MD03G1241200.v1.1

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005139
Physical Location & Seq
Forward (+)
32616550 .. 32626671
10122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1241200.v1.1.491

Sequence Viewer

Length: 3975 bp
ATGTTTCTCAGCAAAGAAATTGACGATTTGCATGATGATGGGTTTGAAGGATCGAAAAATGAGCACTGTATATTCACAGAGGTTTTCTTCGGTCAAGACATTGGTCGTACTAGTAATAGATGTCTTGTTACTGGAATGATCAACTTTGAATGTGAATCAAGTAAGAACACTGATGGAGCACCATCTCCAAATAGTGAAAACTCAGTTGTGACGAGTCACTCCTCAAAAAATACCTGTTTAGAGGAGTTTTATAATGCAACTGAAGATTTTAGAGAAACCTCTGCACCAGGGTTTTCCTTGGACAGATCTGCTATGTTAGATAGGAATGGTGATGATGTGACTGTTAATAGAATGAAGTTTTCAGTCGATGAGCTTCCTAATACCAAACCTGGTTTAGGAAAGGTCACGTCCGGTCCAGCTTCAAACTCTGTCTCTGAAACAGTGACATTTCGTTTGGTTGAATCTTCTAGCCAGGGAGTCACGTCTAGTTGTTATCTGTTTAAGCAACATGCAGAACTTAACAAACCGGGCGTAGCAGGTGACCCAGATGTTTCAAAGTGCAGATTGAGATACTCAAATGGGGATGATAGAAAGGAAGTCTGCCTAAGTAAAGCTATTGCTTCGCCTGCTTCACATGAGAACTTCTTGACCAGGCTTCTGGTTGCAAGTCCATTAGCCACTGTTATCGATAAATCCAGAACTCCTCTACATTCTGAGGGAAAGCCGAACGGATTTGAATCTTCTGTTTTGGATGTCTCCAATGTTGCCTTGAAGACAGAGAATAGTAAGGACCCTCGTCCACTTCTCCGATATCATGTTGCTCGCTTGCTTGAAGCTGCTGGATGGCATATTGAGAGGCGAAAAAGACCCAGTAGAAATTATATGGAGACTGTTTATATGACACCTAAGGGAAGGTTAATTTGGGAACTCCCCAAAGCTTGGCGGTTATGTGGAGAACTTCTGTTAGCAGATGGGTACAGTATGCTGCAGGAAGATGATACGAAGGAGTGGGCCAACATCAGTCAGTTCTGGTCAGATCTGTCTGGTGTTATAGTAAATATTGGGAAAGAGATTCATCATCCACAACCTGCTACTGCATTGGCTTACTGGTGGAGGCTGTTAGATCCTTTTGTGGTTGTCGTGTTTATTGAGAGAAAGATTGGTTGTCTAAGAAAGGGAGAGATAGTTAAAGCAACTCAAAGTGTAGCTGATTCGAACCGTAAGACTAATTCTGCTTTGGCCCTGACTAGTGCCAATAACATAGAAAGTCATTTTACTAAGGAAGATGTGTCAGCTCCTCTCCATGACTCCACACTCGCTAGGAGGGTTGGATTAGCAGTTTCTAAGCGAGATTACAATGCCTGTGGGAAATTTGGTAATGAAAGTTCCTCAAAACATTGCGGACAGACAAGTAATGAGGAAGTGAAATTTCTTACGGGACTATCTATTTATTCGGCTAATGCAGAAAGTCATTGCTTGGGGAATGCTGGTAATAGAGTTGAAAATGGGTTCTCTGGGAAAATAACTTGTCCTGATTCCTTACCAGTTTGTGGGTCTGGTGGCATTTGTATTCAGTCAGCTACTCATCGTGAAGAACCTATTACTTCCAGAAATTGTGACAATGTGCATGGAGGTTCTGAAGCTGTCTCGCCTCACCAAGATGGCAATGCAAATTCCCCTGGCTCTAATAAACAGAGTTCTGGACTTGATTTAGAAACGCCTAAGGAAGTTATGGAAGACACCTCAGTGGATTCTTCTGAGGAAAAAGATGAATCAGAGGGAGGCAAGGTTGATGATAAGCTGGAAAGCGCTCAGAAGGGATCATTAGATAACCAAGGAAACTACACAATTGATTTTCTGAAAAGAAAAACACGTAGGAAGTCTAAAAAGATATCTGAAATTGAACCAAGCTTGTTATACCGAAGTAGCCTTTTAGATTTTACTTCAAGTGAGAATGCTGATTCGCTGTGTATTGATGCTAATGGTACCCAGTCAAAGTCGACAGAGGTCCAGGATGAATTTACATGCAATAAAAGATGCAAAGGAAGTCGCAAGATGTCCTTACCTACCGACTCTTGCCAGCAACAGATTGGGAGAAAAAGTTCAAAGTTGATGAAGATTTCTCGTGAATGTGATGATGTCCAGACTGGAAGAAGTAAATCATTTAAATGCCAAATTGAAGATGATGACTTGTTGGTTTCAGCTATTATAAAAAATAAAGATTTTAGGACAATGCATGCTCAATATTCTTCTAGAAAGAAAGCCTACAAATCAAGAGCTCATAAGAAAGGTAAAACTAAAAGCCAAAAGAGTCGTTGCAAGTTGCTTCCAAGAAGCCTGGGCAGTGGGGGAAAGCACTTTAAGGATGGAAAGTGGCATTCTGTAGGAGTGAAAACAGTTTTGTCTTGGTTAATTGATGCTGGTGTCATATCTCCAGATGATGTGATTCAGTATCGGAATCCTAAGGATGGTGGTGTCCTTATAGATGGTCTGGTTACCAGGGATGGCATTTTTTGCAAGTGTTGCAGTAAAGTAGTTTCAGTTTCTGAGTTCAAGAGTCATGGTGGATTCAAACAGAATCGTCCATGTTTGAATCTTTTCATGGAATCTGGTCAGTCCTTCACCTTATGCCAGCTTCAGGCTTGGTCAGCTGAATACAAGACCAGGAAAAAGAGCACCCAAGTCGTGCGAGATGATCAAAATGATCAAAATGATGATTCATGTGGACTTTGTGGTGATGGTGGCGAGTTGATATGCTGTGATAATTGCCCCTCAACTTTTCATCAGGCTTGTTTGTCTCTGCAGGAGCTCCCTGAAGGCAACTGGTATTGCTCGAATTGCACCTGTTGGATATGTGGTGATTTTGTCAATGATAAGGGGGCTTCGAGTCGTGATGGATTTAAGTGTTCACAGTGTGAGCATAAATATCATGCGGCATGCCTGAAAGATAAATGTACCTACGGAGCCATACCGGATACTTGTTTCTGTAATAGAAGCTGTCAGGAGGCTTATTCTGGTCTTCAATCTCGCGTTGGCTGCATTAATCATTTTGCTGATGGATTTTCATGGACACTACTTAGATGCATTCATGATGACCAAAAGGGTCATTCTGCTCAACGGTTTGCTCTTAAGGCGGAATGCAATACAAAACTAGCTGTTTCTCTTTCAATAATGGAGGAATGTTTTTTGTCAATGGTTGATCCAAGAACAGGCATAGACATGATACCCCATGTTTTGTACAATTGGGGGTCAGAGTTTGCACGTTTGAATTTTCAAGGCTTTTACACTGCGGTCTTGGAGAAAGATGATGCATTGGTATCTGTAGCATCCATCAGGGTGCATGGAACAACAGTTGCAGAGATGCCGCTCATTGCAACTTGCAGTAATTTCCGTCGCCAGGGAATGTGCCGACGCCTTCTGACTGCGATTGAAGAGATGCTAATATCTTTTAAGGTTGAAAAGCTTGTCGTAGCTGCCATTCCCGATTTGGTAGAGACGTGGACACAGGGTTTTGGCTTTGTACCCGTAGAAGGCGACGAGAAGCGTAGTTTGAACAAGATCAACTTGATGGTCTTTCCTGGAACAATGCTGCTCCAAAAACCCTTGTACGAAAATCAAAAAGCACGTAGGCATTCAGCAGAAATGGGAACCAACAGAGCTGTTGCTGATGCGGAGTTAGAAGTCGGTTCTGAAGCTGAATTCAACGATGCTATCCAGCCATCGGAGGGAACCGGTTGTCAGATCGAAGAAGGCTGGGAATCTGAACTTGGTACTAAGGAAGCAGAAGTCATGGGCAGCAAGAATGTGCAACGTGAAGCCGAAGGAAGCACTTTGCAGGGCGAATTTTCAAAGCTGTCTAGTGAAGAAGTGGCAGATCTAACATTAGGAAATAGCGACGGGCGTGAAACCGTGACTAATGTTGAATCCTTGAAAATGTATGATGAAATACAGCAGCAGCTTCCTTTGGAAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1325

Amino Acids

146.3

Weight (kDa)

5.95

Isoelectric Point (pI)

44.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 830 - 880 1.4e-17 Tify domain binding domain
PHD-1st_NSD PF23011 905 - 947 7.2e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 908 - 947 3.3e-07 PHD-finger
