RchiOBHm_Chr5g0030951

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002853
Physical Location & Seq
Forward (+)
24581113 .. 24583994
2882 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31024

Sequence Viewer

Length: 1782 bp
ATGGCTTCTCTGTTGCATCTCCTCTTCTTCCTCTTCCTTCAACCTCTCTTCTCCATTGCCAATGTCCACAAACTCAACCAACTCAGATCAGAGCTCATCTCCCAACCAACACCTCTAAATGACACCCCACCAACCCTTTACTTTGAAGTAACCAAACCCATTAAACTACCCAAAACCAAGCCTTGTTCCCAGCTCATTCTCCAGCATGACTTTGCCTACACTTATAGAAAAGCCCCAGTCTTTGCTAACTACCACCCTCCCTCAAATTGCCCATCTCAGAATTTCTCCAAGATTGTTCTTGAATGGAAAGCTACAAGCAAAGGAAGGCAATTTGACCGCATTTTTGGGGTTTGGCTCTCTGGGGTTGAGCTTCTCAGGAGCTGCACCGCAGAGCCGAGGCCTAATGGGATTGTTTGGAGTGTTGAGAAGGACATCACCAGGTACTTTTCATTACTCCAGAAGAATCAGACACTTGCTGTTTATCTTGGCAATTTGATTGATAAAACCTATACTGGGGTTTACCATGTCAATGTTAGTATTCATTTTTATCCTGCTGAAGAGAACTTGATTGATCATGACCAAAAGTTGGCATCTTGGGCGGATTTGATTCTGCCCATTTCTAGAAATTTGCCTTTGAAGGATGGGTTGTGGTTTGAAATTGAGAATTCAACTGATACCAAGTTGAAGGAATTTGAGATTCCTCGAAATGCTTATAGGGCTGTGTTGGAAGTGTATGTTTCCTTTCATGAGAATGATGAATCCTGGTATGCAAATCCTACTAATGAGTACATTGCTGCAAACAACCTTAGCGTGCCCGGAAATGGACCCTTTAGGGAGGTTGTGGTCAGTATAGATGGTCATGTTGTTGGTGCTGTGTGGCCTTTTACAGTGATATTCACAGGAGGGGTCAATCCTCTCCTGTGGCGACCCATTACTGCAATTGGCTCATACGATCTTCCTTCTTATGACATCGAAATCACACCGTTTTTAGGGAACATATTGGATGGGAAGAGCCACAAGATTGGCTTTAATGTTACAAATGCCTTGAATGTTTGGTACATAGATGCAAATTTGCATATTTGGTTGGACAAACAGAGCAGAAGAACTGAGGGACAGCTTGTGAAGCACACAAGCTTGCCCCTTGTTATTTCACTGGTGTCAGATTTCAAGGGTTTAAATGGCACATTTATCACAAGGGTGAGCAGGTCTGTCTCATCAACTGGGTGGGTGAAGTCATCGAATGGGAATATTACAACCCAGTCAATTCAAAAATTTTACTACAGTAATACAATGGTCATGGGGAATAATGGTGATGTGCAGATAGTGAACCAGAACATCCATTTCAATGACACTGTTCATATCATGTCGCCTTCCTCCATCGTTCACTCACTCTCATCACACAAAACATTTCCCCTTTACTTGTACACTGACTACTTGGAACAAAAAAATAATACATATTTATTGGTGACAAATGTCAGTCTGGGAGTTATTGAGAAGAAAACTGCAAGTGCTGGTTCTCGATTCATGAAGAGCTCTCTCAGAAATTTGCAGAATGCAGAGGGTCATATGGTTGTAAAAGATAATTTGGTAGTCAGTGGAGTCGGGAATACACAGCAAATGTACAGATATCATGGTGGAAAATTCTGTTACTTTAGGAATATAAGCAGCTCAAACTACACCATACTTTATGATAAGGTTGAGAATACATGCAACAACAAACCAAAGTCTAACTGGGATTTTGGTTTGAGAAGACTGTGGCCTTTTGGTGCTGGATTTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

