MD03G1068500.v1.1
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010747
Physical Location & Seq
Reverse (-)
5566007 .. 5570327
4321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1068500.v1.1.491

Sequence Viewer

Length: 3315 bp
ATGCCGACGCCGGTGAGCGCAGCGAGGCAATGCTTGACGGACGAGGCGGCACGTGCTTTGGACGACGCCGTTGTTGTAGCGCGCAGGCGGAGCCACGCCCAGACGACGTCGTTACATGCCGTCTCGGCCTTGCTTTCCTTGCCGTCCTCGGCCCTCCGCGACGCCTGCGCCCGCGCCAGGAGCAGCGCGTACTCCCCACGCCTACAGTTCCGGGCTCTCGAGCTCTCCGTCGGAGTTTCCTTGGACAGGTTGCCGTCGTCCAAGGCACAGGACGAGCCTCCGGTCTCGAACTCGCTCATGGCGGCGATCAAGCGGTCTCAGGCGAACCAGAGGCGGCATCCGGCGAGCTTTCACTTGCACCAGATTCACAGCCAGCAGCAAGCGGCATCGCTTTTGAAGGTTGAGCTGAAACACTTTGTTTTATCTATTTTGGACGACCCGATTGTGAGTCGGGTCTTCGGAGAAGCCGGGTTTCGGAGCTGTGATATAAAGTTCGCGATTATTCACCCGCCGGTCACGCAATCCACCCGGTTTCGTCGGACCCGGTGCCCGCCCATTTTCCTGTGCAACTTAACGGACTCGGACCATGCCCGACCCGGATTCAGCTTCCCTTTTTCCGGGTTCGAAGACAGAGACGAGAACTCCAGAAGAATAGCCGACGTGTTGGTTAGAAAAAGCGGGAAAAATCCGTTGCTAATCGGCGTTTGCGCCGGCGACGCTCTGAAGAGCTTCACCGAGGCCGTGCAGAAGGGTAAAGCGGGGATTTTTCCAGCTGAAATCGATAACTTTAGCATGGTATCCATCGAGAAGGAGGTTTCTGAGTTCGTCGTCAATGGCGGAAGCGAGGAGGAGATGGGTTCCAAGTTTGAGGAGGTGGGTCGAATGGCTGCGCGTTGCTCTGGAGCTGGAAGTGGTGTTATTGTAAATATTGGGGATTTGAAGGGTTTAGTGGGTGAGGGTATGGTGGCGGAGGAAGCTCTGAGCTTTGTGGTGCTGCAGTTGAAGAGCTTGTTGGAGATTCATGGTGGGAAATTACGGTTGATTGGCGCGGCTGCGAGCCACGAAGTGTTTACAAAGCTTTCGCTTTGGTTTTCGACGATTGAGAAGGATTGGGATCTTCATCTTCTTCCCATTACTTCTTCTAAAGCTTCAATGGAAGGAGGTTACTCGAAATCCAGTTTGATGGGGTCCTTCGTTCCATTTGGCGGATTCTTTTCTGCACCATCCAACTTCAAAAATCCATTGAGCAGCACATATCGATCGTTTGGACGTTGTCATGGGTGCACAGAGAAGTATGAGCAAGAAATTGCTTCTGTTCTGAAAGTAGGATCAACTGTTTCGGTAACTGATCAGCGGTCAGATAGCTTGCCTTCTTGGTTACAAATGCACAAACTTGGCACTGGCAAGGGAGATGATCTAGCAAAGACCAAAGATGATAAAACAACAATGAATGTTACAGTATCAGCACTGCAGAAAAAATGGGATGATTTCTGCCGACAAAATCTTCATGCTCAACCATTCCCTAAAGTGGACATTTATCAAGCAGGGCGTCAAGTTGCATCTGCCGAGGGCTCCCTAGCTGTTTGGGATAGAAAAGAAAACAGTGGTGAAGACTCATCTCCAAATGAAAGTGGATGTGCTATTCAATTTCACTGCCAGCCAATGGTTATGCAAAAGAGTTATCTATCAAAACAGAACTTGCCTGTGCAAGTAGTTTCTGATGGTGAGAATACCAGTCTCCAGTCCGAGCTATTGGTAAAAGATTCGAAGGGTCAGCGAGTTGAACTGGGAAGTCCCTGCCTAACTACTTACCCCATCCACAATCTGCCCACCGACTACACATCATCTGCGTTGATCACTTCTGTGGCAACAGATTTGGGATTGGGAACATTATATGCAGCAACCAGCCAAGGGCCATTAAGTCCTCAATTACAAGATATTAAAGGGAGTTCCCACCACCTCTCTGGTTCCATTTCTGCTGAATTTGATGTATTGAGTGAAAATAGTTCACGCCAAATAGCTCAGTCCTCTTCCTGCTCTGCTTCTGATCTTGGAGGGCAGATTGATCCAAGCGATATCAAATCACTTACGAGAGTTCTCACAGAAAAGGTTGGCTGGCAAAATGAAGCCATTTGCAGTATCAGTCAAGCTGTGGCACGTTGCAAATCTGGTGGTGGAAGGAATCAGGGTTCAAAAGTTAGAGGAGATATATGGCTGACTTTGATTGGACCTGACAAAGTCGGAAAGAAGAAACTTGCTTTAGCACTTGCTGAGATACTTTTTGGCTCCAGAGAAAGCTTAATTTCTGTGGATCTGGATTCCCAAGACAGGGGTTACCAATCAAACTCAGTTTTTCAATCCGAATGCGCAGATGATTACAATTTGAAGTTTAGGGGTAAGACAGTTGTTGATTACGTTGCAGGGGAGTTAAGCAGGAGACCTCCTTCAGTGGTCTTCCTTGAAAATGTCGATAAGGCGGACTTTATTGCCCAGAGTAGCTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGACTCGCATGGGAGAGAAATCAGCATTAACGACATAATCTTCGTGACAACCTCCACTATAAAAAAGAGCAGCAAAAGCCACTCTGTAGAGATCGAACCCCATAAATTTTCTGAGGAGATCATACTTACCGCCGAGAAATGTCAAATGCAAATTCGGAATTTTGGAGATGCCAACCAAAGCAAGGGCATGAGTGTGAGGATTGCACCAAGAGATGGAACCTCGAACCCGTATTCTTCTGTAAATAAAAGAAAGCTGATTGACACCAATGCCTCCATAGAGCAATCCTCTGAACTACAGAAACGGTCCAACAAGCAATTAAGGAACTTTCTTGATTTGAATTTGCCAGTAGCGGAGACAGACGAGAACATAGATTCTGAAGATTGTGACGGTGACTCCATCTCCGAAAACTCGGAAGCTTGGTTGGAAGGTTTCCTCAATCAAGTGGACGGAAAAGTGGTTTTGAAGCCATTTGATTTCGATGCACTTGCTGAGAAAATAGTGAAGGAAATCAATCACGAATTTAAGAAAATATTCAGATATGAGGTTCAGCTGGAGATTGATTTCGGAGTCATGGTTCAGATGCTCGCAGCTGGCTGGTTATCGGACAAGAAGAAAGCCGTAGAGGAGTGGGTGGAACAAGTTCTCAGCCGAAGCTTTGTCGAAGCACGCCAGAAGTTCTGTCTCACTGCTCATCCAGTAATGAAACTGGCTGCTGCTGGAACTCTTTCCGTCGACGAGCAAGCTCCCGGCGTTTGCCTTCCTGCTAGAATTAGTCTGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1105

Amino Acids

120.54

Weight (kDa)

6.36

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NBD_SMAX1 PF23569 213 - 384 1.2e-13 SMAX1 nucleotide binding domain
AAA_2 PF07724 740 - 875 2.3e-13 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1002 - 1083 2.7e-28 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 2368
AccB1I GGYRCC 1 cut(s) 546
AccB7I CCANNNNNTGG 2 cut(s) 1663, 2744
AccI GTMKAC 1 cut(s) 3264
AccII CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
AclWI GGATC 4 cut(s) 1122, 1336, 2060, 2319
AcsI RAATTY 6 cut(s) 1982, 2636, 2682, 2689, 2869, 3050
AcuI CTGAAG 3 cut(s) 743, 2430, 2928
AcvI CACGTG 1 cut(s) 53
AcyI GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
AdeI CACNNNGTG 1 cut(s) 1066
AfaI GTAC 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 660
AjiI CACGTC 1 cut(s) 661
AjnI CCWGG 2 cut(s) 176, 2505
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AleI CACNNNNGTG 1 cut(s) 1862
AloI GAACNNNNNNTCC 4 cut(s) 2173, 2205, 3090, 3122
Alw21I GWGCWC 2 cut(s) 225, 1286
Alw26I GTCTC 8 cut(s) 127, 289, 321, 627, 1742, 2431, 2879, 3218
Alw44I GTGCAC 1 cut(s) 1282
AlwI GGATC 4 cut(s) 1122, 1336, 2060, 2319
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 5 cut(s) 126, 150, 738, 1913, 2508
ApaLI GTGCAC 1 cut(s) 1282
ApoI RAATTY 6 cut(s) 1982, 2636, 2682, 2689, 2869, 3050
ArsI GACNNNNNNTTYG 2 cut(s) 2555, 2587
Asp700I GAANNNNTTC 4 cut(s) 728, 3062, 3171, 3256
AspS9I GGNCC 7 cut(s) 151, 540, 583, 1188, 1913, 2228, 2835
AsuC2I CCSGG 7 cut(s) 212, 469, 529, 544, 597, 619, 3279
