MD11G1260600.v1.1

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005139
Physical Location & Seq
Forward (+)
37465236 .. 37476228
10993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1260600.v1.1.491

Sequence Viewer

Length: 3975 bp
ATGTTTCTCAGCGAAGAAATTGACGATTTGCATGATGATGGGTTTGAAGGATCGAAAACTGAGCACTGTATATTCACAGAGGTTTTCTTTGGTCAAGACATTGGTCGCACTAGTAACAGATGTCTTGTTACTGGAATGATTAACTTTGAATGTGAGTCAAGTAAGAACACTGATGCAGCACTGTCTCCAAACAGTGAAAACTCAGTTGTAACAAGTCACTCATCCCCTAAAAGTACCTGTTTAGAGGAGTTTTATAATGCAACCAAAGAAATTAGAGAAACCTCTGGACCAGGGTTTTCCTTGGACAGGTCTGCTATGTTAGATAGGAATGTTGATGATGCAGCTGTTAAGAGAATGAAGTTTTCAGCTGATGAGCTTCCTAATACTAAACCTGGTTTAGGAAAGGTCACTTCTGGTCCAGCTTCAAACTCTATCTCTGAAACAGTGACATTTCGTTTGGTTGAATCTTCTAGCCAGGGAGTCACATCTAGTTGCTATCTGTTTAAGCAACATGCAGAACTTAACAAACCGGGCGTAATAGGTGACCCAGATGTTTCAAGGTGCAGATTGCCAAATTCAAGTGGGAATGATAGAAAGGAAGTCTGCCTAAGTAAAGCTATTGCTTCACCTGTTGCACATGAGAACTTCTCGGCCAGGCTTCTGGCTGCAAGTCCATTAGCCACGATGGATAAGTCCAGAACTCCTCTACCTGCCGAGGGAAAGCCCAACGGATTTGAATCTCCTGTTTTGGATGTATCCAATGTGGCCTTGAAGACAGAGAATAGTAAGGACCCTCGTCCGCTTCTCCAATATCACATTGGTCGCTTGCTTGGAGCTGCTGGATGGCATATTGAGAGGCAAAAAAGAGCTAGTAGAAATTATATGGAGACTGTTTATATAACACCTAAGGGAAGGTTAATTCGTGAACTCCCCAAAGCTTGGCAGTTATGTGGAGAACTTTTGTTAGCAGATGGGTACAGTATGCTGCAGGAAGACGATACAAAGGAGTGGGCCAATATCAGTCAGTTCTGGTCAGATCTGTCTGGTGCTATGGTAAGTATTGAGAAAGAGATGCATCATCCACAACCTACTACTGTGTTGGCTTACTGCTGGAGGCTTTTAGATCCTTTTGTGGTTGTTGTGTTTATTGCAAAAAAGATTGGTAGTCTAAGAAAGGGAAAGATAGTTAAAGCAACTCAAAGTATAGTTGATTCAAACCATAAGACTGGTTCTGCTTTGGCCTTGAATTGTGCCGATAACATAGAAGATCATTTTCCTAAGGAAGATGTGTCAGCTCCCCACCGTGATTCCACATTGGCGAGGAGGGGTTTGGCAGTTTCTAAGGGAGATTACAATGCCTGTGGGAAATCTGGTAATGAAAGTTCCTCAAAACATTGTGGACAGACTAGTAATGAGGAAGTGAAATCTCTTATGGGACTATCTGTTTATTCAGCTAATGCGGAAAGTCATTGCTTGGTGAATGCTGGTAATAGAGTTGAAAATGGGTTCTCCGGGAAAATGACATGTCCGGATTCCTTACCAGTTTGTGGGTCTGGTGGCACCTGCATTCAGTCAGCTACTCATCGAGATGAAACTTCCAGAAACTGTAACAATGTGCATGGGGGTTCTGAAGCTGTATCCCCTCACCAGGATAGCAATGCAAATTCCCCTAGCTCTAATAAACAGAGTTCTGGACTTGATTTAGAAACACCTAAGGAAATTATGGAAGACACATCAGTGGATTATTCTGAGGAAAAAGATGAATCCAAGGGAGGCAAGGTTGATGATAATCTGGAAAGTGCTCAGAAGGGATCACTAGATAACCAAGGAAACTGCATAGTTGATGTTCTGAAAAGAAAAACGCGTAGGAAGTCTAAAAAGATATCTGAAATTGAACCAAGCACGTTATACCGAAGAAACCTTTTAGATTTTACTTCAAGTGGTGCTGATTCACAGTTTATTGCTGCTAATGATACCCAGTCGAAGTTGAAAGAGGCCCAGGATGAATTTACAGGCAATAAAAGATGCAAAGGAAGTCGCAAGATGCCCTTACCTCCCGACTCTTGCCCACAACAGATTGGGAGGAAAAGTTCAAAGTTGATGAAGATCTCTCGTGAATGCTATGATTTTCAGACTGGAAAAAGGAAATCATTTAACTGCCAAATTGAAGATGATGACTTGTTGGTTTCAGCTATTATCAAAAACAAAGATTTTAGGACAACTCATGCTCGGTATTCGTCTAGAAAGAAAGCCTACAAATCAAGAGCTCATAAGAAAGGCAACACTAAAATCCAAAAGAGTAGTTGCAAGTTGCTTCCAAGAAGCCTGGGCAGTGGGGGGAAGCACTTTAAGGATGGAAAGTGGCATTCTGTAGGAGTGAAAACAGTTTTGTCTTGGTTAATTGATGCTGGTGTCATATCTCTAGATGATGTGATTCAGTATCGAAATCCCAAGGATGGCGGTGTCCTTATAGATGGTCTGGTTACCAGGGACGGCATTTTTTGCAAGTGTTGCAGTAAAGTAGTTTCAGTTTCTGAGTTCAAGAGTCATGGTGGGTTCAAACAGAATCGTCCATGTTTGAATCTTTTCATGGAATCTGGTCAATCCTTCACCTTATGTCAGCTTCAAGCTTGGTCAGCTGAATACAAGACCAGGAAAAAGAGCAGCCAAGTCGTGCGAGATGATGAAAATGATCAAAATGATGATTCGTGTGGAATTTGTGGTGATGGTGGCGAGTTGATATGCTGTGATAATTGCCCATCTACTTTTCATCAGGCTTGTTTGTCTCTGCAGGAGCTCCCTGAAGGCAGTTGGTATTGCTCAAATTGCACCTGTTGGGTATGTGGTGATTTCGTCAATGACAAAGGGGCTTCAAGCCCTGATGGATTGAAGTGTTCACAGTGTGAGCACAAATATCATGACGCATGCCTGAAAGAAAAATGGACATACGGAGCCATACCAGATTCTTTTTTCTGTAACAGAAGCTGTCAGGAGGCTTATTCTGGTCTTCAATCTCGCGTTGGCTGCATTGATCATTTTGCTGATGGCTTTTCGTGGACACTGCTTAGATGCATTCATGATGACCAAAAGGTTCATTCTGCTCAGCGGTTTGCGCTGAAGGCGGAATGCAATACAAAACTAGCTGTTTCTCTTACAATAATGGAGGAATGCTTTTTGTCAATGGTTGATCCTAGAACAGGCATAGACATGATACCCCATGTTTTATACAATAGGGGGTCAGAGTTTGCACGTTTGAATTTTCAAGGCTTTTACACTGCAGTCTTGGAGAAGGATGATGCGTTGGTAACTGTAGCATCCATCAGGGTGCATGGAACAACAGTTGCAGAGATGCCGCTAATTGCAACTTGCAGTAAATTCCGTCGCCAGGGAATGTGCCGACGCCTCCTGACTGCGATTGAAGAGATGCTAATATCTTTCAAGGTTGAAAAGCTTGTCGTAGCTGCCATTCCCGATTTGGTAGAGACATGGACGCAGGGTTTTGGCTTTGTACCGGTAGAAGGCGATGAAAAGCGTAGTTTGGACAAGATCAACTTGATGGTCTTTCCTGGAACAATGCTGCTCATAAAACCCTTGTTCGAAAATCAAAAAGCACATAGGCGTTCTGAAATGGAAACCAACAGAGTTGCTGCTGATGCGGAGTTAGAAGTCGGTGCTGAAGCTGAATTCAACGATGGTGTCCGGCCATTCGAGGGAGCCGGTTGTCAGATCGAAGAAGGCTGGGAATCCGAACTTGACACTGGGGAAGCGGAAGTCGGAGTTAAGGGAAGCAAGAATGCGCAAGCAGAAGTAGGCGAAGCCGAAGGAAGCACTTTGCAGGGCGAATTTTCAAAGCTGTCTGTGGAAGAAGTAGCAGAACTAAAATTAGAAAATAGCGACGGGCCGGAAACCGTGGCTAATGTTGAATCCTTGAAAATGTATGATGAAATACGGCAGCAGCTTCCTTTGGACGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1325

