Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002853

Physical Location & Seq

Genomic Coordinates Reverse (-)

32742113 .. 32743973

Gene Structure 1861 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1242900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGGCCTCTCCGTTGGCCCTCCTCCTCCTCCTCCTCCTCCTCCACCAACCACTCTTTTCCGCCGCCAATGTCCACAAAATTAACCTTCTCCGATCGGAGCTCCTCTCCCAACCCACCCCACAAAACGCTGCCGCACCAACTCTCTACTTCGAAATAACCAAACCCATAGAACTCCCCAAAACCAAGCCCTGCACCCAGCACGTTCTCCAGCACGACTTCGGGTACACATACGGCAAACCTCCAGTCTTTGCTAACTACACCCCTCCCTCCTACTGCCCATCTCATTCATTTTCCAAAATTGTGCTCGAGTGGAAAGCCACCTGCAAAGGGACGCAATTTGATCGGATTTTCGGGGTTTGGCTCGGCGGCATTGAGCTTCTCAGGGGCTGCACGGCTGAGCCAACGCAGAATGGGATTGTTTGGAGTGTCAAGAAGGACATCACAAGGTACTATTCTTTGCTTCAGAACAATCAAACGCTAGCTGTTTATCTTGGGAATTTGGTAAATGAAAGATATACTGGGATTTACCATGTGAATATTAGCATCCATTTTTACCCTGCTGAGAAGAATTTGAATTATAACTATGAGCAAAATTTGGGAAATTTGGATTCTGGGTACCATCCTTGGGCGGATTTGATCTTGCCCATTTCGAGAAATCTGCCTTTCAATGATGGGTTGTGGTTTGAAATCCAGAATTCGACCGATACACAGTTGAAGGAATTTAAGATTCCACAGAATGCTTATAAGGCTGTGTTGGAGGTGTATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCAAATCCTCCGAATGAGTACATTGCTGCAAATAATCTTAGCGGTACGCCTGGAAACGGGCCTTTTAGGGAGGTTGTAGTGAGTTTAGATGGTGAGGTTGTTGGTGCAGTCTGGCCTTTTACTGTGATTTTCACTGGAGGGTTCAATCCCCTCCTATGGAGTCCAATTACTGCAATTGGCTCGTACAATCTCCCTTCTTATGATATTGAAATCACGCCCTTTATGGGGAAGATATTGGATGGGAAGAGCCACAAGTTTGGTTTTAGTGTTACAAATGCCTTAAATGTTTGGCTTGTTGATGCGAATTTGCATCTTTGGTTGGACAAGCAGAGCACGAAAACTAAAGGAGAGCTTTTGAAGAATAGTAGCTTGCCCCTTGTTGTTTCATTGGTTTCGGATTTCAAGGGTTTAAATGGCACATTTTTTACAAGGGCCGGCAGGTCTGTGTCATCAACTGGATGGGTGAAGTCCTCCTATGGGAATATCACAACTGATGCGATTCAAGACCTCCATTACAGTAATACAATGGTCATGAAGAATGATGGCAATACGCAGATAGTGAATCAGACCATCCATTCCAATGATACAGTTCATATCAGGCTGTCATCCTCCATTATCCACTCAATGTCATCAAACAAAAAATTTCCTCTTTACTTGTACATTGACTTCCTGGAACAAGCAAAAGAAACTTATAAGTACATTACAAATGTCGAACTGGGATTTATCGAGAAGAAGTCTGCAAGTGCTGGTTCCCGATTCTCCAACAGCTCTCTCAGCAATCTGCAGAATGCCGAGGGTACTATCATTATAAAAAACAGTTCCGTAGTTAGAGGACTGGGGAGTACACAGCAAGTGTATAGATTCGATGGTGGCAAATCCTGTTACTTCAGAAACATAAGCAGCTCAGACGACACCATACTCTACGATAAGGTGGGGTTTACGTGCACAAAAAAACCAGAGTCTAATGTGGATTTTGGCTTAAGCAGACTCCGGCCTTTGGGTGCTCAAAGGAGCTCTCCTGGTCCCCGATTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PNGaseA

