MD03G1063100.v1.1

SAGA-associated factor 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007301
Physical Location & Seq
Forward (+)
5085602 .. 5087761
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1063100.v1.1.491

Sequence Viewer

Length: 546 bp
ATGTCGCTTCCAAATGAAGAAAATGAAATGTCCTCTTCTCCCGATGCTCAGCTTTCGTCTCATGTTTTTGGGGCCCTCCTCGATTCAATTATTGTTGATGTTGCATCTGAGTGTCATCGAATAGCAAAGTTGGGTCTTGATCGTAATTTTGAAGAAGAGGAAGAAGAACTAAGGCTTTCAGCCCAAGCACGGGTGAGGGTAGCTGATCCTGGTAATAGTGGTGAAGCAAATGGCAAGTACGTGGTTGACATATTTGGGCAGAATCATCCTTCTGTAGCCAGTGAAGTGTTTGAGTGCATGAATTGTGGTCGGTCTATCGTTGCTGGGAGGTTTGCTCCTCATCTGGAGAAGTGTATGGGAAAGGGTAGAAAGGCTCGCCTCAAAGTGACAAGAAGTAGCACGGCTGCACAGACTCGGAATTCACGGGGAAACTCTGCATCTACATACTCCACATATTCAAATTCCAACAGCACAAACCGATTATCAAATGGAGCATCCGCTGTTGCTGGTGAGGAGTACTCGATTGGTGCATTGGAAGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sgf11

Protein Analysis

182

Amino Acids

19.44

Weight (kDa)

