pycom12g06010

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003620
Physical Location & Seq
Reverse (-)
6020522 .. 6021940
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g06010.1

Sequence Viewer

Length: 1419 bp
ATGTATTCATTCAAGGTGAGGCCTTGTTCACGTGCATACTCTCATGATTGGACTGAGTGCCCTTTTGTCCATCCAGGGGAAAATGCACGAAGAAGGGATCCAAGGAAGTTTCATTATAGCTGTGTTCCGTGTCCTGATTTCAGAAAGGGGGCTTGCAGACGTGGAGATATGTGTGAATATGCTCATGGGGTTTTTGAGTGCTGGCTACACCCAGCTCAGTATCGAACCCGACTTTGCAAGGATGGTACAACCTGTGCAAGGAGAGTCTGCTTTTTTGCCCACACCACAGAGGAACTCAGGCCATTATACGTTTCTACTGGTTCTGCTGTTCCTTCTCCTCGTTCGAGCACCTCTGGTGCTTCGGCCATGGATTTTGCTGCTGCAATGAGCCTCTTGCCTGGTTCCCCATCATCGGTCAATGTCATGTCTCCTTCACCATTCACTCCACCCATGTCTCCATCTGCCAATGGCATGTCACACTCATCTCTGGGTTGGCCCCAACCTAATGTCCCGGCCTTGCATCTGCCAGGTAGCAATCTTCAGTCTAGTCGCTTGAGATCTTCTCTTTGTGCAAGAGATATGCCAGCGGATGATTTTGATTTACTGCCAGAGTTTGATATGCAGCAACAGCAGCTGCAGCAACAGCAGCTTCTAAATGAATTATCTTGCCTATCCCAACCATCTCTGAGTAATAATTCACTGAACCGTTCTGGTCGAAGGACAACCCTAACCCCCTCTAATCTTGATGATATCTTCTCTGCTGAGAGCTTATCACCCCGGTACTCTGATCGATCATTGCAATCGGGTGTTTTCTCCCCAACTCACAAATCAGCTGTTCTCAATCAATTTCAACAGCAGCAGAGCATACTTTCACCGATCGATATTAACTTTTCTCCAAAAACTGTTGATCATGCTCTATTGCAGGCCTCTTATGGGGGCCCATCCTCTGGGAGGATGTCTCCACGGAATGTGGAACCTATCTCACCAATGGGCTCTCGGGTTTCAATGCTAGCTCAAAGAGAGAAGCAACAACAGTTTCGTAGCCTTAGTTCACGGGAACTCGGCTCCAGTTCTGCATCCATTGTTGGTTCCTCTCCAAATTCTTGGTCAAAATGGGGATCCTCCAATGGAAAACCAGATTGGGCAGTTTCTACAGATGAATTGGGTAAGCTCCGCAGATCATCTTCTTTTGAGCTTGGGAACAATGGAGAGGAGCCGGATCTGTCATGGGTTCAGTCACTTGTTAAAGAATCTCCTACCGACATCAAAGAGAAGCAAACACCGAGCTCAGCTGTTACGGCCACGGGTTCCTCCAACAACGGTCCAAGTGCAAACTCCCAAAGGGAGGCGGTCGATCATGCAGTGTTGGGAGCATGGATTGACCAAATGCATCTTGATCTCGTGGCTCAGCAGAACTGA

Protein Analysis

473

Amino Acids

51.74

Weight (kDa)

8.24

Isoelectric Point (pI)

