pycom11g23000

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0005139
Physical Location & Seq
Forward (+)
26014769 .. 26021522
6754 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g23000.5

Sequence Viewer

Length: 3978 bp
ATGTTTCTCAGCAAAGAAATTGACGATTTGCATGATGATGGGTTTGAAGGATCGAAAACTGAGCACTGTATATTCACAGAGGTTTTCTTTGGTCAAGACATTGGTCGCACTAGTAACAGATGTCTTGTTACTGGAATGATTAACTTTGAATGTGAGTCAAGTAAGAACACTGATGCAGCACTGTCTCCAAACAGTGAAAACTCAGTTGTAACAAGTCATTCATCCCCTAAAAGTACCTGTTTAGAGGAGTTTTATAATGCAACCAAAGAAATTAGAGAAACCTCCAGACCAGGGTTCTCCTTGGACAGGTCTGCTATGTTAGATAGGAATGGTGATGATGCGGCTGTTAAGAGAATGAAGTTTTCAGCTGATGAGCTTCCTAATACTAAACCTGGTTTAGGAAAGGTCATGTCTGGTCCAGCTTCAAACTCTGTCTCTGAAACAGTGACATTTCGTTTGGTTGAATCTTCTAGCCAGGGAGTCACGTCTAGTTGCTATCTGTTTAAGCAACATGCAGAACTTAACAAACAAGGTGTAATAGGTGACCCAGATGTTTCAAAGTGCAGATTGCCAAACTCAAGTGGGGATGATAGAAAGGAAGTCTGCCTAAGTAAAGCTATTGCTTCGCCTGTTTCACATGAGAACTTCTCGGCCAGGCTTCTGGCTGCAAGTCCATTAGCCACTGTTAAGGATAAGTCCAGAACTACTCTACATGCCGAGGGAAAGCCCAACGGATTTGAATCTCCTGTTTTGGATGTATCCAACGTGGCCTTGAAGACAAAGAATGGTAAGGATCCTCGTCCGCTTCTCCAATATCATGTTGCTCGCTTGCTTGAAGCTGCTGGATGGCATATTGAGAGGCAAAAAAGAGCTAGTAGAAATTATATGGAGACTGTTTATATAACACCTAAGGGAAGGTTAATTCGTGAACTCCCCAAAGCTTGGCGGTTATGTGGAGAACTTTTGTTAGCAGATGGGTACAGTATGCTGCAGGAAGATGATACGAAGGAGTGGGCCAATATCAGTCAGTTCTGGTCAGATCTGTCTGGTGCTATAGTAAGTATTGAGAAGGAGATGCATCATCCACAACCTACTACTGTGTTGGCTTACTGCTGGAGGCTTTTAGATCCTTTTGTGGTTGTCGTGTTTATTGCGAGAAAGATTGGTAGTCTAAGAAAGGGAGAGATAGTTAACGCAACTCAAAGTATAGTTGATTCAAACCATAAGACTGATTCTGCTTTGGCCTTGAATTGTGCCGATAACATAGAAGATCATTTTACTAAGGAAGATGTGTCAGCTCCCCACCGTGATTCCACATTGGCGAGGAAGGGTTTGGCAGTTTCTGAGGGAGATTACAATGCCTGTGGGAAATCTGGTAATGAATGTTCCTCAAAACATTGTGGACAGACTAGTAATGAGGAAGTGAAATTTCGTATGGGACTATCTATTTATTCAGCTAATGCAGAAAGTCATTGCTTGGTGAATGCTGGTAATAGAGTTGAAAATGGGTTCTCTGGGAGAATGACATGTCCTGATTCCTTACCAGTTTGTGGGTCTGGTGGCACCTGCATTCATTCAGCTACTCATCGAGATGAAACTTCCAGAAACTGTAACAATGTGCATGGGGGTTCTGAAGCTGTATCCCCTCACCAAGATAGCAATGCAAATTCCCCTAGCTCTAATAAACAGAGTTCTGGACTTGATTTAGAAACACCTAAGGAAATTATGGTAGACACATCAGTGGATTATTCCGAGGAAAAAGATGAATCCAAGGAAGGCAAGGTTGATGATAAGCTGGAAAGTGCTCAGAAGGGATCACTAGATAACCAAGGAAACTGCATAGTTGATGTTCTGAAAAGAAAAACGCGTAGGAAGTCGAAGAAGATATCTGAAATTGAACCAAGCACGTTATACCGAAGAAACCTTTTAGATTTTACTTCAAGTGGTGCTGATTCACAGTGTATTGATGCTAATGGTACCCAGTCAAAGTTGAAAGAGGCCCAGGATGAATTTACAGGCAATAAAAGATGCAAAGGAAGTCGCAAGATGCCCTTACCTCCCGACTCTTGCCTGCAACAGATTGGGAGGAAAAGTTCAAAGTTGATGAAGATTTCTCTTGAATGCTATGATTTTCAGACTGGAAAAAGGAAATCATTTAATTGCCAAATTGAAGATGATGACTTGTTGGTTTCAGCTATTATCAAAAACAAAGATTTTAGGACAACTCATGCTCAGTGTTCGTCTAGAAAGAAAGCCTACAAATCAAGAGCTCATAAGAAAGGCAAAACTAAAATCCGAAAGAGTAGTTGCAAGTTGCTTCCAAGAAGCCTGGGCAGTGGGGGGAAGCACTTTAAGGATGGAAAGTGGCATTCTGTAGGAGTGAAAACAGTTTTGTCTTGGTTAATTGATGCTGGTGTCATATCTCTAGATGATGTGATTCAGTATCGAAATCCCAAGGATGGCGGTGTCCTTATAGATGGTCTGGTTACGAGGGACGGCATTTTTTGCAAGTGTTGCAGTAAAGTAGTTTCAGTTTCTGAGTTCAAGAGTCATGGTGGGTTCAAACAGAATCGTCCATGTTTGAATCTTTTCATGGAATCTGGTCAATCCTTCACCTTATGCCAGCTTCAAGCTTGGTCAGCTGAATACAAGACCAGGAAAAAGAGCAGCCAAGTCGTGCGAGATGATGAAAATGATCAAAATGATGATTCGTGTGGAATTTGTGGTGATGGTGGCGAGTTGATATGCTGTGATAATTGCCCATCTACTTTTCATCAGACTTGTTTGTCTCTGCAGGAGCTCCCTGAAGGCAGTTGGTATTGCTCAAATTGCACCTGTTGGATATGTGGTGATTTCGTCAATGACAAAGGGGCACCAAGTCCTGATGGATTTAAGTGTTCACAGTGTGAGCACAAATATCATGATGCATGCCTGAAAGAAAAATGGACATACGGAGCGATACCAGATACTTTTTTCTGTAACAGAAGCTGTCAGGAGGCTTATTCTGGTCTTCAATCTCGCGTTGGCTGCATTAATCATTTTGCTGATGGCTTTTCATGGACACTGCTTAGATGCATTCGTGATGACCAAAAGGATCATTCTGCTCAACGATTTGCACTGAAGGCAGAATGCAATACAAAGCTAGCTGTTTCTCTTACAATAATGGAGGAATGCTTTTTGTCAATGGTTGATCCTAGAACAGGCATAGACATGATACCCCATGTCTTATACAACTGGGGGTCAGAGTTTGCACGTTTGAATTTTCAAGGCTTTTACACTGCAGTCTTGGAGAAAGATGATGCGTTGGTATCTGTAGCATCCATCAGGGTGCATGGAACAACAGTTGCAGAGATGCCGCTTATTGCAACTTGCAGTGAATTCCGTCGCCAGGGAATGTGCCGACGCCTCCTGACTGCGATTGAAGAGATGCTAATATCTTTTAAGGTTGAAAAGCTTGTCGTAGCTGCCATTCCCGATTTGGTTGAGACATGGACACAGGGTTTTGGCTTTGTACCCGTAGAAGGCGATGAAAAGCGTGGTTTGGACAAGATCAACTTGATGGTCTTTCCTGGAACAATGCTGCTCAAAAAACCCTTGTACGAAAATCAAAAAGCACATAGGCGTTCCAAAATGGAAACCAACAGAGTTGCTGCTGATGCGGAGTTAGAAGTCGGCGCTGAAGCTGAATTCAACGATGGTATCCATCCATTCGAGGGAACCGGTTGTCAGATCGAAGAAGGCTGGGAATCCGAACTTGATACCAAGGAAGCGGAAGTCGGAGTTAAGGGAAGCAAGAATGCGCAAGCAGAAGTAGGTGAAGCCGAAGGAAGCACTTTGCAGGGCGAATTTTCAAAGCTGTCTGTGGAAGAAGTAGCAGAACTAAAATTAGAAAATAGCGACGGGCCGGAAACCGTGGCTAATGTTGAATCCTTGAAAATGTATGATGAAATACAGCAGCAGCTTCCTTTGGACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1326

Amino Acids

146.46

Weight (kDa)

5.95

Isoelectric Point (pI)

42.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AarI CACCTGC 1 cut(s) 1574
AasI GACNNNNNNGTC 1 cut(s) 2785
Acc16I TGCGCA 1 cut(s) 3801
Acc36I ACCTGC 1 cut(s) 1574
Acc65I GGTACC 1 cut(s) 1976
AccB1I GGYRCC 3 cut(s) 1562, 1976, 2872
AccB7I CCANNNNNTGG 2 cut(s) 942, 1550
AccI GTMKAC 1 cut(s) 1731
AccII CGCG 2 cut(s) 1867, 3021
AciI CCGC 7 cut(s) 341, 803, 946, 2463, 3356, 3659, 3770
