MD03G1247700.v1.1

salt tolerance-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015021
Physical Location & Seq
Forward (+)
33499410 .. 33501225
1816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1247700.v1.1.491

Sequence Viewer

Length: 897 bp
ATGAAGATACAGTGCAACGTCTGCGAGGCAGCGGAGGCGAACGTCCTCTGCTGCGCCGATGAGGCGGCTCTCTGCAGGCCGTGCGACGAGAAGGTTCACAAAGCGAACAAGCTCGCGAGCAAGCACCAGAGGGTTCCTTTGTCCTCCTCCCACATGCCCAAATGCGATATCTGCCAGGAGGCAGTTGGGTACTTTTTCTGCTTAGAAGATCGAGCTTTGCTCTGTAGAAAATGTGATGTTGCCGTTCACACGGCTAATAGCTTCGTGTCAGCTCACCGGAGGTTTTTGCTTACCGGAATCAAGGTTGGGCCTGAGCCGGCTCAGCCGGATTTCGGCGACGGTGATGGGGTTGGTGTTGGTGTTGGTGTTGGTGCTGATGCGTCTTCTTCTACTATGAAGTCACGTCCTGGGTCTGGTTCTGGCTCAGTGTCTAGATGTGAAACGCAGGACTCGTTGCCGGTGGAGTGCAAGGCGGCGCTGCCCGGAGTTGCCGGGGGGATGTCGTTTACTGGGGGTTCTGCAGCTGGGACTGTTCCACAGTGTTATATAGATGAGTTTCTTGGACTCTCTGATCTTGATCAGAGCTTTGGCTACATTGATAATGGATCTTCAAAGGCTGACTGCGGTAAACTTGGGGAATATGGCTCACCAGTTTTAAGATCATCTGAGGAGGAACTGGACGATTATGAGTGCATAGGTGAGGTGCCGGAGGCCTCATGGATGGTGCCACAGATACCTTCCCCACCTACAGCATCAGGACTATACTGGCCAAAAAGTCTTCAGAATCCGTCTGATTCTTCTACAATGTTTGTTCCAGACATCTGCCACTCACAAATGGAAAACCCTTTTTGTTCCCAACATAATGGTACTGTCTCAAAACATCGTAGGCAGTTCTAG

Protein Analysis

299

Amino Acids

31.69

Weight (kDa)

