MD03G1142000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012214
Physical Location & Seq
Forward (+)
15114546 .. 15118108
3563 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1142000.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGGAAAGCGCGATAAGAAACCGAGACACCAGCAAAGGAGAGCTGCCTTCCACTACACTGAGGAAGACGATGGCGACGGTGATTATGAAGGTCACTCCGCTTCCAAGCTCACTGTTTCCGAAGAAGAAGAGGAGGAGGAGGAGGCGGCGGCGGAGGAGGAGGTTGCCGGAGAAGAGGTTGCTGATGAATACCCATCAACTGATATGCCCTCAAAGTTTCTTCTTTATCAACAATCTGTACAGTCGCCGAAAGGAGACATAAGCTATTTGCAGAAGTTCTTTTTGATGTATGTGGGTGGGAGGTTACCTCTCCATCTCCAAGAAGATTTCTGTGGTACTGCACTGCTTAGTGCAGAATGGCTCCGGACTGACCCGAGAAGGACTGCTGTAGGGTTGGATTTGGACCCTGAAGCGCTAAACTGGTGCTTGGAGAACAACATAAATAAAGTTGGGTCTGATGGATATTCCAGAATATCCCTCTTTCACGGGAATGTCTTACAACCTCTTGAGGCCAAACTAATTAAGTCTGAGGCTGAGGAGCAGATGAGAAACATTTCACTCAGTGAGAACGGAGATGGTTTGGAGAGTGATGGATTGGAATCTACTCTTCAAGAGAGCTCTTCTTCCTCACATGATGAGAAGTACTTAAGCAACCATCCGTTGCCTGCTAGAGACATTGTGTGTGCTTTTAACTACAGCTGTTGTTGTCTCCATCAACGTGCTGAACTGGTTCTGTATCTCAAGCATGCTCTTGCTGTCTTGTCAAAGAAAGGTGGAATATTTGTAATGGATTTGTACGGCGGTACATCCTCAGAGTGCAAGCTAAGGCTTCAAAGAAGATTTGCCAATTTTACGTATGTTTGGGAGCAAGCTGAATTTGATATAATTGAGCGCAAGACAAGAATCAGCCTCCATTTCCATCTCAAAAAACAGCAGAAGAAAATTCGACATGCATTTTCATACAGCTGGAGGCTGTGGTCGCTGCCGGAGATCAAGGACTGCATGGAAGATGCAGGCTTTAAGTCTGTCCATTTTTGGCTGAGGAAGATGCAAGACAGCGGAGAAATCCGAAGAACAGAGGGTTTTGGTGTTGGGCGAGACATAAAATATGAAGAGGTGAAACATTTTCAGCAACATGATTCATGGAATGCATACATCGTAGGTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

391

Amino Acids

44.78

Weight (kDa)

5.4

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27590
fragaria_vesca FvH4_3g24630 FvH4_3g24630
malus_domestica MD03G1142000.v1.1 MD11G1162600.v1.1
prunus_persica Prupe.6G127300_v2.0.a1 Prupe.6G127300_v2.0.a1
pyrus_communis pycom03g09680
rosa_chinensis RchiOBHm_Chr5g0044201
rosa_laevigata RLG00000034252
rosa_multiflora Rmu_sc0000954.1_g000008 Rmu_sc0019416.1_g000001
rosa_roxburghii Rroxscaffold_1G00036840
rosa_rugosa Rorug05G0211500 Rorug05G0211600.1
rosa_samantha Rh5BG304000 Rh5CG331100 Rh5DG312700
rosa_wichuraiana Rw5G027450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 363
AciI CCGC 6 cut(s) 99, 146, 149, 152, 801, 1059
AcsI RAATTY 2 cut(s) 875, 942
AcuI CTGAAG 1 cut(s) 429
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 5 cut(s) 240, 337, 644, 797, 805
AfeI AGCGCT 1 cut(s) 414
AflII CTTAAG 1 cut(s) 646
AgsI TTSAA 2 cut(s) 611, 833
AluBI AGCT 8 cut(s) 44, 109, 264, 618, 699, 823, 872, 966
AluI AGCT 8 cut(s) 44, 109, 264, 618, 699, 823, 872, 966
Alw21I GWGCWC 1 cut(s) 620
Alw26I GTCTC 5 cut(s) 19, 249, 666, 713, 1092
Ama87I CYCGRG 1 cut(s) 373
Aor13HI TCCGGA 1 cut(s) 363
Aor51HI AGCGCT 1 cut(s) 414
AoxI GGCC 1 cut(s) 510
ApeKI GCWGC 2 cut(s) 44, 982
ApoI RAATTY 2 cut(s) 875, 942