AclWI GGATC 7 cut(s) 58, 788, 801, 1121, 1822, 3102, 3185
AcoI YGGCCR 1 cut(s) 651
AcsI RAATTY 8 cut(s) 1427, 1666, 2009, 2718, 3259, 3377, 3686, 3845
AcuI CTGAAG 4 cut(s) 1653, 2826, 3140, 3699
AcyI GRCGYC 1 cut(s) 3403
AdeI CACNNNGTG 1 cut(s) 2906
AfaI GTAC 5 cut(s) 235, 980, 1978, 3513, 3599
AflIII ACRYGT 2 cut(s) 1526, 1865
AgeI ACCGGT 1 cut(s) 3719
AhlI ACTAGT 2 cut(s) 110, 1409
AjiI CACGTC 1 cut(s) 486
AjnI CCWGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
AleI CACNNNNGTG 1 cut(s) 1739
AloI GAACNNNNNNTCC 2 cut(s) 2077, 2109
Alw21I GWGCWC 5 cut(s) 66, 1807, 2272, 2802, 2913
Alw26I GTCTC 5 cut(s) 189, 439, 884, 2793, 3479
AlwI GGATC 7 cut(s) 58, 788, 801, 1121, 1822, 3102, 3185
AlwNI CAGNNNCTG 4 cut(s) 1343, 1608, 2537, 2988
AoxI GGCC 6 cut(s) 651, 768, 1014, 1242, 1998, 3902
ApoI RAATTY 8 cut(s) 1427, 1666, 2009, 2718, 3259, 3377, 3686, 3845
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AseI ATTAAT 1 cut(s) 3033
AsiGI ACCGGT 1 cut(s) 3719
Asp700I GAANNNNTTC 1 cut(s) 2588
Asp718I GGTACC 1 cut(s) 1976
AspLEI GCGC 2 cut(s) 3677, 3802
AspS9I GGNCC 4 cut(s) 416, 1014, 1999, 3902
AsuHPI GGTGA 8 cut(s) 344, 554, 1492, 1640, 2605, 2738, 2861, 3827
AsuNHI GCTAGC 1 cut(s) 3142
AvaII GGWCC 1 cut(s) 416
AxyI CCTNAGG 2 cut(s) 909, 1716
BaeGI GKGCMC 1 cut(s) 2875
BaeI ACNNNNGTAYC 2 cut(s) 3682, 3715
BamHI GGATCC 1 cut(s) 793
BanI GGYRCC 3 cut(s) 1562, 1976, 2872
BanII GRGCYC 2 cut(s) 2272, 2802
BbsI GAAGAC 2 cut(s) 782, 3002
Bbv12I GWGCWC 5 cut(s) 66, 1807, 2272, 2802, 2913
BceAI ACGGC 1 cut(s) 2512
BcgI CGANNNNNNTGC 2 cut(s) 2656, 2690
BciT130I CCWGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
BciVI GTATCC 3 cut(s) 769, 1651, 3710
BclI TGATCA 1 cut(s) 2695
BcoDI GTCTC 5 cut(s) 189, 439, 884, 2793, 3479
BcuI ACTAGT 2 cut(s) 110, 1409
BfmI CTRYAG 6 cut(s) 989, 1053, 2373, 2792, 3279, 3312
BfoI RGCGCY 1 cut(s) 3678
BfuAI ACCTGC 1 cut(s) 1574
BfuI GTATCC 3 cut(s) 769, 1651, 3710
BglII AGATCT 1 cut(s) 1039
Bme1390I CCNGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
Bme18I GGWCC 1 cut(s) 416
BmgBI CACGTC 1 cut(s) 486
BmgT120I GGNCC 4 cut(s) 416, 1014, 1999, 3902
BmiI GGNNCC 5 cut(s) 795, 1564, 1978, 2874, 3718
BmrFI CCNGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
BmrI ACTGGG 2 cut(s) 1975, 3244
BmtI GCTAGC 1 cut(s) 3146
BmuI ACTGGG 2 cut(s) 1975, 3244
BoxI GACNNNNGTC 1 cut(s) 102
BpiI GAAGAC 2 cut(s) 782, 3002
BplI GAGNNNNNCTC 2 cut(s) 632, 664