IDM1_C PF23209 1062 - 1201 1.2e-58 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 252, 2210
AarI CACCTGC 1 cut(s) 527
Acc36I ACCTGC 2 cut(s) 527, 1096
Acc65I GGTACC 1 cut(s) 1985
AccB1I GGYRCC 1 cut(s) 1985
AccB7I CCANNNNNTGG 2 cut(s) 939, 1550
AccBSI CCGCTC 1 cut(s) 3367
AccI GTMKAC 1 cut(s) 2000
AccII CGCG 1 cut(s) 3030
AciI CCGC 7 cut(s) 943, 1401, 2933, 3134, 3290, 3365, 3671
AclWI GGATC 4 cut(s) 58, 1118, 1828, 3194
AcsI RAATTY 7 cut(s) 1370, 1427, 1672, 2018, 3268, 3698, 3842
AcuI CTGAAG 5 cut(s) 282, 1659, 2621, 2835, 3711
AcyI GRCGYC 1 cut(s) 3412
AdeI CACNNNGTG 1 cut(s) 2915
AfaI GTAC 8 cut(s) 109, 977, 1987, 2956, 3239, 3522, 3608, 3772
AfeI AGCGCT 1 cut(s) 1810
AflII CTTAAG 1 cut(s) 3128
AflIII ACRYGT 1 cut(s) 1871
AgeI ACCGGT 1 cut(s) 3731
AhlI ACTAGT 2 cut(s) 110, 1247
AjiI CACGTC 3 cut(s) 408, 483, 3498
AjuI GAANNNNNNNTTGG 4 cut(s) 904, 936, 3750, 3782
AleI CACNNNNGTG 1 cut(s) 1745
AloI GAACNNNNNNTCC 2 cut(s) 2086, 2118
Alw21I GWGCWC 5 cut(s) 66, 181, 2281, 2678, 2811
Alw26I GTCTC 6 cut(s) 436, 760, 881, 1651, 2802, 3488
AlwI GGATC 4 cut(s) 58, 1118, 1828, 3194
AlwNI CAGNNNCTG 1 cut(s) 2546
Aor51HI AGCGCT 1 cut(s) 1810
AoxI GGCC 2 cut(s) 1011, 1239
ApeKI GCWGC 8 cut(s) 836, 985, 3036, 3473, 3589, 3795, 3952, 3955
ApoI RAATTY 7 cut(s) 1370, 1427, 1672, 2018, 3268, 3698, 3842
ArsI GACNNNNNNTTYG 2 cut(s) 436, 468
AseI ATTAAT 1 cut(s) 3042
AsiGI ACCGGT 1 cut(s) 3731
Asp700I GAANNNNTTC 2 cut(s) 2101, 2597
Asp718I GGTACC 1 cut(s) 1985
AspLEI GCGC 1 cut(s) 1811
AspS9I GGNCC 5 cut(s) 413, 790, 1011, 1240, 2008
AsuC2I CCSGG 1 cut(s) 528
AsuHPI GGTGA 6 cut(s) 341, 551, 1646, 2614, 2747, 2870
AsuII TTCGAA 1 cut(s) 1214
AvaII GGWCC 3 cut(s) 413, 790, 2008
AxyI CCTNAGG 3 cut(s) 906, 1722, 2463
BanI GGYRCC 1 cut(s) 1985
BanII GRGCYC 2 cut(s) 2281, 2811
BauI CACGAG 1 cut(s) 2124
BbsI GAAGAC 3 cut(s) 779, 1743, 3011
Bbv12I GWGCWC 5 cut(s) 66, 181, 2281, 2678, 2811
BbvI GCAGC 8 cut(s) 823, 972, 3023, 3460, 3576, 3807, 3964, 3967
BcgI CGANNNNNNTGC 2 cut(s) 2665, 2699
BciVI GTATCC 1 cut(s) 2968
BclI TGATCA 3 cut(s) 138, 2695, 2704
BcnI CCSGG 1 cut(s) 528
BcoDI GTCTC 6 cut(s) 436, 760, 881, 1651, 2802, 3488
BcuI ACTAGT 2 cut(s) 110, 1247
BfaI CTAG 8 cut(s) 111, 468, 486, 1248, 1320, 2253, 3152, 3858
