pycom03g05500
ERF Family

Double Clp-N motif-containing P-loop nucleoside triphosphate hydrolases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010747
Physical Location & Seq
Reverse (-)
4331020 .. 4333683
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g05500.2

Sequence Viewer

Length: 2070 bp
ATGCCGACGCCGGTGAGCGCAGCGAGGCAATGCTTGACGGACGAGGCGGCACGTGCTTTGGACGACGCCGTTGTTGTAGCGCGCAGGCGGAGCCACGCCCAGACGACGTCGTTACATGCCGTCTCGGCCTTGCTTTCCTTGCCGTCCTCGGCCCTCCGCGACGCCTGCGCCCGCGCCAGGAGCAGCGCGTACTCCCCACGTCTACAGTTCCGGGCTCTCGAGCTCTCCGTCGGAGTTTCCTTGGACAGGTTGCCGTCGTCCAAGGCGCAGGATGAGCCTCCGGTCTCGAACTCGCTCATGGCGGCGATCAAGCGGTCTCAGGCGAACCAGAGGCGGCATCCGGAGAGCTTTCACTTGCACCAGATTCACAGCCAGCAGCAAGCGGCGTCCCTTTTGAAGGTTGAGCTGAAGCACTTTGTTTTATCTATTTTGGACGACCCGATTGTGAGTCGGGTCTTCGGGGAAGCCGGGTTTCGGAGCTGTGATATAAAGTTCGCGATTATTCACCCGCCGGTCACGCAATCCACCCGGTTTCCTCGGACCCGGTGCCCGCCCATTTTCCTGTGCAACTTAACGGACTCGGACCCTGCCCGACCCGGATTCAGCTTCCCTTTTTCCGGGTTCGAAGACAGAGACGAGAACTCCAGAAGAATCGCCGACGTGTTGGTTAGAAAGAGCGGGAAAAATCCGTTGCTAATCGGCGTTTGCGCCGGCGACGCTCTGAAAAGCTTCACCGAGGCCCTGCATAAGGGTAAAGCGGGGATTTTTCCAGCTGAAATCGATAACTTCAGCGTGGTATCCATTGAGAAGGAGGTTTCTGAGTTCGTCGTCAATGGCGGAAGCGAGGAGGAGATGGGTTGCAAGTTTGAGGAGGTGGGTCGAATGGCTGCGCGTTGCTCTGGAGCTGGAAGTGGTGTTATTGTAAATTTTGGGGATTTGAAGGGTTTAGTGGGTGAGGGTATGGTGGCGGAGGAAGCTCTGTGCTTTGTGGTGCTGCAGTTGAAGAACTTGCTGGAGATTCATGGTGGGAAATTACGGTTGATTGGCGCGGCTGCGAGCCACGAAGTGTTTACAAAGCTTTCGCTTTGGTTTTCGACGATTGAGAAGGATTGGGATCTTCATCTTCTTCCCATTACTTCTTCTAAAGCTTCAATGGAAGGAGTTTACTCGAAATCCAGTTTGATGGGGTCCTTCGTTCCATTTGGCGGGTTCTTTTCTGCACCGTCCAACTTCAAAAATCCATTGAGCAGCACATATCAATCGTTTAGACGTTGTAATGGGTGCACAGAGAAGTATGAGCAAGAAGTTGCTTCTGTTCTGAAAGTAGGATCAACTGTTTCAGTAACTGATCCGCGGTCAGATAGCTTGCCTTCTTGGTTACAAATACGCAAACTTGGCACTGGCAAGGGCGATGATCTAGCAAAGACCAAAGATGATAAAACAACAATGAATGTTACAGTATCAGCACTGCAGAAAAAATGGGATGATTTCTGCCGACAAAATCTTCACGCTCAACCATTCCCTAAAGTGGACATTTATCAAGCAGGGCGTCAAGTTGCATCTGCTGAGGGCTCCCTAGCCGTTTGGGATAGAAAAGAAAACAGTGGCGAGGACTCATCTCCAAATGAAAGTGGATGTGCTATTCAATTTCACTGCCAGCCGATGGACATGCAAACGAATTATCTATCAAAACAGAACTTGCCTGTGCAAGTAGTTTCTGATGCTGAGAATACCAGCTTCCAGTCCGAGCTATTGGTAAAAGATTCAAAGGGTCAGCGAGTTGAACTGGGAAGTCCTTGCCGAACTACTTACCCCATCCACAATCTGCCCACCAACTGCACATCTTCTGCGTTGGTCACTTCTGTGGCAACAGATTTGGGATTGGGAACATTATATGCAGCAACCAGCCAAGGGCCAATAAGTCCTCAATTACAAGATATTAAAGGGAGTTCCCGCCACCTCTCTGGTTCCATTTCTGCTGAATTTGATGCATTGAGTGAAAATAGTTCACACCAAATAGCTCAGTCCTCCTCCTGCTCTGCTTCTGATCTTCGCCGGAGGGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0009719 GO:0009725 GO:0010033 GO:0014070 GO:0033993 GO:0042221 GO:0050896 GO:1901700 GO:1902347
