Rh5DG222200

presynapse organization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005139
Physical Location & Seq
Reverse (-)
24944170 .. 24957542
13373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG222200.1

Sequence Viewer

Length: 2913 bp
ATGTTTCTCAGCAAAGAAATTGAGGATCTGCATGATGATGACTTTGAGGGATCCAAAACTGAGCACCATATATTCACAGAGGTTTTCTTTGGTGGTGACGGTGCTAGCACGAGTAAGAGATGTCTTGTAACTGGAGTGATTAACTTTGAATGTGAGTCAAGTAAGACCACAGATACATCACTCTCTTCAAACAGTGAAAACTCAATTGTAACGAATCAGTCAAACTCTAAAAATACTTGTTTGGAGGAGTTTTATAACGCTACTGAGGAATCTAGAGAAATTTTTGGACCAGGGTTTTTCCCGGACAGATCCGCTTGGCTAGATACGAATGGCGAAGATGTGAGTGCTAAGAGAATGAAGTTTTCAGTTGATGAACTTCCTTGTGCAAAACCTGGTTTAGGAAAGGTAATAGATTTGTCCATAGTTTCAAAAGAAATGGTTTCTGGCTTGTCTGTTACTACAGACTCCATTACTGAAACAGTGACATTTCGTTTGGTCGAGTCTTCGAGTGAGGGAGTTACATCTAGTTGCTATCTGTTAAAGCAGCCTGCAAAACTTGACAGAGGGGCCATAGTAGGTGACTCTGACGTTTCAAAGAACAGATTACCAGCTTCTGATGGGAATGATGGAAAGGAACTATGTGCAAGTAAAGCTGTTGCTTCACCTGTTTCCCAGGAGAGCTTCTCAACCATGCTTTTGGCTACAGGTTCCCCTGTCACTCTATTGGATAAGTTGGGAACTCCGCTAGGTGCTGAGGGAAAACCCGAGGGGTTGGATAGATCTGCTATTGCCTTCAAGAAAGACTCTAGCAAGGATCCTCGTCCGCTTCTCCAGTATGATGTCAATCGCTTACTTGAAGCTGCAGGATGGCATATTCAGAGGCGGAAAAGACCTAGTAGAGCTTATATGGAGTCTGTTTATGTAACACCAAAAGGAAGGTTAATTCGTGACTTCCCCAAAGCTTGGCGGTTGTGTGGTGAACTTTTGTTTGCAGATAGGTGCAGTTCGCTGCAGAGAGATGATGCAAAGGAATGGTCCAATATTAATCAGTTTTGGTCAGATCTATCTGGTGCTTTAGTAAATTTTGAGAAAGAGATGAATCAACCAGAACCTGCTACTGAATTGGCTTATTGGTGGAGGCTCTTAGATCCCTTTGTGATTGTTGTGTTTATTGAGAGAAAGATTCTTGCTCTGAGAAAAGGAGAGACTGTTAAAGCAACTCAAAGTTTAGCTATTAATACTAATCATAAGGTTTTGGCCTTGACTAATGCGAATAGTATCAAAAATAATCTGGAGAAGGAAGATGTTTCTGCTCCCCTCTGTGATACAACTCTGGCAGGAGGGAGTGGATCGGCAGTTTCTGAGGGGAATGTTATGGAAGATGTATCGGTGGTTTGTTCTGAGGAAAGAGATGAACTGCTGGGAGGCATGACTTACGATAGGCTGGGAGATAATCTGCAGGGGTCTCTGGAGTACCAAATAAAATGCGCAAGTAATGCTGTTTTGAAAAAGAAAATAAGAAGGAAGTGTAAAAAGATTTCTGAAATGGAACCAAGCTCCACTTCTAGTAATAATATTGGTTTGCAATGCATTGATGTCAGTGGTAACCAGTCAAAGTTGAAAGAGGTCGATGGCGACCTTGCAGGCAATAAAAGAAGTAAAGGAAGTTGCAAGAAGTCCTCATCTCTCAACTCCTCCCAGCATGTGGTTGGTGAAAAAAGCCCAAAGTCTGTGAAAAATGTTCGTGAATATGATGATTTTAAAACTGGAAAGAAGAAACCATCTAGATGTGAAATCAAAGATGATGACTTACTGGTTTCAGCTATCATAAAGAACACAGATTTTAGTGCAAGTCCTGGTCGATGTTCTTCTAGAAAGAAAGGCCACAAATCTAGAGCTCATAGGAAGCATAAAAGTGAAAAGAGCCATTGCAAGTTGCTTCTTAGAAGCCTGGGTAGTGTAGGAGTAAAAACAGTTCTGTCTTGGATGCTTGATGCTGGGGTCATATTTCTGGATGATGTGATCCAGTATCGGAATACTAAGGATGGTTCTGTTATTAAAGATGGTCTAGTTACCAGGGATGGTATTTTTTGCAAGTGTTGCAGTGAAGTACTTACAGTTTCTGAGTTCAAGATTCATGGTGGGTTCAGACTAAACCGTCCATGTTTAAATCTTTTTCTGGAATCTGGTAAGGCCTTCTCCTTATGCCAGCTTCAAGCCTGGTCAGCTGAATACAAGTCCAGGAAACGGCGGACTCAAGTGGTGCGAGCTGATGAAAATGATGAAAATGATGATTCTTGCGGACTTTGTGGTGAAGGGGGCGAGCTGATATGCTGTGATAACTGCCCCTCTACTTTTCATCAGGCTTGTTTGTCTCTGCAGGAGCTTCCTGAAGGCAGCTGGTATTGTCCAAATTGCACCTGTTGGATATGTGGTCATTTTGTTACCGACAAAGGGGCTTCAAGTGCTTCTGATGGATTTAAATGTTTGCAGTGTGAGCATAAATATCATGAGGCTTGTCTGAAAGAAAAATCCATACATGTACTGGATTCTTGGTTCTGTGAAAGAAGCTGTCAAGAGGTTTACTCAGGTCTTCAGTCTCATGTTGGCTACATTAATCATGTTGTCGATGGCTTTTCATGGACACTTCTTAGATGCATTCGTGATGACCAAAAAGTTCATTCTGCCCAACGGTTATCACTGAAAGCAGAATGCAATACAAGATTAGCTGTTGCTCTTACTGTCATGGAAGAAAGCTTTCTGTCTATGGTGGATCCTAGAACAGGCATAGACATGATACCCCAAGTTTTGTACAGTTGGGGGTCAGAATTTGCTCGTTTGAATTTTCAAGGCTTCTACACTGCAGTCTTAGAGAAAGATGATGTGCTGATATCTGTAGCATCCATCAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000118 GO:0000122 GO:0000228 GO:0000785 GO:0000790 GO:0001085 GO:0001103 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003824 GO:0004402 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0005911 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006355 GO:0006357 GO:0006464 GO:0006473 GO:0006475 GO:0006725 GO:0006807 GO:0006996 GO:0008080 GO:0008134 GO:0008150 GO:0008152 GO:0009506 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010385 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0015630 GO:0016043 GO:0016070 GO:0016407 GO:0016410 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016581 GO:0016740 GO:0016746 GO:0016747 GO:0017053 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0030054 GO:0031047 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032259 GO:0032991 GO:0032993 GO:0034212 GO:0034641 GO:0036211 GO:0042393 GO:0042826 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0043414 GO:0043543 GO:0043966 GO:0043971 GO:0043972 GO:0044030 GO:0044154 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044451 GO:0044454 GO:0044464 GO:0044728 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050808 GO:0051052 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0055044 GO:0060255 GO:0061733 GO:0065007 GO:0070013 GO:0070491 GO:0070603 GO:0071704 GO:0071840 GO:0072553 GO:0080090 GO:0080188 GO:0090304 GO:0090545 GO:0090568 GO:0097159 GO:0099172 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1902679 GO:1903506 GO:1903507 GO:1904949 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas PHD

Protein Analysis

970

Amino Acids

