Rh5AG457600
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010747
Physical Location & Seq
Forward (+)
78415976 .. 78421935
5960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG457600.1

Sequence Viewer

Length: 3333 bp
ATGCCGACGCCGGTAAGCGCAGCCAGGCAATGTTTGACGGAGGAGGCGGCGCGTGCGCTGGAAGACGCAGTGGCCGTGGCGCGTAGGAGGAGCCACGCGCAGACGACGTCGCTTCATGCGGTGTCGGCTCTGCTCTCCCTGCCGTCGTCTGCTCTCCGCGACGCCTGCGCACGCGCCAGGAGCGGCGCGTACCCGTACACGCCGCGGCTGCAGTTCCGGGCGCTGGAGCTGTCGGTCGGGGTGTCGCTGGACCGGCTGCCGTCGTCGTCGAAGGCGCAGGAGGAGCCGCCGGTGTCGAACTCGCTGATGGCGGCGATCAAGCGGTCGCAGGCGAACCAGAGGCGGCACCCGGAGAGCTTCCACCTGCACCAGATCCACAGCCAGCAGCAAACGGCGTCGCTTCTGAAGGTCGAGCTCAAGCACTTCATGCTGTCCATTCTCGACGACCCGATCGTGAGCCGGGTCTTCGGGGAGGCCGGGTTCCGGAGCTGTGACATAAAGCTCGCGATTGTTCACCCGCCGGTGACGCCGCAATCCCATCGGCTCCCTCGGACTCTGTGCCCGCCCATTTTCCTATGCAACTTAACGGACTCGGACCCAGCTGCCCGTCGGAGATTCCCTTTCCCGTTCTCGGGATTCGAGGAAAAGGACGAGAACTGCAGGAGAATCGGGGACGTGTTAGTTAGAAAGAGCGGGAAAAATCCGTTACTGATTGGCGTTTCCGCCACTGAAGCTCTCAAAAGCTTCGTTGAAGCTGTGCAGAAAGGCAGAGCCTCCCTTTTGCCTCCTGAGGTAACTAGCTTCAGTGTAGTCAGTATGGAAGAGGAGATTTCGGAGTTTGTGGTTGAGGGTGGGAGTGGGAGTAGTGAAGGGAAGATGGGGTTGAAGTTGAAGGAGGTGGGTCATTTGGCTCACCACTGTTCTGGAGACGGAGGAGCTGGTGTGATAGTCAACTTTGGGGAAATGAAGGCTTTGGTTGATGACGGGGTGTTGAGTGATGCTTTGAGCTTTGTGGTGGTTCAGTTGAAGGGGTTGCTGCAGATTCATAGTGGCAAGTTGTGGTTGATCGGCGCGGCCGGGAGTTACGACATGTATATGAAACTTCTGGCTCGGTTTCCGGCTATTGAGAAGGATTGGGACCTTCACCTCCTTCCCATTAGCTCTTCTAAAGCTTCAGTCGATGGAGTCTACTCTAAATCCAGCTTGATGGGGTCCTTTGTTCCATTTGGGGGATTCTTCTCTGGACCATCTGACTTTACAAATCCACTGAGCAGCACGAATCAATCTTTTATACGCTGCCATCAATGCACAGAGAAGTATGAGCAAGAAGTTGCTTCTATTCGGAAAGATGGATCAGCCATTACAGTTGGTGATCAGTGCTCAACTAGCTCACCTTCTTGGTTGCAAACGACTGAACTTGACCCTGGCAAGGGAGTGGATCTTGCAAAGACCAAAGCAGATAATACGACATTGAGTGAAACAGTATTGGGACTGCAAAAGAAATGGAATGATATCTGCCGAAAAATTCATCATGGTCAACCATTCCCTAAAATGGACAATTGTCAAGCTGGGTCTCATGTTGCATCTCCTGAGGGCTCTCATACTGCTACAGATAGAAGAGAAAGCAGCGGTGAAGATTCATCTATACAGGAAAGCCGAAGTGCAAAACATCACTGCCTTCCAATGGACATGCAAAAGAGTTTTCTGTCAAAACAGAACTTAGTAATGCAAGTAGCTTCTGATGCTGAGTATGCTGGTATGCAATCCAAGCAATTGATCAGAGATTCAAAGGGTCAGCAACTTGAACTGGGAAGCCCCTGCCGTTCACCTTTCCCTATCCCCACTATGAATCTTCCCACTGATCGCACATCATCCTCATCTATCACTTCGGTGACCACAGATTTAGGATTGGGAACTTTATATGCATCGACTAGTCAGGGACCAAGAAGTCCCAAGTTGCAAGATCATAGAGAGCGTCTCCGACACCTTTCTGGTTCCATTTCTGCTGAATTCGATACATTAAGTGAGAATAGTCCGCACCAAATAGCCCAGTCCTCTTCCTGTTCTGGATCTAATTTTGCTGGGCAGTTTGATCCAAGAGATTTCAAGTCACTTAGGAGAGTTCTCACAGCAAAGGTTTGCTGGCAAGATGAAGCAATATGCACCATTAGTGAAGCTATATCCCGGAACAGGTCTGGTGGTGGAAGGCATCGTCGTTCAAAAGGCAGAGGAGATATATGGTTGACTTTGATTGGACCTGACAGGGTTGGAAAGAAGAAAATTGCTATAGCACTTGCAGAGTTAATGTTTGGCACTAGGGAAAGCCTAATCTCTGTTGATATGGGGGAGAGGGGATGCCAATCTGACTCGATTTTTCAATGGGAATCCCAAGATGATTATGATGTGAAGTTTAGGGGTAAGACGGCTGTAGATTATGTTGCTGGGGAATTAAGCAGGAGACCCCATTCAGTTGTCTTCCTTGAAAATGTAGATAAGGCTGATTTTCTGGCCCAGAGTAACTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGACTCTCATGGCAGAGAAATCAGCATTAACAACATGATCTTTGTAACGACTTCAGCTTCCAAAAAGGGCAGTAAAAATCACTACTTGGAGAATGAACCCCTGAAATTCTCAGAGGAGATAGTACTTGGTGCCAAGAGGTGTCAAATGCAAATACTGAATATTGGGGATGCCAGCCAAACGAAGGGCGTGAATGTGAGGATTGCACCAAGAGAAGGAACCTTAAATTCATCTTCTGTAAATAAGAGAAAGCTGATTGACAGCAGTGCCTCCATAGAGGAAACCTCAGAGTTACAGAAACGGGGCAACAAGGCATCAAGGTCCTTTCTGGATTTGAATCTGCCTTTTGAAGAGATAGACGAGGGAATGAACTGTGGAGATTATGACAGTGACTCCATCTCCGAAAACTCAGAAGCCTGGATGGAAGACTTCCTTGATCAAGTTGATGAAGCAGTGGTTTTGAAGCCATTCAAGTTCGACGCTCTTGCTGAGAAATTAGTGAAGGAAATCAACCAAGAATTTAAGAAGGTCTTCGGATCCGAGTATCAGCTGGAGATTGACTTTGGAGTCATGGTTCAATTACTTGCAGCTTGTTGGTTATCAGACAAGAAGAAAGCAGTGGAGGAGTGGATTGAACAGGTTCTCTCCAGAAGTTTTGCTGAAGCACGTCAGAGGTATCGTCTCACTGCTTATTCTGTAATAAAATTGGTTGCCGGTGGAGCTCTTTCGGTGCAAGAGCAGACTCCCGGTGTTTGCCTTCCTGCTAGAATTAGTCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1110

Amino Acids

121.84

Weight (kDa)

6.99

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 116 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 216 - 391 9.9e-14 SMAX1 nucleotide binding domain
AAA_2 PF07724 749 - 878 1.5e-13 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1009 - 1088 1.3e-30 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 372
AasI GACNNNNNNGTC 2 cut(s) 1947, 3116
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 372
AccB1I GGYRCC 2 cut(s) 345, 2681
AccBSI CCGCTC 2 cut(s) 183, 693
AccI GTMKAC 1 cut(s) 1188
AccII CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
AccIII TCCGGA 1 cut(s) 483
AclWI GGATC 7 cut(s) 367, 1360, 1446, 2077, 2087, 3081, 3094
AcoI YGGCCR 2 cut(s) 72, 1074
AcsI RAATTY 5 cut(s) 1524, 2009, 2657, 2776, 3068
AcuI CTGAAG 6 cut(s) 425, 750, 787, 1158, 2589, 3231
AcyI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
AfaI GTAC 3 cut(s) 191, 197, 2676
AflIII ACRYGT 2 cut(s) 675, 1089
AhlI ACTAGT 1 cut(s) 1931
AjiI CACGTC 2 cut(s) 676, 3218
AjnI CCWGG 5 cut(s) 23, 176, 1423, 2526, 2966
AleI CACNNNNGTG 1 cut(s) 1889
AloI GAACNNNNNNTCC 4 cut(s) 464, 496, 3108, 3140
Alw21I GWGCWC 3 cut(s) 417, 1382, 3274
Alw26I GTCTC 5 cut(s) 921, 1578, 1982, 2452, 3236
AlwI GGATC 7 cut(s) 367, 1360, 1446, 2077, 2087, 3081, 3094
Ama87I CYCGRG 1 cut(s) 631
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 5 cut(s) 72, 474, 1074, 2508, 2529