KEGG Pathways
Metabolic & Signaling
ko04213 map04213
Pfam Domains
Protein Families
AAA_2 Clp_N

Protein Analysis

690

Amino Acids

74.77

Weight (kDa)

7.23

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010747)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07200 AT1G07200 AT2G29970
fragaria_vesca FvH4_3g38740
malus_domestica MD03G1068500.v1.1 MD11G1072900.v1.1
prunus_persica Prupe.6G056400_v2.0.a1
pyrus_communis pycom03g05500 pycom11g06060
rosa_chinensis RchiOBHm_Chr5g0069891
rosa_laevigata RLG00000036105
rosa_multiflora Rmu_co8209630.1_g000001 Rmu_sc0030143.1_g000001
rosa_roxburghii Rroxscaffold_1G00011170
rosa_rugosa Rorug05G0400500
rosa_samantha Rh5AG457600 Rh5BG476400 Rh5CG500200 Rh5DG488300
rosa_wichuraiana Rw5G042720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 110
AccB1I GGYRCC 1 cut(s) 546
AccB7I CCANNNNNTGG 1 cut(s) 1663
AccBSI CCGCTC 1 cut(s) 678
AccI GTMKAC 1 cut(s) 202
AccII CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
AccIII TCCGGA 1 cut(s) 340
AclWI GGATC 3 cut(s) 1122, 1336, 1343
AcsI RAATTY 2 cut(s) 925, 1982
AcuI CTGAAG 2 cut(s) 428, 772
AcvI CACGTG 1 cut(s) 53
AcyI GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
AdeI CACNNNGTG 1 cut(s) 1066
AfaI GTAC 1 cut(s) 191
AflIII ACRYGT 1 cut(s) 660
AgsI TTSAA 8 cut(s) 397, 940, 1003, 1152, 1234, 1646, 1767, 1784
AjiI CACGTC 2 cut(s) 200, 661
AjnI CCWGG 1 cut(s) 176
AleI CACNNNNGTG 1 cut(s) 1862
Alw21I GWGCWC 2 cut(s) 225, 1286
Alw26I GTCTC 4 cut(s) 127, 289, 321, 627
Alw44I GTGCAC 1 cut(s) 1282
AlwI GGATC 3 cut(s) 1122, 1336, 1343
AlwNI CAGNNNCTG 1 cut(s) 1346
Ama87I CYCGRG 1 cut(s) 218
Aor13HI TCCGGA 1 cut(s) 340
AoxI GGCC 4 cut(s) 126, 150, 738, 1913
ApaLI GTGCAC 1 cut(s) 1282
ApeKI GCWGC 8 cut(s) 20, 183, 376, 887, 994, 1052, 1248, 1898
ApoI RAATTY 2 cut(s) 925, 1982
Asp700I GAANNNNTTC 1 cut(s) 728
AspS9I GGNCC 6 cut(s) 151, 540, 583, 739, 1188, 1913
AsuC2I CCSGG 6 cut(s) 212, 469, 529, 544, 597, 619
AsuHPI GGTGA 4 cut(s) 25, 497, 724, 965
AsuII TTCGAA 1 cut(s) 624
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 3 cut(s) 540, 583, 1188
BaeGI GKGCMC 2 cut(s) 551, 1286
BanI GGYRCC 1 cut(s) 546
BanII GRGCYC 3 cut(s) 217, 225, 1574
BbrPI CACGTG 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 448, 633