BfmI CTRYAG 4 cut(s) 986, 2382, 2801, 3321
BfoI RGCGCY 1 cut(s) 1812
BfrI CTTAAG 1 cut(s) 3128
BfuAI ACCTGC 2 cut(s) 527, 1096
BfuI GTATCC 1 cut(s) 2968
BglII AGATCT 3 cut(s) 305, 1036, 3874
Bme18I GGWCC 3 cut(s) 413, 790, 2008
BmgBI CACGTC 3 cut(s) 408, 483, 3498
BmgT120I GGNCC 5 cut(s) 413, 790, 1011, 1240, 2008
BmiI GGNNCC 5 cut(s) 792, 1987, 2965, 3649, 3730
BmrI ACTGGG 2 cut(s) 864, 1984
BmuI ACTGGG 2 cut(s) 864, 1984
BoxI GACNNNNGTC 3 cut(s) 102, 795, 2006
BpiI GAAGAC 3 cut(s) 779, 1743, 3011
BpmI CTGGAG 1 cut(s) 2418
Bpu14I TTCGAA 1 cut(s) 1214
BpuMI CCSGG 1 cut(s) 528
Bsa29I ATCGAT 1 cut(s) 687
BsaAI YACGTR 2 cut(s) 1874, 3626
BsaBI GATNNNNATC 1 cut(s) 736
BsaHI GRCGYC 1 cut(s) 3412
BsaJI CCNNGG 8 cut(s) 287, 297, 472, 1678, 1834, 2338, 2499, 3397
BsaWI WCCGGW 3 cut(s) 410, 2971, 3731
BsaXI ACNNNNNCTCC 6 cut(s) 1170, 1200, 1773, 1803, 2086, 2116
Bse118I RCCGGY 1 cut(s) 3731
Bse1I ACTGG 7 cut(s) 136, 870, 1112, 1544, 1990, 2152, 2828
Bse21I CCTNAGG 3 cut(s) 906, 1722, 2463
Bse3DI GCAATG 4 cut(s) 1396, 1471, 1672, 3369
Bse8I GATNNNNATC 1 cut(s) 736
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 8 cut(s) 287, 297, 472, 1678, 1834, 2338, 2499, 3397
BseGI GGATG 9 cut(s) 589, 757, 848, 1078, 2020, 2371, 2473, 2509, 3326
BseJI GATNNNNATC 1 cut(s) 736
BseMI GCAATG 4 cut(s) 1396, 1471, 1672, 3369
BseMII CTCAG 7 cut(s) 22, 216, 705, 1749, 1758, 1826, 2538
BseNI ACTGG 7 cut(s) 136, 870, 1112, 1544, 1990, 2152, 2828
BseRI GAGGAG 4 cut(s) 211, 257, 693, 1287
BseXI GCAGC 8 cut(s) 823, 972, 3023, 3460, 3576, 3807, 3964, 3967
BseYI CCCAGC 1 cut(s) 3753
BsgI GTGCAG 2 cut(s) 267, 580
Bsh1236I CGCG 1 cut(s) 3030
BshFI GGCC 2 cut(s) 1013, 1241
BshNI GGYRCC 1 cut(s) 1985
BshTI ACCGGT 1 cut(s) 3731
BshVI ATCGAT 1 cut(s) 687
BsiHKAI GWGCWC 5 cut(s) 66, 181, 2281, 2678, 2811
BsiSI CCGG 4 cut(s) 411, 527, 2972, 3732
BslFI GGGAC 1 cut(s) 1452
BsmAI GTCTC 6 cut(s) 436, 760, 881, 1651, 2802, 3488
BsmBI CGTCTC 1 cut(s) 3488
BsmFI GGGAC 1 cut(s) 1452
BsmI GAATGC 6 cut(s) 1489, 1960, 2377, 3084, 3143, 3631
BsnI GGCC 2 cut(s) 1013, 1241
Bsp119I TTCGAA 1 cut(s) 1214
Bsp1286I GDGCHC 5 cut(s) 66, 181, 2281, 2678, 2811
Bsp1407I TGTACA 1 cut(s) 3237
BspACI CCGC 7 cut(s) 943, 1401, 2933, 3134, 3290, 3365, 3671
BspANI GGCC 2 cut(s) 1013, 1241
