pycom02g22440
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001328
Physical Location & Seq
Forward (+)
20547625 .. 20551742
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g22440.3

Sequence Viewer

Length: 2199 bp
ATGGCGAACTCACATCTTCTTCTTCTTCTGTTGATATCCTCCCTCTTCTCCGTCCACCACTCAGGTGCTGCTACTGAGTCGGAGTCTGAGAGCCTTCTGAAAATCCAGCAGCTCCTCAACTACCCACCACCTCTAACCCTAGTCAGTTCCAAGGACGGACACTCTTTGTGCAGCATTGAACCAACTCCTTCCCTCACTCTCTCTTGCTACCAAGGCAACGTTACCCAGCTCCACATTTCAGGCGGCGGAGCTGCCTTCCCTCCGCTGCCGCGTGACTTCTCAACCCACTCTCTCTTCTCCGCTCTCGCCTCCGCCTTCCCAACCCTCAAAGTCCTATCTCTGGTCTCTCTCCGCCTCTGGGGCCAACTCCCTCCCTCCATCGCCGGTTTATCTTCCCTCGAGATTCTCAACCTCAGCTCCAACTACCTCAGCGGCGCCATCCCTCCCCACATTTCCTCCCTCGTCAACCTCCAGACTCTCATCCTCGACCGCAACAACTTCACCGGCCGACTCCCGCCGTCGCTGGCTTCGCTTCGGTCGTTGGCCGTTCTCAGTTTGAACCACAACCTCCTCAACGGCTTTCTCCCGCGTTCTCTGGCATCTCTGCAAACCCTCAGAGTCCTCTCTCTTTCCCACAACTTCCTGGCCGGCGAAGTTCCTGACCTCAAAAGCCTCACAAACTTGCAATTTCTTGATTTGGAAGCTAACTATTTCGGGCCTCGTTTTCCAAGCTTACGTTCCAAGAACAAGCTGGTAACCCTTGTGCTCCGAAACAACAAGTTTCGCCTTGGAATCCAGACCGGATTCCGAGGCGGTTTTCAGCTCCAGAGGCTTGACATTTCACTCAATCAATTCGTGGGGCCTTTTTCGCCTTCATGGCTGTCGCTGCCTTCCATCAATTATCTTGACATTGCCGGAAATAAATTCACCGGAGTGCTCTCCAAGAACATGTCTTGCAATGCTGAGCTTGCTTTTGTGAATTTGTCCTCAAATCTTTTGGCAGGGGACTTGCCCACTTGCCTCAAAAACCGGGGTTCGAAGAGCAGCAGTGTGGTTGTTCTCTATTCCGGGAATTGTTTGACGAATGTGGACCAGAAGCAGCAGCAGCATCCTTCTAACTTGTGCCACCATGAAGCTCTGGCGGTGGAGATACCGCCTTCTGGTGACGAAAAGCGCAAGAGGCTTCATGATAATAAACAAGTCGTTGCATCAAAAGCCCTGGCAGGCATTGTGGGTGCCGCTGCAATTGTTGTTCTGGCTTTCATGGCTGTTAAGTGGTTATACGGCGAACAGCTGCCTGTTAAAAGTCCCAGAACACGGTTGATTAGAGATTCAGCTTCAACAGTCAACACAGCCAAGCTACTCTCGGATGCAAAATATATATCAGAAACAATGAAGCTGGGAGCCAGCGTTCCTGCTTATCGAACATTTTCTTTGGAGGAGCTCAAGGAAGCTACGAATGACTTTGATGATTCAACATTACTTGGTGAAGGCAAACATGGACAGATGTATAGAGGGAAGCTCACCAATGGAACACTTGTTGCTATCAGAGGCTTGAAAATGAGAAAGAGGCAGGCTCCACAAGTCTACACGCACCACCTTGAGCTGATTTCAAAACTGAGACATAGCCATTTGGTTAGTGCTCTTGGACATTGCTTGGAGTACCATCCCGATGATTCAGGGGTCGGCAGAATATTTCTTGTATTTGAGTTTGTTCCAAATGGAACTCTAAGAGGCTGCATTTCTGAAGGACCTCCAGGACGAAAGCTTACTTGGGCGCAGAGAATAGTAGCTGCAATTGGAGTTGCAAAGGGTATTCAGTTTCTGCATACAGGGATTGTTCCTGGTGTAAAATCAAATAATCTCAAGATAACAAATGTCTTATTTGATCACGATCTTCATGTGAAAATAAGCAGTTATAACCTACCTCTTTTAGCTGAAAACAACAGGGGAACGGTGGGTACTACAGTTTCTTCTCCTGCACTGAAAGGAAGCATTCAAACTAGGGTTAACCATGAGTGTAAGAACGACGTTTATGATATTGGAGTAATCTTGCTAGAAATCATTTTGGGGAGGCCTATCATGTTCCAAAATGAAGTTGGTGTTTTGAAAGACCTTTTACAAGTAAGTTTGACCACTGATGACACAGCTCGAAGGAGCATTGATCGATCCAGCAGTGCACAAGGAATGCTCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

