MD12G1009300.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001328
Physical Location & Seq
Forward (+)
984189 .. 988577
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1009300.v1.1.491

Sequence Viewer

Length: 2358 bp
ATGGCAAAACTCTCTCTCTCTTTTCTGCCTTTGGTTCTTGCTATCATTCTATCTTCAGTTCGTCATTCCGAACAACGTCAATCCTCCGAAGCTCAGACCCTTTTGAGAATTACGCAACTCATAAACTTTCCCGTTGTGAAAAGCTTGAATAACGACACAGATTTTTGCAACTTTGAAGTGAATGCTGCTTTTACGGTCTCATGCTACGAAGGAAGCATAACCCAGCTGCATATAATCGGCGAAAAACCTGCTCCTTTGTTGCCTCAAAACTTTTCGATTGATTCCTTTGTAACAACTCTGGTTAAGCTTCCGAGGTTGAAGGTCCTCACATTGGTTTCTGTTGGTCTATGGGGAACCTTGCCGGCTAAAATCGCACGTTTATCATCACTCGAAATACTTAATGTGACTTCAAATTTCTTGAATGGCACCATCCCTCCAGAGCTTTCCTCTCTAACAAGCCTCCAGACTCTTGTACTCGATAACAACATGTTTTCTGGACCGCTTCCAGAAGGGCTGAGTTCACTTCCAGTTTTGTCTGTTTTGAGTTTGAAGAAAAACTCGTTCAATTCGTCCTTGCCGAGTTCATTGAGTGATTTGGGAAATCTTAGAGTTCTCGGGCTTTCTCAAAACCGTTTCCATGGGGAACTGCCGGATCTTAGTCGTTTGACAAACCTTCAAGTTCTTGAAGTGGGAGATAATGCTTTTGGACCTCAGTTTCCGAAACTTGGGAAAAAGTTGGTCACCCTTGTACTGAGAAAGAACAAGTTTAGGTCTTCCATCCCTGCTGAATTGAGCTCGTATTATCAGCTACAACGTCTAGACGTTTCTTCCAACGTGTTCATGGGGCCATTTCCTCCATCGCTGCTATCTCTACCTTCGATTACTTATCTTAATATTTCGGGAAACAAGTTTACAGGAATGCTTTCTGAAAATTTGCCTTGCAATGCTGAACTTAAAGCTGTGGATTTGTCCTCGAATCTTTTGACCGGAAGCTTACCTAAATGTCTTGAGTCGGATTCTAAGGACAGGGTTGTCCTGTATGCAAGGAATTGTTTAGCTACTAGTAATCAAAATCAACATCCGGTTCAATTTTGCCTCAATGGGGCGTTAGCTGTTGGAATCGTACCTGACCAAAGCAAGCGAAAGAAAGCCTCCAAAGCAGCCCTTGCCATAGGTATAATTGGAGGGATTTTCGGAGGTGTTCTACTTGTCGGTCTGATTTTCCTTGTACATAGAAGAATTAATACCAACAAGACATTGAAGAAATCTCCACCAAGATCAATAACAGAGAATGCATCAAGTGGATATACATCAAAGTTACTATCAGATGCAAGATATATATCTCAAACAATGAAGACGGGAGCGCTTGGCCTTCCAAGTTATCGAACCTTTTCACTCGAGGAACTTGAGGACGCAACACAAAACTTTGATACGTCTACTTTCATGGGTGAAGGCTCACATGGACAGATGTACAGAGGCCAGTTGAAGGATGGTTCTTTTGTTGCCATCAGATGCTTGAAAATGAAAGGAAGTCACAGCACTCAAAATTTCATGCATCACATAGAGCTGATTATGAAACTACGGCATCGCCATTTGGTCAGCGCACTTGGACACTGCTTTGAGTGTTACTTGGATGATTCGAGCGTCAGCAGAATATTTCTTGTTTTCGAATATGTACCATATGGCACACTCCGGAGCTGGATATCTGAGGGACATCCCAGACGATCACTCGCGTGGACACAACGCATAGCAGCTGCAATTGGCATAGCGAAGGGCATTCAGTTTTTGCACACAGGGATCATGCCTGGTGTATATTCAAGTAATCTTAAAATAACAGACATTTTACTGGATCAGAATTTTGGTGCGAAAATTAGCAGTTACAACCTTCCTCTGTTAGAAGAAAATATGGAACAGGGTGGTCATGGAGTCTCTTCAAGTGGATCCATAACTTCTAGTACCGGTGCAAGGAAGAAACACGACGATAGGACTGATGTTCATGACTTTGGAGTAATATTGCTGGAACTGATCAAGGGGAGACCGGTTAAGTCTGAGGCTCAAATCGAGGATTTAGAAGATCAGGTACAAGTGGCCCTTACAGCTGATGATGCAGCTCGAAGAAGCGTAGTTGATTCACGGATTAAGCAGACGTGCTTGGATCTGTCAGTGAAGACAATGATGGAAGTTTGTGTTCGGTGTCTGGGCCAGGATCCAGCAGATAGGCCTTCTATTGAGGATGTTCTGTGGAACTTGCAGTATGCTGAACAAGTTCAGGATGCATGGCAAGGAGGAGAATCCCAGAGCAGTGAGGACTCTCCGGTTTCATCTCCAAAACCTCCACGCCTCGCCTTCAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