Protein Analysis

612

Amino Acids

68.38

Weight (kDa)

8.45

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PNGaseA	PF12222	61 - 387	6.8e-104	Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C	PF25156	406 - 483	3.2e-21	PNGase_A C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14920
fragaria_vesca	FvH4_3g18311 FvH4_3g18320 FvH4_3g18320
malus_domestica	MD03G1242900.v1.1 MD03G1243300.v1.1 MD03G1243400.v1.1
prunus_persica	Prupe.4G164900_v2.0.a1
pyrus_communis	pycom11g23210
rosa_chinensis	RchiOBHm_Chr5g0030951 RchiOBHm_Chr5g0030961 RchiOBHm_Chr5g0030971
rosa_laevigata	RLG00000033274 RLG00000033275 RLG00000033276
rosa_multiflora	Rmu_sc0001974.1_g000004 Rmu_sc0001974.1_g000008 Rmu_sc0001974.1_g000009 Rmu_sc0040817.1_g000002
rosa_roxburghii	Rroxscaffold_1G00049310 Rroxscaffold_1G00049320
rosa_rugosa	Rorug05G0121600 Rorug05G0121700 Rorug05G0121900
rosa_samantha	Rh5AG214900 Rh5AG215000 Rh5AG215200 Rh5BG213900 Rh5BG214000 Rh5BG214200 Rh5CG237800 Rh5CG237900 Rh5CG238100 Rh5DG218500 Rh5DG218600 Rh5DG218800
rosa_wichuraiana	Rw5G019660 Rw5G019670 Rw5G019680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	579, 744, 1491, 1607
AarI	CACCTGC	1 cut(s)	329
Acc36I	ACCTGC	2 cut(s)	329, 1227
Acc65I	GGTACC	1 cut(s)	615
AccB1I	GGYRCC	1 cut(s)	615
AciI	CCGC	6 cut(s)	60, 63, 132, 366, 629, 840
AcsI	RAATTY	8 cut(s)	496, 568, 592, 601, 694, 719, 1102, 1439
AcuI	CTGAAG	2 cut(s)	446, 1669
AfiI	CCNNNNNNNGG	8 cut(s)	327, 625, 854, 954, 1022, 1023, 1275, 1795
AflII	CTTAAG	1 cut(s)	1777
AjnI	CCWGG	3 cut(s)	847, 1467, 1816
AluBI	AGCT	8 cut(s)	100, 376, 482, 1150, 1167, 1566, 1701, 1812
AluI	AGCT	8 cut(s)	100, 376, 482, 1150, 1167, 1566, 1701, 1812
Alw21I	GWGCWC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
Alw44I	GTGCAC	1 cut(s)	1741
AlwNI	CAGNNNCTG	1 cut(s)	387
Ama87I	CYCGRG	1 cut(s)	305
AoxI	GGCC	6 cut(s)	3, 15, 857, 911, 1230, 1790
ApaLI	GTGCAC	1 cut(s)	1741
ApeKI	GCWGC	4 cut(s)	128, 387, 824, 1698
