Rh5DG312700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012214
Physical Location & Seq
Forward (+)
40097616 .. 40100071
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG312700.1

Sequence Viewer

Length: 1185 bp
ATGGGAAAGCGAGACAAGAAACAGAGACACCAACACAGAACCTCCCACAGAGCTGCCTTTCACTACACTGAAGAAGGTGAAGAAGGTGTCAGCTTTGAAAGTCATGCTGCTTCCAAGTTCAGTCTTTCTGAAGAAGAAGAAGAAGAAGAAGTAGAAAAAAAAGACGAAGTCGAAGACGAAGCAGAGATTGAGGAAACAGAGGAAACTGATAAATACCCATCAAAAGATATGCCCTCAAAGTTTCTTCTTTATCAACAATCTGTGCAGTCGCCAAAAGGAGACATAAGCTATTTGCAGAAGTTCTTTTTGATGTATGTGGGTGGGAGGCAACCTCTCCATCTCCAAGAAGATTTTTGTGGCACTGCTCTTCTTAGTGCAGAATGGCTTCGCACGGACTCTAGAAGGACTGCTGTAGGGTTGGATTTGGACCTTGAAGCGCTAGAATGGTGCATGGAGAACAACATAAATAAGATAGGGTCTGATGGGTATTCCAGAATATCCCTCTTCCATGGGAATGTCTTGCAACCTCTTGAGGCCAAGCTAATTGAGTTTGAGCCCCAGGAGCTGATAAGAAACATTTCACTCTCTGAAAACAAAGATGTTTCTGAGAGTGGTGGACTTGAAGGGGGTTCTTCTTCTCCACATAATGAGAAGTACATGAGGAACCATCCATTGCCTGCTAGAGACATAGTGTGTGCTTTTAACTACAGCTGTTGCTGTCTTCATCGACGTGCTGAACTGGTTTTGTATTTCAAGCATGTTCTTGATGTCTTGTCAAAGAAAGGTGGAATATTTGTAATGGATTTATATGGTGGTACATCATCAGAGTGCAAGTTAAGGCTTCAAAGGAAATTTGCCAATTTCACGTATTTTTGGGAGCAAGCTGAATTTGATATCATTGAGCGCAAAACAAGGATTAGTCTCCATTTCCATCTCAAAAAGCAGCAGAAGAAACTTCGACATGCATTTTCATACAGCTGGAGGCTGTGGTCATTGCCTGAGATTAAGGACTGCTTAGAAGAGGCAGGTTTTCAATCTGTCCACTTTTGGTTGCGCGAGATGCAAGACACCAGAGAAATCAGAAAAACAGAAGGTTTTGGTGTTGGGCGAGACATAAAATATGAAGAGGTTACAAAATTTCAGCAACAAGATGCTTGGAATGCATATGTCGTAGCTGTTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

45.93

Weight (kDa)

5.61

Isoelectric Point (pI)

