Rh5DG499100

iron import into the mitochondrion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013020
Physical Location & Seq
Forward (+)
78271990 .. 78272970
981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG499100.1

Sequence Viewer

Length: 513 bp
ATGAACGCTACCTTTCCTAACGATTTCATGGAAGCCGTAGAGAATCACAACGGAAGCGATTCCGACACCAACATCGACGACGACGATGCCGCCGCCGAGTACTACCAGCCAGTCTCCGCCGTCGATTCCGAAGACGACGAGGAGAATCACCATCTCCAAAACGACGCCGTTGTTTCCCACTCGCAGCTGCTCCCCAACGGCGTGATTGTGAACCAGGCGCAGAGGGGAGTGTCGTCGCTGAGATTAAACGACGACGTCGAGGGTGATAGTGGCAGTAGCAGCGAAGAAGAAGAAGATGTGATTGGAGGGGGTTCTGATTCGGCGGTCACGAGGGCGATCAGAGAAGACGAGAGTCGCCGGCACGCGCCGTTGACGCCGGAGAATGCGACGAGGGTTATGGAGGCGATGAGAGGGATTTCGTTCGCCGGAGTGGCTCCGGATTGGGCTGATCGGATCCCTGAAGAAAACTGGATCGATCGGCTTCGCCGGCTCCGACAGTCACCCCAGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

18.71

Weight (kDa)

4.21

Isoelectric Point (pI)

