pycom03g09680

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0012214
Physical Location & Seq
Forward (+)
8411249 .. 8414142
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g09680.3

Sequence Viewer

Length: 1179 bp
ATGGGAAAGCGCGATAAGAAACCGAGACACCGGCAAAGGAGAGCTGCCTTCCACTACACTGAGGAAGACGATGGCGACGGTGATTATGAAGGTCACTCCGCTTCCAAGCTCACTGTTTCCGAAGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGCGGCGGAGGAGGAGGTTGCTGATGAATACCCATCAACTGATATGCCCTCAAAGTTTCTTCTTTATCAACAATCTGTACAGTCGCCGAAAGGAGACATAAGCTATTTGCAGAAGTTCTTTTTGATGTACGTGGGTGGGAGGTTACCTCTCCATCTCCAAGAAGATTTCTGTGGCACTGCGCTGCTTAGTGCAGAATGGCTCCGAACTGACCCGAGAAGGACTGCTGTAGGGTTGGATTTGGACCCTGAAGCGCTAAACTGGTGCATGGAGAACAACATAAATAAAGTTGGGTCTGATGGATATTCCAGAATATCCCTCTTTCACGGGAATGTCTTACAACCTCTTGAGGCCAAACTAATTAAGCCTGAGGCTGAGGAGCAGATGAGAAACATTTCACTCAGTGAGAACGGAGATGGTTTGGAGAGTGATGGATTGCAATCTACTCTTCAAGAGAGCTCTTCTTCCTCACATGATGAGAAGTACTTGAGCAATCATCCGTTGCCTGCTAGAGACATTGTGTGTGCTTTTAACTACAGCTGTTGTTGTCTCCATCAACGTGCTGAACTGGTTCTGTATCTCAAGCATGCTCTTGCTGTCTTGTCAAAGAAAGGTGGAATATTTGTAATGGATTTGTATGGCGGTACATCCTCAGAGTGCAAGCTAAGGCTTCAAAGGAGATTTGCCAATTTTACGTATGTTTGGGAGCAAGCTGAATTTGATATCATTGAACGCAAGACGAGAATTAGCCTCCATTTCCATCTCAAAAAACAGCAGAAGAAAATTCGACATGCGTTTTCATACAGCTGGAGGCTGTGGTCGCTGCCGGAGATCAAGGACTGCTTAGAAGAGGCAGGCTTTAAGTCTGTCCATTTTTGGCTGAGGAAGATGCAAGACAGCGGAGAAATCCGAAGAACAGAGGGTTTTGGTGTTGGGCGAGACATAAAATATGAAGAGGTGAAACATTTTCAGCAACATGATTCATGGAATGCATACATCGTAGGTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

45.25

Weight (kDa)

5.27

Isoelectric Point (pI)

