MD03G1055700.v1.1

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013020
Physical Location & Seq
Forward (+)
4431429 .. 4432165
737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1055700.v1.1.491

Sequence Viewer

Length: 537 bp
ATGAACGCCACAGCTCCGCCTGATGTCATCCAAGTCACAGATTACCAGAACGGAAGCGATTCCGACACCAACATCGACGACGATGACGGCGATGCAGCCGCCGCCGAGTTCTACCAGCCAGTCTCCGCCGTCGACTCCGAAGACGACGAAGACCAGATCGGAAACGACGCCGTCCCGATCCACTCCCAGCTGCACTCCAACGGCGTCACCGTCAATCAGATCGACAGAGGAATCTCGTCTCTCCAGCTAAACGACGGCGTCGGGCGCAATGATCGCTTGAACAGCAGCAGCGATGACGAAGCGGAAGCAGAAGAAGTGGAGGAGGAGGTCGCTTCCGACTCGGAGATAATTAGGGCTTTCAGAGAAGACGAGAATCGCAGAAACGCGCCGCTTACGGCGGAGAACGCGACTAGGGTCAGGGAGGCCATGCGTGGAATTTCCTTCGCCGGAGCTCCTCCGCATTGGGCGGGTCTGATCCCCGAGAACAACTGGATGGACCGCCTTCGCGGGCTCGGGCAATCTTCGTCCCGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

179

Amino Acids

19.4

Weight (kDa)

4.13

Isoelectric Point (pI)