5.09

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 52 - 91 8.8e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10240
fragaria_vesca FvH4_3g17750
malus_domestica MD03G1247700.v1.1 MD11G1269800.v1.1
prunus_persica Prupe.4G156600_v2.0.a1
pyrus_communis pycom03g19640 pycom11g23830
rosa_chinensis RchiOBHm_Chr5g0029541
rosa_laevigata RLG00000033177
rosa_multiflora Rmu_sc0000210.1_g000014
rosa_roxburghii Rroxscaffold_1G00050400
rosa_samantha Rh5AG206900 Rh5BG205600 Rh5CG227700 Rh5DG209300
rosa_wichuraiana Rw5G018820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 703, 724
AccII CGCG 1 cut(s) 116
AciI CCGC 4 cut(s) 32, 65, 473, 624
AclWI GGATC 1 cut(s) 613
AcoI YGGCCR 1 cut(s) 767
AcuI CTGAAG 1 cut(s) 764
AfaI GTAC 2 cut(s) 191, 868
AfiI CCNNNNNNNGG 2 cut(s) 332, 413
AgsI TTSAA 1 cut(s) 612
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 2 cut(s) 174, 406
AluBI AGCT 6 cut(s) 112, 215, 261, 272, 524, 585
AluI AGCT 6 cut(s) 112, 215, 261, 272, 524, 585
Alw26I GTCTC 1 cut(s) 877
AlwI GGATC 1 cut(s) 613
AoxI GGCC 4 cut(s) 77, 308, 711, 767
ApeKI GCWGC 4 cut(s) 29, 51, 478, 521
AspLEI GCGC 2 cut(s) 56, 478
AspS9I GGNCC 1 cut(s) 308
AsuC2I CCSGG 2 cut(s) 483, 493
AsuHPI GGTGA 4 cut(s) 266, 353, 639, 710
BalI TGGCCA 1 cut(s) 769
BanI GGYRCC 2 cut(s) 703, 724
BbsI GAAGAC 2 cut(s) 375, 770
BbvI GCAGC 4 cut(s) 38, 41, 465, 533
BccI CCATC 2 cut(s) 338, 715
BceAI ACGGC 3 cut(s) 64, 227, 267
BciT130I CCWGG 2 cut(s) 176, 408
BclI TGATCA 1 cut(s) 577
BcnI CCSGG 2 cut(s) 483, 493
BcoDI GTCTC 1 cut(s) 877
BfaI CTAG 2 cut(s) 432, 895
BfmI CTRYAG 4 cut(s) 73, 223, 519, 747
BfoI RGCGCY 1 cut(s) 479
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 6 cut(s) 30, 52, 66, 474, 479, 522
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 6 cut(s) 31, 53, 67, 475, 480, 523
Bme1390I CCNGG 4 cut(s) 176, 408, 483, 493
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 308
BmiI GGNNCC 3 cut(s) 135, 705, 726
BmrFI CCNGG 4 cut(s) 176, 408, 483, 493
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 367, 761
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 2 cut(s) 375, 770
BplI GAGNNNNNCTC 2 cut(s) 204, 236
Bpu10I CCTNAGC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 483, 493
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 407, 492
BsaWI WCCGGW 2 cut(s) 276, 293
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 413
Bse118I RCCGGY 2 cut(s) 316, 457
Bse1I ACTGG 4 cut(s) 514, 650, 681, 770
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 2 cut(s) 176, 408
BseDI CCNNGG 2 cut(s) 407, 492
BseGI GGATG 2 cut(s) 504, 726
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 332, 413
BseMII CTCAG 4 cut(s) 303, 335, 438, 657
BseNI ACTGG 4 cut(s) 514, 650, 681, 770
BseRI GAGGAG 2 cut(s) 136, 683
BseXI GCAGC 4 cut(s) 38, 41, 465, 533
BseYI CCCAGC 1 cut(s) 524
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 4 cut(s) 79, 310, 713, 769
BshNI GGYRCC 2 cut(s) 703, 724
BsiSI CCGG 8 cut(s) 277, 294, 317, 326, 458, 483, 492, 707
BslFI GGGAC 1 cut(s) 541
BslI CCNNNNNNNGG 2 cut(s) 332, 413
BsmAI GTCTC 1 cut(s) 877
BsmFI GGGAC 1 cut(s) 541
