RLG00000036225

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013020
Physical Location & Seq
Forward (+)
77624290 .. 77624900
611 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036225

Sequence Viewer

Length: 519 bp
ATGAACGCTACCTCTCCTAACGATTTCATGGAAGCTGTAGAAAACCCCAGCGGAAGCGATTCCGACACCAACATCGACGACGACGATGCCTCCGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGATGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTCATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTCAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGCAGCGAAGAAGAAGAAGAAGAAGAAGATCTGATTGGAGAGGGTTCTGATTCGGCGATCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGAATGCGACCAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCGGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGGCAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.04

Weight (kDa)

4.1

Isoelectric Point (pI)

67.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 32 - 165 2.2e-07 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 255
AccII CGCG 1 cut(s) 371
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 3 cut(s) 51, 114, 452
AclWI GGATC 4 cut(s) 454, 462, 467, 485
AcuI CTGAAG 1 cut(s) 486
AcyI GRCGYC 4 cut(s) 162, 198, 252, 380
AfaI GTAC 1 cut(s) 98
AjnI CCWGG 2 cut(s) 210, 395
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 2 cut(s) 35, 184
AluI AGCT 2 cut(s) 35, 184
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 4 cut(s) 454, 462, 467, 485
Aor13HI TCCGGA 1 cut(s) 442
ApeKI GCWGC 3 cut(s) 181, 184, 276
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 217, 373
AsuHPI GGTGA 3 cut(s) 137, 272, 498
BamHI GGATCC 1 cut(s) 459
BauI CACGAG 1 cut(s) 334
BbsI GAAGAC 1 cut(s) 357
BbvI GCAGC 3 cut(s) 171, 193, 288
BccI CCATC 1 cut(s) 156
BceAI ACGGC 4 cut(s) 101, 149, 211, 358
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 2 cut(s) 212, 397
BcoDI GTCTC 1 cut(s) 115
BfmI CTRYAG 1 cut(s) 36
BglI GCCNNNNNGGC 1 cut(s) 437
BglII AGATCT 1 cut(s) 301
BisI GCNGC 3 cut(s) 182, 185, 277
BlsI GCNGC 3 cut(s) 183, 186, 278
BmcAI AGTACT 1 cut(s) 98
Bme1390I CCNGG 2 cut(s) 212, 397
BmiI GGNNCC 3 cut(s) 441, 461, 497
BmrFI CCNGG 2 cut(s) 212, 397
BmsI GCATC 1 cut(s) 76
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 1 cut(s) 357
Bsa29I ATCGAT 1 cut(s) 480
BsaHI GRCGYC 4 cut(s) 162, 198, 252, 380
BsaJI CCNNGG 1 cut(s) 396
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse118I RCCGGY 2 cut(s) 363, 492
Bse1I ACTGG 2 cut(s) 107, 479
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 2 cut(s) 212, 397
BseCI ATCGAT 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 107, 479
BseRI GAGGAG 1 cut(s) 152
BseXI GCAGC 3 cut(s) 171, 193, 288
BseYI CCCAGC 1 cut(s) 47
Bsh1236I CGCG 1 cut(s) 371
Bsh1285I CGRYCG 1 cut(s) 484
BshVI ATCGAT 1 cut(s) 480
BsiEI CGRYCG 1 cut(s) 484
BsiSI CCGG 6 cut(s) 364, 383, 432, 443, 493, 499
BsmAI GTCTC 1 cut(s) 115
BsmI GAATGC 1 cut(s) 394
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
BspACI CCGC 3 cut(s) 51, 114, 452
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 480
BspEI TCCGGA 1 cut(s) 442
BspFNI CGCG 1 cut(s) 371
BspLI GGNNCC 3 cut(s) 441, 461, 497
BspPI GGATC 4 cut(s) 454, 462, 467, 485
BsrFI RCCGGY 2 cut(s) 363, 492
BsrI ACTGG 2 cut(s) 107, 479
BssAI RCCGGY 2 cut(s) 363, 492
BssECI CCNNGG 1 cut(s) 396
BssMI GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
BssNI GRCGYC 4 cut(s) 162, 198, 252, 380
BssSI CACGAG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 334
Bst2UI CCWGG 2 cut(s) 212, 397
BstACI GRCGYC 4 cut(s) 162, 198, 252, 380
BstC8I GCNNGC 3 cut(s) 365, 369, 494
BstDEI CTNAG 1 cut(s) 236
BstFNI CGCG 1 cut(s) 371
BstHHI GCGC 2 cut(s) 217, 373
BstKTI GATC 7 cut(s) 304, 333, 345, 457, 462, 480, 484
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
BstMCI CGRYCG 1 cut(s) 484
BstMWI GCNNNNNNNGC 4 cut(s) 276, 379, 437, 493
BstNI CCWGG 2 cut(s) 212, 397
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 210, 395
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 1 cut(s) 371
BstV1I GCAGC 3 cut(s) 171, 193, 288
BstV2I GAAGAC 1 cut(s) 357
BstX2I RGATCY 2 cut(s) 301, 459
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 2 cut(s) 301, 459
Bsu15I ATCGAT 1 cut(s) 480
BsuTUI ATCGAT 1 cut(s) 480
BtgZI GCGATG 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 365, 369, 494