BpmI CTGGAG 2 cut(s) 268, 1135
BpuEI CTTGAG 1 cut(s) 562
BsaBI GATNNNNATC 1 cut(s) 739
BsaHI GRCGYC 1 cut(s) 3403
BsaWI WCCGGW 1 cut(s) 3719
BsaXI ACNNNNNCTCC 4 cut(s) 1173, 1203, 2077, 2107
Bse118I RCCGGY 1 cut(s) 3719
Bse1I ACTGG 5 cut(s) 136, 1544, 1981, 2143, 3239
Bse21I CCTNAGG 2 cut(s) 909, 1716
Bse3DI GCAATG 2 cut(s) 1471, 1666
Bse8I GATNNNNATC 1 cut(s) 739
BseBI CCWGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
BseJI GATNNNNATC 1 cut(s) 739
BseMI GCAATG 2 cut(s) 1471, 1666
BseMII CTCAG 7 cut(s) 22, 51, 216, 1335, 1820, 2246, 2529
BseNI ACTGG 5 cut(s) 136, 1544, 1981, 2143, 3239
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 2875
BseYI CCCAGC 1 cut(s) 3741
BsgI GTGCAG 1 cut(s) 583
Bsh1236I CGCG 2 cut(s) 1867, 3021
BshFI GGCC 6 cut(s) 653, 770, 1016, 1244, 2000, 3904
BshNI GGYRCC 3 cut(s) 1562, 1976, 2872
BshTI ACCGGT 1 cut(s) 3719
BsiHKAI GWGCWC 5 cut(s) 66, 1807, 2272, 2802, 2913
BsiSI CCGG 2 cut(s) 3720, 3905
BslFI GGGAC 2 cut(s) 1452, 2507
BsmAI GTCTC 5 cut(s) 189, 439, 884, 2793, 3479
BsmFI GGGAC 2 cut(s) 1452, 2507
BsmI GAATGC 8 cut(s) 1489, 1569, 2126, 2368, 3075, 3134, 3176, 3802
BsnI GGCC 6 cut(s) 653, 770, 1016, 1244, 2000, 3904
Bsp1286I GDGCHC 6 cut(s) 66, 1807, 2272, 2802, 2875, 2913
BspACI CCGC 7 cut(s) 341, 803, 946, 2463, 3356, 3659, 3770
BspANI GGCC 6 cut(s) 653, 770, 1016, 1244, 2000, 3904
BspCNI CTCAG 7 cut(s) 21, 52, 215, 1336, 1819, 2245, 2530
BspFNI CGCG 2 cut(s) 1867, 3021
BspHI TCATGA 1 cut(s) 2920
BspLI GGNNCC 5 cut(s) 795, 1564, 1978, 2874, 3718
BspMAI CTGCAG 3 cut(s) 993, 2796, 3283
BspMI ACCTGC 1 cut(s) 1574
BspOI GCTAGC 1 cut(s) 3146
BspPI GGATC 7 cut(s) 58, 788, 801, 1121, 1822, 3102, 3185
BspT107I GGYRCC 3 cut(s) 1562, 1976, 2872
BsrDI GCAATG 2 cut(s) 1471, 1666
BsrFI RCCGGY 1 cut(s) 3719
BsrI ACTGG 5 cut(s) 136, 1544, 1981, 2143, 3239
BssAI RCCGGY 1 cut(s) 3719
BssNI GRCGYC 1 cut(s) 3403
BssT1I CCWWGG 5 cut(s) 300, 1770, 1828, 2454, 3762
Bst2UI CCWGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
Bst6I CTCTTC 1 cut(s) 3417
BstACI GRCGYC 1 cut(s) 3403
BstAPI GCANNNNNTGC 2 cut(s) 2505, 2514
BstC8I GCNNGC 7 cut(s) 826, 830, 2072, 2624, 2929, 3144, 3804
BstDSI CCRYGG 1 cut(s) 3912
BstEII GGTNACC 1 cut(s) 542
BstENI CCTNNNNNAGG 3 cut(s) 304, 396, 3198
BstFNI CGCG 2 cut(s) 1867, 3021
BstH2I RGCGCY 1 cut(s) 3678
BstHHI GCGC 2 cut(s) 3677, 3802
