Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003576

Physical Location & Seq

Genomic Coordinates Forward (+)

23026054 .. 23036041

Gene Structure 9988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1169900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4974 bp

ATGGAGTTCGTGGGAAGAAGTGTGAGGAAAGAGTTCAAAGGCCATGGTTTTTTTGCCGGAACTGTGAAATCGTACGCCGCCGCATCTGGGTTGTTCGAGGTCGTCTATGAAAATGGAAATACTGAGGAATTGAGCTTCGATGAGGTCTCTCTTCTCCTTGGTGGTGGTGCTGAGGCTGACCTCGTGGAGGTCGCCGCCAAGCGGAGCCGCCTCGGCCGGAAGCCCAAAAAACGGCGCCGTATTGAGAGGAAGCGAGAAATTCGAGGTAATGCTGCTCTTGGAAATGATTCTGGGTTTGGTGGGGATTTGAATGAAAATTGTAATTTGAGTGATGGGTCTGAGGGAAAATTGGAAGTTGATCAGGGGTTTGGAGGGAATTTGAGGAAAAATGTTGCGGTTAGTGGGATTGTGAATGAAAATGTTGATTCTAGAAATGTAGTGGATAAGACCCTAGAGCAGGAAACTGGGTTGATTGCTAATGGGGATGTGAATGAAGTCGATAATTTGAAAAATGGGATTGATTTGAATGCTGGGTTTAATTTGAATTTGAATGATGGGTGTGACTTGAATGTTGATCCAAATGTTGGTAAGGAAGAAATTGCAGAGATAAGAGATTGTATTGATTTGAATTTGGATGCGAATGATGAGTTTGTTGAGAATCAGAATGTGGATAGTTTGGGCGGTTCAGCTGTGCTCACCCACGGAAGCCAGAGGAGGGGATGCAATTTCGATCTGAATTTGGAAGTTAATGAAGAATTTAAGGATACAGAAGGTGACTGTGAAGAAAAATTCAAGGTTAATCCCAGGTTTGAATTGGTTGAAGAAAGTCTAAAGAAAGAGAGGAGTGGAGATGCTGAAGAAAAGGTTATTGACGATGGTAATTCTAATGAGACCTGGAAGGAAGTGTATATTGATATTAATGAAGATATTCCCATGACGAGCGTTGATGACCCCATTGACTGTGCAGCTGGTGAACGGCTTGATAATACATATTGTTGTTCCAGTGGAGATCTGAAGGCTGATGGTTCTCTTGGGATTTTTGAGACTAGCTGCATTAATGATAGTGGATTGGTGGAAGTTCCGGTGAAGGATAGTCTCTATGAGGCCCGCACTCCAATGATTCATGGAAACCTAGGCGATTCAGGAAGTCCATGCATTCAAAAAAGTAGCCGTAGAAAGAGAAGAAAACTACTGGATAATTTGATATCTACGACTACAGAGACAGTTTTGAGGAGAAGCGCTCGTAGAGGTTCTGCTCAAAATCATGTTTCTGTCAGCGATCCATTTTCCTCTTCTGCAGTGAGTGCAATAACAGAGGAAAAACCATCGATTTCTGGTTGCGAAGAGGCTGAAAATCCTAGTGTTCTTTTTCAGGAGCTGGAATTACCTCCTTCATCACAACATTTAAATCTAGATGGAATTCCCATACTTGACGTTTTTTCTATTTATGCTTGTTTAAGATCATTTAGCACTCTATTATTTCTGAGTCCCTTCAAGTTGGAGGATTTTGTGGCCGCACTGACGTGCAAGTCTCCTAGCTCATTATTTGATCATGTTCATGTTTCTATTTTGCAAACACTGAGAAAACACTTGGAGTGGCTTGCAAATGATGGTTCCGAAACAGCTTCTGATTGTCTGAGGAGTCTTAATTGGGATTTTCTTGACTTGATTACATGGCCGATTTTTATGGTTGAGTATTTCTTGATCCATAGTTCGGGACTGAAGCCAGGTTTTGATCTCAGTTGCTTCAAGTTATACAAAACTGACTACTACACACAACCTGCTTCTGTAAAAGTTGAGATACTGCGGTGTCTATGTGATGATTTGATAGAAGTGGAAGCCATGAGGTTAGAAATTAATAGAAGGTCTTTGGCTGCTGAGCCTGACATGGTCAATGACCGGAATTTGAACTATGAGGTTTGTAAGAAAAGGAAGGCTCTAGTGGAGGTTGCTGGTACCTGTAATGCGAATGATGAGGTTGATGATGACACCACCGACTGGAATAGTGATGAATGCTGCCTCTGTAAGATGGATGGAAATTTAATCTGCTGTGATGGCTGTCCTGCTGCATATCATTCAAAATGTGTTGGAGTTTCTAATGATCTTTTGCCGGAGGGTGACTGGTACTGCCCTGAGTGTTTGATTGACAGGCATAGACCTTGGATGAAATTGCAGAAGTCACTCCGAGGTGCAGAGTTATTAGGAATTGATCCTCGTGGTCGGCTATATTTTAAAAGTTTTGGGTACCTTTTGGTATCAGATTCTTTTGATAGCGAGTCCGCATTCAACTATTACACCAGAGATGATCTGAATAAAGTTATTAAAGTGCTAAGGTCTTCAGGTTTTTTCTATGGTGGGATATTAGTGGCCATCTGCAAGCACTGGGACATTCCTGTTAGCTTTGATGGAGCATATAGTGAAATTGGCTGCTTGATGCCCCCGTACCCATTGGCATTTTCAGAAACATGTGCTGTTAAGAATGAGACTGATGAAGATAGAAAATTGCAAGAGAATTCAGGGACTGTGACAGCAATTCCAAATATAGCTTCTGAAGTGTCAGCTGAAACCATGCAAGTGGAAAGGTCAGTCATGCTGTGCGATCCAGATGAACCTGACTTTGTGGGACTTCATCCTGAAGACTGTTCTTTACCATCTGCTAGCTTGCATGTCAGAAAAGGAATCACTAGAAAAGGGGGAACTTCAGAAATGCATTGTGGAATTGGTTATATGAACTATTACAGTTTTGGCCAAATTGCTTCATCTGTTGCTGAGGAGTTGACGCGTAAACCATCAGAAAAAATTAAAGAAGATAAAATAATAACAGAAGAGGAAATAGTATCAGCACAGATGAAGACCATTTTAAAAAAATCTTCCAAGTTTTTCTGGCCAAATATTGAGGACCTAAATACTGGTGCACGGAAAGAGAAATGCGGTTGGTGCTTTTCTTGCAAAGCTCCTGCTGATGACAAGGATTGCTTGTTTATCATGAGTATGGGGTCTATCAAGGATGCTTCTAATAGTGATAGAGTTGGCCTTCAATCCAAAAAGAATGGGAAAGGTCATCTTAATGATGTCAGCTGTCAAATCCTATCTATTCATGATCGTCTGCAGGGACTTCTGTTGGGCCCATGGTTGAGTCCACTTCACACAGAACTCTGGCGTAAATACCTTCTTTATGCATCTGGCATTGCCTCCATTAAATATTTACTTCTCATGTTGGAGGCAAATTTGCGTCATCGTGCACTTTCAGCGGATTGGTTGAAACATGTAGATTCTGTTAATACCATGGGTTCAGCTTCTCATGTTGTGACTTCATTACGTAGCAGAAGAAGGCCCAAGTGTTCAGATAGTGAGTCCAACCCCCCTTTAAATGCTGCCAGTGGATTGGGAATGTTTTGGTGGAGGGGTGGTAGGCTTTCTCGTCAGGTTTTCAGTTGGAAGGTTTTGCCTCGTTCCTTGACTTCCAAGGCTGCCAGACAAGCTGGGTGTACAAAGATACTGGGTATATTATATCCTGAGAATTCAGAATATGCTAAGAGAAGCAAATCTGTTGCCTGGCGAGCTGCTGTTGAGGCCTCAACTAGTGCAGAACAGCTTGCTTTACAGGTTAGAGAGCTTGATTCAAACATTAGATGGGATGATATTGAGAATTCACATCCTCTGCCAACATTGGACAAAGAATCTAGAAAATCAATCAAGCTGTTCAAGAAAGTTATTGTCCGCAGGAAGTCCTCTGAAGGGGGAGCAGTTAAGTATCTTCTTGATTTTGGTAAGAGAAGAGCTATCCCTGACATTGTGAAGGGATTTGGTTCTTTGGTTGAAGAACTGTCTAGTGAGAAGAAAAAGTATTGGCTGGATGAATCATATCTTCCCTTGCATCTTTTGAAAAGTTTTGAGGAGAAAAGGATTGCCCGCAAGTCAACTGATGTGAAATCTGGTAAAGTTCTTGAGGTTGCTAGAGTAACAAAGAGGCCCCGGGAAGAAAAGGGATTTATGTATTTGTTTTCGAAGGCTGAAAGATCTGAGTATTATAAATGTGCACACTGCAACAAAGATGTCCTGATAAGGGAAGCTGTGAGTTGCCAGTCTTGCGAAGGATTTTTTCACAAAAGGCATGCCAGGAAGTCTGCTGGGGCAGTGGTGGCTCGGTATAAATATACATGCTACCGATGCCAGAAGGGATTGCGTCCGAAGATTGACACAAAAAGAAGGAAAGTTGAAACAAAAGGAGGGAAAGTACAGCCACATAAATGTAAACCGAAAGGAGGGAAAGTACAGTCACAAAAGTTTACAAATTCTCAAACAGGTCGCAGATCGATGCGTTTGAAAAACAAAAAAAAAGCTTTAGCTGGAGGACGACAAGTAAGATTGAAAAATAGCAAAACAGTTCCAGCAAGTGTACCCCTACGTCGTTCTCCTAGAAAAGCTAAATGTTTACCGGTGCAAAATAAAAGGCATAGCAAACGCAAGAAGGGGAAAAAAGGCAAATCCAATAAGGGCACATATAAGAAACCAAATACAGCTACATGGCAGAAGAAGAGAACGCAAGTTTATCACAGTTACTGGCTTAATGGTCTGCTGTTGTCCAGAAAGCCAAGTGATGAACGTGTTATGCATTTTAGGGATAAGAAACTCCTTGTGCATTCAGAATGTGTGTCAACCATTCTTGATCAACTCAAATGCCACCTTTGTTGTGAAGCAAGATATTCGTCTTCTTTAAATTATATTTCTTGTGAAATATGTGGAGTGTGGTTTCACGGTGATGCTTTTGGACTGAATTCGGAGAACATTGGTAAGCTGATTGGATTTAAGTGCCACGTGTGTCGTGAAGGGAACCCTCCTATCTGCCCCCACTTGGAAGATGTGAAAACTGATGTCCCTCAATTGGCTGAAGCTCAAATTGATGGTACCGTTGATTGTTCTGAGGAAGTACCTAACTCTGTCCCACCTTTAAGTGAGATCACATGCAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031010 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DDT PHD WHIM1

