Rh5BG488300

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0013020
Physical Location & Seq
Forward (+)
77802734 .. 77803724
991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG488300.1

Sequence Viewer

Length: 522 bp
ATGAACGCTACCTCTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAGCGGAAGCGATTCCGACACCAACATCGACGACGACGACGATGCCGCCGACGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTGGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAATGGCAGTAGAAGCGAAGAAGAAGAAGAAGATGTGATTGGAGTGGGTTCTGATTCGGCGATCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGACTGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCCGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

173

Amino Acids

19.15

Weight (kDa)

4.19

Isoelectric Point (pI)

62.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 34 - 166 2.3e-07 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 264
AccII CGCG 1 cut(s) 374
AccIII TCCGGA 1 cut(s) 445
AciI CCGC 3 cut(s) 51, 93, 120
AclWI GGATC 3 cut(s) 457, 470, 488
AcuI CTGAAG 1 cut(s) 489
AcyI GRCGYC 3 cut(s) 168, 261, 383
AfaI GTAC 1 cut(s) 104
AjnI CCWGG 1 cut(s) 219
AjuI GAANNNNNNNTTGG 2 cut(s) 294, 326
AluBI AGCT 1 cut(s) 193
AluI AGCT 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 121, 383
AlwI GGATC 3 cut(s) 457, 470, 488
Aor13HI TCCGGA 1 cut(s) 445
AoxI GGCC 1 cut(s) 453
ApeKI GCWGC 2 cut(s) 190, 193
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 226, 376
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 3 cut(s) 143, 281, 501
BamHI GGATCC 1 cut(s) 462
BauI CACGAG 1 cut(s) 337
BbsI GAAGAC 2 cut(s) 141, 360
BbvI GCAGC 2 cut(s) 180, 202
BccI CCATC 1 cut(s) 162
BceAI ACGGC 5 cut(s) 20, 107, 155, 220, 361
BcgI CGANNNNNNTGC 2 cut(s) 71, 105
BciT130I CCWGG 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 121, 383
BglI GCCNNNNNGGC 1 cut(s) 440
BisI GCNGC 3 cut(s) 93, 191, 194
BlsI GCNGC 3 cut(s) 94, 192, 195
BmcAI AGTACT 1 cut(s) 104
Bme1390I CCNGG 1 cut(s) 221
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 3 cut(s) 444, 464, 500
BmrFI CCNGG 1 cut(s) 221
BmsI GCATC 1 cut(s) 79
BoxI GACNNNNGTC 1 cut(s) 360
BpiI GAAGAC 2 cut(s) 141, 360
Bsa29I ATCGAT 1 cut(s) 483
BsaHI GRCGYC 3 cut(s) 168, 261, 383
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 445
Bse118I RCCGGY 2 cut(s) 366, 495
Bse1I ACTGG 2 cut(s) 113, 482
BseAI TCCGGA 1 cut(s) 445
BseBI CCWGG 1 cut(s) 221
BseCI ATCGAT 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 236
BseNI ACTGG 2 cut(s) 113, 482
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 2 cut(s) 180, 202
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 2 cut(s) 460, 487
BshFI GGCC 1 cut(s) 455
BshVI ATCGAT 1 cut(s) 483
BsiEI CGRYCG 2 cut(s) 460, 487
BsiSI CCGG 5 cut(s) 367, 386, 435, 446, 496
BsmAI GTCTC 2 cut(s) 121, 383
BsnI GGCC 1 cut(s) 455
Bsp13I TCCGGA 1 cut(s) 445
Bsp143I GATC 6 cut(s) 333, 345, 457, 462, 480, 484
BspACI CCGC 3 cut(s) 51, 93, 120
BspANI GGCC 1 cut(s) 455
BspCNI CTCAG 1 cut(s) 237
BspDI ATCGAT 1 cut(s) 483
BspEI TCCGGA 1 cut(s) 445
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 3 cut(s) 444, 464, 500
BspPI GGATC 3 cut(s) 457, 470, 488
BsrFI RCCGGY 2 cut(s) 366, 495
BsrI ACTGG 2 cut(s) 113, 482
BssAI RCCGGY 2 cut(s) 366, 495
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 6 cut(s) 333, 345, 457, 462, 480, 484
BssNI GRCGYC 3 cut(s) 168, 261, 383
BssSI CACGAG 1 cut(s) 337
Bst2BI CACGAG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 221
Bst4CI ACNGT 1 cut(s) 507
BstACI GRCGYC 3 cut(s) 168, 261, 383
BstC8I GCNNGC 3 cut(s) 368, 372, 497
BstDEI CTNAG 1 cut(s) 245
BstDSI CCRYGG 1 cut(s) 171
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 2 cut(s) 226, 376
BstKTI GATC 6 cut(s) 336, 348, 460, 465, 483, 487
BstMAI GTCTC 2 cut(s) 121, 383
BstMBI GATC 6 cut(s) 333, 345, 457, 462, 480, 484
BstMCI CGRYCG 2 cut(s) 460, 487
BstMWI GCNNNNNNNGC 4 cut(s) 285, 382, 440, 496
BstNI CCWGG 1 cut(s) 221
BstPAI GACNNNNGTC 1 cut(s) 360
BstSCI CCNGG 1 cut(s) 219
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 2 cut(s) 180, 202
BstV2I GAAGAC 2 cut(s) 141, 360
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
Bsu15I ATCGAT 1 cut(s) 483
BsuRI GGCC 1 cut(s) 455
BsuTUI ATCGAT 1 cut(s) 483
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 428
Cac8I GCNNGC 3 cut(s) 368, 372, 497
