Rmu_sc0000446.1_g000011

DDT domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000446.1
OG0003576
Physical Location & Seq
Forward (+)
33641 .. 44445
10805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000446.1_g000011.1.cds

Sequence Viewer

Length: 4569 bp
ctggccgaccccggcccggcccgaaagcccaagaaacgacgccgtacaggtaattctgatcccaattacaggggggaggatgagcatgctagcagtaattctagtccagaaaaggagcaggggattgatttgaatgcgggctttaattttagcttaaatgatgctgctgacctgaatgttgttggtgaggagagagattgtattgatttgaatttggatgccagcggttgtgattctgttgacaatttcgatgggcctagtttgggccaagggaccaagcggaggggttgcgatttcgatttgaatttggaggttgctgacgagggggacagtgaagacaagttcaaaattagtcccgagtttgagaggattgaagagagtcgaagcagcggagacgcccaaggaaatgttgctgaggatgggaatgttaatgagacccggaagcaagtgtgtattgatattaatcaagaagttcccttgaatggcattgagtgtgctgctgctggggaaggactcggtaatgagaaccgctggtccactggagatatgaaggtcgaatcttctcttggggatttgaatgctagtgctgttaaggataacggtatggtggaagatggtccttctgaagctgctactccggtgactcatgggcacccaggcgattcagcaagcccaagcattcaaaaaagtagccgaaggaagagaagaaagctaccggataatttgatatcgaatactgcagagacagttttgaggagaagttctcgcagaggatctgctcggaatcatgtctccgttgcatcatctgctgttagtaatcctgtgtcctcttctgcagctactgcagtaacagaggacgtaccggtgatttctgccagtgaagaagccgacaagcctagtgttgcccctcagaagctcgaattacctccttcgtcacaacatttgaatttagagggcattcccgtacttgaccttttttctatttatgcctgtttgagatcatttagtacaatattgtttctgagtcccttcaagttggaggattttgtgagagcactgcaatgcaactctcctagtttgttatttgattctgttcatgtttctattttgcaaacgctaagaaagcatttggagtccctttcaaatgaaggttccgaatctgcttctgattgtctaaggagtcttaattgggatttcctggacttaattacgtggccagttttcatggttgagtactttctgattcattgttcaggactgaaacctggatttgacctcggtcactttaagctattaaaaagtgactactacagtcagcctgcttctctaaaggttgagattctttgctgtctatgtgatgatttgatagaaggggaagccataaagtcggagattaatagaaggtgttcgacatctgaacatgacatgggttttgatcgtgatgtaaactttgatgtctgcaagaaaaggaaggcttctgttcaaattgctggtagctccagtttgaacgatgaaaatgttgatgaaacccctgactggaatagtgatgaatgttgcctctgtaagatggaagggaacttaatatgctgtgatggctgtccagcagcttaccattcaagatgtgtaggagttgtcaatgatcttttgccggagggtgattggtattgccctgaatgtatgattgataggcacaaaccttggatgaaactccgcaagtcactgcgaggtgcagagctattaggaattgatcctcatggtcggctatattttaaaagctgcggctacctgttggtatctggtttttgtgatgatgaatctgcatttaattactatcacagagatgatctaaacaagattattgaagtgctaaggtcttcagaattttcctatggtgggatattattgggaatctataagcactgggacattcctactacctttaatggagcaattagtagcaatcccctggaccaattggaattttcagaaacaggtgatgctaaaaatgagacgcctgaggatagaaaactgcaagagaagttttgtaatctcgtttctgatgttccaaattctgttcaagtgctgagaaggactgtcattcaggatgattcaaataatcttgctgatatttcaaatcaaccagatttggtcagaaaacttcatccggaagactgttctttgacatctactcgcttagatgtcagacaagagagcaacggttctattgatcttggaaaaatttcatctgctataaccactagaaaagagggcacttcagaagtggattgtggaattgattacatgaactactacagttttggccaaattgcttcatctgttgcagaggagttgtctaaatcatcagaaaagaataaggaaggtgcactcaatacagtagatgaaataatatctgcgcagatgaagaccattataaaaaaatcttccaagttttcctggccaaatattgagaacttaaatatagatgtgcagaaagagaaatgtgggtggtgcttttcttgcaaatatcctgctgatgatagggattgcttgcttatcatgagtaagctgcctttactggatgtttccaagactgatgtagttggacttcaatttaaaaaaattcccaaagatcatcttaatgatgtgagctgtcaaattttgtccattcatgatcgccttcttgggcttctgttgggtccgtggttgaacccccatcatacagaatgctggaggaatagtcttcttaatgcatccgatcttgcctctgttaagcatttactgctccttctagtggaaaatttgcatcatcgtacattgtccgcagattggttgaaacatgtggactctgttgttaccatgggttcagcttctcatgttgtgacttcattacgtgcatactcaaagaacatgaacagcagaaagaggcccaagttttctgatattgattcaaatccatcttcaaatgccggcagtggattgggaatgttttggtggaggggtggcaggctttctcgtcaggtgtttggttggaagattttgcctcgctccttgacttctaaggctgccagacaaggtgggtgcacaaaaataatgggaatattgtatcctgagaattcagaatatgcaaagagaagcaaatatattgcctggcgagctactgttgaggtgtcgactagtgcagaacagcttgctttgcaggttagggagctttattcaaacattaggtgggatgatattgagaacacacacccgctgcctacattggacaaggaatctacaaaatcaatcaagcttttcaggaaagttattgtccgcaggaagtgctcagaaaagggagtggttaagtatcttcttgattttggcaagagaagagcaatccccgacattgtcaggaaacatggttctgtgcttgaagaaccatctaatgagaggaaaaagtattggcttgaagaatcatatcttcctctgcatcttttgaaaaattttgaggagagaagaattgcccgcaagtctagtgatgggaagtctggtaaagttcttgcagttggtagagtaatgcggcccccagatgaaaagggatttgcttatttgttttccaaagcagaaagatcagagtattataagtgtggacactgccacaaagatgtcctgataagggaagctgtgagttgtcaattgtgcagaggatttttccacaaaaggcatgcgaagaagtctgctggggccattgtttccgagtgtacgtatacatgccaccgatgccagaatggggtctctgcgaagattgatacaaaaagagggaaagctgataaaaagagaggaaaagttggtatgaaaagaaggaaagttgaaacaaaaatagagaaagtacagtcacagaaatgtaaaaattctcaaactgatcgtagatcactgcgtttgagaagcaaaagaaaagctttagctggaggaagacaggtacaattgaaaaatagcaaaaaggttcctgtaagtgtacctttacgccgctctccaaggaaagctaaatgcttgacgttgcaaaataaaaagcatagcaaacgcaagaaagggaaacaaagcaaatccaagaagcggacgtataagaaaccaaagacagggaccttgtgtcagaagaagagaacacaagtttatcgtagttactggctaaatggtctgcagttgtctagaaagccaaatgataaacgtgttacgctttttagggagaaaaaactacttgctcactctggctgttcttctatcgttctcggtcaactcaaatgccagctctgttgtgaagcagaatatgcatctactttagattatatcgggtgtgaactatgtggagagtggtttcatggagaggcttttgggctggattcagagaacattgataagctcattggattcaggtgccatgtgtgtcgtaaaacagaacctcctgtctgcccccatctggtagttgtgaaaactgatgtgtcccaattggctgaagctcaaactgatggttctgttgattgttcagaggaagtacctaatgcagtcccgactttgagtgaggtatattcatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0006139 GO:0006325 GO:0006338 GO:0006351 GO:0006355 GO:0006357 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0008134 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016043 GO:0016070 GO:0016589 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031010 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0046483 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0060255 GO:0060322 GO:0065007 GO:0070013 GO:0070603 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902494 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT PHD WHIM1

Protein Analysis

1522

Amino Acids

170.14

Weight (kDa)

8.58

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2432, 3621
Acc16I TGCGCA 1 cut(s) 2415
Acc36I ACCTGC 1 cut(s) 3187
AccB1I GGYRCC 2 cut(s) 651, 4410
AccBSI CCGCTC 1 cut(s) 4008
AccI GTMKAC 2 cut(s) 3170, 3746
AccIII TCCGGA 1 cut(s) 2164
AclWI GGATC 3 cut(s) 53, 781, 1742
AcoI YGGCCR 4 cut(s) 3, 1214, 2320, 2456
AcuI CTGAAG 4 cut(s) 645, 1860, 2259, 4509
AcyI GRCGYC 3 cut(s) 40, 396, 2012
AflIII ACRYGT 2 cut(s) 2836, 4204
AgeI ACCGGT 1 cut(s) 862
AhdI GACNNNNNGTC 1 cut(s) 4125
AhlI ACTAGT 1 cut(s) 3173
AjnI CCWGG 6 cut(s) 655, 1197, 1264, 1965, 2453, 3146
AjuI GAANNNNNNNTTGG 2 cut(s) 549, 581
AloI GAACNNNNNNTCC 4 cut(s) 518, 550, 3385, 3417
Alw21I GWGCWC 4 cut(s) 1057, 2386, 3083, 3326
Alw26I GTCTC 6 cut(s) 387, 428, 737, 796, 2003, 3778
Alw44I GTGCAC 2 cut(s) 2382, 3079
AlwI GGATC 3 cut(s) 53, 781, 1742
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 1 cut(s) 356
Aor13HI TCCGGA 1 cut(s) 2164
ApaLI GTGCAC 2 cut(s) 2382, 3079
ArsI GACNNNNNNTTYG 2 cut(s) 1415, 1447
AseI ATTAAT 2 cut(s) 462, 1395
AsiGI ACCGGT 1 cut(s) 862
Asp700I GAANNNNTTC 2 cut(s) 1405, 2239
AspLEI GCGC 1 cut(s) 2416
AsuC2I CCSGG 3 cut(s) 12, 17, 439
AsuHPI GGTGA 5 cut(s) 197, 652, 877, 1667, 2006
AsuNHI GCTAGC 1 cut(s) 89
AvaI CYCGRG 1 cut(s) 356
AvaII GGWCC 6 cut(s) 273, 534, 617, 1969, 2696, 4119