Amino Acids

145.81

Weight (kDa)

5.88

Isoelectric Point (pI)

43.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 826 - 876 1.4e-17 Tify domain binding domain
PHD-1st_NSD PF23011 901 - 943 5.6e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 904 - 943 1.9e-07 PHD-finger
IDM1_C PF23209 1058 - 1196 7.5e-57 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AarI CACCTGC 1 cut(s) 1571
Acc16I TGCGCA 1 cut(s) 3798
Acc36I ACCTGC 2 cut(s) 718, 1571
AccB1I GGYRCC 1 cut(s) 1559
AccB7I CCANNNNNTGG 2 cut(s) 939, 1547
AccII CGCG 2 cut(s) 1864, 3018
AccIII TCCGGA 1 cut(s) 1528
AciI CCGC 8 cut(s) 800, 1460, 2460, 3106, 3122, 3353, 3656, 3767
AclWI GGATC 4 cut(s) 58, 1118, 1819, 3182
AcoI YGGCCR 2 cut(s) 651, 3701
AcsI RAATTY 8 cut(s) 574, 1663, 2006, 2715, 3256, 3374, 3683, 3842
AcuI CTGAAG 4 cut(s) 1650, 2823, 3137, 3696
AcyI GRCGYC 1 cut(s) 3400
AdeI CACNNNGTG 1 cut(s) 2903
AfaI GTAC 3 cut(s) 235, 977, 3510
AflIII ACRYGT 2 cut(s) 1523, 1862
AgeI ACCGGT 1 cut(s) 3511
AhlI ACTAGT 2 cut(s) 110, 1406
AleI CACNNNNGTG 1 cut(s) 1736
AloI GAACNNNNNNTCC 2 cut(s) 2074, 2106
Alw21I GWGCWC 5 cut(s) 66, 1804, 2269, 2799, 2910
Alw26I GTCTC 4 cut(s) 189, 881, 2790, 3476
AlwI GGATC 4 cut(s) 58, 1118, 1819, 3182
AlwNI CAGNNNCTG 3 cut(s) 1605, 2534, 2985
Aor13HI TCCGGA 1 cut(s) 1528
AoxI GGCC 7 cut(s) 651, 765, 1011, 1239, 1995, 3701, 3899
ApoI RAATTY 8 cut(s) 574, 1663, 2006, 2715, 3256, 3374, 3683, 3842
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AsiGI ACCGGT 1 cut(s) 3511
Asp700I GAANNNNTTC 1 cut(s) 2585
AspLEI GCGC 2 cut(s) 3115, 3799
AspS9I GGNCC 6 cut(s) 287, 416, 790, 1011, 1996, 3899
AsuC2I CCSGG 2 cut(s) 531, 1513
AsuHPI GGTGA 7 cut(s) 554, 618, 1489, 1637, 2602, 2735, 2858
AsuII TTCGAA 1 cut(s) 3597
AvaII GGWCC 3 cut(s) 287, 416, 790
