Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002853

Physical Location & Seq

Genomic Coordinates Forward (+)

25167350 .. 25169778

Gene Structure 2429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033275

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1806 bp

ATGGCTAATCTATTGCCTCTCCTCTTCTTCCTCCTCCTCCAACCTCTCTTCTCCATTGCCAATGTCCACAAACTCAACCAGCTCAGATCAGAGCTCATCTCCCAATCCACTTCTCTCAATGCCACCTCACCCACCCTTTACTTTGAAGTAACCCAACCCATTCCAGTACCCCCAACCAAGCCTTGTTCTGAGCTCATTCTCCAACATGACTTTGCCTACACTTATGGCAAACCACCAGTCTTTGCTAACTACTCTCCTCCCTCAAATTGCCCATCTCAGAACTTCTCCAAGATTGTTCTTGAATGGAAAGCTACAAGCAAAGGAAGACAATTTGACCGAATTTTTGGGGTTTGGCTTTCTGGGGTTGAGCTTCTCAGGAGCTGCACAGCCGAGCCAAGGACCAATGGGATTGTCTGGAGTGTTGAGAAGGACATCACCAGGTACTATTCTTTGCTCCAAAAGAACCAGACACTTGCTGTTTATCTTGGCAATTTGGTTGATTCAACATATACTGGAGTTTACCATGTGAATATAAGTTTTCATTTTTATCCTGCTGAGGCGAGTTTGCATAGTTATGATCAAAATGTGGGGGATTTGGCATCTGGGCACCATTCTTGGGCGGATTTGATTCTGCCCATTTCGAGAAATCTGCCTTTGACTGATGGGTTGTGGTTTGAAGTTGAGAATTCAACTGATACCGAGTTGAAGGAATTTGAGATTCCTCGAAATGCTTATAGGGCTGTGTTGGAGGTTTATGTTTCATTTCATGAGAATGATGAGTTTTGGTATTCCAATCCTACTAATGAGTATATTGCTGCCAACAACCTTACTGGCACGCCCGGAAATGGGCCTTTTAGGGAGGTTGTGGTGAGCTTAGATGGTGTGGTTGTTGGTGCTGTATGGCCTTTTACTGTGGTTTACACTGGAGGGATCAATCCTCTTCTGTGGAGACCCATTACCGGAATTGGCTCATTTGATCTACCTTCTTATGATATCGAAATCACACCGTTTTTAGGGAACATATTGGATGGGAAGAGCCACAAGATTGGGTTTAATGTTACCAATGCCTTGAATGTGTGGTACATTGATGCAAATCTGCATATTTGGTTGGACAAACAGAGCACAAGAACTCAAGCACAGCTTGTGAAGCATACTAGCTTGCCCCTTATTGTTTCAGTGGTGTCGGATTTTGTGGGTTTAAATGGCACATTTGTGACAAGGGTTAGCAGGTCAGTCTCATCAACTGGGTGGGTGAAGTCCTCTAATGGGAATATCACAACCCAGTCAATTCAAGATTTTTCCTACCTTAATAAAATGGTCATGGGGAATGATGGCAATTTGCAGACAGTGGACCAGACCATAAATTTCAATGATACCGTTCTTGTTAAGACGCCACCTTCCTATGTTCATTCACTGAAATCAAACAAATCATTTCTCCTTTCCATGTACACTGACTTGTTGGAACAAGAAGATGGAACTTATTTATATGTGACAAACGTTGATTTGGGATTCATTGATAAGAAATCTTCAAGCGGTGGTTCAAGACTCTTGGAGAGCACTCTCAGAAATTTGCAGAATGCAGAGGGTAATATGGTTGTAAAAAACAATTTGGTAGTTAGCGGAGTGGGGAATACACAGGAAGTGTACAGATATCATGGTGGCAAATTCTGTTACTTCAGAAATGTCAGTAGCTCAAACTACACCATACTTTATGATGAAGTGGAGAATACATGCCACAAAAAGGCCAAGTCTAATTATGATTTTGTCTTGAGAAGACTGTGGCCTTTTGGTACTGGATTTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PNGaseA

Protein Analysis

602

Amino Acids

67.46

Weight (kDa)

