Rh5CG227700

salt tolerance-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0015021
Physical Location & Seq
Forward (+)
23267180 .. 23268415
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG227700.1

Sequence Viewer

Length: 705 bp
ATGCCCAAGTGCGATATCTGCCAGGAGGGGGTTGGGTATTTTTTCTGTTTGGAGGATAGAGCTCTGCTGTGTAGGAACTGTGATGTTGCTGTACATACTGCGAATAGTTTTGTTTCCGGTCACCGGAGGTTTTTGCTGACCGGAATTAAAGTTGGGCCGGAGTCCAATCCACCTGGGTCTGGTGGCGGTGATGGTGGTGTTGCTGGGTCGTCTTCTTCTGTCAAACCGGACCCTGGGGCTGCGAAGAAATGTGAAGCAAACATTCAATTGGCGGAGGAATGCAAAGTGGCGCCGTCAAGTGTTGCTGGGATGTCTTTTGCTGGTGGTTCTGCAGCTGGGTCTGTTCCACAGTGGCCTATAGATGAGTTTCTGGGATTCACTGATTTCGATCAGAGCTTTGGGTACATGGATAATGGATCGTCTAAGTCTGACTGCGGTAAACTTGGGGCTTCTGATTCATCAATTCTAAGATCATCTGAGGAGGAGCAAGAGGAATACGAGTGCATAGGTCAGGTGCCAGAGATCTCTTGGATGGTGCCAGAGGTACCTTCCCCGCCTACATCCTCGGGGCTATACTGGCCTAAAACTTATCAGAACCCATCGGATTCTGCAGTGTTTGTTCCAGACGTTTGCTATTCAGAAATGGAAAATCCTCTTCACTGTCAACAGAATGGTACTGTCTCAAAACGTCGTAGGCAATTCTAA

Protein Analysis

234

Amino Acids

24.97

Weight (kDa)

4.66

Isoelectric Point (pI)

