Rh5DG488300
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010747
Physical Location & Seq
Forward (+)
76716837 .. 76723319
6483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG488300.1

Sequence Viewer

Length: 3315 bp
ATGCCGACGCCGGTAGGCGCAGCCAGGCAATGTTTGACGGAGGAGGCGGCGCGTGCGCTGGACGACGCAGTGGCCGTGGCGCGTAGGAGGAGCCACGCGCAGACGACGTCGCTCCATGCGGTGTCGGCTCTGCTCTCCCTGCCGTCCTCTGCTCTCCGCGACGCCTGCTCACGCGCCAGGAGCGGCGTGTACCCGCCGTACACGCCGCGGCTCCAGTTCCGGGCGCTGGAGCTGTCCGTCGGGGTGTCGCTGGACCGGCTGCCGTCGTCGACGAAGGCGCAGGAGGAGCCGCCGGTGTCGAACTCGCTGATGGCGGCGATCAAGCGGTCGCAGGCGAACCAGAGGCGGCACCCGGAGAGCTTCCACCTGCACCAGATCCACAGCCAGCAGCAGACGGCGTCGCTTCTGAAGGTCGAGCTCAAGCACTTCATGCTGTCCATTCTCGACGACCCGATTGTGAGTCGGGTCTTCGGGGAGGCCGGGTTCCGGAGCTGTGACATAAAGCTCGCGATTGTTCACCCGCCGGTGACGCCGCAATCCAATCGGCTGCCTCGGACTCTGTGCCCGCCCATTTTCCTATGCAACCTAACGGACTCGGACCCCGCTGCCCGTCGGAGATTCCCTTTCCCGTTCTCGGGGTTCGAGGAAAAGGACGAGAACTGCAAGAGAATCGGGGACGTGTTGGTTAGAAAGAGCGGGAAAAATCCGTTACTGATCGGCGTTTCCGCCGCTGAAGCTCTCAAAAGGTTCATTGAAGCGGTACAGAAAGGCAGAGCCTCCTCCCTTTTGCCTCCTGAGGTAAGTAGCTTTAGTGTAGTTAGTATGGAAGAGGAGATTTCGGAGTTTGTGGTTGAGGGTGGGAGTGAAGAGAAGATGGGGTTGAAGTTGAAGGAGGTGGGTCATTTGGCTCAGCAATGTTCTGGTGTGATAGTCAACTTTGGGGAAATGAAGGCTTTGGTTGATGACGGGGTGTTGAGTGATGCTTTGAGCTTTGTGGTGGTTCAGTTGAAGGGGTTGCTGGAGATTCATAGTGGCAAGTTGTGGTTGATCGGCGCGGCGGGGAGTTACGACATGTATATGAAACTTTTGGCTCGGTTTCCGGCTATCGAGAAGGATTGGGACCTTCATCTCCTTCCCATTAGCTCTTCTAAAGCTTCAGTCGATGGAGTCTACTCTAAATCCAGCTTGATGGGGTCCTTTGTTCCATTTGGGGGATTCTTCTCTGGACCATCTGACTTTACAAATCCACCGAGCAGCACGAATCAATCTTTTATACGCTGCCATCAATGCACAGAGAAGTATGAGCAAGAAGTTGCTTCTATTCGGAAAGATGGATCAGCCATTACAGTTGGTGATCAGTGCTCAACTAGCTCACCTTCTTGGTTGCAAACGATTGAACTTGACCCTGGCAAGGGAGTGGATCTTGCAAAGACCAAAGCAGATAATACGACATTGAGTGAAACAGTATTGGGACTGCAAAAGAAATGGAATGATATCTGCCGAAAAATTCATCATGGTCAACCATTCCCTAAAATGGACAATTGTCAAGCTGGGTCTCATGTTGCATCTCCTGAGGGCTCTCATACTGCTGCAGATAGAAGAGAAAGCAGCGGTGAAGATTCATCTATACAGGAAAGCCGAAGTGCAAAACATCACTGCCTTCCAATGGACATGCAAAAGAGTTTTCTGTCAAAACAGAACTTGGTAATGCAAGTAGCTTCTGATGCTGAGTATGCTGGTATGCAATCCAAGCAATTGATCAGAGATTCAAAGGGTCAGCAACTTGAACTGGGAAGCCCCTGCCGTTCACCTTTCCCAATCCCCACTATGAATCTTCCCACTGATCGCACATCATCCTCATCTGTCACTTCGGTGACCACAGATTTAGGATTGGGAACCTTATATGCATCAACTAGAAAGGGACCAAGAAGTCCCAAGTTGCAAGATCATAGAGAGCGTCTCCGACACCTTTCTGGTTCCATTTCTGCTGAATTCGATGCATTAAGTGAGAATAGTCCGCACCAAATAGCCCAGTCCTCTTCCTGCTCTGGATCTAATTTTGGTGGGCAGTTTGATCCAAGAGATTTCAAGTCACTTAGGAGAGTTCTCACAGAAAAGGTTTACTGGCAAGATGAAGCAATATGCACCATTAGTGAAGCTATATCCCGGTGCAGGTCTGGTGGTGGAAGGCATCATGGTTCAAAAGGCAGAGGAGATATATGGTTGACTTTGATTGGACCCGACAGGGTTGGAAAGAAGAAAATTGCTATAGCACTTGCAGAGTTAATGTTTGGAACTAGGGAAAGCCTAATCTCTGTTGATATGGGGGAGAGGGGATGCCAATCTGACTCTATTTTTCAATGGGAATCCCAAGATGATTATGATGTGAAGTTTAGGGGTAAGACGGCTGTAGATTATGTTGCTGGGGAATTAAGCAGGAGACCCCATTCAGTTGTCTTCCTTGAAAATGTAGATAAGGCTGATTTTCTGGCCCAGAGTAACTTGTCCCAGGCCATTAGGACTGGCAAGTTTCCAGACTCTCACGGCAGAGAAATCAGCATTAACAACATGATCTTTGTAACGACTTCAGCTACCAAAAAGGGCAGCAAAAATCACTACTTGGAGAATGAACCCCTGAAATTCTCTGAGGAGATGGTACTTGGTGCCAAGAGGTGTCAAATGCAAATACTGAATATTGGGGATGCCAGCCAAACGAAGGGCATGAATGTGAGGATTGCACCAAGAGAAGGAAGCTTCAACTCATCTTCTGTAAATAAGAGAAAGCTGATTGACAGCAGTGCCTCCATAGAGGAAATCTCAGAGTTACAGAAACGGGGCAACAAGGCATCAAGGTCCTTTCTGGATTTGAATCTGCCTGTTGAAGAGATAGACGAGGGAATGAACTGTGGAGATTATGACAGTGACTCCATCTCCGAAAACTCAGAAGCCTGGATGGAAGACTTCCTTGATCAAGTTGATGAAGCAGTGGTTTTGAAGCCATTCAAGTTCGACGCTCTTGCTGAGAAATTAGTAAAGGAAATCAACCAAGAATTTAAGAAAGTCTTCGGATCTGAGTATCAGCTGGAGATTGACTTTGGAGTCATGGTTCAATTACTTGCAGCTTGTTGGTTATCAGACAAGAAGAAAGCAGTGGAGGAGTGGATTGAACAGGTTCTCTCCAGAAGTTTTGCTGAAACACGTCAGAGGTATCGTCTCACTGCTCATTCTGTAATAAAATTGGTTGCCGGTGGAGCACTTTCGGTGCAAGAGCAGACTCCCGGTGTTTGCCTTCCTGCTAGAATTAGTCTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

1104

Amino Acids

121.51

Weight (kDa)

6.85

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clp_N PF02861 12 - 117 1.9e-06 Clp repeat (R) N-terminal domain
NBD_SMAX1 PF23569 217 - 385 8.5e-15 SMAX1 nucleotide binding domain
AAA_2 PF07724 743 - 872 7.3e-14 AAA domain (Cdc48 subfamily)
AAA_lid_SMAX1 PF26587 1003 - 1082 7.9e-31 SMAX1-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 375
AasI GACNNNNNNGTC 2 cut(s) 1929, 3098
AatII GACGTC 1 cut(s) 110
Acc36I ACCTGC 2 cut(s) 375, 2163
AccB1I GGYRCC 2 cut(s) 348, 2663
AccBSI CCGCTC 2 cut(s) 183, 696
AccI GTMKAC 2 cut(s) 269, 1170
AccII CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
