RchiOBHm_Chr5g0071561

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013020
Physical Location & Seq
Forward (+)
77346363 .. 77347162
800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34662

Sequence Viewer

Length: 519 bp
ATGAACGCTACCTCTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAGCGGAAGCGATTCCGACACCAACATCGACGACGACGACGATGCCGCCGACGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGCAGCGAAGAAGAAGAAGATGTGATTGGAGTGGGTTCTGATTCGGCGATCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGACTGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCCGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

18.95

Weight (kDa)

4.16

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 34 - 165 7e-08 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccII CGCG 1 cut(s) 371
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 3 cut(s) 51, 93, 120
AclWI GGATC 3 cut(s) 454, 467, 485
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 3 cut(s) 168, 261, 380
AfaI GTAC 1 cut(s) 104
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 121, 380
AlwI GGATC 3 cut(s) 454, 467, 485
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 1 cut(s) 450
ApeKI GCWGC 3 cut(s) 190, 193, 285
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 226, 373
AspS9I GGNCC 1 cut(s) 450
AsuHPI GGTGA 3 cut(s) 143, 281, 498
BamHI GGATCC 1 cut(s) 459
BauI CACGAG 1 cut(s) 334
BbsI GAAGAC 2 cut(s) 141, 357
BbvI GCAGC 3 cut(s) 180, 202, 297
BccI CCATC 1 cut(s) 162
BceAI ACGGC 5 cut(s) 20, 107, 155, 220, 358
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 121, 380
BglI GCCNNNNNGGC 1 cut(s) 437
BisI GCNGC 4 cut(s) 93, 191, 194, 286
BlsI GCNGC 4 cut(s) 94, 192, 195, 287
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 3 cut(s) 441, 461, 497
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 79
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 2 cut(s) 141, 357
Bsa29I ATCGAT 1 cut(s) 480
BsaHI GRCGYC 3 cut(s) 168, 261, 380
BsaWI WCCGGW 1 cut(s) 442
Bse118I RCCGGY 2 cut(s) 363, 492
Bse1I ACTGG 2 cut(s) 113, 479
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 480
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 2 cut(s) 113, 479
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 3 cut(s) 180, 202, 297
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 2 cut(s) 457, 484
BshFI GGCC 1 cut(s) 452
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 2 cut(s) 457, 484
BsiSI CCGG 5 cut(s) 364, 383, 432, 443, 493
BsmAI GTCTC 2 cut(s) 121, 380
BsnI GGCC 1 cut(s) 452
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 6 cut(s) 330, 342, 454, 459, 477, 481
BspACI CCGC 3 cut(s) 51, 93, 120
BspANI GGCC 1 cut(s) 452
BspCNI CTCAG 1 cut(s) 237
BspDI ATCGAT 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 3 cut(s) 441, 461, 497
BspPI GGATC 3 cut(s) 454, 467, 485
BsrFI RCCGGY 2 cut(s) 363, 492
BsrI ACTGG 2 cut(s) 113, 479
BssAI RCCGGY 2 cut(s) 363, 492
BssMI GATC 6 cut(s) 330, 342, 454, 459, 477, 481
BssNI GRCGYC 3 cut(s) 168, 261, 380
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 504
BstACI GRCGYC 3 cut(s) 168, 261, 380
BstC8I GCNNGC 3 cut(s) 365, 369, 494
BstDEI CTNAG 1 cut(s) 245
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 2 cut(s) 226, 373
BstKTI GATC 6 cut(s) 333, 345, 457, 462, 480, 484
BstMAI GTCTC 2 cut(s) 121, 380
BstMBI GATC 6 cut(s) 330, 342, 454, 459, 477, 481
BstMCI CGRYCG 2 cut(s) 457, 484
BstMWI GCNNNNNNNGC 4 cut(s) 285, 379, 437, 493
BstNI CCWGG 1 cut(s) 221
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 219
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 180, 202, 297
BstV2I GAAGAC 2 cut(s) 141, 357
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
Bsu15I ATCGAT 1 cut(s) 480
BsuRI GGCC 1 cut(s) 452
BsuTUI ATCGAT 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 365, 369, 494
CfoI GCGC 2 cut(s) 226, 373
Cfr10I RCCGGY 2 cut(s) 363, 492
Cfr13I GGNCC 1 cut(s) 450
