Rh5AG469100

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013020
Physical Location & Seq
Forward (+)
80451739 .. 80452737
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG469100.1

Sequence Viewer

Length: 525 bp
ATGAACGCTACTACCTCTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAGCGGAAGCGATTCCGACACCAACATCGACGACGACGACGACGCCGCCGACGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGCAGCGAAGAAGAAGAAGAAGATGTGATTGGAGTGGGTTCTGATTCGGCGATCACGAGGGCGATCAGGGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGACTGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCCGATCGGATCCCTGAAGAAAACTGGATTGATCGACTTCGCCGGCTCCGGCAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.18

Weight (kDa)

4.14

Isoelectric Point (pI)

60.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 35 - 167 1.6e-06 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 267
AccII CGCG 1 cut(s) 377
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 3 cut(s) 54, 96, 123
AclWI GGATC 2 cut(s) 460, 473
AcuI CTGAAG 1 cut(s) 492
AcyI GRCGYC 4 cut(s) 93, 171, 264, 386
AfaI GTAC 1 cut(s) 107
AjnI CCWGG 1 cut(s) 222
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw26I GTCTC 2 cut(s) 124, 386
AlwI GGATC 2 cut(s) 460, 473
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 193, 196, 288
Asp700I GAANNNNTTC 1 cut(s) 61
AspLEI GCGC 2 cut(s) 229, 379
AspS9I GGNCC 1 cut(s) 456
AsuHPI GGTGA 3 cut(s) 146, 284, 504
BamHI GGATCC 1 cut(s) 465
BauI CACGAG 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 144, 363
BbvI GCAGC 3 cut(s) 183, 205, 300
BccI CCATC 1 cut(s) 165
BceAI ACGGC 5 cut(s) 23, 110, 158, 223, 364
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 124, 386
BglI GCCNNNNNGGC 1 cut(s) 443
BisI GCNGC 4 cut(s) 96, 194, 197, 289
BlsI GCNGC 4 cut(s) 97, 195, 198, 290
BmcAI AGTACT 1 cut(s) 107
Bme1390I CCNGG 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 456
BmiI GGNNCC 3 cut(s) 447, 467, 503
BmrFI CCNGG 1 cut(s) 224
BoxI GACNNNNGTC 1 cut(s) 363
BpiI GAAGAC 2 cut(s) 144, 363
BsaHI GRCGYC 4 cut(s) 93, 171, 264, 386
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bse118I RCCGGY 2 cut(s) 369, 498
Bse1I ACTGG 2 cut(s) 116, 485
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 1 cut(s) 224
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 2 cut(s) 116, 485
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 3 cut(s) 183, 205, 300
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 463
BshFI GGCC 1 cut(s) 458
BsiEI CGRYCG 1 cut(s) 463
BsiSI CCGG 6 cut(s) 370, 389, 438, 449, 499, 505
BsmAI GTCTC 2 cut(s) 124, 386
BsnI GGCC 1 cut(s) 458
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 5 cut(s) 336, 348, 460, 465, 487
BspACI CCGC 3 cut(s) 54, 96, 123
BspANI GGCC 1 cut(s) 458
BspCNI CTCAG 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 448
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 447, 467, 503
BspPI GGATC 2 cut(s) 460, 473
BsrFI RCCGGY 2 cut(s) 369, 498
BsrI ACTGG 2 cut(s) 116, 485
BssAI RCCGGY 2 cut(s) 369, 498
BssMI GATC 5 cut(s) 336, 348, 460, 465, 487
BssNI GRCGYC 4 cut(s) 93, 171, 264, 386
BssSI CACGAG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 224
BstACI GRCGYC 4 cut(s) 93, 171, 264, 386
BstC8I GCNNGC 3 cut(s) 371, 375, 500
BstDEI CTNAG 1 cut(s) 248
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 2 cut(s) 229, 379
BstKTI GATC 5 cut(s) 339, 351, 463, 468, 490
BstMAI GTCTC 2 cut(s) 124, 386
BstMBI GATC 5 cut(s) 336, 348, 460, 465, 487
BstMCI CGRYCG 1 cut(s) 463
BstMWI GCNNNNNNNGC 3 cut(s) 288, 385, 443
BstNI CCWGG 1 cut(s) 224
BstPAI GACNNNNGTC 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 222
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 3 cut(s) 183, 205, 300
BstV2I GAAGAC 2 cut(s) 144, 363
BstX2I RGATCY 1 cut(s) 465
BstYI RGATCY 1 cut(s) 465
BsuRI GGCC 1 cut(s) 458
BtgZI GCGATG 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 371, 375, 500
CfoI GCGC 2 cut(s) 229, 379