AccIII TCCGGA 1 cut(s) 486
AclWI GGATC 6 cut(s) 370, 1342, 1428, 2059, 2069, 3076
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 4 cut(s) 1506, 1991, 2639, 3050
AcuI CTGAAG 4 cut(s) 428, 753, 1140, 2571
AcyI GRCGYC 5 cut(s) 8, 107, 162, 398, 530
AfaI GTAC 4 cut(s) 191, 200, 762, 2658
AflIII ACRYGT 3 cut(s) 678, 1071, 3197
AjiI CACGTC 2 cut(s) 679, 3200
AjnI CCWGG 5 cut(s) 23, 176, 1405, 2508, 2948
AleI CACNNNNGTG 1 cut(s) 1871
AloI GAACNNNNNNTCC 4 cut(s) 467, 499, 3090, 3122
Alw21I GWGCWC 3 cut(s) 420, 1364, 3256
Alw26I GTCTC 4 cut(s) 1560, 1964, 2434, 3218
AlwI GGATC 6 cut(s) 370, 1342, 1428, 2059, 2069, 3076
Ama87I CYCGRG 1 cut(s) 634
Aor13HI TCCGGA 1 cut(s) 486
AoxI GGCC 4 cut(s) 72, 477, 2490, 2511
ApoI RAATTY 4 cut(s) 1506, 1991, 2639, 3050
Asp700I GAANNNNTTC 3 cut(s) 2999, 3062, 3171
AspLEI GCGC 9 cut(s) 20, 52, 58, 82, 100, 176, 226, 280, 1055
AspS9I GGNCC 9 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2491, 2853
AsuC2I CCSGG 5 cut(s) 221, 353, 481, 2167, 3279
AsuHPI GGTGA 7 cut(s) 509, 538, 1364, 1365, 1625, 1800, 1885
AvaI CYCGRG 1 cut(s) 634
AvaII GGWCC 8 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2853
AxyI CCTNAGG 2 cut(s) 795, 1572
BaeGI GKGCMC 1 cut(s) 566
BanI GGYRCC 2 cut(s) 348, 2663
BanII GRGCYC 2 cut(s) 420, 1580
BbsI GAAGAC 4 cut(s) 460, 2449, 2964, 3055
Bbv12I GWGCWC 3 cut(s) 420, 1364, 3256
BceAI ACGGC 8 cut(s) 59, 127, 181, 247, 411, 1788, 2421, 2560
BcgI CGANNNNNNTGC 6 cut(s) 524, 558, 652, 686, 2999, 3033
BciT130I CCWGG 5 cut(s) 25, 178, 1407, 2510, 2950
BclI TGATCA 3 cut(s) 1354, 1758, 2968
BcnI CCSGG 5 cut(s) 221, 353, 481, 2167, 3279
BcoDI GTCTC 4 cut(s) 1560, 1964, 2434, 3218
BfaI CTAG 4 cut(s) 1368, 1914, 2298, 3297
BfmI CTRYAG 3 cut(s) 1590, 2268, 2409
BfoI RGCGCY 1 cut(s) 227
BfuAI ACCTGC 2 cut(s) 375, 2163
BlpI GCTNAGC 1 cut(s) 909
Bme18I GGWCC 8 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2853
BmeT110I CYCGRG 1 cut(s) 634
BmgBI CACGTC 2 cut(s) 679, 3200
BmgT120I GGNCC 9 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2491, 2853
BmrI ACTGGG 2 cut(s) 1799, 2026
BmsI GCATC 9 cut(s) 970, 1574, 1714, 1916, 1987, 2200, 2327, 2692, 2855