5.98

Isoelectric Point (pI)

37.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PNGaseA	PF12222	60 - 384	5.4e-106	Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C	PF25156	403 - 480	9.3e-21	PNGase_A C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G14920
fragaria_vesca	FvH4_3g18311 FvH4_3g18320 FvH4_3g18320
malus_domestica	MD03G1242900.v1.1 MD03G1243300.v1.1 MD03G1243400.v1.1
prunus_persica	Prupe.4G164900_v2.0.a1
pyrus_communis	pycom11g23210
rosa_chinensis	RchiOBHm_Chr5g0030951 RchiOBHm_Chr5g0030961 RchiOBHm_Chr5g0030971
rosa_laevigata	RLG00000033274 RLG00000033275 RLG00000033276
rosa_multiflora	Rmu_sc0001974.1_g000004 Rmu_sc0001974.1_g000008 Rmu_sc0001974.1_g000009 Rmu_sc0040817.1_g000002
rosa_roxburghii	Rroxscaffold_1G00049310 Rroxscaffold_1G00049320
rosa_rugosa	Rorug05G0121600 Rorug05G0121700 Rorug05G0121900
rosa_samantha	Rh5AG214900 Rh5AG215000 Rh5AG215200 Rh5BG213900 Rh5BG214000 Rh5BG214200 Rh5CG237800 Rh5CG237900 Rh5CG238100 Rh5DG218500 Rh5DG218600 Rh5DG218800
rosa_wichuraiana	Rw5G019660 Rw5G019670 Rw5G019680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1218
AccB1I	GGYRCC	1 cut(s)	606
AciI	CCGC	3 cut(s)	620, 1533, 1620
AclI	AACGTT	1 cut(s)	1497
AclWI	GGATC	1 cut(s)	938
AcsI	RAATTY	6 cut(s)	339, 685, 710, 1363, 1567, 1664
AcuI	CTGAAG	1 cut(s)	1660
AcyI	GRCGYC	1 cut(s)	1391
AfaI	GTAC	6 cut(s)	168, 443, 1082, 1448, 1646, 1792
AfiI	CCNNNNNNNGG	8 cut(s)	396, 616, 845, 846, 959, 1013, 1266, 1741
AjnI	CCWGG	1 cut(s)	437
AleI	CACNNNNGTG	1 cut(s)	1211
Alw21I	GWGCWC	4 cut(s)	96, 195, 1124, 1559
Alw26I	GTCTC	2 cut(s)	943, 1240
AlwI	GGATC	1 cut(s)	938
AlwNI	CAGNNNCTG	1 cut(s)	381
AoxI	GGCC	4 cut(s)	848, 902, 1743, 1781
ApeKI	GCWGC	2 cut(s)	381, 815
ApoI	RAATTY	6 cut(s)	339, 685, 710, 1363, 1567, 1664
ArsI	GACNNNNNNTTYG	4 cut(s)	222, 254, 326, 358
AspS9I	GGNCC	3 cut(s)	399, 848, 1351
AsuC2I	CCSGG	1 cut(s)	840
AsuHPI	GGTGA	4 cut(s)	120, 427, 880, 1264
AvaII	GGWCC	2 cut(s)	399, 1351
BaeGI	GKGCMC	1 cut(s)	609
BanI	GGYRCC	1 cut(s)	606
BanII	GRGCYC	2 cut(s)	96, 195
BbsI	GAAGAC	2 cut(s)	331, 1780
Bbv12I	GWGCWC	4 cut(s)	96, 195, 1124, 1559
BbvCI	CCTCAGC	1 cut(s)	555
BbvI	GCAGC	2 cut(s)	368, 802