Protein Analysis

1658

Amino Acids

185.3

Weight (kDa)

8.12

Isoelectric Point (pI)

47.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PTM_DIR17_Tudor	PF21743	5 - 50	1e-12	PTM/DIR17-like, Tudor domain
DDT	PF02791	477 - 530	3.8e-16	DDT domain
PHD	PF00628	672 - 713	2e-07	PHD-finger
Chromo_PTM	PF24294	1233 - 1305	2.6e-24	Membrane-bound transcription factor PTM, chromo domain-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0003576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12400
fragaria_vesca	FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290 FvH4_3g28290
malus_domestica	MD03G1169900.v1.1 MD11G1187100.v1.1
prunus_persica	Prupe.4G257200_v2.0.a1 Prupe.4G257200_v2.0.a1 Prupe.4G257200_v2.0.a1 Prupe.4G257200_v2.0.a1 Prupe.4G257200_v2.0.a1
pyrus_communis	pycom03g12620 pycom11g16390 pycom11g16400
rosa_chinensis	RchiOBHm_Chr5g0051171
rosa_laevigata	RLG00000034759
rosa_multiflora	Rmu_co8421621.1_g000001 Rmu_sc0000446.1_g000011 Rmu_sc0020509.1_g000001
rosa_roxburghii	Rroxscaffold_1G00029270 Rroxscaffold_1G00029280
rosa_rugosa	Rorug05G0266200
rosa_samantha	Rh5AG338600 Rh5BG348500 Rh5CG375600 Rh5DG361400
rosa_wichuraiana	Rw0G023670 Rw5G031980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4032
AasI	GACNNNNNNGTC	1 cut(s)	1528
Acc36I	ACCTGC	1 cut(s)	1789
Acc65I	GGTACC	3 cut(s)	1955, 2244, 4907
AccB1I	GGYRCC	4 cut(s)	234, 1955, 2244, 4907
AccB7I	CCANNNNNTGG	2 cut(s)	584, 1998
AccII	CGCG	1 cut(s)	2782
AclWI	GGATC	5 cut(s)	569, 1274, 1699, 2204, 2594
AcoI	YGGCCR	6 cut(s)	214, 1512, 1676, 2367, 2746, 2885
AcuI	CTGAAG	9 cut(s)	876, 1034, 1742, 2322, 2571, 2655, 2685, 3756, 4911
AcvI	CACGTG	1 cut(s)	4819
AcyI	GRCGYC	1 cut(s)	235
AfeI	AGCGCT	1 cut(s)	1240
AflIII	ACRYGT	5 cut(s)	2465, 2780, 3265, 4606, 4818
AgeI	ACCGGT	1 cut(s)	4438
AhlI	ACTAGT	1 cut(s)	3581
AjiI	CACGTC	1 cut(s)	1524
AjnI	CCWGG	5 cut(s)	803, 893, 1726, 3554, 4118
AjuI	GAANNNNNNNTTGG	2 cut(s)	2530, 2562
AleI	CACNNNNGTG	1 cut(s)	1522
Alw21I	GWGCWC	4 cut(s)	696, 2917, 3244, 4042
Alw26I	GTCTC	7 cut(s)	151, 884, 1037, 1100, 1214, 1536, 2477
Alw44I	GTGCAC	3 cut(s)	2913, 3240, 4038
AlwI	GGATC	5 cut(s)	569, 1274, 1699, 2204, 2594
AlwNI	CAGNNNCTG	4 cut(s)	1378, 1628, 2522, 4563
Ama87I	CYCGRG	1 cut(s)	3975
Aor51HI	AGCGCT	1 cut(s)	1240
ApaI	GGGCCC	1 cut(s)	3128
ApaLI	GTGCAC	3 cut(s)	2913, 3240, 4038
AseI	ATTAAT	3 cut(s)	918, 1056, 1857
AsiGI	ACCGGT	1 cut(s)	4438
Asp700I	GAANNNNTTC	3 cut(s)	32, 286, 927
Asp718I	GGTACC	3 cut(s)	1955, 2244, 4907