Bbv12I GWGCWC 2 cut(s) 225, 1286
BbvCI CCTCAGC 1 cut(s) 1566
BbvI GCAGC 8 cut(s) 32, 195, 388, 874, 981, 1039, 1260, 1910
BccI CCATC 4 cut(s) 847, 1177, 1657, 1823
BceAI ACGGC 5 cut(s) 53, 104, 127, 238, 1565
BcgI CGANNNNNNTGC 2 cut(s) 1651, 1685
BciT130I CCWGG 1 cut(s) 178
BciVI GTATCC 1 cut(s) 808
BcnI CCSGG 6 cut(s) 212, 469, 529, 544, 597, 619
BcoDI GTCTC 4 cut(s) 127, 289, 321, 627
BfaI CTAG 2 cut(s) 1418, 1577
BfmI CTRYAG 3 cut(s) 203, 995, 1469
BfuI GTATCC 1 cut(s) 808
BglI GCCNNNNNGGC 1 cut(s) 125
Bme1390I CCNGG 7 cut(s) 178, 212, 469, 529, 544, 597, 619
Bme18I GGWCC 3 cut(s) 540, 583, 1188
BmeT110I CYCGRG 1 cut(s) 218
BmgBI CACGTC 2 cut(s) 200, 661
BmgT120I GGNCC 6 cut(s) 151, 540, 583, 739, 1188, 1913
BmiI GGNNCC 7 cut(s) 92, 542, 548, 585, 1189, 1573, 1969
BmrFI CCNGG 7 cut(s) 178, 212, 469, 529, 544, 597, 619
BmrI ACTGGG 1 cut(s) 1796
BmsI GCATC 4 cut(s) 346, 1568, 1711, 1978
BmuI ACTGGG 1 cut(s) 1796
BpiI GAAGAC 2 cut(s) 448, 633
BpmI CTGGAG 3 cut(s) 628, 921, 1034
Bpu10I CCTNAGC 1 cut(s) 1566
Bpu14I TTCGAA 1 cut(s) 624
BpuMI CCSGG 6 cut(s) 212, 469, 529, 544, 597, 619
Bsa29I ATCGAT 1 cut(s) 780
BsaAI YACGTR 1 cut(s) 53
BsaBI GATNNNNATC 2 cut(s) 1113, 1119
BsaHI GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
BsaI GGTCTC 2 cut(s) 289, 321
BsaJI CCNNGG 7 cut(s) 147, 240, 261, 536, 735, 1352, 1909
BsaWI WCCGGW 2 cut(s) 280, 340
BsaXI ACNNNNNCTCC 2 cut(s) 626, 656
Bse118I RCCGGY 3 cut(s) 10, 511, 710
Bse1I ACTGG 4 cut(s) 1176, 1405, 1741, 1791
Bse3DI GCAATG 1 cut(s) 35
Bse8I GATNNNNATC 2 cut(s) 1113, 1119
BseAI TCCGGA 1 cut(s) 340
BseBI CCWGG 1 cut(s) 178
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 7 cut(s) 147, 240, 261, 536, 735, 1352, 1909
BseGI GGATG 5 cut(s) 277, 337, 1489, 1640, 1815
BseJI GATNNNNATC 2 cut(s) 1113, 1119
BseMI GCAATG 1 cut(s) 35
BseMII CTCAG 5 cut(s) 332, 810, 1557, 1716, 2036
BseNI ACTGG 4 cut(s) 1176, 1405, 1741, 1791
BsePI GCGCGC 1 cut(s) 80
BseRI GAGGAG 4 cut(s) 860, 863, 884, 2020
BseSI GKGCMC 2 cut(s) 551, 1286
BseXI GCAGC 8 cut(s) 32, 195, 388, 874, 981, 1039, 1260, 1910
BsgI GTGCAG 2 cut(s) 1203, 1822
Bsh1236I CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
BshFI GGCC 4 cut(s) 128, 152, 740, 1915
BshNI GGYRCC 1 cut(s) 546
BshVI ATCGAT 1 cut(s) 780
BsiHKAI GWGCWC 2 cut(s) 225, 1286
BsiHKCI CYCGRG 1 cut(s) 218
BslFI GGGAC 1 cut(s) 373
BsmAI GTCTC 4 cut(s) 127, 289, 321, 627
BsmBI CGTCTC 2 cut(s) 127, 627