67.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012214)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27590
fragaria_vesca FvH4_3g24630 FvH4_3g24630
malus_domestica MD03G1142000.v1.1 MD11G1162600.v1.1
prunus_persica Prupe.6G127300_v2.0.a1 Prupe.6G127300_v2.0.a1
pyrus_communis pycom03g09680
rosa_chinensis RchiOBHm_Chr5g0044201
rosa_laevigata RLG00000034252
rosa_multiflora Rmu_sc0000954.1_g000008 Rmu_sc0019416.1_g000001
rosa_roxburghii Rroxscaffold_1G00036840
rosa_rugosa Rorug05G0211500 Rorug05G0211600.1
rosa_samantha Rh5BG304000 Rh5CG331100 Rh5DG312700
rosa_wichuraiana Rw5G027450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 12
AciI CCGC 6 cut(s) 99, 167, 170, 173, 807, 1065
AcsI RAATTY 2 cut(s) 881, 948
AcuI CTGAAG 1 cut(s) 435
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 4 cut(s) 246, 296, 650, 811
AfeI AGCGCT 1 cut(s) 420
AgsI TTSAA 3 cut(s) 617, 839, 896
AluBI AGCT 8 cut(s) 44, 109, 270, 624, 705, 829, 878, 972
AluI AGCT 8 cut(s) 44, 109, 270, 624, 705, 829, 878, 972
Alw21I GWGCWC 1 cut(s) 626
Alw26I GTCTC 5 cut(s) 19, 255, 672, 719, 1098
Ama87I CYCGRG 1 cut(s) 379
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 3 cut(s) 44, 349, 988
ApoI RAATTY 2 cut(s) 881, 948
Asp700I GAANNNNTTC 2 cut(s) 559, 735
AspLEI GCGC 3 cut(s) 12, 349, 421
AspS9I GGNCC 1 cut(s) 409
AsuHPI GGTGA 2 cut(s) 92, 1135
AvaI CYCGRG 1 cut(s) 379
AvaII GGWCC 1 cut(s) 409
AxyI CCTNAGG 1 cut(s) 534
BanII GRGCYC 1 cut(s) 626
BbsI GAAGAC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 626
BbvCI CCTCAGC 2 cut(s) 540, 1046
BbvI GCAGC 3 cut(s) 31, 336, 975
BccI CCATC 8 cut(s) 65, 208, 327, 458, 575, 590, 726, 933
BcoDI GTCTC 5 cut(s) 19, 255, 672, 719, 1098
BfaI CTAG 1 cut(s) 675
BfmI CTRYAG 2 cut(s) 393, 700
BfoI RGCGCY 1 cut(s) 422
BisI GCNGC 5 cut(s) 45, 168, 171, 350, 989
BlsI GCNGC 5 cut(s) 46, 169, 172, 351, 990
BmcAI AGTACT 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 409
BmeT110I CYCGRG 1 cut(s) 379
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 2 cut(s) 368, 411
BmsI GCATC 1 cut(s) 1044
BpiI GAAGAC 1 cut(s) 72
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpmI CTGGAG 1 cut(s) 994
Bpu10I CCTNAGC 3 cut(s) 540, 830, 1046
BpuEI CTTGAG 3 cut(s) 533, 673, 731
BsaAI YACGTR 2 cut(s) 298, 861
BsaBI GATNNNNATC 1 cut(s) 604
Bse118I RCCGGY 1 cut(s) 30
Bse1I ACTGG 2 cut(s) 431, 738
Bse21I CCTNAGG 1 cut(s) 534
Bse8I GATNNNNATC 1 cut(s) 604
BseGI GGATG 2 cut(s) 661, 812
BseJI GATNNNNATC 1 cut(s) 604
BseMII CTCAG 6 cut(s) 51, 525, 531, 580, 831, 1037
BseNI ACTGG 2 cut(s) 431, 738
BseXI GCAGC 3 cut(s) 31, 336, 975
BsgI GTGCAG 1 cut(s) 378
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 1 cut(s) 518
BsiHKAI GWGCWC 1 cut(s) 626
BsiHKCI CYCGRG 1 cut(s) 379
BsiSI CCGG 2 cut(s) 31, 992
BsmAI GTCTC 5 cut(s) 19, 255, 672, 719, 1098
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 1 cut(s) 518
BsoBI CYCGRG 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 626
Bsp1407I TGTACA 1 cut(s) 244
Bsp143I GATC 1 cut(s) 996
BspACI CCGC 6 cut(s) 99, 167, 170, 173, 807, 1065
BspANI GGCC 1 cut(s) 518
BspCNI CTCAG 6 cut(s) 52, 526, 532, 579, 830, 1038
