Rh5BG214200

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002853
Physical Location & Seq
Forward (+)
24736277 .. 24739078
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG214200.1

Sequence Viewer

Length: 1425 bp
ATGGCTACTCTATTGCCTCTCCTCTTCTTCCTCCTCCAACCCCTCTTCTCCATTGCCAATGTCCACAAACTCAACCAACTCAGATCAGAGCTCATCTCCCAATCCCCTTCTCTCAATGACACCCCACCCAGCCTTGACTTTGAAGTAACCAAACCCATTCCAGTACCCAAAACCAAGCCTTGTTCTGAGCTCATTCTCCAACATGATTTTGCCTACACTTATGGAAAACCACCAGTCTTTGCTGACTACACTCCTCCCTCAAATTGCCCGTCTCAGAAGTTCTCCAAGATTGTTCTTGAATGGAAAGCTACAAGCAAAGGAAGACAATTTGACCGCATTTTTGGGGTTTGGCTTTCTGGTGTTGAGCTTCTCAGGAGCTGCACAGCTGAGCCAAGGACCAATGGGATTGTCTGGAGTGTTCAGAAGGACATCAGCAGGTACTATTCGTTGCTCCAAAAGAAACAGACAGTTGCTGTTTATCTTGGCAGTTTGATTGATTCAACATACACTGGAGTTTACCATGTGAATATAAGTTTTCATTTTTATCCTGCTGAGGAGAGTTTGCATATTTATGATCAAAATGTGGGTGATTTGGCATCTGGATATCATTCTTGGGCGGATTTGATTCTGCCCATTTCGAGAAATCTGCCTTTGACTGATGGGTTGTGGTTTGAAGTTGAGAATTCAACTGATACTGAGTTGAAGGAATTCGAGATTCCTCGAAATGCTTATAGGGCTGTGTTGGAGGTTTATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCCAATCCTACTAATGAGTACATTGCTGCCAACAACCTTAGTGGCACGCCCGGAAATGGGCCTTTTAGGGAGGTTGTGGTGAGTTTAGATGGTGTGGTTGTTGGTGCTGTTTGGCCTTTTACTGTGATTTACACTGGAGGGTTCAATCCTCTTCTGTGGAGACCCATTACCGGCATTGGTTCATTTGATCTACCTTCTTATGATATCGAAATCACACCGTTTTTAGGGAACATATTGGATGGGAAGAGCCACAAGATTGGGTTTAATGTTACCAATGCCTTGAATGTATGGTACATCGATGCAAATTTGCATATTTGGTTGGACAAACAGGGCACAAGAACTCAAGCACAGCTTGTGAAGCATACTAGCTTGCCCCTTATTGTTTCAGTGGTGTCGGATTTTGTGGGTTTAAATGGCACATTTGTGACAAGGGTCAACAGGTCCGTCTTATCAACTGGGTGGGTGAAGTCTTCTAATGGGAATATCACAACCCAGTCAATTCAAGATTTTTCCTACATTAATAAAATTGTCATGGGGAATGATGGAAATACGCAGACAGTGGATCAGAACATAAATTTCAATGATACCGATAGGCGAGTTCTTTTAATTGAAGCTAGCTGTCCACTTGAGCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

474

Amino Acids

53.22

Weight (kDa)