593

Amino Acids

67.32

Weight (kDa)

8.93

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 59 - 376 4.1e-104 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 395 - 472 8.5e-22 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1194
AccB7I CCANNNNNTGG 1 cut(s) 586
AciI CCGC 3 cut(s) 337, 387, 599
AcsI RAATTY 8 cut(s) 280, 625, 664, 689, 1069, 1271, 1543, 1640
AcuI CTGAAG 1 cut(s) 576
AfaI GTAC 5 cut(s) 443, 788, 1058, 1424, 1622
AfiI CCNNNNNNNGG 4 cut(s) 513, 586, 821, 989
AjnI CCWGG 2 cut(s) 437, 761
AleI CACNNNNGTG 1 cut(s) 1196
AluBI AGCT 9 cut(s) 94, 193, 311, 370, 381, 1117, 1134, 1533, 1668
AluI AGCT 9 cut(s) 94, 193, 311, 370, 381, 1117, 1134, 1533, 1668
Alw21I GWGCWC 2 cut(s) 96, 1535
Alw26I GTCTC 1 cut(s) 1216
AlwNI CAGNNNCTG 1 cut(s) 381
AoxI GGCC 3 cut(s) 398, 878, 1757
ApeKI GCWGC 3 cut(s) 381, 794, 1665
ApoI RAATTY 8 cut(s) 280, 625, 664, 689, 1069, 1271, 1543, 1640
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspS9I GGNCC 1 cut(s) 824
AsuC2I CCSGG 1 cut(s) 816
AsuHPI GGTGA 5 cut(s) 427, 1210, 1240, 1322, 1477
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 816
BanII GRGCYC 2 cut(s) 96, 1535
BbsI GAAGAC 1 cut(s) 1756
Bbv12I GWGCWC 2 cut(s) 96, 1535
BbvI GCAGC 3 cut(s) 368, 781, 1677
BccI CCATC 5 cut(s) 280, 635, 848, 998, 1385
BciT130I CCWGG 2 cut(s) 439, 763
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 1 cut(s) 816
BcoDI GTCTC 1 cut(s) 1216
BfaI CTAG 1 cut(s) 621
BfmI CTRYAG 1 cut(s) 1279
BfuAI ACCTGC 1 cut(s) 1194
BisI GCNGC 3 cut(s) 382, 795, 1666
BlsI GCNGC 3 cut(s) 383, 796, 1667
Bme1390I CCNGG 3 cut(s) 439, 763, 816
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 1 cut(s) 826
BmrFI CCNGG 3 cut(s) 439, 763, 816
BmrI ACTGGG 5 cut(s) 230, 522, 1230, 1252, 1741
BmsI GCATC 3 cut(s) 25, 599, 1054
BmuI ACTGGG 5 cut(s) 230, 522, 1230, 1252, 1741
BoxI GACNNNNGTC 1 cut(s) 1472
BpiI GAAGAC 1 cut(s) 1756
BplI GAGNNNNNCTC 2 cut(s) 83, 115
BpmI CTGGAG 2 cut(s) 185, 440
Bpu10I CCTNAGC 1 cut(s) 806
BpuMI CCSGG 1 cut(s) 816
BsaJI CCNNGG 1 cut(s) 395
Bsc4I CCNNNNNNNGG 4 cut(s) 513, 586, 821, 989
Bse1I ACTGG 6 cut(s) 236, 517, 1158, 1225, 1258, 1736
Bse3DI GCAATG 2 cut(s) 54, 789
BseBI CCWGG 2 cut(s) 439, 763
BseDI CCNNGG 1 cut(s) 395
BseGI GGATG 3 cut(s) 646, 1009, 1335
BseLI CCNNNNNNNGG 4 cut(s) 513, 586, 821, 989