786

Amino Acids

86.05

Weight (kDa)

8.73

Isoelectric Point (pI)

45.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 98 - 305 3.5e-12 Leucine-rich repeat region
Pkinase PF00069 479 - 747 3.3e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 480 - 748 6.2e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1031
Acc36I ACCTGC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 1436
AccII CGCG 1 cut(s) 1733
AccIII TCCGGA 1 cut(s) 1692
AciI CCGC 1 cut(s) 500
AclWI GGATC 8 cut(s) 660, 1805, 1857, 1935, 1948, 2163, 2201, 2214
AcsI RAATTY 4 cut(s) 412, 931, 1546, 1855
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 8 cut(s) 474, 750, 1125, 1230, 1472, 1677, 1957, 2082
AfeI AGCGCT 1 cut(s) 1365
AfiI CCNNNNNNNGG 5 cut(s) 331, 725, 1102, 1486, 1965
AflIII ACRYGT 2 cut(s) 486, 834
AgeI ACCGGT 2 cut(s) 1958, 2038
AhlI ACTAGT 1 cut(s) 1061
AjiI CACGTC 1 cut(s) 2148
AjnI CCWGG 2 cut(s) 1804, 2202
AleI CACNNNNGTG 1 cut(s) 1732
Alw21I GWGCWC 1 cut(s) 797
Alw26I GTCTC 3 cut(s) 202, 1933, 2029
AlwI GGATC 8 cut(s) 660, 1805, 1857, 1935, 1948, 2163, 2201, 2214
Ama87I CYCGRG 2 cut(s) 614, 1397
Aor13HI TCCGGA 1 cut(s) 1692
Aor51HI AGCGCT 1 cut(s) 1365
AoxI GGCC 6 cut(s) 845, 1369, 1477, 2088, 2200, 2219
ApeKI GCWGC 7 cut(s) 185, 226, 862, 1160, 1751, 1754, 2108
ApoI RAATTY 4 cut(s) 412, 931, 1546, 1855
AseI ATTAAT 1 cut(s) 1242
AsiGI ACCGGT 2 cut(s) 1958, 2038
Asp700I GAANNNNTTC 3 cut(s) 922, 1390, 2265
AspLEI GCGC 2 cut(s) 1366, 1604
AspS9I GGNCC 6 cut(s) 322, 497, 707, 845, 2089, 2200
AsuHPI GGTGA 2 cut(s) 733, 1460
AsuII TTCGAA 1 cut(s) 1668
AvaI CYCGRG 2 cut(s) 614, 1397
AvaII GGWCC 3 cut(s) 322, 497, 707
BamHI GGATCC 2 cut(s) 1940, 2206
BanI GGYRCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 797
BarI GAAGNNNNNNTAC 4 cut(s) 1228, 1260, 2064, 2096
BbsI GAAGAC 3 cut(s) 765, 1361, 2174
Bbv12I GWGCWC 1 cut(s) 797
BbvI GCAGC 7 cut(s) 172, 213, 849, 1172, 1741, 1763, 2120
BccI CCATC 6 cut(s) 437, 785, 865, 1484, 1514, 2170
BceAI ACGGC 1 cut(s) 1598