63.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MEA1 PF06910 33 - 163 1.7e-08 Male enhanced antigen 1 (MEA1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013020)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07020
fragaria_vesca FvH4_3g21494 FvH4_3g39700 FvH4_3g39700
malus_domestica MD03G1055700.v1.1
prunus_persica Prupe.6G049000_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0071561
rosa_laevigata RLG00000013433 RLG00000036225
rosa_multiflora Rmu_sc0000031.1_g000030 Rmu_sc0005745.1_g000008
rosa_roxburghii Rroxscaffold_1G00009610
rosa_rugosa Rorug05G0413200
rosa_samantha Rh5AG469100 Rh5BG488300 Rh5DG499100
rosa_wichuraiana Rw5G043590 Rw6G041020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccII CGCG 1 cut(s) 365
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 4 cut(s) 90, 93, 117, 323
AclWI GGATC 3 cut(s) 448, 461, 479
AcuI CTGAAG 1 cut(s) 480
AcyI GRCGYC 3 cut(s) 165, 255, 374
AfaI GTAC 1 cut(s) 101
AjnI CCWGG 1 cut(s) 213
AjuI GAANNNNNNNTTGG 2 cut(s) 285, 317
AluBI AGCT 1 cut(s) 187
AluI AGCT 1 cut(s) 187
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 3 cut(s) 448, 461, 479
Aor13HI TCCGGA 1 cut(s) 436
ApeKI GCWGC 3 cut(s) 184, 187, 279
Asp700I GAANNNNTTC 1 cut(s) 58
AspLEI GCGC 2 cut(s) 220, 367
AsuHPI GGTGA 3 cut(s) 140, 275, 492
BamHI GGATCC 1 cut(s) 453
BauI CACGAG 1 cut(s) 328
BbsI GAAGAC 2 cut(s) 138, 351
BbvI GCAGC 3 cut(s) 174, 196, 291
BccI CCATC 1 cut(s) 159
BceAI ACGGC 5 cut(s) 20, 104, 152, 214, 352
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 118
BglI GCCNNNNNGGC 1 cut(s) 431
BisI GCNGC 5 cut(s) 90, 93, 185, 188, 280
BlsI GCNGC 5 cut(s) 91, 94, 186, 189, 281
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 215
BmiI GGNNCC 3 cut(s) 435, 455, 491
BmrFI CCNGG 1 cut(s) 215
BmsI GCATC 1 cut(s) 76
BoxI GACNNNNGTC 1 cut(s) 351
BpiI GAAGAC 2 cut(s) 138, 351
Bsa29I ATCGAT 1 cut(s) 474
BsaHI GRCGYC 3 cut(s) 165, 255, 374
BsaWI WCCGGW 1 cut(s) 436
Bse118I RCCGGY 2 cut(s) 357, 486
Bse1I ACTGG 2 cut(s) 110, 473
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 474
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 2 cut(s) 110, 473
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 3 cut(s) 174, 196, 291
Bsh1236I CGCG 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 478
BshVI ATCGAT 1 cut(s) 474
BsiEI CGRYCG 1 cut(s) 478
BsiSI CCGG 5 cut(s) 358, 377, 426, 437, 487
BsmAI GTCTC 1 cut(s) 118
BsmI GAATGC 1 cut(s) 388
Bsp13I TCCGGA 1 cut(s) 436
Bsp143I GATC 5 cut(s) 336, 448, 453, 471, 475
BspACI CCGC 4 cut(s) 90, 93, 117, 323
BspCNI CTCAG 1 cut(s) 231
BspDI ATCGAT 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 1 cut(s) 365
BspLI GGNNCC 3 cut(s) 435, 455, 491
BspPI GGATC 3 cut(s) 448, 461, 479
BsrFI RCCGGY 2 cut(s) 357, 486
BsrI ACTGG 2 cut(s) 110, 473
BssAI RCCGGY 2 cut(s) 357, 486
BssMI GATC 5 cut(s) 336, 448, 453, 471, 475
BssNI GRCGYC 3 cut(s) 165, 255, 374
BssSI CACGAG 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 498
BstACI GRCGYC 3 cut(s) 165, 255, 374
BstC8I GCNNGC 3 cut(s) 359, 363, 488
BstDEI CTNAG 1 cut(s) 239
BstFNI CGCG 1 cut(s) 365
BstHHI GCGC 2 cut(s) 220, 367
BstKTI GATC 5 cut(s) 339, 451, 456, 474, 478
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 5 cut(s) 336, 448, 453, 471, 475
BstMCI CGRYCG 1 cut(s) 478
BstMWI GCNNNNNNNGC 4 cut(s) 279, 373, 431, 487
BstNI CCWGG 1 cut(s) 215
BstPAI GACNNNNGTC 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 213
BstUI CGCG 1 cut(s) 365
BstV1I GCAGC 3 cut(s) 174, 196, 291
BstV2I GAAGAC 2 cut(s) 138, 351
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
Bsu15I ATCGAT 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 419
Cac8I GCNNGC 3 cut(s) 359, 363, 488
CfoI GCGC 2 cut(s) 220, 367
Cfr10I RCCGGY 2 cut(s) 357, 486
ClaI ATCGAT 1 cut(s) 474
CseI GACGC 2 cut(s) 173, 382
Csp6I GTAC 1 cut(s) 100
CviAII CATG 1 cut(s) 28
CviJI RGCY 7 cut(s) 35, 109, 187, 434, 446, 481, 490
CviKI_1 RGCY 7 cut(s) 35, 109, 187, 434, 446, 481, 490