BspCNI CTCAG 7 cut(s) 21, 215, 706, 1750, 1757, 1825, 2539
BspDI ATCGAT 1 cut(s) 687
BspFNI CGCG 1 cut(s) 3030
BspHI TCATGA 1 cut(s) 3088
BspLI GGNNCC 5 cut(s) 792, 1987, 2965, 3649, 3730
BspMAI CTGCAG 2 cut(s) 990, 2805
BspMI ACCTGC 2 cut(s) 527, 1096
BspPI GGATC 4 cut(s) 58, 1118, 1828, 3194
BspT104I TTCGAA 1 cut(s) 1214
BspT107I GGYRCC 1 cut(s) 1985
BspTI CTTAAG 1 cut(s) 3128
BsrBI CCGCTC 1 cut(s) 3367
BsrDI GCAATG 4 cut(s) 1396, 1471, 1672, 3369
BsrFI RCCGGY 1 cut(s) 3731
BsrGI TGTACA 1 cut(s) 3237
BsrI ACTGG 7 cut(s) 136, 870, 1112, 1544, 1990, 2152, 2828
BssAI RCCGGY 1 cut(s) 3731
BssECI CCNNGG 8 cut(s) 287, 297, 472, 1678, 1834, 2338, 2499, 3397
BssNI GRCGYC 1 cut(s) 3412
BssSI CACGAG 1 cut(s) 2124
BssT1I CCWWGG 2 cut(s) 297, 1834
Bst2BI CACGAG 1 cut(s) 2124
Bst6I CTCTTC 1 cut(s) 3426
BstACI GRCGYC 1 cut(s) 3412
BstAFI CTTAAG 1 cut(s) 3128
BstAPI GCANNNNNTGC 2 cut(s) 2514, 2523
BstAUI TGTACA 1 cut(s) 3237
BstBAI YACGTR 2 cut(s) 1874, 3626
BstBI TTCGAA 1 cut(s) 1214
BstC8I GCNNGC 7 cut(s) 627, 823, 827, 2081, 2238, 2633, 2938
BstEII GGTNACC 2 cut(s) 539, 2494
BstENI CCTNNNNNAGG 1 cut(s) 393
BstF5I GGATG 9 cut(s) 589, 757, 848, 1078, 2020, 2371, 2473, 2509, 3326
BstFNI CGCG 1 cut(s) 3030
BstH2I RGCGCY 1 cut(s) 1812
BstHHI GCGC 1 cut(s) 1811
BstMAI GTCTC 6 cut(s) 436, 760, 881, 1651, 2802, 3488
BstMWI GCNNNNNNNGC 7 cut(s) 626, 2514, 2523, 2648, 2838, 3036, 3131
BstNSI RCATGY 4 cut(s) 512, 2028, 2240, 2940
BstPAI GACNNNNGTC 3 cut(s) 102, 795, 2006
BstPI GGTNACC 2 cut(s) 539, 2494
BstSFI CTRYAG 4 cut(s) 986, 2382, 2801, 3321
BstUI CGCG 1 cut(s) 3030
BstV1I GCAGC 8 cut(s) 823, 972, 3023, 3460, 3576, 3807, 3964, 3967
BstV2I GAAGAC 3 cut(s) 779, 1743, 3011
BstX2I RGATCY 4 cut(s) 305, 1036, 1123, 3874
BstXI CCANNNNNNTGG 1 cut(s) 658
BstYI RGATCY 4 cut(s) 305, 1036, 1123, 3874
Bsu15I ATCGAT 1 cut(s) 687
Bsu36I CCTNAGG 3 cut(s) 906, 1722, 2463
BsuI GTATCC 1 cut(s) 2968
BsuRI GGCC 2 cut(s) 1013, 1241
BsuTUI ATCGAT 1 cut(s) 687
BtrI CACGTC 3 cut(s) 408, 483, 3498
BtsCI GGATG 9 cut(s) 589, 757, 848, 1078, 2020, 2371, 2473, 2509, 3326
BtsI GCAGTG 2 cut(s) 2350, 3285
BtsIMutI CAGTG 8 cut(s) 64, 168, 447, 678, 1752, 2350, 2918, 3285
BveI ACCTGC 2 cut(s) 527, 1096
Cac8I GCNNGC 7 cut(s) 627, 823, 827, 2081, 2238, 2633, 2938
CaiI CAGNNNCTG 1 cut(s) 2546
