Rh5AG215200

peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002853
Physical Location & Seq
Forward (+)
25317635 .. 25320443
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG215200.1

Sequence Viewer

Length: 1122 bp
ATGGCTACTCTATTGCCTCTCCTCTTCTTCCTCCTCCAACCCCTCTTCTCCATTGCCAATGTCCACAAACTCAACCAACTCAGATCAGAGCTCATCTCCCAATCCCCTTCTCTCAATGACACCCCACCCAGCCTTGACTTTGAAGTAACCAAACCCATTCCAGTACCCAAAACCAAGCCTTGTTCTGAGCTCATTCTCCAACATGATTTTGCCTACACTTACGGAAAACCACCAGTCTTTGCTAACTACACTCCTCCCTCAAATTGCCCGTCTCAGAAGTTCTCCAAGATTGTTCTTGAATGGAAAGCTACAAGCAAAGGAAGACAATTTGACCGCATTTTTGGGGTTTGGCTTTCTGGTGTTGAGCTTCTCAGGAGCTGCACAGCTGAGCCAAGGACCAACGGGATTGTCTGGAGTGTTCAGAAGGACATCAGCAGGTACTATTCGTTGCTCCAAAAGAAACAGACAGTTGCTGTTTATCTTGGCAGTTTGATTGATTCAACATACACTGGAGTTTACCATGTGAATATAAGTTTTCATTTTTATCCTGCTGAGGAGAGTTTGCATATTTATGATCAAAATGTGGGTGATTTGGCATCTGGATATCATTCTTGGGCGGATTTGATTCTGCCCATTTCGAGAAATCTGCCTTTGACTGATGGGTTGTGGTTTGAAGTTCAGAATTCAACTGATACAGAGTTGAAGGAATTCGAGATTCCTCGAAATGCTTATAGGGCTGTGTTGGAGGTTTATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCCAATCCTACTAATGAGTACATTGCTGCCAACAACCTTAGTGGCACGCCCGGAAATGGGCCTTTCAGGGAGGTTGTGGTGAGTTTAGATGGTGTGGTTGTTGGTGCTGTTTGGCCTTTTACTGTGATTTACACTGGAGGGTTCAATCCTCTTCTGTGGAGACCCATTACCGGCATTGGTTCATTTGATCTACCTTCTTATGATATCGAAATCACACCGTTTTTAGGGAACATATTGGATGGGAAGAGCCACAAGATTGGGTTTAATGTTACCAATGCCTTGAATGATAGGCGAGTTCTTTTAATTGAAGCTAGCTGTCCACTTGAGCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

373

Amino Acids

41.98

Weight (kDa)