AxyI CCTNAGG 1 cut(s) 2016
BaeGI GKGCMC 4 cut(s) 654, 2273, 2386, 3083
BalI TGGCCA 3 cut(s) 1216, 2322, 2458
BanI GGYRCC 2 cut(s) 651, 4410
BarI GAAGNNNNNNTAC 2 cut(s) 2589, 2621
BbsI GAAGAC 6 cut(s) 342, 1866, 2175, 2429, 2732, 3958
Bbv12I GWGCWC 4 cut(s) 1057, 2386, 3083, 3326
BbvCI CCTCAGC 1 cut(s) 414
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 6 cut(s) 657, 1199, 1266, 1967, 2455, 3148
BciVI GTATCC 1 cut(s) 3114
BcnI CCSGG 3 cut(s) 12, 17, 439
BcoDI GTCTC 6 cut(s) 387, 428, 737, 796, 2003, 3778
BcuI ACTAGT 1 cut(s) 3173
BfmI CTRYAG 6 cut(s) 738, 834, 843, 1309, 2311, 4175
BfuAI ACCTGC 1 cut(s) 3187
BfuI GTATCC 1 cut(s) 3114
BmcAI AGTACT 1 cut(s) 1235
Bme1390I CCNGG 9 cut(s) 12, 17, 439, 657, 1199, 1266, 1967, 2455, 3148
Bme18I GGWCC 6 cut(s) 273, 534, 617, 1969, 2696, 4119
BmeRI GACNNNNNGTC 1 cut(s) 4125
BmeT110I CYCGRG 1 cut(s) 356
BmiI GGNNCC 9 cut(s) 274, 653, 1153, 2697, 3563, 3724, 3984, 4120, 4412
BmrFI CCNGG 9 cut(s) 12, 17, 439, 657, 1199, 1266, 1967, 2455, 3148
BmrI ACTGGG 1 cut(s) 1930
BmsI GCATC 9 cut(s) 151, 208, 809, 1987, 2759, 2812, 3478, 3749, 4316
BmtI GCTAGC 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 1930
BoxI GACNNNNGTC 1 cut(s) 1278
BpiI GAAGAC 6 cut(s) 342, 1866, 2175, 2429, 2732, 3958
BplI GAGNNNNNCTC 2 cut(s) 748, 780
BpmI CTGGAG 4 cut(s) 561, 1483, 2749, 3966
Bpu10I CCTNAGC 2 cut(s) 414, 1868
BpuMI CCSGG 3 cut(s) 12, 17, 439
BsaAI YACGTR 3 cut(s) 1212, 2891, 3744
BsaBI GATNNNNATC 2 cut(s) 462, 2949
BsaHI GRCGYC 3 cut(s) 40, 396, 2012
BsaI GGTCTC 2 cut(s) 428, 3778
BsaWI WCCGGW 4 cut(s) 637, 715, 862, 2164
BsaXI ACNNNNNCTCC 4 cut(s) 2722, 2752, 4420, 4450
Bse118I RCCGGY 2 cut(s) 862, 2966
Bse1I ACTGG 8 cut(s) 544, 876, 1217, 1500, 1541, 1925, 2580, 4166
Bse21I CCTNAGG 1 cut(s) 2016
Bse3DI GCAATG 1 cut(s) 1067
Bse8I GATNNNNATC 2 cut(s) 462, 2949
BseAI TCCGGA 1 cut(s) 2164
BseBI CCWGG 6 cut(s) 657, 1199, 1266, 1967, 2455, 3148
BseGI GGATG 9 cut(s) 85, 223, 424, 1707, 2110, 2161, 2584, 2750, 3235
BseJI GATNNNNATC 2 cut(s) 462, 2949
BseMI GCAATG 1 cut(s) 1067
BseMII CTCAG 7 cut(s) 405, 923, 1013, 2007, 2075, 3099, 3339
BseNI ACTGG 8 cut(s) 544, 876, 1217, 1500, 1541, 1925, 2580, 4166
BseRI GAGGAG 4 cut(s) 203, 769, 2360, 3503
BseSI GKGCMC 4 cut(s) 654, 2273, 2386, 3083
BseYI CCCAGC 2 cut(s) 503, 3719