BmuI ACTGGG 2 cut(s) 1799, 2026
BoxI GACNNNNGTC 1 cut(s) 1542
BpiI GAAGAC 4 cut(s) 460, 2449, 2964, 3055
BplI GAGNNNNNCTC 4 cut(s) 1944, 1976, 2801, 2833
BpmI CTGGAG 5 cut(s) 197, 248, 1040, 3104, 3163
Bpu1102I GCTNAGC 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 5 cut(s) 221, 353, 481, 2167, 3279
BsaBI GATNNNNATC 2 cut(s) 2341, 2868
BsaHI GRCGYC 5 cut(s) 8, 107, 162, 398, 530
BsaI GGTCTC 2 cut(s) 1560, 2434
BsaJI CCNNGG 5 cut(s) 75, 206, 551, 1405, 2508
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 8 cut(s) 467, 497, 2909, 2915, 2939, 2945, 3090, 3120
Bse118I RCCGGY 5 cut(s) 10, 255, 292, 523, 3245
Bse1I ACTGG 5 cut(s) 214, 1794, 2032, 2129, 2527
Bse21I CCTNAGG 2 cut(s) 795, 1572
Bse3DI GCAATG 2 cut(s) 35, 920
Bse8I GATNNNNATC 2 cut(s) 2341, 2868
BseAI TCCGGA 1 cut(s) 486
BseBI CCWGG 5 cut(s) 25, 178, 1407, 2510, 2950
BseDI CCNNGG 5 cut(s) 75, 206, 551, 1405, 2508
BseGI GGATG 4 cut(s) 1853, 2342, 2707, 2958
BseJI GATNNNNATC 2 cut(s) 2341, 2868
BseMI GCAATG 2 cut(s) 35, 920
BseMII CTCAG 9 cut(s) 786, 923, 1563, 1719, 2637, 2832, 2955, 3012, 3063
BseNI ACTGG 5 cut(s) 214, 1794, 2032, 2129, 2527
BseRI GAGGAG 8 cut(s) 56, 103, 299, 769, 845, 2226, 2663, 3170
BseSI GKGCMC 1 cut(s) 566
BseYI CCCAGC 2 cut(s) 1550, 2423
BsgI GTGCAG 2 cut(s) 353, 2191
Bsh1236I CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
Bsh1285I CGRYCG 1 cut(s) 329
BshFI GGCC 4 cut(s) 74, 479, 2492, 2513
BshNI GGYRCC 2 cut(s) 348, 2663
BsiEI CGRYCG 1 cut(s) 329
BsiHKAI GWGCWC 3 cut(s) 420, 1364, 3256
BsiHKCI CYCGRG 1 cut(s) 634
BslFI GGGAC 6 cut(s) 689, 1133, 1485, 1917, 1935, 2491
BsmAI GTCTC 4 cut(s) 1560, 1964, 2434, 3218
BsmBI CGTCTC 2 cut(s) 1964, 3218
BsmFI GGGAC 6 cut(s) 689, 1133, 1485, 1917, 1935, 2491
BsnI GGCC 4 cut(s) 74, 479, 2492, 2513
Bso31I GGTCTC 2 cut(s) 1560, 2434
BsoBI CYCGRG 1 cut(s) 634
Bsp1286I GDGCHC 5 cut(s) 420, 566, 1364, 1580, 3256
Bsp13I TCCGGA 1 cut(s) 486
Bsp1720I GCTNAGC 1 cut(s) 909
Bsp68I TCGCGA 1 cut(s) 509
BspANI GGCC 4 cut(s) 74, 479, 2492, 2513
BspCNI CTCAG 9 cut(s) 787, 922, 1564, 1720, 2638, 2831, 2954, 3013, 3064
BspEI TCCGGA 1 cut(s) 486