48.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 132
AccII CGCG 3 cut(s) 386, 407, 507
AclWI GGATC 2 cut(s) 172, 469
AcsI RAATTY 1 cut(s) 435
AcyI GRCGYC 3 cut(s) 168, 204, 258
AgsI TTSAA 1 cut(s) 280
AluBI AGCT 4 cut(s) 14, 190, 247, 452
AluI AGCT 4 cut(s) 14, 190, 247, 452
Alw21I GWGCWC 1 cut(s) 454
Alw26I GTCTC 2 cut(s) 127, 243
AlwI GGATC 2 cut(s) 172, 469
Ama87I CYCGRG 2 cut(s) 479, 512
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 4 cut(s) 95, 190, 285, 288
ApoI RAATTY 1 cut(s) 435
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 267, 388
AspS9I GGNCC 1 cut(s) 496
AsuC2I CCSGG 1 cut(s) 529
AsuHPI GGTGA 1 cut(s) 199
AvaI CYCGRG 2 cut(s) 479, 512
AvaII GGWCC 1 cut(s) 496
BanII GRGCYC 2 cut(s) 454, 513
BbsI GAAGAC 3 cut(s) 147, 156, 372
Bbv12I GWGCWC 1 cut(s) 454
BbvI GCAGC 4 cut(s) 107, 177, 297, 300
BccI CCATC 1 cut(s) 487
BceAI ACGGC 6 cut(s) 103, 113, 155, 217, 271, 411
BcnI CCSGG 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 127, 243
BfaI CTAG 1 cut(s) 411
BisI GCNGC 7 cut(s) 96, 99, 102, 191, 286, 289, 389
BlsI GCNGC 7 cut(s) 97, 100, 103, 192, 287, 290, 390
Bme1390I CCNGG 1 cut(s) 529
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 2 cut(s) 479, 512
BmgT120I GGNCC 1 cut(s) 496
BmrFI CCNGG 1 cut(s) 529
BmsI GCATC 1 cut(s) 82
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 3 cut(s) 147, 156, 372
BpmI CTGGAG 1 cut(s) 227
BpuMI CCSGG 1 cut(s) 529
BsaHI GRCGYC 3 cut(s) 168, 204, 258
Bse1I ACTGG 2 cut(s) 119, 494
Bse3DI GCAATG 1 cut(s) 274
BseGI GGATG 2 cut(s) 27, 498
BseMI GCAATG 1 cut(s) 274
BseNI ACTGG 2 cut(s) 119, 494
BseRI GAGGAG 3 cut(s) 335, 338, 444
BseXI GCAGC 4 cut(s) 107, 177, 297, 300
BseYI CCCAGC 1 cut(s) 186
BsgI GTGCAG 1 cut(s) 176
Bsh1236I CGCG 3 cut(s) 386, 407, 507
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 454
BsiHKCI CYCGRG 2 cut(s) 479, 512
BsiSI CCGG 2 cut(s) 447, 529
BslFI GGGAC 2 cut(s) 158, 511
BsmAI GTCTC 2 cut(s) 127, 243
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 2 cut(s) 158, 511
BsnI GGCC 1 cut(s) 425
BsoBI CYCGRG 2 cut(s) 479, 512
Bsp1286I GDGCHC 2 cut(s) 454, 513
Bsp143I GATC 5 cut(s) 156, 177, 219, 271, 474
BspANI GGCC 1 cut(s) 425
BspFNI CGCG 3 cut(s) 386, 407, 507
BspPI GGATC 2 cut(s) 172, 469
BsrDI GCAATG 1 cut(s) 274
BsrI ACTGG 2 cut(s) 119, 494
BssMI GATC 5 cut(s) 156, 177, 219, 271, 474
BssNI GRCGYC 3 cut(s) 168, 204, 258
Bst4CI ACNGT 1 cut(s) 211
BstACI GRCGYC 3 cut(s) 168, 204, 258
BstC8I GCNNGC 1 cut(s) 509
BstF5I GGATG 2 cut(s) 27, 498
BstFNI CGCG 3 cut(s) 386, 407, 507
BstHHI GCGC 2 cut(s) 267, 388
BstKTI GATC 5 cut(s) 159, 180, 222, 274, 477
BstMAI GTCTC 2 cut(s) 127, 243
BstMBI GATC 5 cut(s) 156, 177, 219, 271, 474
BstMWI GCNNNNNNNGC 5 cut(s) 101, 264, 273, 282, 404
BstPAI GACNNNNGTC 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 527
BstUI CGCG 3 cut(s) 386, 407, 507
BstV1I GCAGC 4 cut(s) 107, 177, 297, 300
BstV2I GAAGAC 3 cut(s) 147, 156, 372
BsuRI GGCC 1 cut(s) 425
BtgZI GCGATG 2 cut(s) 105, 306
BtsCI GGATG 2 cut(s) 27, 498
Cac8I GCNNGC 1 cut(s) 509
CfoI GCGC 2 cut(s) 267, 388
Cfr13I GGNCC 1 cut(s) 496
CseI GACGC 3 cut(s) 176, 193, 247
CviAII CATG 1 cut(s) 427
CviJI RGCY 9 cut(s) 14, 98, 118, 190, 247, 356, 425, 452, 511
CviKI_1 RGCY 9 cut(s) 14, 98, 118, 190, 247, 356, 425, 452, 511
DpnI GATC 5 cut(s) 158, 179, 221, 273, 476
DpnII GATC 5 cut(s) 156, 177, 219, 271, 474
EciI GGCGGA 3 cut(s) 6, 115, 413
Ecl136II GAGCTC 1 cut(s) 452
Eco24I GRGCYC 2 cut(s) 454, 513
Eco47I GGWCC 1 cut(s) 496
Eco53kI GAGCTC 1 cut(s) 452
Eco88I CYCGRG 2 cut(s) 479, 512
EcoICRI GAGCTC 1 cut(s) 452
EcoT38I GRGCYC 2 cut(s) 454, 513
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 1 cut(s) 430