76.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH_AtC3H23 PF25512 1 - 31 3.7e-19 AtC3H23-like, CCCH zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 947
AciI CCGC 3 cut(s) 587, 1174, 1349
AclWI GGATC 5 cut(s) 92, 105, 1113, 1126, 1227
AcoI YGGCCR 2 cut(s) 363, 1299
AcsI RAATTY 1 cut(s) 1099
AcuI CTGAAG 1 cut(s) 524
AcvI CACGTG 1 cut(s) 32
AfaI GTAC 2 cut(s) 247, 782
AfiI CCNNNNNNNGG 7 cut(s) 76, 258, 412, 933, 947, 951, 1345
AgsI TTSAA 3 cut(s) 13, 851, 1005
AjiI CACGTC 1 cut(s) 161
AjnI CCWGG 3 cut(s) 73, 397, 526
AjuI GAANNNNNNNTTGG 2 cut(s) 1123, 1155
Alw21I GWGCWC 2 cut(s) 350, 1289
Alw26I GTCTC 3 cut(s) 432, 459, 963
AlwI GGATC 5 cut(s) 92, 105, 1113, 1126, 1227
AlwNI CAGNNNCTG 1 cut(s) 634
Ama87I CYCGRG 1 cut(s) 996
AoxI GGCC 8 cut(s) 20, 299, 363, 494, 513, 924, 937, 1299
ApaI GGGCCC 1 cut(s) 941
ApeKI GCWGC 8 cut(s) 377, 380, 622, 631, 634, 637, 646, 856
ApoI RAATTY 1 cut(s) 1099
ArsI GACNNNNNNTTYG 2 cut(s) 1091, 1123
AspS9I GGNCC 4 cut(s) 495, 937, 938, 1322
AsuC2I CCSGG 2 cut(s) 512, 778
AsuHPI GGTGA 5 cut(s) 28, 426, 765, 864, 975
AsuNHI GCTAGC 1 cut(s) 1009
AvaI CYCGRG 1 cut(s) 996
AvaII GGWCC 1 cut(s) 1322
BaeGI GKGCMC 2 cut(s) 62, 941
BamHI GGATCC 2 cut(s) 97, 1118
BanII GRGCYC 3 cut(s) 941, 995, 1289
BauI CACGAG 1 cut(s) 1400
BbrPI CACGTG 1 cut(s) 32
Bbv12I GWGCWC 2 cut(s) 350, 1289
BbvI GCAGC 8 cut(s) 364, 367, 621, 634, 643, 649, 658, 868
BccI CCATC 6 cut(s) 78, 236, 415, 466, 688, 949
BceAI ACGGC 1 cut(s) 1314
BciT130I CCWGG 3 cut(s) 75, 399, 528
BclI TGATCA 1 cut(s) 907
BcnI CCSGG 2 cut(s) 512, 778
BcoDI GTCTC 3 cut(s) 432, 459, 963
BfaI CTAG 2 cut(s) 546, 1010
BfmI CTRYAG 2 cut(s) 635, 1152
BglII AGATCT 1 cut(s) 557
BisI GCNGC 8 cut(s) 378, 381, 623, 632, 635, 638, 647, 857
BlpI GCTNAGC 2 cut(s) 1288, 1407
BlsI GCNGC 8 cut(s) 379, 382, 624, 633, 636, 639, 648, 858
Bme1390I CCNGG 5 cut(s) 75, 399, 512, 528, 778
Bme18I GGWCC 1 cut(s) 1322
BmeT110I CYCGRG 1 cut(s) 996
BmgBI CACGTC 1 cut(s) 161
BmgT120I GGNCC 4 cut(s) 495, 937, 938, 1322
BmrFI CCNGG 5 cut(s) 75, 399, 512, 528, 778
BmsI GCATC 3 cut(s) 529, 1085, 1399
BmtI GCTAGC 1 cut(s) 1013
BplI GAGNNNNNCTC 4 cut(s) 547, 579, 943, 975
BpmI CTGGAG 1 cut(s) 1051
Bpu1102I GCTNAGC 2 cut(s) 1288, 1407
BpuEI CTTGAG 1 cut(s) 574
BpuMI CCSGG 2 cut(s) 512, 778
Bsa29I ATCGAT 2 cut(s) 790, 879
BsaAI YACGTR 1 cut(s) 32
BsaJI CCNNGG 6 cut(s) 74, 101, 366, 776, 962, 1302
Bsc4I CCNNNNNNNGG 7 cut(s) 76, 258, 412, 933, 947, 951, 1345
Bse1I ACTGG 2 cut(s) 322, 1068
Bse3DI GCAATG 2 cut(s) 390, 794
BseBI CCWGG 3 cut(s) 75, 399, 528
BseCI ATCGAT 2 cut(s) 790, 879
BseDI CCNNGG 6 cut(s) 74, 101, 366, 776, 962, 1302