Asp700I GAANNNNTTC 2 cut(s) 553, 729
AspLEI GCGC 3 cut(s) 12, 415, 894
AspS9I GGNCC 1 cut(s) 403
AsuHPI GGTGA 2 cut(s) 92, 1129
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 1 cut(s) 403
BanII GRGCYC 1 cut(s) 620
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 620
BbvCI CCTCAGC 2 cut(s) 534, 1040
BbvI GCAGC 2 cut(s) 31, 969
BccI CCATC 9 cut(s) 65, 202, 321, 452, 569, 584, 663, 720, 927
BceAI ACGGC 1 cut(s) 814
BcoDI GTCTC 5 cut(s) 19, 249, 666, 713, 1092
BfaI CTAG 1 cut(s) 669
BfmI CTRYAG 2 cut(s) 387, 694
BfoI RGCGCY 1 cut(s) 416
BfrI CTTAAG 1 cut(s) 646
BisI GCNGC 4 cut(s) 45, 147, 150, 983
BlsI GCNGC 4 cut(s) 46, 148, 151, 984
BmcAI AGTACT 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 403
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 2 cut(s) 362, 405
BmsI GCATC 2 cut(s) 1000, 1038
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 292, 324
BpmI CTGGAG 1 cut(s) 988
Bpu10I CCTNAGC 3 cut(s) 534, 824, 1040
BpuEI CTTGAG 2 cut(s) 527, 725
BsaAI YACGTR 1 cut(s) 855
BsaBI GATNNNNATC 1 cut(s) 598
BsaWI WCCGGW 1 cut(s) 363
BsaXI ACNNNNNCTCC 2 cut(s) 162, 192
Bse1I ACTGG 2 cut(s) 425, 732
Bse8I GATNNNNATC 1 cut(s) 598
BseAI TCCGGA 1 cut(s) 363
BseGI GGATG 2 cut(s) 655, 806
BseJI GATNNNNATC 1 cut(s) 598
BseMII CTCAG 6 cut(s) 51, 519, 525, 574, 825, 1031
BseNI ACTGG 2 cut(s) 425, 732
BseRI GAGGAG 7 cut(s) 146, 149, 152, 155, 170, 173, 551
BseXI GCAGC 2 cut(s) 31, 969
BsgI GTGCAG 2 cut(s) 324, 372
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 512
BsiHKAI GWGCWC 1 cut(s) 620
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 3 cut(s) 168, 364, 986
BsmAI GTCTC 5 cut(s) 19, 249, 666, 713, 1092
BsmI GAATGC 1 cut(s) 1153
BsnI GGCC 1 cut(s) 512
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 1 cut(s) 620
Bsp13I TCCGGA 1 cut(s) 363
Bsp1407I TGTACA 1 cut(s) 238
Bsp143I GATC 1 cut(s) 990
BspACI CCGC 6 cut(s) 99, 146, 149, 152, 801, 1059
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 6 cut(s) 52, 520, 526, 573, 824, 1032
BspEI TCCGGA 1 cut(s) 363
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 362, 405
BspQI GCTCTTC 1 cut(s) 625
BspTI CTTAAG 1 cut(s) 646
BsrGI TGTACA 1 cut(s) 238
BsrI ACTGG 2 cut(s) 425, 732
BssMI GATC 1 cut(s) 990
Bst4CI ACNGT 3 cut(s) 80, 115, 243
Bst6I CTCTTC 5 cut(s) 123, 168, 612, 625, 1107
BstAFI CTTAAG 1 cut(s) 646
BstAUI TGTACA 1 cut(s) 238
BstBAI YACGTR 1 cut(s) 855
BstC8I GCNNGC 5 cut(s) 666, 747, 821, 870, 1015
BstDEI CTNAG 8 cut(s) 60, 347, 528, 534, 560, 811, 824, 1040
BstEII GGTNACC 1 cut(s) 303
BstF5I GGATG 2 cut(s) 655, 806
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 416
BstHHI GCGC 3 cut(s) 12, 415, 894
BstKTI GATC 1 cut(s) 993
BstMAI GTCTC 5 cut(s) 19, 249, 666, 713, 1092
