RLG00000033300

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005139
Physical Location & Seq
Reverse (-)
25438145 .. 25451969
13825 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033300

Sequence Viewer

Length: 4095 bp
ATGATTGTGGATGTAGCTCTTAATCGTAATGATGAGATAAGGAATGATAAGGAAGAGACTGTGGATAAAGTGGCGTTTCAGTTACTAGAGATGTTTCTCAGCAAAGAAATTGAGGATCTGCATGATGATGACTTTGAGGGATCCAAAACTGAGCACTGTATATTCACAGAGGTTTTCTTTGGTGATGACAGTGCTAGCACGAGTAAGAGATGTCTTGTAACTGGAGTGATTAACTTTGAATGTGAGTCAAGTAAGACCACAGATACATCACTCTCTTCAAACAGTGAAAACTCAATCGTAACGAATCAGTCAAACTCTAAAAATACTTGTTTGGAGGAGTTTTATAATGCTACTGAGGAATCTAGAGAAATTGTTGGACCAGGGTGTTTTCCGGACAGATCCACTTGGCTAGATACGAATGGCGAAGATGTGAGTGCTAAGAGAATGAAGTTTTCAGTTGATGAACCTAGTGCTAAACCTGGTTTAGGAAAGCTAATAGATTCATCCATAGTTTCAAAAGAAATGGTTTCTGGCTTGTCTGGTACTACAGACTCCATTACTGAAACAGTGACATTTCGTTTGGTCGAATCTTCGAGTGAGGGAGTTACATTTAGTTGCTATCTGTTAAAGCAGCCTGCAAAACTTGACAGAGGGGCCATAGTAGATGACTCTGATGTTTCAAAGTGCAGATTACTAACTTCTGATGGGAATGATGGAAAGGAACTATGTGCAAGTAAAGCTGTTGCTTCACCTGTTTCCCAGGAGAGCTTCTCAACCATGCTTTTGGCTACAGGTTCCCCTTTCACTCTATTGGATAAGTTGGGAACTCCTCTAGGTGCTGAGGGAAAACCTGAGGGGTTGGAATCTCCTGCGTTGGATAGATCTGCTATTGCCTTCAAGAAAGACTCTAGTAAGGATCCTCGTCCGCTTCTCCAGTATGATGTCAATCGCTTACTTGAAGCTGCAGGATGGCATATTCAGAGGCGGAAAAGACCTAGTAGAGCTTATATGGAGTCTGTTTATGTAACACCAAAAGGAAGGTTAATTCGTGACTTCCCCAAAGCTTGGCGGTTGTGTGGTGAACTTTTGTTTGCAGATAGGTGCAATTCGCTGCAGAGAGATGATGCAAAGGAATGGTCCGATATTAATCAGTTTTGGTCAGATCTATCTGGTGCTTTAGTAAATTTTGAGAAAGAGATGAATCAACCAGAACCTGCTACTGAATTGGCTTATTGGTGGAGGGTCTTAGATCCCTTTGTGATTGTTGTGTTTATTGAGAGAAAGATTCTTGCTCTGAGAAAAGGAGAGACTGTTAAAGCAACTCAAAGTTTAGCTATTAATACTAATCATAAGGTTTTGGCCTTGACTAATGCGAATAGTATCAAAAATCATCTGGAGAAGGAAGATGTTTCTGCTCCCCTCTGTGATACAACTCTGGCAGGATGGAGTGGATCGGCAGTTTCTGAGGGGAATATTATGGAAGATGTATCAGTGGTTTGTTCTGAGGAAAGAGATGAACTGCTAGGAGGCAAGGCTTATGATAAGCTGGGAGATAATCTGCAGGGGTCTCTGGAGTACCAACAAAAATGCGCAAGTAATGTTGTTTTGAAAAAGAAAATAAGAAGGAAGTGTAAAAAGATTTCTGAAATGGAACCAAGCTCCACTTCTAGTAATAATATTGGTTTGCAATGCATTGATGTCAGTGGTAACCAGTCAAAGTTGAAAGAGGTCGATGGTGACCTTGCAGGCAATAAAAGAAGTAAAGGAAGTTGCAAGAAGTCCTCATCTCTCAACTCCTCCCAGCATGTGGTTGGTGAAACAAGCCCGAAGTCTGTGAAAAATGTTCGTGACTATGATGATTTTAAAACTGGAATGAAGAAACCATCTAGATTTGAAATCAAAGATGATGACTTACTGGTTTCAGCTATCATAAAGAACACAGATTTTAGTGCAAGTCCTGGTCGATGTTCTTCTAGAAAGAAAGGCCGCAAATGTAGAGCTCATAGGAAGCATAAAAGTCAAAAGAGCCATTGCAAGTTGCTTCTTAGAAGCCTGGGTAGTGTAGGAGTAAAAACAGTTTTGTCTTGGATGCTTGATGCTGGGGTCATATTTCTGGATGATGTGATCCAGTATCGGAATACTAAGGATGGTTCTGTTATTAAAGATGGTCTAGTTACCAGGGATGGTATTTTTTGCAAGTGTTGCAGTGAAGTACTTACAGTTTCTGAGTTCAAGATTCATGGTGGGTTCAGACTCAACCGTCCATGTTTAAATCTTTTTCTGGAATCTGGTAAGGCCTTCTCCTTATGCCAGCTTCAAGCCTGGTCAGCTGAATACAAGTCCAGGAAACGGCGGACTCAAGTGGTGCGAGCTGATGAAAATGATGAAAATGATGATTCTTGTGGACTTTGTGGTGAAGGGGGTGAGCTGATATGCTGTGATAACTGCCCCTCTACTTTTCATCTGGCTTGTTTGTCTCTGCAGGAGCTCCCTGAAGGCAGCTGGTATTGTCCAAATTGCACCTGTTGGATATGTGGTCATTTTGTTACCGACAAAGGGGCTTCAAGTGCTTCTGATGGATTTAAATGTTCACAGTGTGAGCATAAATATCATGAGGCTTGTCTGAAAGAACAATCCATACATGTACTGGATGCTTGGTTCTGTGATAGAAGCTGTCAAGAGGTTTACTCAGGTCTTCAGTCTCATGTTGGCTACATTAATCATGTTGTCGATGGCTTTTCATGGACACTTCTTAGATGCATTCGTGATGACCAAAAAGTTCATTCTGCCCAACGGTTAGCACTGAAAGCAGAATGCAATACAAGATTGGCTGTTGCATTTACTGTCATGGAAGAAAGCTTTCTGTCTATGGTGGATCCTAGAACAGGCATAGACATGATACCCCAAGTTTTGTACAGTTGGGGGTCAGAGTTTGCTCGTTTGAATTTTCAAGGCTTCTACACTGCTGTCTTAGAGAAAGATGATGTGCTGATATCTGTAGCATCCATCAGGGTGCATGGAACAACTGTTGCAGAGATGCCACTTATTGCAACTTGCAGTCGATACCGTCGCCAGGGAATGTGCCGACGCCTTCTGACTGCTATTGAAGAGATGCTGATATCTTTTAAGGTGGAAAAGCTTGTAATAGCTGCCATTCCAGATTTGGTGGGGACATGGACAGATGGTTTTGGCTTTATACCTGTGGAAGACAGAGAAAAACAAAGCTTGAACAAGATCAATTTGATGGTTTTTCCTGGAACGGTTTTACTCAAGAAGCCATTATGTGAAAATAAAACAGCATATAGACATCCTGGAACTTGTCATACATCATCCTTGGAAACGGGAGGATTGAGAAAAGACTGTTGTTCTGAAGAAGAGCCCATGGTTGAGTTTGTGCACTCTGATGATACTTGTGGAAACAAAACTGGTGCTGAAGCAGGAATTGAATTTGTAGAAGGTACTAATTTAGAAGAATGTGATGATGGTAGTAAATTATTTATTACTGCTGGTACTGGACCAGTTGGTACATCAGAAGGAAAAAACAAAGCAACTGAGGTAGGTATTCAATCCAGAGATGCCATTGTTGGGTTTGTACAGCAACATGTTGTCCAGTGTTCTGCTAACAAAGCCGGTGCTGAAATGGAAATGGAAAGCAAGAAGTCAGTGGAATCTGAATCGTATGCTGAAACGGGTATTGAGGCTGTAAAGCAATCGGATGGGATATGTCATGCGGACATAGTTGCTGATGCAGTTAAACTTGAAGTCGAGAATTTCCAGGAATTTGAAGCTGTAAAGCAATCAAAAATCTGTTGCCAGATTGAAGCAGGCAATGAATCAGAAATAAGACAGATGACTGGCAAGGATTTGCTGGAATCTGAAGTCGGCAGCGAACCAGAAGGTAGAGTTTTTGAAAGCAATCTTATGCAAGTGGGTGAAGCCAAAATAAGTACTTTGCAGGAGCAATTTTTAAAGCTGTCTTGTGAAGAACCAGCTTCAGCAGTAGAAAATAGCAAGCCGGAAACGGTTACTAGTGTTGAATCCTTGGAAATGTATGATGAAACACAGCTTTCTCTGGATGAACAATCGCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

1365

Amino Acids

150.77

Weight (kDa)

5.24

Isoelectric Point (pI)

41.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 723 - 773 1.5e-15 Tify domain binding domain
PHD-1st_NSD PF23011 798 - 840 4.2e-06 Histone-lysine N-methyltransferase NSD-like, PHD zinc finger 1
PHD PF00628 801 - 840 1.2e-07 PHD-finger
IDM1_C PF23209 954 - 1093 3e-58 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
AasI GACNNNNNNGTC 1 cut(s) 2259
Acc16I TGCGCA 1 cut(s) 1591
Acc36I ACCTGC 1 cut(s) 1222
AccB7I CCANNNNNTGG 2 cut(s) 1065, 1807
AccIII TCCGGA 1 cut(s) 391
AciI CCGC 6 cut(s) 926, 985, 1069, 1987, 2353, 3731
AcsI RAATTY 5 cut(s) 1183, 2944, 3446, 3769, 3779
AcuI CTGAAG 6 cut(s) 2514, 2681, 3390, 3453, 3897, 3978
AcyI GRCGYC 1 cut(s) 3088
AdeI CACNNNGTG 1 cut(s) 2597
AfiI CCNNNNNNNGG 8 cut(s) 485, 1065, 1807, 2349, 2556, 2885, 3073, 3585
AflIII ACRYGT 2 cut(s) 2641, 3601
AhlI ACTAGT 1 cut(s) 4028
AjuI GAANNNNNNNTTGG 2 cut(s) 1648, 1680
AloI GAACNNNNNNTCC 2 cut(s) 2642, 2674
Alw21I GWGCWC 4 cut(s) 156, 2002, 2490, 3399
Alw26I GTCTC 5 cut(s) 50, 1301, 1572, 2481, 2706
Alw44I GTGCAC 1 cut(s) 3395
AlwNI CAGNNNCTG 3 cut(s) 1214, 1463, 2225
Aor13HI TCCGGA 1 cut(s) 391
AoxI GGCC 4 cut(s) 654, 1359, 1984, 2295
ApaLI GTGCAC 1 cut(s) 3395
ApeKI GCWGC 6 cut(s) 631, 962, 1111, 2499, 3149, 3885
ApoI RAATTY 5 cut(s) 1183, 2944, 3446, 3769, 3779
ArsI GACNNNNNNTTYG 4 cut(s) 562, 594, 1121, 1153
AseI ATTAAT 3 cut(s) 1146, 1338, 2718
Asp700I GAANNNNTTC 1 cut(s) 2859
AspLEI GCGC 1 cut(s) 1592
AspS9I GGNCC 4 cut(s) 377, 654, 1137, 3515
AsuHPI GGTGA 8 cut(s) 194, 741, 1091, 1748, 1826, 2426, 2435, 3944
