Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0003620

Physical Location & Seq

Genomic Coordinates Forward (+)

24013270 .. 24015499

Gene Structure 2230 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1175400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGAGGGTATCTTATTTTCTCCTTTTCAAAAGGATTTTTGTTGAGGAATCCATATCATATTACGATTCCAAATTTGTTTTCAAATGTGATAAGGATATGATTCTGTATTGTCTTTTGCATTATGTTGAAGCTGAACATTCTTTCTCCAGTTTGCTTGAGCTTGCAGCTGATAACGACGTCAAGGGCTTTAAGCAGTCTATCAGCGACCCTGCTGTGGTTAATGAGATTGGACTTTGGTATGGTCGCCATAGCGCTTCAAGACGAATGGTTCTTGAGTATAGGACTCCGTTAATGGTCGCTTCTAAGTATGGTAGTGTTGACGTTGTGAAACTGATTCTCTCTCTTTCAAAGGCGGATGTAAACTTCTCTCTCGGCCCAGATAAGACCACTGCCCTTCACTGTGCTGTGTCTGGTGGGTCTGAAAAAGCTGTTGAAACTGTGAAGGTTCTTCTGCAGGCTGGTGCAGATCCCAATGCTACTGATGCCAACGGATGTTTCCCTTTAGATGTTATTGTTGCTCCTCTGAATTGTTCCAATCTGAAGGTTGTGCTTCAAGAGCTCTTACAATATGGTGGTTCAGTCCATGCTTTAAGTGTTAGTTTTGGATTTAGTTCACCATTTCTTTCAACGACTCCGACAAATAATTCCTTGCCATCATCAATCCCTGATTCAACATGTAACACTTATGACATACATGGTTCTTCTACACCTGAAAAGAAAGAACACCCTTTTGATCTGTCACTTCCCAACATCAAGGATAGCATTTATGCCACAGATGAGTTTCGAATGTTTTCATTCAAGATCCGGCCTTGTTCGCGAGCCTATTCACATGATTGGACTGAATGCCCTTTTGTGCATCCTGGTGAGAATGCAAGGAGAAGAGACCCGAGAAAGTTCCAGTATAGTTGTATGCCATGCTTGGATTTTCGCAAGGGGACATGTAGACGTGGAGATTTATGTGAATATGCTCATGGGGTTTTTGAGTGCTGGCTTCATCCAGCACAGTATAAGACTCGACTCTGCAAAGATGGCATGAGTTGCTTGCGTCAGGTTTGCTTCTTTGCCCACAAACCTGAGGAAATGCGACCCTTGTATGTTTCTACTGGATCTGCCATGCCATCCCCTCATTCAGCAACCTCTGCTGCTGCTTTCATGGACATGGCTGCAACTTTGAACGTGCTGCCTGGCTCGACCAAAGCTGTGCAGGCCATGTCACCTCCTTTTACTCCTCCCATGTCTCTTCATGATGGTATTTTCGACTCTTCCATGGCATGGCCTCAACAATATATCCCCGGTTTGTATCTACCAGGCAGCAATATTCAGGCAAGTCGTCTGAGATCTTCTTTTAATGCAAATAATGTTCCTACCGAGGAGGTTAACATGTTGCATAATTTTGAAATGGAACAACAGCAATCATTTCATGATGGGTCTTCCTTCTCGCATTCGTACATCAGTGCTTCCTCAATGAGTCATCCTCGCCGTCAAGAAACCTTGACACCTTCAAATCTCAATGATGTTTTCGCTTCCCATCTCTCATCCCCTCGATTTGCTGACCAACTTGCAAACTCATCTGCTTTCTCATCCTCAAAAATATCTATTGTTCGCAATCAACTCCACCAGCAACAATCAATGTTATCTCCTATCAGAACAAATGTGTTCTCTCCAAGGTTGGTTGACCAGAATCTCTTGCAAGTTCCATTTGGTCTTTCCTCACTGAGGATGATGTCACCTCGAAGCATGGAGCCTCTTTCTCCAATAGGCACTAGGGTCTCTACTCTTGCTCAGCAAGAGAAGCAGAAGCCGCTATACAGCCCCAACTCATGTGATGCTGGATCCAATGGTGTTGGTTCCCCTATGTCTATGTCAAAATGGGAGTCTAGCAGTGCAAAACTAGATTGGTCTGTTCAAACAGATGAGCCGGGTCATTTAAGCAAATTAAGTTATACAGAACACAATGTCAACGAGCCTGATGTTTCATGGATTCAATCTCTGGTGAAGGAATCACCATCTGATGCAAAGGAAATCACATCTGTTCCAGAATCAGGCACACTGCTACCTATCGAGTGTTCTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 zf-CCCH