BsnI GGCC 4 cut(s) 79, 310, 713, 769
Bsp143I GATC 5 cut(s) 208, 571, 577, 605, 659
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp68I TCGCGA 1 cut(s) 116
BspACI CCGC 4 cut(s) 32, 65, 473, 624
BspANI GGCC 4 cut(s) 79, 310, 713, 769
BspCNI CTCAG 4 cut(s) 304, 334, 437, 658
BspFNI CGCG 1 cut(s) 116
BspLI GGNNCC 3 cut(s) 135, 705, 726
BspMAI CTGCAG 2 cut(s) 77, 523
BspPI GGATC 1 cut(s) 613
BspT107I GGYRCC 2 cut(s) 703, 724
BsrFI RCCGGY 2 cut(s) 316, 457
BsrI ACTGG 4 cut(s) 514, 650, 681, 770
BssAI RCCGGY 2 cut(s) 316, 457
BssECI CCNNGG 2 cut(s) 407, 492
BssMI GATC 5 cut(s) 208, 571, 577, 605, 659
Bst2UI CCWGG 2 cut(s) 176, 408
Bst4CI ACNGT 5 cut(s) 12, 341, 532, 540, 871
BstAPI GCANNNNNTGC 2 cut(s) 21, 81
BstC8I GCNNGC 5 cut(s) 77, 114, 118, 122, 318
BstDEI CTNAG 5 cut(s) 202, 312, 321, 424, 666
BstF5I GGATG 2 cut(s) 504, 726
BstFNI CGCG 1 cut(s) 116
BstH2I RGCGCY 1 cut(s) 479
BstHHI GCGC 2 cut(s) 56, 478
BstKTI GATC 5 cut(s) 211, 574, 580, 608, 662
BstMAI GTCTC 1 cut(s) 877
BstMBI GATC 5 cut(s) 208, 571, 577, 605, 659
BstMWI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
BstNI CCWGG 2 cut(s) 176, 408
BstNSI RCATGY 1 cut(s) 157
BstSCI CCNGG 4 cut(s) 174, 406, 481, 491
BstSFI CTRYAG 4 cut(s) 73, 223, 519, 747
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 4 cut(s) 38, 41, 465, 533
BstV2I GAAGAC 2 cut(s) 375, 770
BstX2I RGATCY 1 cut(s) 605
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 1 cut(s) 605
BsuRI GGCC 4 cut(s) 79, 310, 713, 769
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 2 cut(s) 504, 726
BtsIMutI CAGTG 3 cut(s) 17, 432, 545
BtuMI TCGCGA 1 cut(s) 116
Cac8I GCNNGC 5 cut(s) 77, 114, 118, 122, 318
CfoI GCGC 2 cut(s) 56, 478
Cfr10I RCCGGY 2 cut(s) 316, 457
Cfr13I GGNCC 1 cut(s) 308
CseI GACGC 1 cut(s) 369
Csp6I GTAC 2 cut(s) 190, 867
CviAII CATG 2 cut(s) 154, 717
CviQI GTAC 2 cut(s) 190, 867
DdeI CTNAG 5 cut(s) 202, 312, 321, 424, 666
DpnI GATC 5 cut(s) 210, 573, 579, 607, 661
DpnII GATC 5 cut(s) 208, 571, 577, 605, 659
EaeI YGGCCR 1 cut(s) 767
Eco147I AGGCCT 1 cut(s) 713
Eco32I GATATC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 174, 406
EcoRV GATATC 1 cut(s) 169
FaeI CATG 2 cut(s) 157, 720
FaqI GGGAC 1 cut(s) 541
FatI CATG 2 cut(s) 153, 716
FbaI TGATCA 1 cut(s) 577
Fnu4HI GCNGC 6 cut(s) 30, 52, 66, 474, 479, 522
FokI GGATG 2 cut(s) 511, 733
Fsp4HI GCNGC 6 cut(s) 30, 52, 66, 474, 479, 522
FspBI CTAG 2 cut(s) 432, 895
GlaI GCGC 2 cut(s) 55, 477
GluI GCNGC 6 cut(s) 30, 52, 66, 474, 479, 522
GsaI CCCAGC 1 cut(s) 528
HaeII RGCGCY 1 cut(s) 479
HaeIII GGCC 4 cut(s) 79, 310, 713, 769
HapII CCGG 8 cut(s) 277, 294, 317, 326, 458, 483, 492, 707
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 56, 478
Hin1II CATG 2 cut(s) 157, 720
Hin6I GCGC 2 cut(s) 54, 476
HinP1I GCGC 2 cut(s) 54, 476
HinfI GANTC 5 cut(s) 297, 449, 564, 784, 794
HpaII CCGG 8 cut(s) 277, 294, 317, 326, 458, 483, 492, 707
HphI GGTGA 4 cut(s) 266, 353, 639, 710
Hpy166II GTNNAC 4 cut(s) 97, 247, 507, 629
Hpy188I TCNGA 5 cut(s) 571, 582, 667, 783, 793
Hpy188III TCNNGA 5 cut(s) 115, 432, 575, 756, 815
Hpy8I GTNNAC 4 cut(s) 97, 247, 507, 629
Hpy99I CGWCG 2 cut(s) 89, 341