BcgI CGANNNNNNTGC 2 cut(s) 230, 264
BciT130I CCWGG 2 cut(s) 1806, 2204
BclI TGATCA 1 cut(s) 2025
BcoDI GTCTC 3 cut(s) 202, 1933, 2029
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 3 cut(s) 818, 1062, 1953
BfoI RGCGCY 1 cut(s) 1367
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 7 cut(s) 186, 227, 863, 1161, 1752, 1755, 2109
BlsI GCNGC 7 cut(s) 187, 228, 864, 1162, 1753, 1756, 2110
Bme1390I CCNGG 2 cut(s) 1806, 2204
Bme18I GGWCC 3 cut(s) 322, 497, 707
BmeT110I CYCGRG 2 cut(s) 614, 1397
BmgBI CACGTC 1 cut(s) 2148
BmgT120I GGNCC 6 cut(s) 322, 497, 707, 845, 2089, 2200
BmiI GGNNCC 5 cut(s) 355, 427, 846, 1942, 2208
BmrFI CCNGG 2 cut(s) 1806, 2204
BmsI GCATC 7 cut(s) 1304, 1318, 1502, 1564, 1594, 2095, 2263
BpiI GAAGAC 3 cut(s) 765, 1361, 2174
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 2 cut(s) 420, 446
Bpu14I TTCGAA 1 cut(s) 1668
BpuEI CTTGAG 2 cut(s) 1028, 1427
BsaBI GATNNNNATC 2 cut(s) 1309, 1339
BsaI GGTCTC 2 cut(s) 202, 2029
BsaJI CCNNGG 2 cut(s) 311, 637
BsaWI WCCGGW 6 cut(s) 986, 1081, 1692, 1958, 2038, 2314
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 5 cut(s) 331, 725, 1102, 1486, 1965
Bse118I RCCGGY 3 cut(s) 361, 1958, 2038
Bse1I ACTGG 3 cut(s) 527, 1480, 1851
Bse3DI GCAATG 1 cut(s) 949
Bse8I GATNNNNATC 2 cut(s) 1309, 1339
BseAI TCCGGA 1 cut(s) 1692
BseBI CCWGG 2 cut(s) 1806, 2204
BseDI CCNNGG 2 cut(s) 311, 637
BseGI GGATG 8 cut(s) 429, 777, 1078, 1495, 1639, 1714, 2239, 2278
BseJI GATNNNNATC 2 cut(s) 1309, 1339
BseLI CCNNNNNNNGG 5 cut(s) 331, 725, 1102, 1486, 1965
BseMI GCAATG 1 cut(s) 949
BseMII CTCAG 6 cut(s) 107, 506, 725, 743, 1698, 2040
BseNI ACTGG 3 cut(s) 527, 1480, 1851
BseRI GAGGAG 1 cut(s) 2301
BseXI GCAGC 7 cut(s) 172, 213, 849, 1172, 1741, 1763, 2120
BseYI CCCAGC 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 1733
BshFI GGCC 6 cut(s) 847, 1371, 1479, 2090, 2202, 2221
BshNI GGYRCC 1 cut(s) 425
BshTI ACCGGT 2 cut(s) 1958, 2038
BsiHKAI GWGCWC 1 cut(s) 797
BsiHKCI CYCGRG 2 cut(s) 614, 1397
BsiSI CCGG 8 cut(s) 362, 650, 987, 1082, 1693, 1959, 2039, 2315
BslFI GGGAC 1 cut(s) 1725
BslI CCNNNNNNNGG 5 cut(s) 331, 725, 1102, 1486, 1965
BsmAI GTCTC 3 cut(s) 202, 1933, 2029
BsmFI GGGAC 1 cut(s) 1725
BsmI GAATGC 4 cut(s) 187, 924, 1297, 1776
BsnI GGCC 6 cut(s) 847, 1371, 1479, 2090, 2202, 2221
Bso31I GGTCTC 2 cut(s) 202, 2029