5.44

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sgf11 PF08209 94 - 124 8.3e-17 Sgf11 (transcriptional regulation protein)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 498
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 2 cut(s) 418, 460
AfaI GTAC 2 cut(s) 239, 518
AfiI CCNNNNNNNGG 2 cut(s) 189, 190
AgsI TTSAA 3 cut(s) 87, 152, 459
AjnI CCWGG 1 cut(s) 208
AluBI AGCT 2 cut(s) 52, 203
AluI AGCT 2 cut(s) 52, 203
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 72
ApaI GGGCCC 1 cut(s) 76
ApeKI GCWGC 1 cut(s) 404
ApoI RAATTY 2 cut(s) 418, 460
AspS9I GGNCC 2 cut(s) 72, 73
AsuHPI GGTGA 3 cut(s) 205, 233, 521
BaeGI GKGCMC 1 cut(s) 76
BanII GRGCYC 1 cut(s) 76
BbvI GCAGC 1 cut(s) 391
BceAI ACGGC 1 cut(s) 417
BciT130I CCWGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 63
BfmI CTRYAG 1 cut(s) 273
BisI GCNGC 1 cut(s) 405
BlpI GCTNAGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 406
BmcAI AGTACT 1 cut(s) 518
Bme1390I CCNGG 1 cut(s) 210
BmgT120I GGNCC 2 cut(s) 72, 73
BmiI GGNNCC 2 cut(s) 73, 74
BmrFI CCNGG 1 cut(s) 210
BmsI GCATC 4 cut(s) 34, 113, 446, 503
BplI GAGNNNNNCTC 4 cut(s) 319, 351, 503, 535
BpmI CTGGAG 1 cut(s) 365
Bpu1102I GCTNAGC 1 cut(s) 48
BsaAI YACGTR 1 cut(s) 241
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 190
Bse1I ACTGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 210
BseGI GGATG 2 cut(s) 265, 494
BseLI CCNNNNNNNGG 2 cut(s) 189, 190
BseMII CTCAG 2 cut(s) 62, 99
BseNI ACTGG 1 cut(s) 279
BseRI GAGGAG 3 cut(s) 68, 327, 527
BseSI GKGCMC 1 cut(s) 76
BseXI GCAGC 1 cut(s) 391
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 390
BshFI GGCC 1 cut(s) 74
BslI CCNNNNNNNGG 2 cut(s) 189, 190
BsmAI GTCTC 1 cut(s) 63
BsmBI CGTCTC 1 cut(s) 63
BsnI GGCC 1 cut(s) 74
Bsp120I GGGCCC 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 2 cut(s) 139, 205
Bsp1720I GCTNAGC 1 cut(s) 48
BspACI CCGC 1 cut(s) 498
BspANI GGCC 1 cut(s) 74
BspCNI CTCAG 2 cut(s) 61, 100
BspLI GGNNCC 2 cut(s) 73, 74
BspPI GGATC 1 cut(s) 200
BspQI GCTCTTC 1 cut(s) 531
BsrI ACTGG 1 cut(s) 279
BssMI GATC 2 cut(s) 139, 205
Bst2UI CCWGG 1 cut(s) 210
Bst6I CTCTTC 3 cut(s) 40, 150, 531
BstBAI YACGTR 1 cut(s) 241
BstC8I GCNNGC 1 cut(s) 376
BstDEI CTNAG 3 cut(s) 48, 108, 170
BstF5I GGATG 2 cut(s) 265, 494
BstKTI GATC 2 cut(s) 142, 208
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 139, 205
BstNI CCWGG 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 208
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 76
BstV1I GCAGC 1 cut(s) 391
BsuRI GGCC 1 cut(s) 74
BtsCI GGATG 2 cut(s) 265, 494
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 72, 73
Csp6I GTAC 2 cut(s) 238, 517
CviAII CATG 3 cut(s) 62, 298, 543
CviJI RGCY 9 cut(s) 52, 74, 175, 182, 203, 278, 374, 404, 541
CviKI_1 RGCY 9 cut(s) 52, 74, 175, 182, 203, 278, 374, 404, 541
CviQI GTAC 2 cut(s) 238, 517
DdeI CTNAG 3 cut(s) 48, 108, 170
DpnI GATC 2 cut(s) 141, 207
DpnII GATC 2 cut(s) 139, 205
Eam1104I CTCTTC 3 cut(s) 40, 150, 531
EarI CTCTTC 3 cut(s) 40, 150, 531
Eco24I GRGCYC 1 cut(s) 76
EcoO109I RGGNCCY 2 cut(s) 72, 73
EcoRI GAATTC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 208
EcoT38I GRGCYC 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 3 cut(s) 65, 301, 546
FaiI YATR 7 cut(s) 63, 251, 299, 356, 445, 454, 544
FatI CATG 3 cut(s) 61, 297, 542
Fnu4HI GCNGC 1 cut(s) 405
FokI GGATG 2 cut(s) 252, 481
FriOI GRGCYC 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 405
GluI GCNGC 1 cut(s) 405
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 365
HaeIII GGCC 1 cut(s) 74
Hin1II CATG 3 cut(s) 65, 301, 546
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 3 cut(s) 83, 262, 412
HphI GGTGA 3 cut(s) 205, 233, 521
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 2 cut(s) 109, 417
Hpy188III TCNNGA 3 cut(s) 41, 137, 344
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 279
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 5 cut(s) 104, 297, 407, 437, 530
HpyF3I CTNAG 3 cut(s) 48, 108, 170
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 3 cut(s) 65, 301, 546
Kzo9I GATC 2 cut(s) 139, 205
LguI GCTCTTC 1 cut(s) 531
LmnI GCTCC 2 cut(s) 340, 491
LpnPI CCDG 6 cut(s) 195, 222, 292, 309, 329, 492
Lsp1109I GCAGC 1 cut(s) 391
LweI GCATC 4 cut(s) 34, 113, 446, 503
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 385
MalI GATC 2 cut(s) 141, 207
MboI GATC 2 cut(s) 139, 205
MboII GAAGA 6 cut(s) 27, 29, 164, 167, 173, 176
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 5 cut(s) 87, 145, 301, 418, 460
MlyI GAGTC 1 cut(s) 406
MmeI TCCRAC 1 cut(s) 489
MnlI CCTC 9 cut(s) 43, 86, 89, 151, 189, 321, 348, 389, 505
MslI CAYNNNNRTG 1 cut(s) 109
MspA1I CMGCKG 1 cut(s) 500
MspR9I CCNGG 1 cut(s) 210
MvaI CCWGG 1 cut(s) 210
NdeII GATC 2 cut(s) 139, 205
NlaIII CATG 3 cut(s) 65, 301, 546
NlaIV GGNNCC 2 cut(s) 73, 74
NmuCI GTSAC 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 531
PfeI GAWTC 2 cut(s) 83, 262
PkrI GCNGC 1 cut(s) 406
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
Ppu21I YACGTR 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 208
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 208
PspN4I GGNNCC 2 cut(s) 73, 74
PspOMI GGGCCC 1 cut(s) 72
PspPI GGNCC 2 cut(s) 72, 73
RsaI GTAC 2 cut(s) 239, 518
RsaNI GTAC 2 cut(s) 238, 517
RseI CAYNNNNRTG 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 531
SatI GCNGC 1 cut(s) 405
Sau3AI GATC 2 cut(s) 139, 205
Sau96I GGNCC 2 cut(s) 72, 73
ScaI AGTACT 1 cut(s) 518
SchI GAGTC 1 cut(s) 406
ScrFI CCNGG 1 cut(s) 210
SduI GDGCHC 1 cut(s) 76
SetI ASST 4 cut(s) 54, 205, 243, 332
SfaNI GCATC 4 cut(s) 34, 113, 446, 503
SfcI CTRYAG 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 109
Sse9I AATT 5 cut(s) 87, 145, 301, 418, 460
SsiI CCGC 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 208
TaiI ACGT 1 cut(s) 243
TaqI TCGA 3 cut(s) 81, 118, 521
TaqII GACCGA 1 cut(s) 300
TasI AATT 5 cut(s) 87, 145, 301, 418, 460
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 2 cut(s) 83, 262
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 1 cut(s) 404
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 3 cut(s) 30, 39, 314
TspRI CASTG 1 cut(s) 286
XapI RAATTY 2 cut(s) 418, 460
ZrmI AGTACT 1 cut(s) 518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.