AsuHPI GGTGA 7 cut(s) 25, 497, 724, 965, 1619, 1736, 2933
AsuII TTCGAA 2 cut(s) 624, 1766
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 5 cut(s) 540, 583, 1188, 2228, 2835
BaeGI GKGCMC 2 cut(s) 551, 1286
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 3 cut(s) 217, 225, 1574
BbrPI CACGTG 1 cut(s) 53
BbsI GAAGAC 4 cut(s) 448, 633, 1617, 2446
Bbv12I GWGCWC 2 cut(s) 225, 1286
BccI CCATC 8 cut(s) 809, 847, 1177, 1231, 1715, 1823, 2738, 2936
BceAI ACGGC 6 cut(s) 53, 104, 127, 238, 725, 3134
BcgI CGANNNNNNTGC 2 cut(s) 2999, 3033
BciT130I CCWGG 2 cut(s) 178, 2507
BciVI GTATCC 1 cut(s) 808
BclI TGATCA 2 cut(s) 1348, 1854
BcnI CCSGG 7 cut(s) 212, 469, 529, 544, 597, 619, 3279
BcoDI GTCTC 8 cut(s) 127, 289, 321, 627, 1742, 2431, 2879, 3218
BfaI CTAG 3 cut(s) 1418, 1577, 3297
BfmI CTRYAG 5 cut(s) 203, 995, 1469, 2616, 2825
BfuI GTATCC 1 cut(s) 808
BglI GCCNNNNNGGC 1 cut(s) 125
Bme1390I CCNGG 9 cut(s) 178, 212, 469, 529, 544, 597, 619, 2507, 3279
Bme18I GGWCC 5 cut(s) 540, 583, 1188, 2228, 2835
BmeT110I CYCGRG 1 cut(s) 218
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 7 cut(s) 151, 540, 583, 1188, 1913, 2228, 2835
BmiI GGNNCC 9 cut(s) 92, 542, 548, 859, 1189, 1573, 1969, 2287, 2749
BmrFI CCNGG 9 cut(s) 178, 212, 469, 529, 544, 597, 619, 2507, 3279
BmrI ACTGGG 1 cut(s) 1796
BmsI GCATC 6 cut(s) 346, 395, 1568, 2689, 3001, 3102
BmuI ACTGGG 1 cut(s) 1796
BpiI GAAGAC 4 cut(s) 448, 633, 1617, 2446
BplI GAGNNNNNCTC 2 cut(s) 2801, 2833
BpmI CTGGAG 5 cut(s) 628, 921, 1724, 2272, 3104
Bpu14I TTCGAA 2 cut(s) 624, 1766
BpuMI CCSGG 7 cut(s) 212, 469, 529, 544, 597, 619, 3279
Bsa29I ATCGAT 2 cut(s) 780, 1258
BsaAI YACGTR 1 cut(s) 53
BsaBI GATNNNNATC 2 cut(s) 1113, 1119
BsaHI GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
BsaI GGTCTC 3 cut(s) 289, 321, 2431
BsaJI CCNNGG 7 cut(s) 147, 240, 261, 735, 1566, 1909, 2505
BsaWI WCCGGW 1 cut(s) 280
BsaXI ACNNNNNCTCC 8 cut(s) 626, 656, 2909, 2915, 2939, 2945, 3090, 3120
Bse118I RCCGGY 3 cut(s) 10, 511, 710
Bse1I ACTGG 9 cut(s) 1176, 1405, 1734, 1741, 1791, 2524, 2876, 3227, 3243
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 2 cut(s) 1113, 1119
BseBI CCWGG 2 cut(s) 178, 2507
BseCI ATCGAT 2 cut(s) 780, 1258
BseDI CCNNGG 7 cut(s) 147, 240, 261, 735, 1566, 1909, 2505
BseGI GGATG 6 cut(s) 337, 1223, 1489, 1640, 1815, 3223
BseJI GATNNNNATC 2 cut(s) 1113, 1119
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 9 cut(s) 332, 810, 971, 2036, 2262, 2361, 2634, 3012, 3190
BseNI ACTGG 9 cut(s) 1176, 1405, 1734, 1741, 1791, 2524, 2876, 3227, 3243
BsePI GCGCGC 1 cut(s) 80