AspA2I	CCTAGG	1 cut(s)	1132
AspLEI	GCGC	2 cut(s)	237, 1241
AspS9I	GGNCC	6 cut(s)	1105, 2899, 3124, 3125, 3333, 3972
AsuC2I	CCSGG	2 cut(s)	3976, 3977
AsuHPI	GGTGA	6 cut(s)	688, 785, 983, 1096, 2129, 4772
AsuII	TTCGAA	1 cut(s)	4007
AsuNHI	GCTAGC	1 cut(s)	2657
AvaI	CYCGRG	1 cut(s)	3975
AvaII	GGWCC	1 cut(s)	2899
AvrII	CCTAGG	1 cut(s)	1132
BaeGI	GKGCMC	5 cut(s)	2917, 3128, 3244, 4042, 4502
BaeI	ACNNNNGTAYC	2 cut(s)	1793, 1826
BalI	TGGCCA	3 cut(s)	2369, 2748, 2887
BanI	GGYRCC	4 cut(s)	234, 1955, 2244, 4907
BanII	GRGCYC	1 cut(s)	3128
BauI	CACGAG	2 cut(s)	182, 2214
BbrPI	CACGTG	1 cut(s)	4819
BbsI	GAAGAC	4 cut(s)	2328, 2643, 2858, 4704
Bbv12I	GWGCWC	4 cut(s)	696, 2917, 3244, 4042
BbvCI	CCTCAGC	2 cut(s)	171, 2769
BceAI	ACGGC	4 cut(s)	222, 248, 992, 1155
BcgI	CGANNNNNNTGC	2 cut(s)	4689, 4723
BciT130I	CCWGG	5 cut(s)	805, 895, 1728, 3556, 4120
BciVI	GTATCC	1 cut(s)	757
BclI	TGATCA	3 cut(s)	358, 1549, 4669
BcnI	CCSGG	2 cut(s)	3976, 3977
BcoDI	GTCTC	7 cut(s)	151, 884, 1037, 1100, 1214, 1536, 2477
BcuI	ACTAGT	1 cut(s)	3581
BfmI	CTRYAG	3 cut(s)	1215, 1296, 3107
BfoI	RGCGCY	2 cut(s)	238, 1242
BfuAI	ACCTGC	1 cut(s)	1789
BfuI	GTATCC	1 cut(s)	757
BglI	GCCNNNNNGGC	1 cut(s)	213
BglII	AGATCT	2 cut(s)	1009, 4019
BlnI	CCTAGG	1 cut(s)	1132
BlpI	GCTNAGC	1 cut(s)	1878
Bme1390I	CCNGG	7 cut(s)	805, 895, 1728, 3556, 3976, 3977, 4120
Bme18I	GGWCC	1 cut(s)	2899
BmeT110I	CYCGRG	1 cut(s)	3975
BmgBI	CACGTC	1 cut(s)	1524
BmgT120I	GGNCC	6 cut(s)	1105, 2899, 3124, 3125, 3333, 3972
BmiI	GGNNCC	9 cut(s)	206, 236, 1615, 1957, 2246, 3126, 3974, 4835, 4909
BmrFI	CCNGG	7 cut(s)	805, 895, 1728, 3556, 3976, 3977, 4120
BmrI	ACTGGG	3 cut(s)	474, 2392, 3509
BmtI	GCTAGC	1 cut(s)	2661
BmuI	ACTGGG	3 cut(s)	474, 2392, 3509
BpiI	GAAGAC	4 cut(s)	2328, 2643, 2858, 4704
BplI	GAGNNNNNCTC	2 cut(s)	1225, 1257
BpmI	CTGGAG	1 cut(s)	4371
Bpu10I	CCTNAGC	3 cut(s)	171, 2330, 2769
Bpu1102I	GCTNAGC	1 cut(s)	1878
Bpu14I	TTCGAA	1 cut(s)	4007
BpuEI	CTTGAG	1 cut(s)	3968
BpuMI	CCSGG	2 cut(s)	3976, 3977
Bsa29I	ATCGAT	2 cut(s)	1328, 4316
BsaAI	YACGTR	2 cut(s)	3320, 4819
BsaHI	GRCGYC	1 cut(s)	235
BsaI	GGTCTC	2 cut(s)	151, 884
BsaWI	WCCGGW	3 cut(s)	1081, 1899, 4438
BsaXI	ACNNNNNCTCC	6 cut(s)	3394, 3424, 4221, 4251, 4257, 4287
Bse118I	RCCGGY	1 cut(s)	4438
Bse3DI	GCAATG	1 cut(s)	3186
BseBI	CCWGG	5 cut(s)	805, 895, 1728, 3556, 4120
BseCI	ATCGAT	2 cut(s)	1328, 4316
BseMI	GCAATG	1 cut(s)	3186
BseRI	GAGGAG	6 cut(s)	727, 856, 1246, 1654, 2786, 3911
BseSI	GKGCMC	5 cut(s)	2917, 3128, 3244, 4042, 4502
BseX3I	CGGCCG	1 cut(s)	214
BseYI	CCCAGC	3 cut(s)	530, 3482, 4130
BsgI	GTGCAG	3 cut(s)	984, 2211, 3606
Bsh1236I	CGCG	1 cut(s)	2782
Bsh1285I	CGRYCG	1 cut(s)	217