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 4 cut(s) 128, 152, 740, 1915
Bso31I GGTCTC 2 cut(s) 289, 321
BsoBI CYCGRG 1 cut(s) 218
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
Bsp13I TCCGGA 1 cut(s) 340
Bsp143I GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
Bsp68I TCGCGA 1 cut(s) 497
BspANI GGCC 4 cut(s) 128, 152, 740, 1915
BspCNI CTCAG 5 cut(s) 331, 811, 1558, 1717, 2035
BspDI ATCGAT 1 cut(s) 780
BspEI TCCGGA 1 cut(s) 340
BspFNI CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
BspLI GGNNCC 7 cut(s) 92, 542, 548, 585, 1189, 1573, 1969
BspMAI CTGCAG 2 cut(s) 999, 1473
BspPI GGATC 3 cut(s) 1122, 1336, 1343
BspT104I TTCGAA 1 cut(s) 624
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 2 cut(s) 289, 321
BsrBI CCGCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 35
BsrFI RCCGGY 3 cut(s) 10, 511, 710
BsrI ACTGG 4 cut(s) 1176, 1405, 1741, 1791
BssAI RCCGGY 3 cut(s) 10, 511, 710
BssECI CCNNGG 7 cut(s) 147, 240, 261, 536, 735, 1352, 1909
BssHII GCGCGC 1 cut(s) 80
BssMI GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
BssNI GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
BssT1I CCWWGG 3 cut(s) 240, 261, 1909
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 6 cut(s) 207, 1038, 1224, 1336, 1459, 1604
BstACI GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
BstBAI YACGTR 1 cut(s) 53
BstBI TTCGAA 1 cut(s) 624
BstDEI CTNAG 5 cut(s) 318, 819, 1566, 1725, 2022
BstDSI CCRYGG 1 cut(s) 1352
BstENI CCTNNNNNAGG 3 cut(s) 244, 395, 746
BstF5I GGATG 5 cut(s) 277, 337, 1489, 1640, 1815
BstFNI CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
BstKTI GATC 6 cut(s) 309, 1117, 1331, 1351, 1417, 2050
BstMAI GTCTC 4 cut(s) 127, 289, 321, 627
BstMBI GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 2 cut(s) 119, 1672
BstSCI CCNGG 7 cut(s) 176, 210, 467, 527, 542, 595, 617
BstSFI CTRYAG 3 cut(s) 203, 995, 1469
BstSLI GKGCMC 2 cut(s) 551, 1286
BstUI CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
BstV1I GCAGC 8 cut(s) 32, 195, 388, 874, 981, 1039, 1260, 1910
BstV2I GAAGAC 2 cut(s) 448, 633
BstX2I RGATCY 1 cut(s) 1114
BstXI CCANNNNNNTGG 2 cut(s) 1183, 1964
BstYI RGATCY 1 cut(s) 1114
Bsu15I ATCGAT 1 cut(s) 780
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 4 cut(s) 128, 152, 740, 1915
BsuTUI ATCGAT 1 cut(s) 780
BtgI CCRYGG 1 cut(s) 1352
BtgZI GCGATG 1 cut(s) 1425
BtrI CACGTC 2 cut(s) 200, 661