BccI	CCATC	6 cut(s)	280, 656, 872, 1022, 1325, 1466
BcgI	CGANNNNNNTGC	2 cut(s)	631, 665
BciT130I	CCWGG	1 cut(s)	439
BclI	TGATCA	1 cut(s)	577
BcnI	CCSGG	1 cut(s)	840
BcoDI	GTCTC	2 cut(s)	943, 1240
BfaI	CTAG	1 cut(s)	1155
BfuAI	ACCTGC	1 cut(s)	1218
BisI	GCNGC	2 cut(s)	382, 816
BlsI	GCNGC	2 cut(s)	383, 817
Bme1390I	CCNGG	2 cut(s)	439, 840
Bme18I	GGWCC	2 cut(s)	399, 1351
BmgT120I	GGNCC	3 cut(s)	399, 848, 1351
BmiI	GGNNCC	1 cut(s)	608
BmrFI	CCNGG	2 cut(s)	439, 840
BmrI	ACTGGG	2 cut(s)	1254, 1276
BmsI	GCATC	2 cut(s)	608, 1078
BmuI	ACTGGG	2 cut(s)	1254, 1276
BpiI	GAAGAC	2 cut(s)	331, 1780
BplI	GAGNNNNNCTC	2 cut(s)	83, 115
BpmI	CTGGAG	3 cut(s)	436, 534, 945
Bpu10I	CCTNAGC	1 cut(s)	555
BpuEI	CTTGAG	2 cut(s)	1116, 1789
BpuMI	CCSGG	1 cut(s)	840
BsaBI	GATNNNNATC	1 cut(s)	1092
BsaHI	GRCGYC	1 cut(s)	1391
BsaI	GGTCTC	1 cut(s)	943
BsaJI	CCNNGG	1 cut(s)	395
BsaWI	WCCGGW	1 cut(s)	959
Bsc4I	CCNNNNNNNGG	8 cut(s)	396, 616, 845, 846, 959, 1013, 1266, 1741
Bse1I	ACTGG	8 cut(s)	164, 236, 517, 835, 928, 1249, 1282, 1798
Bse3DI	GCAATG	1 cut(s)	54
Bse8I	GATNNNNATC	1 cut(s)	1092
BseBI	CCWGG	1 cut(s)	439
BseDI	CCNNGG	1 cut(s)	395
BseGI	GGATG	1 cut(s)	1033
BseJI	GATNNNNATC	1 cut(s)	1092
BseLI	CCNNNNNNNGG	8 cut(s)	396, 616, 845, 846, 959, 1013, 1266, 1741
BseMI	GCAATG	1 cut(s)	54
BseMII	CTCAG	6 cut(s)	97, 180, 290, 388, 546, 1576
BseNI	ACTGG	8 cut(s)	164, 236, 517, 835, 928, 1249, 1282, 1798
BseRI	GAGGAG	4 cut(s)	11, 23, 26, 246
BseSI	GKGCMC	1 cut(s)	609
BseXI	GCAGC	2 cut(s)	368, 802
BsgI	GTGCAG	1 cut(s)	367
BshFI	GGCC	4 cut(s)	850, 904, 1745, 1783
BshNI	GGYRCC	1 cut(s)	606
BsiHKAI	GWGCWC	4 cut(s)	96, 195, 1124, 1559
BsiSI	CCGG	2 cut(s)	840, 960
BslI	CCNNNNNNNGG	8 cut(s)	396, 616, 845, 846, 959, 1013, 1266, 1741
BsmAI	GTCTC	2 cut(s)	943, 1240
BsmI	GAATGC	1 cut(s)	1582
BsnI	GGCC	4 cut(s)	850, 904, 1745, 1783
Bso31I	GGTCTC	1 cut(s)	943
Bsp1286I	GDGCHC	5 cut(s)	96, 195, 609, 1124, 1559
Bsp1407I	TGTACA	2 cut(s)	1446, 1644
Bsp143I	GATC	4 cut(s)	86, 577, 930, 976
BspACI	CCGC	3 cut(s)	620, 1533, 1620
BspANI	GGCC	4 cut(s)	850, 904, 1745, 1783
BspCNI	CTCAG	6 cut(s)	96, 181, 289, 387, 547, 1575
BspHI	TCATGA	1 cut(s)	766
BspLI	GGNNCC	1 cut(s)	608
BspMI	ACCTGC	1 cut(s)	1218