733

Amino Acids

79.56

Weight (kDa)

9.47

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1920
AccB1I GGYRCC 2 cut(s) 434, 1235
AccBSI CCGCTC 1 cut(s) 302
AccI GTMKAC 1 cut(s) 1587
AccII CGCG 2 cut(s) 271, 589
AclI AACGTT 1 cut(s) 219
AclWI GGATC 1 cut(s) 2163
AcoI YGGCCR 3 cut(s) 505, 543, 645
AcsI RAATTY 2 cut(s) 923, 979
AcuI CTGAAG 1 cut(s) 1767
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 2 cut(s) 1664, 1963
AfiI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1317
AflIII ACRYGT 1 cut(s) 948
AgsI TTSAA 8 cut(s) 179, 559, 1341, 1476, 1558, 1614, 2000, 2110
AjnI CCWGG 4 cut(s) 642, 1218, 1756, 1843
AjuI GAANNNNNNNTTGG 4 cut(s) 721, 753, 1756, 1788
AleI CACNNNNGTG 2 cut(s) 63, 932
AloI GAACNNNNNNTCC 2 cut(s) 1395, 1427
Alw21I GWGCWC 5 cut(s) 768, 939, 1446, 1645, 2182
Alw26I GTCTC 2 cut(s) 349, 1615
Alw44I GTGCAC 1 cut(s) 2178
AlwI GGATC 1 cut(s) 2163
AlwNI CAGNNNCTG 2 cut(s) 68, 1825
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 7 cut(s) 361, 505, 543, 645, 716, 860, 2075
ApaLI GTGCAC 1 cut(s) 2178
ApoI RAATTY 2 cut(s) 923, 979
Asp700I GAANNNNTTC 1 cut(s) 1429
AspLEI GCGC 3 cut(s) 437, 1176, 1780
AspS9I GGNCC 5 cut(s) 361, 716, 860, 1090, 1751
AsuC2I CCSGG 2 cut(s) 1031, 1069
AsuHPI GGTGA 5 cut(s) 493, 919, 1175, 1499, 1516
AsuII TTCGAA 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 2 cut(s) 1090, 1751
BaeGI GKGCMC 1 cut(s) 2182
BaeI ACNNNNGTAYC 2 cut(s) 1953, 1986
BanI GGYRCC 2 cut(s) 434, 1235
BanII GRGCYC 1 cut(s) 1446
Bbv12I GWGCWC 5 cut(s) 768, 939, 1446, 1645, 2182
BbvCI CCTCAGC 2 cut(s) 413, 428
BccI CCATC 4 cut(s) 386, 446, 902, 1674
BceAI ACGGC 4 cut(s) 502, 530, 592, 1300
BciT130I CCWGG 4 cut(s) 644, 1220, 1758, 1845
BclI TGATCA 1 cut(s) 1888
BcnI CCSGG 2 cut(s) 1031, 1069
BcoDI GTCTC 2 cut(s) 349, 1615
BfaI CTAG 3 cut(s) 140, 2004, 2057
BfmI CTRYAG 1 cut(s) 1965
BfoI RGCGCY 1 cut(s) 438
BglI GCCNNNNNGGC 2 cut(s) 360, 877
BlpI GCTNAGC 1 cut(s) 963
Bme1390I CCNGG 6 cut(s) 644, 1031, 1069, 1220, 1758, 1845
Bme18I GGWCC 2 cut(s) 1090, 1751
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 5 cut(s) 361, 716, 860, 1090, 1751
