Rw5G043590

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013020
Physical Location & Seq
Forward (+)
76479082 .. 76481380
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G043590.1

Sequence Viewer

Length: 549 bp
ATGTTGCCCATTGTGAGACTGACAACCATGAACGCTACCTCTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAGCGGAAGCGATTCCGACACCAACATCGACGACGACGACGATGCCGCCGACGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGAAGCGAAGAAGAAGAAGAAGATGTGATTGGAGTGGGTTCTGATTCGGCGATCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGACTGCGACGAGGGTTATGGAGGCGATGACAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCCGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.12

Weight (kDa)

4.19

Isoelectric Point (pI)

60.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 43 - 175 2.1e-06 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 291
AccII CGCG 1 cut(s) 401
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 3 cut(s) 78, 120, 147
AclWI GGATC 3 cut(s) 484, 497, 515
AcuI CTGAAG 1 cut(s) 516
AcyI GRCGYC 3 cut(s) 195, 288, 410
AfaI GTAC 1 cut(s) 131
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AluBI AGCT 1 cut(s) 220
AluI AGCT 1 cut(s) 220
Alw26I GTCTC 3 cut(s) 10, 148, 410
AlwI GGATC 3 cut(s) 484, 497, 515
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 1 cut(s) 480
ApeKI GCWGC 2 cut(s) 217, 220
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
Asp700I GAANNNNTTC 1 cut(s) 85
AspLEI GCGC 2 cut(s) 253, 403
AspS9I GGNCC 1 cut(s) 480
AsuHPI GGTGA 3 cut(s) 170, 308, 528
BamHI GGATCC 1 cut(s) 489
BauI CACGAG 1 cut(s) 364
BbsI GAAGAC 2 cut(s) 168, 387
BbvI GCAGC 2 cut(s) 207, 229
BccI CCATC 1 cut(s) 189
BceAI ACGGC 5 cut(s) 47, 134, 182, 247, 388
BcgI CGANNNNNNTGC 2 cut(s) 98, 132
BciT130I CCWGG 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 10, 148, 410
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 3 cut(s) 120, 218, 221
BlsI GCNGC 3 cut(s) 121, 219, 222
BmcAI AGTACT 1 cut(s) 131
Bme1390I CCNGG 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 3 cut(s) 471, 491, 527
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 106
BoxI GACNNNNGTC 1 cut(s) 387
BpiI GAAGAC 2 cut(s) 168, 387
Bsa29I ATCGAT 1 cut(s) 510
BsaHI GRCGYC 3 cut(s) 195, 288, 410
BsaWI WCCGGW 1 cut(s) 472
Bse118I RCCGGY 2 cut(s) 393, 522
Bse1I ACTGG 2 cut(s) 140, 509
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 248
BseCI ATCGAT 1 cut(s) 510
BseMII CTCAG 1 cut(s) 263
BseNI ACTGG 2 cut(s) 140, 509
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 207, 229
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 2 cut(s) 487, 514
BshFI GGCC 1 cut(s) 482
BshVI ATCGAT 1 cut(s) 510
BsiEI CGRYCG 2 cut(s) 487, 514
BsiSI CCGG 5 cut(s) 394, 413, 462, 473, 523
BsmAI GTCTC 3 cut(s) 10, 148, 410