ApoI	RAATTY	8 cut(s)	496, 568, 592, 601, 694, 719, 1102, 1439
ArsI	GACNNNNNNTTYG	2 cut(s)	228, 260
Asp718I	GGTACC	1 cut(s)	615
AspS9I	GGNCC	4 cut(s)	16, 857, 1230, 1820
AsuHPI	GGTGA	2 cut(s)	902, 1273
AsuII	TTCGAA	1 cut(s)	150
AsuNHI	GCTAGC	1 cut(s)	478
AvaI	CYCGRG	1 cut(s)	305
AvaII	GGWCC	1 cut(s)	1820
BaeGI	GKGCMC	1 cut(s)	1745
BanI	GGYRCC	1 cut(s)	615
BanII	GRGCYC	2 cut(s)	102, 1814
BarI	GAAGNNNNNNTAC	2 cut(s)	1448, 1480
Bbv12I	GWGCWC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
BbvI	GCAGC	4 cut(s)	115, 374, 811, 1710
BccI	CCATC	9 cut(s)	286, 627, 665, 881, 1031, 1251, 1334, 1376, 1658
BceAI	ACGGC	2 cut(s)	247, 408
BcgI	CGANNNNNNTGC	6 cut(s)	323, 357, 640, 674, 960, 994
BciT130I	CCWGG	3 cut(s)	849, 1469, 1818
BfaI	CTAG	1 cut(s)	479
BfmI	CTRYAG	1 cut(s)	1580
BfrI	CTTAAG	1 cut(s)	1777
BfuAI	ACCTGC	2 cut(s)	329, 1227
BisI	GCNGC	7 cut(s)	63, 129, 132, 367, 388, 825, 1699
BlpI	GCTNAGC	1 cut(s)	396
BlsI	GCNGC	7 cut(s)	64, 130, 133, 368, 389, 826, 1700
Bme1390I	CCNGG	3 cut(s)	849, 1469, 1818
Bme18I	GGWCC	1 cut(s)	1820
BmeT110I	CYCGRG	1 cut(s)	305
BmgT120I	GGNCC	4 cut(s)	16, 857, 1230, 1820
BmiI	GGNNCC	3 cut(s)	617, 1549, 1822
BmrFI	CCNGG	3 cut(s)	849, 1469, 1818
BmrI	ACTGGG	3 cut(s)	528, 1523, 1643
BmsI	GCATC	4 cut(s)	552, 1087, 1117, 1282
BmtI	GCTAGC	1 cut(s)	482
BmuI	ACTGGG	3 cut(s)	528, 1523, 1643
BplI	GAGNNNNNCTC	2 cut(s)	89, 121
BpmI	CTGGAG	3 cut(s)	191, 225, 954
Bpu1102I	GCTNAGC	1 cut(s)	396
Bpu14I	TTCGAA	1 cut(s)	150
BsaAI	YACGTR	1 cut(s)	1740
BsaJI	CCNNGG	2 cut(s)	623, 1590
Bsc4I	CCNNNNNNNGG	8 cut(s)	327, 625, 854, 954, 1022, 1023, 1275, 1795
Bse118I	RCCGGY	1 cut(s)	1232
Bse1I	ACTGG	6 cut(s)	242, 523, 937, 1258, 1518, 1638
Bse3DI	GCAATG	1 cut(s)	819
BseBI	CCWGG	3 cut(s)	849, 1469, 1818
BseDI	CCNNGG	2 cut(s)	623, 1590
BseGI	GGATG	6 cut(s)	543, 619, 1042, 1262, 1368, 1403
BseLI	CCNNNNNNNGG	8 cut(s)	327, 625, 854, 954, 1022, 1023, 1275, 1795
BseMI	GCAATG	1 cut(s)	819
BseMII	CTCAG	5 cut(s)	387, 394, 552, 1585, 1716
BseNI	ACTGG	6 cut(s)	242, 523, 937, 1258, 1518, 1638