BseRI GAGGAG 6 cut(s) 860, 863, 884, 2217, 2660, 3170
BseSI GKGCMC 2 cut(s) 551, 1286
BsgI GTGCAG 2 cut(s) 764, 1203
Bsh1236I CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
Bsh1285I CGRYCG 1 cut(s) 1262
BshFI GGCC 5 cut(s) 128, 152, 740, 1915, 2510
BshNI GGYRCC 1 cut(s) 546
BshVI ATCGAT 2 cut(s) 780, 1258
BsiEI CGRYCG 1 cut(s) 1262
BsiHKAI GWGCWC 2 cut(s) 225, 1286
BsiHKCI CYCGRG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 1779, 2488
BsmAI GTCTC 8 cut(s) 127, 289, 321, 627, 1742, 2431, 2879, 3218
BsmBI CGTCTC 2 cut(s) 127, 627
BsmFI GGGAC 2 cut(s) 1779, 2488
BsmI GAATGC 1 cut(s) 2369
BsnI GGCC 5 cut(s) 128, 152, 740, 1915, 2510
Bso31I GGTCTC 3 cut(s) 289, 321, 2431
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 2 cut(s) 624, 1766
Bsp1286I GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
Bsp68I TCGCGA 1 cut(s) 497
BspANI GGCC 5 cut(s) 128, 152, 740, 1915, 2510
BspCNI CTCAG 9 cut(s) 331, 811, 972, 2035, 2263, 2360, 2635, 3013, 3189
BspDI ATCGAT 2 cut(s) 780, 1258
BspFNI CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
BspLI GGNNCC 9 cut(s) 92, 542, 548, 859, 1189, 1573, 1969, 2287, 2749
BspMAI CTGCAG 2 cut(s) 999, 1473
BspPI GGATC 4 cut(s) 1122, 1336, 2060, 2319
BspQI GCTCTTC 2 cut(s) 719, 998
BspT104I TTCGAA 2 cut(s) 624, 1766
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 3 cut(s) 289, 321, 2431
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 3 cut(s) 10, 511, 710
BsrI ACTGG 9 cut(s) 1176, 1405, 1734, 1741, 1791, 2524, 2876, 3227, 3243
BssAI RCCGGY 3 cut(s) 10, 511, 710
BssECI CCNNGG 7 cut(s) 147, 240, 261, 735, 1566, 1909, 2505
BssHII GCGCGC 1 cut(s) 80
BssNI GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
BssT1I CCWWGG 3 cut(s) 240, 261, 1909
Bst2UI CCWGG 2 cut(s) 178, 2507
Bst4CI ACNGT 8 cut(s) 207, 1038, 1336, 1459, 1604, 2404, 2835, 2921
Bst6I CTCTTC 3 cut(s) 719, 998, 2035
BstACI GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
BstBAI YACGTR 1 cut(s) 53
BstBI TTCGAA 2 cut(s) 624, 1766
BstDEI CTNAG 9 cut(s) 318, 819, 980, 2022, 2271, 2347, 2643, 3021, 3176
BstEII GGTNACC 1 cut(s) 2333
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 6 cut(s) 337, 1223, 1489, 1640, 1815, 3223
BstFNI CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
BstMAI GTCTC 8 cut(s) 127, 289, 321, 627, 1742, 2431, 2879, 3218
BstMCI CGRYCG 1 cut(s) 1262
BstNI CCWGG 2 cut(s) 178, 2507
BstNSI RCATGY 1 cut(s) 119
BstPI GGTNACC 1 cut(s) 2333
BstSCI CCNGG 9 cut(s) 176, 210, 467, 527, 542, 595, 617, 2505, 3277
BstSFI CTRYAG 5 cut(s) 203, 995, 1469, 2616, 2825
BstSLI GKGCMC 2 cut(s) 551, 1286
