Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002853

Physical Location & Seq

Genomic Coordinates Reverse (-)

32769112 .. 32771449

Gene Structure 2338 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1243300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1845 bp

ATGGGCTCTCCGTTGGCCCTCCTCCTCCTCCTCCTCCTCCCGCTCCTCCTTCACCAACCACTCTTTTCTGCCGCCAATGTCCACAAACTCAACCTCCTCCGATCCGAGCTCCTCTCCCAGCCCACCCTTCGAAACGACGCCGCACCAACTCTCTTCTTCGAAGTAACCAAACCCATAGAACTCCCCAAAACCAAGCCCTGCACCCAGCACGTTCTCCAGCACGACTTCGGGTACACATACGGCAAACCTCCGGTCTTTGCTAACTACAACCCTCCCTCGCACTGCCCATCTCAATCATTTTCCAAAATTGTCCTCGAGTGGAAAGCCACCTGCAGAGGGAGGCAATATGATCGAATTTTCGGGGTTTGGCTCGGCGGCGTTGAGCTTCTCAGGGGCTGCACGGCTGAGCCGACGCAGACTGGGATTGTTTGGAGTGTCAAGAAGGACATCACAAGGTACTATTCTTTGCTTCAGAAGAATCAAACACTAGCTGTTTATCTTGGGAATTTAGTAAATGAAACATATACTGGGATTTACCATGTGAATATTAGCATCCATTTTTACCCTGCTGAGAAGAATTTGAATTATAACTATGAGCAAAATTTGGTAAATTTGGATTCTGGGTACCATTCTTGGGCCGATTTAATCTTGCCCATTTCGAGAAATCAGCCTTTGAATGATGGGTTGTGGTTTGAAATCCAGAATTCGACCGATACACAGTTGAAGGAATTTGAGATTCCACAGAATGCTTATAGGGCTGTGTTGGAGGTGTATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCAAATCCTCCGAATGAGTACATTGCTGCAAACAATCTTAGTGGTACGCCTGGAAATGGGCCTTTTAGGGAGGTTGTGGTGAGTTTAGATGGTGAGGTTGTTGGTGCAGTCTGGCCTTTTACTGTGATTTTCACTGGAGGGGTCAATCCGCTCCTGTGGAGACCTATTACTGCAATTGGCTCGTTCGATCTCCCTTCTTATGATATTGAAATCACGCCCTTCTTAGGGAAGATATTGGATGGGAAGAGACACAAGTTTGGTTTTAGTGTTACAAATGCCTTGAATGTTTGGCTTCTTGATGCGAATTTGCATCTTTGGTTGGACAAGCGGAGCACGAAAACTGAAGGTGAGCTTTTGAAGCATAGTAGCTTGCCCCTTGTTGTTTCATTGGTTTCAGATTTCAAGGGTTTAAATGGCACATTTTTTACTAGGGCCGGCAGGTCTGTGTCATCGACTGGATGGGTGAAGTCCTCCTATGGGAATATCACAACTGATGCAATTCAAGACTTCCAGTACAGCAATACAATGGTCATGAGGAATGATGGTAATACACAGATAGTGAATCAGGTCATCCATTTCAACGATACAATCAATATCAGGCCGCCATCCTCCAGTATTCACTCAATGTCATCAAACAAAACATTTCCTCTTTACTTGTACACTGACTACATGGAGCAAGCAAATGAATCATATACAGTCGTTACAAATGTCGAATTGGGATTTATTGAGAAGAAGTCTGCAAGTGCTGGTTCACGATTCTCGAACAGCTCTCTCCAAAATCTGCAAAATGCCGAGGTTATTATCGTTGTAAAAAACAATCTGCTAGTTAGGGGATTGGGGAGTACACAGCAAGTGTATAGATTCGATGGTCCCCAATCATGTTATTTCAGAAACATAAGCAGCTCAAACTACACCATACTCTATGATAAGGTGAGGCTTACATGCACCGAAAAAAGGGAGTCTAATTTGGATTTTGGCTTAAGCAGGCTGTGGCCTTTTGGTGCTCAAAGGAACTTTCCTGGTCCCGGATTTACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PNGaseA