BmiI GGNNCC 6 cut(s) 362, 436, 861, 1237, 1405, 1578
BmrFI CCNGG 6 cut(s) 644, 1031, 1069, 1220, 1758, 1845
BmsI GCATC 4 cut(s) 608, 1117, 1217, 1360
BplI GAGNNNNNCTC 4 cut(s) 1506, 1538, 1561, 1593
BpmI CTGGAG 3 cut(s) 455, 809, 1740
Bpu10I CCTNAGC 2 cut(s) 413, 428
Bpu1102I GCTNAGC 1 cut(s) 963
Bpu14I TTCGAA 1 cut(s) 1037
BpuEI CTTGAG 3 cut(s) 1430, 1622, 1850
BpuMI CCSGG 2 cut(s) 1031, 1069
Bsa29I ATCGAT 1 cut(s) 2167
BsaBI GATNNNNATC 1 cut(s) 1893
BsaHI GRCGYC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 6 cut(s) 150, 211, 787, 808, 1030, 1218
BsaWI WCCGGW 2 cut(s) 800, 929
BsaXI ACNNNNNCTCC 6 cut(s) 401, 431, 440, 470, 1395, 1425
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1317
Bse118I RCCGGY 3 cut(s) 383, 503, 647
Bse3DI GCAATG 3 cut(s) 909, 964, 1651
Bse8I GATNNNNATC 1 cut(s) 1893
BseBI CCWGG 4 cut(s) 644, 1220, 1758, 1845
BseCI ATCGAT 1 cut(s) 2167
BseDI CCNNGG 6 cut(s) 150, 211, 787, 808, 1030, 1218
BseGI GGATG 5 cut(s) 438, 480, 1108, 1375, 1666
BseJI GATNNNNATC 1 cut(s) 1893
BseLI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1317
BseMI GCAATG 3 cut(s) 909, 964, 1651
BseMII CTCAG 9 cut(s) 66, 75, 78, 427, 442, 565, 628, 954, 1610
BseRI GAGGAG 3 cut(s) 104, 560, 1454
BseSI GKGCMC 1 cut(s) 2182
BseX3I CGGCCG 1 cut(s) 505
BseYI CCCAGC 2 cut(s) 225, 1399
BsgI GTGCAG 2 cut(s) 190, 1965
Bsh1236I CGCG 2 cut(s) 271, 589
Bsh1285I CGRYCG 3 cut(s) 490, 508, 539
BshFI GGCC 7 cut(s) 363, 507, 545, 647, 718, 862, 2077
BshNI GGYRCC 2 cut(s) 434, 1235
BshVI ATCGAT 1 cut(s) 2167
BsiEI CGRYCG 3 cut(s) 490, 508, 539
BsiHKAI GWGCWC 5 cut(s) 768, 939, 1446, 1645, 2182
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 8 cut(s) 384, 504, 648, 801, 915, 930, 1030, 1068
BslFI GGGAC 2 cut(s) 1019, 1293
BslI CCNNNNNNNGG 4 cut(s) 340, 358, 1160, 1317
BsmAI GTCTC 2 cut(s) 349, 1615
BsmFI GGGAC 2 cut(s) 1019, 1293
BsmI GAATGC 2 cut(s) 1995, 2193
BsnI GGCC 7 cut(s) 363, 507, 545, 647, 718, 862, 2077
Bso31I GGTCTC 1 cut(s) 349
BsoBI CYCGRG 1 cut(s) 398
Bsp119I TTCGAA 1 cut(s) 1037
Bsp1286I GDGCHC 5 cut(s) 768, 939, 1446, 1645, 2182
Bsp143I GATC 4 cut(s) 1888, 1894, 2164, 2168
Bsp1720I GCTNAGC 1 cut(s) 963
BspANI GGCC 7 cut(s) 363, 507, 545, 647, 718, 862, 2077
BspCNI CTCAG 9 cut(s) 67, 74, 79, 426, 441, 564, 627, 955, 1611
BspDI ATCGAT 1 cut(s) 2167