AxyI CCTNAGG 3 cut(s) 906, 1278, 1713
BanI GGYRCC 1 cut(s) 1559
BanII GRGCYC 2 cut(s) 2269, 2799
BauI CACGAG 1 cut(s) 2112
BbsI GAAGAC 4 cut(s) 779, 999, 1734, 2999
Bbv12I GWGCWC 5 cut(s) 66, 1804, 2269, 2799, 2910
BceAI ACGGC 2 cut(s) 2509, 3965
BcgI CGANNNNNNTGC 2 cut(s) 2653, 2687
BciVI GTATCC 2 cut(s) 766, 1648
BclI TGATCA 2 cut(s) 2692, 3031
BcnI CCSGG 2 cut(s) 531, 1513
BcoDI GTCTC 4 cut(s) 189, 881, 2790, 3476
BcuI ACTAGT 2 cut(s) 110, 1406
BfmI CTRYAG 5 cut(s) 986, 2370, 2789, 3276, 3309
BfuAI ACCTGC 2 cut(s) 718, 1571
BfuI GTATCC 2 cut(s) 766, 1648
BglII AGATCT 2 cut(s) 1036, 2106
BlpI GCTNAGC 1 cut(s) 3102
Bme18I GGWCC 3 cut(s) 287, 416, 790
BmgT120I GGNCC 6 cut(s) 287, 416, 790, 1011, 1996, 3899
BmiI GGNNCC 4 cut(s) 792, 1561, 2953, 3715
BmrI ACTGGG 2 cut(s) 1972, 3768
BmuI ACTGGG 2 cut(s) 1972, 3768
BoxI GACNNNNGTC 2 cut(s) 102, 795
BpiI GAAGAC 4 cut(s) 779, 999, 1734, 2999
BplI GAGNNNNNCTC 2 cut(s) 632, 664
BpmI CTGGAG 1 cut(s) 1132
Bpu1102I GCTNAGC 1 cut(s) 3102
Bpu14I TTCGAA 1 cut(s) 3597
BpuMI CCSGG 2 cut(s) 531, 1513
BsaBI GATNNNNATC 3 cut(s) 736, 1788, 2105
BsaHI GRCGYC 1 cut(s) 3400
BsaWI WCCGGW 2 cut(s) 1528, 3511
BsaXI ACNNNNNCTCC 4 cut(s) 1764, 1794, 2074, 2104
Bse118I RCCGGY 2 cut(s) 3511, 3716
Bse1I ACTGG 6 cut(s) 136, 1231, 1541, 1978, 2140, 3763
Bse21I CCTNAGG 3 cut(s) 906, 1278, 1713
Bse3DI GCAATG 2 cut(s) 1468, 1663
Bse8I GATNNNNATC 3 cut(s) 736, 1788, 2105
BseAI TCCGGA 1 cut(s) 1528
BseGI GGATG 9 cut(s) 221, 757, 848, 1078, 2008, 2359, 2461, 3298, 3314
BseJI GATNNNNATC 3 cut(s) 736, 1788, 2105
BseMI GCAATG 2 cut(s) 1468, 1663
BseMII CTCAG 7 cut(s) 22, 51, 216, 1740, 1817, 2526, 3116
BseNI ACTGG 6 cut(s) 136, 1231, 1541, 1978, 2140, 3763
BseRI GAGGAG 3 cut(s) 260, 693, 1336
BseYI CCCAGC 1 cut(s) 3738
BsgI GTGCAG 1 cut(s) 583
Bsh1236I CGCG 2 cut(s) 1864, 3018
BshFI GGCC 7 cut(s) 653, 767, 1013, 1241, 1997, 3703, 3901
BshNI GGYRCC 1 cut(s) 1559
BshTI ACCGGT 1 cut(s) 3511
BsiHKAI GWGCWC 5 cut(s) 66, 1804, 2269, 2799, 2910
BsiSI CCGG 7 cut(s) 530, 1512, 1529, 3512, 3700, 3717, 3902
