RLG00000036105
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010747
Physical Location & Seq
Forward (+)
75783012 .. 75788115
5104 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036105

Sequence Viewer

Length: 3324 bp
ATGCCGACGCCGGTGAGCGCAGCCAGGCAATGTTTGACGGAGGAGGCGGCGCGTGCGCTGGACGACGCAGTGGCCGTGGCGCGTAGGAGGAGCCACGCGCAGACGACGTCGCTTCATGCGGTCTCGGCTCTGCTCTCCCTGCCGTCGTCTGCTCTCCGAGACGCCTGCGCACGCGCCAGGAGCGGCGCGTACCCGTACACGCCGCGGCTCCAGTTCCGGGCGCTGGAGCTGTCCGTCGGGGTGTCGCTGGACCGGCTGCCGTCGTCGTCGAAGGCGCAGGAGGAGCCTCCGGTGTCGAACTCGCTGATGGCGGCGATCAAGCGGTCGCAGGCGAACCAGAGGCGGCACCCGGAGAGCTTCCACCTGCACCAGATCCACAGCCAGCAGCAGACGGCGTCGCTTCTCAAGGTCGAGCTCAAGCACTTCATGCTGTCCATTCTCGACGACCCGATTGTGAGTCGGGTCTTCGGGGAGGCCGGGTTCCGGAGCTGTGACATAAAGCTCGCGATTGTTCACCCGCCGGTGACGCCGCAATCCAATCGGCTCCCTCGGACTCTGTGCCCGCCCATTTTCCTATGCAACCTAACGGACTCGGACCCCGCTGCCCGTCGGAGATTCCCTTTCCCGTTCTCGGGATTCGACGAAAAGGACGAGAACTGCAAGAGAATCGGGGACGTGTTAGTTAGAAAGAGCGGAAAAAATCCGTTACTGATCGGCGTTTCCGCCGTTGAAGCTCTCAAAAGCTTCATTGAAGCTGTACAGAAAGGCAGAGCCTCCCTTCTGCCTCCTGAGGTAAGTAGGTTTAGTGTAGTTAGTATGGAAGAGGAGATTTCGGAGTTTGTGGTTGAGGGTGGGAGTGAAGAGAAGATGGGGTTGAAGTTGAAGGAGGTGGGTCATTTGGCTCAGCAATGCTCTGGAGATGGAGGAGCTGGTGTGATTGTCAACTTTGGGGAAATGAAGGCTTTGGTTGATGAGGGGGTGTTGAGTGATGCTTTGAGCTTTGTGGTGGTTCAGTTGAAGGGGTTGCTGGAGATTCATAGTGGGAAGTTGTGGTTGATCGGCGCGGCGGGGAGTTACGACATGTATATGAAACTTTTGGCTCGGTTTCCGGCTATTGAGAAGGATTGGGACCTTCATCTCCTTCCCATTAGCTCTTCTAAAGCTTCAGTCGATGGAGTCTACTCTAAATCCAGCTTGATGGGGTCCTTTGTTCCATTTGGGGGATTCTTCTCTGGACCATCTGACTTTACAAATCCACTGAGCAGCACGAATCAATCTTTTACACGATGCCATCAATGCACAGAGAAGTATGAACAAGAAGTTGCTTCTGTTCGGAAAGATGGATCAGCCATTACAGTTGGTGATCAGTGCTCAACTAGCTCACCTTCTTGGTTGCAAACGACTGAACTTGACCCTGGCAAGGGAGTGGATCTTGCAAAGACCAAAGCAGATAATACGACATTGAGTGAAACAGTATTGGGACTGCAAAAGAAATGGAATGATATCTGCCGAAAAATTCATCATGGTCAACCATTCCCTAAAATGGACAATTGTCAAGCTGGGTCTCATGTTGCATCTCCTGAGGGCTCTCATACTGCTGCAGATAGAAGAGAAAGCAGCGGTGAAGATTCATCTATACAGGAAAGCCGAAGTGCAAAACATCACTGCCTTCCAATGGACATGCAAAAGAGTTTTCTGTCAAAACAGAACTTAGTAATGCGAGTAGCTTCTGATGCTGAGTATGCTGGTATGCAATCCAAGCAATTGATCAGAGATTCAAAGGGTCAGCAACTTGAACTGGGAAGCCCCTGCCGTTCACCTTTCCCTATCCCCACTATGAATCTTCCCACTGATCGCACATCATCCTCATCTATCACTTCGGTGACCACAGATTTAGGATTGGGAACCTTATATGCATCAACTAGTCAGGGACCAAGAAGTCCCAAGTTGCAAGATCATAGAGAGTGTCTCCGACACCTTTCTGGTTCCATTTCTGCTGAATTTGATGCATTAAGTGAGAATAGTCCGCACCAAATAGCCCAGTCCTCTTCCTGCTCTGGATCTAATTTTGGTGGGCAGTTTGATCCAAGAGATTTCAAGTCACTTAGGAGAGTTCTCACAGAAAAGGTTTGCTGGCAAGATGAAGCAATATGCACCATTAGTGAAGCTATATCCCGGTGCAGGTCTGGTGGTGGAAGGCATCGTCGTTCAAAAGGCAGAGGAGATATATGGTTGACTTTGATTGGACCTGACAGGGTTGGAAAGAAGAAAATTGCTATAGCACTTGCAGAGTTAATGTTTGGCACTAGGGAAAGCCTAATCTCTGTTGATATGGGGGAGAGGGGATGCCAATCTGACTCGATTTTTCAATGGGAATCCCAAGATGATTATGATGTGAAGTTTAGGGGTAAGACGGCTGTAGATTATGTTGCTGGGGAATTAAGCAGGAGACCCCATTCAGTTGTCTTCCTTGAAAATGTAGATAAGGCTGATTTTCTGGCCCAGAGTAACTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGACTCTCACGGCAGAGAAATCAGCATTAACAACATGATCTTTGTAACGACTTCAGCTACCAAAAAGGGCAGCAAAAATCACTGCTTGGAGAATGAACCCCTGAAATTCTCAGAGGAGATAGTACTTGGTGCCAAGAGGTGTCAAATGCAAATACTGAATATTGGGGATGCCAGCCAAACGAAGGGCGTGAATGTGAGGATTGCACCAAGAGAAGGAACCTTAAACTCATCTTCTGTAAATAAGAGAAAGCTGATTGACAGCAGTGCCTCCATAGAGGAAGCCTCAGAGTTACAGAAACGGGGCAACAAGGTATCAAGGTCCTTTCTGGATTTGAATCTGCCTTTTGAAGAGATAGACGAGGGAATGAACTGTGGAGATTATGACAGTGACTCCATCTCCGAAAACTCAGAAGCCTGGATGGAAGACTTCCTTGATCAAGTTGATGAAGCAGTGGTTTTGAAGCCATTTAAGTTCGACGCTCTTGCTGAGAAATTAGTGAAGGAAATCAACCAAGAATTTAAGAAAGTCTTTGGATCTGAGTATCAGCTGGAGATTGACTTTGGAGTCATGGTTCAATTACTTGCAGCTTGTTGGTTATCAGACAAGAAGAAAGCAGTGGCGGAGTGGATTGAACAGGTTCTCTCCAGAAGTTTTGCTGAAGCACGTCAGAGTTATCGTCTCACTGCTCATTCTGTAATAAAATTGGTTGCTGGTGGAGCACTTTCGTTGCAAGAGCAGACTCCCGGTGTTTGCCTTCCTGCTAGAATTAGTCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1108

Amino Acids

121.4

Weight (kDa)

6.72

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 116 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 216 - 388 5e-16 SMAX1 nucleotide binding domain
AAA_2 PF07724 746 - 874 1e-13 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1006 - 1085 5.4e-30 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 372
AasI GACNNNNNNGTC 2 cut(s) 1938, 3107
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 2 cut(s) 372, 2172
AccB1I GGYRCC 2 cut(s) 345, 2672
AccBSI CCGCTC 2 cut(s) 183, 693
AccI GTMKAC 1 cut(s) 1179
AccII CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
AccIII TCCGGA 1 cut(s) 483
AclWI GGATC 6 cut(s) 367, 1351, 1437, 2068, 2078, 3085
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 4 cut(s) 1515, 2000, 2648, 3059
AcuI CTGAAG 3 cut(s) 1149, 2580, 3222
AcyI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
AfaI GTAC 4 cut(s) 191, 197, 759, 2667
AflIII ACRYGT 2 cut(s) 675, 1080
AhlI ACTAGT 1 cut(s) 1922
AjiI CACGTC 2 cut(s) 676, 3209
AjnI CCWGG 5 cut(s) 23, 176, 1414, 2517, 2957
AleI CACNNNNGTG 1 cut(s) 1880
AloI GAACNNNNNNTCC 4 cut(s) 464, 496, 3099, 3131
Alw21I GWGCWC 3 cut(s) 417, 1373, 3265
Alw26I GTCTC 6 cut(s) 127, 153, 1569, 1973, 2443, 3227
AlwI GGATC 6 cut(s) 367, 1351, 1437, 2068, 2078, 3085
Ama87I CYCGRG 1 cut(s) 631
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 4 cut(s) 72, 474, 2499, 2520