CciI TCATGA 1 cut(s) 3088
CfoI GCGC 1 cut(s) 1811
Cfr10I RCCGGY 1 cut(s) 3731
Cfr13I GGNCC 5 cut(s) 413, 790, 1011, 1240, 2008
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 1 cut(s) 3420
CsiI ACCWGGT 1 cut(s) 388
Csp6I GTAC 8 cut(s) 108, 976, 1986, 2955, 3238, 3521, 3607, 3771
CspAI ACCGGT 1 cut(s) 3731
CspCI CAANNNNNGTGG 2 cut(s) 1345, 1380
CviQI GTAC 8 cut(s) 108, 976, 1986, 2955, 3238, 3521, 3607, 3771
DraI TTTAAA 1 cut(s) 2167
DraIII CACNNNGTG 1 cut(s) 2915
Eam1104I CTCTTC 1 cut(s) 3426
EarI CTCTTC 1 cut(s) 3426
EciI GGCGGA 1 cut(s) 3149
Ecl136II GAGCTC 2 cut(s) 2279, 2809
Eco130I CCWWGG 2 cut(s) 297, 1834
Eco24I GRGCYC 2 cut(s) 2281, 2811
Eco32I GATATC 2 cut(s) 812, 1893
Eco47I GGWCC 3 cut(s) 413, 790, 2008
Eco47III AGCGCT 1 cut(s) 1810
Eco53kI GAGCTC 2 cut(s) 2279, 2809
Eco57I CTGAAG 5 cut(s) 282, 1659, 2621, 2835, 3711
Eco81I CCTNAGG 3 cut(s) 906, 1722, 2463
Eco91I GGTNACC 2 cut(s) 539, 2494
EcoICRI GAGCTC 2 cut(s) 2279, 2809
EcoNI CCTNNNNNAGG 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 790
EcoO65I GGTNACC 2 cut(s) 539, 2494
EcoRI GAATTC 1 cut(s) 3698
EcoRV GATATC 2 cut(s) 812, 1893
EcoT14I CCWWGG 2 cut(s) 297, 1834
EcoT22I ATGCAT 3 cut(s) 2238, 3086, 3313
EcoT38I GRGCYC 2 cut(s) 2281, 2811
ErhI CCWWGG 2 cut(s) 297, 1834
Esp3I CGTCTC 1 cut(s) 3488
FalI AAGNNNNNCTT 4 cut(s) 2045, 2077, 3548, 3580
FaqI GGGAC 1 cut(s) 1452
FbaI TGATCA 3 cut(s) 138, 2695, 2704
FblI GTMKAC 1 cut(s) 2000
FokI GGATG 9 cut(s) 596, 764, 855, 1065, 2027, 2378, 2480, 2516, 3313
FriOI GRGCYC 2 cut(s) 2281, 2811
FspBI CTAG 8 cut(s) 111, 468, 486, 1248, 1320, 2253, 3152, 3858
GlaI GCGC 1 cut(s) 1810
GsaI CCCAGC 1 cut(s) 3757
GsuI CTGGAG 1 cut(s) 2418
HaeII RGCGCY 1 cut(s) 1812
HaeIII GGCC 2 cut(s) 1013, 1241
HapII CCGG 4 cut(s) 411, 527, 2972, 3732
HgaI GACGC 1 cut(s) 3420
HhaI GCGC 1 cut(s) 1811
Hin1I GRCGYC 1 cut(s) 3412
Hin6I GCGC 1 cut(s) 1809
HinP1I GCGC 1 cut(s) 1809
HincII GTYRAC 1 cut(s) 2001
HindII GTYRAC 1 cut(s) 2001
HindIII AAGCTT 3 cut(s) 936, 1909, 3461
HpaII CCGG 4 cut(s) 411, 527, 2972, 3732
HphI GGTGA 6 cut(s) 341, 551, 1646, 2614, 2747, 2870
Hpy166II GTNNAC 5 cut(s) 800, 2001, 2726, 2909, 3501
Hpy8I GTNNAC 5 cut(s) 800, 2001, 2726, 2909, 3501
Hpy99I CGWCG 4 cut(s) 3396, 3414, 3539, 3899
HpyCH4IV ACGT 7 cut(s) 407, 482, 1873, 3262, 3497, 3625, 3811
