RLG00000019067

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012243
Physical Location & Seq
Reverse (-)
45886133 .. 45888142
2010 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019067

Sequence Viewer

Length: 423 bp
ATGGCGCCCAACAACAAAGAGAAGAAGTCCACAAAGCCTCCATTCGTTCCGGCCAAAGATGACAACAAACTTGTGCTTCAAGACCCAATTCTAAGGTCGGACCCAAGTGAGACGGAGGAAGGCGTGCTGAGATTGCCTTCATTTTTTCTATTCCCAGACCATCAAGAAAGGCTTAACACCAAAATAGGGAGGCGGAGGCATTGGCGTTCCTGCCAAGGCGTCGATAAGCATCGACTTGCCGGAACGGCTAGCGTCGAGCACCGCCAGCACTTCGCCCTCGCGAGCCTCGCAGAGATGCCGTGTAGCAGCGTCTTGGTCCTCATGAATAGGCTCTCCCCTCCGACTGGCTTCCACAGCGTCGCCGCGCCGTGGGAGGCGCGTGGGAAATCGTCGAGCTCCATAGTTAAAAACAGTGATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

141

Amino Acids

15.66

Weight (kDa)

9.69

Isoelectric Point (pI)

64.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 3 cut(s) 281, 365, 379
AciI CCGC 3 cut(s) 193, 262, 363
AcoI YGGCCR 1 cut(s) 51
AcyI GRCGYC 2 cut(s) 5, 219
AfiI CCNNNNNNNGG 3 cut(s) 186, 344, 369
AgsI TTSAA 1 cut(s) 80
AluBI AGCT 1 cut(s) 396
AluI AGCT 1 cut(s) 396
Alw21I GWGCWC 2 cut(s) 261, 398
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 306
AspLEI GCGC 3 cut(s) 7, 367, 379
AspS9I GGNCC 2 cut(s) 100, 316
AsuNHI GCTAGC 1 cut(s) 248
AvaII GGWCC 2 cut(s) 100, 316
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 398
Bbv12I GWGCWC 2 cut(s) 261, 398
BbvI GCAGC 1 cut(s) 318
BccI CCATC 1 cut(s) 168
BceAI ACGGC 3 cut(s) 261, 283, 352
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 249
BfoI RGCGCY 1 cut(s) 8
BglI GCCNNNNNGGC 1 cut(s) 245
BisI GCNGC 2 cut(s) 307, 363
BlsI GCNGC 2 cut(s) 308, 364
Bme18I GGWCC 2 cut(s) 100, 316
BmgT120I GGNCC 2 cut(s) 100, 316
BmiI GGNNCC 2 cut(s) 6, 102
BmsI GCATC 2 cut(s) 238, 285
BmtI GCTAGC 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 228
BsaHI GRCGYC 2 cut(s) 5, 219
BsaJI CCNNGG 2 cut(s) 214, 368
BsaXI ACNNNNNCTCC 2 cut(s) 22, 52
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 344, 369
Bse1I ACTGG 1 cut(s) 349
Bse8I GATNNNNATC 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 214, 368
BseJI GATNNNNATC 1 cut(s) 228
BseLI CCNNNNNNNGG 3 cut(s) 186, 344, 369
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 1 cut(s) 349
BseXI GCAGC 1 cut(s) 318
Bsh1236I CGCG 3 cut(s) 281, 365, 379
BshFI GGCC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 261, 398
BsiSI CCGG 2 cut(s) 50, 240
BslI CCNNNNNNNGG 3 cut(s) 186, 344, 369
BsmAI GTCTC 1 cut(s) 104
BsmBI CGTCTC 1 cut(s) 104
BsnI GGCC 1 cut(s) 53
Bsp1286I GDGCHC 2 cut(s) 261, 398
Bsp68I TCGCGA 1 cut(s) 281
BspACI CCGC 3 cut(s) 193, 262, 363
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 3 cut(s) 281, 365, 379
BspHI TCATGA 1 cut(s) 321
BspLI GGNNCC 2 cut(s) 6, 102
BspOI GCTAGC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 214, 368
BssNI GRCGYC 2 cut(s) 5, 219
BssT1I CCWWGG 1 cut(s) 214
Bst4CI ACNGT 1 cut(s) 413
BstACI GRCGYC 2 cut(s) 5, 219
BstC8I GCNNGC 4 cut(s) 125, 250, 266, 283
BstDEI CTNAG 2 cut(s) 92, 128
BstDSI CCRYGG 1 cut(s) 368
BstFNI CGCG 3 cut(s) 281, 365, 379
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 367, 379
BstMAI GTCTC 1 cut(s) 104
BstMWI GCNNNNNNNGC 5 cut(s) 133, 245, 265, 287, 354
BstUI CGCG 3 cut(s) 281, 365, 379
BstV1I GCAGC 1 cut(s) 318
BsuRI GGCC 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 418
BtuMI TCGCGA 1 cut(s) 281
Cac8I GCNNGC 4 cut(s) 125, 250, 266, 283
CciI TCATGA 1 cut(s) 321
CfoI GCGC 3 cut(s) 7, 367, 379
Cfr13I GGNCC 2 cut(s) 100, 316