BsnI GGCC 1 cut(s) 482
Bsp13I TCCGGA 1 cut(s) 472
Bsp143I GATC 6 cut(s) 360, 372, 484, 489, 507, 511
BspACI CCGC 3 cut(s) 78, 120, 147
BspANI GGCC 1 cut(s) 482
BspCNI CTCAG 1 cut(s) 264
BspDI ATCGAT 1 cut(s) 510
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 1 cut(s) 401
BspLI GGNNCC 3 cut(s) 471, 491, 527
BspPI GGATC 3 cut(s) 484, 497, 515
BsrFI RCCGGY 2 cut(s) 393, 522
BsrI ACTGG 2 cut(s) 140, 509
BssAI RCCGGY 2 cut(s) 393, 522
BssMI GATC 6 cut(s) 360, 372, 484, 489, 507, 511
BssNI GRCGYC 3 cut(s) 195, 288, 410
BssSI CACGAG 1 cut(s) 364
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 534
BstACI GRCGYC 3 cut(s) 195, 288, 410
BstC8I GCNNGC 3 cut(s) 395, 399, 524
BstDEI CTNAG 1 cut(s) 272
BstFNI CGCG 1 cut(s) 401
BstHHI GCGC 2 cut(s) 253, 403
BstKTI GATC 6 cut(s) 363, 375, 487, 492, 510, 514
BstMAI GTCTC 3 cut(s) 10, 148, 410
BstMBI GATC 6 cut(s) 360, 372, 484, 489, 507, 511
BstMCI CGRYCG 2 cut(s) 487, 514
BstMWI GCNNNNNNNGC 4 cut(s) 312, 409, 467, 523
BstNI CCWGG 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 1 cut(s) 246
BstUI CGCG 1 cut(s) 401
BstV1I GCAGC 2 cut(s) 207, 229
BstV2I GAAGAC 2 cut(s) 168, 387
BstX2I RGATCY 1 cut(s) 489
BstYI RGATCY 1 cut(s) 489
Bsu15I ATCGAT 1 cut(s) 510
BsuRI GGCC 1 cut(s) 482
BsuTUI ATCGAT 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 455
Cac8I GCNNGC 3 cut(s) 395, 399, 524
CfoI GCGC 2 cut(s) 253, 403
Cfr10I RCCGGY 2 cut(s) 393, 522
Cfr13I GGNCC 1 cut(s) 480
ClaI ATCGAT 1 cut(s) 510
CseI GACGC 2 cut(s) 203, 418
Csp6I GTAC 1 cut(s) 130
CviAII CATG 2 cut(s) 28, 55
CviJI RGCY 7 cut(s) 62, 139, 220, 470, 482, 517, 526
CviKI_1 RGCY 7 cut(s) 62, 139, 220, 470, 482, 517, 526
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 1 cut(s) 272
DpnI GATC 6 cut(s) 362, 374, 486, 491, 509, 513
DpnII GATC 6 cut(s) 360, 372, 484, 489, 507, 511
EciI GGCGGA 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 516
EcoRII CCWGG 1 cut(s) 246
FaeI CATG 2 cut(s) 31, 58
FaiI YATR 3 cut(s) 29, 56, 434
FatI CATG 2 cut(s) 27, 54
Fnu4HI GCNGC 3 cut(s) 120, 218, 221
Fsp4HI GCNGC 3 cut(s) 120, 218, 221
GlaI GCGC 2 cut(s) 252, 402
GluI GCNGC 3 cut(s) 120, 218, 221
HaeIII GGCC 1 cut(s) 482
HapII CCGG 5 cut(s) 394, 413, 462, 473, 523
HgaI GACGC 2 cut(s) 203, 418
HhaI GCGC 2 cut(s) 253, 403
Hin1I GRCGYC 3 cut(s) 195, 288, 410
Hin1II CATG 2 cut(s) 31, 58
Hin6I GCGC 2 cut(s) 251, 401
HinP1I GCGC 2 cut(s) 251, 401
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HinfI GANTC 6 cut(s) 70, 86, 155, 175, 353, 388
HpaII CCGG 5 cut(s) 394, 413, 462, 473, 523
HphI GGTGA 3 cut(s) 170, 308, 528
Hpy166II GTNNAC 2 cut(s) 244, 408
Hpy188I TCNGA 6 cut(s) 91, 160, 352, 377, 489, 530
Hpy188III TCNNGA 2 cut(s) 364, 473
Hpy8I GTNNAC 2 cut(s) 244, 408
HpyCH4III ACNGT 1 cut(s) 534
HpyCH4IV ACGT 1 cut(s) 288