BspFNI CGCG 2 cut(s) 271, 589
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 6 cut(s) 362, 436, 861, 1237, 1405, 1578
BspPI GGATC 1 cut(s) 2163
BspQI GCTCTTC 1 cut(s) 1034
BspT104I TTCGAA 1 cut(s) 1037
BspT107I GGYRCC 2 cut(s) 434, 1235
BspTNI GGTCTC 1 cut(s) 349
BsrBI CCGCTC 1 cut(s) 302
BsrDI GCAATG 3 cut(s) 909, 964, 1651
BsrFI RCCGGY 3 cut(s) 383, 503, 647
BssAI RCCGGY 3 cut(s) 383, 503, 647
BssECI CCNNGG 6 cut(s) 150, 211, 787, 808, 1030, 1218
BssMI GATC 4 cut(s) 1888, 1894, 2164, 2168
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 3 cut(s) 150, 211, 787
Bst2UI CCWGG 4 cut(s) 644, 1220, 1758, 1845
Bst4CI ACNGT 4 cut(s) 1320, 1345, 1957, 1969
Bst6I CTCTTC 3 cut(s) 50, 299, 1034
BstACI GRCGYC 1 cut(s) 435
BstBI TTCGAA 1 cut(s) 1037
BstC8I GCNNGC 6 cut(s) 525, 649, 969, 1225, 1408, 1575
BstEII GGTNACC 1 cut(s) 754
BstF5I GGATG 5 cut(s) 438, 480, 1108, 1375, 1666
BstFNI CGCG 2 cut(s) 271, 589
BstH2I RGCGCY 1 cut(s) 438
BstHHI GCGC 3 cut(s) 437, 1176, 1780
BstKTI GATC 4 cut(s) 1891, 1897, 2167, 2171
BstMAI GTCTC 2 cut(s) 349, 1615
BstMBI GATC 4 cut(s) 1888, 1894, 2164, 2168
BstMCI CGRYCG 3 cut(s) 490, 508, 539
BstNI CCWGG 4 cut(s) 644, 1220, 1758, 1845
BstNSI RCATGY 1 cut(s) 952
BstPI GGTNACC 1 cut(s) 754
BstSCI CCNGG 6 cut(s) 642, 1029, 1067, 1218, 1756, 1843
BstSFI CTRYAG 1 cut(s) 1965
BstSLI GKGCMC 1 cut(s) 2182
BstUI CGCG 2 cut(s) 271, 589
BstZI CGGCCG 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 2167
BsuRI GGCC 7 cut(s) 363, 507, 545, 647, 718, 862, 2077
BsuTUI ATCGAT 1 cut(s) 2167
BtgZI GCGATG 1 cut(s) 364
BtsCI GGATG 5 cut(s) 438, 480, 1108, 1375, 1666
BtsI GCAGTG 2 cut(s) 1054, 2182
BtsIMutI CAGTG 4 cut(s) 1054, 1982, 2136, 2182
Cac8I GCNNGC 6 cut(s) 525, 649, 969, 1225, 1408, 1575
CaiI CAGNNNCTG 2 cut(s) 68, 1825
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 3 cut(s) 437, 1176, 1780
Cfr10I RCCGGY 3 cut(s) 383, 503, 647
Cfr13I GGNCC 5 cut(s) 361, 716, 860, 1090, 1751
ClaI ATCGAT 1 cut(s) 2167
Csp6I GTAC 2 cut(s) 1663, 1962
CviAII CATG 9 cut(s) 876, 949, 1130, 1187, 1264, 1499, 1901, 2015, 2083
CviQI GTAC 2 cut(s) 1663, 1962
DinI GGCGCC 1 cut(s) 436
DpnI GATC 4 cut(s) 1890, 1896, 2166, 2170
DpnII GATC 4 cut(s) 1888, 1894, 2164, 2168
EaeI YGGCCR 3 cut(s) 505, 543, 645
EagI CGGCCG 1 cut(s) 505
Eam1104I CTCTTC 3 cut(s) 50, 299, 1034