AsuNHI GCTAGC 1 cut(s) 194
AvaII GGWCC 3 cut(s) 377, 1137, 3515
AxyI CCTNAGG 1 cut(s) 852
BaeGI GKGCMC 1 cut(s) 3399
BamHI GGATCC 3 cut(s) 140, 916, 2875
BanII GRGCYC 3 cut(s) 2002, 2490, 3381
BauI CACGAG 1 cut(s) 199
BbsI GAAGAC 2 cut(s) 2687, 3213
Bbv12I GWGCWC 4 cut(s) 156, 2002, 2490, 3399
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 6 cut(s) 643, 949, 1098, 2511, 3136, 3897
BceAI ACGGC 1 cut(s) 2366
BcoDI GTCTC 5 cut(s) 50, 1301, 1572, 2481, 2706
BcuI ACTAGT 1 cut(s) 4028
BfmI CTRYAG 7 cut(s) 546, 789, 963, 1112, 1559, 2480, 2997
BfuAI ACCTGC 1 cut(s) 1222
BglII AGATCT 2 cut(s) 881, 1162
BisI GCNGC 7 cut(s) 632, 963, 1112, 1987, 2500, 3150, 3886
BlsI GCNGC 7 cut(s) 633, 964, 1113, 1988, 2501, 3151, 3887
BmcAI AGTACT 2 cut(s) 2214, 3949
Bme18I GGWCC 3 cut(s) 377, 1137, 3515
BmgT120I GGNCC 4 cut(s) 377, 654, 1137, 3515
BmiI GGNNCC 6 cut(s) 142, 655, 796, 918, 1653, 2877
BmtI GCTAGC 1 cut(s) 198
BpiI GAAGAC 2 cut(s) 2687, 3213
BplI GAGNNNNNCTC 4 cut(s) 755, 787, 2672, 2704
BpmI CTGGAG 4 cut(s) 243, 917, 1415, 1592
Bpu10I CCTNAGC 1 cut(s) 840
BpuEI CTTGAG 2 cut(s) 2343, 3254
BsaBI GATNNNNATC 2 cut(s) 945, 1146
BsaHI GRCGYC 1 cut(s) 3088
BsaI GGTCTC 1 cut(s) 1572
BsaJI CCNNGG 8 cut(s) 380, 759, 2053, 2178, 3073, 3333, 3381, 4041
BsaWI WCCGGW 1 cut(s) 391
BsaXI ACNNNNNCTCC 2 cut(s) 1296, 1326
Bsc4I CCNNNNNNNGG 8 cut(s) 485, 1065, 1807, 2349, 2556, 2885, 3073, 3585
Bse118I RCCGGY 1 cut(s) 3629
Bse21I CCTNAGG 1 cut(s) 852
Bse3DI GCAATG 3 cut(s) 1694, 2029, 3835
Bse8I GATNNNNATC 2 cut(s) 945, 1146
BseAI TCCGGA 1 cut(s) 391
BseDI CCNNGG 8 cut(s) 380, 759, 2053, 2178, 3073, 3333, 3381, 4041
BseJI GATNNNNATC 2 cut(s) 945, 1146
BseLI CCNNNNNNNGG 8 cut(s) 485, 1065, 1807, 2349, 2556, 2885, 3073, 3585
BseMI GCAATG 3 cut(s) 1694, 2029, 3835
BseRI GAGGAG 3 cut(s) 350, 819, 1786
BseSI GKGCMC 1 cut(s) 3399
BseXI GCAGC 6 cut(s) 643, 949, 1098, 2511, 3136, 3897
BseYI CCCAGC 3 cut(s) 1546, 1800, 2099
BsgI GTGCAG 1 cut(s) 706
BshFI GGCC 4 cut(s) 656, 1361, 1986, 2297