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 2 cut(s) 368, 411
BspQI GCTCTTC 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 30
BsrGI TGTACA 1 cut(s) 244
BsrI ACTGG 2 cut(s) 431, 738
BssAI RCCGGY 1 cut(s) 30
BssMI GATC 1 cut(s) 996
Bst4CI ACNGT 3 cut(s) 80, 115, 249
Bst6I CTCTTC 5 cut(s) 123, 618, 631, 1008, 1113
BstAUI TGTACA 1 cut(s) 244
BstBAI YACGTR 2 cut(s) 298, 861
BstC8I GCNNGC 5 cut(s) 672, 753, 827, 876, 1021
BstDEI CTNAG 9 cut(s) 60, 353, 534, 540, 566, 817, 830, 1009, 1046
BstEII GGTNACC 1 cut(s) 309
BstF5I GGATG 2 cut(s) 661, 812
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 3 cut(s) 12, 349, 421
BstKTI GATC 1 cut(s) 999
BstMAI GTCTC 5 cut(s) 19, 255, 672, 719, 1098
BstMBI GATC 1 cut(s) 996
BstMWI GCNNNNNNNGC 1 cut(s) 985
BstNSI RCATGY 2 cut(s) 755, 959
BstPI GGTNACC 1 cut(s) 309
BstSFI CTRYAG 2 cut(s) 393, 700
BstSNI TACGTA 1 cut(s) 861
BstUI CGCG 1 cut(s) 12
BstV1I GCAGC 3 cut(s) 31, 336, 975
BstV2I GAAGAC 1 cut(s) 72
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 1 cut(s) 518
BtsCI GGATG 2 cut(s) 661, 812
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 4 cut(s) 57, 111, 342, 574
Cac8I GCNNGC 5 cut(s) 672, 753, 827, 876, 1021
CfoI GCGC 3 cut(s) 12, 349, 421
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 409
Csp6I GTAC 4 cut(s) 245, 295, 649, 810
CviAII CATG 6 cut(s) 433, 638, 752, 956, 1142, 1149
CviQI GTAC 4 cut(s) 245, 295, 649, 810
DdeI CTNAG 9 cut(s) 60, 353, 534, 540, 566, 817, 830, 1009, 1046
DpnI GATC 1 cut(s) 998
DpnII GATC 1 cut(s) 996
DraIII CACNNNGTG 1 cut(s) 569
Eam1104I CTCTTC 5 cut(s) 123, 618, 631, 1008, 1113
EarI CTCTTC 5 cut(s) 123, 618, 631, 1008, 1113
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 624
Eco105I TACGTA 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 626
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 409
Eco47III AGCGCT 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 624
Eco57I CTGAAG 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 379
Eco91I GGTNACC 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 624
EcoO65I GGTNACC 1 cut(s) 309
EcoRV GATATC 1 cut(s) 889
EcoT22I ATGCAT 1 cut(s) 1159
EcoT38I GRGCYC 1 cut(s) 626
FaeI CATG 6 cut(s) 436, 641, 755, 959, 1145, 1152
FalI AAGNNNNNCTT 2 cut(s) 992, 1024
FatI CATG 6 cut(s) 432, 637, 751, 955, 1141, 1148
Fnu4HI GCNGC 5 cut(s) 45, 168, 171, 350, 989
FokI GGATG 2 cut(s) 648, 799
FriOI GRGCYC 1 cut(s) 626
Fsp4HI GCNGC 5 cut(s) 45, 168, 171, 350, 989
FspBI CTAG 1 cut(s) 675
GlaI GCGC 3 cut(s) 11, 348, 420
GluI GCNGC 5 cut(s) 45, 168, 171, 350, 989
GsuI CTGGAG 1 cut(s) 994
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 1 cut(s) 518
HapII CCGG 2 cut(s) 31, 992
HhaI GCGC 3 cut(s) 12, 349, 421
Hin1II CATG 6 cut(s) 436, 641, 755, 959, 1145, 1152
Hin6I GCGC 3 cut(s) 10, 347, 419
HinP1I GCGC 3 cut(s) 10, 347, 419
HinfI GANTC 1 cut(s) 1145
HpaII CCGG 2 cut(s) 31, 992
HphI GGTGA 2 cut(s) 92, 1135
Hpy188I TCNGA 5 cut(s) 121, 371, 463, 820, 1076
Hpy188III TCNNGA 3 cut(s) 474, 512, 617
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 58, 83, 378
HpyCH4III ACNGT 3 cut(s) 80, 115, 249