107.31

Weight (kDa)

6.4

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 689 - 739 1e-15 Tify domain binding domain
PHD PF00628 767 - 806 6.9e-08 PHD-finger
IDM1_C PF23209 920 - 970 1.7e-15 Increased DNA methylation 1, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 255
AasI GACNNNNNNGTC 1 cut(s) 2157
Acc16I TGCGCA 1 cut(s) 1489
Acc36I ACCTGC 1 cut(s) 1120
AccB7I CCANNNNNTGG 2 cut(s) 963, 1705
AciI CCGC 7 cut(s) 312, 743, 824, 883, 967, 2251, 2301
AcsI RAATTY 4 cut(s) 279, 1081, 2828, 2842
AcuI CTGAAG 2 cut(s) 2412, 2579
AfaI GTAC 4 cut(s) 1475, 2112, 2544, 2813
AfiI CCNNNNNNNGG 6 cut(s) 398, 963, 1705, 2247, 2454, 2783
AflIII ACRYGT 1 cut(s) 2539
AjnI CCWGG 8 cut(s) 289, 391, 672, 1855, 1950, 2075, 2219, 2240
AjuI GAANNNNNNNTTGG 2 cut(s) 1546, 1578
AloI GAACNNNNNNTCC 2 cut(s) 2540, 2572
Alw21I GWGCWC 2 cut(s) 66, 1900
Alw26I GTCTC 4 cut(s) 1199, 1470, 2379, 2604
AlwNI CAGNNNCTG 4 cut(s) 614, 1112, 1361, 2123
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 4 cut(s) 567, 1257, 1882, 2193
ApeKI GCWGC 4 cut(s) 544, 860, 1009, 2397
ApoI RAATTY 4 cut(s) 279, 1081, 2828, 2842
ArsI GACNNNNNNTTYG 4 cut(s) 475, 507, 1019, 1051
AseI ATTAAT 3 cut(s) 1044, 1236, 2616
Asp700I GAANNNNTTC 1 cut(s) 2757
AspLEI GCGC 1 cut(s) 1490
AspS9I GGNCC 3 cut(s) 287, 567, 1035
AsuC2I CCSGG 1 cut(s) 302
AsuHPI GGTGA 6 cut(s) 107, 590, 654, 989, 1724, 2324
AsuNHI GCTAGC 1 cut(s) 104
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 2 cut(s) 287, 1035
BamHI GGATCC 3 cut(s) 50, 814, 2773
BanII GRGCYC 1 cut(s) 1900
BauI CACGAG 1 cut(s) 109
BbsI GAAGAC 2 cut(s) 495, 2585
Bbv12I GWGCWC 2 cut(s) 66, 1900
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 4 cut(s) 556, 847, 996, 2409
BceAI ACGGC 1 cut(s) 2264
BciT130I CCWGG 8 cut(s) 291, 393, 674, 1857, 1952, 2077, 2221, 2242
BcnI CCSGG 1 cut(s) 302
BcoDI GTCTC 4 cut(s) 1199, 1470, 2379, 2604
BfmI CTRYAG 8 cut(s) 459, 702, 861, 1010, 1457, 2378, 2862, 2895
BfuAI ACCTGC 1 cut(s) 1120
BglII AGATCT 2 cut(s) 779, 1060
BisI GCNGC 4 cut(s) 545, 861, 1010, 2398
BlsI GCNGC 4 cut(s) 546, 862, 1011, 2399
BmcAI AGTACT 1 cut(s) 2112
Bme1390I CCNGG 9 cut(s) 291, 302, 393, 674, 1857, 1952, 2077, 2221, 2242
Bme18I GGWCC 2 cut(s) 287, 1035
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 3 cut(s) 287, 567, 1035
BmiI GGNNCC 6 cut(s) 52, 568, 709, 816, 1551, 2775
BmrFI CCNGG 9 cut(s) 291, 302, 393, 674, 1857, 1952, 2077, 2221, 2242
