MD03G1011000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012243
Physical Location & Seq
Forward (+)
824166 .. 824773
608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1011000.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGGCTCTCAGTTCCGGCCTTATGTAAAAGGCCCATACTTTTTCTCACAACTCCGCCATTCACCAAATAAAAAGGCGCCGTCATCGCATTCAGCGGAGGAGAAAAATTTCAGCGAGAGAGAAGAAACAATGAAGGGGTCGGACTCTGAAACCTCAAGGAAATCCGGCAACAACAAAGACAAGTCCACAAAGCCTCCCTTCATTCCAGCCAAAGACGACACCAAACCAGTTCTCCAAGACCCTATACTGAGATCCGACCCGATTGAGACGGAGGAAGCTGTGCTCCGGTTGCCTCCCTTCCCTATTGCCAGATCAACAAACCCTCCGCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.48

Weight (kDa)

9.43

Isoelectric Point (pI)

78.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 3 cut(s) 55, 95, 326
AclWI GGATC 1 cut(s) 246
AcsI RAATTY 1 cut(s) 106
AcyI GRCGYC 1 cut(s) 77
AluBI AGCT 1 cut(s) 278
AluI AGCT 1 cut(s) 278
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 1 cut(s) 246
AoxI GGCC 2 cut(s) 17, 31
ApoI RAATTY 1 cut(s) 106
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 54
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 285
BceAI ACGGC 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 1 cut(s) 78
BpuEI CTTGAG 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 77
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 5 cut(s) 178, 208, 216, 246, 307
Bse1I ACTGG 1 cut(s) 227
BseMII CTCAG 2 cut(s) 23, 239
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 113
BshFI GGCC 2 cut(s) 19, 33
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 285
BsiSI CCGG 3 cut(s) 16, 165, 286
BsmAI GTCTC 1 cut(s) 260
BsmBI CGTCTC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 2 cut(s) 19, 33
Bsp1286I GDGCHC 2 cut(s) 8, 285
Bsp143I GATC 2 cut(s) 251, 311
BspACI CCGC 3 cut(s) 55, 95, 326
BspANI GGCC 2 cut(s) 19, 33
BspCNI CTCAG 2 cut(s) 22, 240
BspLI GGNNCC 1 cut(s) 78
BspPI GGATC 1 cut(s) 246
BspT107I GGYRCC 1 cut(s) 76
BsrI ACTGG 1 cut(s) 227
BssMI GATC 2 cut(s) 251, 311
BssNI GRCGYC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 77
BstDEI CTNAG 2 cut(s) 9, 248
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 2 cut(s) 254, 314
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 2 cut(s) 251, 311
BstMWI GCNNNNNNNGC 2 cut(s) 85, 289
BstX2I RGATCY 1 cut(s) 251
BstYI RGATCY 1 cut(s) 251
BsuRI GGCC 2 cut(s) 19, 33
BtgZI GCGATG 1 cut(s) 69
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 32
CviJI RGCY 6 cut(s) 6, 19, 33, 193, 209, 278
CviKI_1 RGCY 6 cut(s) 6, 19, 33, 193, 209, 278
DdeI CTNAG 2 cut(s) 9, 248
DinI GGCGCC 1 cut(s) 78
DpnI GATC 2 cut(s) 253, 313
DpnII GATC 2 cut(s) 251, 311
EciI GGCGGA 2 cut(s) 44, 315
Eco24I GRGCYC 1 cut(s) 8
EcoT38I GRGCYC 1 cut(s) 8
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
Esp3I CGTCTC 1 cut(s) 260
FaiI YATR 3 cut(s) 24, 37, 245
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FriOI GRGCYC 1 cut(s) 8
GlaI GCGC 1 cut(s) 78
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 2 cut(s) 19, 33
HapII CCGG 3 cut(s) 16, 165, 286
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 77
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 1 cut(s) 143
HpaII CCGG 3 cut(s) 16, 165, 286
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 1 cut(s) 186
Hpy188I TCNGA 3 cut(s) 142, 148, 256
Hpy8I GTNNAC 1 cut(s) 186
HpyAV CCTTC 3 cut(s) 127, 208, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 289
HpyF3I CTNAG 2 cut(s) 9, 248
Hsp92I GRCGYC 1 cut(s) 77
HspAI GCGC 1 cut(s) 77
KasI GGCGCC 1 cut(s) 76
Kzo9I GATC 2 cut(s) 251, 311
LmnI GCTCC 1 cut(s) 288
LpnPI CCDG 6 cut(s) 29, 178, 219, 240, 299, 322
MalI GATC 2 cut(s) 253, 313
MboI GATC 2 cut(s) 251, 311
MboII GAAGA 1 cut(s) 134
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 2 cut(s) 8, 285
MluCI AATT 1 cut(s) 106
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 1 cut(s) 137
MmeI TCCRAC 2 cut(s) 120, 279
MnlI CCTC 6 cut(s) 91, 163, 204, 265, 303, 333
MroXI GAANNNNTTC 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 3 cut(s) 16, 165, 286
Mva1269I GAATGC 1 cut(s) 88
MwoI GCNNNNNNNGC 2 cut(s) 85, 289
NarI GGCGCC 1 cut(s) 77
NdeII GATC 2 cut(s) 251, 311
NlaIV GGNNCC 1 cut(s) 78
PctI GAATGC 1 cut(s) 88
PdmI GAANNNNTTC 1 cut(s) 107
PleI GAGTC 1 cut(s) 137
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 1 cut(s) 32
PsuI RGATCY 1 cut(s) 251
Sau3AI GATC 2 cut(s) 251, 311
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 1 cut(s) 137
SduI GDGCHC 2 cut(s) 8, 285
SetI ASST 2 cut(s) 155, 280
SfoI GGCGCC 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
Sse9I AATT 1 cut(s) 106
SsiI CCGC 3 cut(s) 55, 95, 326
SspDI GGCGCC 1 cut(s) 76
TasI AATT 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 146, 190
TspGWI ACGGA 1 cut(s) 284
XapI RAATTY 1 cut(s) 106
XmnI GAANNNNTTC 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.