5.41

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 57 - 357 4.3e-101 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 426
AciI CCGC 2 cut(s) 334, 617
AcsI RAATTY 2 cut(s) 682, 707
AfaI GTAC 3 cut(s) 165, 440, 806
AfiI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
AgsI TTSAA 9 cut(s) 143, 299, 501, 674, 687, 703, 931, 1069, 1094
AluBI AGCT 9 cut(s) 91, 190, 308, 367, 378, 386, 1097, 1101, 1114
AluI AGCT 9 cut(s) 91, 190, 308, 367, 378, 386, 1097, 1101, 1114
Alw21I GWGCWC 2 cut(s) 93, 192
Alw26I GTCTC 2 cut(s) 276, 940
AlwNI CAGNNNCTG 2 cut(s) 378, 473
AoxI GGCC 2 cut(s) 845, 899
ApeKI GCWGC 2 cut(s) 378, 812
ApoI RAATTY 2 cut(s) 682, 707
ArsI GACNNNNNNTTYG 4 cut(s) 254, 286, 323, 355
Asp700I GAANNNNTTC 1 cut(s) 707
AspS9I GGNCC 2 cut(s) 396, 845
AsuC2I CCSGG 1 cut(s) 837
AsuHPI GGTGA 2 cut(s) 599, 877
AsuNHI GCTAGC 1 cut(s) 1097
AvaII GGWCC 1 cut(s) 396
BanII GRGCYC 2 cut(s) 93, 192
BbsI GAAGAC 1 cut(s) 328
Bbv12I GWGCWC 2 cut(s) 93, 192
BbvCI CCTCAGC 1 cut(s) 552
BbvI GCAGC 2 cut(s) 365, 799
BccI CCATC 3 cut(s) 653, 869, 1019
BcgI CGANNNNNNTGC 2 cut(s) 628, 662
BclI TGATCA 1 cut(s) 574
BcnI CCSGG 1 cut(s) 837
BcoDI GTCTC 2 cut(s) 276, 940
BfaI CTAG 1 cut(s) 1098
BfuAI ACCTGC 1 cut(s) 426
BisI GCNGC 2 cut(s) 379, 813
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 2 cut(s) 380, 814
Bme1390I CCNGG 1 cut(s) 837
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 2 cut(s) 396, 845
BmrFI CCNGG 1 cut(s) 837
BmsI GCATC 1 cut(s) 605
BmtI GCTAGC 1 cut(s) 1101
BpiI GAAGAC 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpmI CTGGAG 3 cut(s) 433, 531, 942
Bpu10I CCTNAGC 1 cut(s) 552
Bpu1102I GCTNAGC 1 cut(s) 387
BpuMI CCSGG 1 cut(s) 837
BsaI GGTCTC 1 cut(s) 940
BsaJI CCNNGG 1 cut(s) 392
Bsc4I CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 4 cut(s) 161, 233, 514, 925
Bse3DI GCAATG 2 cut(s) 51, 807
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 1 cut(s) 1030
BseLI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
BseMI GCAATG 2 cut(s) 51, 807
BseMII CTCAG 6 cut(s) 94, 177, 287, 378, 385, 543
BseNI ACTGG 4 cut(s) 161, 233, 514, 925
BseRI GAGGAG 4 cut(s) 11, 23, 243, 569
BseXI GCAGC 2 cut(s) 365, 799
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 364
BshFI GGCC 2 cut(s) 847, 901
BsiHKAI GWGCWC 2 cut(s) 93, 192
BsiSI CCGG 2 cut(s) 837, 957
BslI CCNNNNNNNGG 4 cut(s) 842, 843, 956, 1010
BsmAI GTCTC 2 cut(s) 276, 940
BsmBI CGTCTC 1 cut(s) 276
BsnI GGCC 2 cut(s) 847, 901
Bso31I GGTCTC 1 cut(s) 940
Bsp1286I GDGCHC 2 cut(s) 93, 192
Bsp143I GATC 3 cut(s) 83, 574, 973
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 2 cut(s) 334, 617
BspANI GGCC 2 cut(s) 847, 901
BspCNI CTCAG 6 cut(s) 93, 178, 286, 379, 384, 544
BspHI TCATGA 1 cut(s) 763
BspMI ACCTGC 1 cut(s) 426
BspOI GCTAGC 1 cut(s) 1101
BspQI GCTCTTC 1 cut(s) 1025
BspTNI GGTCTC 1 cut(s) 940
BsrDI GCAATG 2 cut(s) 51, 807
BsrFI RCCGGY 1 cut(s) 956
BsrI ACTGG 4 cut(s) 161, 233, 514, 925
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 3 cut(s) 83, 574, 973
BssT1I CCWWGG 1 cut(s) 392
Bst4CI ACNGT 3 cut(s) 469, 910, 1005
Bst6I CTCTTC 4 cut(s) 29, 50, 942, 1025
BstC8I GCNNGC 2 cut(s) 833, 1099
BstDEI CTNAG 7 cut(s) 80, 186, 273, 371, 387, 552, 824
BstF5I GGATG 1 cut(s) 1030
BstKTI GATC 3 cut(s) 86, 577, 976
BstMAI GTCTC 2 cut(s) 276, 940
BstMBI GATC 3 cut(s) 83, 574, 973
BstMWI GCNNNNNNNGC 1 cut(s) 734
BstSCI CCNGG 1 cut(s) 835
BstV1I GCAGC 2 cut(s) 365, 799
BstV2I GAAGAC 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 1043
BsuRI GGCC 2 cut(s) 847, 901
BtsCI GGATG 1 cut(s) 1030
BtsIMutI CAGTG 2 cut(s) 507, 918
BveI ACCTGC 1 cut(s) 426
Cac8I GCNNGC 2 cut(s) 833, 1099
CaiI CAGNNNCTG 2 cut(s) 378, 473
CciI TCATGA 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 2 cut(s) 396, 845
Csp6I GTAC 3 cut(s) 164, 439, 805
CspCI CAANNNNNGTGG 2 cut(s) 808, 843
CviAII CATG 3 cut(s) 203, 521, 764
CviQI GTAC 3 cut(s) 164, 439, 805
DdeI CTNAG 7 cut(s) 80, 186, 273, 371, 387, 552, 824
DpnI GATC 3 cut(s) 85, 576, 975
