RchiOBHm_Chr5g0069891
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010747
Physical Location & Seq
Forward (+)
75736385 .. 75742135
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34526

Sequence Viewer

Length: 3330 bp
ATGCCGACGCCGGTGAGCGCGGCCAGGCAATGTTTGACGGAGGAGGCGGCGCGTGCGCTGGACGACGCAGTGGCCGTGGCGCGTAGGAGGACCCACGCGCAGACGACGTCGCTTCATGCGGTGTCGGCTCTGCTCTCCCTGCCGTCGTCTGCCCTCCGCGACGCCTGCGCACGCGCCAGGAGCGGCGCGTACCCTTACACGCCGCGGCTGCAGTTCCGGGCGCTGGAGCTGTCGGTCGGGGTGTCGCTGGACCGGCTGCCGTCGTCGTCGAAGGCGCAGGAGGAGCCGCCGGTGTCGAACTCGCTGATGGCGGCGATCAAGCGGTCGCAGGCGAACCAGAGGCGGCACCCGGAGAGCTTCCACCTGCACCAGATCCACAGCCAGCAGCAAGCGGCGTCGCTTCTGAAGGTCGAGCTCAAGCACTTCATGCTGTCCATTCTTGACGACCCGATCGTGAGTCGGGTCTTCGGGGAGGCCGGGTTCCGGAGCTGTGACATAAAGCTGGCGATTGTTCACCCGCCGGTGACGCCGCAATCCCATCGGCTCCCTCGGACTCTGTGCCCGCCCATTTTCCTATGCAACCTAACGGACTCGGACCCAGCTGCCCGTCGGAGATTCCCTTTCCCGTTCTCGGGAATCGAGGAAAAGGACGAGAACTGCAGGAGAATCGGGGACGTGTTAGTTAGAAAGAGCGGGAAAAATCCGTTACTGATTGGCGTTTCCGCCACTGAAGCTCTCAAAAGCTTCATTGAAGCTGTGCAGAAGGGCAGAGCCTCCCTTTTGCCTCCTGAGGTAACTAGCTTCAGTGTAGTTAGTATGGAAGAGGAGATTTCGGAGTTTGTGGTTGAGGGTGGGAGTAGTGAAGGGAAGATGGGGTTGAAGTTGAAGGAGGTGGGTCATTTGGCTCACCAATGTTCTGGAGACGGAGGAGGAGCTGGTGTGATAGTCAACTTTGGGGAAATGAAGGCTTTGGTTGATGACGGGGTGTTGAGTGATGCTTTGAGCTTTGTGGTGGTTCAGTTGAAGGGGTTGCTGGAGATTCATAGTGGCAAGTTGTGGTTGATCGGCGCGGCCGGGAGTTACGACATGTATATGAAACTTCTGGCTCGGTTTCCGGCTATTGAGAAGGATTGGGACCTTCACCTCCTTCCCATTAGCTCTTCTAAAGCTTCAGTCGATGGAGTCTACTCTAAATCCAGCTTGATGGGGTCCTTTGTTCCATTTGGGGGATTCTTCTCTGGACCATCTGACTTTACAAATCCACTGAGCAGCACGAATCAATCTTTTATACGCTGCCATCAATGCACAGAGAAGTACGAGCAAGAAGTTGCTTCTATTCGGAAAGATGGATCAGCCATTACAGTTGGTGATCAGTGCTCAACTAGCTCACCTTCTTGGTTGCAAACGACTGAACTTGACCCTGGCAAGGGAGTGGATCTTGCAAAGACCAAAGCAGATAATACGACATTGAGTGAAACAGTATTGGGACTGCAAAAGAAATGGAATGATATCTGCCAAAAAATTCATCATGGTCAACCATTCCCTAAAATGGACAATTGTCAAGCTGGGTCTCATGTTGCATCTCCTGAGGGCTCTCATACTGCTACAGATAGAAGAGAAAGCAGCGGTGAAGATTCATCTATACAGGAAAGCCGAAGTGCAAAACATCACTGCCTTCCAATGGACACGCAAAAGAGTTTTCTGTCAAAACAGAACTTAGTAATGCAAGTAGCTTCTGATGCTGAGTATGCTGGTATGCAATCCAAGCAATTGATCAGAGATTCAAAGGGTCAGCAACTTGAACTGGGAAGCCCCTGCCGTTCACCTTTCCCTATCCCCACTATGAATCTTCCCACTGATCGCACATCATCCTCATCTATCACCTCGGTGACCACAGATTTAGGATTGGGAACCTTATATGCATCAACTAGTCAGGGACTAAGAAGTCCCAAGTTGCAAGATCATAGAGAGCGTCTCCGACACCTTTCTGGTTCCATTTCTGCTGAATTCGATACATTAAGTGAGAATAGTCCGCACCAAATAGCCCAGTCCTCTTCCTGCTCTGGATCTAATTTTGCTGGGCAGTTTGATCCAAGAGATTTCAAGTCACTTAGGAGAGTTCTCACAGCAAAGGTTTGCTGGCAAGATGAAGCAATATGCACCATTAGTGAAGCTATATCCCGGAGCAGGTCTGGTGGTGGAAGGCATCGTCGTTCAAAAGGCAGAGGAGATATATGGTTGACTTTGATTGGACCTGACAGGGTTGGAAAGAAGAAAATTGCTATAGCACTTGCAGAGTTAATGTTTGGCACTAGGGAAAGCCTAATCTCTGTTGATATGGGGGAGAGGGGATGCCAATCTGACTCGATTTTTCAATGGGAATCCCAAGATGATTATGATGTGAAGTTTAGGGGTAAGACGGCTGTAGATTATGTTGCTGGGGAATTAAGCAGGAGACCCCATTCAGTTGTCTACCTTGAAAATGTAGATAAGGCTGATTTTCTGGCCCAGAGTAACTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGATTCTCATGGCAGAGAAATCAGCATTAACAACATGATCTTTGTAACGACTTCAGCTACCAAAAAGGGCAGCAAAAATCACTACTTGGAGAATGAACCCCTGAAATTCTCAGAGGAGATGGTACTTGGTGCCAAGAGGTGTCAAATGCAAATACTGAATATTGGGGATGCTAGCCAAACGAAGGGCGTGAATGTGAGGATTGCACCAAGAGAAGGAACCTTAAATTCATCTTCTGTAAATAAGAGAAAGCTGATTGACAGCAGTGCCTCCATAGAGGAAACCTCAGAGTTACAGAAACGGGGCAACAAGGCATCAAGGTCCTTTCTGGATTTGAATCTGCCTTTTGAAGAGATAGACGAGGGAATGAACTGTGGAGATTATGACAGTGACTCCATCTCCGAAAACTCAGAAGCCTGGATGGAAGACTTCCTTGATCAAGTTGATGAAGCAGTGGTTTTGAAGCCATTCAAGTTCGACGCTCTTGCTGAGAAATTAGTGAAGGAAATCAACCAAGAATTTAAGAAGGTCTTAGGATCTGAGTATCAGCTGGAGATTGACTTTGGAGTCATGGTTCAATTACTTGCAGCTTGTTGGTTATCAGACAAGAAGAAAGCAGTGGATGAGTGGATTGAACAGGTTCTCTCCAGAAGTTTTGCTGAAGCACGTCAGAGGTATCGTCTCACTGCTCATTCTGTAATAAAATTGGTTGCCGGTGGAGCACTTTCGGTGCAAGAGCAGACACCCGGTGTTTGCCTTCCTGCTAGAATTAGTCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1109

Amino Acids

121.6

Weight (kDa)

6.75

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 116 1.2e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 216 - 390 2.3e-14 SMAX1 nucleotide binding domain
AAA_2 PF07724 748 - 877 1.2e-13 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1008 - 1087 1.5e-30 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 372
AasI GACNNNNNNGTC 2 cut(s) 1944, 3113
AatII GACGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 2 cut(s) 372, 2178
AccB1I GGYRCC 2 cut(s) 345, 2678
AccBSI CCGCTC 2 cut(s) 183, 693
AccI GTMKAC 2 cut(s) 1185, 2472
AccII CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
AccIII TCCGGA 1 cut(s) 483
AclWI GGATC 6 cut(s) 367, 1357, 1443, 2074, 2084, 3091
AcoI YGGCCR 3 cut(s) 21, 72, 1071
AcsI RAATTY 5 cut(s) 1521, 2006, 2654, 2773, 3065
AcuI CTGAAG 6 cut(s) 425, 750, 787, 1155, 2586, 3228
AcyI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
AdeI CACNNNGTG 1 cut(s) 3296
AfaI GTAC 3 cut(s) 191, 1316, 2673
AflIII ACRYGT 2 cut(s) 675, 1086
AhlI ACTAGT 1 cut(s) 1928
AjiI CACGTC 2 cut(s) 676, 3215
AjnI CCWGG 5 cut(s) 23, 176, 1420, 2523, 2963
AleI CACNNNNGTG 1 cut(s) 1886
AloI GAACNNNNNNTCC 4 cut(s) 464, 496, 3105, 3137
Alw21I GWGCWC 3 cut(s) 417, 1379, 3271
Alw26I GTCTC 5 cut(s) 915, 1575, 1979, 2449, 3233
AlwI GGATC 6 cut(s) 367, 1357, 1443, 2074, 2084, 3091
Ama87I CYCGRG 1 cut(s) 631