HpyF10VI GCNNNNNNNGC 7 cut(s) 626, 2514, 2523, 2648, 2838, 3036, 3131
HpySE526I ACGT 7 cut(s) 407, 482, 1873, 3262, 3497, 3625, 3811
Hsp92I GRCGYC 1 cut(s) 3412
HspAI GCGC 1 cut(s) 1809
KpnI GGTACC 1 cut(s) 1989
Ksp22I TGATCA 3 cut(s) 138, 2695, 2704
LmnI GCTCC 6 cut(s) 176, 1300, 2806, 2814, 2963, 3597
Lsp1109I GCAGC 8 cut(s) 823, 972, 3023, 3460, 3576, 3807, 3964, 3967
MabI ACCWGGT 1 cut(s) 388
MaeI CTAG 8 cut(s) 111, 468, 486, 1248, 1320, 2253, 3152, 3858
MaeII ACGT 7 cut(s) 407, 482, 1873, 3262, 3497, 3625, 3811
MbiI CCGCTC 1 cut(s) 3367
MfeI CAATTG 2 cut(s) 1848, 3241
MflI RGATCY 4 cut(s) 305, 1036, 1123, 3874
MhlI GDGCHC 5 cut(s) 66, 181, 2281, 2678, 2811
MlyI GAGTC 7 cut(s) 223, 486, 1301, 2066, 2320, 2566, 2896
MmeI TCCRAC 2 cut(s) 1309, 2828
Mph1103I ATGCAT 3 cut(s) 2238, 3086, 3313
MroXI GAANNNNTTC 2 cut(s) 2101, 2597
MslI CAYNNNNRTG 6 cut(s) 36, 1659, 1745, 2167, 3218, 3335
MspA1I CMGCKG 1 cut(s) 2651
MspCI CTTAAG 1 cut(s) 3128
MspI CCGG 4 cut(s) 411, 527, 2972, 3732
MunI CAATTG 2 cut(s) 1848, 3241
Mva1269I GAATGC 6 cut(s) 1489, 1960, 2377, 3084, 3143, 3631
MvnI CGCG 1 cut(s) 3030
MwoI GCNNNNNNNGC 7 cut(s) 626, 2514, 2523, 2648, 2838, 3036, 3131
NciI CCSGG 1 cut(s) 528
NlaIV GGNNCC 5 cut(s) 792, 1987, 2965, 3649, 3730
NmuCI GTSAC 9 cut(s) 208, 215, 337, 403, 442, 478, 539, 1616, 3910
NsiI ATGCAT 3 cut(s) 2238, 3086, 3313
NspI RCATGY 4 cut(s) 512, 2028, 2240, 2940
NspV TTCGAA 1 cut(s) 1214
OliI CACNNNNGTG 1 cut(s) 1745
PaeI GCATGC 2 cut(s) 2240, 2940
PagI TCATGA 1 cut(s) 3088
PaqCI CACCTGC 1 cut(s) 527
PctI GAATGC 6 cut(s) 1489, 1960, 2377, 3084, 3143, 3631
PdmI GAANNNNTTC 2 cut(s) 2101, 2597
PflMI CCANNNNNTGG 2 cut(s) 939, 1550
PfoI TCCNGGA 2 cut(s) 2010, 3577
PinAI ACCGGT 1 cut(s) 3731
PleI GAGTC 7 cut(s) 222, 485, 1301, 2066, 2319, 2565, 2895
PpsI GAGTC 7 cut(s) 222, 485, 1301, 2066, 2319, 2565, 2895
Ppu21I YACGTR 2 cut(s) 1874, 3626
PpuMI RGGWCCY 1 cut(s) 790
PshAI GACNNNNGTC 3 cut(s) 102, 795, 2006
PshBI ATTAAT 1 cut(s) 3042
PsiI TTATAA 2 cut(s) 252, 2210
Psp124BI GAGCTC 2 cut(s) 2281, 2811
Psp5II RGGWCCY 1 cut(s) 790
PspEI GGTNACC 2 cut(s) 539, 2494
PspFI CCCAGC 1 cut(s) 3753
PspN4I GGNNCC 5 cut(s) 792, 1987, 2965, 3649, 3730
PspPI GGNCC 5 cut(s) 413, 790, 1011, 1240, 2008
PspPPI RGGWCCY 1 cut(s) 790