BsgI GTGCAG 4 cut(s) 1749, 2507, 3198, 3700
BshNI GGYRCC 2 cut(s) 651, 4410
BshTI ACCGGT 1 cut(s) 862
BsiHKAI GWGCWC 4 cut(s) 1057, 2386, 3083, 3326
BsiHKCI CYCGRG 1 cut(s) 356
BsiSI CCGG 9 cut(s) 12, 17, 439, 638, 716, 863, 1649, 2165, 2967
BslFI GGGAC 9 cut(s) 286, 339, 341, 1011, 1120, 1937, 4132, 4462, 4526
BsmAI GTCTC 6 cut(s) 387, 428, 737, 796, 2003, 3778
BsmBI CGTCTC 2 cut(s) 387, 2003
BsmFI GGGAC 9 cut(s) 286, 339, 341, 1011, 1120, 1937, 4132, 4462, 4526
BsmI GAATGC 5 cut(s) 139, 583, 678, 957, 2729
Bso31I GGTCTC 2 cut(s) 428, 3778
BsoBI CYCGRG 1 cut(s) 356
Bsp1286I GDGCHC 6 cut(s) 654, 1057, 2273, 2386, 3083, 3326
Bsp13I TCCGGA 1 cut(s) 2164
Bsp19I CCATGG 1 cut(s) 2856
BspCNI CTCAG 7 cut(s) 406, 922, 1014, 2008, 2076, 3100, 3338
BspEI TCCGGA 1 cut(s) 2164
BspHI TCATGA 2 cut(s) 2556, 2668
BspLI GGNNCC 9 cut(s) 274, 653, 1153, 2697, 3563, 3724, 3984, 4120, 4412
BspMAI CTGCAG 4 cut(s) 742, 838, 847, 4179
BspMI ACCTGC 1 cut(s) 3187
BspOI GCTAGC 1 cut(s) 93
BspPI GGATC 3 cut(s) 53, 781, 1742
BspQI GCTCTTC 1 cut(s) 3364
BspT107I GGYRCC 2 cut(s) 651, 4410
BspTNI GGTCTC 2 cut(s) 428, 3778
BsrBI CCGCTC 1 cut(s) 4008
BsrDI GCAATG 1 cut(s) 1067
BsrFI RCCGGY 2 cut(s) 862, 2966
BsrI ACTGG 8 cut(s) 544, 876, 1217, 1500, 1541, 1925, 2580, 4166
BssAI RCCGGY 2 cut(s) 862, 2966
BssNAI GTATAC 1 cut(s) 3747
BssNI GRCGYC 3 cut(s) 40, 396, 2012
BssT1I CCWWGG 5 cut(s) 268, 400, 1697, 2856, 4013
Bst1107I GTATAC 1 cut(s) 3747
Bst2UI CCWGG 6 cut(s) 657, 1199, 1266, 1967, 2455, 3148
Bst6I CTCTTC 5 cut(s) 369, 695, 835, 3364, 4130
BstACI GRCGYC 3 cut(s) 40, 396, 2012
BstAPI GCANNNNNTGC 5 cut(s) 806, 842, 2779, 3321, 4304
BstBAI YACGTR 3 cut(s) 1212, 2891, 3744
BstDSI CCRYGG 2 cut(s) 2699, 2856
BstF5I GGATG 9 cut(s) 85, 223, 424, 1707, 2110, 2161, 2584, 2750, 3235
BstHHI GCGC 1 cut(s) 2416
BstMAI GTCTC 6 cut(s) 387, 428, 737, 796, 2003, 3778
BstNI CCWGG 6 cut(s) 657, 1199, 1266, 1967, 2455, 3148
BstNSI RCATGY 4 cut(s) 89, 2840, 3707, 3753
BstPAI GACNNNNGTC 1 cut(s) 1278
BstSCI CCNGG 9 cut(s) 10, 15, 437, 655, 1197, 1264, 1965, 2453, 3146
BstSFI CTRYAG 6 cut(s) 738, 834, 843, 1309, 2311, 4175
BstSLI GKGCMC 4 cut(s) 654, 2273, 2386, 3083
BstSNI TACGTA 1 cut(s) 3744
BstV2I GAAGAC 6 cut(s) 342, 1866, 2175, 2429, 2732, 3958