5.41

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 57 - 383 1.9e-104 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 402 - 458 5.3e-15 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 426
AciI CCGC 2 cut(s) 334, 617
AclWI GGATC 1 cut(s) 1356
AcsI RAATTY 4 cut(s) 682, 707, 1090, 1360
AfaI GTAC 4 cut(s) 165, 440, 806, 1079
AfiI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
AleI CACNNNNGTG 1 cut(s) 1208
Alw21I GWGCWC 2 cut(s) 93, 192
Alw26I GTCTC 2 cut(s) 276, 940
AlwI GGATC 1 cut(s) 1356
AlwNI CAGNNNCTG 2 cut(s) 378, 473
AoxI GGCC 2 cut(s) 845, 899
ApeKI GCWGC 2 cut(s) 378, 812
ApoI RAATTY 4 cut(s) 682, 707, 1090, 1360
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 323, 355
AseI ATTAAT 1 cut(s) 1305
Asp700I GAANNNNTTC 1 cut(s) 707
AspS9I GGNCC 3 cut(s) 396, 845, 1227
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 3 cut(s) 599, 877, 1261
AsuNHI GCTAGC 1 cut(s) 1400
AvaII GGWCC 2 cut(s) 396, 1227
BaeGI GKGCMC 1 cut(s) 1121
BanII GRGCYC 2 cut(s) 93, 192
BbsI GAAGAC 2 cut(s) 328, 1248
Bbv12I GWGCWC 2 cut(s) 93, 192
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 365, 799
BccI CCATC 4 cut(s) 653, 869, 1019, 1322
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 2 cut(s) 276, 940
BfaI CTAG 2 cut(s) 1152, 1401
BfuAI ACCTGC 1 cut(s) 426
BisI GCNGC 2 cut(s) 379, 813
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 2 cut(s) 380, 814
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 2 cut(s) 396, 1227
BmgT120I GGNCC 3 cut(s) 396, 845, 1227
BmrFI CCNGG 1 cut(s) 837
BmrI ACTGGG 2 cut(s) 1251, 1273
BmsI GCATC 2 cut(s) 605, 1075
BmtI GCTAGC 1 cut(s) 1404
BmuI ACTGGG 2 cut(s) 1251, 1273
BoxI GACNNNNGTC 1 cut(s) 1217
BpiI GAAGAC 2 cut(s) 328, 1248
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpmI CTGGAG 3 cut(s) 433, 531, 942
Bpu10I CCTNAGC 1 cut(s) 552
Bpu1102I GCTNAGC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 1113
BpuMI CCSGG 1 cut(s) 837
Bsa29I ATCGAT 1 cut(s) 1083
BsaI GGTCTC 1 cut(s) 940
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 6 cut(s) 161, 233, 514, 925, 1246, 1279
Bse3DI GCAATG 2 cut(s) 51, 807
BseCI ATCGAT 1 cut(s) 1083
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 1030
BseLI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
BseMI GCAATG 2 cut(s) 51, 807
BseMII CTCAG 7 cut(s) 94, 177, 287, 378, 385, 543, 687
BseNI ACTGG 6 cut(s) 161, 233, 514, 925, 1246, 1279
BseRI GAGGAG 4 cut(s) 11, 23, 243, 569
BseSI GKGCMC 1 cut(s) 1121
BseXI GCAGC 2 cut(s) 365, 799
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 2 cut(s) 847, 901
BshVI ATCGAT 1 cut(s) 1083
BsiHKAI GWGCWC 2 cut(s) 93, 192
BsiSI CCGG 2 cut(s) 837, 957
BslI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
BsmAI GTCTC 2 cut(s) 276, 940
BsmBI CGTCTC 1 cut(s) 276
BsnI GGCC 2 cut(s) 847, 901
Bso31I GGTCTC 1 cut(s) 940
Bsp1286I GDGCHC 3 cut(s) 93, 192, 1121
Bsp143I GATC 4 cut(s) 83, 574, 973, 1348
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 2 cut(s) 334, 617
BspANI GGCC 2 cut(s) 847, 901
BspCNI CTCAG 7 cut(s) 93, 178, 286, 379, 384, 544, 688
BspDI ATCGAT 1 cut(s) 1083
BspHI TCATGA 1 cut(s) 763