BsiHKAI GWGCWC 4 cut(s) 156, 2002, 2490, 3399
BsiSI CCGG 3 cut(s) 392, 3630, 4016
BslFI GGGAC 1 cut(s) 3184
BslI CCNNNNNNNGG 8 cut(s) 485, 1065, 1807, 2349, 2556, 2885, 3073, 3585
BsmAI GTCTC 5 cut(s) 50, 1301, 1572, 2481, 2706
BsmFI GGGAC 1 cut(s) 3184
BsmI GAATGC 2 cut(s) 2760, 2819
BsnI GGCC 4 cut(s) 656, 1361, 1986, 2297
Bso31I GGTCTC 1 cut(s) 1572
Bsp1286I GDGCHC 5 cut(s) 156, 2002, 2490, 3381, 3399
Bsp13I TCCGGA 1 cut(s) 391
Bsp1407I TGTACA 2 cut(s) 2913, 3592
Bsp19I CCATGG 1 cut(s) 3381
BspACI CCGC 6 cut(s) 926, 985, 1069, 1987, 2353, 3731
BspANI GGCC 4 cut(s) 656, 1361, 1986, 2297
BspEI TCCGGA 1 cut(s) 391
BspHI TCATGA 1 cut(s) 2611
BspLI GGNNCC 6 cut(s) 142, 655, 796, 918, 1653, 2877
BspMAI CTGCAG 4 cut(s) 967, 1116, 1563, 2484
BspMI ACCTGC 1 cut(s) 1222
BspOI GCTAGC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 3369
BspTNI GGTCTC 1 cut(s) 1572
BsrDI GCAATG 3 cut(s) 1694, 2029, 3835
BsrFI RCCGGY 1 cut(s) 3629
BsrGI TGTACA 2 cut(s) 2913, 3592
BssAI RCCGGY 1 cut(s) 3629
BssECI CCNNGG 8 cut(s) 380, 759, 2053, 2178, 3073, 3333, 3381, 4041
BssNI GRCGYC 1 cut(s) 3088
BssSI CACGAG 1 cut(s) 199
BssT1I CCWWGG 3 cut(s) 3333, 3381, 4041
Bst2BI CACGAG 1 cut(s) 199
Bst6I CTCTTC 4 cut(s) 48, 280, 3102, 3369
BstACI GRCGYC 1 cut(s) 3088
BstAPI GCANNNNNTGC 1 cut(s) 2202
BstAUI TGTACA 2 cut(s) 2913, 3592
BstC8I GCNNGC 7 cut(s) 196, 636, 1747, 2312, 2370, 3826, 4013
BstDSI CCRYGG 1 cut(s) 3381
BstEII GGTNACC 2 cut(s) 1706, 1736
BstENI CCTNNNNNAGG 2 cut(s) 483, 2883
BstHHI GCGC 1 cut(s) 1592
BstMAI GTCTC 5 cut(s) 50, 1301, 1572, 2481, 2706
BstMWI GCNNNNNNNGC 6 cut(s) 737, 2202, 2327, 2567, 2807, 3626
BstNSI RCATGY 3 cut(s) 1808, 2645, 3605
BstPI GGTNACC 2 cut(s) 1706, 1736
BstSFI CTRYAG 7 cut(s) 546, 789, 963, 1112, 1559, 2480, 2997
BstSLI GKGCMC 1 cut(s) 3399
BstV1I GCAGC 6 cut(s) 643, 949, 1098, 2511, 3136, 3897
BstV2I GAAGAC 2 cut(s) 2687, 3213
BstX2I RGATCY 8 cut(s) 115, 140, 398, 881, 916, 1162, 1249, 2875
BstXI CCANNNNNNTGG 1 cut(s) 784
