Rroxscaffold_1G00009610

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013020
Physical Location & Seq
Reverse (-)
12157188 .. 12160769
3582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009610.1

Sequence Viewer

Length: 498 bp
ATGAAAGCGCGGCTCAATGTGTCGAAATCCGGTATAGAATGCGGCTTGAGATCTTGGAACCATGTCGACGCCGCCCCGTACCCCCCTCCCCCTTTTTCCTCAAGCCGACGACATGCGATCCTTGCACGTAGTCTTACTGGTATAAATAGCCTTGGAAATGGGCATATTGCGGTTCCTATTGGCTCATGCGGAAGCGATTCCGACACCAACATCGACGACGACGATGCCGCCGCCGAGTACTACCAGCCAGTCTCCGCCTTGCAGCGAAGAAGAAGAAGAAGATGTGATTGGAGGGGTTCTGATTCGGCGATCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGTCACGCGCCGTTGACGCCGGAGAATGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCGGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

165

Amino Acids

18.43

Weight (kDa)

9.34

Isoelectric Point (pI)

92.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 99 - 158 1.7e-06 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 66
AccII CGCG 2 cut(s) 10, 350
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 9 cut(s) 10, 42, 72, 170, 189, 228, 231, 255, 431
AclWI GGATC 5 cut(s) 112, 433, 441, 446, 464
AcuI CTGAAG 1 cut(s) 465
AcyI GRCGYC 2 cut(s) 69, 359
AfaI GTAC 2 cut(s) 80, 239
AjuI GAANNNNNNNTTGG 2 cut(s) 271, 303
Alw26I GTCTC 1 cut(s) 256
AlwI GGATC 5 cut(s) 112, 433, 441, 446, 464
Aor13HI TCCGGA 1 cut(s) 421
ApeKI GCWGC 1 cut(s) 262
Asp700I GAANNNNTTC 1 cut(s) 196
AspLEI GCGC 2 cut(s) 10, 352
AsuHPI GGTGA 1 cut(s) 477
BamHI GGATCC 1 cut(s) 438
BauI CACGAG 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 274
BceAI ACGGC 2 cut(s) 327, 337
BcgI CGANNNNNNTGC 2 cut(s) 206, 240
BcoDI GTCTC 1 cut(s) 256
BglI GCCNNNNNGGC 1 cut(s) 416
BglII AGATCT 1 cut(s) 50
BisI GCNGC 6 cut(s) 11, 43, 72, 228, 231, 263
BlsI GCNGC 6 cut(s) 12, 44, 73, 229, 232, 264
BmcAI AGTACT 1 cut(s) 239
BmiI GGNNCC 5 cut(s) 59, 174, 420, 440, 476
BmsI GCATC 1 cut(s) 214
BoxI GACNNNNGTC 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 67, 85
Bsa29I ATCGAT 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 128
BsaHI GRCGYC 2 cut(s) 69, 359
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 2 cut(s) 29, 421
Bse118I RCCGGY 1 cut(s) 471
Bse1I ACTGG 3 cut(s) 142, 248, 458
BseAI TCCGGA 1 cut(s) 421
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 151
BseNI ACTGG 3 cut(s) 142, 248, 458
BseXI GCAGC 1 cut(s) 274
Bsh1236I CGCG 2 cut(s) 10, 350
Bsh1285I CGRYCG 1 cut(s) 463
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 1 cut(s) 463
BsiSI CCGG 5 cut(s) 30, 362, 411, 422, 472
BsmAI GTCTC 1 cut(s) 256
BsmI GAATGC 2 cut(s) 44, 373
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
BspACI CCGC 9 cut(s) 10, 42, 72, 170, 189, 228, 231, 255, 431