BstUI CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
BstV2I GAAGAC 4 cut(s) 448, 633, 1617, 2446
BstX2I RGATCY 2 cut(s) 1114, 2311
BstXI CCANNNNNNTGG 2 cut(s) 1183, 1964
BstYI RGATCY 2 cut(s) 1114, 2311
Bsu15I ATCGAT 2 cut(s) 780, 1258
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 5 cut(s) 128, 152, 740, 1915, 2510
BsuTUI ATCGAT 2 cut(s) 780, 1258
BtgZI GCGATG 1 cut(s) 372
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 6 cut(s) 337, 1223, 1489, 1640, 1815, 3223
BtsI GCAGTG 3 cut(s) 1466, 1651, 3216
BtsIMutI CAGTG 6 cut(s) 1398, 1466, 1609, 1651, 2454, 3216
BtuMI TCGCGA 1 cut(s) 497
Cfr10I RCCGGY 3 cut(s) 10, 511, 710
Cfr13I GGNCC 7 cut(s) 151, 540, 583, 1188, 1913, 2228, 2835
ClaI ATCGAT 2 cut(s) 780, 1258
CseI GACGC 5 cut(s) 16, 74, 170, 725, 1538
Csp6I GTAC 1 cut(s) 190
CspCI CAANNNNNGTGG 2 cut(s) 2152, 2187
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 9 cut(s) 318, 819, 980, 2022, 2271, 2347, 2643, 3021, 3176
DraIII CACNNNGTG 1 cut(s) 1066
Eam1104I CTCTTC 3 cut(s) 719, 998, 2035
EarI CTCTTC 3 cut(s) 719, 998, 2035
EciI GGCGGA 5 cut(s) 103, 852, 983, 1221, 2492
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 240, 261, 1909
Eco24I GRGCYC 3 cut(s) 217, 225, 1574
Eco31I GGTCTC 3 cut(s) 289, 321, 2431
Eco32I GATATC 1 cut(s) 2077
Eco47I GGWCC 5 cut(s) 540, 583, 1188, 2228, 2835
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 3 cut(s) 743, 2430, 2928
Eco72I CACGTG 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 218
Eco91I GGTNACC 1 cut(s) 2333
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoO109I RGGNCCY 1 cut(s) 1188
EcoO65I GGTNACC 1 cut(s) 2333
EcoRII CCWGG 2 cut(s) 176, 2505
EcoRV GATATC 1 cut(s) 2077
EcoT14I CCWWGG 3 cut(s) 240, 261, 1909
EcoT38I GRGCYC 3 cut(s) 217, 225, 1574
ErhI CCWWGG 3 cut(s) 240, 261, 1909
Esp3I CGTCTC 2 cut(s) 127, 627
FalI AAGNNNNNCTT 2 cut(s) 2465, 2497
FaqI GGGAC 2 cut(s) 1779, 2488
FauI CCCGC 5 cut(s) 179, 516, 558, 671, 751
FbaI TGATCA 2 cut(s) 1348, 1854
FblI GTMKAC 1 cut(s) 3264
FokI GGATG 6 cut(s) 324, 1210, 1496, 1647, 1802, 3210
FriOI GRGCYC 3 cut(s) 217, 225, 1574
FspBI CTAG 3 cut(s) 1418, 1577, 3297
FspI TGCGCA 1 cut(s) 2368
GsuI CTGGAG 5 cut(s) 628, 921, 1724, 2272, 3104
HaeIII GGCC 5 cut(s) 128, 152, 740, 1915, 2510
HgaI GACGC 5 cut(s) 16, 74, 170, 725, 1538
Hin1I GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
HincII GTYRAC 1 cut(s) 3265
HindII GTYRAC 1 cut(s) 3265
HindIII AAGCTT 5 cut(s) 1076, 1146, 2296, 2946, 3184
HphI GGTGA 7 cut(s) 25, 497, 724, 965, 1619, 1736, 2933
Hpy166II GTNNAC 6 cut(s) 1071, 1284, 1531, 2009, 2977, 3265