BseMI GCAATG 2 cut(s) 54, 789
BseMII CTCAG 5 cut(s) 97, 290, 388, 1098, 1552
BseNI ACTGG 6 cut(s) 236, 517, 1158, 1225, 1258, 1736
BseRI GAGGAG 1 cut(s) 11
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 3 cut(s) 368, 781, 1677
BseYI CCCAGC 1 cut(s) 189
BsgI GTGCAG 2 cut(s) 367, 1337
BshFI GGCC 3 cut(s) 400, 880, 1759
BsiHKAI GWGCWC 2 cut(s) 96, 1535
BsiSI CCGG 1 cut(s) 816
BslFI GGGAC 1 cut(s) 1125
BslI CCNNNNNNNGG 4 cut(s) 513, 586, 821, 989
BsmAI GTCTC 1 cut(s) 1216
BsmFI GGGAC 1 cut(s) 1125
BsmI GAATGC 1 cut(s) 1558
BsnI GGCC 3 cut(s) 400, 880, 1759
Bsp1286I GDGCHC 3 cut(s) 96, 816, 1535
Bsp1407I TGTACA 2 cut(s) 1422, 1620
Bsp143I GATC 3 cut(s) 86, 571, 952
BspACI CCGC 3 cut(s) 337, 387, 599
BspANI GGCC 3 cut(s) 400, 880, 1759
BspCNI CTCAG 5 cut(s) 96, 289, 387, 1099, 1551
BspHI TCATGA 3 cut(s) 574, 745, 1524
BspLI GGNNCC 1 cut(s) 826
BspMI ACCTGC 1 cut(s) 1194
BspQI GCTCTTC 2 cut(s) 1004, 1523
BsrDI GCAATG 2 cut(s) 54, 789
BsrGI TGTACA 2 cut(s) 1422, 1620
BsrI ACTGG 6 cut(s) 236, 517, 1158, 1225, 1258, 1736
BssECI CCNNGG 1 cut(s) 395
BssMI GATC 3 cut(s) 86, 571, 952
Bst2UI CCWGG 2 cut(s) 439, 763
Bst4CI ACNGT 5 cut(s) 889, 984, 1283, 1354, 1755
Bst6I CTCTTC 6 cut(s) 29, 38, 53, 552, 1004, 1523
BstAUI TGTACA 2 cut(s) 1422, 1620
BstC8I GCNNGC 2 cut(s) 812, 1136
BstDEI CTNAG 6 cut(s) 83, 276, 374, 806, 1107, 1538
BstF5I GGATG 3 cut(s) 646, 1009, 1335
BstKTI GATC 3 cut(s) 89, 574, 955
BstMAI GTCTC 1 cut(s) 1216
BstMBI GATC 3 cut(s) 86, 571, 952
BstMWI GCNNNNNNNGC 3 cut(s) 596, 716, 1123
BstNI CCWGG 2 cut(s) 439, 763
BstNSI RCATGY 1 cut(s) 1710
BstPAI GACNNNNGTC 1 cut(s) 1472
BstSCI CCNGG 3 cut(s) 437, 761, 814
BstSFI CTRYAG 1 cut(s) 1279
BstSLI GKGCMC 1 cut(s) 816
BstV1I GCAGC 3 cut(s) 368, 781, 1677
BstV2I GAAGAC 1 cut(s) 1756
BstXI CCANNNNNNTGG 1 cut(s) 1022
BsuRI GGCC 3 cut(s) 400, 880, 1759
BtsCI GGATG 3 cut(s) 646, 1009, 1335
BtsIMutI CAGTG 5 cut(s) 894, 1151, 1350, 1425, 1600
BveI ACCTGC 1 cut(s) 1194
Cac8I GCNNGC 2 cut(s) 812, 1136
CaiI CAGNNNCTG 1 cut(s) 381
CciI TCATGA 3 cut(s) 574, 745, 1524
Cfr13I GGNCC 1 cut(s) 824
CsiI ACCWGGT 1 cut(s) 437
Csp6I GTAC 5 cut(s) 442, 787, 1057, 1423, 1621
CviQI GTAC 5 cut(s) 442, 787, 1057, 1423, 1621
DdeI CTNAG 6 cut(s) 83, 276, 374, 806, 1107, 1538
DpnI GATC 3 cut(s) 88, 573, 954
DpnII GATC 3 cut(s) 86, 571, 952