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 2 cut(s) 176, 388
Csp6I GTAC 1 cut(s) 103
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 35, 112, 193, 440, 452, 487, 496
CviKI_1 RGCY 7 cut(s) 35, 112, 193, 440, 452, 487, 496
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 1 cut(s) 245
DpnI GATC 6 cut(s) 332, 344, 456, 461, 479, 483
DpnII GATC 6 cut(s) 330, 342, 454, 459, 477, 481
EciI GGCGGA 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 404
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 4 cut(s) 93, 191, 194, 286
Fsp4HI GCNGC 4 cut(s) 93, 191, 194, 286
GlaI GCGC 2 cut(s) 225, 372
GluI GCNGC 4 cut(s) 93, 191, 194, 286
HaeIII GGCC 1 cut(s) 452
HapII CCGG 5 cut(s) 364, 383, 432, 443, 493
HgaI GACGC 2 cut(s) 176, 388
HhaI GCGC 2 cut(s) 226, 373
Hin1I GRCGYC 3 cut(s) 168, 261, 380
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 224, 371
HinP1I GCGC 2 cut(s) 224, 371
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 6 cut(s) 43, 59, 128, 148, 323, 358
HpaII CCGG 5 cut(s) 364, 383, 432, 443, 493
HphI GGTGA 3 cut(s) 143, 281, 498
Hpy166II GTNNAC 2 cut(s) 217, 378
Hpy188I TCNGA 6 cut(s) 64, 133, 322, 347, 459, 500
Hpy188III TCNNGA 2 cut(s) 334, 443
Hpy8I GTNNAC 2 cut(s) 217, 378
HpyCH4III ACNGT 1 cut(s) 504
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 379, 437, 493
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 3 cut(s) 168, 261, 380
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 224, 371
Kpn2I TCCGGA 1 cut(s) 442
KroI GCCGGC 2 cut(s) 363, 492
KroNI GCCGGC 2 cut(s) 365, 494
Kzo9I GATC 6 cut(s) 330, 342, 454, 459, 477, 481
LmnI GCTCC 3 cut(s) 201, 445, 501
Lsp1109I GCAGC 3 cut(s) 180, 202, 297
LweI GCATC 1 cut(s) 79
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 504
MalI GATC 6 cut(s) 332, 344, 456, 461, 479, 483
MboI GATC 6 cut(s) 330, 342, 454, 459, 477, 481
MboII GAAGA 7 cut(s) 146, 302, 305, 308, 311, 362, 479
MflI RGATCY 1 cut(s) 459
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 22, 136, 222, 259, 330, 390, 400, 410
MroI TCCGGA 1 cut(s) 442
MroNI GCCGGC 2 cut(s) 363, 492
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 2 cut(s) 251, 517
MspA1I CMGCKG 2 cut(s) 51, 193
MspI CCGG 5 cut(s) 364, 383, 432, 443, 493
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 285, 379, 437, 493
NaeI GCCGGC 2 cut(s) 365, 494
NdeII GATC 6 cut(s) 330, 342, 454, 459, 477, 481
NgoMIV GCCGGC 2 cut(s) 363, 492
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 3 cut(s) 441, 461, 497
NmuCI GTSAC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 6 cut(s) 81, 84, 93, 261, 332, 496
PdiI GCCGGC 2 cut(s) 365, 494
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 5 cut(s) 43, 59, 128, 148, 323
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 4 cut(s) 94, 192, 195, 287
Ple19I CGATCG 2 cut(s) 457, 484
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PshAI GACNNNNGTC 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 3 cut(s) 441, 461, 497
PspPI GGNCC 1 cut(s) 450
PsuI RGATCY 1 cut(s) 459
PsyI GACNNNGTC 1 cut(s) 260
PvuI CGATCG 2 cut(s) 457, 484
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 251, 517
SatI GCNGC 4 cut(s) 93, 191, 194, 286
Sau3AI GATC 6 cut(s) 330, 342, 454, 459, 477, 481
Sau96I GGNCC 1 cut(s) 450
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 1 cut(s) 221
SetI ASST 3 cut(s) 14, 195, 264
SfaNI GCATC 1 cut(s) 79
SsiI CCGC 3 cut(s) 51, 93, 120
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 504
TaiI ACGT 1 cut(s) 264
TaqI TCGA 4 cut(s) 75, 126, 264, 480
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 5 cut(s) 43, 59, 128, 148, 323
Tru1I TTAA 2 cut(s) 251, 517
Tru9I TTAA 2 cut(s) 251, 517
TseFI GTSAC 1 cut(s) 504
TseI GCWGC 3 cut(s) 190, 193, 285
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 2 cut(s) 16, 17
Tth111I GACNNNGTC 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 58
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.