Aor13HI TCCGGA 1 cut(s) 483
AoxI GGCC 6 cut(s) 21, 72, 474, 1071, 2505, 2526
ApeKI GCWGC 9 cut(s) 208, 256, 385, 602, 1269, 1293, 1623, 2619, 3134
ApoI RAATTY 5 cut(s) 1521, 2006, 2654, 2773, 3065
Asp700I GAANNNNTTC 2 cut(s) 3014, 3186
AspS9I GGNCC 9 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2506, 2868
AsuC2I CCSGG 6 cut(s) 218, 350, 478, 1075, 2182, 3294
AsuNHI GCTAGC 1 cut(s) 2720
AvaI CYCGRG 1 cut(s) 631
AvaII GGWCC 8 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2868
AxyI CCTNAGG 2 cut(s) 789, 1587
BaeGI GKGCMC 1 cut(s) 563
BanI GGYRCC 2 cut(s) 345, 2678
BanII GRGCYC 2 cut(s) 417, 1595
BbsI GAAGAC 2 cut(s) 457, 2979
Bbv12I GWGCWC 3 cut(s) 417, 1379, 3271
BbvI GCAGC 9 cut(s) 195, 243, 397, 589, 1280, 1281, 1635, 2631, 3146
BceAI ACGGC 5 cut(s) 59, 127, 244, 1803, 2436
BcgI CGANNNNNNTGC 6 cut(s) 521, 555, 649, 683, 3014, 3048
BciT130I CCWGG 5 cut(s) 25, 178, 1422, 2525, 2965
BclI TGATCA 3 cut(s) 1369, 1773, 2983
BcnI CCSGG 6 cut(s) 218, 350, 478, 1075, 2182, 3294
BcoDI GTCTC 5 cut(s) 915, 1575, 1979, 2449, 3233
BcuI ACTAGT 1 cut(s) 1928
BfaI CTAG 6 cut(s) 798, 1383, 1929, 2313, 2721, 3312
BfmI CTRYAG 5 cut(s) 209, 658, 1605, 2283, 2424
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 2 cut(s) 372, 2178
Bme18I GGWCC 8 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2868
BmeT110I CYCGRG 1 cut(s) 631
BmgBI CACGTC 2 cut(s) 676, 3215
BmgT120I GGNCC 9 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2506, 2868
BmrI ACTGGG 2 cut(s) 1814, 2041
BmsI GCATC 8 cut(s) 985, 1589, 1729, 1931, 2215, 2342, 2707, 2870
BmtI GCTAGC 1 cut(s) 2724
BmuI ACTGGG 2 cut(s) 1814, 2041
BoxI GACNNNNGTC 1 cut(s) 1557
BpiI GAAGAC 2 cut(s) 457, 2979
BplI GAGNNNNNCTC 4 cut(s) 1959, 1991, 2816, 2848
BpmI CTGGAG 5 cut(s) 245, 939, 1055, 3119, 3178
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 6 cut(s) 218, 350, 478, 1075, 2182, 3294
BsaBI GATNNNNATC 2 cut(s) 2356, 2883
BsaHI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BsaI GGTCTC 2 cut(s) 1575, 2449
BsaJI CCNNGG 6 cut(s) 75, 203, 548, 1420, 1884, 2523
BsaWI WCCGGW 1 cut(s) 483
Bse118I RCCGGY 5 cut(s) 10, 252, 289, 520, 3260
Bse1I ACTGG 3 cut(s) 1809, 2047, 2542
Bse21I CCTNAGG 2 cut(s) 789, 1587
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 2 cut(s) 2356, 2883
BseAI TCCGGA 1 cut(s) 483
BseBI CCWGG 5 cut(s) 25, 178, 1422, 2525, 2965
BseDI CCNNGG 6 cut(s) 75, 203, 548, 1420, 1884, 2523
BseGI GGATG 5 cut(s) 1868, 2357, 2722, 2973, 3175
BseJI GATNNNNATC 2 cut(s) 2356, 2883