BtsCI GGATG 5 cut(s) 277, 337, 1489, 1640, 1815
BtsI GCAGTG 2 cut(s) 1466, 1651
BtsIMutI CAGTG 4 cut(s) 1398, 1466, 1609, 1651
BtuMI TCGCGA 1 cut(s) 497
CaiI CAGNNNCTG 1 cut(s) 1346
Cfr10I RCCGGY 3 cut(s) 10, 511, 710
Cfr13I GGNCC 6 cut(s) 151, 540, 583, 739, 1188, 1913
Cfr42I CCGCGG 1 cut(s) 1355
ClaI ATCGAT 1 cut(s) 780
CseI GACGC 6 cut(s) 16, 74, 170, 375, 725, 1538
Csp6I GTAC 1 cut(s) 190
CviAII CATG 4 cut(s) 116, 298, 1022, 1669
CviQI GTAC 1 cut(s) 190
DdeI CTNAG 5 cut(s) 318, 819, 1566, 1725, 2022
DpnI GATC 6 cut(s) 308, 1116, 1330, 1350, 1416, 2049
DpnII GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
DraIII CACNNNGTG 1 cut(s) 1066
EciI GGCGGA 3 cut(s) 103, 852, 983
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 3 cut(s) 240, 261, 1909
Eco24I GRGCYC 3 cut(s) 217, 225, 1574
Eco31I GGTCTC 2 cut(s) 289, 321
Eco47I GGWCC 3 cut(s) 540, 583, 1188
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 428, 772
Eco72I CACGTG 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 218
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 3 cut(s) 244, 395, 746
EcoO109I RGGNCCY 2 cut(s) 739, 1188
EcoRII CCWGG 1 cut(s) 176
EcoT14I CCWWGG 3 cut(s) 240, 261, 1909
EcoT22I ATGCAT 1 cut(s) 1993
EcoT38I GRGCYC 3 cut(s) 217, 225, 1574
ErhI CCWWGG 3 cut(s) 240, 261, 1909
Esp3I CGTCTC 2 cut(s) 127, 627
FaeI CATG 4 cut(s) 119, 301, 1025, 1672
FaqI GGGAC 1 cut(s) 373
FatI CATG 4 cut(s) 115, 297, 1021, 1668
FauI CCCGC 7 cut(s) 179, 516, 558, 671, 751, 1199, 1961
FblI GTMKAC 1 cut(s) 202
FokI GGATG 5 cut(s) 284, 324, 1496, 1647, 1802
FriOI GRGCYC 3 cut(s) 217, 225, 1574
FspBI CTAG 2 cut(s) 1418, 1577
GsuI CTGGAG 3 cut(s) 628, 921, 1034
HaeIII GGCC 4 cut(s) 128, 152, 740, 1915
HgaI GACGC 6 cut(s) 16, 74, 170, 375, 725, 1538
Hin1I GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
Hin1II CATG 4 cut(s) 119, 301, 1025, 1672
HindIII AAGCTT 3 cut(s) 727, 1076, 1146
HinfI GANTC 8 cut(s) 364, 448, 578, 600, 651, 1018, 1613, 1763
HphI GGTGA 4 cut(s) 25, 497, 724, 965
Hpy166II GTNNAC 6 cut(s) 203, 1071, 1165, 1284, 1531, 2009
Hpy188III TCNNGA 6 cut(s) 218, 286, 341, 496, 645, 900
Hpy8I GTNNAC 6 cut(s) 203, 1071, 1165, 1284, 1531, 2009
HpyAV CCTTC 7 cut(s) 391, 802, 934, 1099, 1151, 1201, 1380
HpyCH4III ACNGT 6 cut(s) 207, 1038, 1224, 1336, 1459, 1604
HpyCH4IV ACGT 5 cut(s) 52, 107, 199, 660, 1270
HpyF3I CTNAG 5 cut(s) 318, 819, 1566, 1725, 2022