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 1 cut(s) 239
DpnI GATC 5 cut(s) 338, 450, 455, 473, 477
DpnII GATC 5 cut(s) 336, 448, 453, 471, 475
EciI GGCGGA 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 213
FaeI CATG 1 cut(s) 31
FaiI YATR 2 cut(s) 29, 398
FatI CATG 1 cut(s) 27
Fnu4HI GCNGC 5 cut(s) 90, 93, 185, 188, 280
Fsp4HI GCNGC 5 cut(s) 90, 93, 185, 188, 280
GlaI GCGC 2 cut(s) 219, 366
GluI GCNGC 5 cut(s) 90, 93, 185, 188, 280
HapII CCGG 5 cut(s) 358, 377, 426, 437, 487
HgaI GACGC 2 cut(s) 173, 382
HhaI GCGC 2 cut(s) 220, 367
Hin1I GRCGYC 3 cut(s) 165, 255, 374
Hin1II CATG 1 cut(s) 31
Hin6I GCGC 2 cut(s) 218, 365
HinP1I GCGC 2 cut(s) 218, 365
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 6 cut(s) 43, 59, 125, 145, 317, 352
HpaII CCGG 5 cut(s) 358, 377, 426, 437, 487
HphI GGTGA 3 cut(s) 140, 275, 492
Hpy166II GTNNAC 2 cut(s) 211, 372
Hpy188I TCNGA 6 cut(s) 64, 130, 316, 341, 453, 494
Hpy188III TCNNGA 2 cut(s) 328, 437
Hpy8I GTNNAC 2 cut(s) 211, 372
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4IV ACGT 1 cut(s) 255
HpyF10VI GCNNNNNNNGC 4 cut(s) 279, 373, 431, 487
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 255
Hsp92I GRCGYC 3 cut(s) 165, 255, 374
Hsp92II CATG 1 cut(s) 31
HspAI GCGC 2 cut(s) 218, 365
Kpn2I TCCGGA 1 cut(s) 436
KroI GCCGGC 2 cut(s) 357, 486
KroNI GCCGGC 2 cut(s) 359, 488
Kzo9I GATC 5 cut(s) 336, 448, 453, 471, 475
LmnI GCTCC 3 cut(s) 195, 439, 495
Lsp1109I GCAGC 3 cut(s) 174, 196, 291
LweI GCATC 1 cut(s) 76
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 2 cut(s) 325, 498
MalI GATC 5 cut(s) 338, 450, 455, 473, 477
MboI GATC 5 cut(s) 336, 448, 453, 471, 475
MboII GAAGA 7 cut(s) 143, 296, 299, 302, 305, 356, 473
MflI RGATCY 1 cut(s) 453
MlyI GAGTC 1 cut(s) 361
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 8 cut(s) 133, 216, 253, 299, 324, 384, 394, 404
MroI TCCGGA 1 cut(s) 436
MroNI GCCGGC 2 cut(s) 357, 486
MroXI GAANNNNTTC 1 cut(s) 58
MseI TTAA 2 cut(s) 245, 511
MspA1I CMGCKG 1 cut(s) 187
MspI CCGG 5 cut(s) 358, 377, 426, 437, 487
MspR9I CCNGG 1 cut(s) 215
Mva1269I GAATGC 1 cut(s) 388
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 365
MwoI GCNNNNNNNGC 4 cut(s) 279, 373, 431, 487
NaeI GCCGGC 2 cut(s) 359, 488
NdeII GATC 5 cut(s) 336, 448, 453, 471, 475
NgoMIV GCCGGC 2 cut(s) 357, 486
NlaIII CATG 1 cut(s) 31
NlaIV GGNNCC 3 cut(s) 435, 455, 491
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 2 cut(s) 325, 498
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 255, 326, 490
PctI GAATGC 1 cut(s) 388
PdiI GCCGGC 2 cut(s) 359, 488
PdmI GAANNNNTTC 1 cut(s) 58
PfeI GAWTC 5 cut(s) 43, 59, 125, 145, 317
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 5 cut(s) 91, 94, 186, 189, 281
Ple19I CGATCG 1 cut(s) 478
PleI GAGTC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 360
PshAI GACNNNNGTC 1 cut(s) 351
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 3 cut(s) 435, 455, 491
PsuI RGATCY 1 cut(s) 453
PsyI GACNNNGTC 1 cut(s) 254
PvuI CGATCG 1 cut(s) 478
PvuII CAGCTG 1 cut(s) 187
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 245, 511
SatI GCNGC 5 cut(s) 90, 93, 185, 188, 280
Sau3AI GATC 5 cut(s) 336, 448, 453, 471, 475
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 215
SetI ASST 3 cut(s) 14, 189, 258
SfaNI GCATC 1 cut(s) 76
SsiI CCGC 4 cut(s) 90, 93, 117, 323
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 1 cut(s) 498
TaiI ACGT 1 cut(s) 258
TaqI TCGA 4 cut(s) 75, 123, 258, 474
TatI WGTACW 1 cut(s) 99
TauI GCSGC 2 cut(s) 92, 95
TfiI GAWTC 5 cut(s) 43, 59, 125, 145, 317
Tru1I TTAA 2 cut(s) 245, 511
Tru9I TTAA 2 cut(s) 245, 511
TseFI GTSAC 2 cut(s) 325, 498
TseI GCWGC 3 cut(s) 184, 187, 279
Tsp45I GTSAC 2 cut(s) 325, 498
TspDTI ATGAA 2 cut(s) 16, 17
TspGWI ACGGA 1 cut(s) 66
Tth111I GACNNNGTC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 58
ZraI GACGTC 1 cut(s) 256
ZrmI AGTACT 1 cut(s) 101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.