Rw5G042720
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010747
Physical Location & Seq
Forward (+)
74699119 .. 74704204
5086 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042720.1

Sequence Viewer

Length: 3261 bp
ATGCCGACGCCGGTAAGCGCAGCCAGGCAATGTTTGACGGAGGAGGCGGCGCGTGCGCTGGACGACGCAGTGGCCGTGGCGCGTAGGAGGAGCCACGCGCAGACGACGTCGCTTCATGCGGTGTCGGCTCTGCTCTCCCTGCCGTCGTCCGCTCTCCGCGACGCCTGCGCACGCGCCAGGAGCGGCGCGTACCCGTACACGCCGCGGCTGCAGTTCCGGGCGCTGGAGCTGTCGGTCGGGGTGTCGCTGGACCGGCTGCCGTCGTCGTCGAAGGCGCAGGAGGAGCCGCCGGTGTCGAACTCGCTGATGGCGGCGATCAAGCGGTCGCAGGCGAACCAGAGGCGGCACCCGGAGAGCTTCCACCTGCACCAGATCCACAGCCAGCAGCAGACGGCGTCGCTTCTCAAGGTCGAGCTCAAGCACTTCATGCTGTCCATTCTCGACGACCCGATCGTGAGCCGGGTCTTCGGGGAGGCCGGGTTCCGGAGCTGTGACATAAAGCTCGCGATTGTTCACCCGCCGGTGACGCCGCAATCCCATCGGCTCCCTCGGACTCTGTGCCCGCCCATTTTCCTATGCAACCTAACGGACTCGGACCCAGCTGCCCGTCGGAGATTCCCTTTCCCGTTATCGGGATTCGAGGAAAAGGACGAGAACTGCAGGAGAATCGGGGACGTGTTAGTTAGAAAGAGCGGGAAAAATCCGTTACTGATTGGCGTTTCCGCCACCGAAGCTCTCAAAAGCTTCATTGAAGCTGTGCAGAAAGGCAGAGCCTCCCTTTTGCCTCCTGAGGTAACTAGCTTCAGTGTAGTTAGTATGGAAGAGGAGATTTCGGAGTTTGTGGTTGAGGGTGGGAGTAGTGAAGGGAAGATGGGGTTGAAGTTGAAGGAGGTGGGTCATTTGGCTCACCAGTGTTCTGGAGACGGAGGAGCTGGTGTGATAGTCAACTTTGGGGAAATGAAGGCTTTGGTTGATGACGGGGTGTTGAGTGATGCTTTGAGCTTTGTGGTGGTTCAGTTGAAGGGGTTGCTGGAGATTCATAGTGGCAAGTTGTGGTTGATCGGCGCCGCCGGGAGTTACGACATGTATATGAAACTTCTCGCTCGGTTTCCGGCTATTGAGAAGGATTGGGACCTTCACCTCCTTCCCATTAGCTCTTCTAAAGCTTCAGTCGATGGAGTCTACTCTAAATCCAGCTTGATGGGGTCCTTTGTTCCATTTGGGGGATTCTTCTCTGGACCATCTGACTTTACAAATCCACTGAGCAGCACGAATCAATCTTTTATACGCTGCCATCAATGCACAGAGAAGTACGAGCAAGAAGTTGCTTCTATTCGGAAAGATGGATCGGCCATTACAGTTGGTGATCAGTGCTCAACTAGCTCACCTTCTTGGTTGCAAACGACTGAACTTGATCCTGGCAAGGGACTGGATCTTGCAAAGACCAAAGCAGATAATACGACATTGAGTGAAACAGTATTGGGACTGCAAAAGAAATGGAATGATATCTGCCGAAAAATTCATCATGGTCAACCATTCCCTAAAATGGACAATTGTCAAGCTGGGTCTCATGTTGCATCTCCTGAGGGCTCTCATACTGCTACAGATAGAAGAGAAAGCAGCGGTGAAGATTCATCTATACAGGAAAGCCGAAGTGCAAAACATCACTGCCTTCCAATGGACATGCAAAAGAGTTTTCTGTCAAAACAGAACTTAGTAATGCAAGTAGCTTCTGATGCTGAGTATGCTGGTATGCAATCCAAGCAATTGATCAGAGATTCAAAGGGTCAGCAACTTGAACTGGGAAGCCCCTGCCGTTCACCTTTCCCTATCCCCACTATGAATCTTCCTGCTGATCGCACATCATCCTCATCTATCACTTCGGTGACCACAGATTTGGGATTGGGAACCTTATATGCATCAACTAGTCAGGGACCAAGAAGTCCCAAGTTGCAAGATCATAGAGAGCGTCTCCGACACCTTTCTGGTTCCATTTCTGCTGAATTCGATACATTAAGTGAGAATAGTCCGCACCAAATAGCCCAGTCCTCTTCCTGCTCTGGATCTAATTTTGCTGGGCAGTTTGATCCAAGAGATTTCAAGTCACTTAGGAGAGTTCTCACAGCAAAGGTTTGCTGGCAAGATGAAGCAATATGCACCATTAGTGAAGCTATATCCCGGAGCAGGTCTGGTGGTGGAAGGCATCGTCGTTCAAAAGGCAGAGGAGATATATGGTTGACTTTGATTGGACCTGACAGGGTTGGAAAGAAGAAAATTGCTATAGCACTTGCAGAGTTAATGTTTGGCACTAGGGAAAGCCTAATCTCTGTTGATATGGGGGAGAGGGGATGCCAATCTGACTCTATTTTTCAATGGGAATCCCAAGATGATTATGATGTGAAGTTTAGGGGTAAGACGGCTGTAGATTATGTTGCTGGGGAATTAAGCAGGAGACCCCATTCAGTTGTCTTCCTTGAAAATGTAGATAAGGCTGATTTTCTGGCCCAGAGTAACTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGACTCTCATGGCAGAGAAATCAGCGTTAACAACATGATCTTTGTAACGACTTCAGCTACCAAAAAGGGCAGCAAAAATCACTACTTGGAGAATGAACCCCTGAAATTCTCAGAGGAGATAGTACTTGGTGCCAAGAGCCAAACGAAGGGCGTGAATGTGAGGATTGCACCAAGAGAAGGAACCTTAAATTCATCTTCTTTAAATAAGAGAAAGCTGATTGACAGCAGTGCCTCCATAGAGGAAACCTCAGAGTCCTTTCTGGATTTGAATCTGCCTTTTGAAGAGATAGACGAGGGAATGAACTGTGGAGATTATGACAGTGACTCCATCTCAGAAAACTCAGAAGCCTGGATGGAAGACTTCCTTGATCAAGTTGATGAAGCAGTGGTTTTGAAGCCATTCAAGTTCGACGCTCTTGCTGAGAAATTAGTGAAGGAAATCAACCAAGAATTTAAGAAGGTCTTCGGATCTGAGTATCAGCTGGAGATTGACTTTGGTGTCATGGTTCAATTACTTGCAGCTTGTTGGTTATCAGACAAGAAGAAAGGAGTGGAGGAGTGGATTGAACAGGTTCTCTCCAGAAGTTTTGCTGAAGCACGACAGAGGTATCGTCTCACTGCTCATTCTGTAATAAAATTGGTTGCCGGTGGAGCACTTTCGGTGCAAGAGCAGACTCCCGGTGTTTGCCTTCCTGCTAGAATTAGTCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1086

Amino Acids

119.1

Weight (kDa)

6.5

Isoelectric Point (pI)

53.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 116 1.1e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 216 - 389 1.1e-14 SMAX1 nucleotide binding domain
AAA_2 PF07724 747 - 876 6.8e-14 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 985 - 1064 3.4e-31 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 372
AasI GACNNNNNNGTC 2 cut(s) 1941, 3044
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 2 cut(s) 372, 2175
AccB1I GGYRCC 3 cut(s) 345, 1064, 2675
AccBSI CCGCTC 3 cut(s) 152, 183, 693
AccI GTMKAC 1 cut(s) 1182
AccII CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
AccIII TCCGGA 1 cut(s) 483
AclWI GGATC 7 cut(s) 367, 1354, 1409, 1440, 2071, 2081, 3022
AcoI YGGCCR 2 cut(s) 72, 1350
AcsI RAATTY 5 cut(s) 1518, 2003, 2651, 2734, 2996
AcuI CTGAAG 4 cut(s) 787, 1152, 2583, 3159
AcyI GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
AfaI GTAC 4 cut(s) 191, 197, 1313, 2670
AflIII ACRYGT 2 cut(s) 675, 1083
AhlI ACTAGT 1 cut(s) 1925
AjiI CACGTC 1 cut(s) 676
AjnI CCWGG 5 cut(s) 23, 176, 1417, 2520, 2894
AleI CACNNNNGTG 1 cut(s) 1883
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
Alw21I GWGCWC 3 cut(s) 417, 1376, 3202
Alw26I GTCTC 5 cut(s) 915, 1572, 1976, 2446, 3164
AlwI GGATC 7 cut(s) 367, 1354, 1409, 1440, 2071, 2081, 3022
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 5 cut(s) 72, 474, 1350, 2502, 2523