BmsI GCATC 5 cut(s) 1012, 1977, 1984, 2645, 2909
BmtI GCTAGC 1 cut(s) 108
BpiI GAAGAC 2 cut(s) 495, 2585
BplI GAGNNNNNCTC 4 cut(s) 668, 700, 2570, 2602
BpmI CTGGAG 4 cut(s) 153, 815, 1313, 1490
Bpu10I CCTNAGC 1 cut(s) 753
BpuEI CTTGAG 1 cut(s) 2241
BpuMI CCSGG 1 cut(s) 302
BsaBI GATNNNNATC 1 cut(s) 843
BsaI GGTCTC 1 cut(s) 1470
BsaJI CCNNGG 5 cut(s) 290, 672, 765, 1951, 2076
BsaXI ACNNNNNCTCC 2 cut(s) 1194, 1224
Bsc4I CCNNNNNNNGG 6 cut(s) 398, 963, 1705, 2247, 2454, 2783
Bse1I ACTGG 7 cut(s) 136, 832, 1609, 1771, 1818, 2026, 2550
Bse3DI GCAATG 2 cut(s) 1592, 1927
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 8 cut(s) 291, 393, 674, 1857, 1952, 2077, 2221, 2242
BseDI CCNNGG 5 cut(s) 290, 672, 765, 1951, 2076
BseGI GGATG 6 cut(s) 872, 1992, 2020, 2050, 2086, 2900
BseJI GATNNNNATC 1 cut(s) 843
BseLI CCNNNNNNNGG 6 cut(s) 398, 963, 1705, 2247, 2454, 2783
BseMI GCAATG 2 cut(s) 1592, 1927
BseMII CTCAG 9 cut(s) 22, 51, 255, 744, 1184, 1353, 1392, 2115, 2601
BseNI ACTGG 7 cut(s) 136, 832, 1609, 1771, 1818, 2026, 2550
BseRI GAGGAG 2 cut(s) 260, 1684
BseXI GCAGC 4 cut(s) 556, 847, 996, 2409
BseYI CCCAGC 4 cut(s) 1420, 1444, 1698, 1997
BsgI GTGCAG 1 cut(s) 1021
BshFI GGCC 4 cut(s) 569, 1259, 1884, 2195
BsiHKAI GWGCWC 2 cut(s) 66, 1900
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 1 cut(s) 302
BslI CCNNNNNNNGG 6 cut(s) 398, 963, 1705, 2247, 2454, 2783
BsmAI GTCTC 4 cut(s) 1199, 1470, 2379, 2604
BsmI GAATGC 2 cut(s) 2658, 2717
BsnI GGCC 4 cut(s) 569, 1259, 1884, 2195
Bso31I GGTCTC 1 cut(s) 1470
BsoBI CYCGRG 1 cut(s) 764
Bsp1286I GDGCHC 2 cut(s) 66, 1900
Bsp1407I TGTACA 1 cut(s) 2811
BspACI CCGC 7 cut(s) 312, 743, 824, 883, 967, 2251, 2301
BspANI GGCC 4 cut(s) 569, 1259, 1884, 2195
BspCNI CTCAG 9 cut(s) 21, 52, 256, 745, 1185, 1354, 1393, 2116, 2600
BspHI TCATGA 1 cut(s) 2509
BspLI GGNNCC 6 cut(s) 52, 568, 709, 816, 1551, 2775
BspMAI CTGCAG 5 cut(s) 865, 1014, 1461, 2382, 2866
BspMI ACCTGC 1 cut(s) 1120
BspOI GCTAGC 1 cut(s) 108
BspTNI GGTCTC 1 cut(s) 1470
BsrDI GCAATG 2 cut(s) 1592, 1927
BsrGI TGTACA 1 cut(s) 2811
BsrI ACTGG 7 cut(s) 136, 832, 1609, 1771, 1818, 2026, 2550
BssECI CCNNGG 5 cut(s) 290, 672, 765, 1951, 2076
BssSI CACGAG 1 cut(s) 109
Bst2BI CACGAG 1 cut(s) 109
Bst2UI CCWGG 8 cut(s) 291, 393, 674, 1857, 1952, 2077, 2221, 2242
Bst6I CTCTTC 1 cut(s) 190
BstAPI GCANNNNNTGC 2 cut(s) 1496, 2100
BstAUI TGTACA 1 cut(s) 2811