BshNI	GGYRCC	4 cut(s)	234, 1955, 2244, 4907
BshTI	ACCGGT	1 cut(s)	4438
BshVI	ATCGAT	2 cut(s)	1328, 4316
BsiEI	CGRYCG	1 cut(s)	217
BsiHKAI	GWGCWC	4 cut(s)	696, 2917, 3244, 4042
BsiHKCI	CYCGRG	1 cut(s)	3975
BsiSI	CCGG	7 cut(s)	57, 217, 1082, 1900, 2111, 3976, 4439
BsiWI	CGTACG	1 cut(s)	72
BslFI	GGGAC	8 cut(s)	1473, 1731, 2399, 2533, 2637, 3126, 4862, 4928
BsmAI	GTCTC	7 cut(s)	151, 884, 1037, 1100, 1214, 1536, 2477
BsmFI	GGGAC	8 cut(s)	1473, 1731, 2399, 2533, 2637, 3126, 4862, 4928
BsmI	GAATGC	5 cut(s)	532, 1155, 2018, 2282, 4642
Bso31I	GGTCTC	2 cut(s)	151, 884
BsoBI	CYCGRG	1 cut(s)	3975
Bsp119I	TTCGAA	1 cut(s)	4007
Bsp120I	GGGCCC	1 cut(s)	3124
Bsp1286I	GDGCHC	6 cut(s)	696, 2917, 3128, 3244, 4042, 4502
Bsp1407I	TGTACA	1 cut(s)	3488
Bsp1720I	GCTNAGC	1 cut(s)	1878
Bsp19I	CCATGG	3 cut(s)	43, 3128, 3285
BspDI	ATCGAT	2 cut(s)	1328, 4316
BspFNI	CGCG	1 cut(s)	2782
BspHI	TCATGA	2 cut(s)	2985, 3097
BspLI	GGNNCC	9 cut(s)	206, 236, 1615, 1957, 2246, 3126, 3974, 4835, 4909
BspMAI	CTGCAG	2 cut(s)	1300, 3111
BspMI	ACCTGC	1 cut(s)	1789
BspOI	GCTAGC	1 cut(s)	2661
BspPI	GGATC	5 cut(s)	569, 1274, 1699, 2204, 2594
BspQI	GCTCTTC	1 cut(s)	3772
BspT104I	TTCGAA	1 cut(s)	4007
BspT107I	GGYRCC	4 cut(s)	234, 1955, 2244, 4907
BspTNI	GGTCTC	2 cut(s)	151, 884
BsrDI	GCAATG	1 cut(s)	3186
BsrFI	RCCGGY	1 cut(s)	4438
BsrGI	TGTACA	1 cut(s)	3488
BssAI	RCCGGY	1 cut(s)	4438
BssNI	GRCGYC	1 cut(s)	235
BssSI	CACGAG	2 cut(s)	182, 2214
BssT1I	CCWWGG	7 cut(s)	43, 157, 1132, 2159, 3128, 3285, 3465
Bst2BI	CACGAG	2 cut(s)	182, 2214
Bst2UI	CCWGG	5 cut(s)	805, 895, 1728, 3556, 4120
Bst6I	CTCTTC	6 cut(s)	156, 1297, 1338, 2820, 3772, 4532
BstACI	GRCGYC	1 cut(s)	235
BstAPI	GCANNNNNTGC	1 cut(s)	1304
BstAUI	TGTACA	1 cut(s)	3488
BstBAI	YACGTR	2 cut(s)	3320, 4819
BstBI	TTCGAA	1 cut(s)	4007
BstC8I	GCNNGC	9 cut(s)	1108, 1602, 2378, 2659, 2663, 3561, 3597, 3913, 4116
BstDSI	CCRYGG	4 cut(s)	43, 700, 3128, 3285
BstENI	CCTNNNNNAGG	2 cut(s)	185, 455
BstFNI	CGCG	1 cut(s)	2782
BstH2I	RGCGCY	2 cut(s)	238, 1242
BstHHI	GCGC	2 cut(s)	237, 1241
BstMAI	GTCTC	7 cut(s)	151, 884, 1037, 1100, 1214, 1536, 2477
BstMCI	CGRYCG	1 cut(s)	217
BstNI	CCWGG	5 cut(s)	805, 895, 1728, 3556, 4120
BstNSI	RCATGY	6 cut(s)	2469, 2669, 3269, 4118, 4164, 4968
BstSCI	CCNGG	7 cut(s)	803, 893, 1726, 3554, 3974, 3975, 4118
BstSFI	CTRYAG	3 cut(s)	1215, 1296, 3107
BstSLI	GKGCMC	5 cut(s)	2917, 3128, 3244, 4042, 4502
BstSNI	TACGTA	1 cut(s)	3320
BstUI	CGCG	1 cut(s)	2782
BstV2I	GAAGAC	4 cut(s)	2328, 2643, 2858, 4704
BstX2I	RGATCY	2 cut(s)	1009, 4019
BstXI	CCANNNNNNTGG	2 cut(s)	2575, 3385
BstYI	RGATCY	2 cut(s)	1009, 4019
BstZI	CGGCCG	1 cut(s)	214
Bsu15I	ATCGAT	2 cut(s)	1328, 4316