BspMI ACCTGC 1 cut(s) 426
BspOI GCTAGC 1 cut(s) 1404
BspPI GGATC 1 cut(s) 1356
BspQI GCTCTTC 1 cut(s) 1025
BspTNI GGTCTC 1 cut(s) 940
BsrDI GCAATG 2 cut(s) 51, 807
BsrFI RCCGGY 1 cut(s) 956
BsrI ACTGG 6 cut(s) 161, 233, 514, 925, 1246, 1279
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 4 cut(s) 83, 574, 973, 1348
BssT1I CCWWGG 1 cut(s) 392
Bst4CI ACNGT 4 cut(s) 469, 910, 1005, 1345
Bst6I CTCTTC 4 cut(s) 29, 50, 942, 1025
BstC8I GCNNGC 3 cut(s) 833, 1157, 1402
BstDEI CTNAG 8 cut(s) 80, 186, 273, 371, 387, 552, 696, 824
BstF5I GGATG 1 cut(s) 1030
BstKTI GATC 4 cut(s) 86, 577, 976, 1351
BstMAI GTCTC 2 cut(s) 276, 940
BstMBI GATC 4 cut(s) 83, 574, 973, 1348
BstMWI GCNNNNNNNGC 2 cut(s) 734, 1144
BstPAI GACNNNNGTC 1 cut(s) 1217
BstSCI CCNGG 1 cut(s) 835
BstSLI GKGCMC 1 cut(s) 1121
BstV1I GCAGC 2 cut(s) 365, 799
BstV2I GAAGAC 2 cut(s) 328, 1248
BstXI CCANNNNNNTGG 1 cut(s) 1043
Bsu15I ATCGAT 1 cut(s) 1083
BsuRI GGCC 2 cut(s) 847, 901
BsuTUI ATCGAT 1 cut(s) 1083
BtsCI GGATG 1 cut(s) 1030
BtsIMutI CAGTG 4 cut(s) 507, 918, 1179, 1350
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 3 cut(s) 833, 1157, 1402
CaiI CAGNNNCTG 2 cut(s) 378, 473
CciI TCATGA 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 3 cut(s) 396, 845, 1227
ClaI ATCGAT 1 cut(s) 1083
Csp6I GTAC 4 cut(s) 164, 439, 805, 1078
CspCI CAANNNNNGTGG 2 cut(s) 808, 843
CviAII CATG 4 cut(s) 203, 521, 764, 1318
CviQI GTAC 4 cut(s) 164, 439, 805, 1078
DdeI CTNAG 8 cut(s) 80, 186, 273, 371, 387, 552, 696, 824
DpnI GATC 4 cut(s) 85, 576, 975, 1350
DpnII GATC 4 cut(s) 83, 574, 973, 1348
DraI TTTAAA 1 cut(s) 1197
Eam1104I CTCTTC 4 cut(s) 29, 50, 942, 1025
EarI CTCTTC 4 cut(s) 29, 50, 942, 1025
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 2 cut(s) 91, 190
Eco130I CCWWGG 1 cut(s) 392
Eco24I GRGCYC 2 cut(s) 93, 192
Eco31I GGTCTC 1 cut(s) 940
Eco32I GATATC 2 cut(s) 605, 991
Eco47I GGWCC 2 cut(s) 396, 1227
Eco53kI GAGCTC 2 cut(s) 91, 190
EcoICRI GAGCTC 2 cut(s) 91, 190
EcoRI GAATTC 2 cut(s) 682, 707
EcoRV GATATC 2 cut(s) 605, 991
EcoT14I CCWWGG 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 93, 192
ErhI CCWWGG 1 cut(s) 392
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 4 cut(s) 206, 524, 767, 1321
FalI AAGNNNNNCTT 2 cut(s) 1122, 1154
FatI CATG 4 cut(s) 202, 520, 763, 1317
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 2 cut(s) 379, 813
FokI GGATG 1 cut(s) 1037
FriOI GRGCYC 2 cut(s) 93, 192
Fsp4HI GCNGC 2 cut(s) 379, 813
FspBI CTAG 2 cut(s) 1152, 1401
GluI GCNGC 2 cut(s) 379, 813
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 3 cut(s) 433, 531, 942
HaeIII GGCC 2 cut(s) 847, 901
HapII CCGG 2 cut(s) 837, 957
Hin1II CATG 4 cut(s) 206, 524, 767, 1321
HincII GTYRAC 1 cut(s) 1222
HindII GTYRAC 1 cut(s) 1222
HinfI GANTC 3 cut(s) 497, 625, 715
HpaII CCGG 2 cut(s) 837, 957
HphI GGTGA 3 cut(s) 599, 877, 1261
Hpy166II GTNNAC 4 cut(s) 64, 517, 1222, 1409
Hpy188I TCNGA 7 cut(s) 83, 88, 187, 276, 423, 1183, 1353
Hpy188III TCNNGA 8 cut(s) 296, 373, 412, 600, 639, 712, 764, 1289
Hpy8I GTNNAC 4 cut(s) 64, 517, 1222, 1409
HpyAV CCTTC 4 cut(s) 117, 418, 697, 990