CfoI GCGC 2 cut(s) 217, 373
Cfr10I RCCGGY 2 cut(s) 363, 492
ClaI ATCGAT 1 cut(s) 480
CseI GACGC 3 cut(s) 170, 187, 388
Csp6I GTAC 1 cut(s) 97
CviAII CATG 1 cut(s) 28
CviJI RGCY 6 cut(s) 35, 106, 184, 440, 487, 496
CviKI_1 RGCY 6 cut(s) 35, 106, 184, 440, 487, 496
CviQI GTAC 1 cut(s) 97
DdeI CTNAG 1 cut(s) 236
DpnI GATC 7 cut(s) 303, 332, 344, 456, 461, 479, 483
DpnII GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
EciI GGCGGA 2 cut(s) 103, 467
Eco57I CTGAAG 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 210, 395
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 404
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 3 cut(s) 182, 185, 277
Fsp4HI GCNGC 3 cut(s) 182, 185, 277
GlaI GCGC 2 cut(s) 216, 372
GluI GCNGC 3 cut(s) 182, 185, 277
GsaI CCCAGC 1 cut(s) 51
HapII CCGG 6 cut(s) 364, 383, 432, 443, 493, 499
HgaI GACGC 3 cut(s) 170, 187, 388
HhaI GCGC 2 cut(s) 217, 373
Hin1I GRCGYC 4 cut(s) 162, 198, 252, 380
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 215, 371
HinP1I GCGC 2 cut(s) 215, 371
HincII GTYRAC 1 cut(s) 378
HindII GTYRAC 1 cut(s) 378
HinfI GANTC 5 cut(s) 59, 122, 142, 323, 358
HpaII CCGG 6 cut(s) 364, 383, 432, 443, 493, 499
HphI GGTGA 3 cut(s) 137, 272, 498
Hpy166II GTNNAC 2 cut(s) 208, 378
Hpy188I TCNGA 8 cut(s) 64, 94, 127, 239, 306, 322, 347, 459
Hpy188III TCNNGA 2 cut(s) 334, 443
Hpy8I GTNNAC 2 cut(s) 208, 378
Hpy99I CGWCG 9 cut(s) 80, 83, 86, 122, 164, 235, 251, 254, 257
HpyCH4IV ACGT 1 cut(s) 252
HpyF10VI GCNNNNNNNGC 4 cut(s) 276, 379, 437, 493
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 4 cut(s) 162, 198, 252, 380
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 215, 371
Kpn2I TCCGGA 1 cut(s) 442
KroI GCCGGC 2 cut(s) 363, 492
KroNI GCCGGC 2 cut(s) 365, 494
Kzo9I GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
LmnI GCTCC 3 cut(s) 192, 445, 501
Lsp1109I GCAGC 3 cut(s) 171, 193, 288
LweI GCATC 1 cut(s) 76
MaeII ACGT 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 504
MalI GATC 7 cut(s) 303, 332, 344, 456, 461, 479, 483
MboI GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
MflI RGATCY 2 cut(s) 301, 459
MlyI GAGTC 1 cut(s) 367
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 22, 100, 130, 213, 250, 307, 330, 400, 410
MroI TCCGGA 1 cut(s) 442
MroNI GCCGGC 2 cut(s) 363, 492
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 2 cut(s) 242, 517
MspA1I CMGCKG 2 cut(s) 51, 184
MspI CCGG 6 cut(s) 364, 383, 432, 443, 493, 499
MspR9I CCNGG 2 cut(s) 212, 397
Mva1269I GAATGC 1 cut(s) 394
MvaI CCWGG 2 cut(s) 212, 397
MvnI CGCG 1 cut(s) 371
MwoI GCNNNNNNNGC 4 cut(s) 276, 379, 437, 493
NaeI GCCGGC 2 cut(s) 365, 494
NdeII GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
NgoMIV GCCGGC 2 cut(s) 363, 492
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 3 cut(s) 441, 461, 497
NmuCI GTSAC 1 cut(s) 504
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 90, 252, 332
PctI GAATGC 1 cut(s) 394
PdiI GCCGGC 2 cut(s) 365, 494
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 4 cut(s) 59, 122, 142, 323
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 3 cut(s) 183, 186, 278
Ple19I CGATCG 1 cut(s) 484
PleI GAGTC 1 cut(s) 366
PpsI GAGTC 1 cut(s) 366
PshAI GACNNNNGTC 1 cut(s) 357
Psp6I CCWGG 2 cut(s) 210, 395
PspFI CCCAGC 1 cut(s) 47
PspGI CCWGG 2 cut(s) 210, 395
PspN4I GGNNCC 3 cut(s) 441, 461, 497
PsuI RGATCY 2 cut(s) 301, 459
PsyI GACNNNGTC 1 cut(s) 251
PvuI CGATCG 1 cut(s) 484
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 98
RsaNI GTAC 1 cut(s) 97
SaqAI TTAA 2 cut(s) 242, 517
SatI GCNGC 3 cut(s) 182, 185, 277
Sau3AI GATC 7 cut(s) 301, 330, 342, 454, 459, 477, 481
ScaI AGTACT 1 cut(s) 98
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 212, 397
SetI ASST 4 cut(s) 14, 37, 186, 255
SfaNI GCATC 1 cut(s) 76
SfcI CTRYAG 1 cut(s) 36
SsiI CCGC 3 cut(s) 51, 114, 452
StyD4I CCNGG 2 cut(s) 210, 395
TaiI ACGT 1 cut(s) 255
TaqI TCGA 4 cut(s) 75, 120, 255, 480
TatI WGTACW 1 cut(s) 96
TfiI GAWTC 4 cut(s) 59, 122, 142, 323
Tru1I TTAA 2 cut(s) 242, 517
Tru9I TTAA 2 cut(s) 242, 517
TseFI GTSAC 1 cut(s) 504
TseI GCWGC 3 cut(s) 181, 184, 276
Tsp45I GTSAC 1 cut(s) 504
TspDTI ATGAA 2 cut(s) 16, 17
Tth111I GACNNNGTC 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 58
ZraI GACGTC 1 cut(s) 253
ZrmI AGTACT 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.