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BstZ17I GTATAC 1 cut(s) 3747
Bsu36I CCTNAGG 1 cut(s) 2016
BsuI GTATCC 1 cut(s) 3114
BtgI CCRYGG 2 cut(s) 2699, 2856
BtsCI GGATG 9 cut(s) 85, 223, 424, 1707, 2110, 2161, 2584, 2750, 3235
BtsI GCAGTG 5 cut(s) 1055, 1718, 2977, 3631, 3911
BtsIMutI CAGTG 9 cut(s) 337, 537, 883, 1055, 1718, 1918, 2977, 3631, 3911
BveI ACCTGC 1 cut(s) 3187
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 2 cut(s) 2556, 2668
CfoI GCGC 1 cut(s) 2416
Cfr10I RCCGGY 2 cut(s) 862, 2966
CseI GACGC 3 cut(s) 48, 404, 2020
CspAI ACCGGT 1 cut(s) 862
DraI TTTAAA 2 cut(s) 1771, 2614
DriI GACNNNNNGTC 1 cut(s) 4125
EaeI YGGCCR 4 cut(s) 3, 1214, 2320, 2456
Eam1104I CTCTTC 5 cut(s) 369, 695, 835, 3364, 4130
Eam1105I GACNNNNNGTC 1 cut(s) 4125
EarI CTCTTC 5 cut(s) 369, 695, 835, 3364, 4130
Eco105I TACGTA 1 cut(s) 3744
Eco130I CCWWGG 5 cut(s) 268, 400, 1697, 2856, 4013
Eco31I GGTCTC 2 cut(s) 428, 3778
Eco32I GATATC 1 cut(s) 729
Eco47I GGWCC 6 cut(s) 273, 534, 617, 1969, 2696, 4119
Eco57I CTGAAG 4 cut(s) 645, 1860, 2259, 4509
Eco81I CCTNAGG 1 cut(s) 2016
Eco88I CYCGRG 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 4119
EcoRI GAATTC 1 cut(s) 3112
EcoRII CCWGG 6 cut(s) 655, 1197, 1264, 1965, 2453, 3146
EcoRV GATATC 1 cut(s) 729
EcoT14I CCWWGG 5 cut(s) 268, 400, 1697, 2856, 4013
EcoT22I ATGCAT 2 cut(s) 2752, 4309
ErhI CCWWGG 5 cut(s) 268, 400, 1697, 2856, 4013
Esp3I CGTCTC 2 cut(s) 387, 2003
FalI AAGNNNNNCTT 8 cut(s) 1459, 1491, 2619, 2651, 3922, 3954, 3982, 4014
FaqI GGGAC 9 cut(s) 286, 339, 341, 1011, 1120, 1937, 4132, 4462, 4526
FauI CCCGC 3 cut(s) 130, 3258, 3512
FblI GTMKAC 2 cut(s) 3170, 3746
FokI GGATG 9 cut(s) 92, 230, 431, 1714, 2117, 2148, 2591, 2737, 3242
FspI TGCGCA 1 cut(s) 2415
GlaI GCGC 1 cut(s) 2415
GsaI CCCAGC 2 cut(s) 507, 3723
GsuI CTGGAG 4 cut(s) 561, 1483, 2749, 3966
HapII CCGG 9 cut(s) 12, 17, 439, 638, 716, 863, 1649, 2165, 2967
HgaI GACGC 3 cut(s) 48, 404, 2020
HhaI GCGC 1 cut(s) 2416
Hin1I GRCGYC 3 cut(s) 40, 396, 2012
Hin6I GCGC 1 cut(s) 2414
HinP1I GCGC 1 cut(s) 2414
HincII GTYRAC 3 cut(s) 241, 3171, 4271
HindII GTYRAC 3 cut(s) 241, 3171, 4271
HindIII AAGCTT 2 cut(s) 3290, 3936
HpaII CCGG 9 cut(s) 12, 17, 439, 638, 716, 863, 1649, 2165, 2967
HphI GGTGA 5 cut(s) 197, 652, 877, 1667, 2006
Hpy99I CGWCG 1 cut(s) 42