ApoI RAATTY 5 cut(s) 1524, 2009, 2657, 2776, 3068
Asp700I GAANNNNTTC 3 cut(s) 3017, 3080, 3189
AspS9I GGNCC 9 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2509, 2871
AsuC2I CCSGG 7 cut(s) 218, 350, 461, 478, 1078, 2185, 3297
AsuHPI GGTGA 9 cut(s) 506, 535, 905, 1136, 1382, 1383, 1643, 1818, 1903
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 8 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2871
AxyI CCTNAGG 2 cut(s) 789, 1590
BaeGI GKGCMC 1 cut(s) 563
BamHI GGATCC 1 cut(s) 3086
BanI GGYRCC 2 cut(s) 345, 2681
BanII GRGCYC 3 cut(s) 417, 1598, 3274
BbsI GAAGAC 5 cut(s) 69, 457, 2467, 2982, 3073
Bbv12I GWGCWC 3 cut(s) 417, 1382, 3274
BceAI ACGGC 6 cut(s) 59, 127, 244, 408, 1806, 2439
BcgI CGANNNNNNTGC 6 cut(s) 521, 555, 649, 683, 3017, 3051
BciT130I CCWGG 5 cut(s) 25, 178, 1425, 2528, 2968
BclI TGATCA 3 cut(s) 1372, 1776, 2986
BcnI CCSGG 7 cut(s) 218, 350, 461, 478, 1078, 2185, 3297
BcoDI GTCTC 5 cut(s) 921, 1578, 1982, 2452, 3236
BcuI ACTAGT 1 cut(s) 1931
BfaI CTAG 5 cut(s) 798, 1386, 1932, 2316, 3315
BfmI CTRYAG 6 cut(s) 209, 658, 1037, 1608, 2286, 2427
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 372
BmcAI AGTACT 1 cut(s) 2676
Bme18I GGWCC 8 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2871
BmeT110I CYCGRG 1 cut(s) 631
BmgBI CACGTC 2 cut(s) 676, 3218
BmgT120I GGNCC 9 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2509, 2871
BmrI ACTGGG 2 cut(s) 1817, 2044
BmsI GCATC 8 cut(s) 988, 1592, 1732, 1934, 2218, 2345, 2710, 2873
BmuI ACTGGG 2 cut(s) 1817, 2044
BoxI GACNNNNGTC 1 cut(s) 1560
BpiI GAAGAC 5 cut(s) 69, 457, 2467, 2982, 3073
BplI GAGNNNNNCTC 4 cut(s) 1962, 1994, 2819, 2851
BpmI CTGGAG 4 cut(s) 245, 945, 3122, 3181
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 7 cut(s) 218, 350, 461, 478, 1078, 2185, 3297
BsaBI GATNNNNATC 2 cut(s) 2359, 2886
BsaHI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BsaI GGTCTC 2 cut(s) 1578, 2452
BsaJI CCNNGG 5 cut(s) 75, 203, 548, 1423, 2526
BsaWI WCCGGW 1 cut(s) 483
Bse118I RCCGGY 5 cut(s) 10, 252, 289, 520, 3263
Bse1I ACTGG 3 cut(s) 1812, 2050, 2545
Bse21I CCTNAGG 2 cut(s) 789, 1590
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 2 cut(s) 2359, 2886
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 5 cut(s) 25, 178, 1425, 2528, 2968
BseDI CCNNGG 5 cut(s) 75, 203, 548, 1423, 2526
BseGI GGATG 4 cut(s) 1871, 2360, 2725, 2976
BseJI GATNNNNATC 2 cut(s) 2359, 2886
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 8 cut(s) 780, 1259, 1581, 1737, 2676, 2850, 2973, 3030