Protein Analysis

615

Amino Acids

69.26

Weight (kDa)

6.93

Isoelectric Point (pI)

41.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PNGaseA	PF12222	64 - 389	2.8e-106	Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C	PF25156	409 - 486	1e-21	PNGase_A C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14920
fragaria_vesca	FvH4_3g18311 FvH4_3g18320 FvH4_3g18320
malus_domestica	MD03G1242900.v1.1 MD03G1243300.v1.1 MD03G1243400.v1.1
prunus_persica	Prupe.4G164900_v2.0.a1
pyrus_communis	pycom11g23210
rosa_chinensis	RchiOBHm_Chr5g0030951 RchiOBHm_Chr5g0030961 RchiOBHm_Chr5g0030971
rosa_laevigata	RLG00000033274 RLG00000033275 RLG00000033276
rosa_multiflora	Rmu_sc0001974.1_g000004 Rmu_sc0001974.1_g000008 Rmu_sc0001974.1_g000009 Rmu_sc0040817.1_g000002
rosa_roxburghii	Rroxscaffold_1G00049310 Rroxscaffold_1G00049320
rosa_rugosa	Rorug05G0121600 Rorug05G0121700 Rorug05G0121900
rosa_samantha	Rh5AG214900 Rh5AG215000 Rh5AG215200 Rh5BG213900 Rh5BG214000 Rh5BG214200 Rh5CG237800 Rh5CG237900 Rh5CG238100 Rh5DG218500 Rh5DG218600 Rh5DG218800
rosa_wichuraiana	Rw5G019660 Rw5G019670 Rw5G019680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	588
AarI	CACCTGC	1 cut(s)	338
Acc36I	ACCTGC	2 cut(s)	338, 1236
Acc65I	GGTACC	1 cut(s)	624
AccB1I	GGYRCC	1 cut(s)	624
AccBSI	CCGCTC	2 cut(s)	43, 958
AciI	CCGC	7 cut(s)	41, 72, 141, 375, 956, 1135, 1409
AclWI	GGATC	1 cut(s)	96
AcsI	RAATTY	8 cut(s)	354, 505, 577, 601, 610, 703, 728, 1111
AcuI	CTGAAG	2 cut(s)	455, 1170
AcyI	GRCGYC	1 cut(s)	138
AfaI	GTAC	8 cut(s)	233, 458, 626, 827, 853, 1322, 1466, 1651
AfiI	CCNNNNNNNGG	8 cut(s)	336, 634, 863, 1031, 1032, 1284, 1761, 1832
AflII	CTTAAG	1 cut(s)	1786
AjnI	CCWGG	2 cut(s)	856, 1825
AluBI	AGCT	7 cut(s)	109, 385, 491, 1159, 1176, 1575, 1710
AluI	AGCT	7 cut(s)	109, 385, 491, 1159, 1176, 1575, 1710
Alw21I	GWGCWC	3 cut(s)	111, 1142, 1813
Alw26I	GTCTC	2 cut(s)	961, 1048
AlwI	GGATC	1 cut(s)	96
AlwNI	CAGNNNCTG	1 cut(s)	396
Ama87I	CYCGRG	1 cut(s)	314
AoxI	GGCC	7 cut(s)	15, 636, 866, 920, 1239, 1406, 1799
ApeKI	GCWGC	3 cut(s)	396, 833, 1707
ApoI	RAATTY	8 cut(s)	354, 505, 577, 601, 610, 703, 728, 1111