Protein Analysis

692

Amino Acids

76.61

Weight (kDa)

6.34

Isoelectric Point (pI)

59.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	92 - 161	3.2e-11	Ankyrin repeats (3 copies)
Ank	PF00023	129 - 161	1.5e-06	Ankyrin repeat
zf-CCCH_AtC3H23	PF25512	260 - 293	1.8e-20	AtC3H23-like, CCCH zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003620)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41900
fragaria_vesca	FvH4_7g01060
malus_domestica	MD03G1175400.v1.1 MD12G1068100.v1.1 MD14G1068200.v1.1
prunus_persica	Prupe.4G248900_v2.0.a1 Prupe.7G065800_v2.0.a1 Prupe.7G065800_v2.0.a1 Prupe.7G065800_v2.0.a1
pyrus_communis	pycom03g13290 pycom11g16880 pycom12g06010
rosa_chinensis	RchiOBHm_Chr1g0316051 RchiOBHm_Chr3g0481371
rosa_laevigata	RLG00000023402 RLG00000030707
rosa_multiflora	Rmu_sc0000064.1_g000020 Rmu_sc0002014.1_g000008 Rmu_sc0002531.1_g000041
rosa_roxburghii	Rroxscaffold_6G00400470
rosa_rugosa	Rorug01G0008400 Rorug01G0008400 Rorug01G0008500 Rorug03G0191500 Rorug03G0191600
rosa_samantha	Rh1AG018800 Rh1BG012300 Rh1CG016100 Rh1DG012500 Rh3AG242500 Rh3BG277500 Rh3CG272500 Rh3DG269400
rosa_wichuraiana	Rw1G001200 Rw3G021670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	180
AccB7I	CCANNNNNTGG	1 cut(s)	1272
AccI	GTMKAC	1 cut(s)	943
AccII	CGCG	1 cut(s)	817
AciI	CCGC	2 cut(s)	353, 1802
AclWI	GGATC	5 cut(s)	461, 796, 1114, 1827, 1840
AcsI	RAATTY	1 cut(s)	71
AcuI	CTGAAG	1 cut(s)	560
AcyI	GRCGYC	1 cut(s)	177
AfaI	GTAC	1 cut(s)	1448
AfeI	AGCGCT	1 cut(s)	253
AfiI	CCNNNNNNNGG	4 cut(s)	214, 1272, 1716, 2042
AflIII	ACRYGT	3 cut(s)	674, 938, 1380
AjiI	CACGTC	1 cut(s)	947
AjnI	CCWGG	3 cut(s)	859, 1183, 1306
AjuI	GAANNNNNNNTTGG	4 cut(s)	62, 94, 479, 511
AluBI	AGCT	6 cut(s)	131, 160, 167, 428, 559, 1199
AluI	AGCT	6 cut(s)	131, 160, 167, 428, 559, 1199
Alw21I	GWGCWC	1 cut(s)	561
Alw26I	GTCTC	3 cut(s)	876, 1242, 1774
AlwI	GGATC	5 cut(s)	461, 796, 1114, 1827, 1840
Ama87I	CYCGRG	1 cut(s)	886
Aor51HI	AGCGCT	1 cut(s)	253
AoxI	GGCC	4 cut(s)	373, 806, 1206, 1274
ApeKI	GCWGC	6 cut(s)	164, 1142, 1145, 1163, 1180, 1311
ApoI	RAATTY	1 cut(s)	71
ArsI	GACNNNNNNTTYG	2 cut(s)	1859, 1891
Asp700I	GAANNNNTTC	1 cut(s)	790
AspLEI	GCGC	1 cut(s)	254
AspS9I	GGNCC	1 cut(s)	374