67.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 2 - 42 6.1e-07 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10240
fragaria_vesca FvH4_3g17750
malus_domestica MD03G1247700.v1.1 MD11G1269800.v1.1
prunus_persica Prupe.4G156600_v2.0.a1
pyrus_communis pycom03g19640 pycom11g23830
rosa_chinensis RchiOBHm_Chr5g0029541
rosa_laevigata RLG00000033177
rosa_multiflora Rmu_sc0000210.1_g000014
rosa_roxburghii Rroxscaffold_1G00050400
rosa_samantha Rh5AG206900 Rh5BG205600 Rh5CG227700 Rh5DG209300
rosa_wichuraiana Rw5G018820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 544
AccB1I GGYRCC 4 cut(s) 289, 514, 535, 544
AciI CCGC 4 cut(s) 186, 272, 435, 554
AclWI GGATC 1 cut(s) 424
AcyI GRCGYC 1 cut(s) 290
AfaI GTAC 4 cut(s) 93, 404, 546, 676
AfiI CCNNNNNNNGG 4 cut(s) 28, 123, 179, 233
AgsI TTSAA 1 cut(s) 266
AjnI CCWGG 3 cut(s) 21, 172, 232
AluBI AGCT 3 cut(s) 62, 335, 396
AluI AGCT 3 cut(s) 62, 335, 396
Alw21I GWGCWC 1 cut(s) 64
Alw26I GTCTC 1 cut(s) 685
AlwI GGATC 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 565
AoxI GGCC 3 cut(s) 155, 353, 578
ApeKI GCWGC 2 cut(s) 239, 332
Asp718I GGTACC 1 cut(s) 544
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 2 cut(s) 155, 229
AsuHPI GGTGA 2 cut(s) 113, 200
AvaI CYCGRG 1 cut(s) 565
AvaII GGWCC 1 cut(s) 229
BanI GGYRCC 4 cut(s) 289, 514, 535, 544
BanII GRGCYC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 64
BbvI GCAGC 2 cut(s) 226, 344
BccI CCATC 3 cut(s) 185, 526, 607
BceAI ACGGC 1 cut(s) 277
BciT130I CCWGG 3 cut(s) 23, 174, 234
BcoDI GTCTC 1 cut(s) 685
BfmI CTRYAG 3 cut(s) 330, 357, 609
BfoI RGCGCY 1 cut(s) 293
BglII AGATCT 1 cut(s) 522
BisI GCNGC 2 cut(s) 240, 333
BlsI GCNGC 2 cut(s) 241, 334
Bme1390I CCNGG 3 cut(s) 23, 174, 234
Bme18I GGWCC 1 cut(s) 229
BmeT110I CYCGRG 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 155, 229
BmiI GGNNCC 5 cut(s) 231, 291, 516, 537, 546
BmrFI CCNGG 3 cut(s) 23, 174, 234
BpiI GAAGAC 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 290
BsaJI CCNNGG 4 cut(s) 173, 232, 233, 564
BsaWI WCCGGW 4 cut(s) 116, 123, 140, 226
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 123, 179, 233
Bse1I ACTGG 1 cut(s) 581
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 3 cut(s) 23, 174, 234
BseDI CCNNGG 4 cut(s) 173, 232, 233, 564
BseGI GGATG 3 cut(s) 315, 537, 560
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 4 cut(s) 28, 123, 179, 233
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 1 cut(s) 581
BseRI GAGGAG 2 cut(s) 494, 497
BseXI GCAGC 2 cut(s) 226, 344
BseYI CCCAGC 3 cut(s) 203, 305, 335
BshFI GGCC 3 cut(s) 157, 355, 580
BshNI GGYRCC 4 cut(s) 289, 514, 535, 544
BsiHKAI GWGCWC 1 cut(s) 64
BsiHKCI CYCGRG 1 cut(s) 565
BsiSI CCGG 5 cut(s) 117, 124, 141, 158, 227
BslI CCNNNNNNNGG 4 cut(s) 28, 123, 179, 233
BsmAI GTCTC 1 cut(s) 685
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 3 cut(s) 157, 355, 580
BsoBI CYCGRG 1 cut(s) 565
Bsp1286I GDGCHC 1 cut(s) 64
Bsp1407I TGTACA 1 cut(s) 91
Bsp143I GATC 4 cut(s) 388, 416, 470, 522
BspACI CCGC 4 cut(s) 186, 272, 435, 554
BspANI GGCC 3 cut(s) 157, 355, 580
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 5 cut(s) 231, 291, 516, 537, 546
BspMAI CTGCAG 2 cut(s) 334, 613
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 4 cut(s) 289, 514, 535, 544
BsrGI TGTACA 1 cut(s) 91
BsrI ACTGG 1 cut(s) 581
BssECI CCNNGG 4 cut(s) 173, 232, 233, 564
BssMI GATC 4 cut(s) 388, 416, 470, 522
BssNI GRCGYC 1 cut(s) 290
Bst2UI CCWGG 3 cut(s) 23, 174, 234
Bst4CI ACNGT 4 cut(s) 80, 351, 662, 679
Bst6I CTCTTC 1 cut(s) 660
BstACI GRCGYC 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 91
BstDEI CTNAG 3 cut(s) 423, 467, 477
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 3 cut(s) 315, 537, 560
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 4 cut(s) 391, 419, 473, 525
BstMAI GTCTC 1 cut(s) 685
BstMBI GATC 4 cut(s) 388, 416, 470, 522
BstMWI GCNNNNNNNGC 2 cut(s) 18, 577
BstNI CCWGG 3 cut(s) 23, 174, 234
BstPI GGTNACC 1 cut(s) 119
BstSCI CCNGG 3 cut(s) 21, 172, 232
BstSFI CTRYAG 3 cut(s) 330, 357, 609
BstV1I GCAGC 2 cut(s) 226, 344
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 522
BstYI RGATCY 1 cut(s) 522
BsuRI GGCC 3 cut(s) 157, 355, 580
BtsCI GGATG 3 cut(s) 315, 537, 560
BtsI GCAGTG 1 cut(s) 618
BtsIMutI CAGTG 4 cut(s) 356, 378, 618, 658
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 2 cut(s) 155, 229
Csp6I GTAC 4 cut(s) 92, 403, 545, 675
CviAII CATG 1 cut(s) 406
CviJI RGCY 9 cut(s) 62, 157, 239, 335, 355, 396, 449, 571, 580
CviKI_1 RGCY 9 cut(s) 62, 157, 239, 335, 355, 396, 449, 571, 580