BseGI GGATG 6 cut(s) 70, 247, 595, 941, 960, 1076
BseLI CCNNNNNNNGG 7 cut(s) 76, 258, 412, 933, 947, 951, 1345
BseMI GCAATG 2 cut(s) 390, 794
BseMII CTCAG 6 cut(s) 45, 230, 310, 677, 753, 1302
BseNI ACTGG 2 cut(s) 322, 1068
BseRI GAGGAG 2 cut(s) 327, 1226
BseSI GKGCMC 2 cut(s) 62, 941
BseXI GCAGC 8 cut(s) 364, 367, 621, 634, 643, 649, 658, 868
BseYI CCCAGC 1 cut(s) 211
Bsh1285I CGRYCG 2 cut(s) 879, 1353
BshFI GGCC 8 cut(s) 22, 301, 365, 496, 515, 926, 939, 1301
BshVI ATCGAT 2 cut(s) 790, 879
BsiEI CGRYCG 2 cut(s) 879, 1353
BsiHKAI GWGCWC 2 cut(s) 350, 1289
BsiHKCI CYCGRG 1 cut(s) 996
BsiSI CCGG 3 cut(s) 512, 778, 1217
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 7 cut(s) 76, 258, 412, 933, 947, 951, 1345
BsmAI GTCTC 3 cut(s) 432, 459, 963
BsmFI GGGAC 1 cut(s) 494
BsnI GGCC 8 cut(s) 22, 301, 365, 496, 515, 926, 939, 1301
BsoBI CYCGRG 1 cut(s) 996
Bsp120I GGGCCC 1 cut(s) 937
Bsp1286I GDGCHC 5 cut(s) 62, 350, 941, 995, 1289
Bsp1720I GCTNAGC 2 cut(s) 1288, 1407
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 3 cut(s) 587, 1174, 1349
BspANI GGCC 8 cut(s) 22, 301, 365, 496, 515, 926, 939, 1301
BspCNI CTCAG 6 cut(s) 46, 229, 309, 678, 754, 1301
BspDI ATCGAT 2 cut(s) 790, 879
BspHI TCATGA 1 cut(s) 43
BspMAI CTGCAG 1 cut(s) 639
BspOI GCTAGC 1 cut(s) 1013
BspPI GGATC 5 cut(s) 92, 105, 1113, 1126, 1227
BsrDI GCAATG 2 cut(s) 390, 794
BsrI ACTGG 2 cut(s) 322, 1068
BssECI CCNNGG 6 cut(s) 74, 101, 366, 776, 962, 1302
BssSI CACGAG 1 cut(s) 1400
BssT1I CCWWGG 2 cut(s) 101, 366
Bst2BI CACGAG 1 cut(s) 1400
Bst2UI CCWGG 3 cut(s) 75, 399, 528
Bst4CI ACNGT 4 cut(s) 707, 904, 1035, 1322
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 5 cut(s) 154, 203, 585, 924, 1011
BstDEI CTNAG 8 cut(s) 54, 216, 296, 686, 762, 1046, 1288, 1407
BstDSI CCRYGG 3 cut(s) 366, 962, 1302
BstENI CCTNNNNNAGG 2 cut(s) 256, 949
BstF5I GGATG 6 cut(s) 70, 247, 595, 941, 960, 1076
BstMAI GTCTC 3 cut(s) 432, 459, 963
BstMCI CGRYCG 2 cut(s) 879, 1353
BstMWI GCNNNNNNNGC 6 cut(s) 628, 631, 637, 643, 646, 1298
BstNI CCWGG 3 cut(s) 75, 399, 528
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 5 cut(s) 73, 397, 510, 526, 776
BstSFI CTRYAG 2 cut(s) 635, 1152
BstSLI GKGCMC 2 cut(s) 62, 941
BstV1I GCAGC 8 cut(s) 364, 367, 621, 634, 643, 649, 658, 868
BstX2I RGATCY 4 cut(s) 97, 557, 1118, 1219
BstXI CCANNNNNNTGG 1 cut(s) 1104
BstYI RGATCY 4 cut(s) 97, 557, 1118, 1219
Bsu15I ATCGAT 2 cut(s) 790, 879
BsuRI GGCC 8 cut(s) 22, 301, 365, 496, 515, 926, 939, 1301
BsuTUI ATCGAT 2 cut(s) 790, 879
BtgI CCRYGG 3 cut(s) 366, 962, 1302
BtrI CACGTC 1 cut(s) 161
BtsCI GGATG 6 cut(s) 70, 247, 595, 941, 960, 1076
BtsI GCAGTG 1 cut(s) 1368
