MD11G1072900.v1.1
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010747
Physical Location & Seq
Reverse (-)
6190931 .. 6195294
4364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1072900.v1.1.491

Sequence Viewer

Length: 3324 bp
ATGCCGACGCCGGTGAGTGCAGCCAGGCAATGTTTGACGGACGAGGCGGCGCGTGCTTTAGACGACGCGGTTGCTGTAGCGCGTAGGCGGAGCCACGCCCAGACGACGTCGCTCCACGCCGTCTCGGCCTTGCTTTCCTTGCCGTCTTCGGCCCTCCGCGACGCATGCGCCCGCGCCAAGAGCAGCGCGTACTCTCCACGCCTTCAGTTCCGGGCTCTTGAGCTGTCTGTCGGAGTTTCCTTGGACAGGTTGCCGTCGTCGAAGGCGCAGGACGAGCCTCCCGTATCGAACTCGCTCATGGCGGCGATCAAGCGGTCCCAGGCGAACCAGAGGCGGCAACCGGAGAGCTTCCACTTGCACCATATTCACAGCCAGCAGCAAGCGGCCTCGCTTTTGAAGGTCGAGCTGAAACACTTCGTCATATCAATCTTGGACGATCCGATTGTCAGTCGGGTCTTCGGAGAAGCTGGGTTTCGAAGCTGTGACATAAAGGTCGCGATTATTCACCCGCCGGTGACTCAATCGGCTCGGTTTCCTCGGACCCGATGCCCGCCCATTTTCCTATGCAACTTAACGGACGCGGACCCCGCCCGACCCGGATTCAGCTTGCCGCTTTCCGGGTTCGAAGACGGAGACGAGAACTCGAGAAGAATCGCCGACTTGTTGGTTAAAAAGAGCGGGAAAAACCCGCTGCTACTCGGCGTCTGCGCCGCCGAGGCTCTGAAGAGCTTCACCGAGGCCGTGCAGAAGGGTAAAGCGGGGATTTTACCTGCTGAAGTGGCTAGCTTCAGCGTAGTTTCCATCGAGACGGAGATTTCTGAGTTCGTCCTCAATGGCGGAAGCAAGGAGGAGATGGGTTGCAAGTTTGAGGAGGTGGGTCGAATGGCGGAGCGCTGCTCTGGATCTGGAAGTGGTGTTATTGTAAATATTGGGGATTTGAAGGGTTTAGTGGGTGAGGGTGTGGTGGCGGAGGAAGCTCTGAGCTTTGTGGTGCTGCAGCTGAAGAGCTTGTTGGAGATCCATGGTGTAAAATTGCGGTTGATTGGCGGGGCGGCGAGCCATGAAGTGTTTAGGAAGCTTTTGCTTCGGTTTTCGACGATTGAGAAGGATTGGGATCTTCATCTTCTTCCCATTACTTCTTCTAAAGCTTCAATGGAAGGAGTTTACTCGAAATCCAGTTTGATGGGGTCCTTCGTTCCATTTGGCGGATTCTTTCCTGCACCGTCCGACTTCAAAAGTCCATTGAGCAGCGCATATCAATCCTTTAAACGTTGTCATCAGTGCACAGAGAAGTATGAGCAAGAAGGTGCTTCTGTTCTGAAAATAGGATCAACTAATTCAGCAGCTGATCAGCGGTCAGATAGTTTGCCTTCTTGGTTGCAAACGTGCGAACTTGACACTGGCAAGGGAGACGATCTAGCAAAGACCAAAGATGATAAAACAACAACGAATGTTACAGTATCTGCACTGCAGAAAAAATGGGATGATATCTGCCAACAAAATCATCACCCTCAACCATTCCCTAAAGTGGACGTTTATCAAGCAGGGCCTCAAGTTGCATCTGGTGAGGGCTCCCTAGCAGTCTGGAATAGAAAAGAAAACAGCGGTGAAGATTCATCTCTAAATGAAAGCGGACATGCTATTCATTATCGCTGCCAGCCGATGGACATGCAAACGAGTCTTCTATCAAAACAGAACTTGCCTATGCAAGTAGTTTCCGATGCTGCTGAGAATGCTAGCCTCCGGTCAGAGCTATTGATTAAAGATTCAAAGGGTCAGCAAGTTGAAATGAGAAGCTCCTGCCAGACTACTTACCCCATCCACAATATGAATCTTTCCACTGACCACACATCGTCTTCATCAGTTACTTCTGTAGCAACAGATTTGGGATTGGGAACATTGTATGGATCAACCAGTCAAGGGCCAAGAAGTCCTAAATTACAAGATATTAGAGAGAGTTCCAGACACCTCTCTGGTTCCATTTCTGCTGAATTTGATGCATTGAGTGAAAATAGTTCACGCCATATTGCCCGGTCCTCTTCCTGCTCTGCGTCTGATCTTGGAGGGCAGGTTGATCCAAGTGATATCAAATCACTTAGGAGAGTTCTCACAGAAAAGGTTGGCTGGCAAAATGAAGCCATTTGCAGTATTAGTCAAGCTGTGGCATGTTGCAAGTCTGGTAGTGGAAGGAATCAGTGTTCGAAACTCAGAGGAGATATATGGCTGACTTTGGTTGGACCTGACAAAGTCGGAAAGAAGAAAATTGCTTTAGCACTTGCTGAGATACTTTTTGGCTCCAGAGAAAGCTTAATTTCTGCTGATATGTGTTCCCAAGATAGGGGTTGCCAAACAAACTCTGTTTTTCAATCCGAAGGTGTAGATGATTACAATGTGAAGTTTAGGGGTAAGACAGTTGTTGATTATGTTGCAGGGGAGTTAAGCAGGAGACCTCATTCGGTGGTATTCCTTGAAAATGTCGATAAGGCGGACTTTCTTGCCCAGAGAAGCTTGTCCCAGGCCATTAGGACTGGAAAGTTTCCAGATTCGCATGGGAGAGAAATCAGCATTAACGACATAATCTTTGTGACTACCTCCACTATCAAAAGCAGCAGCAAAAGCCGCTCTGGAGAGAATGAACCCCATAAATTCTCCGAGGAGGTCATACTTGCCGCCAAGAAATGTCAAATGCAAATTCGGAATCTTGGGGATGCCAATCAAAGCAAGGGCATGAATGTGAGGATTGCACCAAGAGATGGAACCTCAAACCCGTCTTCTTCTACAAATAAAAGAAAGCTGATTGACACCAATGCCTCCTTAGAGCGATTCTCTGAACTACAGAAGCAGTCCAACAAGCAATTAAGGAACTTTCTCGATTTGAATCTGCCAGTAGCGGAGCCTGACAAGAACATAGATTCTGAAGATTGTGACAGCGACTCCATCTCCGAAAACTCTGAAGCTTGGCTGGAAGATTTCCTTGGTCGAGTGAACGAAAAAGTGGTTTTGAAGCCATTTGATTTTGAAGCACTCGCTGAGAAAATAGTGAAGGAAATCAACCGCGAATTGAAGAAAATCTTCGGATATGAGGTTCAGCTGGAGATTGATTTTGGAGTCATGGTGCAGATACTCGCAGCTGGCTGGTTATCGGACAAGAAGAAAGCCGTAGAGGAGTGGGTTGAACAAGTTCTCAGCAGAAGCTTTGTCGAAGCATGCCTGAAGTTCCGTCTCACTGCTCATTCTGTAATAAAATTGGCTGCCGCTGGAACCCTTTCCGTCGACGAACAAGCTCCCGGCGTTTGCCTTCCTGCTCGAATTGGTCTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1108

Amino Acids

120.51

Weight (kDa)

6.8

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 112 2.9e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 214 - 385 9.5e-15 SMAX1 nucleotide binding domain
AAA_2 PF07724 743 - 883 1.2e-16 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1005 - 1086 4.3e-28 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 491
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 778, 2059
AccB7I CCANNNNNTGG 2 cut(s) 1663, 2753
AccBSI CCGCTC 2 cut(s) 678, 2622
AccI GTMKAC 1 cut(s) 3273
AccII CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
AclI AACGTT 1 cut(s) 1270
AclWI GGATC 7 cut(s) 431, 910, 1012, 1122, 1336, 1915, 2069
AcsI RAATTY 3 cut(s) 1991, 2645, 2691
AcuI CTGAAG 8 cut(s) 188, 743, 772, 795, 1022, 2937, 2973, 3233
AcyI GRCGYC 3 cut(s) 8, 107, 702