BsuI	GTATCC	1 cut(s)	757
BsuTUI	ATCGAT	2 cut(s)	1328, 4316
BtgI	CCRYGG	4 cut(s)	43, 700, 3128, 3285
BtrI	CACGTC	1 cut(s)	1524
BtsI	GCAGTG	3 cut(s)	1305, 4042, 4143
BtsIMutI	CAGTG	8 cut(s)	1009, 1305, 1517, 1577, 2380, 3385, 4042, 4143
BveI	ACCTGC	1 cut(s)	1789
Cac8I	GCNNGC	9 cut(s)	1108, 1602, 2378, 2659, 2663, 3561, 3597, 3913, 4116
CaiI	CAGNNNCTG	4 cut(s)	1378, 1628, 2522, 4563
CciI	TCATGA	2 cut(s)	2985, 3097
CfoI	GCGC	2 cut(s)	237, 1241
Cfr10I	RCCGGY	1 cut(s)	4438
Cfr13I	GGNCC	6 cut(s)	1105, 2899, 3124, 3125, 3333, 3972
Cfr9I	CCCGGG	1 cut(s)	3975
ClaI	ATCGAT	2 cut(s)	1328, 4316
CseI	GACGC	3 cut(s)	2788, 3221, 4175
CspAI	ACCGGT	1 cut(s)	4438
CspCI	CAANNNNNGTGG	2 cut(s)	4673, 4708
DinI	GGCGCC	1 cut(s)	236
DraI	TTTAAA	5 cut(s)	1407, 2233, 2862, 3369, 4719
DrdI	GACNNNNNNGTC	1 cut(s)	1528
DseDI	GACNNNNNNGTC	1 cut(s)	1528
EaeI	YGGCCR	6 cut(s)	214, 1512, 1676, 2367, 2746, 2885
EagI	CGGCCG	1 cut(s)	214
Eam1104I	CTCTTC	6 cut(s)	156, 1297, 1338, 2820, 3772, 4532
EarI	CTCTTC	6 cut(s)	156, 1297, 1338, 2820, 3772, 4532
EclXI	CGGCCG	1 cut(s)	214
Eco105I	TACGTA	1 cut(s)	3320
Eco130I	CCWWGG	7 cut(s)	43, 157, 1132, 2159, 3128, 3285, 3465
Eco147I	AGGCCT	1 cut(s)	3575
Eco24I	GRGCYC	1 cut(s)	3128
Eco31I	GGTCTC	2 cut(s)	151, 884
Eco32I	GATATC	1 cut(s)	1206
Eco47I	GGWCC	1 cut(s)	2899
Eco47III	AGCGCT	1 cut(s)	1240
Eco52I	CGGCCG	1 cut(s)	214
Eco57I	CTGAAG	9 cut(s)	876, 1034, 1742, 2322, 2571, 2655, 2685, 3756, 4911
Eco72I	CACGTG	1 cut(s)	4819
Eco88I	CYCGRG	1 cut(s)	3975
EcoNI	CCTNNNNNAGG	2 cut(s)	185, 455
EcoO109I	RGGNCCY	2 cut(s)	2899, 3972
EcoRI	GAATTC	5 cut(s)	1419, 2512, 3520, 3649, 4777
EcoRII	CCWGG	5 cut(s)	803, 893, 1726, 3554, 4118
EcoRV	GATATC	1 cut(s)	1206
EcoT14I	CCWWGG	7 cut(s)	43, 157, 1132, 2159, 3128, 3285, 3465
EcoT22I	ATGCAT	4 cut(s)	1157, 2712, 3181, 4617
EcoT38I	GRGCYC	1 cut(s)	3128
EgeI	GGCGCC	1 cut(s)	236
EheI	GGCGCC	1 cut(s)	236
ErhI	CCWWGG	7 cut(s)	43, 157, 1132, 2159, 3128, 3285, 3465
FalI	AAGNNNNNCTT	4 cut(s)	2960, 2992, 3048, 3080
FaqI	GGGAC	8 cut(s)	1473, 1731, 2399, 2533, 2637, 3126, 4862, 4928
FauI	CCCGC	2 cut(s)	1115, 3920
FbaI	TGATCA	3 cut(s)	358, 1549, 4669
FriOI	GRGCYC	1 cut(s)	3128
GlaI	GCGC	2 cut(s)	236, 1240
GsaI	CCCAGC	3 cut(s)	534, 3486, 4134
GsuI	CTGGAG	1 cut(s)	4371
HaeII	RGCGCY	2 cut(s)	238, 1242
HapII	CCGG	7 cut(s)	57, 217, 1082, 1900, 2111, 3976, 4439
HgaI	GACGC	3 cut(s)	2788, 3221, 4175
HhaI	GCGC	2 cut(s)	237, 1241
Hin1I	GRCGYC	1 cut(s)	235
Hin6I	GCGC	2 cut(s)	235, 1239
HinP1I	GCGC	2 cut(s)	235, 1239
HincII	GTYRAC	3 cut(s)	2778, 3921, 4659
HindII	GTYRAC	3 cut(s)	2778, 3921, 4659
HindIII	AAGCTT	1 cut(s)	4341
HpaII	CCGG	7 cut(s)	57, 217, 1082, 1900, 2111, 3976, 4439
