BfuAI ACCTGC 1 cut(s) 259
BisI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
BlsI GCNGC 5 cut(s) 231, 867, 1165, 1756, 1759
Bme1390I CCNGG 4 cut(s) 366, 1380, 1809, 1935
Bme18I GGWCC 1 cut(s) 710
BmeT110I CYCGRG 2 cut(s) 617, 1400
BmgBI CACGTC 1 cut(s) 2169
BmgT120I GGNCC 3 cut(s) 710, 848, 2110
BmiI GGNNCC 3 cut(s) 430, 849, 2229
BmrFI CCNGG 4 cut(s) 366, 1380, 1809, 1935
BmsI GCATC 5 cut(s) 1307, 1321, 1505, 1597, 2284
BpiI GAAGAC 3 cut(s) 768, 1660, 2195
BplI GAGNNNNNCTC 2 cut(s) 434, 466
BpmI CTGGAG 2 cut(s) 423, 449
Bpu14I TTCGAA 2 cut(s) 930, 1671
BpuEI CTTGAG 2 cut(s) 1031, 1430
BpuMI CCSGG 1 cut(s) 1935
BsaBI GATNNNNATC 1 cut(s) 1342
BsaI GGTCTC 2 cut(s) 205, 343
BsaJI CCNNGG 2 cut(s) 1173, 2153
BsaWI WCCGGW 2 cut(s) 1979, 2335
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 421, 451
Bsc4I CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 1986
Bse118I RCCGGY 2 cut(s) 1979, 2059
Bse1I ACTGG 5 cut(s) 530, 994, 1483, 1854, 1974
Bse3DI GCAATG 1 cut(s) 952
Bse8I GATNNNNATC 1 cut(s) 1342
BseBI CCWGG 3 cut(s) 366, 1380, 1809
BseDI CCNNGG 2 cut(s) 1173, 2153
BseGI GGATG 9 cut(s) 432, 572, 780, 1081, 1205, 1498, 1642, 1717, 2299
BseJI GATNNNNATC 1 cut(s) 1342
BseLI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 1986
BseMI GCAATG 1 cut(s) 952
BseMII CTCAG 3 cut(s) 728, 746, 2061
BseNI ACTGG 5 cut(s) 530, 994, 1483, 1854, 1974
BseRI GAGGAG 3 cut(s) 1915, 1918, 2322
BseXI GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BseYI CCCAGC 2 cut(s) 225, 517
BshFI GGCC 6 cut(s) 850, 1374, 1482, 2059, 2111, 2242
BshNI GGYRCC 1 cut(s) 428
BshTI ACCGGT 1 cut(s) 1979
BsiHKAI GWGCWC 2 cut(s) 800, 1369
BsiHKCI CYCGRG 2 cut(s) 617, 1400
BsiSI CCGG 4 cut(s) 1934, 1980, 2060, 2336
BslFI GGGAC 1 cut(s) 1728
BslI CCNNNNNNNGG 6 cut(s) 334, 728, 1105, 1174, 1489, 1986
BsmAI GTCTC 4 cut(s) 142, 205, 343, 1954
BsmFI GGGAC 1 cut(s) 1728
BsmI GAATGC 4 cut(s) 927, 1252, 1300, 1779
BsnI GGCC 6 cut(s) 850, 1374, 1482, 2059, 2111, 2242
Bso31I GGTCTC 2 cut(s) 205, 343
BsoBI CYCGRG 2 cut(s) 617, 1400
Bsp119I TTCGAA 2 cut(s) 930, 1671
Bsp1286I GDGCHC 3 cut(s) 800, 1369, 2333
Bsp1407I TGTACA 1 cut(s) 1473
Bsp143I GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
BspACI CCGC 2 cut(s) 197, 1085
BspANI GGCC 6 cut(s) 850, 1374, 1482, 2059, 2111, 2242
BspCNI CTCAG 3 cut(s) 727, 747, 2062
BspLI GGNNCC 3 cut(s) 430, 849, 2229
BspMI ACCTGC 1 cut(s) 259
BspPI GGATC 6 cut(s) 1808, 1860, 2144, 2184, 2222, 2235
BspT104I TTCGAA 2 cut(s) 930, 1671
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 2 cut(s) 205, 343
BsrDI GCAATG 1 cut(s) 952
BsrFI RCCGGY 2 cut(s) 1979, 2059
BsrGI TGTACA 1 cut(s) 1473
BsrI ACTGG 5 cut(s) 530, 994, 1483, 1854, 1974
BssAI RCCGGY 2 cut(s) 1979, 2059
BssECI CCNNGG 2 cut(s) 1173, 2153
BssMI GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
BssT1I CCWWGG 1 cut(s) 1173