AsuC2I	CCSGG	2 cut(s)	1293, 1920
AsuHPI	GGTGA	6 cut(s)	606, 875, 1206, 1719, 1995, 2005
AsuII	TTCGAA	1 cut(s)	784
AvaI	CYCGRG	1 cut(s)	886
AxyI	CCTNAGG	1 cut(s)	1074
BamHI	GGATCC	1 cut(s)	1832
BanII	GRGCYC	1 cut(s)	561
BarI	GAAGNNNNNNTAC	2 cut(s)	1790, 1822
BbsI	GAAGAC	1 cut(s)	1422
Bbv12I	GWGCWC	1 cut(s)	561
BbvI	GCAGC	6 cut(s)	176, 1129, 1132, 1150, 1167, 1323
BccI	CCATC	7 cut(s)	661, 1022, 1126, 1241, 1418, 1536, 2014
BceAI	ACGGC	1 cut(s)	1464
BciT130I	CCWGG	3 cut(s)	861, 1185, 1308
BcnI	CCSGG	2 cut(s)	1293, 1920
BcoDI	GTCTC	3 cut(s)	876, 1242, 1774
BfaI	CTAG	3 cut(s)	1764, 1878, 1892
BfmI	CTRYAG	1 cut(s)	452
BfoI	RGCGCY	1 cut(s)	255
BglII	AGATCT	1 cut(s)	1337
BisI	GCNGC	7 cut(s)	165, 1143, 1146, 1164, 1181, 1312, 1802
BlpI	GCTNAGC	1 cut(s)	1782
BlsI	GCNGC	7 cut(s)	166, 1144, 1147, 1165, 1182, 1313, 1803
Bme1390I	CCNGG	5 cut(s)	861, 1185, 1293, 1308, 1920
BmeT110I	CYCGRG	1 cut(s)	886
BmgBI	CACGTC	1 cut(s)	947
BmgT120I	GGNCC	1 cut(s)	374
BmiI	GGNNCC	3 cut(s)	1743, 1834, 1849
BmrFI	CCNGG	5 cut(s)	861, 1185, 1293, 1308, 1920
BmsI	GCATC	4 cut(s)	472, 865, 1816, 2002
BpiI	GAAGAC	1 cut(s)	1422
BplI	GAGNNNNNCTC	2 cut(s)	1459, 1491
BpmI	CTGGAG	1 cut(s)	130
Bpu1102I	GCTNAGC	1 cut(s)	1782
Bpu14I	TTCGAA	1 cut(s)	784
BpuEI	CTTGAG	2 cut(s)	176, 293
BpuMI	CCSGG	2 cut(s)	1293, 1920
BsaHI	GRCGYC	1 cut(s)	177
BsaI	GGTCTC	2 cut(s)	876, 1774
BsaJI	CCNNGG	4 cut(s)	1266, 1291, 1368, 1664
Bsc4I	CCNNNNNNNGG	4 cut(s)	214, 1272, 1716, 2042
Bse1I	ACTGG	3 cut(s)	147, 898, 1108
Bse21I	CCTNAGG	1 cut(s)	1074
BseBI	CCWGG	3 cut(s)	861, 1185, 1308
BseDI	CCNNGG	4 cut(s)	1266, 1291, 1368, 1664
BseGI	GGATG	9 cut(s)	361, 497, 856, 994, 1118, 1471, 1535, 1580, 1725
BseLI	CCNNNNNNNGG	4 cut(s)	214, 1272, 1716, 2042
BseMII	CTCAG	4 cut(s)	1065, 1325, 1706, 1796
BseNI	ACTGG	3 cut(s)	147, 898, 1108
BseRI	GAGGAG	3 cut(s)	510, 1218, 1385
BseXI	GCAGC	6 cut(s)	176, 1129, 1132, 1150, 1167, 1323
BsgI	GTGCAG	2 cut(s)	483, 1223
Bsh1236I	CGCG	1 cut(s)	817
BshFI	GGCC	4 cut(s)	375, 808, 1208, 1276
BsiHKAI	GWGCWC	1 cut(s)	561
BsiHKCI	CYCGRG	1 cut(s)	886
BsiSI	CCGG	3 cut(s)	805, 1293, 1919
BslFI	GGGAC	1 cut(s)	949
BslI	CCNNNNNNNGG	4 cut(s)	214, 1272, 1716, 2042
BsmAI	GTCTC	3 cut(s)	876, 1242, 1774
BsmFI	GGGAC	1 cut(s)	949