ApoI RAATTY 5 cut(s) 1518, 2003, 2651, 2734, 2996
ArsI GACNNNNNNTTYG 2 cut(s) 1879, 1911
Asp700I GAANNNNTTC 3 cut(s) 2945, 3008, 3117
AspS9I GGNCC 8 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249, 2503
AsuC2I CCSGG 7 cut(s) 218, 350, 461, 478, 1072, 2179, 3225
AsuHPI GGTGA 9 cut(s) 506, 535, 899, 1130, 1376, 1377, 1637, 1812, 1897
AvaII GGWCC 7 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249
AxyI CCTNAGG 2 cut(s) 789, 1584
BaeGI GKGCMC 1 cut(s) 563
BaeI ACNNNNGTAYC 2 cut(s) 3137, 3170
BanI GGYRCC 3 cut(s) 345, 1064, 2675
BanII GRGCYC 2 cut(s) 417, 1592
BbsI GAAGAC 4 cut(s) 457, 2461, 2910, 3001
Bbv12I GWGCWC 3 cut(s) 417, 1376, 3202
BceAI ACGGC 6 cut(s) 59, 127, 244, 408, 1800, 2433
BcgI CGANNNNNNTGC 6 cut(s) 521, 555, 649, 683, 2945, 2979
BciT130I CCWGG 5 cut(s) 25, 178, 1419, 2522, 2896
BclI TGATCA 3 cut(s) 1366, 1770, 2914
BcnI CCSGG 7 cut(s) 218, 350, 461, 478, 1072, 2179, 3225
BcoDI GTCTC 5 cut(s) 915, 1572, 1976, 2446, 3164
BcuI ACTAGT 1 cut(s) 1925
BfaI CTAG 5 cut(s) 798, 1380, 1926, 2310, 3243
BfmI CTRYAG 5 cut(s) 209, 658, 1602, 2280, 2421
BfoI RGCGCY 2 cut(s) 224, 1068
BfuAI ACCTGC 2 cut(s) 372, 2175
BmcAI AGTACT 1 cut(s) 2670
Bme18I GGWCC 7 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249
BmgBI CACGTC 1 cut(s) 676
BmgT120I GGNCC 8 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249, 2503
BmrI ACTGGG 2 cut(s) 1811, 2038
BmsI GCATC 6 cut(s) 982, 1586, 1726, 1928, 2212, 2339
BmuI ACTGGG 2 cut(s) 1811, 2038
BoxI GACNNNNGTC 1 cut(s) 1554
BpiI GAAGAC 4 cut(s) 457, 2461, 2910, 3001
BplI GAGNNNNNCTC 4 cut(s) 1956, 1988, 2777, 2809
BpmI CTGGAG 5 cut(s) 245, 939, 1052, 3050, 3109
BpuEI CTTGAG 2 cut(s) 389, 401
BpuMI CCSGG 7 cut(s) 218, 350, 461, 478, 1072, 2179, 3225
BsaBI GATNNNNATC 2 cut(s) 2353, 2814
BsaHI GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
BsaI GGTCTC 2 cut(s) 1572, 2446
BsaJI CCNNGG 4 cut(s) 75, 203, 548, 2520
BsaWI WCCGGW 1 cut(s) 483
BsaXI ACNNNNNCTCC 6 cut(s) 464, 494, 921, 951, 2855, 2885
Bse118I RCCGGY 5 cut(s) 10, 252, 289, 520, 3191
Bse1I ACTGG 5 cut(s) 910, 1434, 1806, 2044, 2539
Bse21I CCTNAGG 2 cut(s) 789, 1584
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 2 cut(s) 2353, 2814
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 5 cut(s) 25, 178, 1419, 2522, 2896
BseDI CCNNGG 4 cut(s) 75, 203, 548, 2520
BseGI GGATG 3 cut(s) 1865, 2354, 2904
BseJI GATNNNNATC 2 cut(s) 2353, 2814
BseMI GCAATG 1 cut(s) 35
BseNI ACTGG 5 cut(s) 910, 1434, 1806, 2044, 2539