EarI CTCTTC 3 cut(s) 50, 299, 1034
EciI GGCGGA 3 cut(s) 261, 301, 341
Ecl136II GAGCTC 1 cut(s) 1444
EclXI CGGCCG 1 cut(s) 505
Eco130I CCWWGG 3 cut(s) 150, 211, 787
Eco147I AGGCCT 1 cut(s) 2077
Eco24I GRGCYC 1 cut(s) 1446
Eco31I GGTCTC 1 cut(s) 349
Eco32I GATATC 1 cut(s) 36
Eco47I GGWCC 2 cut(s) 1090, 1751
Eco52I CGGCCG 1 cut(s) 505
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 1 cut(s) 1767
Eco88I CYCGRG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 754
EcoICRI GAGCTC 1 cut(s) 1444
EcoO109I RGGNCCY 2 cut(s) 860, 1751
EcoO65I GGTNACC 1 cut(s) 754
EcoRII CCWGG 4 cut(s) 642, 1218, 1756, 1843
EcoRV GATATC 1 cut(s) 36
EcoT14I CCWWGG 3 cut(s) 150, 211, 787
EcoT38I GRGCYC 1 cut(s) 1446
EgeI GGCGCC 1 cut(s) 436
EheI GGCGCC 1 cut(s) 436
ErhI CCWWGG 3 cut(s) 150, 211, 787
FaeI CATG 9 cut(s) 879, 952, 1133, 1190, 1267, 1502, 1904, 2018, 2086
FaqI GGGAC 2 cut(s) 1019, 1293
FatI CATG 9 cut(s) 875, 948, 1129, 1186, 1263, 1498, 1900, 2014, 2082
FauI CCCGC 2 cut(s) 522, 594
FbaI TGATCA 1 cut(s) 1888
FblI GTMKAC 1 cut(s) 1587
FokI GGATG 5 cut(s) 425, 467, 1095, 1382, 1653
FriOI GRGCYC 1 cut(s) 1446
FspBI CTAG 3 cut(s) 140, 2004, 2057
GlaI GCGC 3 cut(s) 436, 1175, 1779
GsaI CCCAGC 2 cut(s) 229, 1403
GsuI CTGGAG 3 cut(s) 455, 809, 1740
HaeII RGCGCY 1 cut(s) 438
HaeIII GGCC 7 cut(s) 363, 507, 545, 647, 718, 862, 2077
HapII CCGG 8 cut(s) 384, 504, 648, 801, 915, 930, 1030, 1068
HhaI GCGC 3 cut(s) 437, 1176, 1780
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 9 cut(s) 879, 952, 1133, 1190, 1267, 1502, 1904, 2018, 2086
Hin6I GCGC 3 cut(s) 435, 1174, 1778
HinP1I GCGC 3 cut(s) 435, 1174, 1778
HincII GTYRAC 3 cut(s) 466, 1348, 2011
HindII GTYRAC 3 cut(s) 466, 1348, 2011
HindIII AAGCTT 2 cut(s) 730, 1766
HpaI GTTAAC 1 cut(s) 2011
HpaII CCGG 8 cut(s) 384, 504, 648, 801, 915, 930, 1030, 1068
HphI GGTGA 5 cut(s) 493, 919, 1175, 1499, 1516
Hpy166II GTNNAC 7 cut(s) 55, 466, 1090, 1348, 1588, 2011, 2180
Hpy8I GTNNAC 7 cut(s) 55, 466, 1090, 1348, 1588, 2011, 2180
Hpy99I CGWCG 2 cut(s) 523, 2033
HpyCH4III ACNGT 4 cut(s) 1320, 1345, 1957, 1969
HpyCH4IV ACGT 3 cut(s) 219, 736, 2031
HpySE526I ACGT 3 cut(s) 219, 736, 2031
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 9 cut(s) 879, 952, 1133, 1190, 1267, 1502, 1904, 2018, 2086
HspAI GCGC 3 cut(s) 435, 1174, 1778