Bst2UI CCWGG 3 cut(s) 366, 1380, 1809
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 1957
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstAUI TGTACA 1 cut(s) 1473
BstBI TTCGAA 2 cut(s) 930, 1671
BstC8I GCNNGC 4 cut(s) 998, 1048, 1142, 1152
BstDEI CTNAG 5 cut(s) 608, 714, 755, 1023, 2070
BstDSI CCRYGG 1 cut(s) 2153
BstEII GGTNACC 1 cut(s) 742
BstF5I GGATG 9 cut(s) 432, 572, 780, 1081, 1205, 1498, 1642, 1717, 2299
BstH2I RGCGCY 1 cut(s) 1193
BstHHI GCGC 3 cut(s) 1192, 1607, 2223
BstKTI GATC 9 cut(s) 1283, 1730, 1803, 1855, 2049, 2098, 2152, 2179, 2230
BstMAI GTCTC 4 cut(s) 142, 205, 343, 1954
BstMBI GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
BstMWI GCNNNNNNNGC 5 cut(s) 523, 1160, 1169, 2117, 2328
BstNI CCWGG 3 cut(s) 366, 1380, 1809
BstNSI RCATGY 2 cut(s) 493, 841
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 4 cut(s) 364, 1378, 1807, 1933
BstV1I GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
BstV2I GAAGAC 3 cut(s) 768, 1660, 2195
BstX2I RGATCY 1 cut(s) 2227
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 1 cut(s) 2227
BsuRI GGCC 6 cut(s) 850, 1374, 1482, 2059, 2111, 2242
BtgI CCRYGG 1 cut(s) 2153
BtgZI GCGATG 2 cut(s) 846, 1574
BtrI CACGTC 1 cut(s) 2169
BtsCI GGATG 9 cut(s) 432, 572, 780, 1081, 1205, 1498, 1642, 1717, 2299
BtsI GCAGTG 2 cut(s) 1615, 2329
BtsIMutI CAGTG 4 cut(s) 1542, 1615, 2190, 2329
BveI ACCTGC 1 cut(s) 259
Cac8I GCNNGC 4 cut(s) 998, 1048, 1142, 1152
CfoI GCGC 3 cut(s) 1192, 1607, 2223
Cfr10I RCCGGY 2 cut(s) 1979, 2059
Cfr13I GGNCC 3 cut(s) 710, 848, 2110
CseI GACGC 2 cut(s) 1424, 1636
Csp6I GTAC 5 cut(s) 752, 1127, 1474, 1679, 1977
CspAI ACCGGT 1 cut(s) 1979
CviAII CATG 9 cut(s) 204, 490, 838, 1447, 1463, 1555, 1804, 1943, 2298
CviQI GTAC 5 cut(s) 752, 1127, 1474, 1679, 1977
DdeI CTNAG 5 cut(s) 608, 714, 755, 1023, 2070
DpnI GATC 9 cut(s) 1282, 1729, 1802, 1854, 2048, 2097, 2151, 2178, 2229
DpnII GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
Eam1104I CTCTTC 1 cut(s) 1957
EarI CTCTTC 1 cut(s) 1957
Ecl136II GAGCTC 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 1173
Eco147I AGGCCT 1 cut(s) 2242
Eco24I GRGCYC 2 cut(s) 800, 2333
Eco31I GGTCTC 2 cut(s) 205, 343
Eco32I GATATC 1 cut(s) 1707
Eco47I GGWCC 1 cut(s) 710
Eco47III AGCGCT 1 cut(s) 1191
Eco53kI GAGCTC 1 cut(s) 798
Eco88I CYCGRG 2 cut(s) 617, 1400
Eco91I GGTNACC 1 cut(s) 742
EcoICRI GAGCTC 1 cut(s) 798
EcoO65I GGTNACC 1 cut(s) 742
EcoRI GAATTC 1 cut(s) 184
EcoRII CCWGG 3 cut(s) 364, 1378, 1807
EcoRV GATATC 1 cut(s) 1707
EcoT14I CCWWGG 1 cut(s) 1173
EcoT22I ATGCAT 2 cut(s) 1300, 2299
EcoT38I GRGCYC 2 cut(s) 800, 2333
ErhI CCWWGG 1 cut(s) 1173
FaeI CATG 9 cut(s) 207, 493, 841, 1450, 1466, 1558, 1807, 1946, 2301
FalI AAGNNNNNCTT 6 cut(s) 545, 577, 1152, 1184, 2097, 2129
FaqI GGGAC 1 cut(s) 1728
FatI CATG 9 cut(s) 203, 489, 837, 1446, 1462, 1554, 1803, 1942, 2297
FbaI TGATCA 1 cut(s) 2046