BspFNI CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
BspMAI CTGCAG 1 cut(s) 1594
BspMI ACCTGC 2 cut(s) 375, 2163
BspPI GGATC 6 cut(s) 370, 1342, 1428, 2059, 2069, 3076
BspQI GCTCTTC 1 cut(s) 1150
BspT107I GGYRCC 2 cut(s) 348, 2663
BspTNI GGTCTC 2 cut(s) 1560, 2434
BsrBI CCGCTC 2 cut(s) 183, 696
BsrDI GCAATG 2 cut(s) 35, 920
BsrFI RCCGGY 5 cut(s) 10, 255, 292, 523, 3245
BsrI ACTGG 5 cut(s) 214, 1794, 2032, 2129, 2527
BssAI RCCGGY 5 cut(s) 10, 255, 292, 523, 3245
BssECI CCNNGG 5 cut(s) 75, 206, 551, 1405, 2508
BssNI GRCGYC 5 cut(s) 8, 107, 162, 398, 530
Bst2UI CCWGG 5 cut(s) 25, 178, 1407, 2510, 2950
Bst4CI ACNGT 4 cut(s) 1348, 1465, 2906, 2921
Bst6I CTCTTC 6 cut(s) 822, 861, 1150, 1594, 2044, 2877
BstACI GRCGYC 5 cut(s) 8, 107, 162, 398, 530
BstAPI GCANNNNNTGC 1 cut(s) 430
BstC8I GCNNGC 7 cut(s) 54, 166, 333, 386, 507, 566, 2707
BstDSI CCRYGG 2 cut(s) 75, 206
BstEII GGTNACC 1 cut(s) 1873
BstF5I GGATG 4 cut(s) 1853, 2342, 2707, 2958
BstFNI CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
BstH2I RGCGCY 1 cut(s) 227
BstHHI GCGC 9 cut(s) 20, 52, 58, 82, 100, 176, 226, 280, 1055
BstMAI GTCTC 4 cut(s) 1560, 1964, 2434, 3218
BstMCI CGRYCG 1 cut(s) 329
BstNI CCWGG 5 cut(s) 25, 178, 1407, 2510, 2950
BstNSI RCATGY 2 cut(s) 1075, 1675
BstPAI GACNNNNGTC 1 cut(s) 1542
BstPI GGTNACC 1 cut(s) 1873
BstSFI CTRYAG 3 cut(s) 1590, 2268, 2409
BstSLI GKGCMC 1 cut(s) 566
BstUI CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
BstV2I GAAGAC 4 cut(s) 460, 2449, 2964, 3055
BstX2I RGATCY 4 cut(s) 375, 1420, 2051, 3068
BstXI CCANNNNNNTGG 1 cut(s) 1189
BstYI RGATCY 4 cut(s) 375, 1420, 2051, 3068
Bsu36I CCTNAGG 2 cut(s) 795, 1572
BsuRI GGCC 4 cut(s) 74, 479, 2492, 2513
BtgI CCRYGG 2 cut(s) 75, 206
BtrI CACGTC 2 cut(s) 679, 3200
BtsCI GGATG 4 cut(s) 1853, 2342, 2707, 2958
BtsI GCAGTG 6 cut(s) 75, 1654, 2803, 2991, 3156, 3216
BtsIMutI CAGTG 9 cut(s) 75, 1364, 1654, 1839, 2803, 2926, 2991, 3156, 3216
BtuMI TCGCGA 1 cut(s) 509
BveI ACCTGC 2 cut(s) 375, 2163
Cac8I GCNNGC 7 cut(s) 54, 166, 333, 386, 507, 566, 2707
CfoI GCGC 9 cut(s) 20, 52, 58, 82, 100, 176, 226, 280, 1055
Cfr10I RCCGGY 5 cut(s) 10, 255, 292, 523, 3245