BsoBI CYCGRG 2 cut(s) 614, 1397
Bsp119I TTCGAA 1 cut(s) 1668
Bsp1286I GDGCHC 1 cut(s) 797
Bsp13I TCCGGA 1 cut(s) 1692
Bsp1407I TGTACA 2 cut(s) 1228, 1470
Bsp19I CCATGG 1 cut(s) 637
BspACI CCGC 1 cut(s) 500
BspANI GGCC 6 cut(s) 847, 1371, 1479, 2090, 2202, 2221
BspCNI CTCAG 6 cut(s) 106, 507, 724, 744, 1699, 2041
BspEI TCCGGA 1 cut(s) 1692
BspFNI CGCG 1 cut(s) 1733
BspHI TCATGA 1 cut(s) 1996
BspLI GGNNCC 5 cut(s) 355, 427, 846, 1942, 2208
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 8 cut(s) 660, 1805, 1857, 1935, 1948, 2163, 2201, 2214
BspT104I TTCGAA 1 cut(s) 1668
BspT107I GGYRCC 1 cut(s) 425
BspTNI GGTCTC 2 cut(s) 202, 2029
BsrDI GCAATG 1 cut(s) 949
BsrFI RCCGGY 3 cut(s) 361, 1958, 2038
BsrGI TGTACA 2 cut(s) 1228, 1470
BsrI ACTGG 3 cut(s) 527, 1480, 1851
BssAI RCCGGY 3 cut(s) 361, 1958, 2038
BssECI CCNNGG 2 cut(s) 311, 637
BssT1I CCWWGG 1 cut(s) 637
Bst2UI CCWGG 2 cut(s) 1806, 2204
Bst4CI ACNGT 2 cut(s) 196, 632
Bst6I CTCTTC 1 cut(s) 1936
BstAPI GCANNNNNTGC 1 cut(s) 1166
BstAUI TGTACA 2 cut(s) 1228, 1470
BstBI TTCGAA 1 cut(s) 1668
BstC8I GCNNGC 2 cut(s) 363, 1139
BstDEI CTNAG 9 cut(s) 93, 515, 605, 656, 711, 752, 1020, 1707, 2049
BstDSI CCRYGG 1 cut(s) 637
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 8 cut(s) 429, 777, 1078, 1495, 1639, 1714, 2239, 2278
BstFNI CGCG 1 cut(s) 1733
BstH2I RGCGCY 1 cut(s) 1367
BstHHI GCGC 2 cut(s) 1366, 1604
BstMAI GTCTC 3 cut(s) 202, 1933, 2029
BstMWI GCNNNNNNNGC 5 cut(s) 371, 1157, 1166, 2096, 2105
BstNI CCWGG 2 cut(s) 1806, 2204
BstNSI RCATGY 1 cut(s) 490
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 2 cut(s) 1804, 2202
BstUI CGCG 1 cut(s) 1733
BstV1I GCAGC 7 cut(s) 172, 213, 849, 1172, 1741, 1763, 2120
BstV2I GAAGAC 3 cut(s) 765, 1361, 2174
BstX2I RGATCY 4 cut(s) 652, 1940, 2155, 2206
BstYI RGATCY 4 cut(s) 652, 1940, 2155, 2206
BsuRI GGCC 6 cut(s) 847, 1371, 1479, 2090, 2202, 2221
BtgI CCRYGG 1 cut(s) 637
BtgZI GCGATG 2 cut(s) 843, 1571
BtrI CACGTC 1 cut(s) 2148
BtsCI GGATG 8 cut(s) 429, 777, 1078, 1495, 1639, 1714, 2239, 2278
BtsI GCAGTG 2 cut(s) 1612, 2308
BtsIMutI CAGTG 3 cut(s) 1612, 2169, 2308
BveI ACCTGC 1 cut(s) 256
Cac8I GCNNGC 2 cut(s) 363, 1139
CciI TCATGA 1 cut(s) 1996
CfoI GCGC 2 cut(s) 1366, 1604
Cfr10I RCCGGY 3 cut(s) 361, 1958, 2038
Cfr13I GGNCC 6 cut(s) 322, 497, 707, 845, 2089, 2200