BstMAI GTCTC 5 cut(s) 189, 439, 884, 2793, 3479
BstMWI GCNNNNNNNGC 7 cut(s) 2505, 2514, 2639, 2829, 3027, 3122, 3656
BstNI CCWGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
BstNSI RCATGY 4 cut(s) 515, 716, 1530, 2931
BstPAI GACNNNNGTC 1 cut(s) 102
BstPI GGTNACC 1 cut(s) 542
BstSCI CCNGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
BstSFI CTRYAG 6 cut(s) 989, 1053, 2373, 2792, 3279, 3312
BstSLI GKGCMC 1 cut(s) 2875
BstUI CGCG 2 cut(s) 1867, 3021
BstV2I GAAGAC 2 cut(s) 782, 3002
BstX2I RGATCY 3 cut(s) 793, 1039, 1126
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 3 cut(s) 793, 1039, 1126
Bsu36I CCTNAGG 2 cut(s) 909, 1716
BsuI GTATCC 3 cut(s) 769, 1651, 3710
BsuRI GGCC 6 cut(s) 653, 770, 1016, 1244, 2000, 3904
BtgI CCRYGG 1 cut(s) 3912
BtgZI GCGATG 1 cut(s) 3540
BtrI CACGTC 1 cut(s) 486
BtsI GCAGTG 4 cut(s) 2341, 3062, 3276, 3379
BveI ACCTGC 1 cut(s) 1574
Cac8I GCNNGC 7 cut(s) 826, 830, 2072, 2624, 2929, 3144, 3804
CaiI CAGNNNCTG 4 cut(s) 1343, 1608, 2537, 2988
CciI TCATGA 1 cut(s) 2920
CfoI GCGC 2 cut(s) 3677, 3802
Cfr10I RCCGGY 1 cut(s) 3719
Cfr13I GGNCC 4 cut(s) 416, 1014, 1999, 3902
CseI GACGC 1 cut(s) 3411
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 5 cut(s) 234, 979, 1977, 3512, 3598
CspAI ACCGGT 1 cut(s) 3719
CspCI CAANNNNNGTGG 2 cut(s) 1345, 1380
CviQI GTAC 5 cut(s) 234, 979, 1977, 3512, 3598
DraIII CACNNNGTG 1 cut(s) 2906
DrdI GACNNNNNNGTC 1 cut(s) 2785
DseDI GACNNNNNNGTC 1 cut(s) 2785
EaeI YGGCCR 1 cut(s) 651
Eam1104I CTCTTC 1 cut(s) 3417
EarI CTCTTC 1 cut(s) 3417
Ecl136II GAGCTC 2 cut(s) 2270, 2800
Eco130I CCWWGG 5 cut(s) 300, 1770, 1828, 2454, 3762
Eco24I GRGCYC 2 cut(s) 2272, 2802
Eco32I GATATC 1 cut(s) 1887
Eco47I GGWCC 1 cut(s) 416
Eco53kI GAGCTC 2 cut(s) 2270, 2800
Eco57I CTGAAG 4 cut(s) 1653, 2826, 3140, 3699
Eco81I CCTNAGG 2 cut(s) 909, 1716
Eco91I GGTNACC 1 cut(s) 542
EcoICRI GAGCTC 2 cut(s) 2270, 2800
EcoNI CCTNNNNNAGG 3 cut(s) 304, 396, 3198
EcoO65I GGTNACC 1 cut(s) 542
EcoRI GAATTC 2 cut(s) 3377, 3686
EcoRII CCWGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
EcoRV GATATC 1 cut(s) 1887
EcoT14I CCWWGG 5 cut(s) 300, 1770, 1828, 2454, 3762
EcoT22I ATGCAT 3 cut(s) 1080, 2929, 3077
EcoT38I GRGCYC 2 cut(s) 2272, 2802
ErhI CCWWGG 5 cut(s) 300, 1770, 1828, 2454, 3762
FalI AAGNNNNNCTT 4 cut(s) 2036, 2068, 3539, 3571
FaqI GGGAC 2 cut(s) 1452, 2507
FbaI TGATCA 1 cut(s) 2695
FblI GTMKAC 1 cut(s) 1731