ArsI	GACNNNNNNTTYG	2 cut(s)	237, 269
Asp718I	GGTACC	1 cut(s)	624
AspS9I	GGNCC	6 cut(s)	16, 636, 866, 1239, 1676, 1829
AsuC2I	CCSGG	1 cut(s)	1833
AsuHPI	GGTGA	6 cut(s)	44, 898, 911, 1166, 1282, 1750
AsuII	TTCGAA	2 cut(s)	130, 159
AvaI	CYCGRG	1 cut(s)	314
AvaII	GGWCC	2 cut(s)	1676, 1829
BanI	GGYRCC	1 cut(s)	624
BanII	GRGCYC	2 cut(s)	8, 111
Bbv12I	GWGCWC	3 cut(s)	111, 1142, 1813
BbvI	GCAGC	3 cut(s)	383, 820, 1719
BccI	CCATC	8 cut(s)	295, 674, 890, 1040, 1260, 1343, 1420, 1667
BceAI	ACGGC	2 cut(s)	256, 417
BcgI	CGANNNNNNTGC	4 cut(s)	332, 366, 969, 1003
BciT130I	CCWGG	2 cut(s)	858, 1827
BcnI	CCSGG	1 cut(s)	1833
BcoDI	GTCTC	2 cut(s)	961, 1048
BfaI	CTAG	3 cut(s)	488, 1236, 1631
BfmI	CTRYAG	1 cut(s)	331
BfrI	CTTAAG	1 cut(s)	1786
BfuAI	ACCTGC	2 cut(s)	338, 1236
BisI	GCNGC	7 cut(s)	72, 141, 376, 397, 834, 1409, 1708
BlpI	GCTNAGC	1 cut(s)	405
BlsI	GCNGC	7 cut(s)	73, 142, 377, 398, 835, 1410, 1709
Bme1390I	CCNGG	3 cut(s)	858, 1827, 1833
Bme18I	GGWCC	2 cut(s)	1676, 1829
BmeT110I	CYCGRG	1 cut(s)	314
BmgT120I	GGNCC	6 cut(s)	16, 636, 866, 1239, 1676, 1829
BmiI	GGNNCC	3 cut(s)	626, 1678, 1831
BmrFI	CCNGG	3 cut(s)	858, 1827, 1833
BmrI	ACTGGG	2 cut(s)	429, 537
BmsI	GCATC	4 cut(s)	561, 1096, 1126, 1291
BmuI	ACTGGG	2 cut(s)	429, 537
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
BpmI	CTGGAG	3 cut(s)	200, 963, 1402
Bpu1102I	GCTNAGC	1 cut(s)	405
Bpu14I	TTCGAA	2 cut(s)	130, 159
BpuMI	CCSGG	1 cut(s)	1833
BsaHI	GRCGYC	1 cut(s)	138
BsaI	GGTCTC	1 cut(s)	961
BsaJI	CCNNGG	1 cut(s)	1599
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	78, 108
Bsc4I	CCNNNNNNNGG	8 cut(s)	336, 634, 863, 1031, 1032, 1284, 1761, 1832
Bse118I	RCCGGY	1 cut(s)	1241
Bse1I	ACTGG	6 cut(s)	424, 532, 946, 1267, 1318, 1419
Bse3DI	GCAATG	1 cut(s)	828
BseBI	CCWGG	2 cut(s)	858, 1827
BseDI	CCNNGG	1 cut(s)	1599
BseGI	GGATG	5 cut(s)	552, 1051, 1271, 1377, 1412
BseLI	CCNNNNNNNGG	8 cut(s)	336, 634, 863, 1031, 1032, 1284, 1761, 1832
BseMI	GCAATG	1 cut(s)	828
BseMII	CTCAG	3 cut(s)	396, 403, 561
BseNI	ACTGG	6 cut(s)	424, 532, 946, 1267, 1318, 1419
BseRI	GAGGAG	9 cut(s)	11, 14, 17, 20, 23, 26, 35, 86, 101