ApeKI GCWGC 9 cut(s) 20, 256, 385, 602, 1263, 1598, 1617, 2613, 3128
ApoI RAATTY 4 cut(s) 1515, 2000, 2648, 3059
Asp700I GAANNNNTTC 1 cut(s) 3180
AspS9I GGNCC 9 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2500, 2862
AsuC2I CCSGG 5 cut(s) 218, 350, 478, 2176, 3288
AsuHPI GGTGA 8 cut(s) 25, 506, 535, 1373, 1374, 1634, 1809, 1894
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 8 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2862
AxyI CCTNAGG 2 cut(s) 789, 1581
BaeGI GKGCMC 1 cut(s) 563
BanI GGYRCC 2 cut(s) 345, 2672
BanII GRGCYC 2 cut(s) 417, 1589
BbsI GAAGAC 3 cut(s) 457, 2458, 2973
Bbv12I GWGCWC 3 cut(s) 417, 1373, 3265
BbvI GCAGC 9 cut(s) 32, 243, 397, 589, 1275, 1585, 1629, 2625, 3140
BceAI ACGGC 8 cut(s) 59, 127, 244, 408, 710, 1797, 2430, 2569
BcgI CGANNNNNNTGC 8 cut(s) 147, 181, 521, 555, 649, 683, 3008, 3042
BciT130I CCWGG 5 cut(s) 25, 178, 1416, 2519, 2959
BclI TGATCA 3 cut(s) 1363, 1767, 2977
BcnI CCSGG 5 cut(s) 218, 350, 478, 2176, 3288
BcoDI GTCTC 6 cut(s) 127, 153, 1569, 1973, 2443, 3227
BcuI ACTAGT 1 cut(s) 1922
BfaI CTAG 4 cut(s) 1377, 1923, 2307, 3306
BfmI CTRYAG 3 cut(s) 1599, 2277, 2418
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 2 cut(s) 372, 2172
BlpI GCTNAGC 1 cut(s) 903
BmcAI AGTACT 1 cut(s) 2667
Bme18I GGWCC 8 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2862
BmeT110I CYCGRG 1 cut(s) 631
BmgBI CACGTC 2 cut(s) 676, 3209
BmgT120I GGNCC 9 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2500, 2862
BmrI ACTGGG 2 cut(s) 1808, 2035
BmsI GCATC 9 cut(s) 979, 1277, 1583, 1723, 1925, 1996, 2209, 2336, 2701
BmuI ACTGGG 2 cut(s) 1808, 2035
BoxI GACNNNNGTC 1 cut(s) 1551
BpiI GAAGAC 3 cut(s) 457, 2458, 2973
BplI GAGNNNNNCTC 4 cut(s) 1953, 1985, 2810, 2842
BpmI CTGGAG 6 cut(s) 194, 245, 936, 1049, 3113, 3172
Bpu1102I GCTNAGC 1 cut(s) 903
BpuEI CTTGAG 2 cut(s) 389, 401
BpuMI CCSGG 5 cut(s) 218, 350, 478, 2176, 3288
BsaBI GATNNNNATC 2 cut(s) 2350, 2877
BsaHI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BsaI GGTCTC 3 cut(s) 127, 1569, 2443
BsaJI CCNNGG 5 cut(s) 75, 203, 548, 1414, 2517
BsaWI WCCGGW 2 cut(s) 289, 483
Bse118I RCCGGY 3 cut(s) 10, 252, 520
Bse1I ACTGG 4 cut(s) 211, 1803, 2041, 2536
Bse21I CCTNAGG 2 cut(s) 789, 1581
Bse3DI GCAATG 2 cut(s) 35, 914
Bse8I GATNNNNATC 2 cut(s) 2350, 2877
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 5 cut(s) 25, 178, 1416, 2519, 2959
BseDI CCNNGG 5 cut(s) 75, 203, 548, 1414, 2517
BseGI GGATG 4 cut(s) 1862, 2351, 2716, 2967
BseJI GATNNNNATC 2 cut(s) 2350, 2877
BseMI GCAATG 2 cut(s) 35, 914