Hpy8I GTNNAC 6 cut(s) 1071, 1284, 1531, 2009, 2977, 3265
HpyCH4III ACNGT 8 cut(s) 207, 1038, 1336, 1459, 1604, 2404, 2835, 2921
HpyCH4IV ACGT 6 cut(s) 52, 107, 660, 1270, 2158, 2415
HpyF3I CTNAG 9 cut(s) 318, 819, 980, 2022, 2271, 2347, 2643, 3021, 3176
HpySE526I ACGT 6 cut(s) 52, 107, 660, 1270, 2158, 2415
Hsp92I GRCGYC 5 cut(s) 8, 66, 107, 162, 1549
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
Ksp22I TGATCA 2 cut(s) 1348, 1854
LguI GCTCTTC 2 cut(s) 719, 998
LmnI GCTCC 7 cut(s) 90, 180, 477, 902, 1577, 2291, 3280
LweI GCATC 6 cut(s) 346, 395, 1568, 2689, 3001, 3102
MaeI CTAG 3 cut(s) 1418, 1577, 3297
MaeII ACGT 6 cut(s) 52, 107, 660, 1270, 2158, 2415
MflI RGATCY 2 cut(s) 1114, 2311
MhlI GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
MlyI GAGTC 6 cut(s) 457, 572, 1607, 2528, 2918, 3108
MmeI TCCRAC 7 cut(s) 211, 518, 993, 1251, 2221, 2862, 2934
MreI CGCCGGCG 1 cut(s) 710
MroNI GCCGGC 1 cut(s) 710
MroXI GAANNNNTTC 4 cut(s) 728, 3062, 3171, 3256
MseI TTAA 8 cut(s) 572, 1919, 1941, 2300, 2429, 2559, 2849, 3054
MslI CAYNNNNRTG 2 cut(s) 1862, 2723
MspA1I CMGCKG 4 cut(s) 773, 1354, 3082, 3122
MspR9I CCNGG 9 cut(s) 178, 212, 469, 529, 544, 597, 619, 2507, 3279
Mva1269I GAATGC 1 cut(s) 2369
MvaI CCWGG 2 cut(s) 178, 2507
MvnI CGCG 7 cut(s) 82, 159, 174, 188, 497, 892, 1049
NaeI GCCGGC 1 cut(s) 712
NciI CCSGG 7 cut(s) 212, 469, 529, 544, 597, 619, 3279
NgoMIV GCCGGC 1 cut(s) 710
NlaIV GGNNCC 9 cut(s) 92, 542, 548, 859, 1189, 1573, 1969, 2287, 2749
NmeAIII GCCGAG 4 cut(s) 104, 128, 1591, 2689
NmuCI GTSAC 4 cut(s) 514, 2575, 2915, 2921
NruI TCGCGA 1 cut(s) 497
NsbI TGCGCA 1 cut(s) 2368
NspI RCATGY 1 cut(s) 119
NspV TTCGAA 2 cut(s) 624, 1766
OliI CACNNNNGTG 1 cut(s) 1862
PaeR7I CTCGAG 1 cut(s) 218
PauI GCGCGC 1 cut(s) 80
PciSI GCTCTTC 2 cut(s) 719, 998
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 1 cut(s) 2369
PdiI GCCGGC 1 cut(s) 712
PdmI GAANNNNTTC 4 cut(s) 728, 3062, 3171, 3256
PfeI GAWTC 8 cut(s) 364, 600, 1018, 1209, 1763, 2182, 2318, 2903
PflFI GACNNNGTC 3 cut(s) 106, 1272, 2237
PflMI CCANNNNNTGG 2 cut(s) 1663, 2744
Ple19I CGATCG 1 cut(s) 1262
PleI GAGTC 6 cut(s) 456, 572, 1607, 2528, 2918, 3107
PmaCI CACGTG 1 cut(s) 53
PmlI CACGTG 1 cut(s) 53
PpsI GAGTC 6 cut(s) 456, 572, 1607, 2528, 2918, 3107
Ppu21I YACGTR 1 cut(s) 53
PpuMI RGGWCCY 1 cut(s) 1188
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 1188
Psp6I CCWGG 2 cut(s) 176, 2505
PspCI CACGTG 1 cut(s) 53
PspEI GGTNACC 1 cut(s) 2333
PspGI CCWGG 2 cut(s) 176, 2505
PspN4I GGNNCC 9 cut(s) 92, 542, 548, 859, 1189, 1573, 1969, 2287, 2749