BseXI	GCAGC	3 cut(s)	383, 820, 1719
BseYI	CCCAGC	2 cut(s)	117, 204
BsgI	GTGCAG	3 cut(s)	184, 382, 933
Bsh1285I	CGRYCG	1 cut(s)	711
BshFI	GGCC	7 cut(s)	17, 638, 868, 922, 1241, 1408, 1801
BshNI	GGYRCC	1 cut(s)	624
BsiEI	CGRYCG	1 cut(s)	711
BsiHKAI	GWGCWC	3 cut(s)	111, 1142, 1813
BsiHKCI	CYCGRG	1 cut(s)	314
BsiSI	CCGG	3 cut(s)	251, 1242, 1833
BslFI	GGGAC	2 cut(s)	1662, 1815
BslI	CCNNNNNNNGG	8 cut(s)	336, 634, 863, 1031, 1032, 1284, 1761, 1832
BsmAI	GTCTC	2 cut(s)	961, 1048
BsmFI	GGGAC	2 cut(s)	1662, 1815
BsmI	GAATGC	1 cut(s)	751
BsnI	GGCC	7 cut(s)	17, 638, 868, 922, 1241, 1408, 1801
Bso31I	GGTCTC	1 cut(s)	961
BsoBI	CYCGRG	1 cut(s)	314
Bsp119I	TTCGAA	2 cut(s)	130, 159
Bsp1286I	GDGCHC	4 cut(s)	8, 111, 1142, 1813
Bsp1407I	TGTACA	1 cut(s)	1464
Bsp143I	GATC	3 cut(s)	101, 349, 994
Bsp1720I	GCTNAGC	1 cut(s)	405
BspACI	CCGC	7 cut(s)	41, 72, 141, 375, 956, 1135, 1409
BspANI	GGCC	7 cut(s)	17, 638, 868, 922, 1241, 1408, 1801
BspCNI	CTCAG	3 cut(s)	397, 402, 562
BspHI	TCATGA	2 cut(s)	784, 1338
BspLI	GGNNCC	3 cut(s)	626, 1678, 1831
BspMAI	CTGCAG	1 cut(s)	335
BspMI	ACCTGC	2 cut(s)	338, 1236
BspPI	GGATC	1 cut(s)	96
BspT104I	TTCGAA	2 cut(s)	130, 159
BspT107I	GGYRCC	1 cut(s)	624
BspTI	CTTAAG	1 cut(s)	1786
BspTNI	GGTCTC	1 cut(s)	961
BsrBI	CCGCTC	2 cut(s)	43, 958
BsrDI	GCAATG	1 cut(s)	828
BsrFI	RCCGGY	1 cut(s)	1241
BsrGI	TGTACA	1 cut(s)	1464
BsrI	ACTGG	6 cut(s)	424, 532, 946, 1267, 1318, 1419
BssAI	RCCGGY	1 cut(s)	1241
BssECI	CCNNGG	1 cut(s)	1599
BssMI	GATC	3 cut(s)	101, 349, 994
BssNI	GRCGYC	1 cut(s)	138
Bst2UI	CCWGG	2 cut(s)	858, 1827
Bst4CI	ACNGT	3 cut(s)	720, 931, 1504
Bst6I	CTCTTC	2 cut(s)	158, 1046
BstACI	GRCGYC	1 cut(s)	138
BstAFI	CTTAAG	1 cut(s)	1786
BstAUI	TGTACA	1 cut(s)	1464
BstBI	TTCGAA	2 cut(s)	130, 159
BstC8I	GCNNGC	4 cut(s)	1178, 1243, 1485, 1793
BstDEI	CTNAG	5 cut(s)	389, 405, 570, 845, 1030
BstF5I	GGATG	5 cut(s)	552, 1051, 1271, 1377, 1412
BstKTI	GATC	3 cut(s)	104, 352, 997
BstMAI	GTCTC	2 cut(s)	961, 1048
BstMBI	GATC	3 cut(s)	101, 349, 994
BstMCI	CGRYCG	1 cut(s)	711
BstMWI	GCNNNNNNNGC	2 cut(s)	755, 1165
BstNI	CCWGG	2 cut(s)	858, 1827
BstNSI	RCATGY	1 cut(s)	1752