BstYI RGATCY 8 cut(s) 115, 140, 398, 881, 916, 1162, 1249, 2875
Bsu36I CCTNAGG 1 cut(s) 852
BsuRI GGCC 4 cut(s) 656, 1361, 1986, 2297
BtgI CCRYGG 1 cut(s) 3381
BtsI GCAGTG 2 cut(s) 2212, 2961
BveI ACCTGC 1 cut(s) 1222
Cac8I GCNNGC 7 cut(s) 196, 636, 1747, 2312, 2370, 3826, 4013
CaiI CAGNNNCTG 3 cut(s) 1214, 1463, 2225
CciI TCATGA 1 cut(s) 2611
CfoI GCGC 1 cut(s) 1592
Cfr10I RCCGGY 1 cut(s) 3629
Cfr13I GGNCC 4 cut(s) 377, 654, 1137, 3515
CseI GACGC 1 cut(s) 3096
CsiI ACCWGGT 1 cut(s) 478
DraI TTTAAA 4 cut(s) 1864, 2271, 2584, 3969
DraIII CACNNNGTG 1 cut(s) 2597
DrdI GACNNNNNNGTC 1 cut(s) 2259
DseDI GACNNNNNNGTC 1 cut(s) 2259
Eam1104I CTCTTC 4 cut(s) 48, 280, 3102, 3369
EarI CTCTTC 4 cut(s) 48, 280, 3102, 3369
EciI GGCGGA 2 cut(s) 1000, 2368
Ecl136II GAGCTC 2 cut(s) 2000, 2488
Eco130I CCWWGG 3 cut(s) 3333, 3381, 4041
Eco147I AGGCCT 1 cut(s) 2297
Eco24I GRGCYC 3 cut(s) 2002, 2490, 3381
Eco31I GGTCTC 1 cut(s) 1572
Eco32I GATATC 2 cut(s) 2994, 3120
Eco47I GGWCC 3 cut(s) 377, 1137, 3515
Eco53kI GAGCTC 2 cut(s) 2000, 2488
Eco57I CTGAAG 6 cut(s) 2514, 2681, 3390, 3453, 3897, 3978
Eco81I CCTNAGG 1 cut(s) 852
Eco91I GGTNACC 2 cut(s) 1706, 1736
EcoICRI GAGCTC 2 cut(s) 2000, 2488
EcoNI CCTNNNNNAGG 2 cut(s) 483, 2883
EcoO65I GGTNACC 2 cut(s) 1706, 1736
EcoRV GATATC 2 cut(s) 2994, 3120
EcoT14I CCWWGG 3 cut(s) 3333, 3381, 4041
EcoT22I ATGCAT 2 cut(s) 1694, 2762
EcoT38I GRGCYC 3 cut(s) 2002, 2490, 3381
ErhI CCWWGG 3 cut(s) 3333, 3381, 4041
FalI AAGNNNNNCTT 2 cut(s) 1648, 1680
FaqI GGGAC 1 cut(s) 3184
Fnu4HI GCNGC 7 cut(s) 632, 963, 1112, 1987, 2500, 3150, 3886
FriOI GRGCYC 3 cut(s) 2002, 2490, 3381
Fsp4HI GCNGC 7 cut(s) 632, 963, 1112, 1987, 2500, 3150, 3886
FspI TGCGCA 1 cut(s) 1591
GlaI GCGC 1 cut(s) 1591
GluI GCNGC 7 cut(s) 632, 963, 1112, 1987, 2500, 3150, 3886
GsaI CCCAGC 3 cut(s) 1550, 1804, 2103
GsuI CTGGAG 4 cut(s) 243, 917, 1415, 1592
HaeIII GGCC 4 cut(s) 656, 1361, 1986, 2297
HapII CCGG 3 cut(s) 392, 3630, 4016
HgaI GACGC 1 cut(s) 3096
HhaI GCGC 1 cut(s) 1592
Hin1I GRCGYC 1 cut(s) 3088