AfaI GTAC 1 cut(s) 191
AfeI AGCGCT 1 cut(s) 893
AfiI CCNNNNNNNGG 9 cut(s) 246, 617, 1205, 1528, 1663, 1920, 2337, 2338, 2753
AjnI CCWGG 3 cut(s) 23, 318, 2514
AjuI GAANNNNNNNTTGG 4 cut(s) 995, 1027, 2958, 2990
AloI GAACNNNNNNTCC 4 cut(s) 2182, 2214, 3069, 3101
Alw21I GWGCWC 1 cut(s) 1286
Alw26I GTCTC 6 cut(s) 127, 627, 800, 1404, 2440, 3227
Alw44I GTGCAC 1 cut(s) 1282
AlwI GGATC 7 cut(s) 431, 910, 1012, 1122, 1336, 1915, 2069
AlwNI CAGNNNCTG 2 cut(s) 1346, 1463
Ama87I CYCGRG 1 cut(s) 643
Aor51HI AGCGCT 1 cut(s) 893
AoxI GGCC 7 cut(s) 126, 150, 384, 738, 1547, 1922, 2517
ApaLI GTGCAC 1 cut(s) 1282
ApoI RAATTY 3 cut(s) 1991, 2645, 2691
ArsI GACNNNNNNTTYG 2 cut(s) 2564, 2596
Asp700I GAANNNNTTC 5 cut(s) 413, 728, 3071, 3180, 3265
AspLEI GCGC 9 cut(s) 52, 82, 170, 176, 188, 268, 710, 894, 1253
AspS9I GGNCC 9 cut(s) 151, 315, 540, 583, 1188, 1547, 1922, 2034, 2237
AsuC2I CCSGG 5 cut(s) 212, 597, 619, 2032, 3288
AsuHPI GGTGA 8 cut(s) 25, 497, 526, 724, 965, 1499, 1577, 1619
AsuII TTCGAA 3 cut(s) 475, 624, 2201
AsuNHI GCTAGC 2 cut(s) 782, 1736
AvaI CYCGRG 1 cut(s) 643
AvaII GGWCC 6 cut(s) 315, 540, 583, 1188, 2034, 2237
BaeGI GKGCMC 1 cut(s) 1286
BanII GRGCYC 2 cut(s) 217, 1574
BbsI GAAGAC 6 cut(s) 138, 448, 633, 1673, 1848, 2763
Bbv12I GWGCWC 1 cut(s) 1286
BccI CCATC 7 cut(s) 809, 847, 1177, 1657, 1826, 2747, 2945
BceAI ACGGC 5 cut(s) 104, 127, 238, 725, 3143
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 1651, 1685
BciT130I CCWGG 3 cut(s) 25, 320, 2516
BclI TGATCA 1 cut(s) 1348
BcnI CCSGG 5 cut(s) 212, 597, 619, 2032, 3288
BcoDI GTCTC 6 cut(s) 127, 627, 800, 1404, 2440, 3227
BfaI CTAG 5 cut(s) 783, 1418, 1577, 1737, 3317
BfmI CTRYAG 5 cut(s) 75, 995, 1469, 1872, 2834
BfoI RGCGCY 1 cut(s) 895
BfuAI ACCTGC 2 cut(s) 778, 2059
BglI GCCNNNNNGGC 2 cut(s) 125, 716
Bme1390I CCNGG 8 cut(s) 25, 212, 320, 597, 619, 2032, 2516, 3288
Bme18I GGWCC 6 cut(s) 315, 540, 583, 1188, 2034, 2237
BmeT110I CYCGRG 1 cut(s) 643
BmgT120I GGNCC 9 cut(s) 151, 315, 540, 583, 1188, 1547, 1922, 2034, 2237
BmrFI CCNGG 8 cut(s) 25, 212, 320, 597, 619, 2032, 2516, 3288
BmsI GCATC 5 cut(s) 536, 1568, 1711, 1987, 2698
BmtI GCTAGC 2 cut(s) 786, 1740
BpiI GAAGAC 6 cut(s) 138, 448, 633, 1673, 1848, 2763