PspPI GGNCC 7 cut(s) 151, 540, 583, 1188, 1913, 2228, 2835
PspPPI RGGWCCY 1 cut(s) 1188
PstI CTGCAG 2 cut(s) 999, 1473
PsuI RGATCY 2 cut(s) 1114, 2311
PsyI GACNNNGTC 3 cut(s) 106, 1272, 2237
PteI GCGCGC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 1262
PvuII CAGCTG 3 cut(s) 773, 3082, 3122
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 2 cut(s) 1862, 2723
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 3263
SapI GCTCTTC 2 cut(s) 719, 998
SaqAI TTAA 8 cut(s) 572, 1919, 1941, 2300, 2429, 2559, 2849, 3054
Sau96I GGNCC 7 cut(s) 151, 540, 583, 1188, 1913, 2228, 2835
SchI GAGTC 6 cut(s) 457, 572, 1607, 2528, 2918, 3108
ScrFI CCNGG 9 cut(s) 178, 212, 469, 529, 544, 597, 619, 2507, 3279
SduI GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
SfaNI GCATC 6 cut(s) 346, 395, 1568, 2689, 3001, 3102
SfcI CTRYAG 5 cut(s) 203, 995, 1469, 2616, 2825
Sfr274I CTCGAG 1 cut(s) 218
SfuI TTCGAA 2 cut(s) 624, 1766
SgrAI CRCCGGYG 2 cut(s) 10, 710
SgrDI CGTCGACG 1 cut(s) 3263
SinI GGWCC 5 cut(s) 540, 583, 1188, 2228, 2835
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 2 cut(s) 1862, 2723
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SspI AATATT 2 cut(s) 928, 3063
SspMI CTAG 3 cut(s) 1418, 1577, 3297
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 9 cut(s) 176, 210, 467, 527, 542, 595, 617, 2505, 3277
StyI CCWWGG 3 cut(s) 240, 261, 1909
TaaI ACNGT 8 cut(s) 207, 1038, 1336, 1459, 1604, 2404, 2835, 2921
TaiI ACGT 6 cut(s) 55, 110, 663, 1273, 2161, 2418
TauI GCSGC 5 cut(s) 50, 305, 337, 386, 1052
TfiI GAWTC 8 cut(s) 364, 600, 1018, 1209, 1763, 2182, 2318, 2903
Tru1I TTAA 8 cut(s) 572, 1919, 1941, 2300, 2429, 2559, 2849, 3054
Tru9I TTAA 8 cut(s) 572, 1919, 1941, 2300, 2429, 2559, 2849, 3054
TscAI CASTG 6 cut(s) 1405, 1473, 1609, 1658, 2454, 3223
TseFI GTSAC 4 cut(s) 514, 2575, 2915, 2921
Tsp45I GTSAC 4 cut(s) 514, 2575, 2915, 2921
TspDTI ATGAA 7 cut(s) 1010, 1109, 1463, 1496, 1641, 2139, 3248
TspGWI ACGGA 6 cut(s) 53, 217, 590, 678, 2994, 3250
TspRI CASTG 6 cut(s) 1405, 1473, 1609, 1658, 2454, 3223
Tth111I GACNNNGTC 3 cut(s) 106, 1272, 2237
Van91I CCANNNNNTGG 2 cut(s) 1663, 2744
VneI GTGCAC 1 cut(s) 1282
VpaK11BI GGWCC 5 cut(s) 540, 583, 1188, 2228, 2835
XagI CCTNNNNNAGG 1 cut(s) 244
XapI RAATTY 6 cut(s) 1982, 2636, 2682, 2689, 2869, 3050
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 1961
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 3264
XmnI GAANNNNTTC 4 cut(s) 728, 3062, 3171, 3256
XspI CTAG 3 cut(s) 1418, 1577, 3297
ZraI GACGTC 1 cut(s) 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.