BseRI	GAGGAG	8 cut(s)	11, 14, 17, 20, 23, 26, 29, 92
BseSI	GKGCMC	1 cut(s)	1745
BseXI	GCAGC	4 cut(s)	115, 374, 811, 1710
BseYI	CCCAGC	1 cut(s)	195
BsgI	GTGCAG	3 cut(s)	175, 373, 924
Bsh1285I	CGRYCG	2 cut(s)	95, 702
BshFI	GGCC	6 cut(s)	5, 17, 859, 913, 1232, 1792
BshNI	GGYRCC	1 cut(s)	615
BsiEI	CGRYCG	2 cut(s)	95, 702
BsiHKAI	GWGCWC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
BsiHKCI	CYCGRG	1 cut(s)	305
BsiSI	CCGG	2 cut(s)	1233, 1789
BslFI	GGGAC	2 cut(s)	343, 1806
BslI	CCNNNNNNNGG	8 cut(s)	327, 625, 854, 954, 1022, 1023, 1275, 1795
BsmFI	GGGAC	2 cut(s)	343, 1806
BsmI	GAATGC	2 cut(s)	742, 1591
BsnI	GGCC	6 cut(s)	5, 17, 859, 913, 1232, 1792
BsoBI	CYCGRG	1 cut(s)	305
Bsp119I	TTCGAA	1 cut(s)	150
Bsp1286I	GDGCHC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
Bsp1407I	TGTACA	1 cut(s)	1455
Bsp143I	GATC	3 cut(s)	92, 340, 636
Bsp1720I	GCTNAGC	1 cut(s)	396
BspACI	CCGC	6 cut(s)	60, 63, 132, 366, 629, 840
BspANI	GGCC	6 cut(s)	5, 17, 859, 913, 1232, 1792
BspCNI	CTCAG	5 cut(s)	388, 393, 553, 1584, 1715
BspHI	TCATGA	2 cut(s)	775, 1329
BspLI	GGNNCC	3 cut(s)	617, 1549, 1822
BspMAI	CTGCAG	1 cut(s)	1584
BspMI	ACCTGC	2 cut(s)	329, 1227
BspOI	GCTAGC	1 cut(s)	482
BspQI	GCTCTTC	1 cut(s)	1037
BspT104I	TTCGAA	1 cut(s)	150
BspT107I	GGYRCC	1 cut(s)	615
BspTI	CTTAAG	1 cut(s)	1777
BsrDI	GCAATG	1 cut(s)	819
BsrFI	RCCGGY	1 cut(s)	1232
BsrGI	TGTACA	1 cut(s)	1455
BsrI	ACTGG	6 cut(s)	242, 523, 937, 1258, 1518, 1638
BssAI	RCCGGY	1 cut(s)	1232
BssECI	CCNNGG	2 cut(s)	623, 1590
BssMI	GATC	3 cut(s)	92, 340, 636
BssT1I	CCWWGG	1 cut(s)	623
Bst2UI	CCWGG	3 cut(s)	849, 1469, 1818
Bst4CI	ACNGT	5 cut(s)	711, 922, 1316, 1387, 1616
Bst6I	CTCTTC	1 cut(s)	1037
BstAFI	CTTAAG	1 cut(s)	1777
BstAUI	TGTACA	1 cut(s)	1455
BstBAI	YACGTR	1 cut(s)	1740
BstBI	TTCGAA	1 cut(s)	150
BstC8I	GCNNGC	3 cut(s)	480, 1169, 1234
BstDEI	CTNAG	6 cut(s)	380, 396, 561, 836, 1571, 1702
BstF5I	GGATG	6 cut(s)	543, 619, 1042, 1262, 1368, 1403
BstKTI	GATC	3 cut(s)	95, 343, 639
BstMBI	GATC	3 cut(s)	92, 340, 636
BstMCI	CGRYCG	2 cut(s)	95, 702
BstMWI	GCNNNNNNNGC	2 cut(s)	746, 1572