BseNI ACTGG 3 cut(s) 1812, 2050, 2545
BseRI GAGGAG 8 cut(s) 56, 103, 296, 839, 948, 2244, 2681, 3188
BseSI GKGCMC 1 cut(s) 563
BseX3I CGGCCG 1 cut(s) 1074
BseYI CCCAGC 4 cut(s) 598, 1568, 2081, 2441
BsgI GTGCAG 2 cut(s) 350, 779
Bsh1236I CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
Bsh1285I CGRYCG 4 cut(s) 237, 326, 453, 1077
BshFI GGCC 5 cut(s) 74, 476, 1076, 2510, 2531
BshNI GGYRCC 2 cut(s) 345, 2681
BsiEI CGRYCG 4 cut(s) 237, 326, 453, 1077
BsiHKAI GWGCWC 3 cut(s) 417, 1382, 3274
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 6 cut(s) 686, 1151, 1503, 1935, 1953, 2509
BsmAI GTCTC 5 cut(s) 921, 1578, 1982, 2452, 3236
BsmBI CGTCTC 3 cut(s) 921, 1982, 3236
BsmFI GGGAC 6 cut(s) 686, 1151, 1503, 1935, 1953, 2509
BsnI GGCC 5 cut(s) 74, 476, 1076, 2510, 2531
Bso31I GGTCTC 2 cut(s) 1578, 2452
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 5 cut(s) 417, 563, 1382, 1598, 3274
Bsp13I TCCGGA 1 cut(s) 483
Bsp68I TCGCGA 1 cut(s) 506
BspANI GGCC 5 cut(s) 74, 476, 1076, 2510, 2531
BspCNI CTCAG 8 cut(s) 781, 1260, 1582, 1738, 2675, 2849, 2972, 3031
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
BspMAI CTGCAG 3 cut(s) 213, 662, 1041
BspMI ACCTGC 1 cut(s) 372
BspPI GGATC 7 cut(s) 367, 1360, 1446, 2077, 2087, 3081, 3094
BspQI GCTCTTC 1 cut(s) 1168
BspT107I GGYRCC 2 cut(s) 345, 2681
BspTNI GGTCTC 2 cut(s) 1578, 2452
BsrBI CCGCTC 2 cut(s) 183, 693
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 5 cut(s) 10, 252, 289, 520, 3263
BsrI ACTGG 3 cut(s) 1812, 2050, 2545
BssAI RCCGGY 5 cut(s) 10, 252, 289, 520, 3263
BssECI CCNNGG 5 cut(s) 75, 203, 548, 1423, 2526
BssNI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Bst2UI CCWGG 5 cut(s) 25, 178, 1425, 2528, 2968
Bst4CI ACNGT 5 cut(s) 920, 1366, 1483, 2924, 2939
Bst6I CTCTTC 5 cut(s) 816, 1168, 1612, 2062, 2895
BstACI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 9 cut(s) 54, 166, 172, 330, 383, 504, 563, 2144, 2725
BstDSI CCRYGG 2 cut(s) 75, 203
BstEII GGTNACC 1 cut(s) 1891
BstF5I GGATG 4 cut(s) 1871, 2360, 2725, 2976
BstFNI CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
BstH2I RGCGCY 1 cut(s) 224
BstMAI GTCTC 5 cut(s) 921, 1578, 1982, 2452, 3236
BstMCI CGRYCG 4 cut(s) 237, 326, 453, 1077
BstNI CCWGG 5 cut(s) 25, 178, 1425, 2528, 2968
BstNSI RCATGY 2 cut(s) 1093, 1693
BstPAI GACNNNNGTC 1 cut(s) 1560
BstPI GGTNACC 1 cut(s) 1891
BstSFI CTRYAG 6 cut(s) 209, 658, 1037, 1608, 2286, 2427
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
BstV2I GAAGAC 5 cut(s) 69, 457, 2467, 2982, 3073