66.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27590
fragaria_vesca FvH4_3g24630 FvH4_3g24630
malus_domestica MD03G1142000.v1.1 MD11G1162600.v1.1
prunus_persica Prupe.6G127300_v2.0.a1 Prupe.6G127300_v2.0.a1
pyrus_communis pycom03g09680
rosa_chinensis RchiOBHm_Chr5g0044201
rosa_laevigata RLG00000034252
rosa_multiflora Rmu_sc0000954.1_g000008 Rmu_sc0019416.1_g000001
rosa_roxburghii Rroxscaffold_1G00036840
rosa_rugosa Rorug05G0211500 Rorug05G0211600.1
rosa_samantha Rh5BG304000 Rh5CG331100 Rh5DG312700
rosa_wichuraiana Rw5G027450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1016
AccII CGCG 1 cut(s) 1056
AcsI RAATTY 3 cut(s) 851, 887, 1136
AcuI CTGAAG 2 cut(s) 90, 150
AfaI GTAC 2 cut(s) 656, 817
AfeI AGCGCT 1 cut(s) 438
AgsI TTSAA 6 cut(s) 98, 434, 623, 754, 845, 1034
AjiI CACGTC 1 cut(s) 731
AjnI CCWGG 1 cut(s) 558
AluBI AGCT 9 cut(s) 53, 93, 288, 541, 565, 711, 884, 978, 1175
AluI AGCT 9 cut(s) 53, 93, 288, 541, 565, 711, 884, 978, 1175
Alw26I GTCTC 6 cut(s) 6, 19, 273, 678, 926, 1104
AlwNI CAGNNNCTG 1 cut(s) 565
Aor51HI AGCGCT 1 cut(s) 438
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 3 cut(s) 53, 107, 943
ApoI RAATTY 3 cut(s) 851, 887, 1136
Asp700I GAANNNNTTC 2 cut(s) 384, 577
AspLEI GCGC 3 cut(s) 439, 906, 1056
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 558
BbsI GAAGAC 2 cut(s) 180, 713
BbvI GCAGC 3 cut(s) 40, 94, 955
BccI CCATC 5 cut(s) 226, 345, 476, 675, 939
BciT130I CCWGG 1 cut(s) 560
BcoDI GTCTC 6 cut(s) 6, 19, 273, 678, 926, 1104
BfaI CTAG 4 cut(s) 399, 440, 681, 1183
BfmI CTRYAG 2 cut(s) 411, 706
BfoI RGCGCY 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 1016
BisI GCNGC 3 cut(s) 54, 108, 944
BlsI GCNGC 3 cut(s) 55, 109, 945
Bme1390I CCNGG 1 cut(s) 560
Bme18I GGWCC 1 cut(s) 427
BmgBI CACGTC 1 cut(s) 731
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 1 cut(s) 665
BmrFI CCNGG 1 cut(s) 560
BmsI GCATC 2 cut(s) 1050, 1141
BpiI GAAGAC 2 cut(s) 180, 713
BplI GAGNNNNNCTC 2 cut(s) 316, 348
BpmI CTGGAG 1 cut(s) 1000
BpuEI CTTGAG 1 cut(s) 551
BsaAI YACGTR 1 cut(s) 867
BsaJI CCNNGG 2 cut(s) 508, 558
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bse1I ACTGG 1 cut(s) 744
Bse3DI GCAATG 2 cut(s) 671, 992
BseBI CCWGG 1 cut(s) 560
BseDI CCNNGG 2 cut(s) 508, 558
BseGI GGATG 1 cut(s) 667
BseMI GCAATG 2 cut(s) 671, 992
BseMII CTCAG 2 cut(s) 597, 990
BseNI ACTGG 1 cut(s) 744
BseXI GCAGC 3 cut(s) 40, 94, 955
BsgI GTGCAG 2 cut(s) 284, 396
Bsh1236I CGCG 1 cut(s) 1056
BshFI GGCC 1 cut(s) 536
BsmAI GTCTC 6 cut(s) 6, 19, 273, 678, 926, 1104
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 558
Bsp19I CCATGG 1 cut(s) 508
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 2 cut(s) 598, 991
BspFNI CGCG 1 cut(s) 1056
BspLI GGNNCC 1 cut(s) 665
BspMI ACCTGC 1 cut(s) 1016
BspQI GCTCTTC 1 cut(s) 372