BstNI	CCWGG	3 cut(s)	849, 1469, 1818
BstSCI	CCNGG	3 cut(s)	847, 1467, 1816
BstSFI	CTRYAG	1 cut(s)	1580
BstSLI	GKGCMC	1 cut(s)	1745
BstV1I	GCAGC	4 cut(s)	115, 374, 811, 1710
BstXI	CCANNNNNNTGG	1 cut(s)	1055
BsuRI	GGCC	6 cut(s)	5, 17, 859, 913, 1232, 1792
BtsCI	GGATG	6 cut(s)	543, 619, 1042, 1262, 1368, 1403
BtsIMutI	CAGTG	1 cut(s)	930
BveI	ACCTGC	2 cut(s)	329, 1227
Cac8I	GCNNGC	3 cut(s)	480, 1169, 1234
CaiI	CAGNNNCTG	1 cut(s)	387
CciI	TCATGA	2 cut(s)	775, 1329
Cfr10I	RCCGGY	1 cut(s)	1232
Cfr13I	GGNCC	4 cut(s)	16, 857, 1230, 1820
CseI	GACGC	1 cut(s)	340
CviAII	CATG	3 cut(s)	530, 776, 1330
DdeI	CTNAG	6 cut(s)	380, 396, 561, 836, 1571, 1702
DpnI	GATC	3 cut(s)	94, 342, 638
DpnII	GATC	3 cut(s)	92, 340, 636
DraI	TTTAAA	1 cut(s)	1209
Eam1104I	CTCTTC	1 cut(s)	1037
EarI	CTCTTC	1 cut(s)	1037
EciI	GGCGGA	2 cut(s)	49, 644
Ecl136II	GAGCTC	2 cut(s)	100, 1812
Eco130I	CCWWGG	1 cut(s)	623
Eco24I	GRGCYC	2 cut(s)	102, 1814
Eco47I	GGWCC	1 cut(s)	1820
Eco53kI	GAGCTC	2 cut(s)	100, 1812
Eco57I	CTGAAG	2 cut(s)	446, 1669
Eco88I	CYCGRG	1 cut(s)	305
EcoICRI	GAGCTC	2 cut(s)	100, 1812
EcoRI	GAATTC	1 cut(s)	694
EcoRII	CCWGG	3 cut(s)	847, 1467, 1816
EcoT14I	CCWWGG	1 cut(s)	623
EcoT38I	GRGCYC	2 cut(s)	102, 1814
ErhI	CCWWGG	1 cut(s)	623
FaeI	CATG	3 cut(s)	533, 779, 1333
FalI	AAGNNNNNCTT	2 cut(s)	1134, 1166
FaqI	GGGAC	2 cut(s)	343, 1806
FatI	CATG	3 cut(s)	529, 775, 1329
Fnu4HI	GCNGC	7 cut(s)	63, 129, 132, 367, 388, 825, 1699
FokI	GGATG	6 cut(s)	530, 606, 1049, 1269, 1355, 1390
FriOI	GRGCYC	2 cut(s)	102, 1814
Fsp4HI	GCNGC	7 cut(s)	63, 129, 132, 367, 388, 825, 1699
FspBI	CTAG	1 cut(s)	479
GluI	GCNGC	7 cut(s)	63, 129, 132, 367, 388, 825, 1699
GsaI	CCCAGC	1 cut(s)	199
GsuI	CTGGAG	3 cut(s)	191, 225, 954
HaeIII	GGCC	6 cut(s)	5, 17, 859, 913, 1232, 1792
HapII	CCGG	2 cut(s)	1233, 1789
HgaI	GACGC	1 cut(s)	340
Hin1II	CATG	3 cut(s)	533, 779, 1333
HinfI	GANTC	9 cut(s)	608, 727, 958, 1297, 1360, 1554, 1659, 1757, 1785
HpaII	CCGG	2 cut(s)	1233, 1789
HphI	GGTGA	2 cut(s)	902, 1273
Hpy166II	GTNNAC	5 cut(s)	73, 225, 1643, 1737, 1743
Hpy188I	TCNGA	9 cut(s)	92, 97, 345, 465, 810, 1195, 1365, 1688, 1705