BstMBI GATC 1 cut(s) 990
BstMWI GCNNNNNNNGC 1 cut(s) 979
BstNSI RCATGY 2 cut(s) 749, 953
BstPI GGTNACC 1 cut(s) 303
BstSFI CTRYAG 2 cut(s) 387, 694
BstSNI TACGTA 1 cut(s) 855
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 2 cut(s) 31, 969
BstV2I GAAGAC 1 cut(s) 72
BsuRI GGCC 1 cut(s) 512
BtsCI GGATG 2 cut(s) 655, 806
BtsI GCAGTG 1 cut(s) 341
BtsIMutI CAGTG 4 cut(s) 57, 111, 341, 568
Cac8I GCNNGC 5 cut(s) 666, 747, 821, 870, 1015
CfoI GCGC 3 cut(s) 12, 415, 894
Cfr13I GGNCC 1 cut(s) 403
Csp6I GTAC 5 cut(s) 239, 336, 643, 796, 804
CviAII CATG 6 cut(s) 632, 746, 950, 1003, 1136, 1143
CviQI GTAC 5 cut(s) 239, 336, 643, 796, 804
DdeI CTNAG 8 cut(s) 60, 347, 528, 534, 560, 811, 824, 1040
DpnI GATC 1 cut(s) 992
DpnII GATC 1 cut(s) 990
DraIII CACNNNGTG 1 cut(s) 563
Eam1104I CTCTTC 5 cut(s) 123, 168, 612, 625, 1107
EarI CTCTTC 5 cut(s) 123, 168, 612, 625, 1107
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 618
Eco105I TACGTA 1 cut(s) 855
Eco24I GRGCYC 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 403
Eco47III AGCGCT 1 cut(s) 414
Eco53kI GAGCTC 1 cut(s) 618
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 373
Eco91I GGTNACC 1 cut(s) 303
EcoICRI GAGCTC 1 cut(s) 618
EcoO65I GGTNACC 1 cut(s) 303
EcoT22I ATGCAT 2 cut(s) 955, 1153
EcoT38I GRGCYC 1 cut(s) 620
FaeI CATG 6 cut(s) 635, 749, 953, 1006, 1139, 1146
FatI CATG 6 cut(s) 631, 745, 949, 1002, 1135, 1142
Fnu4HI GCNGC 4 cut(s) 45, 147, 150, 983
FokI GGATG 2 cut(s) 642, 793
FriOI GRGCYC 1 cut(s) 620
Fsp4HI GCNGC 4 cut(s) 45, 147, 150, 983
FspBI CTAG 1 cut(s) 669
GlaI GCGC 3 cut(s) 11, 414, 893
GluI GCNGC 4 cut(s) 45, 147, 150, 983
GsuI CTGGAG 1 cut(s) 988
HaeII RGCGCY 1 cut(s) 416
HaeIII GGCC 1 cut(s) 512
HapII CCGG 3 cut(s) 168, 364, 986
HhaI GCGC 3 cut(s) 12, 415, 894
Hin1II CATG 6 cut(s) 635, 749, 953, 1006, 1139, 1146
Hin6I GCGC 3 cut(s) 10, 413, 892
HinP1I GCGC 3 cut(s) 10, 413, 892
HinfI GANTC 3 cut(s) 599, 903, 1139
HpaII CCGG 3 cut(s) 168, 364, 986
HphI GGTGA 2 cut(s) 92, 1129
Hpy188I TCNGA 5 cut(s) 121, 457, 529, 814, 1070
Hpy188III TCNNGA 4 cut(s) 364, 468, 506, 611
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 58, 83, 372
HpyCH4III ACNGT 3 cut(s) 80, 115, 243
HpyCH4IV ACGT 2 cut(s) 718, 854
HpyF10VI GCNNNNNNNGC 1 cut(s) 979
HpyF3I CTNAG 8 cut(s) 60, 347, 528, 534, 560, 811, 824, 1040
HpySE526I ACGT 2 cut(s) 718, 854
Hsp92II CATG 6 cut(s) 635, 749, 953, 1006, 1139, 1146
HspAI GCGC 3 cut(s) 10, 413, 892
Kpn2I TCCGGA 1 cut(s) 363
Kzo9I GATC 1 cut(s) 990
LguI GCTCTTC 1 cut(s) 625
LmnI GCTCC 3 cut(s) 366, 538, 865
Lsp1109I GCAGC 2 cut(s) 31, 969
LweI GCATC 2 cut(s) 1000, 1038
MaeI CTAG 1 cut(s) 669
MaeII ACGT 2 cut(s) 718, 854
MaeIII GTNAC 2 cut(s) 92, 303
MalI GATC 1 cut(s) 992