HphI	GGTGA	6 cut(s)	688, 785, 983, 1096, 2129, 4772
Hpy99I	CGWCG	1 cut(s)	4413
HpyCH4IV	ACGT	6 cut(s)	1434, 1523, 3319, 4408, 4606, 4818
HpySE526I	ACGT	6 cut(s)	1434, 1523, 3319, 4408, 4606, 4818
Hsp92I	GRCGYC	1 cut(s)	235
HspAI	GCGC	2 cut(s)	235, 1239
KasI	GGCGCC	1 cut(s)	234
KpnI	GGTACC	3 cut(s)	1959, 2248, 4911
Ksp22I	TGATCA	3 cut(s)	358, 1549, 4669
LguI	GCTCTTC	1 cut(s)	3772
LmnI	GCTCC	5 cut(s)	204, 1375, 2408, 2959, 3743
MaeII	ACGT	6 cut(s)	1434, 1523, 3319, 4408, 4606, 4818
MaeIII	GTNAC	9 cut(s)	560, 773, 2117, 2178, 2524, 3307, 3961, 4278, 4559
MfeI	CAATTG	2 cut(s)	4883, 4969
MflI	RGATCY	2 cut(s)	1009, 4019
MhlI	GDGCHC	6 cut(s)	696, 2917, 3128, 3244, 4042, 4502
MlsI	TGGCCA	3 cut(s)	2369, 2748, 2887
MluI	ACGCGT	1 cut(s)	2780
MluNI	TGGCCA	3 cut(s)	2369, 2748, 2887
Mly113I	GGCGCC	1 cut(s)	235
MlyI	GAGTC	5 cut(s)	1495, 1651, 2285, 3145, 3362
MmeI	TCCRAC	5 cut(s)	1479, 2068, 3198, 3381, 3416
Mox20I	TGGCCA	3 cut(s)	2369, 2748, 2887
Mph1103I	ATGCAT	4 cut(s)	1157, 2712, 3181, 4617
MroXI	GAANNNNTTC	3 cut(s)	32, 286, 927
MscI	TGGCCA	3 cut(s)	2369, 2748, 2887
MslI	CAYNNNNRTG	8 cut(s)	1115, 1522, 1557, 2573, 2990, 3066, 4249, 4761
Msp20I	TGGCCA	3 cut(s)	2369, 2748, 2887
MspA1I	CMGCKG	5 cut(s)	689, 968, 2561, 3078, 3251
MspI	CCGG	7 cut(s)	57, 217, 1082, 1900, 2111, 3976, 4439
MspR9I	CCNGG	7 cut(s)	805, 895, 1728, 3556, 3976, 3977, 4120
MunI	CAATTG	2 cut(s)	4883, 4969
Mva1269I	GAATGC	5 cut(s)	532, 1155, 2018, 2282, 4642
MvaI	CCWGG	5 cut(s)	805, 895, 1728, 3556, 4120
MvnI	CGCG	1 cut(s)	2782
NarI	GGCGCC	1 cut(s)	235
NciI	CCSGG	2 cut(s)	3976, 3977
NcoI	CCATGG	3 cut(s)	43, 3128, 3285
NheI	GCTAGC	1 cut(s)	2657
NlaIV	GGNNCC	9 cut(s)	206, 236, 1615, 1957, 2246, 3126, 3974, 4835, 4909
NmeAIII	GCCGAG	1 cut(s)	192
NmuCI	GTSAC	7 cut(s)	560, 773, 2117, 2178, 2524, 3307, 4278
NsiI	ATGCAT	4 cut(s)	1157, 2712, 3181, 4617
NspI	RCATGY	6 cut(s)	2469, 2669, 3269, 4118, 4164, 4968
NspV	TTCGAA	1 cut(s)	4007
OliI	CACNNNNGTG	1 cut(s)	1522
PaeI	GCATGC	1 cut(s)	4118
PagI	TCATGA	2 cut(s)	2985, 3097
PceI	AGGCCT	1 cut(s)	3575
PciI	ACATGT	2 cut(s)	2465, 3265
PciSI	GCTCTTC	1 cut(s)	3772
PctI	GAATGC	5 cut(s)	532, 1155, 2018, 2282, 4642
PdmI	GAANNNNTTC	3 cut(s)	32, 286, 927
Pfl23II	CGTACG	1 cut(s)	72
PflFI	GACNNNGTC	1 cut(s)	1889
PflMI	CCANNNNNTGG	2 cut(s)	584, 1998
PinAI	ACCGGT	1 cut(s)	4438
PleI	GAGTC	5 cut(s)	1494, 1650, 2284, 3144, 3361
PluTI	GGCGCC	1 cut(s)	238
PmaCI	CACGTG	1 cut(s)	4819
PmlI	CACGTG	1 cut(s)	4819
PpsI	GAGTC	5 cut(s)	1494, 1650, 2284, 3144, 3361
Ppu21I	YACGTR	2 cut(s)	3320, 4819
PpuMI	RGGWCCY	1 cut(s)	2899
PscI	ACATGT	2 cut(s)	2465, 3265
PshBI	ATTAAT	3 cut(s)	918, 1056, 1857
