Rmu_sc0005344.1_g000012

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005344.1
OG0012243
Physical Location & Seq
Reverse (-)
52341 .. 55316
2976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005344.1_g000012.1.cds

Sequence Viewer

Length: 669 bp
atggaggccagtaacgaagagaagaagcccacaaagcctccatccgttctgggtaaagatgacagcaaacctgtgcttcaagacccaattctgacgtcggacccaattgagacagaggaaggcgtgctgagattgcctccatcttccctactccctgaccatcaagaaacgcttaacactaactcttcttttactatattcacgaagccaacgctgtgcattctaattgtcagcgatgatatgagcctcagtgaattaatgatcgaagccctgggtgaatgtgaagtaagggaccagcaagatgctgattttgtgttgctgcatcagttgacaaacttttcagacattgtgacagctctgaaaaaccagttcgatataggttcacctactgatgtcctacttcttgacttggatactttgtacaaggaattggatgagatggagaagataaggaagagtcaagatgaggaaaagatgttttatgctgcatttgaatatgtgtactccgactgtgtgaaaaaccaagagagtgtgaaaagcagggaggacagggaagtagcgcataaatacatgcctatttctcatttcattgttggtattttgagctatgagatatccgatacagacaaagtcaacaagaaattcagagaggagaagttcaagatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.41

Weight (kDa)