Hin6I GCGC 1 cut(s) 1590
HinP1I GCGC 1 cut(s) 1590
HindIII AAGCTT 4 cut(s) 1062, 2857, 3137, 3223
HpaII CCGG 3 cut(s) 392, 3630, 4016
HphI GGTGA 8 cut(s) 194, 741, 1091, 1748, 1826, 2426, 2435, 3944
Hpy166II GTNNAC 5 cut(s) 1082, 2405, 2591, 2686, 3397
Hpy8I GTNNAC 5 cut(s) 1082, 2405, 2591, 2686, 3397
Hpy99I CGWCG 2 cut(s) 3072, 3090
HpyF10VI GCNNNNNNNGC 6 cut(s) 737, 2202, 2327, 2567, 2807, 3626
Hsp92I GRCGYC 1 cut(s) 3088
HspAI GCGC 1 cut(s) 1590
Kpn2I TCCGGA 1 cut(s) 391
LguI GCTCTTC 1 cut(s) 3369
LmnI GCTCC 5 cut(s) 1420, 1664, 2485, 2493, 3958
Lsp1109I GCAGC 6 cut(s) 643, 949, 1098, 2511, 3136, 3897
MabI ACCWGGT 1 cut(s) 478
MflI RGATCY 8 cut(s) 115, 140, 398, 881, 916, 1162, 1249, 2875
MhlI GDGCHC 5 cut(s) 156, 2002, 2490, 3381, 3399
MlyI GAGTC 7 cut(s) 254, 545, 662, 899, 1022, 2247, 2350
MmeI TCCRAC 4 cut(s) 355, 840, 855, 2507
Mph1103I ATGCAT 2 cut(s) 1694, 2762
MroI TCCGGA 1 cut(s) 391
MroXI GAANNNNTTC 1 cut(s) 2859
MslI CAYNNNNRTG 4 cut(s) 126, 2894, 3011, 3402
MspA1I CMGCKG 2 cut(s) 2330, 2502
MspI CCGG 3 cut(s) 392, 3630, 4016
Mva1269I GAATGC 2 cut(s) 2760, 2819
MwoI GCNNNNNNNGC 6 cut(s) 737, 2202, 2327, 2567, 2807, 3626
NcoI CCATGG 1 cut(s) 3381
NheI GCTAGC 1 cut(s) 194
NlaIV GGNNCC 6 cut(s) 142, 655, 796, 918, 1653, 2877
NmuCI GTSAC 4 cut(s) 568, 1049, 1736, 1847
NsbI TGCGCA 1 cut(s) 1591
NsiI ATGCAT 2 cut(s) 1694, 2762
NspI RCATGY 3 cut(s) 1808, 2645, 3605
PagI TCATGA 1 cut(s) 2611
PceI AGGCCT 1 cut(s) 2297
PciI ACATGT 2 cut(s) 2641, 3601
PciSI GCTCTTC 1 cut(s) 3369
PctI GAATGC 2 cut(s) 2760, 2819
PdmI GAANNNNTTC 1 cut(s) 2859
PflMI CCANNNNNTGG 2 cut(s) 1065, 1807
PfoI TCCNGGA 4 cut(s) 2342, 3253, 3310, 3774
PkrI GCNGC 7 cut(s) 633, 964, 1113, 1988, 2501, 3151, 3887
PleI GAGTC 7 cut(s) 253, 545, 662, 899, 1021, 2247, 2350
PpsI GAGTC 7 cut(s) 253, 545, 662, 899, 1021, 2247, 2350
PscI ACATGT 2 cut(s) 2641, 3601
PshBI ATTAAT 3 cut(s) 1146, 1338, 2718
PsiI TTATAA 1 cut(s) 345
Psp124BI GAGCTC 2 cut(s) 2002, 2490
PspEI GGTNACC 2 cut(s) 1706, 1736