Cfr13I GGNCC 9 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2491, 2853
Cfr42I CCGCGG 1 cut(s) 209
CseI GACGC 7 cut(s) 16, 74, 170, 387, 538, 1946, 3020
Csp6I GTAC 4 cut(s) 190, 199, 761, 2657
CviQI GTAC 4 cut(s) 190, 199, 761, 2657
DrdI GACNNNNNNGTC 2 cut(s) 1929, 3098
DseDI GACNNNNNNGTC 2 cut(s) 1929, 3098
EaeI YGGCCR 1 cut(s) 72
Eam1104I CTCTTC 6 cut(s) 822, 861, 1150, 1594, 2044, 2877
EarI CTCTTC 6 cut(s) 822, 861, 1150, 1594, 2044, 2877
EciI GGCGGA 1 cut(s) 715
Ecl136II GAGCTC 1 cut(s) 418
Eco24I GRGCYC 2 cut(s) 420, 1580
Eco31I GGTCTC 2 cut(s) 1560, 2434
Eco32I GATATC 1 cut(s) 1495
Eco47I GGWCC 8 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2853
Eco53kI GAGCTC 1 cut(s) 418
Eco57I CTGAAG 4 cut(s) 428, 753, 1140, 2571
Eco81I CCTNAGG 2 cut(s) 795, 1572
Eco88I CYCGRG 1 cut(s) 634
Eco91I GGTNACC 1 cut(s) 1873
EcoICRI GAGCTC 1 cut(s) 418
EcoO109I RGGNCCY 3 cut(s) 1120, 1194, 2853
EcoO65I GGTNACC 1 cut(s) 1873
EcoRI GAATTC 1 cut(s) 1991
EcoRII CCWGG 5 cut(s) 23, 176, 1405, 2508, 2948
EcoRV GATATC 1 cut(s) 1495
EcoT22I ATGCAT 2 cut(s) 1909, 2002
EcoT38I GRGCYC 2 cut(s) 420, 1580
Esp3I CGTCTC 2 cut(s) 1964, 3218
FalI AAGNNNNNCTT 4 cut(s) 2949, 2981, 3047, 3079
FaqI GGGAC 6 cut(s) 689, 1133, 1485, 1917, 1935, 2491
FauI CCCGC 6 cut(s) 201, 528, 573, 610, 689, 1051
FbaI TGATCA 3 cut(s) 1354, 1758, 2968
FblI GTMKAC 2 cut(s) 269, 1170
FokI GGATG 4 cut(s) 1840, 2349, 2714, 2965
FriOI GRGCYC 2 cut(s) 420, 1580
FspBI CTAG 4 cut(s) 1368, 1914, 2298, 3297
GlaI GCGC 9 cut(s) 19, 51, 57, 81, 99, 175, 225, 279, 1054
GsaI CCCAGC 2 cut(s) 1554, 2427
GsuI CTGGAG 5 cut(s) 197, 248, 1040, 3104, 3163
HaeII RGCGCY 1 cut(s) 227
HaeIII GGCC 4 cut(s) 74, 479, 2492, 2513
HgaI GACGC 7 cut(s) 16, 74, 170, 387, 538, 1946, 3020
HhaI GCGC 9 cut(s) 20, 52, 58, 82, 100, 176, 226, 280, 1055
Hin1I GRCGYC 5 cut(s) 8, 107, 162, 398, 530
Hin6I GCGC 9 cut(s) 18, 50, 56, 80, 98, 174, 224, 278, 1053
HinP1I GCGC 9 cut(s) 18, 50, 56, 80, 98, 174, 224, 278, 1053
HincII GTYRAC 4 cut(s) 270, 934, 1520, 2226
HindII GTYRAC 4 cut(s) 270, 934, 1520, 2226
HindIII AAGCTT 2 cut(s) 1152, 2752
HphI GGTGA 7 cut(s) 509, 538, 1364, 1365, 1625, 1800, 1885