BtsIMutI CAGTG 2 cut(s) 698, 1368
Cac8I GCNNGC 5 cut(s) 154, 203, 585, 924, 1011
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 1 cut(s) 43
Cfr13I GGNCC 4 cut(s) 495, 937, 938, 1322
ClaI ATCGAT 2 cut(s) 790, 879
Csp6I GTAC 2 cut(s) 246, 781
CviQI GTAC 2 cut(s) 246, 781
DdeI CTNAG 8 cut(s) 54, 216, 296, 686, 762, 1046, 1288, 1407
EaeI YGGCCR 2 cut(s) 363, 1299
Ecl136II GAGCTC 1 cut(s) 1287
Eco130I CCWWGG 2 cut(s) 101, 366
Eco147I AGGCCT 2 cut(s) 22, 926
Eco24I GRGCYC 3 cut(s) 941, 995, 1289
Eco32I GATATC 1 cut(s) 751
Eco47I GGWCC 1 cut(s) 1322
Eco53kI GAGCTC 1 cut(s) 1287
Eco57I CTGAAG 1 cut(s) 524
Eco72I CACGTG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 996
EcoICRI GAGCTC 1 cut(s) 1287
EcoNI CCTNNNNNAGG 2 cut(s) 256, 949
EcoO109I RGGNCCY 1 cut(s) 937
EcoRII CCWGG 3 cut(s) 73, 397, 526
EcoRV GATATC 1 cut(s) 751
EcoT14I CCWWGG 2 cut(s) 101, 366
EcoT22I ATGCAT 1 cut(s) 1392
EcoT38I GRGCYC 3 cut(s) 941, 995, 1289
ErhI CCWWGG 2 cut(s) 101, 366
FaqI GGGAC 1 cut(s) 494
FbaI TGATCA 1 cut(s) 907
Fnu4HI GCNGC 8 cut(s) 378, 381, 623, 632, 635, 638, 647, 857
FokI GGATG 6 cut(s) 57, 254, 602, 928, 967, 1063
FriOI GRGCYC 3 cut(s) 941, 995, 1289
Fsp4HI GCNGC 8 cut(s) 378, 381, 623, 632, 635, 638, 647, 857
FspBI CTAG 2 cut(s) 546, 1010
GluI GCNGC 8 cut(s) 378, 381, 623, 632, 635, 638, 647, 857
GsaI CCCAGC 1 cut(s) 215
GsuI CTGGAG 1 cut(s) 1051
HaeIII GGCC 8 cut(s) 22, 301, 365, 496, 515, 926, 939, 1301
HapII CCGG 3 cut(s) 512, 778, 1217
HinfI GANTC 2 cut(s) 264, 1250
HpaII CCGG 3 cut(s) 512, 778, 1217
HphI GGTGA 5 cut(s) 28, 426, 765, 864, 975
Hpy166II GTNNAC 2 cut(s) 29, 1052
Hpy188I TCNGA 3 cut(s) 143, 687, 787
Hpy188III TCNNGA 4 cut(s) 44, 134, 743, 1394
Hpy8I GTNNAC 2 cut(s) 29, 1052
HpyAV CCTTC 4 cut(s) 87, 342, 441, 711
HpyCH4III ACNGT 4 cut(s) 707, 904, 1035, 1322
HpyCH4IV ACGT 3 cut(s) 31, 160, 309
HpyF10VI GCNNNNNNNGC 6 cut(s) 628, 631, 637, 643, 646, 1298
HpyF3I CTNAG 8 cut(s) 54, 216, 296, 686, 762, 1046, 1288, 1407
HpySE526I ACGT 3 cut(s) 31, 160, 309
Ksp22I TGATCA 1 cut(s) 907
LmnI GCTCC 4 cut(s) 1070, 1176, 1213, 1370
Lsp1109I GCAGC 8 cut(s) 364, 367, 621, 634, 643, 649, 658, 868
LweI GCATC 3 cut(s) 529, 1085, 1399
MaeI CTAG 2 cut(s) 546, 1010
MaeII ACGT 3 cut(s) 31, 160, 309
MaeIII GTNAC 3 cut(s) 474, 1236, 1294
MboII GAAGA 5 cut(s) 102, 530, 552, 745, 1176
MflI RGATCY 4 cut(s) 97, 557, 1118, 1219
MhlI GDGCHC 5 cut(s) 62, 350, 941, 995, 1289
MluCI AATT 5 cut(s) 659, 694, 845, 1099, 1160
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 1338
Mph1103I ATGCAT 1 cut(s) 1392
MseI TTAA 2 cut(s) 885, 1245
MspA1I CMGCKG 4 cut(s) 587, 634, 833, 1292
MspI CCGG 3 cut(s) 512, 778, 1217