PspFI CCCAGC 3 cut(s) 1546, 1800, 2099
PspN4I GGNNCC 6 cut(s) 142, 655, 796, 918, 1653, 2877
PspPI GGNCC 4 cut(s) 377, 654, 1137, 3515
PsrI GAACNNNNNNTAC 4 cut(s) 2622, 2654, 3307, 3339
PstI CTGCAG 4 cut(s) 967, 1116, 1563, 2484
PstNI CAGNNNCTG 3 cut(s) 1214, 1463, 2225
PsuI RGATCY 8 cut(s) 115, 140, 398, 881, 916, 1162, 1249, 2875
PvuII CAGCTG 2 cut(s) 2330, 2502
RseI CAYNNNNRTG 4 cut(s) 126, 2894, 3011, 3402
SacI GAGCTC 2 cut(s) 2002, 2490
SapI GCTCTTC 1 cut(s) 3369
SatI GCNGC 7 cut(s) 632, 963, 1112, 1987, 2500, 3150, 3886
Sau96I GGNCC 4 cut(s) 377, 654, 1137, 3515
ScaI AGTACT 2 cut(s) 2214, 3949
SchI GAGTC 7 cut(s) 254, 545, 662, 899, 1022, 2247, 2350
SduI GDGCHC 5 cut(s) 156, 2002, 2490, 3381, 3399
SexAI ACCWGGT 1 cut(s) 478
SfcI CTRYAG 7 cut(s) 546, 789, 963, 1112, 1559, 2480, 2997
SinI GGWCC 3 cut(s) 377, 1137, 3515
SmiI ATTTAAAT 1 cut(s) 2584
SmiMI CAYNNNNRTG 4 cut(s) 126, 2894, 3011, 3402
SmlI CTYRAG 2 cut(s) 2358, 3269
SmoI CTYRAG 2 cut(s) 2358, 3269
SpeI ACTAGT 1 cut(s) 4028
SseBI AGGCCT 1 cut(s) 2297
SsiI CCGC 6 cut(s) 926, 985, 1069, 1987, 2353, 3731
SspI AATATT 2 cut(s) 1474, 1678
SstI GAGCTC 2 cut(s) 2002, 2490
StuI AGGCCT 1 cut(s) 2297
StyI CCWWGG 3 cut(s) 3333, 3381, 4041
SwaI ATTTAAAT 1 cut(s) 2584
TaqI TCGA 7 cut(s) 585, 593, 1731, 1963, 2730, 3061, 3765
TatI WGTACW 5 cut(s) 2212, 2644, 2913, 3592, 3947
TauI GCSGC 1 cut(s) 1989
TseFI GTSAC 4 cut(s) 568, 1049, 1736, 1847
TseI GCWGC 6 cut(s) 631, 962, 1111, 2499, 3149, 3885
Tsp45I GTSAC 4 cut(s) 568, 1049, 1736, 1847
Van91I CCANNNNNTGG 2 cut(s) 1065, 1807
VneI GTGCAC 1 cut(s) 3395
VpaK11BI GGWCC 3 cut(s) 377, 1137, 3515
VspI ATTAAT 3 cut(s) 1146, 1338, 2718
XagI CCTNNNNNAGG 2 cut(s) 483, 2883
XapI RAATTY 5 cut(s) 1183, 2944, 3446, 3769, 3779
XbaI TCTAGA 3 cut(s) 362, 1886, 1973
XceI RCATGY 3 cut(s) 1808, 2645, 3605
XcmI CCANNNNNNNNNTGG 3 cut(s) 1808, 2644, 3160
XmnI GAANNNNTTC 1 cut(s) 2859
ZrmI AGTACT 2 cut(s) 2214, 3949
Zsp2I ATGCAT 2 cut(s) 1694, 2762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.