FblI GTMKAC 1 cut(s) 1439
Fnu4HI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
FokI GGATG 9 cut(s) 419, 559, 767, 1068, 1212, 1505, 1649, 1704, 2306
FriOI GRGCYC 2 cut(s) 800, 2333
Fsp4HI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
FspBI CTAG 2 cut(s) 821, 1065
FspI TGCGCA 1 cut(s) 2222
GlaI GCGC 3 cut(s) 1191, 1606, 2222
GluI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
GsaI CCCAGC 2 cut(s) 229, 521
GsuI CTGGAG 2 cut(s) 423, 449
HaeII RGCGCY 1 cut(s) 1193
HaeIII GGCC 6 cut(s) 850, 1374, 1482, 2059, 2111, 2242
HapII CCGG 4 cut(s) 1934, 1980, 2060, 2336
HgaI GACGC 2 cut(s) 1424, 1636
HhaI GCGC 3 cut(s) 1192, 1607, 2223
Hin1II CATG 9 cut(s) 207, 493, 841, 1450, 1466, 1558, 1807, 1946, 2301
Hin6I GCGC 3 cut(s) 1190, 1605, 2221
HinP1I GCGC 3 cut(s) 1190, 1605, 2221
HindIII AAGCTT 3 cut(s) 145, 308, 994
HinfI GANTC 8 cut(s) 65, 284, 979, 1013, 1019, 1640, 1947, 2312
HpaII CCGG 4 cut(s) 1934, 1980, 2060, 2336
HphI GGTGA 5 cut(s) 736, 1463, 2074, 2334, 2356
Hpy166II GTNNAC 2 cut(s) 1440, 1740
Hpy8I GTNNAC 2 cut(s) 1440, 1740
Hpy99I CGWCG 1 cut(s) 2003
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 4 cut(s) 379, 825, 1436, 2168
HpyF10VI GCNNNNNNNGC 5 cut(s) 523, 1160, 1169, 2117, 2328
HpyF3I CTNAG 5 cut(s) 608, 714, 755, 1023, 2070
HpySE526I ACGT 4 cut(s) 379, 825, 1436, 2168
Hsp92II CATG 9 cut(s) 207, 493, 841, 1450, 1466, 1558, 1807, 1946, 2301
HspAI GCGC 3 cut(s) 1190, 1605, 2221
Ksp22I TGATCA 1 cut(s) 2046
Kzo9I GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
LmnI GCTCC 6 cut(s) 259, 803, 1187, 1364, 1698, 1928
Lsp1109I GCAGC 5 cut(s) 216, 852, 1175, 1744, 1766
LweI GCATC 5 cut(s) 1307, 1321, 1505, 1597, 2284
MaeI CTAG 2 cut(s) 821, 1065
MaeII ACGT 4 cut(s) 379, 825, 1436, 2168
MaeIII GTNAC 8 cut(s) 292, 406, 742, 1320, 1535, 1628, 1880, 1966
MalI GATC 9 cut(s) 1282, 1729, 1802, 1854, 2048, 2097, 2151, 2178, 2229
MboI GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
MfeI CAATTG 2 cut(s) 279, 1051
MflI RGATCY 1 cut(s) 2227
MhlI GDGCHC 3 cut(s) 800, 1369, 2333
MlyI GAGTC 2 cut(s) 1022, 1956
MmeI TCCRAC 6 cut(s) 155, 579, 858, 996, 1099, 1298
Mph1103I ATGCAT 2 cut(s) 1300, 2299
MroXI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2286
MseI TTAA 6 cut(s) 306, 402, 894, 957, 1830, 2160
MslI CAYNNNNRTG 5 cut(s) 179, 639, 842, 920, 1735
MspA1I CMGCKG 3 cut(s) 229, 1757, 2120
MspI CCGG 4 cut(s) 1934, 1980, 2060, 2336
MspR9I CCNGG 4 cut(s) 366, 1380, 1809, 1935
MunI CAATTG 2 cut(s) 279, 1051
Mva1269I GAATGC 4 cut(s) 927, 1252, 1300, 1779
MvaI CCWGG 3 cut(s) 366, 1380, 1809
MwoI GCNNNNNNNGC 5 cut(s) 523, 1160, 1169, 2117, 2328
NciI CCSGG 1 cut(s) 1935
NdeII GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
NlaIII CATG 9 cut(s) 207, 493, 841, 1450, 1466, 1558, 1807, 1946, 2301
NlaIV GGNNCC 3 cut(s) 430, 849, 2229
NmeAIII GCCGAG 1 cut(s) 606
NmuCI GTSAC 3 cut(s) 406, 742, 1535