HpyCH4IV ACGT 7 cut(s) 858, 1211, 2890, 3743, 4034, 4097, 4204
HpySE526I ACGT 7 cut(s) 858, 1211, 2890, 3743, 4034, 4097, 4204
Hsp92I GRCGYC 3 cut(s) 40, 396, 2012
HspAI GCGC 1 cut(s) 2414
Kpn2I TCCGGA 1 cut(s) 2164
KroI GCCGGC 1 cut(s) 2966
KroNI GCCGGC 1 cut(s) 2968
LguI GCTCTTC 1 cut(s) 3364
LmnI GCTCC 6 cut(s) 115, 1502, 1946, 2787, 3050, 3205
LweI GCATC 9 cut(s) 151, 208, 809, 1987, 2759, 2812, 3478, 3749, 4316
MaeII ACGT 7 cut(s) 858, 1211, 2890, 3743, 4034, 4097, 4204
MbiI CCGCTC 1 cut(s) 4008
MfeI CAATTG 4 cut(s) 1973, 3674, 3962, 4481
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 6 cut(s) 654, 1057, 2273, 2386, 3083, 3326
MlsI TGGCCA 3 cut(s) 1216, 2322, 2458
MluNI TGGCCA 3 cut(s) 1216, 2322, 2458
MlyI GAGTC 7 cut(s) 388, 507, 637, 1033, 1142, 1189, 2837
MmeI TCCRAC 4 cut(s) 1017, 1368, 2581, 3008
Mox20I TGGCCA 3 cut(s) 1216, 2322, 2458
Mph1103I ATGCAT 2 cut(s) 2752, 4309
MroI TCCGGA 1 cut(s) 2164
MroNI GCCGGC 1 cut(s) 2966
MroXI GAANNNNTTC 2 cut(s) 1405, 2239
MscI TGGCCA 3 cut(s) 1216, 2322, 2458
MslI CAYNNNNRTG 5 cut(s) 1060, 1839, 2637, 3642, 3880
Msp20I TGGCCA 3 cut(s) 1216, 2322, 2458
MspA1I CMGCKG 4 cut(s) 225, 390, 531, 3253
MspI CCGG 9 cut(s) 12, 17, 439, 638, 716, 863, 1649, 2165, 2967
MspR9I CCNGG 9 cut(s) 12, 17, 439, 657, 1199, 1266, 1967, 2455, 3148
MunI CAATTG 4 cut(s) 1973, 3674, 3962, 4481
Mva1269I GAATGC 5 cut(s) 139, 583, 678, 957, 2729
MvaI CCWGG 6 cut(s) 657, 1199, 1266, 1967, 2455, 3148
NaeI GCCGGC 1 cut(s) 2968
NciI CCSGG 3 cut(s) 12, 17, 439
NcoI CCATGG 1 cut(s) 2856
NgoMIV GCCGGC 1 cut(s) 2966
NheI GCTAGC 1 cut(s) 89
NlaIV GGNNCC 9 cut(s) 274, 653, 1153, 2697, 3563, 3724, 3984, 4120, 4412
NmuCI GTSAC 7 cut(s) 640, 933, 1280, 1301, 1716, 2878, 3873
NsbI TGCGCA 1 cut(s) 2415
NsiI ATGCAT 2 cut(s) 2752, 4309
NspI RCATGY 4 cut(s) 89, 2840, 3707, 3753
PaeI GCATGC 2 cut(s) 89, 3707
PagI TCATGA 2 cut(s) 2556, 2668
PciI ACATGT 1 cut(s) 2836
PciSI GCTCTTC 1 cut(s) 3364
PctI GAATGC 5 cut(s) 139, 583, 678, 957, 2729
PdiI GCCGGC 1 cut(s) 2968
PdmI GAANNNNTTC 2 cut(s) 1405, 2239
PflFI GACNNNGTC 1 cut(s) 3386
PfoI TCCNGGA 1 cut(s) 1197
PinAI ACCGGT 1 cut(s) 862
PleI GAGTC 7 cut(s) 387, 507, 637, 1032, 1141, 1188, 2837
PpsI GAGTC 7 cut(s) 387, 507, 637, 1032, 1141, 1188, 2837
Ppu21I YACGTR 3 cut(s) 1212, 2891, 3744