Cfr10I RCCGGY 2 cut(s) 369, 498
Cfr13I GGNCC 1 cut(s) 456
CseI GACGC 3 cut(s) 101, 179, 394
Csp6I GTAC 1 cut(s) 106
CviAII CATG 1 cut(s) 31
CviJI RGCY 6 cut(s) 38, 115, 196, 446, 458, 502
CviKI_1 RGCY 6 cut(s) 38, 115, 196, 446, 458, 502
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 1 cut(s) 248
DpnI GATC 5 cut(s) 338, 350, 462, 467, 489
DpnII GATC 5 cut(s) 336, 348, 460, 465, 487
EciI GGCGGA 1 cut(s) 112
Eco57I CTGAAG 1 cut(s) 492
EcoRII CCWGG 1 cut(s) 222
FaeI CATG 1 cut(s) 34
FaiI YATR 2 cut(s) 32, 410
FatI CATG 1 cut(s) 30
Fnu4HI GCNGC 4 cut(s) 96, 194, 197, 289
Fsp4HI GCNGC 4 cut(s) 96, 194, 197, 289
GlaI GCGC 2 cut(s) 228, 378
GluI GCNGC 4 cut(s) 96, 194, 197, 289
HaeIII GGCC 1 cut(s) 458
HapII CCGG 6 cut(s) 370, 389, 438, 449, 499, 505
HgaI GACGC 3 cut(s) 101, 179, 394
HhaI GCGC 2 cut(s) 229, 379
Hin1I GRCGYC 4 cut(s) 93, 171, 264, 386
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 2 cut(s) 227, 377
HinP1I GCGC 2 cut(s) 227, 377
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 6 cut(s) 46, 62, 131, 151, 329, 364
HpaII CCGG 6 cut(s) 370, 389, 438, 449, 499, 505
HphI GGTGA 3 cut(s) 146, 284, 504
Hpy166II GTNNAC 2 cut(s) 220, 384
Hpy188I TCNGA 4 cut(s) 67, 136, 328, 465
Hpy188III TCNNGA 2 cut(s) 340, 449
Hpy8I GTNNAC 2 cut(s) 220, 384
HpyCH4IV ACGT 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 385, 443
HpyF3I CTNAG 1 cut(s) 248
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 4 cut(s) 93, 171, 264, 386
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 2 cut(s) 227, 377
Kpn2I TCCGGA 1 cut(s) 448
KroI GCCGGC 2 cut(s) 369, 498
KroNI GCCGGC 2 cut(s) 371, 500
Kzo9I GATC 5 cut(s) 336, 348, 460, 465, 487
LmnI GCTCC 3 cut(s) 204, 451, 507
Lsp1109I GCAGC 3 cut(s) 183, 205, 300
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 510
MalI GATC 5 cut(s) 338, 350, 462, 467, 489
MboI GATC 5 cut(s) 336, 348, 460, 465, 487
MboII GAAGA 8 cut(s) 149, 305, 308, 311, 314, 317, 368, 485
MflI RGATCY 1 cut(s) 465
MlyI GAGTC 1 cut(s) 373
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 8 cut(s) 25, 139, 225, 262, 336, 396, 406, 416
MroI TCCGGA 1 cut(s) 448
MroNI GCCGGC 2 cut(s) 369, 498
MroXI GAANNNNTTC 1 cut(s) 61
MseI TTAA 2 cut(s) 254, 523
MspA1I CMGCKG 2 cut(s) 54, 196
MspI CCGG 6 cut(s) 370, 389, 438, 449, 499, 505
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 288, 385, 443
NaeI GCCGGC 2 cut(s) 371, 500
NdeII GATC 5 cut(s) 336, 348, 460, 465, 487
NgoMIV GCCGGC 2 cut(s) 369, 498
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 3 cut(s) 447, 467, 503
NmuCI GTSAC 1 cut(s) 510
PcsI WCGNNNNNNNCGW 6 cut(s) 84, 87, 96, 99, 264, 338
PdiI GCCGGC 2 cut(s) 371, 500
PdmI GAANNNNTTC 1 cut(s) 61
PfeI GAWTC 5 cut(s) 46, 62, 131, 151, 329
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 4 cut(s) 97, 195, 198, 290
Ple19I CGATCG 1 cut(s) 463
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PshAI GACNNNNGTC 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 3 cut(s) 447, 467, 503
PspPI GGNCC 1 cut(s) 456
PsuI RGATCY 1 cut(s) 465
PsyI GACNNNGTC 1 cut(s) 263
PvuI CGATCG 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SaqAI TTAA 2 cut(s) 254, 523
SatI GCNGC 4 cut(s) 96, 194, 197, 289
Sau3AI GATC 5 cut(s) 336, 348, 460, 465, 487
Sau96I GGNCC 1 cut(s) 456
ScaI AGTACT 1 cut(s) 107
SchI GAGTC 1 cut(s) 373
ScrFI CCNGG 1 cut(s) 224
SetI ASST 3 cut(s) 17, 198, 267
SsiI CCGC 3 cut(s) 54, 96, 123
StyD4I CCNGG 1 cut(s) 222
TaiI ACGT 1 cut(s) 267
TaqI TCGA 4 cut(s) 78, 129, 267, 490
TatI WGTACW 1 cut(s) 105
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 5 cut(s) 46, 62, 131, 151, 329
Tru1I TTAA 2 cut(s) 254, 523
Tru9I TTAA 2 cut(s) 254, 523
TseFI GTSAC 1 cut(s) 510
TseI GCWGC 3 cut(s) 193, 196, 288
Tsp45I GTSAC 1 cut(s) 510
TspDTI ATGAA 2 cut(s) 17, 19
Tth111I GACNNNGTC 1 cut(s) 263
XmnI GAANNNNTTC 1 cut(s) 61
ZraI GACGTC 1 cut(s) 265
ZrmI AGTACT 1 cut(s) 107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.