BseRI GAGGAG 8 cut(s) 56, 103, 296, 839, 942, 2238, 2675, 3116
BseSI GKGCMC 1 cut(s) 563
BseYI CCCAGC 4 cut(s) 598, 1562, 2075, 2435
BsgI GTGCAG 2 cut(s) 350, 779
Bsh1236I CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
Bsh1285I CGRYCG 3 cut(s) 237, 326, 453
BshFI GGCC 5 cut(s) 74, 476, 1352, 2504, 2525
BshNI GGYRCC 3 cut(s) 345, 1064, 2675
BsiEI CGRYCG 3 cut(s) 237, 326, 453
BsiHKAI GWGCWC 3 cut(s) 417, 1376, 3202
BslFI GGGAC 7 cut(s) 686, 1145, 1440, 1497, 1929, 1947, 2503
BsmAI GTCTC 5 cut(s) 915, 1572, 1976, 2446, 3164
BsmBI CGTCTC 3 cut(s) 915, 1976, 3164
BsmFI GGGAC 7 cut(s) 686, 1145, 1440, 1497, 1929, 1947, 2503
BsnI GGCC 5 cut(s) 74, 476, 1352, 2504, 2525
Bso31I GGTCTC 2 cut(s) 1572, 2446
Bsp1286I GDGCHC 5 cut(s) 417, 563, 1376, 1592, 3202
Bsp13I TCCGGA 1 cut(s) 483
Bsp68I TCGCGA 1 cut(s) 506
BspANI GGCC 5 cut(s) 74, 476, 1352, 2504, 2525
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
BspMAI CTGCAG 2 cut(s) 213, 662
BspMI ACCTGC 2 cut(s) 372, 2175
BspPI GGATC 7 cut(s) 367, 1354, 1409, 1440, 2071, 2081, 3022
BspQI GCTCTTC 1 cut(s) 1162
BspT107I GGYRCC 3 cut(s) 345, 1064, 2675
BspTNI GGTCTC 2 cut(s) 1572, 2446
BsrBI CCGCTC 3 cut(s) 152, 183, 693
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 5 cut(s) 10, 252, 289, 520, 3191
BsrI ACTGG 5 cut(s) 910, 1434, 1806, 2044, 2539
BssAI RCCGGY 5 cut(s) 10, 252, 289, 520, 3191
BssECI CCNNGG 4 cut(s) 75, 203, 548, 2520
BssNI GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
Bst2UI CCWGG 5 cut(s) 25, 178, 1419, 2522, 2896
Bst4CI ACNGT 4 cut(s) 1360, 1477, 2852, 2867
Bst6I CTCTTC 5 cut(s) 816, 1162, 1606, 2056, 2823
BstACI GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
BstAPI GCANNNNNTGC 1 cut(s) 427
BstC8I GCNNGC 8 cut(s) 54, 166, 172, 330, 383, 504, 563, 2138
BstDSI CCRYGG 2 cut(s) 75, 203
BstEII GGTNACC 1 cut(s) 1885
BstF5I GGATG 3 cut(s) 1865, 2354, 2904
BstFNI CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
BstH2I RGCGCY 2 cut(s) 224, 1068
BstMAI GTCTC 5 cut(s) 915, 1572, 1976, 2446, 3164
BstMCI CGRYCG 3 cut(s) 237, 326, 453
BstNI CCWGG 5 cut(s) 25, 178, 1419, 2522, 2896
BstNSI RCATGY 2 cut(s) 1087, 1687
BstPAI GACNNNNGTC 1 cut(s) 1554
BstPI GGTNACC 1 cut(s) 1885
BstSFI CTRYAG 5 cut(s) 209, 658, 1602, 2280, 2421
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
BstV2I GAAGAC 4 cut(s) 457, 2461, 2910, 3001
BstX2I RGATCY 4 cut(s) 372, 1432, 2063, 3014
BstXI CCANNNNNNTGG 3 cut(s) 917, 1201, 1897
BstYI RGATCY 4 cut(s) 372, 1432, 2063, 3014