BspPI	GGATC	1 cut(s)	938
BspQI	GCTCTTC	1 cut(s)	1028
BspT107I	GGYRCC	1 cut(s)	606
BspTNI	GGTCTC	1 cut(s)	943
BsrDI	GCAATG	1 cut(s)	54
BsrGI	TGTACA	2 cut(s)	1446, 1644
BsrI	ACTGG	8 cut(s)	164, 236, 517, 835, 928, 1249, 1282, 1798
BssECI	CCNNGG	1 cut(s)	395
BssMI	GATC	4 cut(s)	86, 577, 930, 976
BssNI	GRCGYC	1 cut(s)	1391
BssT1I	CCWWGG	1 cut(s)	395
Bst2UI	CCWGG	1 cut(s)	439
Bst4CI	ACNGT	5 cut(s)	913, 1008, 1348, 1378, 1779
Bst6I	CTCTTC	4 cut(s)	29, 53, 945, 1028
BstACI	GRCGYC	1 cut(s)	1391
BstAUI	TGTACA	2 cut(s)	1446, 1644
BstC8I	GCNNGC	2 cut(s)	836, 1160
BstDEI	CTNAG	7 cut(s)	83, 189, 276, 374, 555, 874, 1562
BstF5I	GGATG	1 cut(s)	1033
BstKTI	GATC	4 cut(s)	89, 580, 933, 979
BstMAI	GTCTC	2 cut(s)	943, 1240
BstMBI	GATC	4 cut(s)	86, 577, 930, 976
BstMWI	GCNNNNNNNGC	2 cut(s)	737, 1147
BstNI	CCWGG	1 cut(s)	439
BstNSI	RCATGY	1 cut(s)	1734
BstSCI	CCNGG	2 cut(s)	437, 838
BstSLI	GKGCMC	1 cut(s)	609
BstV1I	GCAGC	2 cut(s)	368, 802
BstV2I	GAAGAC	2 cut(s)	331, 1780
BstXI	CCANNNNNNTGG	1 cut(s)	1046
BsuRI	GGCC	4 cut(s)	850, 904, 1745, 1783
BtsCI	GGATG	1 cut(s)	1033
BtsIMutI	CAGTG	5 cut(s)	921, 1182, 1353, 1412, 1449
BveI	ACCTGC	1 cut(s)	1218
Cac8I	GCNNGC	2 cut(s)	836, 1160
CaiI	CAGNNNCTG	1 cut(s)	381
CciI	TCATGA	1 cut(s)	766
Cfr13I	GGNCC	3 cut(s)	399, 848, 1351
CseI	GACGC	1 cut(s)	1399
CsiI	ACCWGGT	1 cut(s)	437
Csp6I	GTAC	6 cut(s)	167, 442, 1081, 1447, 1645, 1791
CviAII	CATG	7 cut(s)	206, 524, 767, 1321, 1444, 1655, 1731
CviQI	GTAC	6 cut(s)	167, 442, 1081, 1447, 1645, 1791
DdeI	CTNAG	7 cut(s)	83, 189, 276, 374, 555, 874, 1562
DpnI	GATC	4 cut(s)	88, 579, 932, 978
DpnII	GATC	4 cut(s)	86, 577, 930, 976
DraI	TTTAAA	1 cut(s)	1200
Eam1104I	CTCTTC	4 cut(s)	29, 53, 945, 1028
EarI	CTCTTC	4 cut(s)	29, 53, 945, 1028
EciI	GGCGGA	1 cut(s)	635
Ecl136II	GAGCTC	2 cut(s)	94, 193
Eco130I	CCWWGG	1 cut(s)	395
Eco24I	GRGCYC	2 cut(s)	96, 195
Eco31I	GGTCTC	1 cut(s)	943
Eco32I	GATATC	2 cut(s)	994, 1652
Eco47I	GGWCC	2 cut(s)	399, 1351
Eco53kI	GAGCTC	2 cut(s)	94, 193
Eco57I	CTGAAG	1 cut(s)	1660
EcoICRI	GAGCTC	2 cut(s)	94, 193
EcoRI	GAATTC	1 cut(s)	685
EcoRII	CCWGG	1 cut(s)	437
EcoRV	GATATC	2 cut(s)	994, 1652
EcoT14I	CCWWGG	1 cut(s)	395
EcoT38I	GRGCYC	2 cut(s)	96, 195