CseI GACGC 2 cut(s) 1421, 1633
Csp6I GTAC 8 cut(s) 473, 749, 1124, 1229, 1471, 1676, 1956, 2081
CspAI ACCGGT 2 cut(s) 1958, 2038
CviQI GTAC 8 cut(s) 473, 749, 1124, 1229, 1471, 1676, 1956, 2081
DdeI CTNAG 9 cut(s) 93, 515, 605, 656, 711, 752, 1020, 1707, 2049
DrdI GACNNNNNNGTC 1 cut(s) 1031
DseDI GACNNNNNNGTC 1 cut(s) 1031
Eam1104I CTCTTC 1 cut(s) 1936
EarI CTCTTC 1 cut(s) 1936
Ecl136II GAGCTC 1 cut(s) 795
Eco130I CCWWGG 1 cut(s) 637
Eco147I AGGCCT 1 cut(s) 2221
Eco24I GRGCYC 1 cut(s) 797
Eco31I GGTCTC 2 cut(s) 202, 2029
Eco32I GATATC 1 cut(s) 1704
Eco47I GGWCC 3 cut(s) 322, 497, 707
Eco47III AGCGCT 1 cut(s) 1365
Eco53kI GAGCTC 1 cut(s) 795
Eco57I CTGAAG 1 cut(s) 39
Eco88I CYCGRG 2 cut(s) 614, 1397
Eco91I GGTNACC 1 cut(s) 739
EcoICRI GAGCTC 1 cut(s) 795
EcoO109I RGGNCCY 1 cut(s) 322
EcoO65I GGTNACC 1 cut(s) 739
EcoRII CCWGG 2 cut(s) 1804, 2202
EcoRV GATATC 1 cut(s) 1704
EcoT14I CCWWGG 1 cut(s) 637
EcoT22I ATGCAT 3 cut(s) 1297, 1557, 2278
EcoT38I GRGCYC 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 637
FalI AAGNNNNNCTT 4 cut(s) 1149, 1181, 2076, 2108
FaqI GGGAC 1 cut(s) 1725
FauNDI CATATG 1 cut(s) 1681
FbaI TGATCA 1 cut(s) 2025
FblI GTMKAC 1 cut(s) 1436
Fnu4HI GCNGC 7 cut(s) 186, 227, 863, 1161, 1752, 1755, 2109
FokI GGATG 8 cut(s) 416, 764, 1065, 1502, 1646, 1701, 2246, 2285
FriOI GRGCYC 1 cut(s) 797
Fsp4HI GCNGC 7 cut(s) 186, 227, 863, 1161, 1752, 1755, 2109
FspBI CTAG 3 cut(s) 818, 1062, 1953
GlaI GCGC 2 cut(s) 1365, 1603
GluI GCNGC 7 cut(s) 186, 227, 863, 1161, 1752, 1755, 2109
GsaI CCCAGC 1 cut(s) 226
GsuI CTGGAG 2 cut(s) 420, 446
HaeII RGCGCY 1 cut(s) 1367
HaeIII GGCC 6 cut(s) 847, 1371, 1479, 2090, 2202, 2221
HapII CCGG 8 cut(s) 362, 650, 987, 1082, 1693, 1959, 2039, 2315
HgaI GACGC 2 cut(s) 1421, 1633
HhaI GCGC 2 cut(s) 1366, 1604
Hin6I GCGC 2 cut(s) 1364, 1602
HinP1I GCGC 2 cut(s) 1364, 1602
HindIII AAGCTT 3 cut(s) 142, 305, 991
HpaII CCGG 8 cut(s) 362, 650, 987, 1082, 1693, 1959, 2039, 2315
HphI GGTGA 2 cut(s) 733, 1460
Hpy166II GTNNAC 4 cut(s) 521, 912, 1437, 1737
Hpy8I GTNNAC 4 cut(s) 521, 912, 1437, 1737
Hpy99I CGWCG 1 cut(s) 1982
HpyCH4III ACNGT 2 cut(s) 196, 632
HpyCH4IV ACGT 7 cut(s) 76, 376, 814, 822, 834, 1433, 2147
HpyF10VI GCNNNNNNNGC 5 cut(s) 371, 1157, 1166, 2096, 2105
HpyF3I CTNAG 9 cut(s) 93, 515, 605, 656, 711, 752, 1020, 1707, 2049