DraI TTTAAA 1 cut(s) 1176
Eam1104I CTCTTC 6 cut(s) 29, 38, 53, 552, 1004, 1523
EarI CTCTTC 6 cut(s) 29, 38, 53, 552, 1004, 1523
EciI GGCGGA 1 cut(s) 614
Ecl136II GAGCTC 2 cut(s) 94, 1533
Eco147I AGGCCT 1 cut(s) 400
Eco24I GRGCYC 2 cut(s) 96, 1535
Eco32I GATATC 1 cut(s) 1628
Eco47I GGWCC 1 cut(s) 824
Eco53kI GAGCTC 2 cut(s) 94, 1533
Eco57I CTGAAG 1 cut(s) 576
EcoICRI GAGCTC 2 cut(s) 94, 1533
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 437, 761
EcoRV GATATC 1 cut(s) 1628
EcoT38I GRGCYC 2 cut(s) 96, 1535
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FaqI GGGAC 1 cut(s) 1125
FauNDI CATATG 1 cut(s) 1566
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 3 cut(s) 382, 795, 1666
FokI GGATG 3 cut(s) 653, 1016, 1322
FriOI GRGCYC 2 cut(s) 96, 1535
Fsp4HI GCNGC 3 cut(s) 382, 795, 1666
FspBI CTAG 1 cut(s) 621
GluI GCNGC 3 cut(s) 382, 795, 1666
GsaI CCCAGC 1 cut(s) 193
GsuI CTGGAG 2 cut(s) 185, 440
HaeIII GGCC 3 cut(s) 400, 880, 1759
HapII CCGG 1 cut(s) 816
HindIII AAGCTT 1 cut(s) 1132
HinfI GANTC 6 cut(s) 463, 607, 697, 758, 1521, 1599
HpaII CCGG 1 cut(s) 816
HphI GGTGA 5 cut(s) 427, 1210, 1240, 1322, 1477
Hpy166II GTNNAC 5 cut(s) 67, 520, 1327, 1384, 1425
Hpy188I TCNGA 6 cut(s) 86, 91, 279, 468, 1162, 1541
Hpy188III TCNNGA 9 cut(s) 299, 376, 457, 575, 621, 746, 1518, 1525, 1603
Hpy8I GTNNAC 5 cut(s) 67, 520, 1327, 1384, 1425
HpyAV CCTTC 7 cut(s) 47, 318, 421, 631, 679, 969, 1380
HpyCH4III ACNGT 5 cut(s) 889, 984, 1283, 1354, 1755
HpyF10VI GCNNNNNNNGC 3 cut(s) 596, 716, 1123
HpyF3I CTNAG 6 cut(s) 83, 276, 374, 806, 1107, 1538
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 3 cut(s) 86, 571, 952
LguI GCTCTTC 2 cut(s) 1004, 1523
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 3 cut(s) 368, 781, 1677
LweI GCATC 3 cut(s) 25, 599, 1054
MabI ACCWGGT 1 cut(s) 437
MaeI CTAG 1 cut(s) 621
MaeIII GTNAC 4 cut(s) 148, 1033, 1465, 1646
MalI GATC 3 cut(s) 88, 573, 954
MboI GATC 3 cut(s) 86, 571, 952
MfeI CAATTG 1 cut(s) 939
MhlI GDGCHC 3 cut(s) 96, 816, 1535
MlyI GAGTC 1 cut(s) 1608
MmeI TCCRAC 2 cut(s) 705, 1065
MseI TTAA 3 cut(s) 162, 1029, 1175
MslI CAYNNNNRTG 4 cut(s) 528, 750, 1196, 1344
MspI CCGG 1 cut(s) 816
MspR9I CCNGG 3 cut(s) 439, 763, 816
MunI CAATTG 1 cut(s) 939
Mva1269I GAATGC 1 cut(s) 1558
MvaI CCWGG 2 cut(s) 439, 763
MwoI GCNNNNNNNGC 3 cut(s) 596, 716, 1123