BstX2I RGATCY 4 cut(s) 372, 1438, 2069, 3086
BstXI CCANNNNNNTGG 2 cut(s) 923, 1207
BstYI RGATCY 4 cut(s) 372, 1438, 2069, 3086
BstZI CGGCCG 1 cut(s) 1074
Bsu36I CCTNAGG 2 cut(s) 789, 1590
BsuRI GGCC 5 cut(s) 74, 476, 1076, 2510, 2531
BtgI CCRYGG 2 cut(s) 75, 203
BtrI CACGTC 2 cut(s) 676, 3218
BtsCI GGATG 4 cut(s) 1871, 2360, 2725, 2976
BtsI GCAGTG 6 cut(s) 75, 1672, 2821, 3009, 3174, 3234
BtuMI TCGCGA 1 cut(s) 506
BveI ACCTGC 1 cut(s) 372
Cac8I GCNNGC 9 cut(s) 54, 166, 172, 330, 383, 504, 563, 2144, 2725
Cfr10I RCCGGY 5 cut(s) 10, 252, 289, 520, 3263
Cfr13I GGNCC 9 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2509, 2871
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1964, 3038
Csp6I GTAC 3 cut(s) 190, 196, 2675
CviAII CATG 9 cut(s) 116, 427, 1090, 1532, 1577, 1690, 2561, 2587, 3121
CviQI GTAC 3 cut(s) 190, 196, 2675
DrdI GACNNNNNNGTC 2 cut(s) 1947, 3116
DseDI GACNNNNNNGTC 2 cut(s) 1947, 3116
EaeI YGGCCR 2 cut(s) 72, 1074
EagI CGGCCG 1 cut(s) 1074
Eam1104I CTCTTC 5 cut(s) 816, 1168, 1612, 2062, 2895
EarI CTCTTC 5 cut(s) 816, 1168, 1612, 2062, 2895
EciI GGCGGA 1 cut(s) 712
Ecl136II GAGCTC 2 cut(s) 415, 3272
EclXI CGGCCG 1 cut(s) 1074
Eco24I GRGCYC 3 cut(s) 417, 1598, 3274
Eco31I GGTCTC 2 cut(s) 1578, 2452
Eco32I GATATC 1 cut(s) 1513
Eco47I GGWCC 8 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2871
Eco52I CGGCCG 1 cut(s) 1074
Eco53kI GAGCTC 2 cut(s) 415, 3272
Eco57I CTGAAG 6 cut(s) 425, 750, 787, 1158, 2589, 3231
Eco81I CCTNAGG 2 cut(s) 789, 1590
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 1891
EcoICRI GAGCTC 2 cut(s) 415, 3272
EcoO109I RGGNCCY 3 cut(s) 1138, 1212, 2871
EcoO65I GGTNACC 1 cut(s) 1891
EcoRI GAATTC 1 cut(s) 2009
EcoRII CCWGG 5 cut(s) 23, 176, 1423, 2526, 2966
EcoRV GATATC 1 cut(s) 1513
EcoT22I ATGCAT 1 cut(s) 1927
EcoT38I GRGCYC 3 cut(s) 417, 1598, 3274
Esp3I CGTCTC 3 cut(s) 921, 1982, 3236
FaeI CATG 9 cut(s) 119, 430, 1093, 1535, 1580, 1693, 2564, 2590, 3124
FalI AAGNNNNNCTT 4 cut(s) 2967, 2999, 3065, 3097
FaqI GGGAC 6 cut(s) 686, 1151, 1503, 1935, 1953, 2509
FatI CATG 9 cut(s) 115, 426, 1089, 1531, 1576, 1689, 2560, 2586, 3120
FauI CCCGC 3 cut(s) 525, 570, 686
FbaI TGATCA 3 cut(s) 1372, 1776, 2986
FblI GTMKAC 1 cut(s) 1188
FokI GGATG 4 cut(s) 1858, 2367, 2732, 2983
FriOI GRGCYC 3 cut(s) 417, 1598, 3274
FspBI CTAG 5 cut(s) 798, 1386, 1932, 2316, 3315
FspI TGCGCA 1 cut(s) 169
GsaI CCCAGC 4 cut(s) 602, 1572, 2085, 2445
GsuI CTGGAG 4 cut(s) 245, 945, 3122, 3181
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 5 cut(s) 74, 476, 1076, 2510, 2531