BseNI ACTGG 4 cut(s) 211, 1803, 2041, 2536
BseRI GAGGAG 7 cut(s) 56, 103, 296, 839, 939, 2235, 2672
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 9 cut(s) 32, 243, 397, 589, 1275, 1585, 1629, 2625, 3140
BseYI CCCAGC 2 cut(s) 1559, 2432
BsgI GTGCAG 2 cut(s) 350, 2200
Bsh1236I CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
Bsh1285I CGRYCG 1 cut(s) 326
BshFI GGCC 4 cut(s) 74, 476, 2501, 2522
BshNI GGYRCC 2 cut(s) 345, 2672
BsiEI CGRYCG 1 cut(s) 326
BsiHKAI GWGCWC 3 cut(s) 417, 1373, 3265
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 6 cut(s) 686, 1142, 1494, 1926, 1944, 2500
BsmAI GTCTC 6 cut(s) 127, 153, 1569, 1973, 2443, 3227
BsmBI CGTCTC 2 cut(s) 153, 3227
BsmFI GGGAC 6 cut(s) 686, 1142, 1494, 1926, 1944, 2500
BsnI GGCC 4 cut(s) 74, 476, 2501, 2522
Bso31I GGTCTC 3 cut(s) 127, 1569, 2443
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 5 cut(s) 417, 563, 1373, 1589, 3265
Bsp13I TCCGGA 1 cut(s) 483
Bsp1407I TGTACA 1 cut(s) 757
Bsp1720I GCTNAGC 1 cut(s) 903
Bsp68I TCGCGA 1 cut(s) 506
BspANI GGCC 4 cut(s) 74, 476, 2501, 2522
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
BspMAI CTGCAG 1 cut(s) 1603
BspMI ACCTGC 2 cut(s) 372, 2172
BspPI GGATC 6 cut(s) 367, 1351, 1437, 2068, 2078, 3085
BspQI GCTCTTC 1 cut(s) 1159
BspT107I GGYRCC 2 cut(s) 345, 2672
BspTNI GGTCTC 3 cut(s) 127, 1569, 2443
BsrBI CCGCTC 2 cut(s) 183, 693
BsrDI GCAATG 2 cut(s) 35, 914
BsrFI RCCGGY 3 cut(s) 10, 252, 520
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 4 cut(s) 211, 1803, 2041, 2536
BssAI RCCGGY 3 cut(s) 10, 252, 520
BssECI CCNNGG 5 cut(s) 75, 203, 548, 1414, 2517
BssNI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Bst2UI CCWGG 5 cut(s) 25, 178, 1416, 2519, 2959
Bst4CI ACNGT 4 cut(s) 1357, 1474, 2915, 2930
Bst6I CTCTTC 6 cut(s) 816, 855, 1159, 1603, 2053, 2886
BstACI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BstAPI GCANNNNNTGC 1 cut(s) 427
BstAUI TGTACA 1 cut(s) 757
BstC8I GCNNGC 9 cut(s) 54, 166, 172, 330, 383, 504, 563, 2135, 2716
BstDSI CCRYGG 2 cut(s) 75, 203
BstEII GGTNACC 1 cut(s) 1882
BstF5I GGATG 4 cut(s) 1862, 2351, 2716, 2967
BstFNI CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
BstH2I RGCGCY 1 cut(s) 224
BstMAI GTCTC 6 cut(s) 127, 153, 1569, 1973, 2443, 3227
BstMCI CGRYCG 1 cut(s) 326
BstNI CCWGG 5 cut(s) 25, 178, 1416, 2519, 2959
BstNSI RCATGY 2 cut(s) 1084, 1684
BstPAI GACNNNNGTC 1 cut(s) 1551
BstPI GGTNACC 1 cut(s) 1882
BstSFI CTRYAG 3 cut(s) 1599, 2277, 2418
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