HpyAV CCTTC 2 cut(s) 85, 747
HpyCH4III ACNGT 5 cut(s) 12, 341, 532, 540, 871
HpyCH4IV ACGT 3 cut(s) 18, 42, 403
HpyCH4V TGCA 5 cut(s) 15, 75, 468, 521, 693
HpyF10VI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
HpyF3I CTNAG 5 cut(s) 202, 312, 321, 424, 666
HpySE526I ACGT 3 cut(s) 18, 42, 403
Hsp92II CATG 2 cut(s) 157, 720
HspAI GCGC 2 cut(s) 54, 476
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 577
Kzo9I GATC 5 cut(s) 208, 571, 577, 605, 659
Lsp1109I GCAGC 4 cut(s) 38, 41, 465, 533
LweI GCATC 2 cut(s) 367, 761
MaeI CTAG 2 cut(s) 432, 895
MaeII ACGT 3 cut(s) 18, 42, 403
MaeIII GTNAC 1 cut(s) 399
MalI GATC 5 cut(s) 210, 573, 579, 607, 661
MboI GATC 5 cut(s) 208, 571, 577, 605, 659
MboII GAAGA 7 cut(s) 16, 218, 375, 378, 600, 770, 789
MflI RGATCY 1 cut(s) 605
MlsI TGGCCA 1 cut(s) 769
MluNI TGGCCA 1 cut(s) 769
MlyI GAGTC 2 cut(s) 443, 558
Mox20I TGGCCA 1 cut(s) 769
MroNI GCCGGC 1 cut(s) 316
MscI TGGCCA 1 cut(s) 769
MseI TTAA 1 cut(s) 656
Msp20I TGGCCA 1 cut(s) 769
MspA1I CMGCKG 2 cut(s) 32, 524
MspI CCGG 8 cut(s) 277, 294, 317, 326, 458, 483, 492, 707
MspR9I CCNGG 4 cut(s) 176, 408, 483, 493
MvaI CCWGG 2 cut(s) 176, 408
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 6 cut(s) 21, 35, 62, 81, 171, 322
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 2 cut(s) 483, 493
NdeII GATC 5 cut(s) 208, 571, 577, 605, 659
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 157, 720
NlaIV GGNNCC 3 cut(s) 135, 705, 726
NmuCI GTSAC 1 cut(s) 399
NruI TCGCGA 1 cut(s) 116
NspI RCATGY 1 cut(s) 157
PceI AGGCCT 1 cut(s) 713
PcsI WCGNNNNNNNCGW 1 cut(s) 449
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 3 cut(s) 297, 784, 794
PkrI GCNGC 6 cut(s) 31, 53, 67, 475, 480, 523
PleI GAGTC 2 cut(s) 443, 558
PpsI GAGTC 2 cut(s) 443, 558
Psp6I CCWGG 2 cut(s) 174, 406
PspFI CCCAGC 1 cut(s) 524
PspGI CCWGG 2 cut(s) 174, 406
PspN4I GGNNCC 3 cut(s) 135, 705, 726
PspPI GGNCC 1 cut(s) 308
PstI CTGCAG 2 cut(s) 77, 523
PsuI RGATCY 1 cut(s) 605
PvuII CAGCTG 1 cut(s) 524
RruI TCGCGA 1 cut(s) 116
RsaI GTAC 2 cut(s) 191, 868
RsaNI GTAC 2 cut(s) 190, 867
SaqAI TTAA 1 cut(s) 656
SatI GCNGC 6 cut(s) 30, 52, 66, 474, 479, 522
Sau3AI GATC 5 cut(s) 208, 571, 577, 605, 659
Sau96I GGNCC 1 cut(s) 308
SchI GAGTC 2 cut(s) 443, 558
ScrFI CCNGG 4 cut(s) 176, 408, 483, 493
SfaNI GCATC 2 cut(s) 367, 761
SfcI CTRYAG 4 cut(s) 73, 223, 519, 747
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 4 cut(s) 32, 65, 473, 624
SspMI CTAG 2 cut(s) 432, 895
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 4 cut(s) 174, 406, 481, 491
TaaI ACNGT 5 cut(s) 12, 341, 532, 540, 871
TaiI ACGT 3 cut(s) 21, 45, 406
TaqI TCGA 1 cut(s) 211
TauI GCSGC 2 cut(s) 68, 476
TfiI GAWTC 3 cut(s) 297, 784, 794
Tru1I TTAA 1 cut(s) 656
Tru9I TTAA 1 cut(s) 656
TscAI CASTG 3 cut(s) 17, 432, 545
TseFI GTSAC 1 cut(s) 399
TseI GCWGC 4 cut(s) 29, 51, 478, 521
Tsp45I GTSAC 1 cut(s) 399
TspDTI ATGAA 2 cut(s) 17, 410
TspGWI ACGGA 1 cut(s) 777
TspRI CASTG 3 cut(s) 17, 432, 545
XbaI TCTAGA 1 cut(s) 431
XceI RCATGY 1 cut(s) 157
XcmI CCANNNNNNNNNTGG 1 cut(s) 182
XspI CTAG 2 cut(s) 432, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.