Hpy188III	TCNNGA	8 cut(s)	430, 651, 691, 776, 1301, 1330, 1525, 1551
Hpy8I	GTNNAC	5 cut(s)	73, 225, 1643, 1737, 1743
HpyAV	CCTTC	4 cut(s)	95, 427, 709, 1002
HpyCH4III	ACNGT	5 cut(s)	711, 922, 1316, 1387, 1616
HpyCH4IV	ACGT	2 cut(s)	201, 1739
HpyF10VI	GCNNNNNNNGC	2 cut(s)	746, 1572
HpyF3I	CTNAG	6 cut(s)	380, 396, 561, 836, 1571, 1702
HpySE526I	ACGT	2 cut(s)	201, 1739
Hsp92II	CATG	3 cut(s)	533, 779, 1333
KpnI	GGTACC	1 cut(s)	619
KroI	GCCGGC	1 cut(s)	1232
KroNI	GCCGGC	1 cut(s)	1234
Kzo9I	GATC	3 cut(s)	92, 340, 636
LguI	GCTCTTC	1 cut(s)	1037
LmnI	GCTCC	3 cut(s)	97, 105, 1809
Lsp1109I	GCAGC	4 cut(s)	115, 374, 811, 1710
LweI	GCATC	4 cut(s)	552, 1087, 1117, 1282
MaeI	CTAG	1 cut(s)	479
MaeII	ACGT	2 cut(s)	201, 1739
MaeIII	GTNAC	2 cut(s)	1066, 1679
MalI	GATC	3 cut(s)	94, 342, 638
MboI	GATC	3 cut(s)	92, 340, 636
MboII	GAAGA	6 cut(s)	577, 1039, 1054, 1168, 1345, 1540
MfeI	CAATTG	1 cut(s)	972
MhlI	GDGCHC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
MlyI	GAGTC	3 cut(s)	967, 1766, 1779
MmeI	TCCRAC	3 cut(s)	735, 1098, 1584
MroNI	GCCGGC	1 cut(s)	1232
MseI	TTAA	5 cut(s)	81, 723, 1079, 1208, 1778
MslI	CAYNNNNRTG	2 cut(s)	780, 1377
MspCI	CTTAAG	1 cut(s)	1777
MspI	CCGG	2 cut(s)	1233, 1789
MspR9I	CCNGG	3 cut(s)	849, 1469, 1818
MunI	CAATTG	1 cut(s)	972
Mva1269I	GAATGC	2 cut(s)	742, 1591
MvaI	CCWGG	3 cut(s)	849, 1469, 1818
MwoI	GCNNNNNNNGC	2 cut(s)	746, 1572
NaeI	GCCGGC	1 cut(s)	1234
NdeII	GATC	3 cut(s)	92, 340, 636
NgoMIV	GCCGGC	1 cut(s)	1232
NheI	GCTAGC	1 cut(s)	478
NlaIII	CATG	3 cut(s)	533, 779, 1333
NlaIV	GGNNCC	3 cut(s)	617, 1549, 1822
NmeAIII	GCCGAG	2 cut(s)	342, 1615
NspV	TTCGAA	1 cut(s)	150
PaeR7I	CTCGAG	1 cut(s)	305
PagI	TCATGA	2 cut(s)	775, 1329
PaqCI	CACCTGC	1 cut(s)	329
PciSI	GCTCTTC	1 cut(s)	1037
PctI	GAATGC	2 cut(s)	742, 1591
PdiI	GCCGGC	1 cut(s)	1234
PfeI	GAWTC	6 cut(s)	608, 727, 1297, 1360, 1554, 1659
PfoI	TCCNGGA	1 cut(s)	1467
PkrI	GCNGC	7 cut(s)	64, 130, 133, 368, 389, 826, 1700
Ple19I	CGATCG	1 cut(s)	95
PleI	GAGTC	3 cut(s)	966, 1765, 1779
PpsI	GAGTC	3 cut(s)	966, 1765, 1779