BstV1I GCAGC 9 cut(s) 32, 243, 397, 589, 1275, 1585, 1629, 2625, 3140
BstV2I GAAGAC 3 cut(s) 457, 2458, 2973
BstX2I RGATCY 4 cut(s) 372, 1429, 2060, 3077
BstXI CCANNNNNNTGG 1 cut(s) 1198
BstYI RGATCY 4 cut(s) 372, 1429, 2060, 3077
Bsu36I CCTNAGG 2 cut(s) 789, 1581
BsuRI GGCC 4 cut(s) 74, 476, 2501, 2522
BtgI CCRYGG 2 cut(s) 75, 203
BtrI CACGTC 2 cut(s) 676, 3209
BtsCI GGATG 4 cut(s) 1862, 2351, 2716, 2967
BtsI GCAGTG 7 cut(s) 75, 1663, 2623, 2812, 3000, 3165, 3225
BtuMI TCGCGA 1 cut(s) 506
BveI ACCTGC 2 cut(s) 372, 2172
Cac8I GCNNGC 9 cut(s) 54, 166, 172, 330, 383, 504, 563, 2135, 2716
Cfr10I RCCGGY 3 cut(s) 10, 252, 520
Cfr13I GGNCC 9 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2500, 2862
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 6 cut(s) 16, 74, 170, 384, 535, 3029
Csp6I GTAC 4 cut(s) 190, 196, 758, 2666
CviAII CATG 8 cut(s) 116, 427, 1081, 1523, 1568, 1681, 2578, 3112
CviQI GTAC 4 cut(s) 190, 196, 758, 2666
DrdI GACNNNNNNGTC 2 cut(s) 1938, 3107
DseDI GACNNNNNNGTC 2 cut(s) 1938, 3107
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 6 cut(s) 816, 855, 1159, 1603, 2053, 2886
EarI CTCTTC 6 cut(s) 816, 855, 1159, 1603, 2053, 2886
EciI GGCGGA 2 cut(s) 712, 3179
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 417, 1589
Eco31I GGTCTC 3 cut(s) 127, 1569, 2443
Eco32I GATATC 1 cut(s) 1504
Eco47I GGWCC 8 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2862
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 3 cut(s) 1149, 2580, 3222
Eco81I CCTNAGG 2 cut(s) 789, 1581
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 1882
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 3 cut(s) 1129, 1203, 2862
EcoO65I GGTNACC 1 cut(s) 1882
EcoRII CCWGG 5 cut(s) 23, 176, 1414, 2517, 2957
EcoRV GATATC 1 cut(s) 1504
EcoT22I ATGCAT 2 cut(s) 1918, 2011
EcoT38I GRGCYC 2 cut(s) 417, 1589
Esp3I CGTCTC 2 cut(s) 153, 3227
FaeI CATG 8 cut(s) 119, 430, 1084, 1526, 1571, 1684, 2581, 3115
FalI AAGNNNNNCTT 4 cut(s) 2958, 2990, 3056, 3088
FaqI GGGAC 6 cut(s) 686, 1142, 1494, 1926, 1944, 2500
FatI CATG 8 cut(s) 115, 426, 1080, 1522, 1567, 1680, 2577, 3111
FauI CCCGC 4 cut(s) 525, 570, 607, 1060
FbaI TGATCA 3 cut(s) 1363, 1767, 2977
FblI GTMKAC 1 cut(s) 1179
FokI GGATG 4 cut(s) 1849, 2358, 2723, 2974
FriOI GRGCYC 2 cut(s) 417, 1589
FspBI CTAG 4 cut(s) 1377, 1923, 2307, 3306
FspI TGCGCA 1 cut(s) 169
GsaI CCCAGC 2 cut(s) 1563, 2436
GsuI CTGGAG 6 cut(s) 194, 245, 936, 1049, 3113, 3172
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 74, 476, 2501, 2522