MboI GATC 1 cut(s) 990
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 5 cut(s) 519, 847, 875, 885, 942
MmeI TCCRAC 1 cut(s) 375
Mph1103I ATGCAT 2 cut(s) 955, 1153
MroI TCCGGA 1 cut(s) 363
MroXI GAANNNNTTC 2 cut(s) 553, 729
MseI TTAA 4 cut(s) 522, 647, 690, 1020
MslI CAYNNNNRTG 2 cut(s) 489, 717
MspA1I CMGCKG 3 cut(s) 699, 966, 1059
MspCI CTTAAG 1 cut(s) 646
MspI CCGG 3 cut(s) 168, 364, 986
Mva1269I GAATGC 1 cut(s) 1153
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 979
NdeII GATC 1 cut(s) 990
NlaIII CATG 6 cut(s) 635, 749, 953, 1006, 1139, 1146
NlaIV GGNNCC 2 cut(s) 362, 405
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 2 cut(s) 955, 1153
NspI RCATGY 2 cut(s) 749, 953
PaeI GCATGC 1 cut(s) 749
PciSI GCTCTTC 1 cut(s) 625
PctI GAATGC 1 cut(s) 1153
PdmI GAANNNNTTC 2 cut(s) 553, 729
PfeI GAWTC 3 cut(s) 599, 903, 1139
PkrI GCNGC 4 cut(s) 46, 148, 151, 984
Ppu21I YACGTR 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 620
PspEI GGTNACC 1 cut(s) 303
PspN4I GGNNCC 2 cut(s) 362, 405
PspPI GGNCC 1 cut(s) 403
PvuII CAGCTG 2 cut(s) 699, 966
RsaI GTAC 5 cut(s) 240, 337, 644, 797, 805
RsaNI GTAC 5 cut(s) 239, 336, 643, 796, 804
RseI CAYNNNNRTG 2 cut(s) 489, 717
SacI GAGCTC 1 cut(s) 620
SapI GCTCTTC 1 cut(s) 625
SaqAI TTAA 4 cut(s) 522, 647, 690, 1020
SatI GCNGC 4 cut(s) 45, 147, 150, 983
Sau3AI GATC 1 cut(s) 990
Sau96I GGNCC 1 cut(s) 403
ScaI AGTACT 1 cut(s) 644
SduI GDGCHC 1 cut(s) 620
SfaNI GCATC 2 cut(s) 1000, 1038
SfcI CTRYAG 2 cut(s) 387, 694
SinI GGWCC 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 489, 717
SmlI CTYRAG 3 cut(s) 506, 646, 740
SmoI CTYRAG 3 cut(s) 506, 646, 740
SnaBI TACGTA 1 cut(s) 855
SphI GCATGC 1 cut(s) 749
Sse9I AATT 5 cut(s) 519, 847, 875, 885, 942
SsiI CCGC 6 cut(s) 99, 146, 149, 152, 801, 1059
SspI AATATT 1 cut(s) 780
SspMI CTAG 1 cut(s) 669
SstI GAGCTC 1 cut(s) 620
TaaI ACNGT 3 cut(s) 80, 115, 243
TaiI ACGT 2 cut(s) 721, 857
TaqI TCGA 1 cut(s) 946
TasI AATT 5 cut(s) 519, 847, 875, 885, 942
TatI WGTACW 2 cut(s) 238, 642
TauI GCSGC 2 cut(s) 149, 152
TfiI GAWTC 3 cut(s) 599, 903, 1139
Tru1I TTAA 4 cut(s) 522, 647, 690, 1020
Tru9I TTAA 4 cut(s) 522, 647, 690, 1020
TscAI CASTG 4 cut(s) 64, 118, 348, 568
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 2 cut(s) 44, 982
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 5 cut(s) 102, 201, 948, 1125, 1131
TspGWI ACGGA 2 cut(s) 585, 648
TspRI CASTG 4 cut(s) 64, 118, 348, 568
Vha464I CTTAAG 1 cut(s) 646
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 2 cut(s) 875, 942
XceI RCATGY 2 cut(s) 749, 953
XmnI GAANNNNTTC 2 cut(s) 553, 729
XspI CTAG 1 cut(s) 669
ZrmI AGTACT 1 cut(s) 644
Zsp2I ATGCAT 2 cut(s) 955, 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.