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 9 cut(s) 780, 1256, 1578, 1734, 2673, 2847, 2970, 3027, 3078
BseNI ACTGG 3 cut(s) 1809, 2047, 2542
BseRI GAGGAG 7 cut(s) 56, 296, 839, 942, 945, 2241, 2678
BseSI GKGCMC 1 cut(s) 563
BseX3I CGGCCG 1 cut(s) 1071
BseXI GCAGC 9 cut(s) 195, 243, 397, 589, 1280, 1281, 1635, 2631, 3146
BseYI CCCAGC 4 cut(s) 598, 1565, 2078, 2438
BsgI GTGCAG 2 cut(s) 350, 779
Bsh1236I CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
Bsh1285I CGRYCG 4 cut(s) 237, 326, 453, 1074
BshFI GGCC 6 cut(s) 23, 74, 476, 1073, 2507, 2528
BshNI GGYRCC 2 cut(s) 345, 2678
BsiEI CGRYCG 4 cut(s) 237, 326, 453, 1074
BsiHKAI GWGCWC 3 cut(s) 417, 1379, 3271
BsiHKCI CYCGRG 1 cut(s) 631
BslFI GGGAC 6 cut(s) 686, 1148, 1500, 1932, 1950, 2506
BsmAI GTCTC 5 cut(s) 915, 1575, 1979, 2449, 3233
BsmBI CGTCTC 3 cut(s) 915, 1979, 3233
BsmFI GGGAC 6 cut(s) 686, 1148, 1500, 1932, 1950, 2506
BsnI GGCC 6 cut(s) 23, 74, 476, 1073, 2507, 2528
Bso31I GGTCTC 2 cut(s) 1575, 2449
BsoBI CYCGRG 1 cut(s) 631
Bsp1286I GDGCHC 5 cut(s) 417, 563, 1379, 1595, 3271
Bsp13I TCCGGA 1 cut(s) 483
BspANI GGCC 6 cut(s) 23, 74, 476, 1073, 2507, 2528
BspCNI CTCAG 9 cut(s) 781, 1257, 1579, 1735, 2672, 2846, 2969, 3028, 3079
BspEI TCCGGA 1 cut(s) 483
BspFNI CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
BspMAI CTGCAG 2 cut(s) 213, 662
BspMI ACCTGC 2 cut(s) 372, 2178
BspOI GCTAGC 1 cut(s) 2724
BspPI GGATC 6 cut(s) 367, 1357, 1443, 2074, 2084, 3091
BspQI GCTCTTC 1 cut(s) 1165
BspT107I GGYRCC 2 cut(s) 345, 2678
BspTNI GGTCTC 2 cut(s) 1575, 2449
BsrBI CCGCTC 2 cut(s) 183, 693
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 5 cut(s) 10, 252, 289, 520, 3260
BsrI ACTGG 3 cut(s) 1809, 2047, 2542
BssAI RCCGGY 5 cut(s) 10, 252, 289, 520, 3260
BssECI CCNNGG 6 cut(s) 75, 203, 548, 1420, 1884, 2523
BssNI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Bst2UI CCWGG 5 cut(s) 25, 178, 1422, 2525, 2965
Bst4CI ACNGT 4 cut(s) 1363, 1480, 2921, 2936
Bst6I CTCTTC 5 cut(s) 816, 1165, 1609, 2059, 2892
BstACI GRCGYC 5 cut(s) 8, 107, 162, 395, 527
BstAPI GCANNNNNTGC 1 cut(s) 427
BstDSI CCRYGG 2 cut(s) 75, 203
BstEII GGTNACC 1 cut(s) 1888
BstF5I GGATG 5 cut(s) 1868, 2357, 2722, 2973, 3175
BstFNI CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
BstH2I RGCGCY 1 cut(s) 224
BstMAI GTCTC 5 cut(s) 915, 1575, 1979, 2449, 3233
BstMCI CGRYCG 4 cut(s) 237, 326, 453, 1074
BstNI CCWGG 5 cut(s) 25, 178, 1422, 2525, 2965
BstNSI RCATGY 1 cut(s) 1090
BstPAI GACNNNNGTC 1 cut(s) 1557
BstPI GGTNACC 1 cut(s) 1888