BplI GAGNNNNNCTC 4 cut(s) 881, 913, 2810, 2842
BpmI CTGGAG 3 cut(s) 2281, 2646, 3113
Bpu14I TTCGAA 3 cut(s) 475, 624, 2201
BpuEI CTTGAG 2 cut(s) 239, 1536
BpuMI CCSGG 5 cut(s) 212, 597, 619, 2032, 3288
BsaBI GATNNNNATC 4 cut(s) 1113, 1119, 2712, 2877
BsaHI GRCGYC 3 cut(s) 8, 107, 702
BsaI GGTCTC 1 cut(s) 2440
BsaJI CCNNGG 9 cut(s) 240, 318, 536, 714, 735, 1021, 2514, 2652, 2974
BsaWI WCCGGW 2 cut(s) 340, 1743
BsaXI ACNNNNNCTCC 8 cut(s) 2055, 2085, 2918, 2924, 2948, 2954, 3099, 3129
Bsc4I CCNNNNNNNGG 9 cut(s) 246, 617, 1205, 1528, 1663, 1920, 2337, 2338, 2753
Bse118I RCCGGY 2 cut(s) 10, 511
Bse1I ACTGG 5 cut(s) 1176, 1405, 1914, 2533, 2885
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 4 cut(s) 1113, 1119, 2712, 2877
BseBI CCWGG 3 cut(s) 25, 320, 2516
BseDI CCNNGG 9 cut(s) 240, 318, 536, 714, 735, 1021, 2514, 2652, 2974
BseGI GGATG 3 cut(s) 1489, 1818, 2713
BseJI GATNNNNATC 4 cut(s) 1113, 1119, 2712, 2877
BseLI CCNNNNNNNGG 9 cut(s) 246, 617, 1205, 1528, 1663, 1920, 2337, 2338, 2753
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 7 cut(s) 810, 971, 1719, 2221, 2271, 3021, 3199
BseNI ACTGG 5 cut(s) 1176, 1405, 1914, 2533, 2885
BseRI GAGGAG 5 cut(s) 863, 884, 2226, 2669, 3179
BseSI GKGCMC 1 cut(s) 1286
BseYI CCCAGC 1 cut(s) 467
BsgI GTGCAG 5 cut(s) 39, 764, 1203, 1449, 3137
Bsh1236I CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
BshFI GGCC 7 cut(s) 128, 152, 386, 740, 1549, 1924, 2519
BsiHKAI GWGCWC 1 cut(s) 1286
BsiHKCI CYCGRG 1 cut(s) 643
BsiSI CCGG 9 cut(s) 11, 211, 341, 512, 597, 618, 1744, 2032, 3288
BslFI GGGAC 2 cut(s) 301, 2497
BslI CCNNNNNNNGG 9 cut(s) 246, 617, 1205, 1528, 1663, 1920, 2337, 2338, 2753
BsmAI GTCTC 6 cut(s) 127, 627, 800, 1404, 2440, 3227
BsmBI CGTCTC 5 cut(s) 127, 627, 800, 1404, 3227
BsmFI GGGAC 2 cut(s) 301, 2497
BsmI GAATGC 1 cut(s) 1738
BsnI GGCC 7 cut(s) 128, 152, 386, 740, 1549, 1924, 2519
Bso31I GGTCTC 1 cut(s) 2440
BsoBI CYCGRG 1 cut(s) 643
Bsp119I TTCGAA 3 cut(s) 475, 624, 2201
Bsp1286I GDGCHC 3 cut(s) 217, 1286, 1574
Bsp19I CCATGG 1 cut(s) 1021
Bsp68I TCGCGA 1 cut(s) 497
BspANI GGCC 7 cut(s) 128, 152, 386, 740, 1549, 1924, 2519
BspCNI CTCAG 7 cut(s) 811, 972, 1720, 2220, 2272, 3022, 3198
BspFNI CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