HpyF10VI GCNNNNNNNGC 4 cut(s) 312, 409, 467, 523
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 3 cut(s) 195, 288, 410
Hsp92II CATG 2 cut(s) 31, 58
HspAI GCGC 2 cut(s) 251, 401
Kpn2I TCCGGA 1 cut(s) 472
KroI GCCGGC 2 cut(s) 393, 522
KroNI GCCGGC 2 cut(s) 395, 524
Kzo9I GATC 6 cut(s) 360, 372, 484, 489, 507, 511
LmnI GCTCC 3 cut(s) 228, 475, 531
Lsp1109I GCAGC 2 cut(s) 207, 229
LweI GCATC 1 cut(s) 106
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 1 cut(s) 534
MalI GATC 6 cut(s) 362, 374, 486, 491, 509, 513
MboI GATC 6 cut(s) 360, 372, 484, 489, 507, 511
MboII GAAGA 8 cut(s) 173, 329, 332, 335, 338, 341, 392, 509
MflI RGATCY 1 cut(s) 489
MlyI GAGTC 1 cut(s) 397
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 7 cut(s) 49, 163, 249, 286, 360, 420, 430
MroI TCCGGA 1 cut(s) 472
MroNI GCCGGC 2 cut(s) 393, 522
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 2 cut(s) 278, 547
MspA1I CMGCKG 2 cut(s) 78, 220
MspI CCGG 5 cut(s) 394, 413, 462, 473, 523
MspR9I CCNGG 1 cut(s) 248
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 4 cut(s) 312, 409, 467, 523
NaeI GCCGGC 2 cut(s) 395, 524
NdeII GATC 6 cut(s) 360, 372, 484, 489, 507, 511
NgoMIV GCCGGC 2 cut(s) 393, 522
NlaIII CATG 2 cut(s) 31, 58
NlaIV GGNNCC 3 cut(s) 471, 491, 527
NmuCI GTSAC 1 cut(s) 534
PcsI WCGNNNNNNNCGW 6 cut(s) 108, 111, 120, 288, 362, 526
PdiI GCCGGC 2 cut(s) 395, 524
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 5 cut(s) 70, 86, 155, 175, 353
PflFI GACNNNGTC 1 cut(s) 287
PkrI GCNGC 3 cut(s) 121, 219, 222
Ple19I CGATCG 2 cut(s) 487, 514
PleI GAGTC 1 cut(s) 396
PpsI GAGTC 1 cut(s) 396
PshAI GACNNNNGTC 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 471, 491, 527
PspPI GGNCC 1 cut(s) 480
PsuI RGATCY 1 cut(s) 489
PsyI GACNNNGTC 1 cut(s) 287
PvuI CGATCG 2 cut(s) 487, 514
PvuII CAGCTG 1 cut(s) 220
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SaqAI TTAA 2 cut(s) 278, 547
SatI GCNGC 3 cut(s) 120, 218, 221
Sau3AI GATC 6 cut(s) 360, 372, 484, 489, 507, 511
Sau96I GGNCC 1 cut(s) 480
ScaI AGTACT 1 cut(s) 131
SchI GAGTC 1 cut(s) 397
ScrFI CCNGG 1 cut(s) 248
SetI ASST 3 cut(s) 41, 222, 291
SfaNI GCATC 1 cut(s) 106
SsiI CCGC 3 cut(s) 78, 120, 147
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 534
TaiI ACGT 1 cut(s) 291
TaqI TCGA 4 cut(s) 102, 153, 291, 510
TatI WGTACW 1 cut(s) 129
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 5 cut(s) 70, 86, 155, 175, 353
Tru1I TTAA 2 cut(s) 278, 547
Tru9I TTAA 2 cut(s) 278, 547
TseFI GTSAC 1 cut(s) 534
TseI GCWGC 2 cut(s) 217, 220
Tsp45I GTSAC 1 cut(s) 534
TspDTI ATGAA 2 cut(s) 43, 44
Tth111I GACNNNGTC 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 85
ZraI GACGTC 1 cut(s) 289
ZrmI AGTACT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.