BstSFI CTRYAG 5 cut(s) 209, 658, 1605, 2283, 2424
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
BstV1I GCAGC 9 cut(s) 195, 243, 397, 589, 1280, 1281, 1635, 2631, 3146
BstV2I GAAGAC 2 cut(s) 457, 2979
BstX2I RGATCY 4 cut(s) 372, 1435, 2066, 3083
BstXI CCANNNNNNTGG 2 cut(s) 917, 1204
BstYI RGATCY 4 cut(s) 372, 1435, 2066, 3083
BstZI CGGCCG 1 cut(s) 1071
Bsu36I CCTNAGG 2 cut(s) 789, 1587
BsuRI GGCC 6 cut(s) 23, 74, 476, 1073, 2507, 2528
BtgI CCRYGG 2 cut(s) 75, 203
BtrI CACGTC 2 cut(s) 676, 3215
BtsCI GGATG 5 cut(s) 1868, 2357, 2722, 2973, 3175
BtsI GCAGTG 6 cut(s) 75, 1669, 2818, 3006, 3171, 3231
BveI ACCTGC 2 cut(s) 372, 2178
Cfr10I RCCGGY 5 cut(s) 10, 252, 289, 520, 3260
Cfr13I GGNCC 9 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2506, 2868
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1961, 3035
Csp6I GTAC 3 cut(s) 190, 1315, 2672
CviAII CATG 8 cut(s) 116, 427, 1087, 1529, 1574, 2558, 2584, 3118
CviQI GTAC 3 cut(s) 190, 1315, 2672
DraIII CACNNNGTG 1 cut(s) 3296
DrdI GACNNNNNNGTC 2 cut(s) 1944, 3113
DseDI GACNNNNNNGTC 2 cut(s) 1944, 3113
EaeI YGGCCR 3 cut(s) 21, 72, 1071
EagI CGGCCG 1 cut(s) 1071
Eam1104I CTCTTC 5 cut(s) 816, 1165, 1609, 2059, 2892
EarI CTCTTC 5 cut(s) 816, 1165, 1609, 2059, 2892
EciI GGCGGA 1 cut(s) 712
Ecl136II GAGCTC 1 cut(s) 415
EclXI CGGCCG 1 cut(s) 1071
Eco24I GRGCYC 2 cut(s) 417, 1595
Eco31I GGTCTC 2 cut(s) 1575, 2449
Eco32I GATATC 1 cut(s) 1510
Eco47I GGWCC 8 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2868
Eco52I CGGCCG 1 cut(s) 1071
Eco53kI GAGCTC 1 cut(s) 415
Eco57I CTGAAG 6 cut(s) 425, 750, 787, 1155, 2586, 3228
Eco81I CCTNAGG 2 cut(s) 789, 1587
Eco88I CYCGRG 1 cut(s) 631
Eco91I GGTNACC 1 cut(s) 1888
EcoICRI GAGCTC 1 cut(s) 415
EcoO109I RGGNCCY 4 cut(s) 90, 1135, 1209, 2868
EcoO65I GGTNACC 1 cut(s) 1888
EcoRI GAATTC 1 cut(s) 2006
EcoRII CCWGG 5 cut(s) 23, 176, 1420, 2523, 2963
EcoRV GATATC 1 cut(s) 1510
EcoT22I ATGCAT 1 cut(s) 1924
EcoT38I GRGCYC 2 cut(s) 417, 1595
Esp3I CGTCTC 3 cut(s) 915, 1979, 3233
FaeI CATG 8 cut(s) 119, 430, 1090, 1532, 1577, 2561, 2587, 3121
FalI AAGNNNNNCTT 4 cut(s) 2964, 2996, 3062, 3094
FaqI GGGAC 6 cut(s) 686, 1148, 1500, 1932, 1950, 2506
FatI CATG 8 cut(s) 115, 426, 1086, 1528, 1573, 2557, 2583, 3117
FauI CCCGC 3 cut(s) 525, 570, 686
FbaI TGATCA 3 cut(s) 1369, 1773, 2983
FblI GTMKAC 2 cut(s) 1185, 2472
FokI GGATG 5 cut(s) 1855, 2364, 2729, 2980, 3182
FriOI GRGCYC 2 cut(s) 417, 1595
FspBI CTAG 6 cut(s) 798, 1383, 1929, 2313, 2721, 3312
FspI TGCGCA 1 cut(s) 169