FriOI GRGCYC 2 cut(s) 2272, 2802
FspI TGCGCA 1 cut(s) 3801
GlaI GCGC 2 cut(s) 3676, 3801
GsaI CCCAGC 1 cut(s) 3745
GsuI CTGGAG 2 cut(s) 268, 1135
HaeII RGCGCY 1 cut(s) 3678
HaeIII GGCC 6 cut(s) 653, 770, 1016, 1244, 2000, 3904
HapII CCGG 2 cut(s) 3720, 3905
HgaI GACGC 1 cut(s) 3411
HhaI GCGC 2 cut(s) 3677, 3802
Hin1I GRCGYC 1 cut(s) 3403
Hin6I GCGC 2 cut(s) 3675, 3800
HinP1I GCGC 2 cut(s) 3675, 3800
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HindIII AAGCTT 3 cut(s) 939, 2631, 3452
HpaI GTTAAC 1 cut(s) 1192
HpaII CCGG 2 cut(s) 3720, 3905
HphI GGTGA 8 cut(s) 344, 554, 1492, 1640, 2605, 2738, 2861, 3827
Hpy166II GTNNAC 5 cut(s) 929, 1192, 1403, 1732, 2900
Hpy8I GTNNAC 5 cut(s) 929, 1192, 1403, 1732, 2900
Hpy99I CGWCG 3 cut(s) 3387, 3405, 3902
HpyCH4IV ACGT 4 cut(s) 485, 765, 1907, 3253
HpyF10VI GCNNNNNNNGC 7 cut(s) 2505, 2514, 2639, 2829, 3027, 3122, 3656
HpySE526I ACGT 4 cut(s) 485, 765, 1907, 3253
Hsp92I GRCGYC 1 cut(s) 3403
HspAI GCGC 2 cut(s) 3675, 3800
KpnI GGTACC 1 cut(s) 1980
Ksp22I TGATCA 1 cut(s) 2695
KspAI GTTAAC 1 cut(s) 1192
LmnI GCTCC 4 cut(s) 1303, 2797, 2805, 2954
MabI ACCWGGT 1 cut(s) 391
MaeII ACGT 4 cut(s) 485, 765, 1907, 3253
MaeIII GTNAC 9 cut(s) 113, 127, 208, 445, 481, 542, 1610, 2485, 2978
MflI RGATCY 3 cut(s) 793, 1039, 1126
MhlI GDGCHC 6 cut(s) 66, 1807, 2272, 2802, 2875, 2913
MluI ACGCGT 1 cut(s) 1865
MlyI GAGTC 4 cut(s) 164, 489, 2057, 2557
MmeI TCCRAC 3 cut(s) 786, 2819, 3757
Mph1103I ATGCAT 3 cut(s) 1080, 2929, 3077
MroXI GAANNNNTTC 1 cut(s) 2588
MslI CAYNNNNRTG 5 cut(s) 36, 1590, 1739, 3209, 3326
MspA1I CMGCKG 2 cut(s) 368, 2642
MspI CCGG 2 cut(s) 3720, 3905
MspR9I CCNGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
Mva1269I GAATGC 8 cut(s) 1489, 1569, 2126, 2368, 3075, 3134, 3176, 3802
MvaI CCWGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
MvnI CGCG 2 cut(s) 1867, 3021
MwoI GCNNNNNNNGC 7 cut(s) 2505, 2514, 2639, 2829, 3027, 3122, 3656
NheI GCTAGC 1 cut(s) 3142
NlaIV GGNNCC 5 cut(s) 795, 1564, 1978, 2874, 3718
NmeAIII GCCGAG 2 cut(s) 629, 742
NmuCI GTSAC 3 cut(s) 445, 481, 542
NsbI TGCGCA 1 cut(s) 3801
NsiI ATGCAT 3 cut(s) 1080, 2929, 3077
NspI RCATGY 4 cut(s) 515, 716, 1530, 2931
OliI CACNNNNGTG 1 cut(s) 1739
PaeI GCATGC 1 cut(s) 2931
PagI TCATGA 1 cut(s) 2920
PaqCI CACCTGC 1 cut(s) 1574
PciI ACATGT 1 cut(s) 1526
PctI GAATGC 8 cut(s) 1489, 1569, 2126, 2368, 3075, 3134, 3176, 3802