BsmI	GAATGC	3 cut(s)	848, 874, 1441
BsnI	GGCC	4 cut(s)	375, 808, 1208, 1276
Bso31I	GGTCTC	2 cut(s)	876, 1774
BsoBI	CYCGRG	1 cut(s)	886
Bsp119I	TTCGAA	1 cut(s)	784
Bsp1286I	GDGCHC	1 cut(s)	561
Bsp143I	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
Bsp1720I	GCTNAGC	1 cut(s)	1782
Bsp19I	CCATGG	1 cut(s)	1266
Bsp68I	TCGCGA	1 cut(s)	817
BspACI	CCGC	2 cut(s)	353, 1802
BspANI	GGCC	4 cut(s)	375, 808, 1208, 1276
BspCNI	CTCAG	4 cut(s)	1066, 1326, 1707, 1795
BspFNI	CGCG	1 cut(s)	817
BspHI	TCATGA	2 cut(s)	1243, 1420
BspLI	GGNNCC	3 cut(s)	1743, 1834, 1849
BspMAI	CTGCAG	1 cut(s)	456
BspPI	GGATC	5 cut(s)	461, 796, 1114, 1827, 1840
BspT104I	TTCGAA	1 cut(s)	784
BspTNI	GGTCTC	2 cut(s)	876, 1774
BsrI	ACTGG	3 cut(s)	147, 898, 1108
BssECI	CCNNGG	4 cut(s)	1266, 1291, 1368, 1664
BssMI	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
BssNI	GRCGYC	1 cut(s)	177
BssT1I	CCWWGG	2 cut(s)	1266, 1664
Bst2UI	CCWGG	3 cut(s)	861, 1185, 1308
Bst4CI	ACNGT	3 cut(s)	401, 439, 1005
Bst6I	CTCTTC	3 cut(s)	874, 1245, 1267
BstACI	GRCGYC	1 cut(s)	177
BstAPI	GCANNNNNTGC	2 cut(s)	1038, 1139
BstBI	TTCGAA	1 cut(s)	784
BstC8I	GCNNGC	6 cut(s)	162, 456, 819, 989, 1043, 1206
BstDEI	CTNAG	5 cut(s)	303, 1074, 1334, 1715, 1782
BstDSI	CCRYGG	1 cut(s)	1266
BstENI	CCTNNNNNAGG	1 cut(s)	1714
BstF5I	GGATG	9 cut(s)	361, 497, 856, 994, 1118, 1471, 1535, 1580, 1725
BstFNI	CGCG	1 cut(s)	817
BstH2I	RGCGCY	1 cut(s)	255
BstHHI	GCGC	1 cut(s)	254
BstKTI	GATC	6 cut(s)	469, 736, 804, 1109, 1340, 1835
BstMAI	GTCTC	3 cut(s)	876, 1242, 1774
BstMBI	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
BstMWI	GCNNNNNNNGC	9 cut(s)	482, 556, 814, 1029, 1038, 1139, 1205, 1792, 1801
BstNI	CCWGG	3 cut(s)	861, 1185, 1308
BstNSI	RCATGY	3 cut(s)	678, 942, 1384
BstSCI	CCNGG	5 cut(s)	859, 1183, 1291, 1306, 1918
BstSFI	CTRYAG	1 cut(s)	452
BstUI	CGCG	1 cut(s)	817
BstV1I	GCAGC	6 cut(s)	176, 1129, 1132, 1150, 1167, 1323
BstV2I	GAAGAC	1 cut(s)	1422
BstX2I	RGATCY	5 cut(s)	466, 801, 1106, 1337, 1832
BstYI	RGATCY	5 cut(s)	466, 801, 1106, 1337, 1832
Bsu36I	CCTNAGG	1 cut(s)	1074
BsuRI	GGCC	4 cut(s)	375, 808, 1208, 1276
BtgI	CCRYGG	1 cut(s)	1266
BtrI	CACGTC	1 cut(s)	947
BtsCI	GGATG	9 cut(s)	361, 497, 856, 994, 1118, 1471, 1535, 1580, 1725