CseI GACGC 4 cut(s) 208, 241, 298, 346
CviAII CATG 1 cut(s) 322
CviJI RGCY 8 cut(s) 37, 53, 172, 248, 285, 331, 348, 396
CviKI_1 RGCY 8 cut(s) 37, 53, 172, 248, 285, 331, 348, 396
DdeI CTNAG 2 cut(s) 92, 128
DinI GGCGCC 1 cut(s) 6
EaeI YGGCCR 1 cut(s) 51
EciI GGCGGA 1 cut(s) 208
Ecl136II GAGCTC 1 cut(s) 396
Eco130I CCWWGG 1 cut(s) 214
Eco24I GRGCYC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 100, 316
Eco53kI GAGCTC 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 396
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 398
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 214
Esp3I CGTCTC 1 cut(s) 104
FaeI CATG 1 cut(s) 325
FaiI YATR 2 cut(s) 323, 401
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FatI CATG 1 cut(s) 321
Fnu4HI GCNGC 2 cut(s) 307, 363
FriOI GRGCYC 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 307, 363
FspBI CTAG 1 cut(s) 249
GlaI GCGC 3 cut(s) 6, 366, 378
GluI GCNGC 2 cut(s) 307, 363
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 53
HapII CCGG 2 cut(s) 50, 240
HgaI GACGC 4 cut(s) 208, 241, 298, 346
HhaI GCGC 3 cut(s) 7, 367, 379
Hin1I GRCGYC 2 cut(s) 5, 219
Hin1II CATG 1 cut(s) 325
Hin6I GCGC 3 cut(s) 5, 365, 377
HinP1I GCGC 3 cut(s) 5, 365, 377
HpaII CCGG 2 cut(s) 50, 240
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 3 cut(s) 100, 342, 422
Hpy188III TCNNGA 4 cut(s) 80, 164, 280, 322
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 4 cut(s) 224, 257, 362, 394
HpyAV CCTTC 2 cut(s) 113, 147
HpyCH4III ACNGT 1 cut(s) 413
HpyF10VI GCNNNNNNNGC 5 cut(s) 133, 245, 265, 287, 354
HpyF3I CTNAG 2 cut(s) 92, 128
Hsp92I GRCGYC 2 cut(s) 5, 219
Hsp92II CATG 1 cut(s) 325
HspAI GCGC 3 cut(s) 5, 365, 377
KasI GGCGCC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 401
LpnPI CCDG 6 cut(s) 63, 168, 223, 253, 278, 330
Lsp1109I GCAGC 1 cut(s) 318
LweI GCATC 2 cut(s) 238, 285
MaeI CTAG 1 cut(s) 249
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 2 cut(s) 261, 398
MluCI AATT 1 cut(s) 87
Mly113I GGCGCC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 78, 365
MnlI CCTC 9 cut(s) 48, 109, 183, 189, 287, 296, 329, 348, 367
MseI TTAA 2 cut(s) 174, 405
MspI CCGG 2 cut(s) 50, 240
MvnI CGCG 3 cut(s) 281, 365, 379
MwoI GCNNNNNNNGC 5 cut(s) 133, 245, 265, 287, 354
NarI GGCGCC 1 cut(s) 5
NheI GCTAGC 1 cut(s) 248
NlaIII CATG 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 6, 102
NruI TCGCGA 1 cut(s) 281
PagI TCATGA 1 cut(s) 321
PkrI GCNGC 2 cut(s) 308, 364
PluTI GGCGCC 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 6, 102
PspPI GGNCC 2 cut(s) 100, 316
RruI TCGCGA 1 cut(s) 281
SacI GAGCTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 174, 405
SatI GCNGC 2 cut(s) 307, 363
Sau96I GGNCC 2 cut(s) 100, 316
SduI GDGCHC 2 cut(s) 261, 398
SetI ASST 2 cut(s) 98, 398
SfaNI GCATC 2 cut(s) 238, 285
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 2 cut(s) 100, 316
Sse9I AATT 1 cut(s) 87
SsiI CCGC 3 cut(s) 193, 262, 363
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 249
SstI GAGCTC 1 cut(s) 398
StyI CCWWGG 1 cut(s) 214
TaaI ACNGT 1 cut(s) 413
TaqI TCGA 4 cut(s) 222, 232, 255, 392
TasI AATT 1 cut(s) 87
TauI GCSGC 1 cut(s) 365
Tru1I TTAA 2 cut(s) 174, 405
Tru9I TTAA 2 cut(s) 174, 405
TscAI CASTG 1 cut(s) 418
TseI GCWGC 1 cut(s) 306
TspDTI ATGAA 2 cut(s) 129, 338
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 1 cut(s) 418
VpaK11BI GGWCC 2 cut(s) 100, 316
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.