PsrI GAACNNNNNNTAC 2 cut(s) 1369, 1401
PstI CTGCAG 2 cut(s) 990, 2805
PstNI CAGNNNCTG 1 cut(s) 2546
PsuI RGATCY 4 cut(s) 305, 1036, 1123, 3874
PvuII CAGCTG 1 cut(s) 2651
RsaI GTAC 8 cut(s) 109, 977, 1987, 2956, 3239, 3522, 3608, 3772
RsaNI GTAC 8 cut(s) 108, 976, 1986, 2955, 3238, 3521, 3607, 3771
RseI CAYNNNNRTG 6 cut(s) 36, 1659, 1745, 2167, 3218, 3335
SacI GAGCTC 2 cut(s) 2281, 2811
SalI GTCGAC 1 cut(s) 1999
Sau96I GGNCC 5 cut(s) 413, 790, 1011, 1240, 2008
SchI GAGTC 7 cut(s) 223, 486, 1301, 2066, 2320, 2566, 2896
SduI GDGCHC 5 cut(s) 66, 181, 2281, 2678, 2811
SexAI ACCWGGT 1 cut(s) 388
SfcI CTRYAG 4 cut(s) 986, 2382, 2801, 3321
SfuI TTCGAA 1 cut(s) 1214
SinI GGWCC 3 cut(s) 413, 790, 2008
SmiI ATTTAAAT 1 cut(s) 2167
SmiMI CAYNNNNRTG 6 cut(s) 36, 1659, 1745, 2167, 3218, 3335
SmlI CTYRAG 1 cut(s) 3128
SmoI CTYRAG 1 cut(s) 3128
SpeI ACTAGT 2 cut(s) 110, 1247
SphI GCATGC 2 cut(s) 2240, 2940
SsiI CCGC 7 cut(s) 943, 1401, 2933, 3134, 3290, 3365, 3671
SspI AATATT 2 cut(s) 1060, 2246
SspMI CTAG 8 cut(s) 111, 468, 486, 1248, 1320, 2253, 3152, 3858
SstI GAGCTC 2 cut(s) 2281, 2811
StyI CCWWGG 2 cut(s) 297, 1834
SwaI ATTTAAAT 1 cut(s) 2167
TaiI ACGT 7 cut(s) 410, 485, 1876, 3265, 3500, 3628, 3814
TaqI TCGA 8 cut(s) 53, 366, 687, 1214, 2000, 2834, 2885, 3744
TaqII GACCGA 1 cut(s) 80
TatI WGTACW 1 cut(s) 3237
TauI GCSGC 2 cut(s) 2936, 3367
TscAI CASTG 8 cut(s) 71, 175, 447, 685, 1752, 2350, 2918, 3292
TseFI GTSAC 9 cut(s) 208, 215, 337, 403, 442, 478, 539, 1616, 3910
TseI GCWGC 8 cut(s) 836, 985, 3036, 3473, 3589, 3795, 3952, 3955
Tsp45I GTSAC 9 cut(s) 208, 215, 337, 403, 442, 478, 539, 1616, 3910
TspGWI ACGGA 3 cut(s) 744, 2976, 3380
TspRI CASTG 8 cut(s) 71, 175, 447, 685, 1752, 2350, 2918, 3292
Van91I CCANNNNNTGG 2 cut(s) 939, 1550
Vha464I CTTAAG 1 cut(s) 3128
VpaK11BI GGWCC 3 cut(s) 413, 790, 2008
VspI ATTAAT 1 cut(s) 3042
XagI CCTNNNNNAGG 1 cut(s) 393
XapI RAATTY 7 cut(s) 1370, 1427, 1672, 2018, 3268, 3698, 3842
XbaI TCTAGA 1 cut(s) 2252
XceI RCATGY 4 cut(s) 512, 2028, 2240, 2940
XcmI CCANNNNNNNNNTGG 2 cut(s) 2087, 3484
XmiI GTMKAC 1 cut(s) 2000
XmnI GAANNNNTTC 2 cut(s) 2101, 2597
XspI CTAG 8 cut(s) 111, 468, 486, 1248, 1320, 2253, 3152, 3858
Zsp2I ATGCAT 3 cut(s) 2238, 3086, 3313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.