Bsu36I CCTNAGG 2 cut(s) 789, 1584
BsuRI GGCC 5 cut(s) 74, 476, 1352, 2504, 2525
BtgI CCRYGG 2 cut(s) 75, 203
BtrI CACGTC 1 cut(s) 676
BtsCI GGATG 3 cut(s) 1865, 2354, 2904
BtsI GCAGTG 5 cut(s) 75, 1666, 2779, 2937, 3162
BtuMI TCGCGA 1 cut(s) 506
BveI ACCTGC 2 cut(s) 372, 2175
Cac8I GCNNGC 8 cut(s) 54, 166, 172, 330, 383, 504, 563, 2138
Cfr10I RCCGGY 5 cut(s) 10, 252, 289, 520, 3191
Cfr13I GGNCC 8 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249, 2503
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1958, 2966
Csp6I GTAC 4 cut(s) 190, 196, 1312, 2669
CviAII CATG 9 cut(s) 116, 427, 1084, 1526, 1571, 1684, 2555, 2581, 3049
CviQI GTAC 4 cut(s) 190, 196, 1312, 2669
DinI GGCGCC 1 cut(s) 1066
DraI TTTAAA 1 cut(s) 2748
DrdI GACNNNNNNGTC 2 cut(s) 1941, 3044
DseDI GACNNNNNNGTC 2 cut(s) 1941, 3044
EaeI YGGCCR 2 cut(s) 72, 1350
Eam1104I CTCTTC 5 cut(s) 816, 1162, 1606, 2056, 2823
EarI CTCTTC 5 cut(s) 816, 1162, 1606, 2056, 2823
EciI GGCGGA 1 cut(s) 712
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 2 cut(s) 417, 1592
Eco31I GGTCTC 2 cut(s) 1572, 2446
Eco32I GATATC 1 cut(s) 1507
Eco47I GGWCC 7 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 4 cut(s) 787, 1152, 2583, 3159
Eco81I CCTNAGG 2 cut(s) 789, 1584
Eco91I GGTNACC 1 cut(s) 1885
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 2 cut(s) 1132, 1206
EcoO65I GGTNACC 1 cut(s) 1885
EcoRI GAATTC 1 cut(s) 2003
EcoRII CCWGG 5 cut(s) 23, 176, 1417, 2520, 2894
EcoRV GATATC 1 cut(s) 1507
EcoT22I ATGCAT 1 cut(s) 1921
EcoT38I GRGCYC 2 cut(s) 417, 1592
EgeI GGCGCC 1 cut(s) 1066
EheI GGCGCC 1 cut(s) 1066
Esp3I CGTCTC 3 cut(s) 915, 1976, 3164
FaeI CATG 9 cut(s) 119, 430, 1087, 1529, 1574, 1687, 2558, 2584, 3052
FalI AAGNNNNNCTT 4 cut(s) 2895, 2927, 2993, 3025
FaqI GGGAC 7 cut(s) 686, 1145, 1440, 1497, 1929, 1947, 2503
FatI CATG 9 cut(s) 115, 426, 1083, 1525, 1570, 1683, 2554, 2580, 3048
FauI CCCGC 3 cut(s) 525, 570, 686
FbaI TGATCA 3 cut(s) 1366, 1770, 2914
FblI GTMKAC 1 cut(s) 1182
FokI GGATG 3 cut(s) 1852, 2361, 2911
FriOI GRGCYC 2 cut(s) 417, 1592
FspBI CTAG 5 cut(s) 798, 1380, 1926, 2310, 3243
FspI TGCGCA 1 cut(s) 169
GsaI CCCAGC 4 cut(s) 602, 1566, 2079, 2439
GsuI CTGGAG 5 cut(s) 245, 939, 1052, 3050, 3109
HaeII RGCGCY 2 cut(s) 224, 1068
HaeIII GGCC 5 cut(s) 74, 476, 1352, 2504, 2525
HgaI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1958, 2966
Hin1I GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
Hin1II CATG 9 cut(s) 119, 430, 1087, 1529, 1574, 1687, 2558, 2584, 3052