BspMAI CTGCAG 2 cut(s) 999, 1473
BspMI ACCTGC 2 cut(s) 778, 2059
BspOI GCTAGC 2 cut(s) 786, 1740
BspPI GGATC 7 cut(s) 431, 910, 1012, 1122, 1336, 1915, 2069
BspQI GCTCTTC 2 cut(s) 719, 998
BspT104I TTCGAA 3 cut(s) 475, 624, 2201
BspTNI GGTCTC 1 cut(s) 2440
BsrBI CCGCTC 2 cut(s) 678, 2622
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 10, 511
BsrI ACTGG 5 cut(s) 1176, 1405, 1914, 2533, 2885
BssAI RCCGGY 2 cut(s) 10, 511
BssECI CCNNGG 9 cut(s) 240, 318, 536, 714, 735, 1021, 2514, 2652, 2974
BssNI GRCGYC 3 cut(s) 8, 107, 702
BssT1I CCWWGG 3 cut(s) 240, 1021, 2974
Bst2UI CCWGG 3 cut(s) 25, 320, 2516
Bst4CI ACNGT 3 cut(s) 1224, 1459, 2413
Bst6I CTCTTC 3 cut(s) 719, 998, 2044
BstACI GRCGYC 3 cut(s) 8, 107, 702
BstBI TTCGAA 3 cut(s) 475, 624, 2201
BstDEI CTNAG 9 cut(s) 819, 980, 1728, 2096, 2207, 2280, 2815, 3030, 3185
BstDSI CCRYGG 1 cut(s) 1021
BstENI CCTNNNNNAGG 1 cut(s) 244
BstF5I GGATG 3 cut(s) 1489, 1818, 2713
BstFNI CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
BstH2I RGCGCY 1 cut(s) 895
BstHHI GCGC 9 cut(s) 52, 82, 170, 176, 188, 268, 710, 894, 1253
BstMAI GTCTC 6 cut(s) 127, 627, 800, 1404, 2440, 3227
BstNI CCWGG 3 cut(s) 25, 320, 2516
BstNSI RCATGY 5 cut(s) 168, 1640, 1672, 2169, 3210
BstSCI CCNGG 8 cut(s) 23, 210, 318, 595, 617, 2030, 2514, 3286
BstSFI CTRYAG 5 cut(s) 75, 995, 1469, 1872, 2834
BstSLI GKGCMC 1 cut(s) 1286
BstUI CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
BstV2I GAAGAC 6 cut(s) 138, 448, 633, 1673, 1848, 2763
BstX2I RGATCY 3 cut(s) 902, 1017, 1114
BstXI CCANNNNNNTGG 1 cut(s) 1183
BstYI RGATCY 3 cut(s) 902, 1017, 1114
BsuRI GGCC 7 cut(s) 128, 152, 386, 740, 1549, 1924, 2519
BtgI CCRYGG 1 cut(s) 1021
BtsCI GGATG 3 cut(s) 1489, 1818, 2713
BtsI GCAGTG 2 cut(s) 1466, 3225
BtsIMutI CAGTG 6 cut(s) 1286, 1398, 1466, 1839, 2201, 3225
BtuMI TCGCGA 1 cut(s) 497
BveI ACCTGC 2 cut(s) 778, 2059
CaiI CAGNNNCTG 2 cut(s) 1346, 1463
CfoI GCGC 9 cut(s) 52, 82, 170, 176, 188, 268, 710, 894, 1253
Cfr10I RCCGGY 2 cut(s) 10, 511
Cfr13I GGNCC 9 cut(s) 151, 315, 540, 583, 1188, 1547, 1922, 2034, 2237
CseI GACGC 6 cut(s) 16, 74, 170, 587, 691, 2040
Csp6I GTAC 1 cut(s) 190
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 9 cut(s) 819, 980, 1728, 2096, 2207, 2280, 2815, 3030, 3185