PsiI	TTATAA	1 cut(s)	4032
Psp5II	RGGWCCY	1 cut(s)	2899
Psp6I	CCWGG	5 cut(s)	803, 893, 1726, 3554, 4118
PspCI	CACGTG	1 cut(s)	4819
PspFI	CCCAGC	3 cut(s)	530, 3482, 4130
PspGI	CCWGG	5 cut(s)	803, 893, 1726, 3554, 4118
PspLI	CGTACG	1 cut(s)	72
PspN4I	GGNNCC	9 cut(s)	206, 236, 1615, 1957, 2246, 3126, 3974, 4835, 4909
PspOMI	GGGCCC	1 cut(s)	3124
PspPI	GGNCC	6 cut(s)	1105, 2899, 3124, 3125, 3333, 3972
PspPPI	RGGWCCY	1 cut(s)	2899
PstI	CTGCAG	2 cut(s)	1300, 3111
PstNI	CAGNNNCTG	4 cut(s)	1378, 1628, 2522, 4563
PsuI	RGATCY	2 cut(s)	1009, 4019
PsyI	GACNNNGTC	1 cut(s)	1889
PvuII	CAGCTG	4 cut(s)	689, 968, 2561, 3078
RseI	CAYNNNNRTG	8 cut(s)	1115, 1522, 1557, 2573, 2990, 3066, 4249, 4761
SapI	GCTCTTC	1 cut(s)	3772
Sau96I	GGNCC	6 cut(s)	1105, 2899, 3124, 3125, 3333, 3972
SchI	GAGTC	5 cut(s)	1495, 1651, 2285, 3145, 3362
ScrFI	CCNGG	7 cut(s)	805, 895, 1728, 3556, 3976, 3977, 4120
SduI	GDGCHC	6 cut(s)	696, 2917, 3128, 3244, 4042, 4502
SfcI	CTRYAG	3 cut(s)	1215, 1296, 3107
SfoI	GGCGCC	1 cut(s)	236
SfuI	TTCGAA	1 cut(s)	4007
SinI	GGWCC	1 cut(s)	2899
SmaI	CCCGGG	1 cut(s)	3977
SmiI	ATTTAAAT	1 cut(s)	1407
SmiMI	CAYNNNNRTG	8 cut(s)	1115, 1522, 1557, 2573, 2990, 3066, 4249, 4761
SmlI	CTYRAG	1 cut(s)	3947
SmoI	CTYRAG	1 cut(s)	3947
SnaBI	TACGTA	1 cut(s)	3320
SpeI	ACTAGT	1 cut(s)	3581
SphI	GCATGC	1 cut(s)	4118
SseBI	AGGCCT	1 cut(s)	3575
SspDI	GGCGCC	1 cut(s)	234
SspI	AATATT	2 cut(s)	2893, 3203
StuI	AGGCCT	1 cut(s)	3575
StyD4I	CCNGG	7 cut(s)	803, 893, 1726, 3554, 3974, 3975, 4118
StyI	CCWWGG	7 cut(s)	43, 157, 1132, 2159, 3128, 3285, 3465
SwaI	ATTTAAAT	1 cut(s)	1407
TaiI	ACGT	6 cut(s)	1437, 1526, 3322, 4411, 4609, 4821
TaqI	TCGA	8 cut(s)	96, 138, 262, 498, 729, 1328, 4007, 4316
TatI	WGTACW	3 cut(s)	3488, 4237, 4273
TauI	GCSGC	5 cut(s)	80, 83, 197, 210, 1517
TscAI	CASTG	8 cut(s)	1009, 1305, 1524, 1584, 2387, 3385, 4049, 4143
TseFI	GTSAC	7 cut(s)	560, 773, 2117, 2178, 2524, 3307, 4278
Tsp45I	GTSAC	7 cut(s)	560, 773, 2117, 2178, 2524, 3307, 4278
TspGWI	ACGGA	2 cut(s)	717, 2932
TspMI	CCCGGG	1 cut(s)	3975
TspRI	CASTG	8 cut(s)	1009, 1305, 1524, 1584, 2387, 3385, 4049, 4143
Tth111I	GACNNNGTC	1 cut(s)	1889
Van91I	CCANNNNNTGG	2 cut(s)	584, 1998
VneI	GTGCAC	3 cut(s)	2913, 3240, 4038
VpaK11BI	GGWCC	1 cut(s)	2899
VspI	ATTAAT	3 cut(s)	918, 1056, 1857
XagI	CCTNNNNNAGG	2 cut(s)	185, 455
XbaI	TCTAGA	3 cut(s)	428, 1411, 3683
XceI	RCATGY	6 cut(s)	2469, 2669, 3269, 4118, 4164, 4968
XcmI	CCANNNNNNNNNTGG	1 cut(s)	811
XmaI	CCCGGG	1 cut(s)	3975
XmaJI	CCTAGG	1 cut(s)	1132
XmnI	GAANNNNTTC	3 cut(s)	32, 286, 927
Zsp2I	ATGCAT	4 cut(s)	1157, 2712, 3181, 4617

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.