BslFI GGGAC 2 cut(s) 1449, 2504
BsmAI GTCTC 4 cut(s) 189, 881, 2790, 3476
BsmFI GGGAC 2 cut(s) 1449, 2504
BsmI GAATGC 8 cut(s) 1486, 1566, 2123, 2365, 3072, 3131, 3173, 3799
BsnI GGCC 7 cut(s) 653, 767, 1013, 1241, 1997, 3703, 3901
Bsp119I TTCGAA 1 cut(s) 3597
Bsp1286I GDGCHC 5 cut(s) 66, 1804, 2269, 2799, 2910
Bsp13I TCCGGA 1 cut(s) 1528
Bsp1720I GCTNAGC 1 cut(s) 3102
BspACI CCGC 8 cut(s) 800, 1460, 2460, 3106, 3122, 3353, 3656, 3767
BspANI GGCC 7 cut(s) 653, 767, 1013, 1241, 1997, 3703, 3901
BspCNI CTCAG 7 cut(s) 21, 52, 215, 1741, 1816, 2527, 3115
BspEI TCCGGA 1 cut(s) 1528
BspFNI CGCG 2 cut(s) 1864, 3018
BspHI TCATGA 2 cut(s) 2917, 3076
BspLI GGNNCC 4 cut(s) 792, 1561, 2953, 3715
BspMAI CTGCAG 3 cut(s) 990, 2793, 3280
BspMI ACCTGC 2 cut(s) 718, 1571
BspPI GGATC 4 cut(s) 58, 1118, 1819, 3182
BspT104I TTCGAA 1 cut(s) 3597
BspT107I GGYRCC 1 cut(s) 1559
BsrDI GCAATG 2 cut(s) 1468, 1663
BsrFI RCCGGY 2 cut(s) 3511, 3716
BsrI ACTGG 6 cut(s) 136, 1231, 1541, 1978, 2140, 3763
BssAI RCCGGY 2 cut(s) 3511, 3716
BssNI GRCGYC 1 cut(s) 3400
BssSI CACGAG 1 cut(s) 2112
BssT1I CCWWGG 4 cut(s) 300, 1767, 1825, 2451
Bst2BI CACGAG 1 cut(s) 2112
Bst6I CTCTTC 1 cut(s) 3414
BstACI GRCGYC 1 cut(s) 3400
BstAPI GCANNNNNTGC 2 cut(s) 2502, 2511
BstBI TTCGAA 1 cut(s) 3597
BstC8I GCNNGC 3 cut(s) 827, 2926, 3801
BstDSI CCRYGG 1 cut(s) 3909
BstEII GGTNACC 2 cut(s) 542, 2482
BstENI CCTNNNNNAGG 3 cut(s) 304, 396, 3195
BstF5I GGATG 9 cut(s) 221, 757, 848, 1078, 2008, 2359, 2461, 3298, 3314
BstFNI CGCG 2 cut(s) 1864, 3018
BstHHI GCGC 2 cut(s) 3115, 3799
BstMAI GTCTC 4 cut(s) 189, 881, 2790, 3476
BstMWI GCNNNNNNNGC 8 cut(s) 2502, 2511, 2636, 2826, 3024, 3112, 3119, 3653
BstNSI RCATGY 3 cut(s) 515, 1527, 2928
BstPAI GACNNNNGTC 2 cut(s) 102, 795
BstPI GGTNACC 2 cut(s) 542, 2482
BstSFI CTRYAG 5 cut(s) 986, 2370, 2789, 3276, 3309
BstUI CGCG 2 cut(s) 1864, 3018
BstV2I GAAGAC 4 cut(s) 779, 999, 1734, 2999
BstX2I RGATCY 3 cut(s) 1036, 1123, 2106
BstXI CCANNNNNNTGG 2 cut(s) 661, 1226
BstYI RGATCY 3 cut(s) 1036, 1123, 2106