HincII GTYRAC 4 cut(s) 946, 1532, 2238, 2575
HindII GTYRAC 4 cut(s) 946, 1532, 2238, 2575
HindIII AAGCTT 2 cut(s) 742, 1164
HpaI GTTAAC 1 cut(s) 2575
HphI GGTGA 9 cut(s) 506, 535, 899, 1130, 1376, 1377, 1637, 1812, 1897
Hpy166II GTNNAC 8 cut(s) 198, 514, 946, 1183, 1532, 1820, 2238, 2575
Hpy8I GTNNAC 8 cut(s) 198, 514, 946, 1183, 1532, 1820, 2238, 2575
HpyCH4III ACNGT 4 cut(s) 1360, 1477, 2852, 2867
HpyCH4IV ACGT 2 cut(s) 107, 675
HpySE526I ACGT 2 cut(s) 107, 675
Hsp92I GRCGYC 6 cut(s) 8, 107, 162, 395, 527, 1065
Hsp92II CATG 9 cut(s) 119, 430, 1087, 1529, 1574, 1687, 2558, 2584, 3052
KasI GGCGCC 1 cut(s) 1064
Kpn2I TCCGGA 1 cut(s) 483
Ksp22I TGATCA 3 cut(s) 1366, 1770, 2914
KspAI GTTAAC 1 cut(s) 2575
KspI CCGCGG 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 1162
LmnI GCTCC 9 cut(s) 90, 180, 226, 283, 486, 549, 929, 2181, 3197
LweI GCATC 6 cut(s) 982, 1586, 1726, 1928, 2212, 2339
MaeI CTAG 5 cut(s) 798, 1380, 1926, 2310, 3243
MaeII ACGT 2 cut(s) 107, 675
MbiI CCGCTC 3 cut(s) 152, 183, 693
MfeI CAATTG 2 cut(s) 1552, 1766
MflI RGATCY 4 cut(s) 372, 1432, 2063, 3014
MhlI GDGCHC 5 cut(s) 417, 563, 1376, 1592, 3202
Mly113I GGCGCC 1 cut(s) 1065
MlyI GAGTC 8 cut(s) 547, 584, 1188, 2354, 2543, 2807, 2864, 3214
MmeI TCCRAC 3 cut(s) 590, 1999, 2242
Mph1103I ATGCAT 1 cut(s) 1921
MroI TCCGGA 1 cut(s) 483
MroXI GAANNNNTTC 3 cut(s) 2945, 3008, 3117
MseI TTAA 7 cut(s) 2015, 2297, 2444, 2574, 2732, 2747, 3000
MslI CAYNNNNRTG 2 cut(s) 1088, 1883
MspA1I CMGCKG 4 cut(s) 205, 602, 1623, 3028
MunI CAATTG 2 cut(s) 1552, 1766
MvaI CCWGG 5 cut(s) 25, 178, 1419, 2522, 2896
MvnI CGCG 8 cut(s) 52, 82, 98, 159, 174, 188, 205, 506
NarI GGCGCC 1 cut(s) 1065
NciI CCSGG 7 cut(s) 218, 350, 461, 478, 1072, 2179, 3225
NlaIII CATG 9 cut(s) 119, 430, 1087, 1529, 1574, 1687, 2558, 2584, 3052
NmuCI GTSAC 5 cut(s) 491, 523, 1885, 2103, 2867
NruI TCGCGA 1 cut(s) 506
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1921
NspI RCATGY 2 cut(s) 1087, 1687
OliI CACNNNNGTG 1 cut(s) 1883
PaqCI CACCTGC 1 cut(s) 372
PciI ACATGT 1 cut(s) 1083
PciSI GCTCTTC 1 cut(s) 1162
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 450
PdmI GAANNNNTTC 3 cut(s) 2945, 3008, 3117
PflFI GACNNNGTC 2 cut(s) 106, 394
PfoI TCCNGGA 1 cut(s) 2177
Ple19I CGATCG 1 cut(s) 453
PleI GAGTC 8 cut(s) 547, 584, 1187, 2354, 2543, 2806, 2864, 3214
PluTI GGCGCC 1 cut(s) 1068
PpsI GAGTC 8 cut(s) 547, 584, 1187, 2354, 2543, 2806, 2864, 3214
PpuMI RGGWCCY 2 cut(s) 1132, 1206
PscI ACATGT 1 cut(s) 1083
PshAI GACNNNNGTC 1 cut(s) 1554