GsaI CCCAGC 4 cut(s) 602, 1569, 2082, 2442
GsuI CTGGAG 5 cut(s) 245, 939, 1055, 3119, 3178
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 6 cut(s) 23, 74, 476, 1073, 2507, 2528
HgaI GACGC 7 cut(s) 16, 74, 170, 384, 535, 1961, 3035
Hin1I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hin1II CATG 8 cut(s) 119, 430, 1090, 1532, 1577, 2561, 2587, 3121
HincII GTYRAC 3 cut(s) 949, 1535, 2241
HindII GTYRAC 3 cut(s) 949, 1535, 2241
HindIII AAGCTT 2 cut(s) 742, 1167
Hpy166II GTNNAC 7 cut(s) 514, 949, 1186, 1535, 1823, 2241, 2473
Hpy8I GTNNAC 7 cut(s) 514, 949, 1186, 1535, 1823, 2241, 2473
HpyCH4III ACNGT 4 cut(s) 1363, 1480, 2921, 2936
HpyCH4IV ACGT 3 cut(s) 107, 675, 3214
HpySE526I ACGT 3 cut(s) 107, 675, 3214
Hsp92I GRCGYC 5 cut(s) 8, 107, 162, 395, 527
Hsp92II CATG 8 cut(s) 119, 430, 1090, 1532, 1577, 2561, 2587, 3121
Kpn2I TCCGGA 1 cut(s) 483
Ksp22I TGATCA 3 cut(s) 1369, 1773, 2983
KspI CCGCGG 1 cut(s) 206
LguI GCTCTTC 1 cut(s) 1165
LmnI GCTCC 8 cut(s) 180, 226, 283, 486, 549, 932, 2184, 3266
Lsp1109I GCAGC 9 cut(s) 195, 243, 397, 589, 1280, 1281, 1635, 2631, 3146
LweI GCATC 8 cut(s) 985, 1589, 1729, 1931, 2215, 2342, 2707, 2870
MaeI CTAG 6 cut(s) 798, 1383, 1929, 2313, 2721, 3312
MaeII ACGT 3 cut(s) 107, 675, 3214
MbiI CCGCTC 2 cut(s) 183, 693
MfeI CAATTG 2 cut(s) 1555, 1769
MflI RGATCY 4 cut(s) 372, 1435, 2066, 3083
MhlI GDGCHC 5 cut(s) 417, 563, 1379, 1595, 3271
MlyI GAGTC 7 cut(s) 466, 547, 584, 1191, 2357, 2933, 3123
MmeI TCCRAC 3 cut(s) 590, 2002, 2245
Mph1103I ATGCAT 1 cut(s) 1924
MroI TCCGGA 1 cut(s) 483
MroXI GAANNNNTTC 2 cut(s) 3014, 3186
MseI TTAA 6 cut(s) 2018, 2300, 2447, 2577, 2771, 3069
MslI CAYNNNNRTG 2 cut(s) 1091, 1886
MspA1I CMGCKG 4 cut(s) 205, 602, 1626, 3097
MunI CAATTG 2 cut(s) 1555, 1769
MvaI CCWGG 5 cut(s) 25, 178, 1422, 2525, 2965
MvnI CGCG 9 cut(s) 20, 52, 82, 98, 159, 174, 188, 205, 1070
NciI CCSGG 6 cut(s) 218, 350, 478, 1075, 2182, 3294
NheI GCTAGC 1 cut(s) 2720
NlaIII CATG 8 cut(s) 119, 430, 1090, 1532, 1577, 2561, 2587, 3121
NmuCI GTSAC 5 cut(s) 491, 523, 1888, 2106, 2936
NsbI TGCGCA 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1924
NspI RCATGY 1 cut(s) 1090
OliI CACNNNNGTG 1 cut(s) 1886
PaqCI CACCTGC 1 cut(s) 372
PciI ACATGT 1 cut(s) 1086
PciSI GCTCTTC 1 cut(s) 1165
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 450
PdmI GAANNNNTTC 2 cut(s) 3014, 3186
PflFI GACNNNGTC 1 cut(s) 106
PfoI TCCNGGA 1 cut(s) 2180
Ple19I CGATCG 1 cut(s) 453
PleI GAGTC 7 cut(s) 465, 547, 584, 1190, 2357, 2933, 3122