HgaI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1964, 3038
Hin1I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hin1II CATG 9 cut(s) 119, 430, 1093, 1535, 1580, 1693, 2564, 2590, 3124
HincII GTYRAC 3 cut(s) 952, 1538, 2244
HindII GTYRAC 3 cut(s) 952, 1538, 2244
HindIII AAGCTT 2 cut(s) 742, 1170
HphI GGTGA 9 cut(s) 506, 535, 905, 1136, 1382, 1383, 1643, 1818, 1903
Hpy166II GTNNAC 7 cut(s) 198, 514, 952, 1189, 1538, 1826, 2244
Hpy8I GTNNAC 7 cut(s) 198, 514, 952, 1189, 1538, 1826, 2244
HpyCH4III ACNGT 5 cut(s) 920, 1366, 1483, 2924, 2939
HpyCH4IV ACGT 3 cut(s) 107, 675, 3217
HpySE526I ACGT 3 cut(s) 107, 675, 3217
Hsp92I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hsp92II CATG 9 cut(s) 119, 430, 1093, 1535, 1580, 1693, 2564, 2590, 3124
Kpn2I TCCGGA 1 cut(s) 483
Ksp22I TGATCA 3 cut(s) 1372, 1776, 2986
KspI CCGCGG 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 1168
LmnI GCTCC 8 cut(s) 90, 180, 226, 283, 486, 549, 935, 3269
LweI GCATC 8 cut(s) 988, 1592, 1732, 1934, 2218, 2345, 2710, 2873
MaeI CTAG 5 cut(s) 798, 1386, 1932, 2316, 3315
MaeII ACGT 3 cut(s) 107, 675, 3217
MbiI CCGCTC 2 cut(s) 183, 693
MfeI CAATTG 2 cut(s) 1558, 1772
MflI RGATCY 4 cut(s) 372, 1438, 2069, 3086
MhlI GDGCHC 5 cut(s) 417, 563, 1382, 1598, 3274
MlyI GAGTC 8 cut(s) 547, 584, 1194, 2360, 2549, 2936, 3126, 3286
MmeI TCCRAC 3 cut(s) 590, 2005, 2248
Mph1103I ATGCAT 1 cut(s) 1927
MroI TCCGGA 1 cut(s) 483
MroXI GAANNNNTTC 3 cut(s) 3017, 3080, 3189
MseI TTAA 7 cut(s) 584, 2021, 2303, 2450, 2580, 2774, 3072
MslI CAYNNNNRTG 2 cut(s) 1094, 1889
MspA1I CMGCKG 4 cut(s) 205, 602, 1629, 3100
MunI CAATTG 2 cut(s) 1558, 1772
MvaI CCWGG 5 cut(s) 25, 178, 1425, 2528, 2968
MvnI CGCG 9 cut(s) 52, 82, 98, 159, 174, 188, 205, 506, 1073
NciI CCSGG 7 cut(s) 218, 350, 461, 478, 1078, 2185, 3297
NlaIII CATG 9 cut(s) 119, 430, 1093, 1535, 1580, 1693, 2564, 2590, 3124
NmuCI GTSAC 5 cut(s) 491, 523, 1891, 2109, 2939
NruI TCGCGA 1 cut(s) 506
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1927
NspI RCATGY 2 cut(s) 1093, 1693
OliI CACNNNNGTG 1 cut(s) 1889
PaqCI CACCTGC 1 cut(s) 372
PciI ACATGT 1 cut(s) 1089
PciSI GCTCTTC 1 cut(s) 1168
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 450
PdmI GAANNNNTTC 3 cut(s) 3017, 3080, 3189
PflFI GACNNNGTC 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 2183
Ple19I CGATCG 1 cut(s) 453
PleI GAGTC 8 cut(s) 547, 584, 1193, 2360, 2549, 2936, 3125, 3286
PpsI GAGTC 8 cut(s) 547, 584, 1193, 2360, 2549, 2936, 3125, 3286
PpuMI RGGWCCY 3 cut(s) 1138, 1212, 2871