MspR9I CCNGG 5 cut(s) 75, 399, 512, 528, 778
MvaI CCWGG 3 cut(s) 75, 399, 528
MwoI GCNNNNNNNGC 6 cut(s) 628, 631, 637, 643, 646, 1298
NciI CCSGG 2 cut(s) 512, 778
NcoI CCATGG 1 cut(s) 366
NheI GCTAGC 1 cut(s) 1009
NmeAIII GCCGAG 1 cut(s) 1041
NmuCI GTSAC 2 cut(s) 474, 1236
NsiI ATGCAT 1 cut(s) 1392
NspI RCATGY 1 cut(s) 475
PagI TCATGA 1 cut(s) 43
PceI AGGCCT 2 cut(s) 22, 926
PfeI GAWTC 1 cut(s) 1250
PflMI CCANNNNNTGG 1 cut(s) 947
PkrI GCNGC 8 cut(s) 379, 382, 624, 633, 636, 639, 648, 858
Ple19I CGATCG 1 cut(s) 879
PleI GAGTC 1 cut(s) 272
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
PpsI GAGTC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 1289
Psp6I CCWGG 3 cut(s) 73, 397, 526
PspCI CACGTG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 3 cut(s) 73, 397, 526
PspOMI GGGCCC 1 cut(s) 937
PspPI GGNCC 4 cut(s) 495, 937, 938, 1322
PsrI GAACNNNNNNTAC 2 cut(s) 1033, 1065
PstI CTGCAG 1 cut(s) 639
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 4 cut(s) 97, 557, 1118, 1219
PvuI CGATCG 1 cut(s) 879
PvuII CAGCTG 3 cut(s) 634, 833, 1292
RsaI GTAC 2 cut(s) 247, 782
RsaNI GTAC 2 cut(s) 246, 781
SacI GAGCTC 1 cut(s) 1289
SaqAI TTAA 2 cut(s) 885, 1245
SatI GCNGC 8 cut(s) 378, 381, 623, 632, 635, 638, 647, 857
Sau96I GGNCC 4 cut(s) 495, 937, 938, 1322
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 5 cut(s) 75, 399, 512, 528, 778
SduI GDGCHC 5 cut(s) 62, 350, 941, 995, 1289
SfaNI GCATC 3 cut(s) 529, 1085, 1399
SfcI CTRYAG 2 cut(s) 635, 1152
SinI GGWCC 1 cut(s) 1322
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
Sse9I AATT 5 cut(s) 659, 694, 845, 1099, 1160
SseBI AGGCCT 2 cut(s) 22, 926
SsiI CCGC 3 cut(s) 587, 1174, 1349
SspMI CTAG 2 cut(s) 546, 1010
SstI GAGCTC 1 cut(s) 1289
StuI AGGCCT 2 cut(s) 22, 926
StyD4I CCNGG 5 cut(s) 73, 397, 510, 526, 776
StyI CCWWGG 2 cut(s) 101, 366
TaaI ACNGT 4 cut(s) 707, 904, 1035, 1322
TaiI ACGT 3 cut(s) 34, 163, 312
TaqI TCGA 6 cut(s) 223, 344, 715, 790, 879, 1353
TaqII GACCGA 1 cut(s) 403
TasI AATT 5 cut(s) 659, 694, 845, 1099, 1160
TfiI GAWTC 1 cut(s) 1250
Tru1I TTAA 2 cut(s) 885, 1245
Tru9I TTAA 2 cut(s) 885, 1245
TscAI CASTG 2 cut(s) 705, 1368
TseFI GTSAC 2 cut(s) 474, 1236
TseI GCWGC 8 cut(s) 377, 380, 622, 631, 634, 637, 646, 856
Tsp45I GTSAC 2 cut(s) 474, 1236
TspDTI ATGAA 3 cut(s) 101, 672, 1173
TspGWI ACGGA 2 cut(s) 117, 979
TspRI CASTG 2 cut(s) 705, 1368
Van91I CCANNNNNTGG 1 cut(s) 947
VpaK11BI GGWCC 1 cut(s) 1322
XagI CCTNNNNNAGG 2 cut(s) 256, 949
XapI RAATTY 1 cut(s) 1099
XceI RCATGY 1 cut(s) 475
XspI CTAG 2 cut(s) 546, 1010
Zsp2I ATGCAT 1 cut(s) 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.