BtsI	GCAGTG	3 cut(s)	387, 1888, 2048
BtsIMutI	CAGTG	6 cut(s)	387, 397, 1459, 1712, 1888, 2048
BtuMI	TCGCGA	1 cut(s)	817
Cac8I	GCNNGC	6 cut(s)	162, 456, 819, 989, 1043, 1206
CciI	TCATGA	2 cut(s)	1243, 1420
CfoI	GCGC	1 cut(s)	254
Cfr13I	GGNCC	1 cut(s)	374
CseI	GACGC	1 cut(s)	1034
Csp6I	GTAC	1 cut(s)	1447
CviQI	GTAC	1 cut(s)	1447
DdeI	CTNAG	5 cut(s)	303, 1074, 1334, 1715, 1782
DpnI	GATC	6 cut(s)	468, 735, 803, 1108, 1339, 1834
DpnII	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
Eam1104I	CTCTTC	3 cut(s)	874, 1245, 1267
EarI	CTCTTC	3 cut(s)	874, 1245, 1267
EciI	GGCGGA	1 cut(s)	368
Ecl136II	GAGCTC	1 cut(s)	559
Eco130I	CCWWGG	2 cut(s)	1266, 1664
Eco24I	GRGCYC	1 cut(s)	561
Eco31I	GGTCTC	2 cut(s)	876, 1774
Eco47III	AGCGCT	1 cut(s)	253
Eco53kI	GAGCTC	1 cut(s)	559
Eco57I	CTGAAG	1 cut(s)	560
Eco81I	CCTNAGG	1 cut(s)	1074
Eco88I	CYCGRG	1 cut(s)	886
EcoICRI	GAGCTC	1 cut(s)	559
EcoNI	CCTNNNNNAGG	1 cut(s)	1714
EcoRII	CCWGG	3 cut(s)	859, 1183, 1306
EcoT14I	CCWWGG	2 cut(s)	1266, 1664
EcoT38I	GRGCYC	1 cut(s)	561
ErhI	CCWWGG	2 cut(s)	1266, 1664
FaqI	GGGAC	1 cut(s)	949
FblI	GTMKAC	1 cut(s)	943
Fnu4HI	GCNGC	7 cut(s)	165, 1143, 1146, 1164, 1181, 1312, 1802
FokI	GGATG	9 cut(s)	368, 504, 843, 981, 1105, 1458, 1522, 1567, 1732
FriOI	GRGCYC	1 cut(s)	561
Fsp4HI	GCNGC	7 cut(s)	165, 1143, 1146, 1164, 1181, 1312, 1802
FspBI	CTAG	3 cut(s)	1764, 1878, 1892
GlaI	GCGC	1 cut(s)	253
GluI	GCNGC	7 cut(s)	165, 1143, 1146, 1164, 1181, 1312, 1802
GsuI	CTGGAG	1 cut(s)	130
HaeII	RGCGCY	1 cut(s)	255
HaeIII	GGCC	4 cut(s)	375, 808, 1208, 1276
HapII	CCGG	3 cut(s)	805, 1293, 1919
HgaI	GACGC	1 cut(s)	1034
HhaI	GCGC	1 cut(s)	254
Hin1I	GRCGYC	1 cut(s)	177
Hin6I	GCGC	1 cut(s)	252
HinP1I	GCGC	1 cut(s)	252
HincII	GTYRAC	4 cut(s)	319, 1378, 1675, 1960
HindII	GTYRAC	4 cut(s)	319, 1378, 1675, 1960
HpaI	GTTAAC	1 cut(s)	1378
HpaII	CCGG	3 cut(s)	805, 1293, 1919
HphI	GGTGA	6 cut(s)	606, 875, 1206, 1719, 1995, 2005
Hpy166II	GTNNAC	7 cut(s)	319, 361, 614, 944, 1378, 1675, 1960
Hpy188I	TCNGA	7 cut(s)	421, 525, 540, 636, 1335, 1646, 2011
Hpy188III	TCNNGA	9 cut(s)	258, 272, 554, 799, 816, 1244, 1421, 1484, 2036
Hpy8I	GTNNAC	7 cut(s)	319, 361, 614, 944, 1378, 1675, 1960
Hpy99I	CGWCG	1 cut(s)	179
HpyAV	CCTTC	6 cut(s)	404, 436, 535, 1444, 1509, 1990