BstC8I GCNNGC 6 cut(s) 106, 549, 1645, 2210, 2268, 2324
BstEII GGTNACC 1 cut(s) 1604
BstENI CCTNNNNNAGG 2 cut(s) 396, 2781
BstF5I GGATG 6 cut(s) 872, 1992, 2020, 2050, 2086, 2900
BstHHI GCGC 1 cut(s) 1490
BstMAI GTCTC 4 cut(s) 1199, 1470, 2379, 2604
BstMWI GCNNNNNNNGC 6 cut(s) 650, 1496, 2100, 2225, 2465, 2497
BstNI CCWGG 8 cut(s) 291, 393, 674, 1857, 1952, 2077, 2221, 2242
BstNSI RCATGY 2 cut(s) 1706, 2543
BstPI GGTNACC 1 cut(s) 1604
BstSCI CCNGG 9 cut(s) 289, 300, 391, 672, 1855, 1950, 2075, 2219, 2240
BstSFI CTRYAG 8 cut(s) 459, 702, 861, 1010, 1457, 2378, 2862, 2895
BstV1I GCAGC 4 cut(s) 556, 847, 996, 2409
BstV2I GAAGAC 2 cut(s) 495, 2585
BstX2I RGATCY 8 cut(s) 25, 50, 308, 779, 814, 1060, 1147, 2773
BstXI CCANNNNNNTGG 1 cut(s) 697
BstYI RGATCY 8 cut(s) 25, 50, 308, 779, 814, 1060, 1147, 2773
BsuRI GGCC 4 cut(s) 569, 1259, 1884, 2195
BtsCI GGATG 6 cut(s) 872, 1992, 2020, 2050, 2086, 2900
BtsI GCAGTG 3 cut(s) 2110, 2498, 2859
BtsIMutI CAGTG 7 cut(s) 199, 486, 1606, 2110, 2498, 2699, 2859
BveI ACCTGC 1 cut(s) 1120
Cac8I GCNNGC 6 cut(s) 106, 549, 1645, 2210, 2268, 2324
CaiI CAGNNNCTG 4 cut(s) 614, 1112, 1361, 2123
CciI TCATGA 1 cut(s) 2509
CfoI GCGC 1 cut(s) 1490
Cfr13I GGNCC 3 cut(s) 287, 567, 1035
CsiI ACCWGGT 1 cut(s) 391
Csp6I GTAC 4 cut(s) 1474, 2111, 2543, 2812
CviQI GTAC 4 cut(s) 1474, 2111, 2543, 2812
DraI TTTAAA 3 cut(s) 1762, 2169, 2482
DrdI GACNNNNNNGTC 1 cut(s) 2157
DseDI GACNNNNNNGTC 1 cut(s) 2157
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
EciI GGCGGA 2 cut(s) 898, 2266
Ecl136II GAGCTC 1 cut(s) 1898
Eco147I AGGCCT 1 cut(s) 2195
Eco24I GRGCYC 1 cut(s) 1900
Eco31I GGTCTC 1 cut(s) 1470
Eco32I GATATC 1 cut(s) 2892
Eco47I GGWCC 2 cut(s) 287, 1035
Eco53kI GAGCTC 1 cut(s) 1898
Eco57I CTGAAG 2 cut(s) 2412, 2579
Eco88I CYCGRG 1 cut(s) 764
Eco91I GGTNACC 1 cut(s) 1604
EcoICRI GAGCTC 1 cut(s) 1898
EcoNI CCTNNNNNAGG 2 cut(s) 396, 2781
EcoO65I GGTNACC 1 cut(s) 1604
EcoRII CCWGG 8 cut(s) 289, 391, 672, 1855, 1950, 2075, 2219, 2240
EcoRV GATATC 1 cut(s) 2892
EcoT22I ATGCAT 2 cut(s) 1592, 2660
EcoT38I GRGCYC 1 cut(s) 1900
FalI AAGNNNNNCTT 2 cut(s) 1546, 1578
Fnu4HI GCNGC 4 cut(s) 545, 861, 1010, 2398
FokI GGATG 6 cut(s) 879, 1999, 2027, 2057, 2093, 2887
FriOI GRGCYC 1 cut(s) 1900
Fsp4HI GCNGC 4 cut(s) 545, 861, 1010, 2398
FspI TGCGCA 1 cut(s) 1489
GlaI GCGC 1 cut(s) 1489
GluI GCNGC 4 cut(s) 545, 861, 1010, 2398
GsaI CCCAGC 4 cut(s) 1424, 1448, 1702, 2001
GsuI CTGGAG 4 cut(s) 153, 815, 1313, 1490