ErhI	CCWWGG	1 cut(s)	395
FaeI	CATG	7 cut(s)	209, 527, 770, 1324, 1447, 1658, 1734
FalI	AAGNNNNNCTT	2 cut(s)	1125, 1157
FatI	CATG	7 cut(s)	205, 523, 766, 1320, 1443, 1654, 1730
FbaI	TGATCA	1 cut(s)	577
Fnu4HI	GCNGC	2 cut(s)	382, 816
FokI	GGATG	1 cut(s)	1040
FriOI	GRGCYC	2 cut(s)	96, 195
Fsp4HI	GCNGC	2 cut(s)	382, 816
FspBI	CTAG	1 cut(s)	1155
GluI	GCNGC	2 cut(s)	382, 816
GsuI	CTGGAG	3 cut(s)	436, 534, 945
HaeIII	GGCC	4 cut(s)	850, 904, 1745, 1783
HapII	CCGG	2 cut(s)	840, 960
HgaI	GACGC	1 cut(s)	1399
Hin1I	GRCGYC	1 cut(s)	1391
Hin1II	CATG	7 cut(s)	209, 527, 770, 1324, 1447, 1658, 1734
HinfI	GANTC	5 cut(s)	500, 628, 718, 1509, 1545
HpaII	CCGG	2 cut(s)	840, 960
HphI	GGTGA	4 cut(s)	120, 427, 880, 1264
Hpy166II	GTNNAC	6 cut(s)	67, 520, 919, 1351, 1449, 1645
Hpy188I	TCNGA	7 cut(s)	86, 91, 190, 279, 1186, 1565, 1679
Hpy188III	TCNNGA	8 cut(s)	299, 376, 415, 642, 767, 1292, 1542, 1768
Hpy8I	GTNNAC	6 cut(s)	67, 520, 919, 1351, 1449, 1645
HpyAV	CCTTC	4 cut(s)	421, 700, 993, 1407
HpyCH4III	ACNGT	5 cut(s)	913, 1008, 1348, 1378, 1779
HpyCH4IV	ACGT	1 cut(s)	1497
HpyCH4V	TGCA	7 cut(s)	384, 568, 1091, 1099, 1342, 1573, 1580
HpyF10VI	GCNNNNNNNGC	2 cut(s)	737, 1147
HpyF3I	CTNAG	7 cut(s)	83, 189, 276, 374, 555, 874, 1562
HpySE526I	ACGT	1 cut(s)	1497
Hsp92I	GRCGYC	1 cut(s)	1391
Hsp92II	CATG	7 cut(s)	209, 527, 770, 1324, 1447, 1658, 1734
Ksp22I	TGATCA	1 cut(s)	577
Kzo9I	GATC	4 cut(s)	86, 577, 930, 976
LguI	GCTCTTC	1 cut(s)	1028
LmnI	GCTCC	2 cut(s)	378, 459
Lsp1109I	GCAGC	2 cut(s)	368, 802
LweI	GCATC	2 cut(s)	608, 1078
MabI	ACCWGGT	1 cut(s)	437
MaeI	CTAG	1 cut(s)	1155
MaeII	ACGT	1 cut(s)	1497
MaeIII	GTNAC	5 cut(s)	148, 1057, 1213, 1489, 1670
MalI	GATC	4 cut(s)	88, 579, 932, 978
MboI	GATC	4 cut(s)	86, 577, 930, 976
MboII	GAAGA	9 cut(s)	16, 19, 40, 336, 932, 1045, 1481, 1518, 1785
MhlI	GDGCHC	5 cut(s)	96, 195, 609, 1124, 1559
MlyI	GAGTC	1 cut(s)	1539
MmeI	TCCRAC	6 cut(s)	64, 226, 726, 1089, 1164, 1440
MseI	TTAA	4 cut(s)	1053, 1199, 1308, 1386
MslI	CAYNNNNRTG	3 cut(s)	573, 771, 1211
MspI	CCGG	2 cut(s)	840, 960
MspR9I	CCNGG	2 cut(s)	439, 840
Mva1269I	GAATGC	1 cut(s)	1582
MvaI	CCWGG	1 cut(s)	439
MwoI	GCNNNNNNNGC	2 cut(s)	737, 1147
NciI	CCSGG	1 cut(s)	840