HpyCH4III ACNGT 4 cut(s) 1348, 1465, 2906, 2921
HpyCH4IV ACGT 3 cut(s) 107, 678, 3199
HpySE526I ACGT 3 cut(s) 107, 678, 3199
Hsp92I GRCGYC 5 cut(s) 8, 107, 162, 398, 530
HspAI GCGC 9 cut(s) 18, 50, 56, 80, 98, 174, 224, 278, 1053
Kpn2I TCCGGA 1 cut(s) 486
Ksp22I TGATCA 3 cut(s) 1354, 1758, 2968
KspI CCGCGG 1 cut(s) 209
LguI GCTCTTC 1 cut(s) 1150
LmnI GCTCC 8 cut(s) 90, 117, 180, 216, 229, 286, 489, 3251
LweI GCATC 9 cut(s) 970, 1574, 1714, 1916, 1987, 2200, 2327, 2692, 2855
MaeI CTAG 4 cut(s) 1368, 1914, 2298, 3297
MaeII ACGT 3 cut(s) 107, 678, 3199
MbiI CCGCTC 2 cut(s) 183, 696
MfeI CAATTG 2 cut(s) 1540, 1754
MflI RGATCY 4 cut(s) 375, 1420, 2051, 3068
MhlI GDGCHC 5 cut(s) 420, 566, 1364, 1580, 3256
MlyI GAGTC 9 cut(s) 469, 550, 587, 1176, 2342, 2531, 2918, 3108, 3268
MmeI TCCRAC 3 cut(s) 593, 1987, 2230
Mph1103I ATGCAT 2 cut(s) 1909, 2002
MroI TCCGGA 1 cut(s) 486
MroXI GAANNNNTTC 3 cut(s) 2999, 3062, 3171
MseI TTAA 5 cut(s) 2003, 2285, 2432, 2562, 3054
MslI CAYNNNNRTG 3 cut(s) 1076, 1871, 2726
MspA1I CMGCKG 5 cut(s) 208, 605, 731, 1611, 3082
MunI CAATTG 2 cut(s) 1540, 1754
MvaI CCWGG 5 cut(s) 25, 178, 1407, 2510, 2950
MvnI CGCG 8 cut(s) 52, 82, 98, 159, 174, 208, 509, 1055
NciI CCSGG 5 cut(s) 221, 353, 481, 2167, 3279
NmuCI GTSAC 6 cut(s) 494, 526, 1864, 1873, 2091, 2921
NruI TCGCGA 1 cut(s) 509
NsiI ATGCAT 2 cut(s) 1909, 2002
NspI RCATGY 2 cut(s) 1075, 1675
OliI CACNNNNGTG 1 cut(s) 1871
PaqCI CACCTGC 1 cut(s) 375
PciI ACATGT 1 cut(s) 1071
PciSI GCTCTTC 1 cut(s) 1150
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 3 cut(s) 2999, 3062, 3171
PflFI GACNNNGTC 2 cut(s) 106, 397
PleI GAGTC 9 cut(s) 468, 550, 587, 1175, 2342, 2531, 2918, 3107, 3268
PpsI GAGTC 9 cut(s) 468, 550, 587, 1175, 2342, 2531, 2918, 3107, 3268
PpuMI RGGWCCY 3 cut(s) 1120, 1194, 2853
PscI ACATGT 1 cut(s) 1071
PshAI GACNNNNGTC 1 cut(s) 1542
Psp124BI GAGCTC 1 cut(s) 420
Psp5II RGGWCCY 3 cut(s) 1120, 1194, 2853
Psp6I CCWGG 5 cut(s) 23, 176, 1405, 2508, 2948
PspEI GGTNACC 1 cut(s) 1873
PspFI CCCAGC 2 cut(s) 1550, 2423
PspGI CCWGG 5 cut(s) 23, 176, 1405, 2508, 2948
PspPI GGNCC 9 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2491, 2853