Psp124BI GAGCTC 1 cut(s) 417
Psp5II RGGWCCY 2 cut(s) 1132, 1206
Psp6I CCWGG 5 cut(s) 23, 176, 1417, 2520, 2894
PspEI GGTNACC 1 cut(s) 1885
PspFI CCCAGC 4 cut(s) 598, 1562, 2075, 2435
PspGI CCWGG 5 cut(s) 23, 176, 1417, 2520, 2894
PspPI GGNCC 8 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249, 2503
PspPPI RGGWCCY 2 cut(s) 1132, 1206
PstI CTGCAG 2 cut(s) 213, 662
PsuI RGATCY 4 cut(s) 372, 1432, 2063, 3014
PsyI GACNNNGTC 2 cut(s) 106, 394
PvuI CGATCG 1 cut(s) 453
PvuII CAGCTG 2 cut(s) 602, 3028
RruI TCGCGA 1 cut(s) 506
RsaI GTAC 4 cut(s) 191, 197, 1313, 2670
RsaNI GTAC 4 cut(s) 190, 196, 1312, 2669
RseI CAYNNNNRTG 2 cut(s) 1088, 1883
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1162
SaqAI TTAA 7 cut(s) 2015, 2297, 2444, 2574, 2732, 2747, 3000
Sau96I GGNCC 8 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249, 2503
ScaI AGTACT 1 cut(s) 2670
SchI GAGTC 8 cut(s) 547, 584, 1188, 2354, 2543, 2807, 2864, 3214
SduI GDGCHC 5 cut(s) 417, 563, 1376, 1592, 3202
SfaNI GCATC 6 cut(s) 982, 1586, 1726, 1928, 2212, 2339
SfcI CTRYAG 5 cut(s) 209, 658, 1602, 2280, 2421
SfoI GGCGCC 1 cut(s) 1066
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 2 cut(s) 289, 520
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 7 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249
SmiMI CAYNNNNRTG 2 cut(s) 1088, 1883
SmlI CTYRAG 2 cut(s) 404, 416
SmoI CTYRAG 2 cut(s) 404, 416
SpeI ACTAGT 1 cut(s) 1925
SspDI GGCGCC 1 cut(s) 1064
SspMI CTAG 5 cut(s) 798, 1380, 1926, 2310, 3243
SstI GAGCTC 1 cut(s) 417
TaaI ACNGT 4 cut(s) 1360, 1477, 2852, 2867
TaiI ACGT 2 cut(s) 110, 678
TaqI TCGA 8 cut(s) 269, 296, 411, 441, 639, 1173, 2007, 2955
TaqII GACCGA 1 cut(s) 223
TatI WGTACW 1 cut(s) 2668
TauI GCSGC 9 cut(s) 50, 186, 205, 208, 289, 314, 346, 532, 1070
Tru1I TTAA 7 cut(s) 2015, 2297, 2444, 2574, 2732, 2747, 3000
Tru9I TTAA 7 cut(s) 2015, 2297, 2444, 2574, 2732, 2747, 3000
TseFI GTSAC 5 cut(s) 491, 523, 1885, 2103, 2867
Tsp45I GTSAC 5 cut(s) 491, 523, 1885, 2103, 2867
TspGWI ACGGA 4 cut(s) 53, 602, 693, 939
Tth111I GACNNNGTC 2 cut(s) 106, 394
VpaK11BI GGWCC 7 cut(s) 250, 595, 1132, 1206, 1238, 1934, 2249
XapI RAATTY 5 cut(s) 1518, 2003, 2651, 2734, 2996
XceI RCATGY 2 cut(s) 1087, 1687
XmiI GTMKAC 1 cut(s) 1182
XmnI GAANNNNTTC 3 cut(s) 2945, 3008, 3117
XspI CTAG 5 cut(s) 798, 1380, 1926, 2310, 3243
ZraI GACGTC 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 2670
Zsp2I ATGCAT 1 cut(s) 1921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.