PdmI GAANNNNTTC 1 cut(s) 2588
PflMI CCANNNNNTGG 2 cut(s) 942, 1550
PfoI TCCNGGA 1 cut(s) 3568
PinAI ACCGGT 1 cut(s) 3719
PleI GAGTC 4 cut(s) 163, 488, 2057, 2556
PpsI GAGTC 4 cut(s) 163, 488, 2057, 2556
PscI ACATGT 1 cut(s) 1526
PshAI GACNNNNGTC 1 cut(s) 102
PshBI ATTAAT 1 cut(s) 3033
PsiI TTATAA 1 cut(s) 255
Psp124BI GAGCTC 2 cut(s) 2272, 2802
Psp6I CCWGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
PspEI GGTNACC 1 cut(s) 542
PspFI CCCAGC 1 cut(s) 3741
PspGI CCWGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
PspN4I GGNNCC 5 cut(s) 795, 1564, 1978, 2874, 3718
PspPI GGNCC 4 cut(s) 416, 1014, 1999, 3902
PsrI GAACNNNNNNTAC 2 cut(s) 1369, 1401
PstI CTGCAG 3 cut(s) 993, 2796, 3283
PstNI CAGNNNCTG 4 cut(s) 1343, 1608, 2537, 2988
PsuI RGATCY 3 cut(s) 793, 1039, 1126
PvuII CAGCTG 2 cut(s) 368, 2642
RsaI GTAC 5 cut(s) 235, 980, 1978, 3513, 3599
RsaNI GTAC 5 cut(s) 234, 979, 1977, 3512, 3598
RseI CAYNNNNRTG 5 cut(s) 36, 1590, 1739, 3209, 3326
SacI GAGCTC 2 cut(s) 2272, 2802
Sau96I GGNCC 4 cut(s) 416, 1014, 1999, 3902
SchI GAGTC 4 cut(s) 164, 489, 2057, 2557
ScrFI CCNGG 9 cut(s) 291, 393, 476, 655, 2003, 2330, 2656, 3389, 3570
SduI GDGCHC 6 cut(s) 66, 1807, 2272, 2802, 2875, 2913
SexAI ACCWGGT 1 cut(s) 391
SfcI CTRYAG 6 cut(s) 989, 1053, 2373, 2792, 3279, 3312
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 5 cut(s) 36, 1590, 1739, 3209, 3326
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
SpeI ACTAGT 2 cut(s) 110, 1409
SphI GCATGC 1 cut(s) 2931
SsiI CCGC 7 cut(s) 341, 803, 946, 2463, 3356, 3659, 3770
SstI GAGCTC 2 cut(s) 2272, 2802
StyD4I CCNGG 9 cut(s) 289, 391, 474, 653, 2001, 2328, 2654, 3387, 3568
StyI CCWWGG 5 cut(s) 300, 1770, 1828, 2454, 3762
TaiI ACGT 4 cut(s) 488, 768, 1910, 3256
TaqI TCGA 6 cut(s) 53, 1588, 1877, 2446, 3711, 3732
TauI GCSGC 2 cut(s) 344, 3358
TseFI GTSAC 3 cut(s) 445, 481, 542
Tsp45I GTSAC 3 cut(s) 445, 481, 542
TspGWI ACGGA 3 cut(s) 747, 2967, 3371
Van91I CCANNNNNTGG 2 cut(s) 942, 1550
VpaK11BI GGWCC 1 cut(s) 416
VspI ATTAAT 1 cut(s) 3033
XagI CCTNNNNNAGG 3 cut(s) 304, 396, 3198
XapI RAATTY 8 cut(s) 1427, 1666, 2009, 2718, 3259, 3377, 3686, 3845
XbaI TCTAGA 2 cut(s) 2243, 2425
XceI RCATGY 4 cut(s) 515, 716, 1530, 2931
XcmI CCANNNNNNNNNTGG 1 cut(s) 3475
XmiI GTMKAC 1 cut(s) 1731
XmnI GAANNNNTTC 1 cut(s) 2588
Zsp2I ATGCAT 3 cut(s) 1080, 2929, 3077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.