CviQI GTAC 4 cut(s) 92, 403, 545, 675
DdeI CTNAG 3 cut(s) 423, 467, 477
DinI GGCGCC 1 cut(s) 291
DpnI GATC 4 cut(s) 390, 418, 472, 524
DpnII GATC 4 cut(s) 388, 416, 470, 522
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
EciI GGCGGA 1 cut(s) 287
Ecl136II GAGCTC 1 cut(s) 62
Eco24I GRGCYC 1 cut(s) 64
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 62
Eco88I CYCGRG 1 cut(s) 565
Eco91I GGTNACC 1 cut(s) 119
EcoICRI GAGCTC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 119
EcoRII CCWGG 3 cut(s) 21, 172, 232
EcoRV GATATC 1 cut(s) 16
EcoT38I GRGCYC 1 cut(s) 64
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
FaeI CATG 1 cut(s) 409
FaiI YATR 5 cut(s) 96, 359, 407, 506, 574
FatI CATG 1 cut(s) 405
FauI CCCGC 1 cut(s) 561
Fnu4HI GCNGC 2 cut(s) 240, 333
FokI GGATG 3 cut(s) 322, 544, 547
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 240, 333
GlaI GCGC 1 cut(s) 291
GluI GCNGC 2 cut(s) 240, 333
GsaI CCCAGC 3 cut(s) 207, 309, 339
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 3 cut(s) 157, 355, 580
HapII CCGG 5 cut(s) 117, 124, 141, 158, 227
HhaI GCGC 1 cut(s) 292
Hin1I GRCGYC 1 cut(s) 290
Hin1II CATG 1 cut(s) 409
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 665
HindII GTYRAC 1 cut(s) 665
HinfI GANTC 4 cut(s) 161, 375, 455, 605
HpaII CCGG 5 cut(s) 117, 124, 141, 158, 227
HphI GGTGA 2 cut(s) 113, 200
Hpy166II GTNNAC 2 cut(s) 440, 665
Hpy188I TCNGA 7 cut(s) 393, 430, 454, 478, 594, 604, 640
Hpy188III TCNNGA 1 cut(s) 623
Hpy8I GTNNAC 2 cut(s) 440, 665
Hpy99I CGWCG 1 cut(s) 693
HpyAV CCTTC 1 cut(s) 558
HpyCH4III ACNGT 4 cut(s) 80, 351, 662, 679
HpyCH4IV ACGT 2 cut(s) 627, 688
HpyCH4V TGCA 4 cut(s) 282, 332, 504, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 18, 577
HpyF3I CTNAG 3 cut(s) 423, 467, 477
HpySE526I ACGT 2 cut(s) 627, 688
Hsp92I GRCGYC 1 cut(s) 290
Hsp92II CATG 1 cut(s) 409
HspAI GCGC 1 cut(s) 290
KasI GGCGCC 1 cut(s) 289
KpnI GGTACC 1 cut(s) 548
Kzo9I GATC 4 cut(s) 388, 416, 470, 522
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 2 cut(s) 226, 344
MaeII ACGT 2 cut(s) 627, 688
MaeIII GTNAC 1 cut(s) 119
MalI GATC 4 cut(s) 390, 418, 472, 524
MboI GATC 4 cut(s) 388, 416, 470, 522
MboII GAAGA 4 cut(s) 204, 207, 256, 647
MfeI CAATTG 1 cut(s) 266
MflI RGATCY 1 cut(s) 522
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 4 cut(s) 144, 266, 462, 698
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 1 cut(s) 170
MseI TTAA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 5 cut(s) 117, 124, 141, 158, 227
MspR9I CCNGG 3 cut(s) 23, 174, 234
MunI CAATTG 1 cut(s) 266
Mva1269I GAATGC 1 cut(s) 284
MvaI CCWGG 3 cut(s) 23, 174, 234
MwoI GCNNNNNNNGC 2 cut(s) 18, 577
NarI GGCGCC 1 cut(s) 290
NdeII GATC 4 cut(s) 388, 416, 470, 522
NlaIII CATG 1 cut(s) 409
NlaIV GGNNCC 5 cut(s) 231, 291, 516, 537, 546
NmuCI GTSAC 1 cut(s) 119
PasI CCCWGGG 1 cut(s) 233
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 3 cut(s) 375, 455, 605
PkrI GCNGC 2 cut(s) 241, 334
PleI GAGTC 1 cut(s) 169
PluTI GGCGCC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 64
Psp6I CCWGG 3 cut(s) 21, 172, 232
PspEI GGTNACC 1 cut(s) 119
PspFI CCCAGC 3 cut(s) 203, 305, 335
PspGI CCWGG 3 cut(s) 21, 172, 232
PspN4I GGNNCC 5 cut(s) 231, 291, 516, 537, 546
PspPI GGNCC 2 cut(s) 155, 229
PstI CTGCAG 2 cut(s) 334, 613
PsuI RGATCY 1 cut(s) 522
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 4 cut(s) 93, 404, 546, 676
RsaNI GTAC 4 cut(s) 92, 403, 545, 675
SacI GAGCTC 1 cut(s) 64
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 2 cut(s) 240, 333
Sau3AI GATC 4 cut(s) 388, 416, 470, 522
Sau96I GGNCC 2 cut(s) 155, 229
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 3 cut(s) 23, 174, 234
SduI GDGCHC 1 cut(s) 64
SfcI CTRYAG 3 cut(s) 330, 357, 609
SfoI GGCGCC 1 cut(s) 291
SinI GGWCC 1 cut(s) 229
Sse9I AATT 4 cut(s) 144, 266, 462, 698
SsiI CCGC 4 cut(s) 186, 272, 435, 554
SspDI GGCGCC 1 cut(s) 289
SstI GAGCTC 1 cut(s) 64
StyD4I CCNGG 3 cut(s) 21, 172, 232
TaaI ACNGT 4 cut(s) 80, 351, 662, 679
TaiI ACGT 2 cut(s) 630, 691
TaqI TCGA 1 cut(s) 387
TasI AATT 4 cut(s) 144, 266, 462, 698
TatI WGTACW 1 cut(s) 91
TfiI GAWTC 3 cut(s) 375, 455, 605
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 4 cut(s) 356, 385, 618, 665
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 239, 332
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 447
TspRI CASTG 4 cut(s) 356, 385, 618, 665
VpaK11BI GGWCC 1 cut(s) 229
XcmI CCANNNNNNNNNTGG 2 cut(s) 29, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.