PscI ACATGT 1 cut(s) 1089
PshAI GACNNNNGTC 1 cut(s) 1560
Psp124BI GAGCTC 2 cut(s) 417, 3274
Psp5II RGGWCCY 3 cut(s) 1138, 1212, 2871
Psp6I CCWGG 5 cut(s) 23, 176, 1423, 2526, 2966
PspEI GGTNACC 1 cut(s) 1891
PspFI CCCAGC 4 cut(s) 598, 1568, 2081, 2441
PspGI CCWGG 5 cut(s) 23, 176, 1423, 2526, 2966
PspPI GGNCC 9 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2509, 2871
PspPPI RGGWCCY 3 cut(s) 1138, 1212, 2871
PstI CTGCAG 3 cut(s) 213, 662, 1041
PsuI RGATCY 4 cut(s) 372, 1438, 2069, 3086
PsyI GACNNNGTC 1 cut(s) 106
PvuI CGATCG 1 cut(s) 453
PvuII CAGCTG 2 cut(s) 602, 3100
RruI TCGCGA 1 cut(s) 506
RsaI GTAC 3 cut(s) 191, 197, 2676
RsaNI GTAC 3 cut(s) 190, 196, 2675
RseI CAYNNNNRTG 2 cut(s) 1094, 1889
SacI GAGCTC 2 cut(s) 417, 3274
SacII CCGCGG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1168
SaqAI TTAA 7 cut(s) 584, 2021, 2303, 2450, 2580, 2774, 3072
Sau96I GGNCC 9 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2509, 2871
ScaI AGTACT 1 cut(s) 2676
SchI GAGTC 8 cut(s) 547, 584, 1194, 2360, 2549, 2936, 3126, 3286
SduI GDGCHC 5 cut(s) 417, 563, 1382, 1598, 3274
SfaNI GCATC 8 cut(s) 988, 1592, 1732, 1934, 2218, 2345, 2710, 2873
SfcI CTRYAG 6 cut(s) 209, 658, 1037, 1608, 2286, 2427
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 2 cut(s) 289, 520
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 8 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2871
SmiMI CAYNNNNRTG 2 cut(s) 1094, 1889
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 1931
SspI AATATT 1 cut(s) 2713
SspMI CTAG 5 cut(s) 798, 1386, 1932, 2316, 3315
SstI GAGCTC 2 cut(s) 417, 3274
TaaI ACNGT 5 cut(s) 920, 1366, 1483, 2924, 2939
TaiI ACGT 3 cut(s) 110, 678, 3220
TaqII GACCGA 1 cut(s) 223
TatI WGTACW 1 cut(s) 2674
TauI GCSGC 9 cut(s) 50, 186, 205, 208, 289, 314, 346, 532, 1076
Tru1I TTAA 7 cut(s) 584, 2021, 2303, 2450, 2580, 2774, 3072
Tru9I TTAA 7 cut(s) 584, 2021, 2303, 2450, 2580, 2774, 3072
TseFI GTSAC 5 cut(s) 491, 523, 1891, 2109, 2939
Tsp45I GTSAC 5 cut(s) 491, 523, 1891, 2109, 2939
TspGWI ACGGA 4 cut(s) 53, 602, 693, 945
Tth111I GACNNNGTC 1 cut(s) 106
VpaK11BI GGWCC 8 cut(s) 250, 595, 1138, 1212, 1244, 1940, 2255, 2871
XapI RAATTY 5 cut(s) 1524, 2009, 2657, 2776, 3068
XceI RCATGY 2 cut(s) 1093, 1693
XmiI GTMKAC 1 cut(s) 1188
XmnI GAANNNNTTC 3 cut(s) 3017, 3080, 3189
XspI CTAG 5 cut(s) 798, 1386, 1932, 2316, 3315
ZraI GACGTC 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 2676
Zsp2I ATGCAT 1 cut(s) 1927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.