Bsu36I CCTNAGG 3 cut(s) 906, 1278, 1713
BsuI GTATCC 2 cut(s) 766, 1648
BsuRI GGCC 7 cut(s) 653, 767, 1013, 1241, 1997, 3703, 3901
BtgI CCRYGG 1 cut(s) 3909
BtgZI GCGATG 1 cut(s) 3537
BtsCI GGATG 9 cut(s) 221, 757, 848, 1078, 2008, 2359, 2461, 3298, 3314
BtsI GCAGTG 3 cut(s) 2338, 3059, 3273
BveI ACCTGC 2 cut(s) 718, 1571
Cac8I GCNNGC 3 cut(s) 827, 2926, 3801
CaiI CAGNNNCTG 3 cut(s) 1605, 2534, 2985
CciI TCATGA 2 cut(s) 2917, 3076
CfoI GCGC 2 cut(s) 3115, 3799
Cfr10I RCCGGY 2 cut(s) 3511, 3716
Cfr13I GGNCC 6 cut(s) 287, 416, 790, 1011, 1996, 3899
CseI GACGC 3 cut(s) 2930, 3408, 3499
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 3 cut(s) 234, 976, 3509
CspAI ACCGGT 1 cut(s) 3511
CspCI CAANNNNNGTGG 2 cut(s) 1342, 1377
CviQI GTAC 3 cut(s) 234, 976, 3509
DraIII CACNNNGTG 1 cut(s) 2903
EaeI YGGCCR 2 cut(s) 651, 3701
Eam1104I CTCTTC 1 cut(s) 3414
EarI CTCTTC 1 cut(s) 3414
EciI GGCGGA 1 cut(s) 3137
Ecl136II GAGCTC 2 cut(s) 2267, 2797
Eco130I CCWWGG 4 cut(s) 300, 1767, 1825, 2451
Eco24I GRGCYC 2 cut(s) 2269, 2799
Eco32I GATATC 1 cut(s) 1884
Eco47I GGWCC 3 cut(s) 287, 416, 790
Eco53kI GAGCTC 2 cut(s) 2267, 2797
Eco57I CTGAAG 4 cut(s) 1650, 2823, 3137, 3696
Eco81I CCTNAGG 3 cut(s) 906, 1278, 1713
Eco91I GGTNACC 2 cut(s) 542, 2482
EcoICRI GAGCTC 2 cut(s) 2267, 2797
EcoNI CCTNNNNNAGG 3 cut(s) 304, 396, 3195
EcoO109I RGGNCCY 1 cut(s) 790
EcoO65I GGTNACC 2 cut(s) 542, 2482
EcoRI GAATTC 1 cut(s) 3683
EcoRV GATATC 1 cut(s) 1884
EcoT14I CCWWGG 4 cut(s) 300, 1767, 1825, 2451
EcoT22I ATGCAT 2 cut(s) 1077, 3074
EcoT38I GRGCYC 2 cut(s) 2269, 2799
ErhI CCWWGG 4 cut(s) 300, 1767, 1825, 2451
FalI AAGNNNNNCTT 4 cut(s) 2033, 2065, 3536, 3568
FaqI GGGAC 2 cut(s) 1449, 2504
FbaI TGATCA 2 cut(s) 2692, 3031
FokI GGATG 9 cut(s) 208, 764, 855, 1065, 2015, 2366, 2468, 3301, 3305
FriOI GRGCYC 2 cut(s) 2269, 2799
FspI TGCGCA 1 cut(s) 3798
GlaI GCGC 2 cut(s) 3114, 3798
GsaI CCCAGC 1 cut(s) 3742
GsuI CTGGAG 1 cut(s) 1132
HaeIII GGCC 7 cut(s) 653, 767, 1013, 1241, 1997, 3703, 3901