NciI CCSGG 1 cut(s) 816
NdeI CATATG 1 cut(s) 1566
NdeII GATC 3 cut(s) 86, 571, 952
NlaIV GGNNCC 1 cut(s) 826
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 1 cut(s) 1465
NspI RCATGY 1 cut(s) 1710
OliI CACNNNNGTG 1 cut(s) 1196
PagI TCATGA 3 cut(s) 574, 745, 1524
PceI AGGCCT 1 cut(s) 400
PciSI GCTCTTC 2 cut(s) 1004, 1523
PctI GAATGC 1 cut(s) 1558
PfeI GAWTC 5 cut(s) 463, 607, 697, 758, 1521
PflMI CCANNNNNTGG 1 cut(s) 586
PkrI GCNGC 3 cut(s) 383, 796, 1667
PleI GAGTC 1 cut(s) 1607
PpsI GAGTC 1 cut(s) 1607
PshAI GACNNNNGTC 1 cut(s) 1472
Psp124BI GAGCTC 2 cut(s) 96, 1535
Psp6I CCWGG 2 cut(s) 437, 761
PspFI CCCAGC 1 cut(s) 189
PspGI CCWGG 2 cut(s) 437, 761
PspN4I GGNNCC 1 cut(s) 826
PspPI GGNCC 1 cut(s) 824
PstNI CAGNNNCTG 1 cut(s) 381
RsaI GTAC 5 cut(s) 443, 788, 1058, 1424, 1622
RsaNI GTAC 5 cut(s) 442, 787, 1057, 1423, 1621
RseI CAYNNNNRTG 4 cut(s) 528, 750, 1196, 1344
SacI GAGCTC 2 cut(s) 96, 1535
SapI GCTCTTC 2 cut(s) 1004, 1523
SaqAI TTAA 3 cut(s) 162, 1029, 1175
SatI GCNGC 3 cut(s) 382, 795, 1666
Sau3AI GATC 3 cut(s) 86, 571, 952
Sau96I GGNCC 1 cut(s) 824
SchI GAGTC 1 cut(s) 1608
ScrFI CCNGG 3 cut(s) 439, 763, 816
SduI GDGCHC 3 cut(s) 96, 816, 1535
SexAI ACCWGGT 1 cut(s) 437
SfaNI GCATC 3 cut(s) 25, 599, 1054
SfcI CTRYAG 1 cut(s) 1279
SinI GGWCC 1 cut(s) 824
SmiMI CAYNNNNRTG 4 cut(s) 528, 750, 1196, 1344
SseBI AGGCCT 1 cut(s) 400
SsiI CCGC 3 cut(s) 337, 387, 599
SspI AATATT 1 cut(s) 1249
SspMI CTAG 1 cut(s) 621
SstI GAGCTC 2 cut(s) 96, 1535
StuI AGGCCT 1 cut(s) 400
StyD4I CCNGG 3 cut(s) 437, 761, 814
TaaI ACNGT 5 cut(s) 889, 984, 1283, 1354, 1755
TaqI TCGA 4 cut(s) 703, 972, 1238, 1519
TatI WGTACW 3 cut(s) 786, 1422, 1620
TfiI GAWTC 5 cut(s) 463, 607, 697, 758, 1521
Tru1I TTAA 3 cut(s) 162, 1029, 1175
Tru9I TTAA 3 cut(s) 162, 1029, 1175
TscAI CASTG 5 cut(s) 894, 1158, 1357, 1432, 1600
TseFI GTSAC 1 cut(s) 1465
TseI GCWGC 3 cut(s) 381, 794, 1665
Tsp45I GTSAC 1 cut(s) 1465
TspDTI ATGAA 7 cut(s) 438, 530, 734, 771, 1346, 1513, 1541
TspRI CASTG 5 cut(s) 894, 1158, 1357, 1432, 1600
Van91I CCANNNNNTGG 1 cut(s) 586
VpaK11BI GGWCC 1 cut(s) 824
XapI RAATTY 8 cut(s) 280, 625, 664, 689, 1069, 1271, 1543, 1640
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 1 cut(s) 1710
XcmI CCANNNNNNNNNTGG 1 cut(s) 1728
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.