4.56

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 98
AcsI RAATTY 1 cut(s) 641
AcyI GRCGYC 1 cut(s) 95
AfaI GTAC 2 cut(s) 422, 503
AgsI TTSAA 3 cut(s) 80, 494, 661
AjnI CCWGG 1 cut(s) 270
AluBI AGCT 2 cut(s) 356, 606
AluI AGCT 2 cut(s) 356, 606
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 319, 485
ApoI RAATTY 1 cut(s) 641
AseI ATTAAT 1 cut(s) 257
AspLEI GCGC 1 cut(s) 562
AspS9I GGNCC 2 cut(s) 100, 292
AsuHPI GGTGA 2 cut(s) 287, 375
AvaII GGWCC 2 cut(s) 100, 292
BbvI GCAGC 2 cut(s) 306, 472
BccI CCATC 4 cut(s) 49, 148, 168, 433
BciT130I CCWGG 1 cut(s) 272
BciVI GTATCC 1 cut(s) 406
BcoDI GTCTC 1 cut(s) 104
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 2 cut(s) 320, 486
BlsI GCNGC 2 cut(s) 321, 487
Bme1390I CCNGG 1 cut(s) 272
Bme18I GGWCC 2 cut(s) 100, 292
BmgT120I GGNCC 2 cut(s) 100, 292
BmiI GGNNCC 2 cut(s) 102, 293
BmrFI CCNGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 292, 331
BplI GAGNNNNNCTC 2 cut(s) 121, 153
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 2 cut(s) 270, 271
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bse1I ACTGG 2 cut(s) 9, 367
BseBI CCWGG 1 cut(s) 272
BseDI CCNNGG 2 cut(s) 270, 271
BseGI GGATG 2 cut(s) 41, 439
BseMII CTCAG 2 cut(s) 119, 262
BseNI ACTGG 2 cut(s) 9, 367
BseRI GAGGAG 1 cut(s) 665
BseXI GCAGC 2 cut(s) 306, 472
BshFI GGCC 1 cut(s) 8
BslFI GGGAC 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 104
BsmFI GGGAC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 1 cut(s) 8
Bsp1407I TGTACA 1 cut(s) 420
Bsp143I GATC 1 cut(s) 261
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 120, 261
BspLI GGNNCC 2 cut(s) 102, 293
BsrGI TGTACA 1 cut(s) 420
BsrI ACTGG 2 cut(s) 9, 367
BssECI CCNNGG 2 cut(s) 270, 271
BssMI GATC 1 cut(s) 261
BssNI GRCGYC 1 cut(s) 95
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 1 cut(s) 512
Bst6I CTCTTC 3 cut(s) 12, 190, 449
BstACI GRCGYC 1 cut(s) 95
BstAUI TGTACA 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 2 cut(s) 128, 248
BstF5I GGATG 2 cut(s) 41, 439
BstHHI GCGC 1 cut(s) 562
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 2 cut(s) 34, 133
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 270
BstV1I GCAGC 2 cut(s) 306, 472
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 1 cut(s) 8
BtgZI GCGATG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 41, 439
BtsIMutI CAGTG 1 cut(s) 256
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 1 cut(s) 562
Cfr13I GGNCC 2 cut(s) 100, 292
Csp6I GTAC 2 cut(s) 421, 502
CviAII CATG 1 cut(s) 571
CviJI RGCY 8 cut(s) 8, 28, 37, 208, 246, 269, 356, 606
CviKI_1 RGCY 8 cut(s) 8, 28, 37, 208, 246, 269, 356, 606
CviQI GTAC 2 cut(s) 421, 502
DdeI CTNAG 2 cut(s) 128, 248
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
Eam1104I CTCTTC 3 cut(s) 12, 190, 449
EarI CTCTTC 3 cut(s) 12, 190, 449
Eco32I GATATC 1 cut(s) 615
Eco47I GGWCC 2 cut(s) 100, 292
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 615
FaeI CATG 1 cut(s) 574
FaiI YATR 8 cut(s) 197, 242, 377, 483, 498, 564, 572, 609
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 1 cut(s) 305
FatI CATG 1 cut(s) 570
Fnu4HI GCNGC 2 cut(s) 320, 486
FokI GGATG 2 cut(s) 28, 446
Fsp4HI GCNGC 2 cut(s) 320, 486
GlaI GCGC 1 cut(s) 561
GluI GCNGC 2 cut(s) 320, 486
HaeIII GGCC 1 cut(s) 8
HhaI GCGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 1 cut(s) 574
Hin6I GCGC 1 cut(s) 560
HinP1I GCGC 1 cut(s) 560
HincII GTYRAC 2 cut(s) 330, 634
HindII GTYRAC 2 cut(s) 330, 634
HinfI GANTC 1 cut(s) 457
HphI GGTGA 2 cut(s) 287, 375
Hpy166II GTNNAC 4 cut(s) 330, 383, 502, 634
Hpy188I TCNGA 7 cut(s) 93, 100, 343, 360, 508, 619, 647
Hpy188III TCNNGA 6 cut(s) 80, 164, 202, 404, 461, 661
Hpy8I GTNNAC 4 cut(s) 330, 383, 502, 634
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 113
HpyCH4III ACNGT 1 cut(s) 512
HpyCH4IV ACGT 1 cut(s) 95
HpyCH4V TGCA 3 cut(s) 219, 322, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 133
HpyF3I CTNAG 2 cut(s) 128, 248
HpySE526I ACGT 1 cut(s) 95
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 1 cut(s) 574
HspAI GCGC 1 cut(s) 560
Kzo9I GATC 1 cut(s) 261
Lsp1109I GCAGC 2 cut(s) 306, 472
LweI GCATC 2 cut(s) 292, 331
MaeII ACGT 1 cut(s) 95
MaeIII GTNAC 2 cut(s) 11, 349
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 6 cut(s) 29, 34, 135, 177, 457, 466
MfeI CAATTG 1 cut(s) 105
MluCI AATT 6 cut(s) 87, 105, 225, 254, 428, 641
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 2 cut(s) 78, 531
MnlI CCTC 7 cut(s) 48, 109, 147, 257, 460, 538, 643
MseI TTAA 2 cut(s) 174, 257
MspR9I CCNGG 1 cut(s) 272
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 272
MwoI GCNNNNNNNGC 2 cut(s) 34, 133
NdeII GATC 1 cut(s) 261
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 2 cut(s) 102, 293
NmuCI GTSAC 1 cut(s) 349
NspI RCATGY 1 cut(s) 574
PasI CCCWGGG 1 cut(s) 271
PctI GAATGC 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 629
PkrI GCNGC 2 cut(s) 321, 487
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PshBI ATTAAT 1 cut(s) 257
Psp6I CCWGG 1 cut(s) 270
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 2 cut(s) 102, 293
PspPI GGNCC 2 cut(s) 100, 292
PsyI GACNNNGTC 1 cut(s) 629
RsaI GTAC 2 cut(s) 422, 503
RsaNI GTAC 2 cut(s) 421, 502
SaqAI TTAA 2 cut(s) 174, 257
SatI GCNGC 2 cut(s) 320, 486
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 2 cut(s) 100, 292
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 1 cut(s) 272
SetI ASST 6 cut(s) 73, 98, 358, 382, 388, 608
SfaNI GCATC 2 cut(s) 292, 331
SinI GGWCC 2 cut(s) 100, 292
Sse9I AATT 6 cut(s) 87, 105, 225, 254, 428, 641
StyD4I CCNGG 1 cut(s) 270
TaaI ACNGT 1 cut(s) 512
TaiI ACGT 1 cut(s) 98
TaqI TCGA 2 cut(s) 264, 372
TasI AATT 6 cut(s) 87, 105, 225, 254, 428, 641
TatI WGTACW 2 cut(s) 420, 501
Tru1I TTAA 2 cut(s) 174, 257
Tru9I TTAA 2 cut(s) 174, 257
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 2 cut(s) 319, 485
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 1 cut(s) 577
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 100, 292
VspI ATTAAT 1 cut(s) 257
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 574
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.