HpyCH4III ACNGT 4 cut(s) 469, 910, 1005, 1345
HpyCH4V TGCA 4 cut(s) 381, 565, 1088, 1096
HpyF10VI GCNNNNNNNGC 2 cut(s) 734, 1144
HpyF3I CTNAG 8 cut(s) 80, 186, 273, 371, 387, 552, 696, 824
Hsp92II CATG 4 cut(s) 206, 524, 767, 1321
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 4 cut(s) 83, 574, 973, 1348
LguI GCTCTTC 1 cut(s) 1025
LmnI GCTCC 2 cut(s) 375, 456
Lsp1109I GCAGC 2 cut(s) 365, 799
LweI GCATC 2 cut(s) 605, 1075
MaeI CTAG 2 cut(s) 1152, 1401
MaeIII GTNAC 3 cut(s) 145, 1054, 1210
MalI GATC 4 cut(s) 85, 576, 975, 1350
MboI GATC 4 cut(s) 83, 574, 973, 1348
MboII GAAGA 7 cut(s) 16, 19, 37, 333, 929, 1042, 1248
MhlI GDGCHC 3 cut(s) 93, 192, 1121
MluCI AATT 9 cut(s) 262, 326, 682, 707, 1090, 1284, 1311, 1360, 1392
MmeI TCCRAC 5 cut(s) 61, 223, 723, 1086, 1161
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 4 cut(s) 1050, 1196, 1305, 1391
MslI CAYNNNNRTG 3 cut(s) 570, 768, 1208
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 2 cut(s) 837, 957
MspR9I CCNGG 1 cut(s) 837
MwoI GCNNNNNNNGC 2 cut(s) 734, 1144
NciI CCSGG 1 cut(s) 837
NdeII GATC 4 cut(s) 83, 574, 973, 1348
NheI GCTAGC 1 cut(s) 1400
NlaIII CATG 4 cut(s) 206, 524, 767, 1321
NmuCI GTSAC 1 cut(s) 1210
OliI CACNNNNGTG 1 cut(s) 1208
PagI TCATGA 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 497, 625, 715
PkrI GCNGC 2 cut(s) 380, 814
PshAI GACNNNNGTC 1 cut(s) 1217
PshBI ATTAAT 1 cut(s) 1305
Psp124BI GAGCTC 2 cut(s) 93, 192
PspFI CCCAGC 1 cut(s) 128
PspPI GGNCC 3 cut(s) 396, 845, 1227
PstNI CAGNNNCTG 2 cut(s) 378, 473
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 4 cut(s) 165, 440, 806, 1079
RsaNI GTAC 4 cut(s) 164, 439, 805, 1078
RseI CAYNNNNRTG 3 cut(s) 570, 768, 1208
SacI GAGCTC 2 cut(s) 93, 192
SapI GCTCTTC 1 cut(s) 1025
SaqAI TTAA 4 cut(s) 1050, 1196, 1305, 1391
SatI GCNGC 2 cut(s) 379, 813
Sau3AI GATC 4 cut(s) 83, 574, 973, 1348
Sau96I GGNCC 3 cut(s) 396, 845, 1227
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 3 cut(s) 93, 192, 1121
SfaNI GCATC 2 cut(s) 605, 1075
SinI GGWCC 2 cut(s) 396, 1227
SmiMI CAYNNNNRTG 3 cut(s) 570, 768, 1208
SmlI CTYRAG 2 cut(s) 1128, 1412
SmoI CTYRAG 2 cut(s) 1128, 1412
Sse9I AATT 9 cut(s) 262, 326, 682, 707, 1090, 1284, 1311, 1360, 1392
SsiI CCGC 2 cut(s) 334, 617
SspMI CTAG 2 cut(s) 1152, 1401
SstI GAGCTC 2 cut(s) 93, 192
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 4 cut(s) 469, 910, 1005, 1345
TaqI TCGA 5 cut(s) 638, 711, 721, 993, 1083
TasI AATT 9 cut(s) 262, 326, 682, 707, 1090, 1284, 1311, 1360, 1392
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 3 cut(s) 497, 625, 715
Tru1I TTAA 4 cut(s) 1050, 1196, 1305, 1391
Tru9I TTAA 4 cut(s) 1050, 1196, 1305, 1391
TscAI CASTG 4 cut(s) 514, 925, 1179, 1350
TseFI GTSAC 1 cut(s) 1210
TseI GCWGC 2 cut(s) 378, 812
Tsp45I GTSAC 1 cut(s) 1210
TspDTI ATGAA 4 cut(s) 527, 747, 752, 957
TspGWI ACGGA 1 cut(s) 1219
TspRI CASTG 4 cut(s) 514, 925, 1179, 1350
VpaK11BI GGWCC 2 cut(s) 396, 1227
VspI ATTAAT 1 cut(s) 1305
XapI RAATTY 4 cut(s) 682, 707, 1090, 1360
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 2 cut(s) 1152, 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.