KasI GGCGCC 1 cut(s) 434
KroI GCCGGC 1 cut(s) 647
KroNI GCCGGC 1 cut(s) 649
Ksp22I TGATCA 1 cut(s) 1888
KspAI GTTAAC 1 cut(s) 2011
Kzo9I GATC 4 cut(s) 1888, 1894, 2164, 2168
LguI GCTCTTC 1 cut(s) 1034
LweI GCATC 4 cut(s) 608, 1117, 1217, 1360
MaeI CTAG 3 cut(s) 140, 2004, 2057
MaeII ACGT 3 cut(s) 219, 736, 2031
MaeIII GTNAC 4 cut(s) 220, 272, 754, 1163
MalI GATC 4 cut(s) 1890, 1896, 2166, 2170
MbiI CCGCTC 1 cut(s) 302
MboI GATC 4 cut(s) 1888, 1894, 2164, 2168
MfeI CAATTG 2 cut(s) 1245, 1797
MhlI GDGCHC 5 cut(s) 768, 939, 1446, 1645, 2182
MluCI AATT 8 cut(s) 686, 851, 898, 923, 979, 1072, 1245, 1797
Mly113I GGCGCC 1 cut(s) 435
MlyI GAGTC 5 cut(s) 86, 92, 469, 504, 627
MmeI TCCRAC 2 cut(s) 60, 444
MroNI GCCGGC 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 1429
MseI TTAA 3 cut(s) 1272, 1302, 2010
MslI CAYNNNNRTG 3 cut(s) 63, 932, 1671
MspA1I CMGCKG 4 cut(s) 265, 432, 1241, 1294
MspI CCGG 8 cut(s) 384, 504, 648, 801, 915, 930, 1030, 1068
MspR9I CCNGG 6 cut(s) 644, 1031, 1069, 1220, 1758, 1845
MunI CAATTG 2 cut(s) 1245, 1797
Mva1269I GAATGC 2 cut(s) 1995, 2193
MvaI CCWGG 4 cut(s) 644, 1220, 1758, 1845
MvnI CGCG 2 cut(s) 271, 589
NaeI GCCGGC 1 cut(s) 649
NarI GGCGCC 1 cut(s) 435
NciI CCSGG 2 cut(s) 1031, 1069
NdeII GATC 4 cut(s) 1888, 1894, 2164, 2168
NgoMIV GCCGGC 1 cut(s) 647
NlaIII CATG 9 cut(s) 879, 952, 1133, 1190, 1267, 1502, 1904, 2018, 2086
NlaIV GGNNCC 6 cut(s) 362, 436, 861, 1237, 1405, 1578
NmuCI GTSAC 2 cut(s) 272, 1163
NspI RCATGY 1 cut(s) 952
NspV TTCGAA 1 cut(s) 1037
OliI CACNNNNGTG 2 cut(s) 63, 932
PaeR7I CTCGAG 1 cut(s) 398
PagI TCATGA 1 cut(s) 1186
PceI AGGCCT 1 cut(s) 2077
PciI ACATGT 1 cut(s) 948
PciSI GCTCTTC 1 cut(s) 1034
PcsI WCGNNNNNNNCGW 1 cut(s) 536
PctI GAATGC 2 cut(s) 1995, 2193
PdiI GCCGGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 1429
PfeI GAWTC 6 cut(s) 403, 792, 804, 1331, 1472, 1676
PfoI TCCNGGA 2 cut(s) 1067, 1756
PleI GAGTC 5 cut(s) 85, 91, 469, 504, 626
PluTI GGCGCC 1 cut(s) 438
PpsI GAGTC 5 cut(s) 85, 91, 469, 504, 626
PpuMI RGGWCCY 1 cut(s) 1751
PscI ACATGT 1 cut(s) 948
PsiI TTATAA 1 cut(s) 1920
Psp124BI GAGCTC 1 cut(s) 1446
Psp1406I AACGTT 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 1751
Psp6I CCWGG 4 cut(s) 642, 1218, 1756, 1843
PspEI GGTNACC 1 cut(s) 754
PspFI CCCAGC 2 cut(s) 225, 1399