DpnII GATC 3 cut(s) 83, 574, 973
Eam1104I CTCTTC 4 cut(s) 29, 50, 942, 1025
EarI CTCTTC 4 cut(s) 29, 50, 942, 1025
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 2 cut(s) 91, 190
Eco130I CCWWGG 1 cut(s) 392
Eco24I GRGCYC 2 cut(s) 93, 192
Eco31I GGTCTC 1 cut(s) 940
Eco32I GATATC 2 cut(s) 605, 991
Eco47I GGWCC 1 cut(s) 396
Eco53kI GAGCTC 2 cut(s) 91, 190
EcoICRI GAGCTC 2 cut(s) 91, 190
EcoRI GAATTC 2 cut(s) 682, 707
EcoRV GATATC 2 cut(s) 605, 991
EcoT14I CCWWGG 1 cut(s) 392
EcoT38I GRGCYC 2 cut(s) 93, 192
ErhI CCWWGG 1 cut(s) 392
Esp3I CGTCTC 1 cut(s) 276
FaeI CATG 3 cut(s) 206, 524, 767
FatI CATG 3 cut(s) 202, 520, 763
FbaI TGATCA 1 cut(s) 574
Fnu4HI GCNGC 2 cut(s) 379, 813
FokI GGATG 1 cut(s) 1037
FriOI GRGCYC 2 cut(s) 93, 192
Fsp4HI GCNGC 2 cut(s) 379, 813
FspBI CTAG 1 cut(s) 1098
GluI GCNGC 2 cut(s) 379, 813
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 3 cut(s) 433, 531, 942
HaeIII GGCC 2 cut(s) 847, 901
HapII CCGG 2 cut(s) 837, 957
Hin1II CATG 3 cut(s) 206, 524, 767
HinfI GANTC 3 cut(s) 497, 625, 715
HpaII CCGG 2 cut(s) 837, 957
HphI GGTGA 2 cut(s) 599, 877
Hpy166II GTNNAC 3 cut(s) 64, 517, 1106
Hpy188I TCNGA 6 cut(s) 83, 88, 187, 276, 423, 681
Hpy188III TCNNGA 7 cut(s) 296, 373, 412, 600, 639, 712, 764
Hpy8I GTNNAC 3 cut(s) 64, 517, 1106
HpyAV CCTTC 4 cut(s) 117, 418, 697, 990
HpyCH4III ACNGT 3 cut(s) 469, 910, 1005
HpyCH4V TGCA 2 cut(s) 381, 565
HpyF10VI GCNNNNNNNGC 1 cut(s) 734
HpyF3I CTNAG 7 cut(s) 80, 186, 273, 371, 387, 552, 824
Hsp92II CATG 3 cut(s) 206, 524, 767
Ksp22I TGATCA 1 cut(s) 574
Kzo9I GATC 3 cut(s) 83, 574, 973
LguI GCTCTTC 1 cut(s) 1025
LmnI GCTCC 2 cut(s) 375, 456
Lsp1109I GCAGC 2 cut(s) 365, 799
LweI GCATC 1 cut(s) 605
MaeI CTAG 1 cut(s) 1098
MaeIII GTNAC 2 cut(s) 145, 1054
MalI GATC 3 cut(s) 85, 576, 975
MboI GATC 3 cut(s) 83, 574, 973
MboII GAAGA 6 cut(s) 16, 19, 37, 333, 929, 1042
MhlI GDGCHC 2 cut(s) 93, 192
MluCI AATT 5 cut(s) 262, 326, 682, 707, 1089
MmeI TCCRAC 3 cut(s) 61, 223, 723
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 2 cut(s) 1050, 1088
MslI CAYNNNNRTG 2 cut(s) 570, 768
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 2 cut(s) 837, 957
MspR9I CCNGG 1 cut(s) 837
MwoI GCNNNNNNNGC 1 cut(s) 734
NciI CCSGG 1 cut(s) 837
NdeII GATC 3 cut(s) 83, 574, 973
NheI GCTAGC 1 cut(s) 1097
NlaIII CATG 3 cut(s) 206, 524, 767
PagI TCATGA 1 cut(s) 763
PciSI GCTCTTC 1 cut(s) 1025
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 3 cut(s) 497, 625, 715
PkrI GCNGC 2 cut(s) 380, 814
Psp124BI GAGCTC 2 cut(s) 93, 192
PspFI CCCAGC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 396, 845
PstNI CAGNNNCTG 2 cut(s) 378, 473
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 3 cut(s) 165, 440, 806
RsaNI GTAC 3 cut(s) 164, 439, 805
RseI CAYNNNNRTG 2 cut(s) 570, 768
SacI GAGCTC 2 cut(s) 93, 192
SapI GCTCTTC 1 cut(s) 1025
SaqAI TTAA 2 cut(s) 1050, 1088
SatI GCNGC 2 cut(s) 379, 813
Sau3AI GATC 3 cut(s) 83, 574, 973
Sau96I GGNCC 2 cut(s) 396, 845
ScrFI CCNGG 1 cut(s) 837
SduI GDGCHC 2 cut(s) 93, 192
SfaNI GCATC 1 cut(s) 605
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 2 cut(s) 570, 768
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
Sse9I AATT 5 cut(s) 262, 326, 682, 707, 1089
SsiI CCGC 2 cut(s) 334, 617
SspMI CTAG 1 cut(s) 1098
SstI GAGCTC 2 cut(s) 93, 192
StyD4I CCNGG 1 cut(s) 835
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 3 cut(s) 469, 910, 1005
TaqI TCGA 4 cut(s) 638, 711, 721, 993
TasI AATT 5 cut(s) 262, 326, 682, 707, 1089
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 3 cut(s) 497, 625, 715
Tru1I TTAA 2 cut(s) 1050, 1088
Tru9I TTAA 2 cut(s) 1050, 1088
TscAI CASTG 2 cut(s) 514, 925
TseI GCWGC 2 cut(s) 378, 812
TspDTI ATGAA 4 cut(s) 527, 747, 752, 957
TspGWI ACGGA 1 cut(s) 237
TspRI CASTG 2 cut(s) 514, 925
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 2 cut(s) 682, 707
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 1 cut(s) 1098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.