FaiI YATR 1 cut(s) 428
FaqI GGGAC 2 cut(s) 158, 511
FatI CATG 1 cut(s) 426
FauI CCCGC 2 cut(s) 460, 500
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 7 cut(s) 96, 99, 102, 191, 286, 289, 389
FokI GGATG 2 cut(s) 14, 505
FriOI GRGCYC 2 cut(s) 454, 513
Fsp4HI GCNGC 7 cut(s) 96, 99, 102, 191, 286, 289, 389
FspBI CTAG 1 cut(s) 411
GlaI GCGC 2 cut(s) 266, 387
GluI GCNGC 7 cut(s) 96, 99, 102, 191, 286, 289, 389
GsaI CCCAGC 1 cut(s) 190
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 1 cut(s) 425
HapII CCGG 2 cut(s) 447, 529
HgaI GACGC 3 cut(s) 176, 193, 247
HhaI GCGC 2 cut(s) 267, 388
Hin1I GRCGYC 3 cut(s) 168, 204, 258
Hin1II CATG 1 cut(s) 430
Hin6I GCGC 2 cut(s) 265, 386
HinP1I GCGC 2 cut(s) 265, 386
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 5 cut(s) 59, 134, 231, 338, 373
HpaII CCGG 2 cut(s) 447, 529
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 8 cut(s) 64, 139, 161, 219, 337, 343, 362, 474
Hpy188III TCNNGA 1 cut(s) 175
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 7 cut(s) 80, 83, 134, 149, 170, 257, 263
HpyAV CCTTC 2 cut(s) 451, 512
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 2 cut(s) 95, 193
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 264, 273, 282, 404
Hsp92I GRCGYC 3 cut(s) 168, 204, 258
Hsp92II CATG 1 cut(s) 430
HspAI GCGC 2 cut(s) 265, 386
Kzo9I GATC 5 cut(s) 156, 177, 219, 271, 474
LmnI GCTCC 3 cut(s) 19, 449, 457
Lsp1109I GCAGC 4 cut(s) 107, 177, 297, 300
LweI GCATC 1 cut(s) 82
MaeI CTAG 1 cut(s) 411
MaeIII GTNAC 2 cut(s) 34, 205
MalI GATC 5 cut(s) 158, 179, 221, 273, 476
MboI GATC 5 cut(s) 156, 177, 219, 271, 474
MboII GAAGA 5 cut(s) 152, 161, 323, 377, 513
MhlI GDGCHC 2 cut(s) 454, 513
MluCI AATT 2 cut(s) 348, 435
MlyI GAGTC 2 cut(s) 128, 332
MmeI TCCRAC 3 cut(s) 87, 222, 360
MnlI CCTC 7 cut(s) 221, 313, 316, 319, 415, 465, 525
MroXI GAANNNNTTC 1 cut(s) 58
MspA1I CMGCKG 1 cut(s) 190
MspI CCGG 2 cut(s) 447, 529
MspR9I CCNGG 1 cut(s) 529
MvnI CGCG 3 cut(s) 386, 407, 507
MwoI GCNNNNNNNGC 5 cut(s) 101, 264, 273, 282, 404
NciI CCSGG 1 cut(s) 529
NdeII GATC 5 cut(s) 156, 177, 219, 271, 474
NlaIII CATG 1 cut(s) 430
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 34, 205
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 207
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 3 cut(s) 59, 231, 373
PflFI GACNNNGTC 2 cut(s) 170, 257
PfoI TCCNGGA 1 cut(s) 527
PkrI GCNGC 7 cut(s) 97, 100, 103, 192, 287, 290, 390
PleI GAGTC 2 cut(s) 128, 332
PpsI GAGTC 2 cut(s) 128, 332
PshAI GACNNNNGTC 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 454
PspFI CCCAGC 1 cut(s) 186
PspPI GGNCC 1 cut(s) 496
PsyI GACNNNGTC 2 cut(s) 170, 257
PvuII CAGCTG 1 cut(s) 190
SacI GAGCTC 1 cut(s) 454
SalI GTCGAC 1 cut(s) 131
SatI GCNGC 7 cut(s) 96, 99, 102, 191, 286, 289, 389
Sau3AI GATC 5 cut(s) 156, 177, 219, 271, 474
Sau96I GGNCC 1 cut(s) 496
SchI GAGTC 2 cut(s) 128, 332
ScrFI CCNGG 1 cut(s) 529
SduI GDGCHC 2 cut(s) 454, 513
SetI ASST 6 cut(s) 16, 192, 249, 330, 454, 536
SfaNI GCATC 1 cut(s) 82
SinI GGWCC 1 cut(s) 496
Sse9I AATT 2 cut(s) 348, 435
SspMI CTAG 1 cut(s) 411
SstI GAGCTC 1 cut(s) 454
StyD4I CCNGG 1 cut(s) 527
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 3 cut(s) 75, 132, 222
TasI AATT 2 cut(s) 348, 435
TauI GCSGC 3 cut(s) 101, 104, 391
TfiI GAWTC 3 cut(s) 59, 231, 373
TseFI GTSAC 2 cut(s) 34, 205
TseI GCWGC 4 cut(s) 95, 190, 285, 288
Tsp45I GTSAC 2 cut(s) 34, 205
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 66
Tth111I GACNNNGTC 2 cut(s) 170, 257
VpaK11BI GGWCC 1 cut(s) 496
XapI RAATTY 1 cut(s) 435
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 58
XspI CTAG 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.