HpyCH4IV ACGT 3 cut(s) 297, 724, 860
HpyCH4V TGCA 8 cut(s) 277, 359, 432, 604, 825, 1057, 1157, 1175
HpyF10VI GCNNNNNNNGC 1 cut(s) 985
HpyF3I CTNAG 9 cut(s) 60, 353, 534, 540, 566, 817, 830, 1009, 1046
HpySE526I ACGT 3 cut(s) 297, 724, 860
Hsp92II CATG 6 cut(s) 436, 641, 755, 959, 1145, 1152
HspAI GCGC 3 cut(s) 10, 347, 419
Kzo9I GATC 1 cut(s) 996
LguI GCTCTTC 1 cut(s) 631
LmnI GCTCC 3 cut(s) 372, 544, 871
Lsp1109I GCAGC 3 cut(s) 31, 336, 975
LweI GCATC 1 cut(s) 1044
MaeI CTAG 1 cut(s) 675
MaeII ACGT 3 cut(s) 297, 724, 860
MaeIII GTNAC 2 cut(s) 92, 309
MalI GATC 1 cut(s) 998
MboI GATC 1 cut(s) 996
MhlI GDGCHC 1 cut(s) 626
MluCI AATT 5 cut(s) 525, 853, 881, 909, 948
MmeI TCCRAC 1 cut(s) 381
Mph1103I ATGCAT 1 cut(s) 1159
MroXI GAANNNNTTC 2 cut(s) 559, 735
MseI TTAA 3 cut(s) 528, 696, 1026
MslI CAYNNNNRTG 2 cut(s) 495, 723
MspA1I CMGCKG 3 cut(s) 705, 972, 1065
MspI CCGG 2 cut(s) 31, 992
Mva1269I GAATGC 1 cut(s) 1159
MvnI CGCG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 985
NdeII GATC 1 cut(s) 996
NlaIII CATG 6 cut(s) 436, 641, 755, 959, 1145, 1152
NlaIV GGNNCC 2 cut(s) 368, 411
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 1 cut(s) 1159
NspI RCATGY 2 cut(s) 755, 959
PaeI GCATGC 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 631
PctI GAATGC 1 cut(s) 1159
PdmI GAANNNNTTC 2 cut(s) 559, 735
PfeI GAWTC 1 cut(s) 1145
PkrI GCNGC 5 cut(s) 46, 169, 172, 351, 990
Ppu21I YACGTR 2 cut(s) 298, 861
Psp124BI GAGCTC 1 cut(s) 626
PspEI GGTNACC 1 cut(s) 309
PspN4I GGNNCC 2 cut(s) 368, 411
PspPI GGNCC 1 cut(s) 409
PvuII CAGCTG 2 cut(s) 705, 972
RsaI GTAC 4 cut(s) 246, 296, 650, 811
RsaNI GTAC 4 cut(s) 245, 295, 649, 810
RseI CAYNNNNRTG 2 cut(s) 495, 723
SacI GAGCTC 1 cut(s) 626
SapI GCTCTTC 1 cut(s) 631
SaqAI TTAA 3 cut(s) 528, 696, 1026
SatI GCNGC 5 cut(s) 45, 168, 171, 350, 989
Sau3AI GATC 1 cut(s) 996
Sau96I GGNCC 1 cut(s) 409
ScaI AGTACT 1 cut(s) 650
SduI GDGCHC 1 cut(s) 626
SfaNI GCATC 1 cut(s) 1044
SfcI CTRYAG 2 cut(s) 393, 700
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 2 cut(s) 495, 723
SmlI CTYRAG 3 cut(s) 512, 652, 746
SmoI CTYRAG 3 cut(s) 512, 652, 746
SnaBI TACGTA 1 cut(s) 861
SphI GCATGC 1 cut(s) 755
Sse9I AATT 5 cut(s) 525, 853, 881, 909, 948
SsiI CCGC 6 cut(s) 99, 167, 170, 173, 807, 1065
SspI AATATT 1 cut(s) 786
SspMI CTAG 1 cut(s) 675
SstI GAGCTC 1 cut(s) 626
TaaI ACNGT 3 cut(s) 80, 115, 249
TaiI ACGT 3 cut(s) 300, 727, 863
TaqI TCGA 1 cut(s) 952
TasI AATT 5 cut(s) 525, 853, 881, 909, 948
TatI WGTACW 2 cut(s) 244, 648
TauI GCSGC 2 cut(s) 170, 173
TfiI GAWTC 1 cut(s) 1145
Tru1I TTAA 3 cut(s) 528, 696, 1026
Tru9I TTAA 3 cut(s) 528, 696, 1026
TscAI CASTG 4 cut(s) 64, 118, 349, 574
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 3 cut(s) 44, 349, 988
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 5 cut(s) 102, 207, 954, 1131, 1137
TspGWI ACGGA 2 cut(s) 591, 654
TspRI CASTG 4 cut(s) 64, 118, 349, 574
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 2 cut(s) 881, 948
XceI RCATGY 2 cut(s) 755, 959
XmnI GAANNNNTTC 2 cut(s) 559, 735
XspI CTAG 1 cut(s) 675
ZrmI AGTACT 1 cut(s) 650
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.