HpyCH4III	ACNGT	3 cut(s)	401, 439, 1005
HpyCH4IV	ACGT	4 cut(s)	177, 321, 946, 1176
HpyF10VI	GCNNNNNNNGC	9 cut(s)	482, 556, 814, 1029, 1038, 1139, 1205, 1792, 1801
HpyF3I	CTNAG	5 cut(s)	303, 1074, 1334, 1715, 1782
HpySE526I	ACGT	4 cut(s)	177, 321, 946, 1176
Hsp92I	GRCGYC	1 cut(s)	177
HspAI	GCGC	1 cut(s)	252
KspAI	GTTAAC	1 cut(s)	1378
Kzo9I	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
LmnI	GCTCC	2 cut(s)	523, 1741
Lsp1109I	GCAGC	6 cut(s)	176, 1129, 1132, 1150, 1167, 1323
LweI	GCATC	4 cut(s)	472, 865, 1816, 2002
MaeI	CTAG	3 cut(s)	1764, 1878, 1892
MaeII	ACGT	4 cut(s)	177, 321, 946, 1176
MaeIII	GTNAC	4 cut(s)	677, 738, 1212, 1725
MalI	GATC	6 cut(s)	468, 735, 803, 1108, 1339, 1834
MboI	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
MboII	GAAGA	7 cut(s)	440, 693, 891, 1232, 1254, 1332, 1422
MflI	RGATCY	5 cut(s)	466, 801, 1106, 1337, 1832
MhlI	GDGCHC	1 cut(s)	561
MluCI	AATT	6 cut(s)	71, 526, 643, 1390, 1934, 2071
MlyI	GAGTC	7 cut(s)	277, 625, 1006, 1011, 1253, 1477, 1883
MmeI	TCCRAC	1 cut(s)	659
MroXI	GAANNNNTTC	1 cut(s)	790
MseI	TTAA	8 cut(s)	189, 219, 290, 590, 1347, 1377, 1928, 1937
MslI	CAYNNNNRTG	2 cut(s)	861, 1157
MspA1I	CMGCKG	1 cut(s)	167
MspI	CCGG	3 cut(s)	805, 1293, 1919
MspR9I	CCNGG	5 cut(s)	861, 1185, 1293, 1308, 1920
Mva1269I	GAATGC	3 cut(s)	848, 874, 1441
MvaI	CCWGG	3 cut(s)	861, 1185, 1308
MvnI	CGCG	1 cut(s)	817
MwoI	GCNNNNNNNGC	9 cut(s)	482, 556, 814, 1029, 1038, 1139, 1205, 1792, 1801
NciI	CCSGG	2 cut(s)	1293, 1920
NcoI	CCATGG	1 cut(s)	1266
NdeII	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
NlaIV	GGNNCC	3 cut(s)	1743, 1834, 1849
NmeAIII	GCCGAG	1 cut(s)	351
NmuCI	GTSAC	3 cut(s)	738, 1212, 1725
NruI	TCGCGA	1 cut(s)	817
NspI	RCATGY	3 cut(s)	678, 942, 1384
NspV	TTCGAA	1 cut(s)	784
PagI	TCATGA	2 cut(s)	1243, 1420
PciI	ACATGT	3 cut(s)	674, 938, 1380
PctI	GAATGC	3 cut(s)	848, 874, 1441
PdmI	GAANNNNTTC	1 cut(s)	790
PfeI	GAWTC	9 cut(s)	47, 65, 100, 334, 668, 1681, 1981, 2000, 2039
PflMI	CCANNNNNTGG	1 cut(s)	1272
PkrI	GCNGC	7 cut(s)	166, 1144, 1147, 1165, 1182, 1313, 1803
PleI	GAGTC	7 cut(s)	277, 625, 1006, 1011, 1253, 1476, 1882
PpsI	GAGTC	7 cut(s)	277, 625, 1006, 1011, 1253, 1476, 1882
PscI	ACATGT	3 cut(s)	674, 938, 1380