PpuMI RGGWCCY 1 cut(s) 4119
PscI ACATGT 1 cut(s) 2836
PshAI GACNNNNGTC 1 cut(s) 1278
PshBI ATTAAT 2 cut(s) 462, 1395
PsiI TTATAA 2 cut(s) 2432, 3621
Psp5II RGGWCCY 1 cut(s) 4119
Psp6I CCWGG 6 cut(s) 655, 1197, 1264, 1965, 2453, 3146
PspFI CCCAGC 2 cut(s) 503, 3719
PspGI CCWGG 6 cut(s) 655, 1197, 1264, 1965, 2453, 3146
PspN4I GGNNCC 9 cut(s) 274, 653, 1153, 2697, 3563, 3724, 3984, 4120, 4412
PspPPI RGGWCCY 1 cut(s) 4119
PstI CTGCAG 4 cut(s) 742, 838, 847, 4179
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 1 cut(s) 773
PsyI GACNNNGTC 1 cut(s) 3386
RseI CAYNNNNRTG 5 cut(s) 1060, 1839, 2637, 3642, 3880
SalI GTCGAC 1 cut(s) 3169
SapI GCTCTTC 1 cut(s) 3364
ScaI AGTACT 1 cut(s) 1235
SchI GAGTC 7 cut(s) 388, 507, 637, 1033, 1142, 1189, 2837
ScrFI CCNGG 9 cut(s) 12, 17, 439, 657, 1199, 1266, 1967, 2455, 3148
SduI GDGCHC 6 cut(s) 654, 1057, 2273, 2386, 3083, 3326
SfaNI GCATC 9 cut(s) 151, 208, 809, 1987, 2759, 2812, 3478, 3749, 4316
SfcI CTRYAG 6 cut(s) 738, 834, 843, 1309, 2311, 4175
SinI GGWCC 6 cut(s) 273, 534, 617, 1969, 2696, 4119
SmiMI CAYNNNNRTG 5 cut(s) 1060, 1839, 2637, 3642, 3880
SnaBI TACGTA 1 cut(s) 3744
SpeI ACTAGT 1 cut(s) 3173
SphI GCATGC 2 cut(s) 89, 3707
SspI AATATT 3 cut(s) 1014, 2464, 3099
StyD4I CCNGG 9 cut(s) 10, 15, 437, 655, 1197, 1264, 1965, 2453, 3146
StyI CCWWGG 5 cut(s) 268, 400, 1697, 2856, 4013
TaiI ACGT 7 cut(s) 861, 1214, 2893, 3746, 4037, 4100, 4207
TaqI TCGA 8 cut(s) 249, 297, 382, 555, 731, 918, 1409, 3170
TaqII GACCGA 2 cut(s) 1268, 4256
TatI WGTACW 3 cut(s) 1007, 1233, 3868
TauI GCSGC 3 cut(s) 1782, 3562, 4008
TscAI CASTG 9 cut(s) 337, 544, 883, 1062, 1725, 1925, 2977, 3638, 3918
TseFI GTSAC 7 cut(s) 640, 933, 1280, 1301, 1716, 2878, 3873
Tsp45I GTSAC 7 cut(s) 640, 933, 1280, 1301, 1716, 2878, 3873
TspGWI ACGGA 2 cut(s) 784, 2688
TspRI CASTG 9 cut(s) 337, 544, 883, 1062, 1725, 1925, 2977, 3638, 3918
Tth111I GACNNNGTC 1 cut(s) 3386
VneI GTGCAC 2 cut(s) 2382, 3079
VpaK11BI GGWCC 6 cut(s) 273, 534, 617, 1969, 2696, 4119
VspI ATTAAT 2 cut(s) 462, 1395
XbaI TCTAGA 1 cut(s) 4184
XceI RCATGY 4 cut(s) 89, 2840, 3707, 3753
XmiI GTMKAC 2 cut(s) 3170, 3746
XmnI GAANNNNTTC 2 cut(s) 1405, 2239
ZrmI AGTACT 1 cut(s) 1235
Zsp2I ATGCAT 2 cut(s) 2752, 4309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.