NsbI TGCGCA 1 cut(s) 2222
NsiI ATGCAT 2 cut(s) 1300, 2299
NspI RCATGY 2 cut(s) 493, 841
NspV TTCGAA 2 cut(s) 930, 1671
OliI CACNNNNGTG 1 cut(s) 1735
PaeR7I CTCGAG 1 cut(s) 1400
PceI AGGCCT 1 cut(s) 2242
PciI ACATGT 2 cut(s) 489, 837
PctI GAATGC 4 cut(s) 927, 1252, 1300, 1779
PdmI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2286
PfeI GAWTC 6 cut(s) 65, 284, 979, 1019, 1640, 2312
PinAI ACCGGT 1 cut(s) 1979
PkrI GCNGC 5 cut(s) 231, 867, 1165, 1756, 1759
PleI GAGTC 2 cut(s) 1021, 1955
PpsI GAGTC 2 cut(s) 1021, 1955
PscI ACATGT 2 cut(s) 489, 837
Psp124BI GAGCTC 1 cut(s) 800
Psp6I CCWGG 3 cut(s) 364, 1378, 1807
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 2 cut(s) 225, 517
PspGI CCWGG 3 cut(s) 364, 1378, 1807
PspN4I GGNNCC 3 cut(s) 430, 849, 2229
PspPI GGNCC 3 cut(s) 710, 848, 2110
PspXI VCTCGAGB 1 cut(s) 1400
PsuI RGATCY 1 cut(s) 2227
PvuII CAGCTG 3 cut(s) 229, 1757, 2120
RsaI GTAC 5 cut(s) 753, 1128, 1475, 1680, 1978
RsaNI GTAC 5 cut(s) 752, 1127, 1474, 1679, 1977
RseI CAYNNNNRTG 5 cut(s) 179, 639, 842, 920, 1735
SacI GAGCTC 1 cut(s) 800
SaqAI TTAA 6 cut(s) 306, 402, 894, 957, 1830, 2160
SatI GCNGC 5 cut(s) 230, 866, 1164, 1755, 1758
Sau3AI GATC 9 cut(s) 1280, 1727, 1800, 1852, 2046, 2095, 2149, 2176, 2227
Sau96I GGNCC 3 cut(s) 710, 848, 2110
SchI GAGTC 2 cut(s) 1022, 1956
ScrFI CCNGG 4 cut(s) 366, 1380, 1809, 1935
SduI GDGCHC 3 cut(s) 800, 1369, 2333
SfaNI GCATC 5 cut(s) 1307, 1321, 1505, 1597, 2284
Sfr274I CTCGAG 1 cut(s) 1400
SfuI TTCGAA 2 cut(s) 930, 1671
SinI GGWCC 1 cut(s) 710
SlaI CTCGAG 1 cut(s) 1400
SmiMI CAYNNNNRTG 5 cut(s) 179, 639, 842, 920, 1735
SmlI CTYRAG 3 cut(s) 1010, 1400, 1409
SmoI CTYRAG 3 cut(s) 1010, 1400, 1409
SpeI ACTAGT 1 cut(s) 1064
SseBI AGGCCT 1 cut(s) 2242
SsiI CCGC 2 cut(s) 197, 1085
SspI AATATT 2 cut(s) 1659, 2034
SspMI CTAG 2 cut(s) 821, 1065
SstI GAGCTC 1 cut(s) 800
StuI AGGCCT 1 cut(s) 2242
StyD4I CCNGG 4 cut(s) 364, 1378, 1807, 1933
StyI CCWWGG 1 cut(s) 1173
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 4 cut(s) 382, 828, 1439, 2171
TaqII GACCGA 2 cut(s) 762, 1149
TatI WGTACW 2 cut(s) 751, 1473
TfiI GAWTC 6 cut(s) 65, 284, 979, 1019, 1640, 2312
Tru1I TTAA 6 cut(s) 306, 402, 894, 957, 1830, 2160
Tru9I TTAA 6 cut(s) 306, 402, 894, 957, 1830, 2160
TscAI CASTG 4 cut(s) 1549, 1622, 2190, 2329
TseFI GTSAC 3 cut(s) 406, 742, 1535
TseI GCWGC 5 cut(s) 229, 865, 1163, 1754, 1757
Tsp45I GTSAC 3 cut(s) 406, 742, 1535
TspRI CASTG 4 cut(s) 1549, 1622, 2190, 2329
VpaK11BI GGWCC 1 cut(s) 710
XapI RAATTY 8 cut(s) 184, 415, 767, 911, 934, 1549, 1858, 2202
XbaI TCTAGA 1 cut(s) 820
XceI RCATGY 2 cut(s) 493, 841
XcmI CCANNNNNNNNNTGG 2 cut(s) 841, 1490
XhoI CTCGAG 1 cut(s) 1400
XmiI GTMKAC 1 cut(s) 1439
XmnI GAANNNNTTC 4 cut(s) 274, 563, 1393, 2286
XspI CTAG 2 cut(s) 821, 1065
Zsp2I ATGCAT 2 cut(s) 1300, 2299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.