HaeIII GGCC 4 cut(s) 569, 1259, 1884, 2195
HapII CCGG 1 cut(s) 302
HhaI GCGC 1 cut(s) 1490
Hin6I GCGC 1 cut(s) 1488
HinP1I GCGC 1 cut(s) 1488
HindIII AAGCTT 2 cut(s) 960, 2755
HpaII CCGG 1 cut(s) 302
HphI GGTGA 6 cut(s) 107, 590, 654, 989, 1724, 2324
Hpy166II GTNNAC 2 cut(s) 980, 2584
Hpy8I GTNNAC 2 cut(s) 980, 2584
HpyAV CCTTC 7 cut(s) 802, 930, 1291, 1515, 2206, 2309, 2387
HpyCH4IV ACGT 1 cut(s) 588
HpyF10VI GCNNNNNNNGC 6 cut(s) 650, 1496, 2100, 2225, 2465, 2497
HpySE526I ACGT 1 cut(s) 588
HspAI GCGC 1 cut(s) 1488
LmnI GCTCC 3 cut(s) 1318, 1562, 2383
Lsp1109I GCAGC 4 cut(s) 556, 847, 996, 2409
LweI GCATC 5 cut(s) 1012, 1977, 1984, 2645, 2909
MabI ACCWGGT 1 cut(s) 391
MaeII ACGT 1 cut(s) 588
MboII GAAGA 9 cut(s) 177, 347, 495, 1313, 1391, 1786, 1860, 2585, 2762
MfeI CAATTG 1 cut(s) 204
MflI RGATCY 8 cut(s) 25, 50, 308, 779, 814, 1060, 1147, 2773
MhlI GDGCHC 2 cut(s) 66, 1900
MluCI AATT 9 cut(s) 18, 204, 279, 942, 1081, 1121, 2413, 2828, 2842
MlyI GAGTC 7 cut(s) 164, 458, 509, 575, 797, 920, 2248
MmeI TCCRAC 2 cut(s) 753, 2405
Mph1103I ATGCAT 2 cut(s) 1592, 2660
MroXI GAANNNNTTC 1 cut(s) 2757
MslI CAYNNNNRTG 4 cut(s) 36, 1786, 1914, 2792
MspA1I CMGCKG 2 cut(s) 2228, 2400
MspI CCGG 1 cut(s) 302
MspR9I CCNGG 9 cut(s) 291, 302, 393, 674, 1857, 1952, 2077, 2221, 2242
MunI CAATTG 1 cut(s) 204
Mva1269I GAATGC 2 cut(s) 2658, 2717
MvaI CCWGG 8 cut(s) 291, 393, 674, 1857, 1952, 2077, 2221, 2242
MwoI GCNNNNNNNGC 6 cut(s) 650, 1496, 2100, 2225, 2465, 2497
NciI CCSGG 1 cut(s) 302
NheI GCTAGC 1 cut(s) 104
NlaIV GGNNCC 6 cut(s) 52, 568, 709, 816, 1551, 2775
NmuCI GTSAC 5 cut(s) 95, 481, 578, 715, 947
NsbI TGCGCA 1 cut(s) 1489
NsiI ATGCAT 2 cut(s) 1592, 2660
NspI RCATGY 2 cut(s) 1706, 2543
PagI TCATGA 1 cut(s) 2509
PceI AGGCCT 1 cut(s) 2195
PciI ACATGT 1 cut(s) 2539
PctI GAATGC 2 cut(s) 2658, 2717
PdmI GAANNNNTTC 1 cut(s) 2757
PfeI GAWTC 8 cut(s) 214, 269, 1099, 1183, 2134, 2183, 2294, 2549
PflMI CCANNNNNTGG 2 cut(s) 963, 1705
PfoI TCCNGGA 2 cut(s) 300, 2240
PkrI GCNGC 4 cut(s) 546, 862, 1011, 2399
PleI GAGTC 7 cut(s) 163, 458, 508, 575, 797, 919, 2248
PpsI GAGTC 7 cut(s) 163, 458, 508, 575, 797, 919, 2248
PscI ACATGT 1 cut(s) 2539
PshBI ATTAAT 3 cut(s) 1044, 1236, 2616
PsiI TTATAA 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 1900
Psp6I CCWGG 8 cut(s) 289, 391, 672, 1855, 1950, 2075, 2219, 2240
PspEI GGTNACC 1 cut(s) 1604
PspFI CCCAGC 4 cut(s) 1420, 1444, 1698, 1997