HpySE526I ACGT 5 cut(s) 52, 107, 199, 660, 1270
Hsp92I GRCGYC 6 cut(s) 8, 66, 107, 162, 386, 1549
Hsp92II CATG 4 cut(s) 119, 301, 1025, 1672
Kpn2I TCCGGA 1 cut(s) 340
KroI GCCGGC 1 cut(s) 710
KroNI GCCGGC 1 cut(s) 712
KspI CCGCGG 1 cut(s) 1355
Kzo9I GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
LmnI GCTCC 5 cut(s) 90, 180, 477, 902, 1577
Lsp1109I GCAGC 8 cut(s) 32, 195, 388, 874, 981, 1039, 1260, 1910
LweI GCATC 4 cut(s) 346, 1568, 1711, 1978
MaeI CTAG 2 cut(s) 1418, 1577
MaeII ACGT 5 cut(s) 52, 107, 199, 660, 1270
MaeIII GTNAC 6 cut(s) 111, 514, 1342, 1377, 1453, 1855
MalI GATC 6 cut(s) 308, 1116, 1330, 1350, 1416, 2049
MbiI CCGCTC 1 cut(s) 678
MboI GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
MflI RGATCY 1 cut(s) 1114
MhlI GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
MluCI AATT 6 cut(s) 925, 1031, 1646, 1678, 1928, 1982
MlyI GAGTC 3 cut(s) 457, 572, 1607
MmeI TCCRAC 2 cut(s) 211, 1251
Mph1103I ATGCAT 1 cut(s) 1993
MreI CGCCGGCG 1 cut(s) 710
MroI TCCGGA 1 cut(s) 340
MroNI GCCGGC 1 cut(s) 710
MroXI GAANNNNTTC 1 cut(s) 728
MseI TTAA 2 cut(s) 572, 1941
MslI CAYNNNNRTG 1 cut(s) 1862
MspA1I CMGCKG 2 cut(s) 773, 1354
MspR9I CCNGG 7 cut(s) 178, 212, 469, 529, 544, 597, 619
MvaI CCWGG 1 cut(s) 178
MvnI CGCG 8 cut(s) 82, 159, 174, 188, 497, 892, 1049, 1354
NaeI GCCGGC 1 cut(s) 712
NciI CCSGG 6 cut(s) 212, 469, 529, 544, 597, 619
NdeII GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
NgoMIV GCCGGC 1 cut(s) 710
NlaIII CATG 4 cut(s) 119, 301, 1025, 1672
NlaIV GGNNCC 7 cut(s) 92, 542, 548, 585, 1189, 1573, 1969
NmeAIII GCCGAG 2 cut(s) 104, 128
NmuCI GTSAC 2 cut(s) 514, 1855
NruI TCGCGA 1 cut(s) 497
NsiI ATGCAT 1 cut(s) 1993
NspI RCATGY 2 cut(s) 119, 1672
NspV TTCGAA 1 cut(s) 624
OliI CACNNNNGTG 1 cut(s) 1862
PaeR7I CTCGAG 1 cut(s) 218
PauI GCGCGC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdiI GCCGGC 1 cut(s) 712
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 5 cut(s) 364, 600, 651, 1018, 1763
PflFI GACNNNGTC 1 cut(s) 106
PflMI CCANNNNNTGG 1 cut(s) 1663
PleI GAGTC 3 cut(s) 456, 572, 1607
PmaCI CACGTG 1 cut(s) 53
PmlI CACGTG 1 cut(s) 53
PpsI GAGTC 3 cut(s) 456, 572, 1607
Ppu21I YACGTR 1 cut(s) 53
PpuMI RGGWCCY 1 cut(s) 1188
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 1188
Psp6I CCWGG 1 cut(s) 176
PspCI CACGTG 1 cut(s) 53
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 7 cut(s) 92, 542, 548, 585, 1189, 1573, 1969