BstSCI	CCNGG	3 cut(s)	856, 1825, 1831
BstSFI	CTRYAG	1 cut(s)	331
BstV1I	GCAGC	3 cut(s)	383, 820, 1719
BsuRI	GGCC	7 cut(s)	17, 638, 868, 922, 1241, 1408, 1801
BtsCI	GGATG	5 cut(s)	552, 1051, 1271, 1377, 1412
BtsI	GCAGTG	1 cut(s)	280
BtsIMutI	CAGTG	3 cut(s)	280, 939, 1467
BveI	ACCTGC	2 cut(s)	338, 1236
Cac8I	GCNNGC	4 cut(s)	1178, 1243, 1485, 1793
CaiI	CAGNNNCTG	1 cut(s)	396
CciI	TCATGA	2 cut(s)	784, 1338
Cfr10I	RCCGGY	1 cut(s)	1241
Cfr13I	GGNCC	6 cut(s)	16, 636, 866, 1239, 1676, 1829
CseI	GACGC	2 cut(s)	146, 421
Csp6I	GTAC	8 cut(s)	232, 457, 625, 826, 852, 1321, 1465, 1650
CspCI	CAANNNNNGTGG	2 cut(s)	829, 864
CviAII	CATG	6 cut(s)	539, 785, 1339, 1477, 1686, 1749
CviQI	GTAC	8 cut(s)	232, 457, 625, 826, 852, 1321, 1465, 1650
DdeI	CTNAG	5 cut(s)	389, 405, 570, 845, 1030
DpnI	GATC	3 cut(s)	103, 351, 996
DpnII	GATC	3 cut(s)	101, 349, 994
DraI	TTTAAA	1 cut(s)	1218
Eam1104I	CTCTTC	2 cut(s)	158, 1046
EarI	CTCTTC	2 cut(s)	158, 1046
Ecl136II	GAGCTC	1 cut(s)	109
Eco24I	GRGCYC	2 cut(s)	8, 111
Eco31I	GGTCTC	1 cut(s)	961
Eco47I	GGWCC	2 cut(s)	1676, 1829
Eco53kI	GAGCTC	1 cut(s)	109
Eco57I	CTGAAG	2 cut(s)	455, 1170
Eco88I	CYCGRG	1 cut(s)	314
EcoICRI	GAGCTC	1 cut(s)	109
EcoRI	GAATTC	1 cut(s)	703
EcoRII	CCWGG	2 cut(s)	856, 1825
EcoT38I	GRGCYC	2 cut(s)	8, 111
FaeI	CATG	6 cut(s)	542, 788, 1342, 1480, 1689, 1752
FalI	AAGNNNNNCTT	2 cut(s)	1143, 1175
FaqI	GGGAC	2 cut(s)	1662, 1815
FatI	CATG	6 cut(s)	538, 784, 1338, 1476, 1685, 1748
FauI	CCCGC	1 cut(s)	48
Fnu4HI	GCNGC	7 cut(s)	72, 141, 376, 397, 834, 1409, 1708
FokI	GGATG	5 cut(s)	539, 1058, 1278, 1364, 1399
FriOI	GRGCYC	2 cut(s)	8, 111
Fsp4HI	GCNGC	7 cut(s)	72, 141, 376, 397, 834, 1409, 1708
FspBI	CTAG	3 cut(s)	488, 1236, 1631
GluI	GCNGC	7 cut(s)	72, 141, 376, 397, 834, 1409, 1708
GsaI	CCCAGC	2 cut(s)	121, 208
GsuI	CTGGAG	3 cut(s)	200, 963, 1402
HaeIII	GGCC	7 cut(s)	17, 638, 868, 922, 1241, 1408, 1801
HapII	CCGG	3 cut(s)	251, 1242, 1833
HgaI	GACGC	2 cut(s)	146, 421
Hin1I	GRCGYC	1 cut(s)	138
Hin1II	CATG	6 cut(s)	542, 788, 1342, 1480, 1689, 1752
HinfI	GANTC	8 cut(s)	478, 617, 736, 1369, 1493, 1563, 1668, 1766