PspGI CCWGG 8 cut(s) 289, 391, 672, 1855, 1950, 2075, 2219, 2240
PspN4I GGNNCC 6 cut(s) 52, 568, 709, 816, 1551, 2775
PspPI GGNCC 3 cut(s) 287, 567, 1035
PsrI GAACNNNNNNTAC 2 cut(s) 1959, 1991
PstI CTGCAG 5 cut(s) 865, 1014, 1461, 2382, 2866
PstNI CAGNNNCTG 4 cut(s) 614, 1112, 1361, 2123
PsuI RGATCY 8 cut(s) 25, 50, 308, 779, 814, 1060, 1147, 2773
PvuII CAGCTG 2 cut(s) 2228, 2400
RsaI GTAC 4 cut(s) 1475, 2112, 2544, 2813
RsaNI GTAC 4 cut(s) 1474, 2111, 2543, 2812
RseI CAYNNNNRTG 4 cut(s) 36, 1786, 1914, 2792
SacI GAGCTC 1 cut(s) 1900
SatI GCNGC 4 cut(s) 545, 861, 1010, 2398
Sau96I GGNCC 3 cut(s) 287, 567, 1035
ScaI AGTACT 1 cut(s) 2112
SchI GAGTC 7 cut(s) 164, 458, 509, 575, 797, 920, 2248
ScrFI CCNGG 9 cut(s) 291, 302, 393, 674, 1857, 1952, 2077, 2221, 2242
SduI GDGCHC 2 cut(s) 66, 1900
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 5 cut(s) 1012, 1977, 1984, 2645, 2909
SfcI CTRYAG 8 cut(s) 459, 702, 861, 1010, 1457, 2378, 2862, 2895
SinI GGWCC 2 cut(s) 287, 1035
SmiI ATTTAAAT 1 cut(s) 2482
SmiMI CAYNNNNRTG 4 cut(s) 36, 1786, 1914, 2792
SmlI CTYRAG 1 cut(s) 2256
SmoI CTYRAG 1 cut(s) 2256
Sse9I AATT 9 cut(s) 18, 204, 279, 942, 1081, 1121, 2413, 2828, 2842
SseBI AGGCCT 1 cut(s) 2195
SsiI CCGC 7 cut(s) 312, 743, 824, 883, 967, 2251, 2301
SspI AATATT 2 cut(s) 1042, 1576
SstI GAGCTC 1 cut(s) 1900
StuI AGGCCT 1 cut(s) 2195
StyD4I CCNGG 9 cut(s) 289, 300, 391, 672, 1855, 1950, 2075, 2219, 2240
SwaI ATTTAAAT 1 cut(s) 2482
TaiI ACGT 1 cut(s) 591
TaqI TCGA 5 cut(s) 498, 506, 1629, 1861, 2628
TasI AATT 9 cut(s) 18, 204, 279, 942, 1081, 1121, 2413, 2828, 2842
TatI WGTACW 3 cut(s) 2110, 2542, 2811
TfiI GAWTC 8 cut(s) 214, 269, 1099, 1183, 2134, 2183, 2294, 2549
TscAI CASTG 7 cut(s) 199, 486, 1606, 2110, 2498, 2706, 2866
TseFI GTSAC 5 cut(s) 95, 481, 578, 715, 947
TseI GCWGC 4 cut(s) 544, 860, 1009, 2397
Tsp45I GTSAC 5 cut(s) 95, 481, 578, 715, 947
TspRI CASTG 7 cut(s) 199, 486, 1606, 2110, 2498, 2706, 2866
Van91I CCANNNNNTGG 2 cut(s) 963, 1705
VpaK11BI GGWCC 2 cut(s) 287, 1035
VspI ATTAAT 3 cut(s) 1044, 1236, 2616
XagI CCTNNNNNAGG 2 cut(s) 396, 2781
XapI RAATTY 4 cut(s) 279, 1081, 2828, 2842
XbaI TCTAGA 4 cut(s) 272, 1784, 1871, 1892
XceI RCATGY 2 cut(s) 1706, 2543
XcmI CCANNNNNNNNNTGG 2 cut(s) 1706, 2542
XmnI GAANNNNTTC 1 cut(s) 2757
ZrmI AGTACT 1 cut(s) 2112
Zsp2I ATGCAT 2 cut(s) 1592, 2660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.