BspDI ATCGAT 1 cut(s) 459
BspEI TCCGGA 1 cut(s) 421
BspFNI CGCG 2 cut(s) 10, 350
BspLI GGNNCC 5 cut(s) 59, 174, 420, 440, 476
BspPI GGATC 5 cut(s) 112, 433, 441, 446, 464
BsrFI RCCGGY 1 cut(s) 471
BsrI ACTGG 3 cut(s) 142, 248, 458
BssAI RCCGGY 1 cut(s) 471
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
BssNI GRCGYC 2 cut(s) 69, 359
BssSI CACGAG 1 cut(s) 313
BssT1I CCWWGG 1 cut(s) 151
Bst2BI CACGAG 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 483
BstACI GRCGYC 2 cut(s) 69, 359
BstBAI YACGTR 1 cut(s) 128
BstC8I GCNNGC 1 cut(s) 473
BstFNI CGCG 2 cut(s) 10, 350
BstHHI GCGC 2 cut(s) 10, 352
BstKTI GATC 8 cut(s) 53, 120, 312, 324, 436, 441, 459, 463
BstMAI GTCTC 1 cut(s) 256
BstMBI GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 4 cut(s) 122, 358, 416, 472
BstNSI RCATGY 1 cut(s) 116
BstPAI GACNNNNGTC 1 cut(s) 336
BstUI CGCG 2 cut(s) 10, 350
BstV1I GCAGC 1 cut(s) 274
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 2 cut(s) 50, 438
BstYI RGATCY 2 cut(s) 50, 438
Bsu15I ATCGAT 1 cut(s) 459
BsuTUI ATCGAT 1 cut(s) 459
BtgZI GCGATG 1 cut(s) 404
Cac8I GCNNGC 1 cut(s) 473
CfoI GCGC 2 cut(s) 10, 352
Cfr10I RCCGGY 1 cut(s) 471
ClaI ATCGAT 1 cut(s) 459
CseI GACGC 2 cut(s) 77, 367
Csp6I GTAC 2 cut(s) 79, 238
CviAII CATG 3 cut(s) 62, 113, 186
CviJI RGCY 9 cut(s) 13, 45, 105, 150, 183, 247, 419, 466, 475
CviKI_1 RGCY 9 cut(s) 13, 45, 105, 150, 183, 247, 419, 466, 475
CviQI GTAC 2 cut(s) 79, 238
DpnI GATC 8 cut(s) 52, 119, 311, 323, 435, 440, 458, 462
DpnII GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
EciI GGCGGA 2 cut(s) 244, 446
Eco130I CCWWGG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 3 cut(s) 65, 116, 189
FaiI YATR 7 cut(s) 35, 63, 114, 143, 165, 187, 383
FatI CATG 3 cut(s) 61, 112, 185
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 6 cut(s) 11, 43, 72, 228, 231, 263
Fsp4HI GCNGC 6 cut(s) 11, 43, 72, 228, 231, 263
GlaI GCGC 2 cut(s) 9, 351
GluI GCNGC 6 cut(s) 11, 43, 72, 228, 231, 263
HapII CCGG 5 cut(s) 30, 362, 411, 422, 472
HgaI GACGC 2 cut(s) 77, 367
HhaI GCGC 2 cut(s) 10, 352
Hin1I GRCGYC 2 cut(s) 69, 359
Hin1II CATG 3 cut(s) 65, 116, 189
Hin6I GCGC 2 cut(s) 8, 350
HinP1I GCGC 2 cut(s) 8, 350
HincII GTYRAC 2 cut(s) 67, 357
HindII GTYRAC 2 cut(s) 67, 357
HinfI GANTC 3 cut(s) 197, 302, 337
HpaII CCGG 5 cut(s) 30, 362, 411, 422, 472
HphI GGTGA 1 cut(s) 477
Hpy166II GTNNAC 2 cut(s) 67, 357
Hpy188I TCNGA 5 cut(s) 202, 301, 326, 438, 479
Hpy188III TCNNGA 2 cut(s) 313, 422
Hpy8I GTNNAC 2 cut(s) 67, 357
Hpy99I CGWCG 6 cut(s) 71, 111, 218, 221, 224, 376
HpyCH4III ACNGT 1 cut(s) 483
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 2 cut(s) 125, 262