DraI TTTAAA 1 cut(s) 1267
DrdI GACNNNNNNGTC 1 cut(s) 491
DseDI GACNNNNNNGTC 1 cut(s) 491
Eam1104I CTCTTC 3 cut(s) 719, 998, 2044
EarI CTCTTC 3 cut(s) 719, 998, 2044
EciI GGCGGA 6 cut(s) 103, 852, 902, 983, 1221, 2501
Eco130I CCWWGG 3 cut(s) 240, 1021, 2974
Eco24I GRGCYC 2 cut(s) 217, 1574
Eco31I GGTCTC 1 cut(s) 2440
Eco32I GATATC 2 cut(s) 1489, 2086
Eco47I GGWCC 6 cut(s) 315, 540, 583, 1188, 2034, 2237
Eco47III AGCGCT 1 cut(s) 893
Eco57I CTGAAG 8 cut(s) 188, 743, 772, 795, 1022, 2937, 2973, 3233
Eco88I CYCGRG 1 cut(s) 643
EcoNI CCTNNNNNAGG 1 cut(s) 244
EcoO109I RGGNCCY 2 cut(s) 1188, 1547
EcoRII CCWGG 3 cut(s) 23, 318, 2514
EcoRV GATATC 2 cut(s) 1489, 2086
EcoT14I CCWWGG 3 cut(s) 240, 1021, 2974
EcoT22I ATGCAT 1 cut(s) 2002
EcoT38I GRGCYC 2 cut(s) 217, 1574
ErhI CCWWGG 3 cut(s) 240, 1021, 2974
Esp3I CGTCTC 5 cut(s) 127, 627, 800, 1404, 3227
FalI AAGNNNNNCTT 6 cut(s) 2474, 2506, 2958, 2990, 3056, 3088
FaqI GGGAC 2 cut(s) 301, 2497
FauI CCCGC 8 cut(s) 179, 516, 558, 595, 671, 696, 751, 1040
FbaI TGATCA 1 cut(s) 1348
FblI GTMKAC 1 cut(s) 3273
FokI GGATG 3 cut(s) 1496, 1805, 2720
FriOI GRGCYC 2 cut(s) 217, 1574
FspBI CTAG 5 cut(s) 783, 1418, 1577, 1737, 3317
GlaI GCGC 9 cut(s) 51, 81, 169, 175, 187, 267, 709, 893, 1252
GsaI CCCAGC 1 cut(s) 471
GsuI CTGGAG 3 cut(s) 2281, 2646, 3113
HaeII RGCGCY 1 cut(s) 895
HaeIII GGCC 7 cut(s) 128, 152, 386, 740, 1549, 1924, 2519
HapII CCGG 9 cut(s) 11, 211, 341, 512, 597, 618, 1744, 2032, 3288
HgaI GACGC 6 cut(s) 16, 74, 170, 587, 691, 2040
HhaI GCGC 9 cut(s) 52, 82, 170, 176, 188, 268, 710, 894, 1253
Hin1I GRCGYC 3 cut(s) 8, 107, 702
Hin6I GCGC 9 cut(s) 50, 80, 168, 174, 186, 266, 708, 892, 1251
HinP1I GCGC 9 cut(s) 50, 80, 168, 174, 186, 266, 708, 892, 1251
HincII GTYRAC 1 cut(s) 3274
HindII GTYRAC 1 cut(s) 3274
HindIII AAGCTT 6 cut(s) 1076, 1146, 2305, 2506, 2955, 3193
HpaII CCGG 9 cut(s) 11, 211, 341, 512, 597, 618, 1744, 2032, 3288
HphI GGTGA 8 cut(s) 25, 497, 526, 724, 965, 1499, 1577, 1619
Hpy166II GTNNAC 6 cut(s) 1165, 1284, 1531, 2018, 2986, 3274
Hpy8I GTNNAC 6 cut(s) 1165, 1284, 1531, 2018, 2986, 3274
HpyCH4III ACNGT 3 cut(s) 1224, 1459, 2413
HpyCH4IV ACGT 4 cut(s) 107, 1270, 1385, 1533