BsrDI GCAATG 2 cut(s) 671, 992
BsrI ACTGG 1 cut(s) 744
BssECI CCNNGG 2 cut(s) 508, 558
BssT1I CCWWGG 1 cut(s) 508
Bst2UI CCWGG 1 cut(s) 560
Bst6I CTCTTC 4 cut(s) 372, 509, 1014, 1119
BstBAI YACGTR 1 cut(s) 867
BstC8I GCNNGC 2 cut(s) 678, 882
BstDEI CTNAG 4 cut(s) 371, 606, 999, 1015
BstDSI CCRYGG 1 cut(s) 508
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 1 cut(s) 1056
BstH2I RGCGCY 1 cut(s) 440
BstHHI GCGC 3 cut(s) 439, 906, 1056
BstMAI GTCTC 6 cut(s) 6, 19, 273, 678, 926, 1104
BstMWI GCNNNNNNNGC 3 cut(s) 562, 1060, 1160
BstNI CCWGG 1 cut(s) 560
BstNSI RCATGY 2 cut(s) 761, 965
BstSCI CCNGG 1 cut(s) 558
BstSFI CTRYAG 2 cut(s) 411, 706
BstUI CGCG 1 cut(s) 1056
BstV1I GCAGC 3 cut(s) 40, 94, 955
BstV2I GAAGAC 2 cut(s) 180, 713
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 508
BtrI CACGTC 1 cut(s) 731
BtsCI GGATG 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 66, 360
BveI ACCTGC 1 cut(s) 1016
Cac8I GCNNGC 2 cut(s) 678, 882
CaiI CAGNNNCTG 1 cut(s) 565
CfoI GCGC 3 cut(s) 439, 906, 1056
Cfr13I GGNCC 1 cut(s) 427
Csp6I GTAC 2 cut(s) 655, 816
CviAII CATG 6 cut(s) 104, 451, 509, 658, 758, 962
CviQI GTAC 2 cut(s) 655, 816
DdeI CTNAG 4 cut(s) 371, 606, 999, 1015
Eam1104I CTCTTC 4 cut(s) 372, 509, 1014, 1119
EarI CTCTTC 4 cut(s) 372, 509, 1014, 1119
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 558
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 1 cut(s) 427
Eco47III AGCGCT 1 cut(s) 438
Eco57I CTGAAG 2 cut(s) 90, 150
EcoRII CCWGG 1 cut(s) 558
EcoRV GATATC 1 cut(s) 895
EcoT14I CCWWGG 1 cut(s) 508
EcoT22I ATGCAT 2 cut(s) 967, 1165
EcoT38I GRGCYC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 6 cut(s) 107, 454, 512, 661, 761, 965
FalI AAGNNNNNCTT 2 cut(s) 998, 1030
FatI CATG 6 cut(s) 103, 450, 508, 657, 757, 961
FauNDI CATATG 1 cut(s) 1165
Fnu4HI GCNGC 3 cut(s) 54, 108, 944
FokI GGATG 1 cut(s) 654
FriOI GRGCYC 1 cut(s) 558
Fsp4HI GCNGC 3 cut(s) 54, 108, 944
FspBI CTAG 4 cut(s) 399, 440, 681, 1183
GlaI GCGC 3 cut(s) 438, 905, 1055
GluI GCNGC 3 cut(s) 54, 108, 944
GsuI CTGGAG 1 cut(s) 1000
HaeII RGCGCY 1 cut(s) 440
HaeIII GGCC 1 cut(s) 536
HhaI GCGC 3 cut(s) 439, 906, 1056
Hin1II CATG 6 cut(s) 107, 454, 512, 661, 761, 965
Hin6I GCGC 3 cut(s) 437, 904, 1054
HinP1I GCGC 3 cut(s) 437, 904, 1054
HinfI GANTC 1 cut(s) 395
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 2 cut(s) 617, 1042
Hpy188I TCNGA 6 cut(s) 130, 481, 589, 607, 826, 1082
Hpy188III TCNNGA 4 cut(s) 399, 492, 530, 764
Hpy8I GTNNAC 2 cut(s) 617, 1042
Hpy99I CGWCG 1 cut(s) 732
HpyAV CCTTC 5 cut(s) 68, 77, 396, 617, 1085
HpyCH4IV ACGT 2 cut(s) 730, 866
HpyCH4V TGCA 9 cut(s) 265, 295, 377, 450, 523, 831, 965, 1063, 1163
HpyF10VI GCNNNNNNNGC 3 cut(s) 562, 1060, 1160
HpyF3I CTNAG 4 cut(s) 371, 606, 999, 1015
HpySE526I ACGT 2 cut(s) 730, 866