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 358, 416, 472
HpySE526I ACGT 1 cut(s) 127
Hsp92I GRCGYC 2 cut(s) 69, 359
Hsp92II CATG 3 cut(s) 65, 116, 189
HspAI GCGC 2 cut(s) 8, 350
Kpn2I TCCGGA 1 cut(s) 421
KroI GCCGGC 1 cut(s) 471
KroNI GCCGGC 1 cut(s) 473
Kzo9I GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
LmnI GCTCC 2 cut(s) 424, 480
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 214
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 344, 483
MalI GATC 8 cut(s) 52, 119, 311, 323, 435, 440, 458, 462
MboI GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
MboII GAAGA 7 cut(s) 279, 282, 285, 288, 291, 341, 458
MflI RGATCY 2 cut(s) 50, 438
MlyI GAGTC 1 cut(s) 346
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 7 cut(s) 96, 109, 285, 309, 369, 379, 389
MroI TCCGGA 1 cut(s) 421
MroNI GCCGGC 1 cut(s) 471
MroXI GAANNNNTTC 1 cut(s) 196
MseI TTAA 1 cut(s) 496
MspI CCGG 5 cut(s) 30, 362, 411, 422, 472
Mva1269I GAATGC 2 cut(s) 44, 373
MvnI CGCG 2 cut(s) 10, 350
MwoI GCNNNNNNNGC 4 cut(s) 122, 358, 416, 472
NaeI GCCGGC 1 cut(s) 473
NdeII GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
NgoMIV GCCGGC 1 cut(s) 471
NlaIII CATG 3 cut(s) 65, 116, 189
NlaIV GGNNCC 5 cut(s) 59, 174, 420, 440, 476
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 2 cut(s) 344, 483
NspI RCATGY 1 cut(s) 116
PcsI WCGNNNNNNNCGW 3 cut(s) 219, 311, 475
PctI GAATGC 2 cut(s) 44, 373
PdiI GCCGGC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 197, 302
PkrI GCNGC 6 cut(s) 12, 44, 73, 229, 232, 264
Ple19I CGATCG 1 cut(s) 463
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
Ppu21I YACGTR 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 336
PspN4I GGNNCC 5 cut(s) 59, 174, 420, 440, 476
PsuI RGATCY 2 cut(s) 50, 438
PvuI CGATCG 1 cut(s) 463
RsaI GTAC 2 cut(s) 80, 239
RsaNI GTAC 2 cut(s) 79, 238
SalI GTCGAC 1 cut(s) 65
SaqAI TTAA 1 cut(s) 496
SatI GCNGC 6 cut(s) 11, 43, 72, 228, 231, 263
Sau3AI GATC 8 cut(s) 50, 117, 309, 321, 433, 438, 456, 460
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 1 cut(s) 346
SetI ASST 1 cut(s) 130
SfaNI GCATC 1 cut(s) 214
SmlI CTYRAG 2 cut(s) 46, 100
SmoI CTYRAG 2 cut(s) 46, 100
SsiI CCGC 9 cut(s) 10, 42, 72, 170, 189, 228, 231, 255, 431
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 483
TaiI ACGT 1 cut(s) 130
TaqI TCGA 4 cut(s) 23, 66, 213, 459
TatI WGTACW 1 cut(s) 237
TauI GCSGC 5 cut(s) 13, 45, 74, 230, 233
TfiI GAWTC 2 cut(s) 197, 302
Tru1I TTAA 1 cut(s) 496
Tru9I TTAA 1 cut(s) 496
TseFI GTSAC 2 cut(s) 344, 483
TseI GCWGC 1 cut(s) 262
Tsp45I GTSAC 2 cut(s) 344, 483
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 196
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.