HgaI GACGC 6 cut(s) 16, 74, 170, 384, 535, 3029
Hin1I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hin1II CATG 8 cut(s) 119, 430, 1084, 1526, 1571, 1684, 2581, 3115
HincII GTYRAC 3 cut(s) 943, 1529, 2235
HindII GTYRAC 3 cut(s) 943, 1529, 2235
HindIII AAGCTT 2 cut(s) 742, 1161
HphI GGTGA 8 cut(s) 25, 506, 535, 1373, 1374, 1634, 1809, 1894
Hpy166II GTNNAC 7 cut(s) 198, 514, 943, 1180, 1529, 1817, 2235
Hpy8I GTNNAC 7 cut(s) 198, 514, 943, 1180, 1529, 1817, 2235
HpyCH4III ACNGT 4 cut(s) 1357, 1474, 2915, 2930
HpyCH4IV ACGT 3 cut(s) 107, 675, 3208
HpySE526I ACGT 3 cut(s) 107, 675, 3208
Hsp92I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hsp92II CATG 8 cut(s) 119, 430, 1084, 1526, 1571, 1684, 2581, 3115
Kpn2I TCCGGA 1 cut(s) 483
Ksp22I TGATCA 3 cut(s) 1363, 1767, 2977
KspI CCGCGG 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 1159
LmnI GCTCC 9 cut(s) 90, 180, 213, 226, 283, 486, 549, 926, 3260
Lsp1109I GCAGC 9 cut(s) 32, 243, 397, 589, 1275, 1585, 1629, 2625, 3140
LweI GCATC 9 cut(s) 979, 1277, 1583, 1723, 1925, 1996, 2209, 2336, 2701
MaeI CTAG 4 cut(s) 1377, 1923, 2307, 3306
MaeII ACGT 3 cut(s) 107, 675, 3208
MbiI CCGCTC 2 cut(s) 183, 693
MfeI CAATTG 2 cut(s) 1549, 1763
MflI RGATCY 4 cut(s) 372, 1429, 2060, 3077
MhlI GDGCHC 5 cut(s) 417, 563, 1373, 1589, 3265
MlyI GAGTC 9 cut(s) 466, 547, 584, 1185, 2351, 2540, 2927, 3117, 3277
MmeI TCCRAC 3 cut(s) 590, 1996, 2239
Mph1103I ATGCAT 2 cut(s) 1918, 2011
MroI TCCGGA 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 3180
MseI TTAA 7 cut(s) 2012, 2294, 2441, 2571, 2765, 3012, 3063
MslI CAYNNNNRTG 2 cut(s) 1085, 1880
MspA1I CMGCKG 4 cut(s) 205, 602, 1620, 3091
MunI CAATTG 2 cut(s) 1549, 1763
MvaI CCWGG 5 cut(s) 25, 178, 1416, 2519, 2959
MvnI CGCG 8 cut(s) 52, 82, 98, 174, 188, 205, 506, 1064
NciI CCSGG 5 cut(s) 218, 350, 478, 2176, 3288
NlaIII CATG 8 cut(s) 119, 430, 1084, 1526, 1571, 1684, 2581, 3115
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 5 cut(s) 491, 523, 1882, 2100, 2930
NruI TCGCGA 1 cut(s) 506
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 2 cut(s) 1918, 2011
NspI RCATGY 2 cut(s) 1084, 1684
OliI CACNNNNGTG 1 cut(s) 1880
PaqCI CACCTGC 1 cut(s) 372
PciI ACATGT 1 cut(s) 1080
PciSI GCTCTTC 1 cut(s) 1159
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 648
PdmI GAANNNNTTC 1 cut(s) 3180
PflFI GACNNNGTC 2 cut(s) 106, 394
PleI GAGTC 9 cut(s) 465, 547, 584, 1184, 2351, 2540, 2927, 3116, 3277
PpsI GAGTC 9 cut(s) 465, 547, 584, 1184, 2351, 2540, 2927, 3116, 3277
PpuMI RGGWCCY 3 cut(s) 1129, 1203, 2862