Hsp92II CATG 6 cut(s) 107, 454, 512, 661, 761, 965
HspAI GCGC 3 cut(s) 437, 904, 1054
LguI GCTCTTC 1 cut(s) 372
LmnI GCTCC 2 cut(s) 562, 877
LpnPI CCDG 9 cut(s) 505, 545, 572, 690, 725, 964, 1011, 1011, 1084
Lsp1109I GCAGC 3 cut(s) 40, 94, 955
LweI GCATC 2 cut(s) 1050, 1141
MaeI CTAG 4 cut(s) 399, 440, 681, 1183
MaeII ACGT 2 cut(s) 730, 866
MaeIII GTNAC 1 cut(s) 1129
MhlI GDGCHC 1 cut(s) 558
MluCI AATT 5 cut(s) 543, 851, 859, 887, 1136
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 399
Mph1103I ATGCAT 2 cut(s) 967, 1165
MroXI GAANNNNTTC 2 cut(s) 384, 577
MseI TTAA 3 cut(s) 702, 836, 1005
MslI CAYNNNNRTG 3 cut(s) 513, 729, 826
MspA1I CMGCKG 2 cut(s) 711, 978
MspR9I CCNGG 1 cut(s) 560
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 1 cut(s) 560
MvnI CGCG 1 cut(s) 1056
MwoI GCNNNNNNNGC 3 cut(s) 562, 1060, 1160
NcoI CCATGG 1 cut(s) 508
NdeI CATATG 1 cut(s) 1165
NlaIII CATG 6 cut(s) 107, 454, 512, 661, 761, 965
NlaIV GGNNCC 1 cut(s) 665
NsiI ATGCAT 2 cut(s) 967, 1165
NspI RCATGY 2 cut(s) 761, 965
PciSI GCTCTTC 1 cut(s) 372
PctI GAATGC 1 cut(s) 1165
PdmI GAANNNNTTC 2 cut(s) 384, 577
PflFI GACNNNGTC 1 cut(s) 167
PkrI GCNGC 3 cut(s) 55, 109, 945
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Ppu21I YACGTR 1 cut(s) 867
Psp6I CCWGG 1 cut(s) 558
PspGI CCWGG 1 cut(s) 558
PspN4I GGNNCC 1 cut(s) 665
PspPI GGNCC 1 cut(s) 427
PstNI CAGNNNCTG 1 cut(s) 565
PsyI GACNNNGTC 1 cut(s) 167
PvuII CAGCTG 2 cut(s) 711, 978
RsaI GTAC 2 cut(s) 656, 817
RsaNI GTAC 2 cut(s) 655, 816
RseI CAYNNNNRTG 3 cut(s) 513, 729, 826
SapI GCTCTTC 1 cut(s) 372
SaqAI TTAA 3 cut(s) 702, 836, 1005
SatI GCNGC 3 cut(s) 54, 108, 944
Sau96I GGNCC 1 cut(s) 427
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 1 cut(s) 560
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 2 cut(s) 1050, 1141
SfcI CTRYAG 2 cut(s) 411, 706
SinI GGWCC 1 cut(s) 427
SmiMI CAYNNNNRTG 3 cut(s) 513, 729, 826
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
Sse9I AATT 5 cut(s) 543, 851, 859, 887, 1136
SspI AATATT 1 cut(s) 792
SspMI CTAG 4 cut(s) 399, 440, 681, 1183
StyD4I CCNGG 1 cut(s) 558
StyI CCWWGG 1 cut(s) 508
TaiI ACGT 2 cut(s) 733, 869
TaqI TCGA 3 cut(s) 171, 727, 958
TasI AATT 5 cut(s) 543, 851, 859, 887, 1136
TatI WGTACW 1 cut(s) 654
Tru1I TTAA 3 cut(s) 702, 836, 1005
Tru9I TTAA 3 cut(s) 702, 836, 1005
TscAI CASTG 2 cut(s) 73, 367
TseI GCWGC 3 cut(s) 53, 107, 943
TspDTI ATGAA 3 cut(s) 713, 960, 1137
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 2 cut(s) 73, 367
Tth111I GACNNNGTC 1 cut(s) 167
VpaK11BI GGWCC 1 cut(s) 427
XapI RAATTY 3 cut(s) 851, 887, 1136
XbaI TCTAGA 1 cut(s) 398
XceI RCATGY 2 cut(s) 761, 965
XmnI GAANNNNTTC 2 cut(s) 384, 577
XspI CTAG 4 cut(s) 399, 440, 681, 1183
Zsp2I ATGCAT 2 cut(s) 967, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.