HpyF3I CTNAG 9 cut(s) 819, 980, 1728, 2096, 2207, 2280, 2815, 3030, 3185
HpySE526I ACGT 4 cut(s) 107, 1270, 1385, 1533
Hsp92I GRCGYC 3 cut(s) 8, 107, 702
HspAI GCGC 9 cut(s) 50, 80, 168, 174, 186, 266, 708, 892, 1251
Ksp22I TGATCA 1 cut(s) 1348
LguI GCTCTTC 2 cut(s) 719, 998
LmnI GCTCC 8 cut(s) 90, 117, 889, 1577, 1802, 2300, 2893, 3289
LweI GCATC 5 cut(s) 536, 1568, 1711, 1987, 2698
MaeI CTAG 5 cut(s) 783, 1418, 1577, 1737, 3317
MaeII ACGT 4 cut(s) 107, 1270, 1385, 1533
MaeIII GTNAC 6 cut(s) 482, 514, 1453, 1864, 2584, 2924
MbiI CCGCTC 2 cut(s) 678, 2622
MflI RGATCY 3 cut(s) 902, 1017, 1114
MhlI GDGCHC 3 cut(s) 217, 1286, 1574
MlyI GAGTC 4 cut(s) 511, 1687, 2927, 3117
MmeI TCCRAC 6 cut(s) 211, 993, 1251, 2215, 2230, 2871
Mph1103I ATGCAT 1 cut(s) 2002
MroXI GAANNNNTTC 5 cut(s) 413, 728, 3071, 3180, 3265
MseI TTAA 9 cut(s) 572, 669, 1266, 1761, 2309, 2438, 2568, 2858, 3322
MslI CAYNNNNRTG 1 cut(s) 2732
MspA1I CMGCKG 8 cut(s) 691, 1000, 1346, 1354, 1605, 3091, 3131, 3257
MspI CCGG 9 cut(s) 11, 211, 341, 512, 597, 618, 1744, 2032, 3288
MspR9I CCNGG 8 cut(s) 25, 212, 320, 597, 619, 2032, 2516, 3288
Mva1269I GAATGC 1 cut(s) 1738
MvaI CCWGG 3 cut(s) 25, 320, 2516
MvnI CGCG 9 cut(s) 52, 68, 82, 159, 174, 188, 497, 581, 3057
NciI CCSGG 5 cut(s) 212, 597, 619, 2032, 3288
NcoI CCATGG 1 cut(s) 1021
NheI GCTAGC 2 cut(s) 782, 1736
NmeAIII GCCGAG 3 cut(s) 104, 678, 739
NmuCI GTSAC 4 cut(s) 482, 514, 2584, 2924
NruI TCGCGA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 2002
NspI RCATGY 5 cut(s) 168, 1640, 1672, 2169, 3210
NspV TTCGAA 3 cut(s) 475, 624, 2201
PaeI GCATGC 2 cut(s) 168, 3210
PaeR7I CTCGAG 1 cut(s) 643
PciSI GCTCTTC 2 cut(s) 719, 998
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PctI GAATGC 1 cut(s) 1738
PdmI GAANNNNTTC 5 cut(s) 413, 728, 3071, 3180, 3265
PflFI GACNNNGTC 2 cut(s) 106, 2246
PflMI CCANNNNNTGG 2 cut(s) 1663, 2753
PleI GAGTC 4 cut(s) 511, 1686, 2927, 3116
PpsI GAGTC 4 cut(s) 511, 1686, 2927, 3116
PpuMI RGGWCCY 1 cut(s) 1188
Psp1406I AACGTT 1 cut(s) 1270
Psp5II RGGWCCY 1 cut(s) 1188
Psp6I CCWGG 3 cut(s) 23, 318, 2514
PspFI CCCAGC 1 cut(s) 467
PspGI CCWGG 3 cut(s) 23, 318, 2514
PspPI GGNCC 9 cut(s) 151, 315, 540, 583, 1188, 1547, 1922, 2034, 2237
PspPPI RGGWCCY 1 cut(s) 1188