HpaII	CCGG	3 cut(s)	251, 1242, 1833
HphI	GGTGA	6 cut(s)	44, 898, 911, 1166, 1282, 1750
Hpy166II	GTNNAC	5 cut(s)	82, 234, 1467, 1559, 1652
Hpy188I	TCNGA	6 cut(s)	101, 106, 474, 819, 1204, 1697
Hpy188III	TCNNGA	9 cut(s)	439, 660, 700, 785, 1103, 1310, 1339, 1560, 1567
Hpy8I	GTNNAC	5 cut(s)	82, 234, 1467, 1559, 1652
Hpy99I	CGWCG	2 cut(s)	140, 415
HpyAV	CCTTC	7 cut(s)	59, 137, 436, 718, 1011, 1036, 1145
HpyCH4III	ACNGT	3 cut(s)	720, 931, 1504
HpyCH4IV	ACGT	1 cut(s)	210
HpyF10VI	GCNNNNNNNGC	2 cut(s)	755, 1165
HpyF3I	CTNAG	5 cut(s)	389, 405, 570, 845, 1030
HpySE526I	ACGT	1 cut(s)	210
Hsp92I	GRCGYC	1 cut(s)	138
Hsp92II	CATG	6 cut(s)	542, 788, 1342, 1480, 1689, 1752
KpnI	GGTACC	1 cut(s)	628
KroI	GCCGGC	1 cut(s)	1241
KroNI	GCCGGC	1 cut(s)	1243
Kzo9I	GATC	3 cut(s)	101, 349, 994
LmnI	GCTCC	5 cut(s)	48, 114, 963, 1137, 1480
Lsp1109I	GCAGC	3 cut(s)	383, 820, 1719
LweI	GCATC	4 cut(s)	561, 1096, 1126, 1291
MaeI	CTAG	3 cut(s)	488, 1236, 1631
MaeII	ACGT	1 cut(s)	210
MaeIII	GTNAC	3 cut(s)	163, 1075, 1507
MalI	GATC	3 cut(s)	103, 351, 996
MbiI	CCGCTC	2 cut(s)	43, 958
MboI	GATC	3 cut(s)	101, 349, 994
MboII	GAAGA	7 cut(s)	145, 148, 487, 586, 1048, 1063, 1549
MfeI	CAATTG	1 cut(s)	981
MhlI	GDGCHC	4 cut(s)	8, 111, 1142, 1813
MlyI	GAGTC	1 cut(s)	1775
MmeI	TCCRAC	2 cut(s)	744, 1107
MroNI	GCCGGC	1 cut(s)	1241
MseI	TTAA	3 cut(s)	644, 1217, 1787
MslI	CAYNNNNRTG	1 cut(s)	789
MspCI	CTTAAG	1 cut(s)	1786
MspI	CCGG	3 cut(s)	251, 1242, 1833
MspR9I	CCNGG	3 cut(s)	858, 1827, 1833
MunI	CAATTG	1 cut(s)	981
Mva1269I	GAATGC	1 cut(s)	751
MvaI	CCWGG	2 cut(s)	858, 1827
MwoI	GCNNNNNNNGC	2 cut(s)	755, 1165
NaeI	GCCGGC	1 cut(s)	1243
NciI	CCSGG	1 cut(s)	1833
NdeII	GATC	3 cut(s)	101, 349, 994
NgoMIV	GCCGGC	1 cut(s)	1241
NlaIII	CATG	6 cut(s)	542, 788, 1342, 1480, 1689, 1752
NlaIV	GGNNCC	3 cut(s)	626, 1678, 1831
NmeAIII	GCCGAG	2 cut(s)	351, 1624
NspI	RCATGY	1 cut(s)	1752
NspV	TTCGAA	2 cut(s)	130, 159
PaeR7I	CTCGAG	1 cut(s)	314
PagI	TCATGA	2 cut(s)	784, 1338
PaqCI	CACCTGC	1 cut(s)	338
PcsI	WCGNNNNNNNCGW	1 cut(s)	407
PctI	GAATGC	1 cut(s)	751
PdiI	GCCGGC	1 cut(s)	1243
PfeI	GAWTC	7 cut(s)	478, 617, 736, 1369, 1493, 1563, 1668