PpsI GAGTC 7 cut(s) 465, 547, 584, 1190, 2357, 2933, 3122
PpuMI RGGWCCY 4 cut(s) 90, 1135, 1209, 2868
PscI ACATGT 1 cut(s) 1086
PshAI GACNNNNGTC 1 cut(s) 1557
Psp124BI GAGCTC 1 cut(s) 417
Psp5II RGGWCCY 4 cut(s) 90, 1135, 1209, 2868
Psp6I CCWGG 5 cut(s) 23, 176, 1420, 2523, 2963
PspEI GGTNACC 1 cut(s) 1888
PspFI CCCAGC 4 cut(s) 598, 1565, 2078, 2438
PspGI CCWGG 5 cut(s) 23, 176, 1420, 2523, 2963
PspPI GGNCC 9 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2506, 2868
PspPPI RGGWCCY 4 cut(s) 90, 1135, 1209, 2868
PstI CTGCAG 2 cut(s) 213, 662
PsuI RGATCY 4 cut(s) 372, 1435, 2066, 3083
PsyI GACNNNGTC 1 cut(s) 106
PvuI CGATCG 1 cut(s) 453
PvuII CAGCTG 2 cut(s) 602, 3097
RsaI GTAC 3 cut(s) 191, 1316, 2673
RsaNI GTAC 3 cut(s) 190, 1315, 2672
RseI CAYNNNNRTG 2 cut(s) 1091, 1886
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 206
SapI GCTCTTC 1 cut(s) 1165
SaqAI TTAA 6 cut(s) 2018, 2300, 2447, 2577, 2771, 3069
Sau96I GGNCC 9 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2506, 2868
SchI GAGTC 7 cut(s) 466, 547, 584, 1191, 2357, 2933, 3123
SduI GDGCHC 5 cut(s) 417, 563, 1379, 1595, 3271
SfaNI GCATC 8 cut(s) 985, 1589, 1729, 1931, 2215, 2342, 2707, 2870
SfcI CTRYAG 5 cut(s) 209, 658, 1605, 2283, 2424
Sfr303I CCGCGG 1 cut(s) 206
SgrAI CRCCGGYG 3 cut(s) 10, 289, 520
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 8 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2868
SmiMI CAYNNNNRTG 2 cut(s) 1091, 1886
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 1928
SspI AATATT 1 cut(s) 2710
SspMI CTAG 6 cut(s) 798, 1383, 1929, 2313, 2721, 3312
SstI GAGCTC 1 cut(s) 417
TaaI ACNGT 4 cut(s) 1363, 1480, 2921, 2936
TaiI ACGT 3 cut(s) 110, 678, 3217
TaqI TCGA 8 cut(s) 269, 296, 411, 639, 1176, 2010, 2366, 3024
TaqII GACCGA 1 cut(s) 223
Tru1I TTAA 6 cut(s) 2018, 2300, 2447, 2577, 2771, 3069
Tru9I TTAA 6 cut(s) 2018, 2300, 2447, 2577, 2771, 3069
TseFI GTSAC 5 cut(s) 491, 523, 1888, 2106, 2936
TseI GCWGC 9 cut(s) 208, 256, 385, 602, 1269, 1293, 1623, 2619, 3134
Tsp45I GTSAC 5 cut(s) 491, 523, 1888, 2106, 2936
TspGWI ACGGA 4 cut(s) 53, 602, 693, 939
Tth111I GACNNNGTC 1 cut(s) 106
VpaK11BI GGWCC 8 cut(s) 90, 250, 595, 1135, 1209, 1241, 2252, 2868
XapI RAATTY 5 cut(s) 1521, 2006, 2654, 2773, 3065
XceI RCATGY 1 cut(s) 1090
XmiI GTMKAC 2 cut(s) 1185, 2472
XmnI GAANNNNTTC 2 cut(s) 3014, 3186
XspI CTAG 6 cut(s) 798, 1383, 1929, 2313, 2721, 3312
ZraI GACGTC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 1924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.