PstI CTGCAG 2 cut(s) 999, 1473
PstNI CAGNNNCTG 2 cut(s) 1346, 1463
PsuI RGATCY 3 cut(s) 902, 1017, 1114
PsyI GACNNNGTC 2 cut(s) 106, 2246
PvuII CAGCTG 4 cut(s) 1000, 1346, 3091, 3131
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 1 cut(s) 2732
SalI GTCGAC 1 cut(s) 3272
SapI GCTCTTC 2 cut(s) 719, 998
SaqAI TTAA 9 cut(s) 572, 669, 1266, 1761, 2309, 2438, 2568, 2858, 3322
Sau96I GGNCC 9 cut(s) 151, 315, 540, 583, 1188, 1547, 1922, 2034, 2237
SchI GAGTC 4 cut(s) 511, 1687, 2927, 3117
ScrFI CCNGG 8 cut(s) 25, 212, 320, 597, 619, 2032, 2516, 3288
SduI GDGCHC 3 cut(s) 217, 1286, 1574
SfaNI GCATC 5 cut(s) 536, 1568, 1711, 1987, 2698
SfcI CTRYAG 5 cut(s) 75, 995, 1469, 1872, 2834
Sfr274I CTCGAG 1 cut(s) 643
SfuI TTCGAA 3 cut(s) 475, 624, 2201
SgrAI CRCCGGYG 2 cut(s) 10, 511
SgrDI CGTCGACG 1 cut(s) 3272
SinI GGWCC 6 cut(s) 315, 540, 583, 1188, 2034, 2237
SlaI CTCGAG 1 cut(s) 643
SmiMI CAYNNNNRTG 1 cut(s) 2732
SmlI CTYRAG 3 cut(s) 218, 643, 1551
SmoI CTYRAG 3 cut(s) 218, 643, 1551
SphI GCATGC 2 cut(s) 168, 3210
SspI AATATT 1 cut(s) 928
SspMI CTAG 5 cut(s) 783, 1418, 1577, 1737, 3317
StyD4I CCNGG 8 cut(s) 23, 210, 318, 595, 617, 2030, 2514, 3286
StyI CCWWGG 3 cut(s) 240, 1021, 2974
TaaI ACNGT 3 cut(s) 1224, 1459, 2413
TaiI ACGT 4 cut(s) 110, 1273, 1388, 1536
Tru1I TTAA 9 cut(s) 572, 669, 1266, 1761, 2309, 2438, 2568, 2858, 3322
Tru9I TTAA 9 cut(s) 572, 669, 1266, 1761, 2309, 2438, 2568, 2858, 3322
TscAI CASTG 6 cut(s) 1286, 1405, 1473, 1846, 2201, 3232
TseFI GTSAC 4 cut(s) 482, 514, 2584, 2924
Tsp45I GTSAC 4 cut(s) 482, 514, 2584, 2924
TspGWI ACGGA 6 cut(s) 53, 590, 645, 824, 3209, 3259
TspRI CASTG 6 cut(s) 1286, 1405, 1473, 1846, 2201, 3232
Tth111I GACNNNGTC 2 cut(s) 106, 2246
Van91I CCANNNNNTGG 2 cut(s) 1663, 2753
VneI GTGCAC 1 cut(s) 1282
VpaK11BI GGWCC 6 cut(s) 315, 540, 583, 1188, 2034, 2237
XagI CCTNNNNNAGG 1 cut(s) 244
XapI RAATTY 3 cut(s) 1991, 2645, 2691
XceI RCATGY 5 cut(s) 168, 1640, 1672, 2169, 3210
XhoI CTCGAG 1 cut(s) 643
XmiI GTMKAC 1 cut(s) 3273
XmnI GAANNNNTTC 5 cut(s) 413, 728, 3071, 3180, 3265
XspI CTAG 5 cut(s) 783, 1418, 1577, 1737, 3317
ZraI GACGTC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 2002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.