NdeII	GATC	4 cut(s)	86, 577, 930, 976
NlaIII	CATG	7 cut(s)	209, 527, 770, 1324, 1447, 1658, 1734
NlaIV	GGNNCC	1 cut(s)	608
NmeAIII	GCCGAG	1 cut(s)	415
NmuCI	GTSAC	2 cut(s)	1213, 1489
NspI	RCATGY	1 cut(s)	1734
OliI	CACNNNNGTG	1 cut(s)	1211
PagI	TCATGA	1 cut(s)	766
PciSI	GCTCTTC	1 cut(s)	1028
PctI	GAATGC	1 cut(s)	1582
PfeI	GAWTC	4 cut(s)	500, 628, 718, 1509
PkrI	GCNGC	2 cut(s)	383, 817
PleI	GAGTC	1 cut(s)	1539
PpsI	GAGTC	1 cut(s)	1539
Psp124BI	GAGCTC	2 cut(s)	96, 195
Psp1406I	AACGTT	1 cut(s)	1497
Psp6I	CCWGG	1 cut(s)	437
PspGI	CCWGG	1 cut(s)	437
PspN4I	GGNNCC	1 cut(s)	608
PspPI	GGNCC	3 cut(s)	399, 848, 1351
PstNI	CAGNNNCTG	1 cut(s)	381
RsaI	GTAC	6 cut(s)	168, 443, 1082, 1448, 1646, 1792
RsaNI	GTAC	6 cut(s)	167, 442, 1081, 1447, 1645, 1791
RseI	CAYNNNNRTG	3 cut(s)	573, 771, 1211
SacI	GAGCTC	2 cut(s)	96, 195
SapI	GCTCTTC	1 cut(s)	1028
SaqAI	TTAA	4 cut(s)	1053, 1199, 1308, 1386
SatI	GCNGC	2 cut(s)	382, 816
Sau3AI	GATC	4 cut(s)	86, 577, 930, 976
Sau96I	GGNCC	3 cut(s)	399, 848, 1351
SchI	GAGTC	1 cut(s)	1539
ScrFI	CCNGG	2 cut(s)	439, 840
SduI	GDGCHC	5 cut(s)	96, 195, 609, 1124, 1559
SexAI	ACCWGGT	1 cut(s)	437
SfaNI	GCATC	2 cut(s)	608, 1078
SinI	GGWCC	2 cut(s)	399, 1351
SmiMI	CAYNNNNRTG	3 cut(s)	573, 771, 1211
SmlI	CTYRAG	2 cut(s)	1131, 1768
SmoI	CTYRAG	2 cut(s)	1131, 1768
SsiI	CCGC	3 cut(s)	620, 1533, 1620
SspMI	CTAG	1 cut(s)	1155
SstI	GAGCTC	2 cut(s)	96, 195
StyD4I	CCNGG	2 cut(s)	437, 838
StyI	CCWWGG	1 cut(s)	395
TaaI	ACNGT	5 cut(s)	913, 1008, 1348, 1378, 1779
TaiI	ACGT	1 cut(s)	1500
TaqI	TCGA	3 cut(s)	641, 724, 996
TaqII	GACCGA	1 cut(s)	351
TatI	WGTACW	2 cut(s)	1446, 1644
TfiI	GAWTC	4 cut(s)	500, 628, 718, 1509
Tru1I	TTAA	4 cut(s)	1053, 1199, 1308, 1386
Tru9I	TTAA	4 cut(s)	1053, 1199, 1308, 1386
TscAI	CASTG	5 cut(s)	928, 1182, 1353, 1419, 1456
TseFI	GTSAC	2 cut(s)	1213, 1489
TseI	GCWGC	2 cut(s)	381, 815
Tsp45I	GTSAC	2 cut(s)	1213, 1489
TspDTI	ATGAA	6 cut(s)	530, 750, 755, 1397, 1501, 1731
TspRI	CASTG	5 cut(s)	928, 1182, 1353, 1419, 1456
VpaK11BI	GGWCC	2 cut(s)	399, 1351
XapI	RAATTY	6 cut(s)	339, 685, 710, 1363, 1567, 1664
XceI	RCATGY	1 cut(s)	1734
XspI	CTAG	1 cut(s)	1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.