CfoI GCGC 2 cut(s) 226, 376
Cfr10I RCCGGY 2 cut(s) 366, 495
Cfr13I GGNCC 1 cut(s) 453
ClaI ATCGAT 1 cut(s) 483
CseI GACGC 2 cut(s) 176, 391
Csp6I GTAC 1 cut(s) 103
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 35, 112, 193, 443, 455, 490, 499
CviKI_1 RGCY 7 cut(s) 35, 112, 193, 443, 455, 490, 499
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 1 cut(s) 245
DpnI GATC 6 cut(s) 335, 347, 459, 464, 482, 486
DpnII GATC 6 cut(s) 333, 345, 457, 462, 480, 484
EciI GGCGGA 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 489
EcoRII CCWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 407
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 3 cut(s) 93, 191, 194
Fsp4HI GCNGC 3 cut(s) 93, 191, 194
GlaI GCGC 2 cut(s) 225, 375
GluI GCNGC 3 cut(s) 93, 191, 194
HaeIII GGCC 1 cut(s) 455
HapII CCGG 5 cut(s) 367, 386, 435, 446, 496
HgaI GACGC 2 cut(s) 176, 391
HhaI GCGC 2 cut(s) 226, 376
Hin1I GRCGYC 3 cut(s) 168, 261, 383
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 224, 374
HinP1I GCGC 2 cut(s) 224, 374
HincII GTYRAC 1 cut(s) 381
HindII GTYRAC 1 cut(s) 381
HinfI GANTC 6 cut(s) 43, 59, 128, 148, 326, 361
HpaII CCGG 5 cut(s) 367, 386, 435, 446, 496
HphI GGTGA 3 cut(s) 143, 281, 501
Hpy166II GTNNAC 2 cut(s) 217, 381
Hpy188I TCNGA 6 cut(s) 64, 133, 325, 350, 462, 503
Hpy188III TCNNGA 2 cut(s) 337, 446
Hpy8I GTNNAC 2 cut(s) 217, 381
HpyCH4III ACNGT 1 cut(s) 507
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 4 cut(s) 285, 382, 440, 496
HpyF3I CTNAG 1 cut(s) 245
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 3 cut(s) 168, 261, 383
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 224, 374
Kpn2I TCCGGA 1 cut(s) 445
KroI GCCGGC 2 cut(s) 366, 495
KroNI GCCGGC 2 cut(s) 368, 497
Kzo9I GATC 6 cut(s) 333, 345, 457, 462, 480, 484
LmnI GCTCC 3 cut(s) 201, 448, 504
Lsp1109I GCAGC 2 cut(s) 180, 202
LweI GCATC 1 cut(s) 79
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 507
MalI GATC 6 cut(s) 335, 347, 459, 464, 482, 486
MboI GATC 6 cut(s) 333, 345, 457, 462, 480, 484
MboII GAAGA 8 cut(s) 146, 302, 305, 308, 311, 314, 365, 482
MflI RGATCY 1 cut(s) 462
MlyI GAGTC 1 cut(s) 370
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 22, 136, 222, 259, 333, 393, 403, 413
MroI TCCGGA 1 cut(s) 445
MroNI GCCGGC 2 cut(s) 366, 495
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 2 cut(s) 251, 520
MspA1I CMGCKG 2 cut(s) 51, 193
MspI CCGG 5 cut(s) 367, 386, 435, 446, 496
MspR9I CCNGG 1 cut(s) 221
MvaI CCWGG 1 cut(s) 221
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 4 cut(s) 285, 382, 440, 496
NaeI GCCGGC 2 cut(s) 368, 497
NdeII GATC 6 cut(s) 333, 345, 457, 462, 480, 484
NgoMIV GCCGGC 2 cut(s) 366, 495
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 3 cut(s) 444, 464, 500
NmuCI GTSAC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 6 cut(s) 81, 84, 93, 261, 335, 499
PdiI GCCGGC 2 cut(s) 368, 497
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 5 cut(s) 43, 59, 128, 148, 326
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 94, 192, 195
Ple19I CGATCG 2 cut(s) 460, 487
PleI GAGTC 1 cut(s) 369
PpsI GAGTC 1 cut(s) 369
PshAI GACNNNNGTC 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 219
PspGI CCWGG 1 cut(s) 219
PspN4I GGNNCC 3 cut(s) 444, 464, 500
PspPI GGNCC 1 cut(s) 453
PsuI RGATCY 1 cut(s) 462
PsyI GACNNNGTC 1 cut(s) 260
PvuI CGATCG 2 cut(s) 460, 487
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SaqAI TTAA 2 cut(s) 251, 520
SatI GCNGC 3 cut(s) 93, 191, 194
Sau3AI GATC 6 cut(s) 333, 345, 457, 462, 480, 484
Sau96I GGNCC 1 cut(s) 453
ScaI AGTACT 1 cut(s) 104
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 1 cut(s) 221
SetI ASST 3 cut(s) 14, 195, 264
SfaNI GCATC 1 cut(s) 79
SsiI CCGC 3 cut(s) 51, 93, 120
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 507
TaiI ACGT 1 cut(s) 264
TaqI TCGA 4 cut(s) 75, 126, 264, 483
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 95
TfiI GAWTC 5 cut(s) 43, 59, 128, 148, 326
Tru1I TTAA 2 cut(s) 251, 520
Tru9I TTAA 2 cut(s) 251, 520
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 2 cut(s) 190, 193
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 2 cut(s) 16, 17
Tth111I GACNNNGTC 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 58
ZraI GACGTC 1 cut(s) 262
ZrmI AGTACT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.