PspGI CCWGG 4 cut(s) 642, 1218, 1756, 1843
PspN4I GGNNCC 6 cut(s) 362, 436, 861, 1237, 1405, 1578
PspPI GGNCC 5 cut(s) 361, 716, 860, 1090, 1751
PspPPI RGGWCCY 1 cut(s) 1751
PsrI GAACNNNNNNTAC 2 cut(s) 1945, 1977
PstNI CAGNNNCTG 2 cut(s) 68, 1825
PvuII CAGCTG 1 cut(s) 1294
RsaI GTAC 2 cut(s) 1664, 1963
RsaNI GTAC 2 cut(s) 1663, 1962
RseI CAYNNNNRTG 3 cut(s) 63, 932, 1671
SacI GAGCTC 1 cut(s) 1446
SapI GCTCTTC 1 cut(s) 1034
SaqAI TTAA 3 cut(s) 1272, 1302, 2010
Sau3AI GATC 4 cut(s) 1888, 1894, 2164, 2168
Sau96I GGNCC 5 cut(s) 361, 716, 860, 1090, 1751
SchI GAGTC 5 cut(s) 86, 92, 469, 504, 627
ScrFI CCNGG 6 cut(s) 644, 1031, 1069, 1220, 1758, 1845
SduI GDGCHC 5 cut(s) 768, 939, 1446, 1645, 2182
SfaNI GCATC 4 cut(s) 608, 1117, 1217, 1360
SfcI CTRYAG 1 cut(s) 1965
SfoI GGCGCC 1 cut(s) 436
Sfr274I CTCGAG 1 cut(s) 398
SfuI TTCGAA 1 cut(s) 1037
SinI GGWCC 2 cut(s) 1090, 1751
SlaI CTCGAG 1 cut(s) 398
SmiMI CAYNNNNRTG 3 cut(s) 63, 932, 1671
SmlI CTYRAG 4 cut(s) 398, 1445, 1601, 1865
SmoI CTYRAG 4 cut(s) 398, 1445, 1601, 1865
Sse9I AATT 8 cut(s) 686, 851, 898, 923, 979, 1072, 1245, 1797
SseBI AGGCCT 1 cut(s) 2077
SspDI GGCGCC 1 cut(s) 434
SspI AATATT 1 cut(s) 1695
SspMI CTAG 3 cut(s) 140, 2004, 2057
SstI GAGCTC 1 cut(s) 1446
StuI AGGCCT 1 cut(s) 2077
StyD4I CCNGG 6 cut(s) 642, 1029, 1067, 1218, 1756, 1843
StyI CCWWGG 3 cut(s) 150, 211, 787
TaaI ACNGT 4 cut(s) 1320, 1345, 1957, 1969
TaiI ACGT 3 cut(s) 222, 739, 2034
TaqI TCGA 6 cut(s) 399, 486, 1037, 1423, 2152, 2167
TaqII GACCGA 1 cut(s) 525
TasI AATT 8 cut(s) 686, 851, 898, 923, 979, 1072, 1245, 1797
TauI GCSGC 4 cut(s) 246, 271, 435, 1241
TfiI GAWTC 6 cut(s) 403, 792, 804, 1331, 1472, 1676
Tru1I TTAA 3 cut(s) 1272, 1302, 2010
Tru9I TTAA 3 cut(s) 1272, 1302, 2010
TscAI CASTG 4 cut(s) 1054, 1989, 2143, 2182
TseFI GTSAC 2 cut(s) 272, 1163
Tsp45I GTSAC 2 cut(s) 272, 1163
TspDTI ATGAA 7 cut(s) 864, 1146, 1175, 1252, 1409, 1889, 2109
TspGWI ACGGA 2 cut(s) 40, 171
TspRI CASTG 4 cut(s) 1054, 1989, 2143, 2182
VneI GTGCAC 1 cut(s) 2178
VpaK11BI GGWCC 2 cut(s) 1090, 1751
XapI RAATTY 2 cut(s) 923, 979
XceI RCATGY 1 cut(s) 952
XcmI CCANNNNNNNNNTGG 2 cut(s) 748, 2096
XhoI CTCGAG 1 cut(s) 398
XmiI GTMKAC 1 cut(s) 1587
XmnI GAANNNNTTC 1 cut(s) 1429
XspI CTAG 3 cut(s) 140, 2004, 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.