PfoI	TCCNGGA	1 cut(s)	1831
PkrI	GCNGC	7 cut(s)	73, 142, 377, 398, 835, 1410, 1709
PleI	GAGTC	1 cut(s)	1774
PpsI	GAGTC	1 cut(s)	1774
PsiI	TTATAA	1 cut(s)	588
Psp124BI	GAGCTC	1 cut(s)	111
Psp6I	CCWGG	2 cut(s)	856, 1825
PspFI	CCCAGC	2 cut(s)	117, 204
PspGI	CCWGG	2 cut(s)	856, 1825
PspN4I	GGNNCC	3 cut(s)	626, 1678, 1831
PspPI	GGNCC	6 cut(s)	16, 636, 866, 1239, 1676, 1829
PspXI	VCTCGAGB	1 cut(s)	314
PstI	CTGCAG	1 cut(s)	335
PstNI	CAGNNNCTG	1 cut(s)	396
RsaI	GTAC	8 cut(s)	233, 458, 626, 827, 853, 1322, 1466, 1651
RsaNI	GTAC	8 cut(s)	232, 457, 625, 826, 852, 1321, 1465, 1650
RseI	CAYNNNNRTG	1 cut(s)	789
SacI	GAGCTC	1 cut(s)	111
SaqAI	TTAA	3 cut(s)	644, 1217, 1787
SatI	GCNGC	7 cut(s)	72, 141, 376, 397, 834, 1409, 1708
Sau3AI	GATC	3 cut(s)	101, 349, 994
Sau96I	GGNCC	6 cut(s)	16, 636, 866, 1239, 1676, 1829
SchI	GAGTC	1 cut(s)	1775
ScrFI	CCNGG	3 cut(s)	858, 1827, 1833
SduI	GDGCHC	4 cut(s)	8, 111, 1142, 1813
SfaNI	GCATC	4 cut(s)	561, 1096, 1126, 1291
SfcI	CTRYAG	1 cut(s)	331
Sfr274I	CTCGAG	1 cut(s)	314
SfuI	TTCGAA	2 cut(s)	130, 159
SinI	GGWCC	2 cut(s)	1676, 1829
SlaI	CTCGAG	1 cut(s)	314
SmiMI	CAYNNNNRTG	1 cut(s)	789
SmlI	CTYRAG	2 cut(s)	314, 1786
SmoI	CTYRAG	2 cut(s)	314, 1786
SsiI	CCGC	7 cut(s)	41, 72, 141, 375, 956, 1135, 1409
SspI	AATATT	1 cut(s)	547
SspMI	CTAG	3 cut(s)	488, 1236, 1631
SstI	GAGCTC	1 cut(s)	111
StyD4I	CCNGG	3 cut(s)	856, 1825, 1831
TaaI	ACNGT	3 cut(s)	720, 931, 1504
TaiI	ACGT	1 cut(s)	213
TaqII	GACCGA	1 cut(s)	725
TatI	WGTACW	4 cut(s)	825, 1320, 1464, 1649
TauI	GCSGC	4 cut(s)	74, 143, 378, 1411
TfiI	GAWTC	7 cut(s)	478, 617, 736, 1369, 1493, 1563, 1668
Tru1I	TTAA	3 cut(s)	644, 1217, 1787
Tru9I	TTAA	3 cut(s)	644, 1217, 1787
TscAI	CASTG	3 cut(s)	287, 946, 1474
TseI	GCWGC	3 cut(s)	396, 833, 1707
TspDTI	ATGAA	5 cut(s)	531, 768, 773, 1182, 1506
TspRI	CASTG	3 cut(s)	287, 946, 1474
Vha464I	CTTAAG	1 cut(s)	1786
VpaK11BI	GGWCC	2 cut(s)	1676, 1829
XapI	RAATTY	8 cut(s)	354, 505, 577, 601, 610, 703, 728, 1111
XceI	RCATGY	1 cut(s)	1752
XhoI	CTCGAG	1 cut(s)	314
XspI	CTAG	3 cut(s)	488, 1236, 1631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.