HpySE526I ACGT 7 cut(s) 76, 376, 814, 822, 834, 1433, 2147
HspAI GCGC 2 cut(s) 1364, 1602
Kpn2I TCCGGA 1 cut(s) 1692
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Ksp22I TGATCA 1 cut(s) 2025
LmnI GCTCC 3 cut(s) 256, 1361, 1695
Lsp1109I GCAGC 7 cut(s) 172, 213, 849, 1172, 1741, 1763, 2120
LweI GCATC 7 cut(s) 1304, 1318, 1502, 1564, 1594, 2095, 2263
MaeI CTAG 3 cut(s) 818, 1062, 1953
MaeII ACGT 7 cut(s) 76, 376, 814, 822, 834, 1433, 2147
MaeIII GTNAC 7 cut(s) 289, 403, 739, 1317, 1532, 1625, 1877
MfeI CAATTG 1 cut(s) 1758
MflI RGATCY 4 cut(s) 652, 1940, 2155, 2206
MhlI GDGCHC 1 cut(s) 797
MlyI GAGTC 4 cut(s) 460, 1019, 1935, 2303
MmeI TCCRAC 3 cut(s) 855, 993, 1096
Mph1103I ATGCAT 3 cut(s) 1297, 1557, 2278
MroI TCCGGA 1 cut(s) 1692
MroNI GCCGGC 1 cut(s) 361
MroXI GAANNNNTTC 3 cut(s) 922, 1390, 2265
MseI TTAA 8 cut(s) 303, 399, 891, 954, 1242, 1827, 2043, 2139
MslI CAYNNNNRTG 1 cut(s) 1732
MspA1I CMGCKG 3 cut(s) 226, 1754, 2099
MspI CCGG 8 cut(s) 362, 650, 987, 1082, 1693, 1959, 2039, 2315
MspR9I CCNGG 2 cut(s) 1806, 2204
MunI CAATTG 1 cut(s) 1758
Mva1269I GAATGC 4 cut(s) 187, 924, 1297, 1776
MvaI CCWGG 2 cut(s) 1806, 2204
MvnI CGCG 1 cut(s) 1733
MwoI GCNNNNNNNGC 5 cut(s) 371, 1157, 1166, 2096, 2105
NaeI GCCGGC 1 cut(s) 363
NcoI CCATGG 1 cut(s) 637
NdeI CATATG 1 cut(s) 1681
NgoMIV GCCGGC 1 cut(s) 361
NlaIV GGNNCC 5 cut(s) 355, 427, 846, 1942, 2208
NmeAIII GCCGAG 1 cut(s) 603
NmuCI GTSAC 3 cut(s) 403, 739, 1532
NsiI ATGCAT 3 cut(s) 1297, 1557, 2278
NspI RCATGY 1 cut(s) 490
NspV TTCGAA 1 cut(s) 1668
OliI CACNNNNGTG 1 cut(s) 1732
PaeR7I CTCGAG 1 cut(s) 1397
PagI TCATGA 1 cut(s) 1996
PceI AGGCCT 1 cut(s) 2221
PciI ACATGT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 2 cut(s) 566, 575
PctI GAATGC 4 cut(s) 187, 924, 1297, 1776
PdiI GCCGGC 1 cut(s) 363
PdmI GAANNNNTTC 3 cut(s) 922, 1390, 2265
PfeI GAWTC 7 cut(s) 281, 976, 1016, 1119, 1637, 2129, 2291
PinAI ACCGGT 2 cut(s) 1958, 2038
PkrI GCNGC 7 cut(s) 187, 228, 864, 1162, 1753, 1756, 2110
PleI GAGTC 4 cut(s) 460, 1018, 1934, 2303
PpsI GAGTC 4 cut(s) 460, 1018, 1934, 2303
PpuMI RGGWCCY 1 cut(s) 322
PscI ACATGT 1 cut(s) 486
PshBI ATTAAT 1 cut(s) 1242
Psp124BI GAGCTC 1 cut(s) 797
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 2 cut(s) 1804, 2202
PspEI GGTNACC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 222
PspGI CCWGG 2 cut(s) 1804, 2202