HapII CCGG 7 cut(s) 530, 1512, 1529, 3512, 3700, 3717, 3902
HgaI GACGC 3 cut(s) 2930, 3408, 3499
HhaI GCGC 2 cut(s) 3115, 3799
Hin1I GRCGYC 1 cut(s) 3400
Hin6I GCGC 2 cut(s) 3113, 3797
HinP1I GCGC 2 cut(s) 3113, 3797
HindIII AAGCTT 3 cut(s) 936, 2628, 3449
HpaII CCGG 7 cut(s) 530, 1512, 1529, 3512, 3700, 3717, 3902
HphI GGTGA 7 cut(s) 554, 618, 1489, 1637, 2602, 2735, 2858
Hpy166II GTNNAC 4 cut(s) 926, 1400, 2897, 3057
Hpy8I GTNNAC 4 cut(s) 926, 1400, 2897, 3057
Hpy99I CGWCG 3 cut(s) 3384, 3402, 3899
HpyCH4IV ACGT 2 cut(s) 1904, 3250
HpyF10VI GCNNNNNNNGC 8 cut(s) 2502, 2511, 2636, 2826, 3024, 3112, 3119, 3653
HpySE526I ACGT 2 cut(s) 1904, 3250
Hsp92I GRCGYC 1 cut(s) 3400
HspAI GCGC 2 cut(s) 3113, 3797
Kpn2I TCCGGA 1 cut(s) 1528
Ksp22I TGATCA 2 cut(s) 2692, 3031
LmnI GCTCC 6 cut(s) 833, 1300, 2794, 2802, 2951, 3713
MabI ACCWGGT 1 cut(s) 391
MaeII ACGT 2 cut(s) 1904, 3250
MflI RGATCY 3 cut(s) 1036, 1123, 2106
MhlI GDGCHC 5 cut(s) 66, 1804, 2269, 2799, 2910
MluI ACGCGT 1 cut(s) 1862
MlyI GAGTC 4 cut(s) 164, 489, 2054, 2554
MmeI TCCRAC 1 cut(s) 3754
Mph1103I ATGCAT 2 cut(s) 1077, 3074
MroI TCCGGA 1 cut(s) 1528
MroXI GAANNNNTTC 1 cut(s) 2585
MslI CAYNNNNRTG 5 cut(s) 36, 1587, 1736, 3206, 3323
MspA1I CMGCKG 4 cut(s) 344, 368, 2639, 3106
MspI CCGG 7 cut(s) 530, 1512, 1529, 3512, 3700, 3717, 3902
Mva1269I GAATGC 8 cut(s) 1486, 1566, 2123, 2365, 3072, 3131, 3173, 3799
MvnI CGCG 2 cut(s) 1864, 3018
MwoI GCNNNNNNNGC 8 cut(s) 2502, 2511, 2636, 2826, 3024, 3112, 3119, 3653
NciI CCSGG 2 cut(s) 531, 1513
NlaIV GGNNCC 4 cut(s) 792, 1561, 2953, 3715
NmeAIII GCCGAG 2 cut(s) 629, 739
NmuCI GTSAC 5 cut(s) 215, 406, 445, 481, 542
NsbI TGCGCA 1 cut(s) 3798
NsiI ATGCAT 2 cut(s) 1077, 3074
NspI RCATGY 3 cut(s) 515, 1527, 2928
NspV TTCGAA 1 cut(s) 3597
OliI CACNNNNGTG 1 cut(s) 1736
PaeI GCATGC 1 cut(s) 2928
PagI TCATGA 2 cut(s) 2917, 3076
PaqCI CACCTGC 1 cut(s) 1571
PciI ACATGT 1 cut(s) 1523
PctI GAATGC 8 cut(s) 1486, 1566, 2123, 2365, 3072, 3131, 3173, 3799
PdmI GAANNNNTTC 1 cut(s) 2585
PflMI CCANNNNNTGG 2 cut(s) 939, 1547