PspPPI RGGWCCY 3 cut(s) 1120, 1194, 2853
PstI CTGCAG 1 cut(s) 1594
PsuI RGATCY 4 cut(s) 375, 1420, 2051, 3068
PsyI GACNNNGTC 2 cut(s) 106, 397
PvuII CAGCTG 1 cut(s) 3082
RruI TCGCGA 1 cut(s) 509
RsaI GTAC 4 cut(s) 191, 200, 762, 2658
RsaNI GTAC 4 cut(s) 190, 199, 761, 2657
RseI CAYNNNNRTG 3 cut(s) 1076, 1871, 2726
SacI GAGCTC 1 cut(s) 420
SacII CCGCGG 1 cut(s) 209
SalI GTCGAC 1 cut(s) 268
SapI GCTCTTC 1 cut(s) 1150
SaqAI TTAA 5 cut(s) 2003, 2285, 2432, 2562, 3054
Sau96I GGNCC 9 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2491, 2853
SchI GAGTC 9 cut(s) 469, 550, 587, 1176, 2342, 2531, 2918, 3108, 3268
SduI GDGCHC 5 cut(s) 420, 566, 1364, 1580, 3256
SfaNI GCATC 9 cut(s) 970, 1574, 1714, 1916, 1987, 2200, 2327, 2692, 2855
SfcI CTRYAG 3 cut(s) 1590, 2268, 2409
Sfr303I CCGCGG 1 cut(s) 209
SgrAI CRCCGGYG 2 cut(s) 292, 523
SgrBI CCGCGG 1 cut(s) 209
SgrDI CGTCGACG 1 cut(s) 268
SinI GGWCC 8 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2853
SmiMI CAYNNNNRTG 3 cut(s) 1076, 1871, 2726
SmlI CTYRAG 1 cut(s) 419
SmoI CTYRAG 1 cut(s) 419
SspI AATATT 1 cut(s) 2695
SspMI CTAG 4 cut(s) 1368, 1914, 2298, 3297
SstI GAGCTC 1 cut(s) 420
TaaI ACNGT 4 cut(s) 1348, 1465, 2906, 2921
TaiI ACGT 3 cut(s) 110, 681, 3202
TaqI TCGA 9 cut(s) 269, 299, 414, 444, 642, 1107, 1161, 1995, 3009
Tru1I TTAA 5 cut(s) 2003, 2285, 2432, 2562, 3054
Tru9I TTAA 5 cut(s) 2003, 2285, 2432, 2562, 3054
TscAI CASTG 9 cut(s) 75, 1364, 1661, 1846, 2803, 2926, 2991, 3156, 3223
TseFI GTSAC 6 cut(s) 494, 526, 1864, 1873, 2091, 2921
Tsp45I GTSAC 6 cut(s) 494, 526, 1864, 1873, 2091, 2921
TspGWI ACGGA 4 cut(s) 53, 226, 605, 696
TspRI CASTG 9 cut(s) 75, 1364, 1661, 1846, 2803, 2926, 2991, 3156, 3223
Tth111I GACNNNGTC 2 cut(s) 106, 397
VpaK11BI GGWCC 8 cut(s) 253, 598, 1120, 1194, 1226, 1922, 2237, 2853
XapI RAATTY 4 cut(s) 1506, 1991, 2639, 3050
XceI RCATGY 2 cut(s) 1075, 1675
XmiI GTMKAC 2 cut(s) 269, 1170
XmnI GAANNNNTTC 3 cut(s) 2999, 3062, 3171
XspI CTAG 4 cut(s) 1368, 1914, 2298, 3297
ZraI GACGTC 1 cut(s) 108
Zsp2I ATGCAT 2 cut(s) 1909, 2002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.