PspN4I GGNNCC 5 cut(s) 355, 427, 846, 1942, 2208
PspPI GGNCC 6 cut(s) 322, 497, 707, 845, 2089, 2200
PspPPI RGGWCCY 1 cut(s) 322
PspXI VCTCGAGB 1 cut(s) 1397
PsuI RGATCY 4 cut(s) 652, 1940, 2155, 2206
PvuII CAGCTG 3 cut(s) 226, 1754, 2099
RsaI GTAC 8 cut(s) 474, 750, 1125, 1230, 1472, 1677, 1957, 2082
RsaNI GTAC 8 cut(s) 473, 749, 1124, 1229, 1471, 1676, 1956, 2081
RseI CAYNNNNRTG 1 cut(s) 1732
SacI GAGCTC 1 cut(s) 797
SaqAI TTAA 8 cut(s) 303, 399, 891, 954, 1242, 1827, 2043, 2139
SatI GCNGC 7 cut(s) 186, 227, 863, 1161, 1752, 1755, 2109
Sau96I GGNCC 6 cut(s) 322, 497, 707, 845, 2089, 2200
SchI GAGTC 4 cut(s) 460, 1019, 1935, 2303
ScrFI CCNGG 2 cut(s) 1806, 2204
SduI GDGCHC 1 cut(s) 797
SfaNI GCATC 7 cut(s) 1304, 1318, 1502, 1564, 1594, 2095, 2263
Sfr274I CTCGAG 1 cut(s) 1397
SfuI TTCGAA 1 cut(s) 1668
SinI GGWCC 3 cut(s) 322, 497, 707
SlaI CTCGAG 1 cut(s) 1397
SmiMI CAYNNNNRTG 1 cut(s) 1732
SmlI CTYRAG 3 cut(s) 1007, 1397, 1406
SmoI CTYRAG 3 cut(s) 1007, 1397, 1406
SpeI ACTAGT 1 cut(s) 1061
SseBI AGGCCT 1 cut(s) 2221
SsiI CCGC 1 cut(s) 500
SspI AATATT 3 cut(s) 895, 1656, 2013
SspMI CTAG 3 cut(s) 818, 1062, 1953
SstI GAGCTC 1 cut(s) 797
StuI AGGCCT 1 cut(s) 2221
StyD4I CCNGG 2 cut(s) 1804, 2202
StyI CCWWGG 1 cut(s) 637
TaaI ACNGT 2 cut(s) 196, 632
TaiI ACGT 7 cut(s) 79, 379, 817, 825, 837, 1436, 2150
TaqII GACCGA 1 cut(s) 1202
TatI WGTACW 4 cut(s) 472, 748, 1228, 1470
TfiI GAWTC 7 cut(s) 281, 976, 1016, 1119, 1637, 2129, 2291
Tru1I TTAA 8 cut(s) 303, 399, 891, 954, 1242, 1827, 2043, 2139
Tru9I TTAA 8 cut(s) 303, 399, 891, 954, 1242, 1827, 2043, 2139
TscAI CASTG 3 cut(s) 1619, 2169, 2308
TseFI GTSAC 3 cut(s) 403, 739, 1532
TseI GCWGC 7 cut(s) 185, 226, 862, 1160, 1751, 1754, 2108
Tsp45I GTSAC 3 cut(s) 403, 739, 1532
TspDTI ATGAA 9 cut(s) 573, 829, 1367, 1432, 1538, 1540, 1589, 1985, 2310
TspGWI ACGGA 1 cut(s) 2149
TspRI CASTG 3 cut(s) 1619, 2169, 2308
VpaK11BI GGWCC 3 cut(s) 322, 497, 707
VspI ATTAAT 1 cut(s) 1242
XapI RAATTY 4 cut(s) 412, 931, 1546, 1855
XbaI TCTAGA 1 cut(s) 817
XceI RCATGY 1 cut(s) 490
XcmI CCANNNNNNNNNTGG 2 cut(s) 838, 1487
XhoI CTCGAG 1 cut(s) 1397
XmiI GTMKAC 1 cut(s) 1436
XmnI GAANNNNTTC 3 cut(s) 922, 1390, 2265
XspI CTAG 3 cut(s) 818, 1062, 1953
Zsp2I ATGCAT 3 cut(s) 1297, 1557, 2278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.