Ppu21I	YACGTR	1 cut(s)	1740
PsiI	TTATAA	4 cut(s)	579, 744, 1491, 1607
Psp124BI	GAGCTC	2 cut(s)	102, 1814
Psp6I	CCWGG	3 cut(s)	847, 1467, 1816
PspFI	CCCAGC	1 cut(s)	195
PspGI	CCWGG	3 cut(s)	847, 1467, 1816
PspN4I	GGNNCC	3 cut(s)	617, 1549, 1822
PspPI	GGNCC	4 cut(s)	16, 857, 1230, 1820
PspXI	VCTCGAGB	1 cut(s)	305
PstI	CTGCAG	1 cut(s)	1584
PstNI	CAGNNNCTG	1 cut(s)	387
PvuI	CGATCG	1 cut(s)	95
RseI	CAYNNNNRTG	2 cut(s)	780, 1377
SacI	GAGCTC	2 cut(s)	102, 1814
SapI	GCTCTTC	1 cut(s)	1037
SaqAI	TTAA	5 cut(s)	81, 723, 1079, 1208, 1778
SatI	GCNGC	7 cut(s)	63, 129, 132, 367, 388, 825, 1699
Sau3AI	GATC	3 cut(s)	92, 340, 636
Sau96I	GGNCC	4 cut(s)	16, 857, 1230, 1820
SchI	GAGTC	3 cut(s)	967, 1766, 1779
ScrFI	CCNGG	3 cut(s)	849, 1469, 1818
SduI	GDGCHC	6 cut(s)	102, 306, 1133, 1745, 1804, 1814
SfaNI	GCATC	4 cut(s)	552, 1087, 1117, 1282
SfcI	CTRYAG	1 cut(s)	1580
Sfr274I	CTCGAG	1 cut(s)	305
SfuI	TTCGAA	1 cut(s)	150
SinI	GGWCC	1 cut(s)	1820
SlaI	CTCGAG	1 cut(s)	305
SmiMI	CAYNNNNRTG	2 cut(s)	780, 1377
SmlI	CTYRAG	2 cut(s)	305, 1777
SmoI	CTYRAG	2 cut(s)	305, 1777
SsiI	CCGC	6 cut(s)	60, 63, 132, 366, 629, 840
SspI	AATATT	1 cut(s)	538
SspMI	CTAG	1 cut(s)	479
SstI	GAGCTC	2 cut(s)	102, 1814
StyD4I	CCNGG	3 cut(s)	847, 1467, 1816
StyI	CCWWGG	1 cut(s)	623
TaaI	ACNGT	5 cut(s)	711, 922, 1316, 1387, 1616
TaiI	ACGT	2 cut(s)	204, 1742
TaqI	TCGA	7 cut(s)	150, 306, 650, 698, 1509, 1524, 1662
TaqII	GACCGA	1 cut(s)	716
TatI	WGTACW	4 cut(s)	816, 1455, 1494, 1640
TauI	GCSGC	3 cut(s)	65, 134, 369
TfiI	GAWTC	6 cut(s)	608, 727, 1297, 1360, 1554, 1659
Tru1I	TTAA	5 cut(s)	81, 723, 1079, 1208, 1778
Tru9I	TTAA	5 cut(s)	81, 723, 1079, 1208, 1778
TscAI	CASTG	1 cut(s)	937
TseI	GCWGC	4 cut(s)	128, 387, 824, 1698
TspDTI	ATGAA	7 cut(s)	276, 522, 759, 764, 1173, 1346, 1379
TspGWI	ACGGA	1 cut(s)	1609
TspRI	CASTG	1 cut(s)	937
Vha464I	CTTAAG	1 cut(s)	1777
VneI	GTGCAC	1 cut(s)	1741
VpaK11BI	GGWCC	1 cut(s)	1820
XapI	RAATTY	8 cut(s)	496, 568, 592, 601, 694, 719, 1102, 1439
XhoI	CTCGAG	1 cut(s)	305
XspI	CTAG	1 cut(s)	479

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.