PfoI TCCNGGA 2 cut(s) 1511, 3565
PinAI ACCGGT 1 cut(s) 3511
PleI GAGTC 4 cut(s) 163, 488, 2054, 2553
PpsI GAGTC 4 cut(s) 163, 488, 2054, 2553
PpuMI RGGWCCY 1 cut(s) 790
PscI ACATGT 1 cut(s) 1523
PshAI GACNNNNGTC 2 cut(s) 102, 795
PsiI TTATAA 1 cut(s) 255
Psp124BI GAGCTC 2 cut(s) 2269, 2799
Psp5II RGGWCCY 1 cut(s) 790
PspEI GGTNACC 2 cut(s) 542, 2482
PspFI CCCAGC 1 cut(s) 3738
PspN4I GGNNCC 4 cut(s) 792, 1561, 2953, 3715
PspPI GGNCC 6 cut(s) 287, 416, 790, 1011, 1996, 3899
PspPPI RGGWCCY 1 cut(s) 790
PsrI GAACNNNNNNTAC 2 cut(s) 1366, 1398
PstI CTGCAG 3 cut(s) 990, 2793, 3280
PstNI CAGNNNCTG 3 cut(s) 1605, 2534, 2985
PsuI RGATCY 3 cut(s) 1036, 1123, 2106
PvuII CAGCTG 3 cut(s) 344, 368, 2639
RsaI GTAC 3 cut(s) 235, 977, 3510
RsaNI GTAC 3 cut(s) 234, 976, 3509
RseI CAYNNNNRTG 5 cut(s) 36, 1587, 1736, 3206, 3323
SacI GAGCTC 2 cut(s) 2269, 2799
Sau96I GGNCC 6 cut(s) 287, 416, 790, 1011, 1996, 3899
SchI GAGTC 4 cut(s) 164, 489, 2054, 2554
SduI GDGCHC 5 cut(s) 66, 1804, 2269, 2799, 2910
SexAI ACCWGGT 1 cut(s) 391
SfcI CTRYAG 5 cut(s) 986, 2370, 2789, 3276, 3309
SfuI TTCGAA 1 cut(s) 3597
SinI GGWCC 3 cut(s) 287, 416, 790
SmiMI CAYNNNNRTG 5 cut(s) 36, 1587, 1736, 3206, 3323
SpeI ACTAGT 2 cut(s) 110, 1406
SphI GCATGC 1 cut(s) 2928
SsiI CCGC 8 cut(s) 800, 1460, 2460, 3106, 3122, 3353, 3656, 3767
SstI GAGCTC 2 cut(s) 2269, 2799
StyI CCWWGG 4 cut(s) 300, 1767, 1825, 2451
TaiI ACGT 2 cut(s) 1907, 3253
TaqI TCGA 7 cut(s) 53, 1585, 1982, 2443, 3597, 3708, 3729
TauI GCSGC 1 cut(s) 3355
TseFI GTSAC 5 cut(s) 215, 406, 445, 481, 542
Tsp45I GTSAC 5 cut(s) 215, 406, 445, 481, 542
TspGWI ACGGA 3 cut(s) 744, 2964, 3368
Van91I CCANNNNNTGG 2 cut(s) 939, 1547
VpaK11BI GGWCC 3 cut(s) 287, 416, 790
XagI CCTNNNNNAGG 3 cut(s) 304, 396, 3195
XapI RAATTY 8 cut(s) 574, 1663, 2006, 2715, 3256, 3374, 3683, 3842
XbaI TCTAGA 2 cut(s) 2240, 2422
XceI RCATGY 3 cut(s) 515, 1527, 2928
XcmI CCANNNNNNNNNTGG 2 cut(s) 815, 3472
XmnI GAANNNNTTC 1 cut(s) 2585
Zsp2I ATGCAT 2 cut(s) 1077, 3074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.