Psp124BI	GAGCTC	1 cut(s)	561
Psp6I	CCWGG	3 cut(s)	859, 1183, 1306
PspGI	CCWGG	3 cut(s)	859, 1183, 1306
PspN4I	GGNNCC	3 cut(s)	1743, 1834, 1849
PspPI	GGNCC	1 cut(s)	374
PstI	CTGCAG	1 cut(s)	456
PsuI	RGATCY	5 cut(s)	466, 801, 1106, 1337, 1832
PvuII	CAGCTG	1 cut(s)	167
RruI	TCGCGA	1 cut(s)	817
RsaI	GTAC	1 cut(s)	1448
RsaNI	GTAC	1 cut(s)	1447
RseI	CAYNNNNRTG	2 cut(s)	861, 1157
SacI	GAGCTC	1 cut(s)	561
SaqAI	TTAA	8 cut(s)	189, 219, 290, 590, 1347, 1377, 1928, 1937
SatI	GCNGC	7 cut(s)	165, 1143, 1146, 1164, 1181, 1312, 1802
Sau3AI	GATC	6 cut(s)	466, 733, 801, 1106, 1337, 1832
Sau96I	GGNCC	1 cut(s)	374
SchI	GAGTC	7 cut(s)	277, 625, 1006, 1011, 1253, 1477, 1883
ScrFI	CCNGG	5 cut(s)	861, 1185, 1293, 1308, 1920
SduI	GDGCHC	1 cut(s)	561
SfaNI	GCATC	4 cut(s)	472, 865, 1816, 2002
SfcI	CTRYAG	1 cut(s)	452
SfuI	TTCGAA	1 cut(s)	784
SmiMI	CAYNNNNRTG	2 cut(s)	861, 1157
SmlI	CTYRAG	2 cut(s)	155, 272
SmoI	CTYRAG	2 cut(s)	155, 272
Sse9I	AATT	6 cut(s)	71, 526, 643, 1390, 1934, 2071
SsiI	CCGC	2 cut(s)	353, 1802
SspI	AATATT	1 cut(s)	1318
SspMI	CTAG	3 cut(s)	1764, 1878, 1892
SstI	GAGCTC	1 cut(s)	561
StyD4I	CCNGG	5 cut(s)	859, 1183, 1291, 1306, 1918
StyI	CCWWGG	2 cut(s)	1266, 1664
TaaI	ACNGT	3 cut(s)	401, 439, 1005
TaiI	ACGT	4 cut(s)	180, 324, 949, 1179
TaqI	TCGA	7 cut(s)	784, 1015, 1190, 1257, 1543, 1732, 2061
TasI	AATT	6 cut(s)	71, 526, 643, 1390, 1934, 2071
TauI	GCSGC	1 cut(s)	1804
TfiI	GAWTC	9 cut(s)	47, 65, 100, 334, 668, 1681, 1981, 2000, 2039
Tru1I	TTAA	8 cut(s)	189, 219, 290, 590, 1347, 1377, 1928, 1937
Tru9I	TTAA	8 cut(s)	189, 219, 290, 590, 1347, 1377, 1928, 1937
TscAI	CASTG	6 cut(s)	394, 404, 1459, 1719, 1888, 2055
TseFI	GTSAC	3 cut(s)	738, 1212, 1725
TseI	GCWGC	6 cut(s)	164, 1142, 1145, 1163, 1180, 1311
Tsp45I	GTSAC	3 cut(s)	738, 1212, 1725
TspDTI	ATGAA	6 cut(s)	783, 983, 1141, 1232, 1409, 1965
TspGWI	ACGGA	2 cut(s)	276, 504
TspRI	CASTG	6 cut(s)	394, 404, 1459, 1719, 1888, 2055
Van91I	CCANNNNNTGG	1 cut(s)	1272
XagI	CCTNNNNNAGG	1 cut(s)	1714
XapI	RAATTY	1 cut(s)	71
XceI	RCATGY	3 cut(s)	678, 942, 1384
XmiI	GTMKAC	1 cut(s)	943
XmnI	GAANNNNTTC	1 cut(s)	790
XspI	CTAG	3 cut(s)	1764, 1878, 1892
ZraI	GACGTC	1 cut(s)	178

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.