PspPI GGNCC 6 cut(s) 151, 540, 583, 739, 1188, 1913
PspPPI RGGWCCY 1 cut(s) 1188
PstI CTGCAG 2 cut(s) 999, 1473
PstNI CAGNNNCTG 1 cut(s) 1346
PsuI RGATCY 1 cut(s) 1114
PsyI GACNNNGTC 1 cut(s) 106
PteI GCGCGC 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 773
RruI TCGCGA 1 cut(s) 497
RsaI GTAC 1 cut(s) 191
RsaNI GTAC 1 cut(s) 190
RseI CAYNNNNRTG 1 cut(s) 1862
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 1355
SaqAI TTAA 2 cut(s) 572, 1941
Sau3AI GATC 6 cut(s) 306, 1114, 1328, 1348, 1414, 2047
Sau96I GGNCC 6 cut(s) 151, 540, 583, 739, 1188, 1913
SchI GAGTC 3 cut(s) 457, 572, 1607
ScrFI CCNGG 7 cut(s) 178, 212, 469, 529, 544, 597, 619
SduI GDGCHC 5 cut(s) 217, 225, 551, 1286, 1574
SfaNI GCATC 4 cut(s) 346, 1568, 1711, 1978
SfcI CTRYAG 3 cut(s) 203, 995, 1469
Sfr274I CTCGAG 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 1355
SfuI TTCGAA 1 cut(s) 624
SgrAI CRCCGGYG 2 cut(s) 10, 710
SgrBI CCGCGG 1 cut(s) 1355
SinI GGWCC 3 cut(s) 540, 583, 1188
SlaI CTCGAG 1 cut(s) 218
SmiMI CAYNNNNRTG 1 cut(s) 1862
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 6 cut(s) 925, 1031, 1646, 1678, 1928, 1982
SspMI CTAG 2 cut(s) 1418, 1577
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 7 cut(s) 176, 210, 467, 527, 542, 595, 617
StyI CCWWGG 3 cut(s) 240, 261, 1909
TaaI ACNGT 6 cut(s) 207, 1038, 1224, 1336, 1459, 1604
TaiI ACGT 5 cut(s) 55, 110, 202, 663, 1273
TaqI TCGA 7 cut(s) 219, 287, 624, 780, 880, 1094, 1169
TasI AATT 6 cut(s) 925, 1031, 1646, 1678, 1928, 1982
TauI GCSGC 5 cut(s) 50, 305, 337, 386, 1052
TfiI GAWTC 5 cut(s) 364, 600, 651, 1018, 1763
Tru1I TTAA 2 cut(s) 572, 1941
Tru9I TTAA 2 cut(s) 572, 1941
TscAI CASTG 4 cut(s) 1405, 1473, 1609, 1658
TseFI GTSAC 2 cut(s) 514, 1855
TseI GCWGC 8 cut(s) 20, 183, 376, 887, 994, 1052, 1248, 1898
Tsp45I GTSAC 2 cut(s) 514, 1855
TspDTI ATGAA 4 cut(s) 1010, 1109, 1463, 1641
TspGWI ACGGA 4 cut(s) 53, 217, 590, 678
TspRI CASTG 4 cut(s) 1405, 1473, 1609, 1658
Tth111I GACNNNGTC 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 1663
VneI GTGCAC 1 cut(s) 1282
VpaK11BI GGWCC 3 cut(s) 540, 583, 1188
XagI CCTNNNNNAGG 3 cut(s) 244, 395, 746
XapI RAATTY 2 cut(s) 925, 1982
XceI RCATGY 2 cut(s) 119, 1672
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 2 cut(s) 1418, 1577
ZraI GACGTC 1 cut(s) 108
Zsp2I ATGCAT 1 cut(s) 1993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.