PscI ACATGT 1 cut(s) 1080
PshAI GACNNNNGTC 1 cut(s) 1551
Psp124BI GAGCTC 1 cut(s) 417
Psp5II RGGWCCY 3 cut(s) 1129, 1203, 2862
Psp6I CCWGG 5 cut(s) 23, 176, 1414, 2517, 2957
PspEI GGTNACC 1 cut(s) 1882
PspFI CCCAGC 2 cut(s) 1559, 2432
PspGI CCWGG 5 cut(s) 23, 176, 1414, 2517, 2957
PspPI GGNCC 9 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2500, 2862
PspPPI RGGWCCY 3 cut(s) 1129, 1203, 2862
PstI CTGCAG 1 cut(s) 1603
PsuI RGATCY 4 cut(s) 372, 1429, 2060, 3077
PsyI GACNNNGTC 2 cut(s) 106, 394
PvuII CAGCTG 1 cut(s) 3091
RruI TCGCGA 1 cut(s) 506
RsaI GTAC 4 cut(s) 191, 197, 759, 2667
RsaNI GTAC 4 cut(s) 190, 196, 758, 2666
RseI CAYNNNNRTG 2 cut(s) 1085, 1880
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1159
SaqAI TTAA 7 cut(s) 2012, 2294, 2441, 2571, 2765, 3012, 3063
Sau96I GGNCC 9 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2500, 2862
ScaI AGTACT 1 cut(s) 2667
SchI GAGTC 9 cut(s) 466, 547, 584, 1185, 2351, 2540, 2927, 3117, 3277
SduI GDGCHC 5 cut(s) 417, 563, 1373, 1589, 3265
SfaNI GCATC 9 cut(s) 979, 1277, 1583, 1723, 1925, 1996, 2209, 2336, 2701
SfcI CTRYAG 3 cut(s) 1599, 2277, 2418
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 2 cut(s) 10, 520
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 8 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2862
SmiMI CAYNNNNRTG 2 cut(s) 1085, 1880
SmlI CTYRAG 2 cut(s) 404, 416
SmoI CTYRAG 2 cut(s) 404, 416
SpeI ACTAGT 1 cut(s) 1922
SspI AATATT 1 cut(s) 2704
SspMI CTAG 4 cut(s) 1377, 1923, 2307, 3306
SstI GAGCTC 1 cut(s) 417
TaaI ACNGT 4 cut(s) 1357, 1474, 2915, 2930
TaiI ACGT 3 cut(s) 110, 678, 3211
TaqI TCGA 8 cut(s) 269, 296, 411, 441, 639, 1170, 2360, 3018
TatI WGTACW 2 cut(s) 757, 2665
TauI GCSGC 8 cut(s) 50, 186, 205, 208, 314, 346, 532, 1067
Tru1I TTAA 7 cut(s) 2012, 2294, 2441, 2571, 2765, 3012, 3063
Tru9I TTAA 7 cut(s) 2012, 2294, 2441, 2571, 2765, 3012, 3063
TseFI GTSAC 5 cut(s) 491, 523, 1882, 2100, 2930
TseI GCWGC 9 cut(s) 20, 256, 385, 602, 1263, 1598, 1617, 2613, 3128
Tsp45I GTSAC 5 cut(s) 491, 523, 1882, 2100, 2930
TspGWI ACGGA 4 cut(s) 53, 223, 602, 693
Tth111I GACNNNGTC 2 cut(s) 106, 394
VpaK11BI GGWCC 8 cut(s) 250, 595, 1129, 1203, 1235, 1931, 2246, 2862
XapI RAATTY 4 cut(s) 1515, 2000, 2648, 3059
XceI RCATGY 2 cut(s) 1084, 1684
XmiI GTMKAC 1 cut(s) 1179
XmnI GAANNNNTTC 1 cut(s) 3180
XspI CTAG 4 cut(s) 1377, 1923, 2307, 3306
ZraI GACGTC 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 2667
Zsp2I ATGCAT 2 cut(s) 1918, 2011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.